BAS LC-22C User manual

LC-22C
March, 2000
MF-9010 INSTRUCTION MANUAL
Temperature Controllers
for Liquid Chromatography
Bioanalytical
Systems, Inc
2701 Kent Avenue
West Lafayette
Indiana 47906

MANUFACTURER’S NOTE
The BAS logo is a registered trademark of Bioanalytical Systems, Inc.
Copyright March 2000
No portion of this manual may be reproduced without express, written permission of Bioanalytical Systems, Inc.
ALL RIGHTS RESERVED
Bioanalytical Systems, Inc.
2701 Kent Avenue
West Lafayette, IN USA 47906-1382
Phone: [phone]
Fax: [phone]

Section 1. PREFACE
The Bioanalytical Systems LC-22C Temperature Controller and LC-23A and LC-23C
Column Heating Compartments are versatile tools for the experimenter who desires precise,
reproducible temperature control for liquid chromatography.
The LC-22C Dual Channel Temperature Controller is a general purpose instrument capable
of regulating two independent devices, such as heating mantles, heat tapes, electrochemical
cell preheaters, and column heating blocks. The temperature control point is set with a
multi-turn front-panel potentiometer and the power applied to the heating unit is regulated
by a temperature sensor located in the device being controlled. In heating devices of BAS
manufacture, the temperature sensor is built into the instrument. To control other devices, a
universal probe (part no. EW-8116) is available for separate purchase.
The LC-23A Column Heating Compartment is used in conjunction with the LC-22C. This
instrument is simple in design and extremely durable. It accepts most commercially
available 15, 25 or 30 cm analytical columns. A solid aluminum inner compartment,
thermally isolated from the outer cover, encloses the entire column. A preheater coil is
included with each unit. The LC-23A can, in some instances, be altered to accommodate
wider diameter columns. This alteration would involve an additional charge for
customization. If you require modifications, please contact BAS customer service for a
quotation.
The LC-23C Cartridge Column Heating Compartment accommodates two cartridge format
columns up to 15cm in length. The LC-23C can be mounted within the BAS CC-5
Chromatography Compartment, which is part of the BAS 480 Liquid Chromatographs and
LC-44 Electrochemical Detectors. The LC-23C is also sold with or without the CC-5, the
latter as an upgrade to existing BAS detectors or BAS 480 systems.
The Cell Preheater option for BAS electrochemical detectors is also controlled by an
LC-22C. The preheater coil can accommodate the heating of stainless steel tubing between
the column and cell inlet.
Figure 1. LC-22C Front Panel.
SETPOINT
HEATER 1 SETPOINT HEATER 2
DISPLAY
SELECT
Temperature Controller
LC-22C
Section 1. PREFACE
1

Section 2. POWER REQUIREMENTS
Figure 2. LC-22C Back Panel.
LC-22C CONTROLLER This instrument can be operated from either 115V/50-60Hz or 230V/50-60Hz. The line
voltage selector for 115V or 230V operation is on the back chassis panel. The line voltage
must be selected before applying power to the controller.
A 3-prong power cable on the LC-22C must be connected to a standard, grounded power
outlet for safety. If the instrument is operated from a power outlet without a ground
connection, an appropriate adapter should be used and the ground connection of this
adapter should be securely fastened to an earth ground.
LC-23A and LC-23C HEAT-
ING COMPARTMENTS The LC-23A and LC-23C compartments are designed specifically for use with the LC-22C
Controller. Two cables (one for heater power and the other for the temperature sensor)
connect to the LC-22C to each heating device. If you have purchased a heating
compartment for use with an older model LC-22A, you may be unable to make the
connection directly to the controller without an additional adapter. A change in design
converted this from a 3-prong male plug to the current female plug. The adapter (EF-1575)
is available from BAS at an additional charge.
GND
LC-22C
Made in USA
GND
REMOTE
ADDRESS OUTPUT
PROBE 1 PROBE 2
HTR 2HTR 1 F1
F2
1234 21INH
Section 2. POWER REQUIREMENTS LC-22C CONTROLLER
2

Section 3. INSTALLATION
LC-22C CONTROLLER This section concerns installation for stand-alone operation of the LC-22C with the optional
universal probe. For operation of the other accessories, see subsequent sections.
1. Use the cable adapter (EF-1575) to convert the special safety receptacle on the back panel
of the LC-22C to a standard 3-prong socket. Plug the device to be controlled (e.g.,
heating mantle, tape, etc.) into this socket. If you only wish to measure temperature
and do not need to control it, you can skip this step.
2. Plug the cable leads from the stainless steel UNIVERSAL PROBE (EW-8116) into the
red, yellow, and black jacks on the back panel of the LC-22C labeled “PROBE 1" or
”PROBE 2". The black jack provides grounding for the instrument and must be
connected for safety purposes.
3. The liquid probe is usually accurate to within 1°C of the actual set-point when shipped.
The reproducibility or precision of the temperature set-point is ±0.1°C regardless of
the actual temperature. If greater accuracy is desired, follow the calibration procedure.
To perform the calibration, first remove the top cover of the LC-22C by removing the
four screws located on the sides of the top cover. Since both channel 1 and channel 2
are identical, calibrating instructions will be given for channel 1 adjustments, while
channel 2 adjustments will appear in parentheses ().
To calibrate the probe, place it in an ice bath and connect the probe leads to the
“PROBE 1" (”PROBE 2") jacks on the back panel. Turn the main power on and rotate
the display selector knob to “SENS 1" (”SENS 2"). Using a small screwdriver, rotate
the adjusting screw on the top of the potentiometer R45 (R56) for a reading of 00.0°
on the front-panel digital readout. Remove the probe from the ice bath. If a two-point
calibration is desired, go to step 5.
4. This completes the single-point calibration of the liquid probe. The top cover should be
reinstalled using the four retaining screws on the sides of the cover.
5. A two-point calibration can be made by placing the probe in boiling water and adjusting
R46 (R55) first to a display reading of 99.9, then to 00.0 (indicating 100.0°C) with the
center decimal point on. There could be a very slight interaction between the freezing
and boiling point adjustments, so additional adjustments to R45 and R46 (R56 and
R55) could be needed to obtain the highest degree of accuracy. Remove the probe
from the high temperature bath.
6. This completes the two-point calibration of the liquid probe. The top cover should be
reinstalled using the four retaining screws on the sides of the cover.
LC-22C CONTROLLER Section 3. INSTALLATION
3

LC-23A COLUMN HEATING
COMPARTMENT 1. Place the LC-23A heating Compartment in the desired location. Six holes with captive
6-32 threads are provided for secure mounting on your liquid chromatograph, if
desired. The holes are positioned to fit early model (pre 1986) BAS liquid
chromatography support stands.
2. Connect the LC-23A heater power cable to the power socket on the back panel of the
LC-22C Controller.
3. Connect the red, black and yellow plugs on the probe cable to the jacks marked “PROBE
1" or ”PROBE 2" on the LC-22C back panel.
4. Remove the cover on the LC-23A Column Heater by unscrewing the two cap screws.
Place the column in the recessed cavity and route the inlet and outlet tubing out of the
top and bottom slots. A special preheater block (EW-8146) is also supplied. Under
certain circumstances, dramatic band broadening may result if the preheater is not
utilized. Typically this becomes obvious when the temperature differential between
ambient and the desired set point exceeds 20-25°C.
If you plan to use several different styles of column endfittings, it is best to use
removable plastic fittings. BAS recommends a 2-piece PEEK finger-tight seal as
provided in BAS part no. MR-4409 (this includes a nut and ferrule). One piece plastic
fittings tend to loosen at higher temperatures.
Figure 3. Position of Mounting Points on LC-23A Heater.
Figure 4. LC-23A Column Heater with LC-23PH Preheater Installation.
2.01 5.00 6.00 3.37
0.50
0.75
0.50
8.89cm
4.92cm
4.12cm
10.79cm
9.36cm
39.37cm
2.03cm 2.54cm
1.93cm
PREHEATER
1.91 x 2.54cm
INLET DEPTH
1.89cm
CHANNEL WIDTH
0.685cm COLUMN
HEATER
BLOCK
SENSOR
LOCATION
ADAPTER FOR
25-30cm COLUMNS
OUTLET DEPTH
1.85cm
Section 3. INSTALLATION LC-23A COLUMN HEATING COMPARTMENT
4

LC-23C COLUMN HEATING
COMPARTMENT The LC-23C is specifically designed to fit within the BAS CC-5 Column Compartment. The
CC-5 is pre-drilled to accept a Rheodyne injection valve on the front panel. It has several
grooves on both sides for convenient access to external detectors.
If you are installing the LC-23C as an upgrade to a BAS electrochemical detector or BAS
480 Liquid Chromatograph, and already have a cell preheater installed in the CC-5, you
may wish to remove it. Routing the tubing from the LC-23C to the cell preheater and then
to the detector is difficult due to space limitations. The LC-23C alone will provide
satisfactory temperature control for the electrochemical cell if you keep the cover closed
during operation.
Before installing the LC-23C, give some thought to the configurations of the column(s) you
intend to use. Some configuration using compact BAS cartridge columns are diagrammed
in Figure 5. You can also leave the analytical column outside the LC-23C (at room
temperature) and heat a post-column reactor (tubing coil, packed bed) within the LC-23C.
Figure 5. Combinations of Column Formats Within the LC-23C.
POST-COLUMN
REACTOR
MIXING TEE
AND REAGENTS
ANALYTICAL
COLUMN
TO UV
DETECTOR
10cm
ANALYTICAL
COLUMN
15 OR 10cm
ANALYTICAL
COLUMN
3cm
POST-COLUMN
POST-COLUMN
REACTOR
ANALYTICAL
COLUMN AT
AMBIENT
15cm
ANALYTICAL
COLUMN
10cm
ANALYTICAL
COLUMN
LC-23C COLUMN HEATING COMPARTMENT Section 3. INSTALLATION
5

Installing the LC-23C in a CC-5 Compartment
1. Lower the CC-5 lid and remove the utility cover from the back panel of the CC-5 cabinet
(four screws).
2. On some models, pressed-in, threaded nuts will be located on the outside of the back
panel (Figure 6). These must be removed. DO NOT REMOVE IF THEY ARE ON THE
INSIDE OF THE CABINET. To remove external nuts, screw a long machine screw (6-32
thread) into the nut from the rear until it protrudes through the nut. Use pliers for
leverage and gently work the screw from side to side until the nut comes loose.
3. Remove the LC-23C cover and set aside. Push the heater block through the now open
utility access in the CC-5 back panel. On some models, the two registration posts on
the CC-5 inner panel will engage the LC-23C block for support.
4. Secure the LC-23C to the cabinet using four 6-32 x 1/2" machine screws and four 6-32
lock nuts. The lock nuts are not necessary if pressed-in nuts are available on the inside of
the CC-5 cabinet. The mounting plate should now be flush with the outside of the back
panel on the CC-5 cabinet.
Figure 6. Installation of the LC-23C in a BAS 480 or CC-5 Electrochemical Detector. (A) Location of four screws (B) Breaking off
the pressed-in nuts (C) Left Side View of Attachment Hardware.
6-32 LOCKNUT
(4 PLACES)
6-32 x 1/2" SCREW
(4 PLACES)
SCREW
LOCATIONS
A
B.
C.
CC-5
REAR CC-5 FRONT
Section 3. INSTALLATION LC-23C COLUMN HEATING COMPARTMENT
6

Making Plumbing Connections
For simple arrangements, use the innermost trough of the LC-23C. This location minimizes
detector and valve hookup tubing. If you are coupling columns together, the longer trough
will be more useful.
A special coil of tubing is provided with each LC-23C (see Figure 7). At your option, it may
be installed between the injection valve and column. The coil functions as a mobile phase
preheater, so that large temperature gradients at the head of the column are minimized. The
coil may be removed by unscrewing the central cap screw (5/32" hex).
It is advisable not to use a permanent ferrule on the outlet end of the preheater coil. A
removable high-pressure fitting provides an equivalent seal without irreversible attachment.
Use BAS part no. MR-4409.
Figure 7. Column Preheater Coil Installed in LC-23C. This Unit is Part of a BAS 480 Liquid Chromatography System with the
Electrochemical Cell and Cell Preheater Options Installed.
Leaks
Make sure that all fittings are leak-tight at the usual operating pressure before mounting the
insulating top cover.
Insulating Top Cover
The U-shaped top cover is insulated on three sides with a foam polymer. The cover is
asymmetric; the notch in the cover goes along the right side. Mount the cover at the rear
first and then drop the front in place.
HIGHLOW
PREHEATER POWER
LC-23C COLUMN HEATING COMPARTMENT Section 3. INSTALLATION
7

Section 4. OPERATION
For purposes of instruction, we will reference channel 1, although channel 2 is identical.
1. With the heater and probe cables disconnected, turn on the main power to the LC-22C.
2. Rotate the display selector knob to illuminate “SET 1" in the red display window.
3. Using the “SETPOINT HEATER 1" knob, select the desired temperature, which is between
ambient and 99.9°C.
4. Turn off the main power to the LC-22C and connect the heater to “HTR-1" and the red,
yellow and black probe plugs to the same color jacks of ”PROBE-1" on the back panel.
5. Turn on the main power to the LC-22C. The heater indicator LED will remain constantly
illuminated until the set point is reached, then it will dim or blink intermittently to
indicate maintenance at the desired temperature. You can also observe the measured
probe temperature by changing the display select switch to “SENS 1".
TEMPERATURE MEASURE-
MENT/CONTROL MODE
LC-23A or LC-23C Column Heaters
and Electrochemical Cell Preheater
1. Ensure that the three (3) leads from the column heaters or cell preheater are plugged into
the jacks marked “PROBE 1" or ”PROBE 2"
2. Turn on the LC-22C. Set the desired temperature (at least 5°C above ambient).
3. The measured temperature can be read on the front panel digital display of the LC-22C by
using the display selector knob. The temperature is also available as an analog voltage
at the screw terminals marked “OUTPUT + 1 -” or “OUTPUT + 2 -”, depending upon
the channel used. Each 100 mV (0.1 volt) is equivalent to 1°C. For example:
+8.92 volts = +89.2°C
-0.36 volts = - 3.6°C
This voltage may be recorded using a strip chart recorder. Set the full scale input of
the recorder to 10 volts and observe the marked polarity on the back panel.
4. The LED light will stay on continuously until the heater achieves the setpoint and then will
flash intermittently while maintaining this temperature. The actual temperature (within
the absolute accuracy of the probe) at any point in time will be displayed on the front
panel digital display of the LC-22C or the recording device.
Section 4. OPERATION TEMPERATURE MEASUREMENT/CONTROL MODE
8

Universal Liquid Probe
1. Ensure that the stainless steel probe is plugged into the jacks marked “PROBE 1" or
”PROBE 2" and observe the color code used on the jacks.
2. Turn the main power on to the LC-22C. Rotate the display selector knob to “SENS 1"
or ”SENS 2", depending upon which channel is being used for measurement. The
digital display will read the probe temperature in degrees C.
3. The measured temperature is available as an analog voltage at the “OUTPUT” screw
terminals on the back panel. This six position terminal strip is detachable: pull straight
back for release. Probe 1 outputs are the labeled “+ 1 -”, probe 2 outputs are labeled
“+ 2 -” on the back panel.
4. An analog voltage of 100 mV (0.1 volt) is equivalent to 1°C. For example:
+8.92 volts = +89.2°C
-0.36 volts = - 3.6°C
This voltage may be recorded using a strip chart recorder. Set the full scale input of the
recorder to 10 volts. Observe the marked polarity.
5. To control the temperature while measuring it, first turn off the main power to the LC-22C.
Connect the power cable of the heating device (75 watts maximum) to the appropriate
heater jack, i.e., “HTR 1" if ”PROBE 1" is the corresponding sensor channel.
Turn the main power on and select the corresponding channel setpoint LED. Rotate the
setpoint heater knob to obtain the desired control temperature. The actual probe
temperature can be observed by changing the display selector knob to the controlled
channel’s sensor LED, marked “SENS 1" or ”SENS 2".
TEMPERATURE MEASUREMENT/CONTROL MODE Section 4. OPERATION
9

Section 5. SAFETY AND MAINTENANCE CHECKS
The LC-22C, LC-23A, LC-23C and cell preheater are all essentially maintenance free
instruments. BAS does advise that you attempt to keep these pieces in good condition by
following these suggestions:
1. Avoid corrosive laboratory environments.
2. Don’t stack heavy items on signal cables or allow them to become crimped during use
or storage.
3. Clean up spills inside column heaters immediately.
4. Avoid contact of organic solvents with the insulating material inside the LC-23C cover.
The Universal Probe is based on a reliable solid state device capable of generating a
predictable current related to the temperature of its environment. To check the integrity of
this probe, apply the following test:
1. Expose probe to ambient temperature and disconnect any heating devices from the
LC-22C.
2. Turn on the LC-22C power.
3. Set the temperature setpoint to a value greater than ambient (allow at least 1°C difference).
The “HTR” LED indicator on the LC-22C should be on continuously.
4. An open circuit anywhere in the probe, cable or across its input jacks will automatically
shut down the heater circuit. This feature was incorporated to protect against thermal
“runaway” and should be tested periodically. Perform the following check:
A. Plug in the probe, expose to ambient air temperature and turn on the
power to the LC-22C.
B. Create an open circuit in the probe by unpluging the red or yellow lead
from the chassis jacks on the LC-22C. Using the digital display
selector knob, illuminate the tested channel “SENS 1" or ”SENS 2"
LED. A digital reading of approximately .3.9.8 degrees (-13.98 volts at
the OUTPUT screw terminals) will be shown. Note that the left, center
and right decimal points are illuminated indicating an open circuit.
C. Remove the probe entirely and place a jumper cable across the red and
yellow PROBE INPUT jacks to create a closed circuit. A digital reading
of approximately 2.7.9 degrees (+12.79 volts at the OUTPUT screw
Section 5. SAFETY AND MAINTENANCE CHECKS
10

terminals) will be shown. Note that the center and right decimal points
are illuminated indicating a closed circuit.
If your unit fails either step 3 or 4, please contact the BAS Service Coordinator to return
both the LC-22C and the probe used to make these tests for service. Do not return any
equipment without first obtaining a Return Authorization number from BAS.
Section 5. SAFETY AND MAINTENANCE CHECKS
11

Section 6. SPECIFICATIONS
Temperature Setpoint
0.00°C to 99.9°C in 0.1°C increments
Temperature Measurement
-50°C to 115°C
Readout
Front panel LED
Max. Load
75 watts, each channel
Repeatability
0.1°C with the same temperature probe.
Output Temperature Calibration
Output is calibrated to 100 mV per °C.
Rear Panel
Probe input jacks, output temperature jack,
output power socket heaters, line voltage selector.
Heater Power
Heater power is controlled by zero crossing,
solid state relays for noise-free operation.
Fuses
For 115V, 2A Slo Blow,
For 230V, 1A Slo Blow
Section 6. SPECIFICATIONS
12