BestScope BS-2053 Series User manual

Biological Microscope
BS-2053, 2054 Series
Instruction Manual
BS-2053B BS-2054B
This manual is for BS-2053, 2054 series biological microscopes. To ensure the safety, obtain optimum performance
and to familiarize yourself fully with the use of this microscope, it is recommended strongly that you study this
manual thoroughly before operating the microscope.

BestScope International Limited
1
CONTENTS BS-2053, 2054
CONTENTS .........................................................................................................0
USER NOTICE......................................................................................................2
1. Components Name ........................................................................................3
2．Assembly......................................................................................................4
2.1 Assembly Diagram..................................................................................4
2.2 Assembly Procedure...............................................................................5
3. Adjustment and Operation.............................................................................8
3.1 Adjustment set diagram.........................................................................8
3.2 Adjustment and Operation...................................................................11
4．Technical Specifications..............................................................................17
5．Outfit .........................................................................................................18
6．Troubleshooting Guide...............................................................................19

BestScope International Limited
2
USER NOTICE BS-2053, 2054
Ⅰ. Safety Notice
1. Open the box carefully to avoid the accessories, like lens, dropping to ground or being damaged.
2. Do keep the instrument out of direct sunlight, high temperature or humidity, dusty and easy shaking
environment. Make sure the stage is flat, horizontal and firm enough.
3. When moving the microscope, carefully carry it with the handle and the base.
4. Make sure the instrument is earthed, to avoid lighting strike.
5. For safety, be sure the main switch is in “O” (off) state and cut off the power supply before replacing the bulb
or the fuse. If you replace the bulb during use or right after use, allow the lamp bulb and the lamp house to
cool completely before touching.
Designated bulb: 1W S-LED Lamp (BS-2053); 3W S-LED Lamp (BS-2054).
6. Check the input voltage: be sure the input voltage which is signed in the back of the microscope is consistent
with the power supply voltage, or it will bring a serious damage to the instrument.
7. Always use the power cord provided by We.
8. The electrical equipment of the microscope should be discarded as electronic waste.
II. Maintenance and Care
1. All the lenses have been adjusted properly; do not dismount them by yourself please.
2. The nosepiece and coarse and fine focusing parts are so delicate that it is forbidden to disassemble them
carelessly by yourself.
3. Keep the instrument clean, and do not pollute the optical element when wiping away the dust on the
instrument.
4. The contaminations on the prism, like fingerprints and oil smudges, could be gently wiped with a piece of soft
cloth or tissue paper, gauze which has been immersed in pure alcohol or ether. (Note that the alcohol and
ether are highly flammable, do keep them away from the fire or potential sources of electrical sparks, and
use them in a drafty room as possible as you can.)
5. Do not attempt to use organic solvents to clean the microscope components other than the glass
components. To clean them, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent.
6. When using, if the microscope is splashed by liquid, cut off the power at once, and wipe away the splash.
7. Do not disassemble any parts of the microscope, as this will affect the function or reduce the performance of
the microscope.
8. Place the instrument in a cool, dry position. When not using the microscope, keep it covered with a dust
cover. Make sure the lamp socket is cool before covering the microscope.
Ⅲ. Configuration Instruction
1. BS-2053 is the basic model and uses NIS45 system;
2. BS-2054 is the coding model, using NIS45 system, with brightness memory, adjustable color temperature
stage and other configurations.
IV. Safety Marks
The Following marks are on the microscope. Figure out the meaning of these symbols and always use the
microscope in the safest way.
Symbol
Meaning
Power on
Power off
Direction of light intensity. When turning to the tip, the intensity decreases from
strong to weak
SLEEP
Dormant State
LOCK
Light intensity is locked

BestScope International Limited
3
Eyepiece
Nosepiece
Condenser
Mechanical Stage
Main body
BS-2054
1. Components Name BS-2053, 2054
Viewing head
Nosepiece
Objective lens
Condenser
Mechanical Stage
Main body
BS-2053
Eyepiece
Viewing head
Objective lens

BestScope International Limited
4
10x Eyepiece
Power Adaptor
2．Assembly BS-2053, 2054
2.1 Assembly Diagram
Taking BS-2053 for example, the following figure shows the installation sequence of the components. The
number in the figure shows the assembly steps. The installation method of BS-2054 is the same as that of
BS-2053.
Before installing, be sure every components is clean, do not scratch any parts or glass surface.
Keep well with hexagon wrench provided. When changing the components, you will need it again.
Main body
Objective lens
①
②
③

BestScope International Limited
5
BS-2053, 2054
2.2 Assembly procedure
2.2.1 Installing the eyepiece (Fig1)
Insert the eyepiece into the eyepiece tube until they
are against each other, as shown in Fig1.
2.2.2 Installing the objective lens (Fig.2-4)
1. Adjusting the coarse focus knob until the support
device of the mechanical stage reaches its stop limit
position.
2. Screw the lowest magnification objective into the
nosepiece from the left or the right side, then
revolve the nosepiece clockwise and mount other
objectives by the sequence of low to high
magnification
Installing objective this way will make the change of
magnification to be easier during using.
Clean the objective regularly, for lens is susceptible
to dust.
When operating, use 10×magnification objective to
search and focus specimen firstly, then replace with
higher magnification objective if necessary.
When replacing the objective, slowly turn the
nosepiece until you hear “clicked”, that means the
objective is in place.
BS-2054 Model: Turn on the power supply. The
corresponding objective magnification on the LCD
screen will be highlighted (as shown in figure 4)
when the nosepiece moves to a certain position.
Then install the corresponding objective at this
location.
Fig.1
Fig.2
Fig.3
LOGO
10x
Fig.4

BestScope International Limited
6
BS-2053, 2054
2.2.3 Mounting the filters (Fig.5)
1. Open the condenser carriage①;
2. Place the required filter②into the condenser, then
close the condenser carriage.
★The filter of the standard outfit is green and baby
blue.
2.2.4 Connecting the Power Cord (Fig.6-7)
1. Set the main switch①“O” (off) state before
connecting the power cord.
2. Insert a section of the adapter plug② DC socket
connector ③of the microscope safely to ensure good
contact.
3. Insert the other end of the adapter plug into the
common power socket to ensure good contact.
★Do use the supplied adapter all the time. If lost or
damaged, select the same standard adapter, please.
★A wide range of voltage, like 100V ～240V, is
acceptable for this microscope.
②
①
Fig.5
①
②
Fig.6
③
Fig.7

BestScope International Limited
7
BS-2053, 2054
2.2.5 LED lamp replacement (Fig.8-10)
◎Generally, LED lamp is very durable, so it is not easy to
damage. If it is unfortunately damaged, please
purchase the LED (Fig.10) from your vendors.
1. Loosen the screw I of brightness adjustment knob to
take the brightness adjustment knob down (Ignore
this note for BS-2053 model). Loosen six screws of the
bottom plate to open the bottom plate①;
2. Loosen screw② to take the LED lamp③ down and
unplug the connector A from the breadboard. Mount
new LED lamp, tighten screw② and insert the
connector fully into the breadboard.
★Note: Be careful to take the bottom plate down
slowly in case that the wire inside the microscope is
pulled apart.
I
Fig.8
③
①
②
Fig.9
A
Fig.10

BestScope International Limited
8
Condenser focusing knob
Left Coarse Focus Knob
3. Adjustment and Operation BS-2053, 2054
3.1 Adjustment set diagram (Fig.11-13)
Aperture adjustment screw
Locking handwheel
Left Fine Focus Knob
Brightness Adjustment Knob
BS-2053
Fig.11

BestScope International Limited
9
Portrait Adjustment Knob
Lateral Adjustment Knob
Right Coarse Focus Knob
Tension Adjustment Collar
Power switch
BS-2053, 2054
Interpupillary Distance Indicator
Right Fine Focus Knob
BS-2054
Fig.12
2

BestScope International Limited
10
Condenser focusing knob
Locking handwheel
Left Coarse Focus Knob
N-128
LOGO
*The other side of BS-2054 is the same as BS-2054, so it is no longer expressed.
BS-2053, 2054
Aperture adjustment screw
Left Fine Focus Knob
Encode Knob
LOGO
Fig.13
2
The symbol is converted
to””when up lighting
Time adjustable, adjustive
division 1min
The number changes from 0
to 100, the change value is 5
for one grid
Solid blue dot
Number adjustable
“LOCK”means that the
light can not be adjusted.

BestScope International Limited
11
BS-2053, 2054
3.2 Operation
3.2.1 Brightness Adjustment (Fig.14-20)
1. Connect the power cord and set the main switch① to
“－”state (ON).
2. Turning the brightness adjustment knob ②.
◎Other operation of brightness adjustment knob for
BS-2054 model
3. Click Knob: enter the standby state and “SLEEP” appears
on the screen, as shown in figure 17. Click again to
eliminate the state, the “SLEEP” on the screen disappears
and the normal working state is displayed;
4. Long press the knob for 3s : choose to set sleep after
the fixed time (Fig18), and the value of minute starts to
beat. The value of hours tarts to beat after clicking the
knob. The time can be increased or reduced by turning
the knob. The increase or decrease of lattice value is 1
minute and the maximum value can be set to 8 hours.
After setting to the required time, the time number beats
three times and then stops beating, which meaning the
setting is successful. Time begins to decrease by minute;
5. Double-click Knob: lock brightness or unlock (Fig.19).
The brightness adjustment knob fails when locking,
and ”LOCK” appears on the LCD screen; Double-click the
knob again to release the lock and the “LOCK” on the
screen disappears;
★”LOCK” means that the user sets specific brightness
when using a certain magnification objective, and uses
the lock function to prevent it from being changed by
another user. (In this case, when switching to another
magnification objective,
★
①
Fig.14
②
Fig.15
③
Fig.16
LOGO
Fig.17
LOGO
Fig.18

BestScope International Limited
12
Fig. 21
②
③
①
BS-2053, 2054
The brightness automatically changes to the
brightness of the corresponding magnification, but
the brightness adjustment knob fails.
6. Long press the knob and open the Power: Set up
Objective Magnification. (Fig.20). All objective
magnification are showed in the LCD Screen, the first
number is jumpy. Rotating the knob and stop when the
magnification shows which is your request. If you want
to set up next magnification, please click knob. Long
press the knob mean all magnification are set up.
3.2.2 Placing the Specimen (Fig.21)
1. Place the specimen the center of the mechanical stage
and use the stage clips to clamp it.
2. Turn the portrait and lateral adjustment knob①of the
mechanical ruler, move the specimen to the required
position.
★Be careful when changing the objective. If you finish
the observation with the short working distance
objective, and want to change another one, be careful
of not letting the objective touch the specimen.
3.2.3 Focusing the Specimen (Fig.22)
1. Focus the specimen with 10X objective. To avoid the
objective touching the specimen during focusing, you
should raise the mechanical stage to let the specimen
close to the objective at first, then slowly separate
them to bring the specimen to focus. Turn the coarse
focus knob① conversely to lower the specimen and
search images in the 10×ocular simultaneously, and
then use the fine knob②to focus. After that, you can
replace with other magnification objectives safely, and
focus without the risk of damaging the specimen.
Fig. 19
Fig. 20
①
Fig.22

BestScope International Limited
13
To make the observation more convenient, you can
use the locking set③ to fix the stage in a vertical
direction.
3.2.4 Condenser Adjustment (Fig.23)
Turn the condenser focus knob①to move the
condenser up and down. Raise the condenser when
using the high magnification objective, and descend it
when using the low magnification one.
1.Focus the specimen with 10× objective.
2.Adjust the condenser focus knob① to get a clear
image of the field iris diaphragm.
3.Turn the condenser centering knobs② to center the
image of field iris diaphragm in the field of view.
The condenser and the objective are coaxial. It has
been adjusted before leaving factory, so the user
needn’t to adjust them by self
The highest position of the condenser has been
adjusted too. It also needn’t any user’s operation.
(The top surface of the condenser is 0.03mm-0.4mm
lower than the stage top surface.)
3.2.5 Aperture Iris Diaphragm Adjustment (Fig.24)
Turn the aperture iris diaphragm stick① to adjust the
aperture iris diaphragm.
The aperture iris diaphragm is designed for the
adjustment of the numerical aperture, not for the
brightness.
Generally, setting the aperture iris diaphragm to 70-
80% of the N.A. of the objective in use will provide an
image with good contrast. If you want to observe the
image of the aperture iris diaphragm, remove one
eyepiece and look through the tube. You will see a
dark circle encroaching on the bottom of the tube.
②
①
Fig.23
①
Fig.24
BS-2053, 2054

BestScope International Limited
14
BS-2053, 2054
Fig.25
3.2.6 Adjusting the Interpupillary Distance (Fig.25)
The interpupillary distance range:48mm～75mm。
While looking through the eyepieces, move both
eyepieces round until the left and right fields of view
coincide completely.
3.2.7 Adjusting the Diopter (Fig.26)
Turn the eyepiece①to adjust the diopter while
looking through it.
★ The diopter range of the eyepiece is ±5 diopter.
The number aligned to the line on the viewing head
is the diopter in use.
3.2.8 Adjusting the tension adjustment collar
(Fig.27)
Turn the tension adjustment collar① with your
fingers. When the collar is turned in the direction of
the arrow, the tension of the coarse adjustment knob
② increases. Turning the collar in the opposite
direction decreases the tension.
If the nosepiece descends on its own or if the
specimen gets out of focus quickly even when it is
brought into focus using the fine adjustment
knob③, it means the tension of the coarse
adjustment knob ② is too low. Turn the collar in the
direction of the arrow to increase the tension.
①
Fig.26
①
②
③
Fig.27

BestScope International Limited
15
○
4
Fig.29
BS-2053, 2054
4.1 Installing Trinocular Viewing Head And
Video Attachment (Fig.28)
1. Mount the trinocular viewing head according to the
assembly path① in Fig.28 into the round
dovetail of the microscope and tighten the screw
in A position to fix the viewing head;
2. Mount the video attachment according to the
assembly path ② into the connecting seat of
the trinocular viewing head and tighten the
screw in B position;
3. Mount the thread interface of CCD or CMOS
according to the path ③ into the video
attachment.
4.2 Focusing the Specimen
After obtaining clear image by binocular
observation, observe the image on the computer
or the monitor. If it is not clear, please turn the
focusing ring C on the video attachment until the
image is sharp enough.
◎The CCD/CMOS will not rotate following the
rotation of focusing ring Cto avoid the
entanglement of data line, which is more
convenient.
◎Binocular/Trinocular Switching Viewing Head
(Fig.29)
Select the required optical path with optical
path switching knob④
Sign
Eye: Camera (%)
100:0
0:100
B
A
①
②
Fig.28
CCD/CMOS
③
④
C

BestScope International Limited
16
①
②
Fig.32
BS-2053, 2054
5.1.1 Digital Viewing Head (Fig.30-31)
1.The digital observation head is connected to the
computer through the data line. Software
ScopeImage 9.0 is installed on the computer to
process images.
2.The digital viewing head ① is an HDMI interface,
and the other end can be connected to a computer
or mobile phone;② Connect the network cable, ③
is the mouse interface.
3.Install the digital viewing head into the circular
dovetail of the microscope along the path shown in
FIG. 31. Tighten the screw in ④ position to fix the
digital viewing head.
5.1.2 Installing a tablet (Fig.32-33)
Open the hole cover plate at the back of Digital
viewing head, screw the support rod sleeve into the
threaded mouth ① as shown in FIG. 32, and then
insert the plate attachment as shown in FIG. 33.
★Tablets are optional and only work with digital
viewing heads.
Fig.30
○
4
③
Fig.31
①
Fig.33

BestScope International Limited
17
4. Technical Specifications BS-2053, 2054
4.1 Main Specifications
4.2 Eyepiece and objectives
4.2.1 Infinite plan Objective
Magnification
Numerical
Aperture (NA)
Focal Length
(mm)
Focal distance
(mm)
Working Length
(mm)
Objective
4×
0.10
0.17
45
28
dry
10×
0.25
0.17
18
7.4
dry
40×
0.65
0.17
4.5
0.7
dry
100×
1.25
0.17
1.8
0.14
oil
4.2.2 Eyepiece
Eyepiece
Magnification
Focal Length
(mm)
Field of View (mm)
Wide field eyepiece
10×
25
Φ20
4.2.3 Total Magnification
Eyepiece
10×
10×
10×
10×
Objective
4×
10×
40×
100×
Total Magnification
40×
100×
400×
1000×
Optical System
Infinite optical system
Viewing Head
Binocular Head, Digital viewing head, Trinocular head 30°Inclined, Interpupillary Distance
48-75 mm
Eyepiece
WF 10X/20
Nosepiece
Medium five hole nosepiece/Encoding nosepiece
Objective
Infinite plan Objective 4×, 10×, 40×, 100×
Focusing
Coaxial Coarse and Fine Adjustment, Moving Range 20mm, Fine Division 0.002mm
Condenser
Abbe Condenser, NA 1.25
Stage
Synchronous belt platform 150×139mm, Moving Range 75×52mm
Illumination
S-LED(1W/3W)

BestScope International Limited
18
5．Configuration table BS-2053, 2054
Item
Specification
Quantity
BS-2053B
BS-2054B
Main Body
Main frame (ordinary type without LCD )
1
●
/
Main frame (digital type without LCD )
1
○
/
Main frame (charging digital type without LCD )
1
○
/
Main frame (ordinary type with LCD )
1
/
●
Main frame (digital type with LCD )
1
/
○
Synchronous belt platform
1
●
●
Condenser bracket
1
●
●
Additional
function
Brightness memory
1
/
●
Adjustable color temperature
1
/
●
LCD display
1
/
●
Viewing Head
Infinite system
Hinged binocular viewing head
1
●
●
Digital viewing head
1
○
○
Trinocular viewing Head
1
○
○
Tablet
computer
10.1 inch, 1920x1080 resolution ratio
1
○
○
Nosepiece
Middle 5-hole Nosepiece
1
●
/
Middle 5-hole encoding Nosepiece
1
/
●
Illumination
1W SLED
1
●
/
3W SLED
1
/
●
Green Filter
1
〇
〇
Eyepiece
10×Wide Field Plan Eyepiece
2
●
●
Objective
Infinite plan Objective 4×
1
●
●
Infinite plan Objective10×
1
●
●
Infinite plan Objective40×
1
●
●
Infinite plan Objective100×（Oil）
1
●
●
Note: “●” Standard Outfit, ○”optional, BS-2054 without charge model

BestScope International Limited
19
6．Troubleshooting Guide BS-2053, 2054
6.1 Optical System
TROUBLE
CAUSE
SOLUTION
1. The edge of the field of
view is dark or the
brightness is not uniform
The nosepiece is not in the located
position (objective and light path not
coaxial)
Locate the nosepiece properly where
it clicks
The surface of the lamp becomes black
Change a new lamp bulb
A lens (the objective, condenser, eyepiece
or collector) is dirty.
Clean it thoroughly
2. Dirt or dust is visible in
the field of view
Dirt/dust on the specimen
Replace with a clean specimen
Dirt/dust on the eyepieces
Clean them
3. Visibility is poor Image is
not sharp;
Contrast is poor;
Details are indistinct
Specimen is not covered
Add cover glass on it
The thickness of the cover glass is not
suitable
Use standard cover glass with
thickness of 0.17mm
Specimen is placed reversely
Turn it over
Dry objective has oil on it. (especially for
40X objectives)
Wipe the oil
A lens (the objective, condenser, eyepiece
or collector) is dirty.
Clean it
Immersion oil is not used with the 100x
objective
Use specified oil
Air bubbles existed in the immersion oil
Eliminate the bubble
The aperture iris diaphragm is stopped
down too far
Adjust the aperture iris diaphragm
properly
Dirt or dust on the eyepiece
Clean it
4. One side of image is
blurred
Condenser is not properly centered
Center the condenser with the
centering screw
The nosepiece is not properly engaged
Engage the nosepiece properly
The specimen is not clamped
Clamp it with stage clips
5. The brightness is not
enough
The aperture iris diaphragm is too small
Adjust it properly
The condenser is too low
Adjust it properly
A lens (the objective, condenser, eyepiece
or collector) is dirty.
Clean it