BestScope BS-2025M User manual

To ensure the safety and obtain satisfactory performance, please study this operation
instruction thoroughly before your operation.
Biological Microscope
Model: BS-2025M/B/T
Instruction Manual

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Contents
1. Purpose .....................................................................................................2
2. Name of Components...............................................................................2
3. Specifications ............................................................................................3
4. Installation ................................................................................................5
5. Operation ..................................................................................................6
6. Maintenance ...........................................................................................11
7. Troubleshooting......................................................................................14
8. Outfits .....................................................................................................16

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1. Purpose
BS-2025 series biological microscopes are designed for teaching, clinical verify.
This series microscopes with original style, steady structure, convenient
operation and clear image is suitable for observing various biological specimens,
and mostly applied in school, college, hospital and family.
2. Name of Components
Fig. 1 BS-2025M Monocular Microscope

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Fig. 2 BS-2025B Binocular Microscope
3. Specifications
3.1 Total magnifications
Objective
Total Magnifications
Eyepiece
4X
10X
40X
100X
10X/18mm
40X
100X
400X
1000X
16X/11mm
64X
160X
640X
1600X
3.2 Objectives
Working Distance
Objective
Objective
Numerical
Aperture
(N.A.)
Objective Field
(Field Number
ф18)
Resolving
Power
Achromatic
Plan
4X
0.10
4.5mm
2.8µm
13mm
16mm
10X
0.25
1.8mm
1.1µm
6.3mm
2mm
40X (S)
0.65
0.45mm
0.42µm
0.44mm
0.66mm
100X
(Oil) (S)
1.25
0.18mm
0.22µm
0.3mm
0.42mm
3.3 The other specification
3.3.1 Mechanical tube length: 160mm
3.3.2 Conjugate distance: 195mm

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3.3.3 Head: Monocular, Inclined 30°, Rotable 360°, Eyepiece can be fixed,
Anti-fungal systems.
Seidentopf binocular head 30°, Rotable 360°,
Interpupillary adjustable distance is 55-75mm,
Diopter adjustable range±5, Anti-fungal systems.
3.3.4 Nosepiece: Quadplex nosepiece
3.3.5 Mechanical stage: Size 142mm×134mm
X-Y travel 50mm×70mm
3.3.6 Focusing systems: Coaxial coarse and fine focusing knobs, Coarse stroke
22mm, Fine division 2µm, Condenser up-down range
25mm
3.3.7 Condenser: Abbe condenser, N.A. 1.25, Adjustable aperture,
Aperture center is adjustable.
3.3.8 Mirror: Mirror diameterф52mm (optional)
3.3.9 Filter: Built in blue filer, ф38.5mm
3.3.10 Anti-fungal systems: Eyepiece and objective is anti-fungal, anti-fungal
system built in head.
3.3.11 Electric components: Input voltage AC85-265V, 50/60Hz
Output voltage DC1.2-6V
6V/20W halogen lamp
Rotation potentiometer with power switch
Fuse 2A ф5×20

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4. Installation
BS-2025 series microscopes are packed as integrate, and it is convenient for
installation.
Open the package carton, and insert eyepiece into the eyepiece tube of the
head (eyepiece for monocular microscope has been inserted before packing). If
objectives don’t be fixed, please take objectives out from objectives packing
box, and drive them into the holes of nosepiece orderly and tightly according to
the magnification.
Take out the oil bottle and dust cover, and place it properly for convenient use.

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5. Operation
5.1 Electric components
Make sure the supply voltage meets the instrument’s requirement, then plug in
it. Rotation potentiometer with power switch used by the instrument is for
protecting lamp.
5.2 Placing specimen (Shown in Fig.3)
Please place specimen on the stage, and the face with cover glass should be up,
then clip it with clamp. Clipping specimen should be careful in order to avoid
spoiling glass. Place specimen on the stage levelly, and make it into optical path
by adjusting vertical moving knob and horizontal moving knob.
5.3 Condenser (Shown in Fig.3)
5.3.1 Condenser up-down
Turn the condenser up-down knob to adjust
the distance between condenser front and
the specimen, and change the equal
Mirror
Bracket
Mirror Base
Fig.4 Mirror
ClampHandleSpecimen Clamp Condenser
Vertical
MovingKnob
Horizontal
MovingKnob
Handleof
IrisAperture
Condenser
Up- downKnob
Fig.3 StageAndCondenser
Diopter Ring
Eyepiece
Interpupillary
DistanceScale
Fig.5 Interpupillary Distance
Adjustment And Diopter Ring
EyepieceTube

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illumination to obtain the best brightness.
5.3.2 Iris aperture adjustment
Stir the handle of iris aperture of condenser to adjust size of iris aperture for
adjusting the contrast of the specimen.
5.4 Interpupillary distance and diopter adjustment (Shown in Fig.5)
5.4.1 Interpupillar distance is different for everyone, so interpupillary distance
should be adjusted before using binocular microscope. Please take the two
eyepiece tubes to turn until the bright rings observed by two eyes are in
superposition entirely.
▲BS-2025M monocular microscope don’t need to be adjusted.
5.4.2 Diopter adjustment
As focusing for binocular, user should observe right eyepiece with right eye,
and make the right eyepiece clear by focusing adjustment, then observe the
left eyepiece, at the same time, adjust the diopter ring of the left eyepiece tube
to make the image of left eyepiece clear as same as the right eyepiece.
5.5 Focusing adjustment
Usually 10X objective should be used for focusing adjustment first. Take 10X
objective into bright path, then observe the specimen with right eyepiece by
right eye and turn coarse focusing knob to find the image, then turn slowly fine
focusing knob to make the image clear.
All objectives of our microscope are precisely adjusted, and approximate clear
image should be found with the other objectives after getting the clear image
with 10X objective. If the image isn’t enough clear with the other objectives,

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please turn fine focusing knob slightly to find the satisfactory image.
▲Don’t turn left and right coarse and fine focusing knobs with different
direction with power at the same time, if so, the focusing system will be
damaged.
▲Don’t directly pull objective to turn nosepiece when user changes the
different objectives, if so, optical
quality of microscope possibly be
affected. The right way is to take the
tooth-like part of the nosepiece to
turn it, and make the objective into
correct position and into the bright
path.
5.6 Usage of the tension adjustment ring (Shown in Fig.6)
The tension adjustment ring can adjust the tension of the coarse and fine
focusing unit to prevent the stage from sliding down automatically and to
improve the comfort of operation. Rotation clockwise makes tension decrease,
and by contraries, rotation counterclockwise makes tension increase.
5.7 Usage of immersion oil objective
The 100X objective of BS-2025 series microscopes can be used for observation
even if without immersion oil. However, adding moderate immersion oil
between the front lens of 100X objective and the cover glass of specimen can
make the image clearer. Please pay attention that air bubble and impurity can’t
be in the immersion oil, otherwise, the image would be affected.
Fig.6 Coarse A F Fnd ine ocusing
Left Coarse
Left Fine
Right Fine
Right Coarse
Tension
FocusingKnob
FocusingKnob
FocusingKnob
Adjustment Ring
FocusingKnob
KnobsAnd Tension Adjustment Ring

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First of all, take 40X objective which has been focused well out from bright path,
then take 100X objective into bright path. At this time, nosepiece or stage
moving knob should be turned slightly, and also slightly turn fine focusing knob
to clear away the air bubble of immersion oil, otherwise, the air bubble would
affect the image badly.
▲After immersion oil used, the oil of specimen and the microscope surface
should be soon cleaned by absorbent cotton, lens paper, gauze or soft cotton
cloth with moderate mixture of pure industrial alcohol and ether (proportion
1:4)
▲Standard thickness 0.17mm cover glass should be chosen when high times
objective used, and thickness error should be within 0.01mm, otherwise, image
definition would be affected.
5.8 Mirror (Shown in Fig.4)
Mirror of BS-2025 series microscopes is optional, and it is used when electrical
power isn’t supplied.
Mirror doesn’t be suggested as illumination source when 100X objective used.
Take light collector down from base, then take mirror on base. Turn mirror to
make reflecting face to the light source, and make reflecting face and horizontal
face as 45°approximately to make the light to specimen as possible. The mirror has
two sides, one is a plane mirror, and the other one is a concave one. Concave
mirror is suitable for large-area outside light source, such as bright sky.
5.9 Points of attention after using
5.9.1 Power of microscope should be turned off after using, and the plug

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should be pulled down. If immersion oil used, please clean objective and
specimen soon. Finally, cover microscope with dust cover.
5.9.2 Please take eyepiece and objectives out from microscope if user will stop
using it for a long time, and place eyepiece and objectives into drier with drying
agent. Cover microscope with dust cover.

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6. Maintenance
6.1 Clean microscope
6.1.1 Don’t touch the lens with hand, Dust on lens should be cleaned by soft
brush or absorbent cotton or cleaned by absorbent cotton, lens paper with the
mixture of alcohol and ether (proportion 1:4).
6.1.2 Alcohol and ether all are burnt easily, please take them away from fire. Be
careful for turn on and off power.
6.1.3 Don’t clean painted metal and galvanizing metal with organic solvent such
as alcohol, ether or the mixture of the both. Silicon cloth or soft cleaning
preparation is suggested to clean it.
6.1.4 Plastic should be cleaned by soft cloth with clear water.
6.2 Environment of using and placing
6.2.1 Microscope should be used and placed in a cool, dry, non-dust, non-shake
and non-corrosive gases environment.
6.2.2 Microscope should be used in environment of indoor temperature
0°-40°C and maximum relative humidity 85%.
6.2.3 Removing humidity equipment is suggested to be installed when
microscope used in heavy humidity area to avoid fungus and mist damage
instrument.
6.2.4 Please pay attention to prevent microscope from violent shake and
vibration in application and in carrying. Don’t drag it on the surface of
worktable to avoid damage microscope and worktable.

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6.3 Replacement of bulb (Shown in Fig.7)
6.3.1 Turn off power, and pull out plug.
6.3.2 Wait the bulb become cool.
▲Please be sure that the bulb is cool, then follow by the operations.
6.3.3 Lay aside the microscope reliably, unscrew the knurled thumb screw of
the lamp housing cover on the underside of base.
6.3.4 Pull over the lamp housing cover.
6.3.5 Pull out the bulb should be replaced, hold a new bulb with silk cloth to
avoid fingerprint and dust affect bulb brightness and service life, and insert
fully the contact pins into the
bulb socket.
6.3.6 Close the lamp housing
cover, and screw the knurled
thumb screw.
▲After working for above 10
U screwKnurledn
LampAdjustment Knurled
Pull Over
Bulb
HousingCover,
ReplaceBulb,
CloseCover
TheLamp
Cover
ThumbScrew
ThumbScrew
Fig.7 Replacement of Bulb
Power Wire FuseCap
Fig.8 Replacement of Fuse

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hours continuously, better cut off the microscope about 30 minutes.
6.4 Replacement of fuse (Shown in Fig.8)
6.4.1 Cut off power of microscope, and pull out the plug.
6.4.2 Unscrew fuse cap in the back of base, take out old fuse.
6.4.3 Replace a new fuse, then screw the fuse cap.
6.5 Stop to use microscope, please cut off power, cover the dust cover, and
place it in a cool and dry environment.
6.6 B-LED biological microscope
6.6.1LED illumination specification:
Available LED using time: Above
10,000 hours
Working Voltage: 3.1V
Maximal working electrical current:
30mA
Maximal power dissipation: 120mW
Luminous intensity: Above 6cd
6.6.2LED bulb replacement:
Cut off power: Pull out the power plug off socket, and confirm power cut off
Put out bulb cover: Put out the bulb cover off the up-cover of the base LED
bulb replacement: Pull up the LED bulb with plug and out off the bulb socket,
then insert the replacing bulb with its plug into the bulb socket
Bulb Cover
LED bulb
Bulb plug
Bulb socket

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7. Troubleshooting
In the period of using BS-2025 series microscopes, if there is any trouble occurs,
please referring to the following sheet listing some common troubleshooting，
and resolve them.
Trouble
Causation
Remedy
Plug is unreliable
Plug in again
Bulb is broken
Change bulb
Switch on but bulb dark
Fuse is broken
Change fuse
Bulb is unstable
Insert it again
Bulb is flickering or
brightness is unsteady.
Bulb is broken
Replacing bulb
Bulb specification doesn’t
meet the requirement.
Replacing bulb
Brightness isn’t adjusted
correctly
Adjust rotation
potentiometer
Objective isn’t in correct
position
Make the objective in
correct position.
The size of iris aperture is too
small.
Adjust the size of iris
aperture.
Lens (objective, eyepiece,
condenser, light collector) has
dust.
Clean it
Brightness of view field
isn’t enough or is
uneven.
Position of condenser is too low
Higher condenser
Cover glass of specimen
doesn’t meet the requirement
Use required thickness
cover glass (0.17mm)
Cover glass of specimen isn’t in
up direction
Place specimen correctly
Surface of objective lens is
dirty (especially it is easy for the
front lens of 40X
objective to dip in immersion
oil)
Clean it
Immersion oil isn’t used for
100X objective (oil)
Use immersion oil
Immersion oil doesn’t meet
the requirement
Use immersion oil
supplied by us
There is bubble in immersion oil
Clear the bubble way
Size of iris aperture isn’t
proper
Adjust the size of iris
aperture
Image isn’t clear
(contrast or definition
isn’t enough).
Position of condenser is too low
Readjust the position of
condenser

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Objective isn’t in correct
position.
Make the objective in
correct position.
One side of image is dark
or image is moving as
focusing.
Specimen isn’t placed
correctly
Place specimen levelly on
stage and clip it with
clamp.
Cover glass of specimen isn’t
in up direction.
Place specimen correctly
Objective touches
specimen as changing
low times objective to
high times objective.
Cover glass doesn’t meet the
Requirement.
Use required thickness
cover glass (0.17mm)
Image observed by two
eyes aren’t in
superposition entirely.
Interpupillary distance isn’t
adjusted correctly.
Adjust interpupillary
distance according to two
eyes.
It is easy for eyes to be
tired during observing.
Diopter isn’t adjusted
correctly
Readjust diopter

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8. Outfits
Items
Specification
BS-2025M
BS-2025B
BS-2025T
WF 10X-18 mm
●
●●
●●
WF 10X-18 mm(With Pointer)
○
○
○
WF 16X-13 mm
○
○○
○○
Eyepiece
WF 10X-18 mm(Reticule 0.1 mm)
○
○
○
4X
●
●
●
10X
●
●
●
40X(S)
●
●
●
100X/1.25 (Oil) (S)
●
●
●
20X
○
○
○
Achromatic
Objective
60X (S)
○
○
○
4X
○
○
○
10X
○
○
○
40X/0.65 (S)
○
○
○
100X/1.25 (Oil) (S)
○
○
○
20X
○
○
○
Plan Objective
60X(S)
○
○
○
Monocular Head
Inclined 30°, Rotatable 360°
●
Seidentopf
Binocular Head
Inclined 30°,Rotatable 360°,
Interpupilary Distance 50-75mm
●
Seidentopf
Trinocular Head
Inclined 30°,Rotatable 360°,
Interpupilary Distance 50-75mm,
Light Distribution: 20:80
●
Nosepiece
Quadplex
●
●
●
Focusing Knobs
Coaxial Coarse and Fine Focusing Knobs
●
●
●
Mechanical Stage
Stage Size: 120mmX115mm,
Travel: 50mmX70mm
●
●
●
Condenser
Abbe N.A. 1.25(Iris Diaphragm)
●
●
●
Dark Field Ring
Available For 4X-40X Objective
○
○
○
Polarizing
Attachment
Analyzer /Polarizer
○
○
○
Mirror
Plano-concave mirror
○
○
○
C-mount
1X/ 0.5X （Focus Adjustable）
○
LED Illumination Systems (non-rechargeable)
●
●
●
LED Illumination Systems (rechargeable)
○
○
○
Base
6V 20W Halogen Lamp, Wide Voltage: 100V-240V
○
○
○
Note: "●"In Table Is Standard outfits, "○" Is Optional Accessories.
Packing Size: 337mm×242mm×417mm Gross Weight: 7 kgs Net Weight: 5.5 kgs