Optika Italy B-150D Series User manual

Model
B-150D-MRPL
B-150D-BRPL
B-150D Series
INSTRUCTION MANUAL
Ver. 2.1 2020

Page 2
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Overview 4
5.1 B-150D-MRPL 4
5.2 B-150D-BRPL 5
6. Unpacking 6
7. Assembling 6
7.1 B-150D-MRPL 6
7.2 B-150D-BRPL 7
7.3 Assembling procedure 8
7.4 Polarizing set (optional) 9
8. Use of the microscope 10
8.1 Light intensity adjustment 10
8.2 Coarse focus tension adjustment 10
8.3 Stage 10
8.4 Adjust the interpupillary distance 10
8.5 Diopter adjustment 11
8.6 Use of oil immersion objective 11
8.7 Aperture diaphragm 12
8.8 Use with rechargeable batteries 12
8.9 Use of the polarizer (optional) 12
9. Use of software and digital head 13
10. Maintenance 14
11. Troubleshooting 15
Equipment disposal 16
Summary

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility
to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the
subject.

Page 4
COARSE
FOCUS KNOB
FINE FOCUS KNOB
EYEPIECE
OBSERVATION
HEAD
MAIN SWITCH
/ INTENSITY
ADJUSTMENT DIAL
CONDENSER
STAGE
LED CHARGE
INDICATOR
NOSEPIECE
OBJECTIVES
TENSION
ADJUSTMENT
COLLAR
SLIDE HOLDER
X/Y MOVEMENT
KNOBS
5. Overview
5.1 B-150D-MRPL

Page 5
COARSE
FOCUS KNOB
FINE FOCUS KNOB
EYEPIECES
OBSERVATION
HEAD
MAIN SWITCH
/ INTENSITY
ADJUSTMENT DIAL
CONDENSER
STAGE
LED CHARGE
INDICATOR
NOSEPIECE
OBJECTIVES
TENSION
ADJUSTMENT
COLLAR
SLIDE HOLDER
X/Y MOVEMENT
KNOBS
5.2 B-150D-BRPL

Page 6
① Frame
② Monocular observation head
③ Eyepiece
④ Tension adjustment tool
⑤ Objectives (4X / 10X / 40X)
⑥ Dust cover
⑦ Green lter
⑧ Frosted lter
⑨ Power supply
⑩ USB cable
③
④
⑤
⑥
⑦⑧
①
②⑨
⑩
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other
residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
7. Assembling
Once opened the box, the microscope parts are the following:
7.1 B-150D-MRPL

Page 7
7.2 B-150D-BRPL
③
④
⑤
⑥⑦⑧
①
②
① Frame
② Binocular observation head
③ Eyepieces
④ Tension adjustment tool
⑤ Objectives (4X / 10X / 40X / 100X)
⑥ Dust cover
⑦ Green lter
⑧ Frosted lter
⑨ Power supply
⑩ USB cable
⑪Immersion oil
⑨
⑩
⑪

Page 8
FFig. 2ig. 2
FFig. 3ig. 3
FFig. 1ig. 1
①
7.3 Assembling procedure
1. Remove the dust cap from the microscope frame
and from the bottom of the observation head.
2. Insert the optical head above the stand and tight-
en the screw ①. (Fig. 1)
• Hold the head with one hand during the
locking in order to avoid that the head falls.
3. Insert one end of the USB cable into the connec-
tor on the back of the optical head and the other
end into one of the USB ports on your computer.
4. Insert both eyepieces into the tubes of the optical
head. (Fig. 2)
• For model B-150D-MRPL: only one eyepiece
is used as this model is monocular.
5. Insert the power supply jack in the socket placed
at the rear side of the microscope. (Fig. 3)

Page 9
FFig. 6ig. 6
③
FFig. 4ig. 4
①
FFig. 5ig. 5
②
7.4 Polarizing set (optional)
1. Place the polarizer on the light exit ①at the base
of the microscope. (Fig. 4)
2. Loosen the head xing knob ② and remove the
head from the microscope frame. (Fig. 5)
3. Insert the analyser into the hole inside the frame
③. (Fig. 6)
4. Put back the head into its original position and
lock the xing knob.

Page 10
FFig. 7ig. 7
①
FFig. 8ig. 8
②
FFig. 10ig. 10
④
FFig. 9ig. 9
③
8. Use of the microscope
8.1 Light intensity adjustment
Operate on the light intensity adjustment dial ①to
turn ON / OFF the microscope and to increase / de-
crease the illumination voltage. (Fig. 7)
8.2 Coarse focus tension adjustment
• Adjust the tension using the provided tool.
The coarse knob tension is pre-setted in the factory.
1. To modify the tension according to personal’s
needs, rotate the ring ② using the provided tool
(Fig. 8).
• Clockwise rotation increases the tension.
• If the tension is too loose, the stage could go
lower by itself or the focus easily lost after ne
adjustment. In this case, rotate the knob in order
to increase the tension.
8.3 Stage
Stage accepts standard slides 26 x 76 mm, thickness
1,2 mm with coverslide 0,17mm. (Fig. 9)
1. Open the spring arm of the slide holder ③ and
place the slide from the front on the stage.
2. Gently release the spring arm of the slide holder.
• A sudden release of the spring arm could
cause the falling of the slide.
8.4 Adjust the interpupillary distance
Only B-150D-BRPL
Hold the right and left parts of the observation head
using both hands and adjust the interpupillary dis-
tance by turning the two parts until one circle of light
can be seen. (Fig. 10)
• The graduation on the interpupillary distance in-
dicator ④, pointed by the spot “.” on the eyepiece
holder, shows the distance between the opera-
tor’s eyes.
The range of the interpupillary distance is 48- 75 mm.