BestScope BS-2043 Series User manual

Version No.: V1.0
Biological Microscope
BS-2043 Series
Instruction Manual
BS-2043B BS-2043T
This instruction manual is for the operation guide, troubleshooting and
maintenance to the BS-2043 series biological microscope. Please study this manual
thoroughly before operating, and keep it with the instrument. The manufacturer
reserves the rights to the modifications by technology development. On the basis
of operation ensured, technical specifications may be subject to changes without
notice.

BestScope International Limited
2
Contents BS-2043 Series
Before Use
1.Components………………………………..…………..………………..………..………………………..1
2. Assembling………………..……………………..……………..………..…………………………………3
2-1Assembling Scheme…………………………..…..……..……………………….……………...3
2-2Assembling Steps ...……………………..……..………………..……………………………....4
3. Operations……………….…………………………..……….….………………..………………………….6
3-1Set Illumination………….………….…………..………..………………………………………..6
3-2 Place the Specimen Slide ..……….…………..………..……….…………………………....6
3-3 Adjust the Focus …………………………………………..………. ….……………………....6
3-4 Adjust the Focusing Tension …………………………..………..……………………..….7
3-5 Adjust the Interpupillary Distance…….………………..………..……………………….….7
3-6 Aperture Diaphragm……...…………………………………..………..……..…………………….7
3-7Use the Oil Objective (100X)….……………………………..………..………………………..….8
3-8 Use the Filter ………………………………………..………..………..…………………………..….8
3-9 Assembling and using of the TV Device..……………………..………..……………….....8
3-10 Assembling of the Photography Device……………………..………..……………….....9
3-11 Use the Storage Box ………………………………………..………..………..…………….....9
4. Troubleshooting….…………………………………..………..………..……………………………………10

BestScope International Limited
3
Before Use BS-2043 Series
1. Operation Notice
1. As the microscope is a high precision
instrument, always operate it with care, and avoid
physical vibrations.
2. Do not expose the microscope in the sun
directly, either not in the high temperature, damp,
dust or acute shake. Make sure the worktable is
flat and horizontal.
3. When moving the microscope, keep holding the
rear cover hand clasp①and the front end of
microscope body②with each hand. Handle with
care. (See Fig. 1)
★It will damage the microscope by holding the
stage, focusing knob or head when moving.
4. Connect the microscope to the ground to avoid
lightning strike.
5. For safety, make sure the power switch ③is at
“O” (off) and power it off before replacing the
bulb, and wait until the bulb and base cool down
completely (see Fig. 2).
★Bulb selected only: single 3W LED
6. Wide voltage range is supported as 100~240V.
Additional transformer is not necessary. Make
sure the power supply voltage is in this range.
7. Use the special power adapter supplied by our
company.
Fig.1
Fig.2

BestScope International Limited
4
BS-2043 Series
2. Maintenance
1. Wipe the lens gently with a soft tissue. Carefully wipe off the oil marks and fingerprints
on the lens surfaces with a tissue moistened with a small amount of 3:7 mixture of alcohol and
ether or dimethylbenzene.
★As the alcohol and ether is flammable, don’t place these chemicals near to fire or fire
source. For example, when turning on or turning off the electrical device, please use these
chemicals in a ventilated place.
2. Don’t use organic solution to wipe the surfaces of the other components. Please use the
neutral detergent if necessary.
3. If the microscope is damped by liquid when using, please power it off immediately and
wipe it dry.
4. Never disassemble the microscope, otherwise the performance will be affected or the
instrument will be damaged.
5. After using, cover the microscope with a dust cover.
3. Safety Sign
Sign
Signification
Study the instructions before use. Unsuitable operation would lead to
person hurt or instrument faulty.
|
Main switch ON
O
Main switch OFF

BestScope International Limited
5
1. Components BS-2043 Series
Eyepiece
Objective
Stage
Condenser
Fixed Koehler
Illuminator
Condenser
Up-down Knob
Fine
Focusing
Knob
Coarse
Focusing
Knob
Body Group
Binocular Head
FocusArm
Tension
Adjustment
Ring

BestScope International Limited
6
BS-2043 Series
Eyepiece
Trinocular Head
Fine
Focusing
Knob
Coarse
Focusing
Knob
FocusArm
Body Group
Lock Screw
Light
Adjustment
Knob
Power
Switch
Nosepiece
Vertical
Adjustment
Knob
Horizontal
Adjustment Knob
Condenser
Stage
Objective

BestScope International Limited
7
2. Assembling BS-2043 Series
2-1 Assembling Scheme
Following is the Assembling Scheme, and the numbers denote the assembling order.
★Before assembling, make sure there is no dust, dirt or other materials which will disturb it.
Assemble carefully and do not scrap any part or touch the glass surface.
Eyepiece
Objective
Condenser
Power Cord

BestScope International Limited
8
BS-2043 Series
2-2 Assembling Steps
2-2-1 Assemble the Condenser
1. Rotate the coarse focusing knob①according
to the direction shown in the figure, to raise the
stage to the highest position (see Fig.3).
2. Fully loosen the condenser lock-screw②.
3. Insert the condenser into the hole of base
according to the arrowhead pointed, until the
condenser is aligned with the base, and rotate the
condenser to make the handle face forward.
4. Tighten the condenser lock-screw②of
condenser, then raise the condenser with the
up-down knob to the highest position.
2-2-2 Assemble the Objective
1. Rotate the coarse focusing knob①to lower
the stage to a suitable position (see Fig. 4).
2. Then install the objectives into the
nosepiece②from the lowest magnification to
the highest in clockwisedirection.
★When operating, first use the low
magnification objective (4X or 10X) to search for
specimen and focus, and then replace with high
magnification objective to observe.
★When replacing the objective, rotate the
objective nosepiece until it sounds “ka-da”, to
make sure the objective wanted is in the center
of optical path.
2-2-3 Assemble the Eyepiece
1. Take down the cover of eyepiece tube①.
2. Insert the eyepiece② into the eyepiece tube,
until it touches the surface.
3. Tighten eyepiece with M2.5 inner hexagon
lock-screw③(see Fig.5).
Fig.5
Fig.4
Fig.3

BestScope International Limited
9
BS-2043 Series
2-2-4 Connect the Power Cord
★Don’t use strong force when the power cord is
bended or twisted, otherwise it will be damaged.
1. Make sure the power switch is at “O”(OFF)
before connecting the power cord.
2. Insert the connector①of power cord into the
power socket②, and make sure it connects well
(see Fig.6).
3. Insert the other connector into the socket of
power supply, and make sure it connects well.
★Use the special wire supplied by our company.
If it’s lost or damaged, choose one in the same
specifications.
★Wide voltage range is supported as 100~240V.
★Connect the power cord appropriately to
make sure the instrument is connected to
ground.
Fig.6

BestScope International Limited
10
3. Operations BS-2043 Series
3-1 Set Illumination
1. Put through the power and turn on the main
power switch to “—”(turn on) on the side.
2. Adjust the light adjustment knob②until the
illumination is comfortable for observation.
Rotate the light adjustment knob in clockwise to
raise the voltage and brightness. Rotate the light
adjustment knob in counterclockwise to lower the
voltage and brightness (see Fig.7).
3-2 Place the Specimen Slide
1. Push the wrench①of the specimen holder
backwards.
2. Place the cover glass of slide②faced upwards
into the clip, loosen the wrench①and clamp the
slide (see Fig. 8).
3. Rotate the X and Y-axis knob③of the stage,
and move the specimen to the center (aligned
with the center of objective).
3-3 Adjust the Focus
1.Rotate the condenser up-down knob①
according to the direction shown in the figure, to
raise it to the highest position.
2. Move the objective 4X into the optical path.
3. Observe the right eyepiece with right eye,
rotate the coarse focusing knob②until the
specimen outline appears in the view field (see
Fig. 9).
4. Rotate the fine focusing knob③ for clear
details.
★The position screw ④can avoid the
objective touching the clips.
Fig.8
Fig.7
Fig. 9

BestScope International Limited
11
BS-2043 Series
3-4 Adjust the Focusing Tension
If the handle is very heavy when focusing or
the specimen leaves the focus plane after
focusing or the stage declines itself, please adjust
the tension adjustment ring① (see Fig. 10).
To tighten the focusing arm, rotate the tension
adjustment ring① according to the arrowhead
pointed; loosen it in the reverse direction.
3-5 Adjust the Interpupillary Distance
When observe with two eyes, hold the base of
the prism and rotate them around the axis until
there is only one field of view.
“①” on the eyepiece base points to the scale
②of interpupillary indication, means the value
of interpupillary distance (see Fig. 11).
Adjustable range: 47-75mm.
★Remember your interpupillary distance for
further operation.
3-6 Aperture Diaphragm
1. The aperture diaphragm decides the numerical
aperture of the illumination system. Only when
the N.A. of illumination system is matching with
the N.A. of the objective, it can obtain better
resolution and contrast, and also increase the
depth of field.
2. As the contrast is usually low, rotate the
diaphragm adjust ring③to make the arrowhead
pointed to the related magnification position on
condenser base④, namely, to adjust the
condenser aperture diaphragm to be 70%-80% of
the N.A. of objective. The eyepiece can be taken
off when it’s necessary to observe from the tube.
Adjust the ring③to adjust the proportion (see
Fig. 12).
Fig.10
Fig.11
Fig.12

BestScope International Limited
12
BS-2043 Series
3-7 Use the Oil Objective (100X)
1. Use the 4X objective to focus the specimen.
2. Place a drop of oil①on the specimen
observed (see Fig. 15).
3. Rotate the nosepiece counterclockwise and
rotate the oil objective (100X) into the light path.
Then use the fine focusing knob to focus.
★Make sure there is no air bubble in the oil for
fear affect the image.
A. Move the eyepiece to examine the air bubble.
Open the aperture diaphragm and field
diaphragm fully and observe the edge of the
objective from the tube (It seems round and
bright).
B. Rotate nosepiece slightly and swing the oil
objective for some times to remove the air
bubble.
4. After using, wipe the front lens with a tissue
moistened with a small amount of 3:7 mixture of
alcohol and ether or with dimethylbenzene. Wipe
off the oil on the specimen.
★Don’t put another objective to the light path
before the oil is wiped to avoid wetting the dry
objective.
★Too much dimethylbenzene would dissolve
the lens’s stickiness.
3-8 Use the Filter
Filter can make the background be more
suitable and increase the contrast. (see Fig. 14).
Put the filter into the groove of condenser
★There are four colors of filter: blue, green,
yellow and white.
★Place the filter’s rough side downward.
3-9 Assembling and using of the TV Device
1. Take down the dust-cover②of the TV
adapter③. Install the CCD or camera into the
C-mount interface and tighten.
2. Loosen the lock screw① of trinocular head,
take out the dust-cover②. (See Fig. 17)
3. Insert the TV adapter③into the trinocular
head according to the figure and screw down the
lock screw①.
Fig.13
Fig.14
Fig.15

BestScope International Limited
13
BS-2043 Series
4. For binocular observation, after the image is
clear, observe the image of CCD. If the image is
unclear, rotate the TV adapter③for focusing
until it is clear.
3-10 Assembling of the Photography Device
1. Loosen the lock screw① of trinocular head,
take out the triplet dust-cover②.
2. Insert the photography device into the
trinocular head as shown in the figure, and screw
down the lock screw① (see Fig.16).
3. Loosen the lock-screw③ of the photography
adapter and take down the photography adapter
④ (See Fig. 17).
4. Insert the 3.2X photography eyepiece⑤ into
the eyepiece base⑥, then install the
photography adapter④and tighten the
lock-screw③.
5. After the image is clear through observation,
then operate the photography device according to
its instructions.
3-11 Use the Storage Box
When the microscope is not in use, the power
cord can be placed in the storage box. Press the
position①of the storage box cover with thumb.
When you hear a click, push up and put the
power cord and wrench into the storage box, and
then close the back cover. (See Fig.18, 19)
Fig.17
Fig.16
Fig.18
Fig.19

BestScope International Limited
14
4. Troubleshooting BS-2043 Series
As the performance of microscope can’t play fully due to unfamiliar operations, the table below
can provide some solutions.
Problem
Cause
Solution
1. Optical Part
(1) The LED light is bright, but
it’s dark in the field of view.
Field diaphragm is not large enough.
Enlarge the field diaphragm.
Condenser is too low.
Adjust the position of condenser.
(2) The edge of the field of view
is dark or not even.
The nosepiece is not in the right
position.
Turn the nosepiece into the right
position.
Stain or dust has accumulated on the
lens such as condenser, objective, or
eyepiece.
Clean the lens.
(3) Stain or dust is observed in
the field of view.
Stains have accumulated on the
specimen.
Clean the specimen.
Stains have accumulated on the lens.
Clean the lens.
(4) Unclear image
There’s no cover glass on the
specimen.
Add the cover glass.
The cover glass is not standard.
Use a standard cover glass with
thickness δ0.17mm.
The cover glass faces down.
Adjust it.
The immersion oil has accumulated
on the dry objective.
Clean it thoroughly.
The immersion oil is not used for oil
objective.
Use immersion oil.
Air bubble is in the immersion.
Get rid of the air bubble.
Use wrong immersion oil.
Use a correct one (cedar oil).
The aperture diaphragm is not
opened correctly.
Adjust the aperture diaphragm.
Stain or dust has accumulated on the
inlet lens of eyepiece.
Clean the lens.
The condenser is too low.
Adjust the condenser.
(5) One side of the field of view
is dark or the image moves
while focusing.
The specimen is not fixed.
Fix the specimen.
The nosepiece is not in the right
position.
Turn the nosepiece into the right
position.
Condenser centered incorrectly.
Center the condenser.

BestScope International Limited
15
BS-2043 Series
Problem
Cause
Solution
(6) The eyes feel tired easily.
The right field of view doesn’t
superpose with the left.
Interpupillary distance is wrong.
Adjust the interpupillary
distance.
Eyepieces for the left eye and right
eye are different.
Use the same eyepiece.
2. Mechanical Part
(1) Cannot get the objective
focused in high magnification.
The cover glass faces down.
Put the cover glass to face up.
The cover glass is too thick.
Use a standard cover glass with
thickness δ0.17mm.
(2) The objective touches the
cover glass while turning the
nosepiece from low to high
magnification.
The cover glass faces down.
Put the cover glass to face up.
The cover glass is not standard.
Use a standard cover glass with
thickness δ0.17mm.
(3) Coarse focusing knob is too
tight.
Tension knob is too tight.
Loosen it a little.
(4) Stage declines itself, cannot
stay on the focal plane when
observing.
Tension knob is too loose.
Tighten it a little.
(5) Coarse focusing knob can’t
rise.
The limit stop knob is locked.
Loosen the knob.
(6) Coarse focusing knob can’t
decline.
The base of the condenser is too low.
Raise the base.
(7) Cannot move the slide
smoothly.
The slide is not fixed correctly.
Adjust it correctly.
The movable specimen holder is not
fixed properly.
Adjust it correctly.
(8) The image moves obviously
when touching the stage.
The stage is fastened incorrectly.
Fasten the stage correctly.
3. Electrical Part
(1) The LED light does not work.
No power supply.
Check the connection of the
power cable.
The LED bulb is not installed
correctly.
Install it correctly.
The LED bulb is burnt out.
Replace it.
(2) The bulb burnt out very
often.
A wrong bulb is used.
Replace it with a correct one.
(3) The illumination is not
bright enough.
A wrong bulb is used.
Replace it with a correct one.
The use of light adjustment knob is
wrong.
Adjust correctly.