BestScope BS-2094 Series User manual

BS-2094 Series
Inverted Biological Microscope
Instruction Manual
BS-2094A BS-2094AF
BS-2094B BS-2094BF
This instruction manual is intended for users of the Inverted Series Biological Microscope BS-2094. To ensure
safety use, perform the best performance and to familiarize yourself with the use of this microscope, please read
this manual carefully before operating this product.

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Contents
Before Using ……………………………………………………………………
1
Safety Symbol…………………………………………………………………
1
Safety Precautions ……………………………………………………………
1
Care and Maintenance…………………………………………………………
2
Chapter 1 Introduction …………………………………………………………
4
1.1 Technical Specifications …………………………………………………
4
1.1.1 Main Technical Specifications and Configuration……………………
4
1.1.2 Objective Parameters…………………………………………………
5
1.1.3 Electrical Parameters…………………………………………………
5
1.2 Components ………………………………………………………………
6
Chapter 2 Overview of Agencies ………………………………………………
8
Chapter 3 Regulation and Operation …………………………………………
12
3.1 Turning the Power on ……………………………………………………
12
3.2 Turning the Illumination switch on………………………………………
12
3.2.1 Manual Lighting Switch……………………………………………
12
3.2.2 ECO Switch (Optional functions) …………………………………
12
3.3 Adjusting the Illumination Brightness……………………………………
13
3.4 Specimen Placement and Stage Knob Adjustment………………………
13
3.4.1 Mechanical Stage……………………………………………………
13
3.4.2 Pallet…………………………………………………………………
14
3.4.3 Auxiliary Stage………………………………………………………
14
3.5 Adjusting the Interpupillary Distance………………………………………
14
3.6 Focusing on the Specimens ………………………………………………
15
3.6.1 Focus Knobs…………………………………………………………
15
3.6.2 Focus Knob Tight Adjustment ………………………………………
15
3.7 Adjusting the Diascopic Illumination Aperture Diaphragm………………
16
3.8 Switching the Objective……………………………………………………
16
3.9 Operation the Phase Contrast Microscope…………………………………
17
3.10 Operation the Emboss Contrast Microscope ……………………………
18
3.10.1 Condenser-side Emboss Contrast Slider……………………………
18
3.10.2 Eyepiece-tube-side Emboss Contrast Slider ………………………
18

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3.11 Operating the Episcopic Illumination Microscopy………………………………
19
3.11.1 Episcopic Illumination………………………………………………
19
3.11.2 Fluorescence Filter Cube Turret ……………………………………
19
3.11.3 Filter Cube Switching Unit …………………………………………
19
3.11.4 Fluorescence Filter Cube……………………………………………
20
3.11.5 Fluorescence LED Unit ……………………………………………
20
3.12 Operating the LCD ………………………………………………………
21
3.12.1 The status of LCD …………………………………………………
21
3.12.2 Operating the illumination switch ………………………………
21
Chapter 4 Assembly………………………………………………………………
22
4.1 Installation diagram ……………………………………………………
22
4.2 Installation Steps ………………………………………………………
23
4.2.1 Installing the Condenser ……………………………………………
23
4.2.2 Installing the Phase Contrast Slider …………………………………
23
4.2.3 Installing the Moving Ruler …………………………………………
23
4.2.4 Installing the Objective ………………………………………………
24
4.2.5 Installing the Eyepiece ………………………………………………
24
4.2.6 Installing the LED Light Box ………………………………………
24
4.2.7 Installing the Camera…………………………………………………
25
4.3 Assembly Episcopic Illumination Microscopy…………………………………
26
4.3.1 Installing the Filter Cube ……………………………………………
26
4.3.2 Installing the Fluorescence LED Unit ………………………………
26
4.3.3 Replacing the Fuse …………………………………………………
26
Chapter 5 Maintenance and Storage……………………………………………
27
5.1 Cleaning …………………………………………………………………
27
5.1.1 Cleaning the Lenses …………………………………………………
27
5.1.2 Cleaning Parts other than Lenses ……………………………………
27
5.1.3 Cleaning off Immersion Oil …………………………………………
28
5.1.4 Decontaminating this Product ………………………………………
28
5.2 Storage ……………………………………………………………………
28
Chapter 6 Troubleshooting ……………………………………………………
29
6.1 Optical System ……………………………………………………………
29
6.2 Mechanical Parts …………………………………………………………
29
6.3 electrical Parts………………………………………………………………
30

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Before Using BS-2094
Safety Symbol
Symbol
Description
The surface is hot and cannot be touched with bare hands.
Please read this manual carefully before using the product. Incorrect
usage can cause personal injury or property damage.
The main power switch is turned on.
The main power switch is turned off.
Adjustment direction of illumination.
Ground connection.
ECO
People go lights out switch.
Up and down light switch.
Light switch.
Through the CE certification.
Lever in, Bino: Trino=100: 0
Lever out, Bino: Trino=0: 100
Safety Precautions
1. Be careful when opening box, prevent lens glass to stick to fingerprint, perspiration other
factors influence observation, prevent lens and other attachment drop damaged.
2. Avoid placing the microscope in place with direct sunlight, high temperature or high
humidity, dust, and strong vibration. Ensure that the stage is flat, horizontal and strong
enough.
3. If a bacterial solution or water spatters spill on the stage, objective or observing tube,
immediately unplug the power cord. Then, wipe off any solution or water with to keep the
microscope dry. Otherwise, it could damage the instrument.
4. To avoid blocking the natural convection air for cooling, make sure the left, right, top, and
rear of the microscope are at least 10 centimeters away from the wall and other objects.

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5. Grounding the machine to avoid lightning strike.
6. To ensure safety, always make sure the main switch is in "O" (disconnecting) and cut off
the power while waiting for the LED lights and the lamp room to cool completely. Input
voltage check: the input voltage indicated on the back of the microscope is consistent with
the supply voltage, otherwise the microscope will be seriously damaged.
7. Use our company dedicated wire.
8. When carrying this product, one hand should firmly hold it by gripping the recess in the
front lower part of the main body and the other holds the recess in the rear of the main
body.
★Do not hold any other parts (such as the upper part of the illumination pillar, focus knobs, eyepiece tube,
or stage) when carrying the microscope. Doing so might result in dropping or failure of this microscope.
Care and Maintenance
1. All lenses have been calibrated, please do not disassemble by yourself.
2. The structure of objective converter and coarse and fine focus mechanism are precise,
please do not disassemble easily.
3. The instrument should be kept clean, have to regular removal of dust, special attention
should be paid to do not contaminate the optical parts when cleaning.
4. The stain of the lens, such as fingerprints and grease can be wiped with a small amount of
ethyl ether (70%) and alcohol (30%) mixed solution.
Recess in the rear
Recess in the front lower part
F
F

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5. Do not use organic solvents to clean the non-optical parts of the microscope. Please use
neutral detergents when cleaning.
6. When using, if the microscope be wet liquid, should immediately cut off the power and
dry.
7. Do not disassemble any part of the microscope, which will affect the function or reduce
the performance of the microscope.
8. The instrument should be placed indoor with cool and dry, covered the dust cover when
not in use. Cover up, must wait for the lamp room to cool down.
9. The microscope usage environmental requirements:
a) For indoor use;
b) The environment temperature range: 10 ℃~ 35 ℃;
c) Maximum relative humidity: 80% at 31 ℃, linear temperature down to 34 ℃is 70%,
60% at 37 ℃, 50% at 40 ℃.
10. Microscope to store and transport environment requirements:
a) the environment temperature range: - 40 ℃~ + 70 ℃
b) relative humidity range: 10% ~ 100%
c) atmospheric pressure range: 500 hpa ~ 1060 hpa
Because the solvent such as ether and alcohol are extremely flammable, do not operate
power switch of various electrical equipment when using, and keep away from open fire, please
ensure indoor ventilation.

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Chapter 1 Introduction BS-2094
1.1 Technical Specifications
1.1.1 Main technical specifications and configuration
Device
Technical Specifications
BS-2094A
BS-2094AF
BS-2094B
BS-2094BF
Eyepiece
Extra Wide Field Eyepiece 10×/22
●
●
●
●
Field Eyepiece 15×/16
○
○
○
○
Field Eyepiece 20×/12
○
○
○
○
Observation head
Seidentopf Viewing Head, Inclined at 45°, Interpupillary
48-75mm
●
●
●
●
Objective
Infinity plane objective 4×
●
○
●
○
Infinity plane objective 10×
○
○
○
○
Infinity plane objective 20×
○
○
○
○
Infinity plane objective40×
○
○
○
○
Infinity plane Phase contrast objective 4×
○
○
○
○
Infinity plane Phase contrast objective 10×
●
○
●
○
Infinity plane Phase contrast objective 20×
●
○
●
○
Infinity plane Phase contrast objective 40×
●
○
●
○
Infinity plane semi-apochromatic objective 4×
—
●
—
●
Infinity plane semi-apochromatic objective 10×
—
●
—
●
Infinity plane semi-apochromatic objective 20×
—
●
—
●
Infinity plane semi-apochromatic objective 40×
—
●
—
●
Nosepiece
Quintuple Nosepiece
●
●
—
—
Quintuple intelligent Nosepiece
—
—
●
●
Focusing mechanism
up 7mm, down 1.5mm, to limit up to 18.5mm
●
●
●
●
Centering telescope
lens tube φ30
●
○
●
○
stage
250mm×170mm
●
●
●
●
Moving stage
Y- 80mm, X- 128mm
●
●
●
●
Plain stage
Two plain stages
○
○
○
○
Condenser
NA=0.3, WD=75
●
●
●
●
illumination
Transmitted illumination: 3WLED
●
●
●
●
Fluorescent illumination: (B, G, U) 3WLED
—
●
—
●
Fluorescent illumination: V, 3WLED
○
○
○
○
Universal phase
contrast slider
4×, 10×/20×/40× (for phase contrast)
●
●
●
●
4×, 10×/20×, 40×, 60× (for semi-apochromatic contrast)
○
○
○
○
Emboss contrast slider
contrast slider
○
○
○
○
Camera mount
C-Mount 1×
●
●
●
●
C-Mount 0.5×
○
○
○
○
LCD
—
—
●
●
Note: ● Standard component, ○ Choose component,― Inapplicability

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BS-2094
Optional Objective
No.
Technical Specifications
1
Infinity plan semi-apochromatic objective (adjustable) 20×, 40×, 60×
2
Infinity plan semi-apochromatic phase contrast objective (adjustable) 20×, 40×, 60×
3
Infinity plan semi-apochromatic phase contrast objective 4×, 10×, 20×, 40×, 60×
1.1.2 Objective parameters
Type
BS-2094A/BS-2094B
BS-2094AF/BS-2094BF
Magnification
4×
10×
20×
40×
4×
10×
20×
40×
Numerical aperture(N.A)
0.10
0.25
0.40
0.60
0.13
0.30
0.45
0.60
Work distance (mm)
30
10.2
12
2.2
17
7.4
8
3.6
Cover-glass thickness
0.17
0.17
0.17
0.17
1.2
1.2
1.2
1.2
Conjugate distance(mm)
∞
∞
∞
∞
∞
∞
∞
∞
1.1.3 Electricity parameter
Item
BS-2094A/BS-2094B
BS-2094AF/BS-2094BF
1
Input voltage
AC100-240V; 50/60 Hz
2
Fuse Size
T500 mA; 250 V
3
LED light
3W S-LED
4
Fluorescence LED light (B light）
—
wavelength is 485nm at 3W
5
Fluorescence LED light (G light)
—
wavelength is 520nm at 3W
6
Fluorescence LED light (U light)
—
wavelength is 360nm at 3W

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BS-2094
1.2 Components
The microscope BS-2094A components (following diagrams), BS-2094B add LCD based on
BS-2094A.
BS-2094A
Eyepiece
Viewing head
Mainframe
Moving stage
Objective
Condenser
Universal holder
Nosepiece
Stage

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BS-2094
The microscope BS-2094BF components (following diagrams), BS-2094AF = BS-2094BF remove
LCD screen.
BS-2094BF
Eyepiece
Viewing head
Mainframe
Universal holder
Condenser
Fluorescence
illumination
Objective
Moving stage
Filter Cube turret
Stage
Nosepiece
LCD

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Chapter 2 Overview of the microscopes BS-2094
1
Condenser aperture adjustment knob
7
Fine focus knob
2
Contrast slider
8
Coarse focus knob
3
Upper lighting shading part (only FL)
9
Brightness adjusting knob
4
Stage
10
Up/ down illumination switch (Transmitted
illumination for biological model)
5
Power switch
11
Power indicator
6
Nosepiece
12
Camera C-mount
BS-2094AF
2
10
3
4
4
5
11
1
6
7
8
9
12

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BS-2094
1
Emboss contrast slider
9
Moving stage
2
Fluorescent baffle (only FL)
10
Universal bracket/ holder
3
Elastic adjustment knob
11
Condenser set screw
4
Bracket horizontal movement knob
5
Bracket vertical movement knob
6
Fuse
7
Outlet
8
Built-in fluorescent light source part
and fuse (only FL)
•
5
11
1
3
4
7
2
6
10
9
8
BS-2094AF

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BS-2094
1
Condenser aperture adjustment knob
9
Fine focus knob
2
Contrast slider
10
Coarse focus knob
3
Universal bracket/ holder set screw
11
intelligent adjuster (only BS-2094B series)
4
Stage bracket/ holder
12
Power indicator
5
Fluorescence cube turret (only FL)
13
Camera mount
6
Power switch
14
LCD (only BS-2094B series)
7
Turntable cover (only FL)
8
Nosepiece clamp screw
2
3
4
6
1
8
9
10
7
5
11
12
13
14
BS-2094BF

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BS-2094
1
Fluorescent baffle (only FL)
8
Built-in fluorescent light source and fuse (only FL)
2
Universal bracket/ holder
9
Nosepiece
3
Emboss contrast slider
10
Moving ruler (attachable mechanical stage)
4
Elastic adjustment knob
11
Upper lighting shading part (only FL)
5
Stage X-direction movement knob
12
Condenser set screw
6
Stage Y-direction movement knob
7
Outlet
6
12
4
5
7
8
1
3
9
11
2
10
BS-2094BF

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Chapter 3 Operation BS-2094
3.1 Turning on the Power
Turn on the power and turn on the main
switch which is at the back of the
microscope body to "-" (on).
3.2 Turning the Illumination on
3.2.1 Manual lighting switch (for
BS-2094A series)
Make sure the power indicator is on and
press the light switch.
3.2.2 ECO switch (Optional functions)
As shown in figure, the induction device
can sense objects within 0.5m in front of
it.
When someone is observing within 0.5m
in front of the microscope, the blue light
in the induction device is on, the light
source of the microscope will not be
turned off. When the person leaves and
without any other object within 0.5m in
front, the blue light is off automatically.
And after 15 minutes, the LED will be off.
Press the ECO function key to open the
lighting again.
Closing ECO: Pressing the ECO function
key until the blue light go out.
Recovering ECO: restart the power.
Illumination
switch
Power switch
Induction device
ECO function key

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BS-2094
3.3 Adjusting the Illumination Brightness
As shown in the direction of rotation, the light
source is enhanced, whereas the light source
weakened.
3.4 Specimen Placement and Stage Knob
Adjustment
Load the slide holder into a mechanical stage,
and the cover glass slides downward slowly
into the grooves of the slide plate.
Rotate the stage knob to place the sample to
the desired position.
CAUTION
Be careful when changing the objective. The
objective may collide with the specimen,
because of the short working distance of
specimen observation.
3.4.1 Moving stage
The Moving stage is mounted and used on
the plain stage. The specimen can be moved
in the X-axis and Y-axis directions by operating
the stage knobs.
A 128×80 mm microplate can be placed on
the Moving stage. Attaching the different
specimen holders enables you to observe
various types of specimens.
The upper is the vertical knob, and rotates in
the direction as shown in the figure, the stage
will move forward in the vertical direction.
The lower is the horizontal knob rotating in
the direction shown in the figure. The stage
will move to the left in the horizontal
direction.
X-axis
Y-axis

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BS-2094
3.4.2 Holders
There are a variety of holders to choose from.
NO.
Model
1
Terasaki microplate
2
Universal holder
3
54mm Petri dish holder/ Slide holder
4
60 mm Petri dish holder/ Slide holder
5
90 mm Petri dish holder
3.4.3 Auxiliary stage
Connect the auxiliary stage to the left
and right of the stage to expand the stage
width. After connecting the auxiliary
stage, the stage width is 300 mm.
3.5 Adjusting the Interpupillary Distance
By rotating the binocular part while
looking through the eyepieces, adjust the
distance between the eyepieces so that
the right and left view fields overlap to a
single image from eyepieces
Auxiliary stage

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BS-2094
3.6 Focusing on the Specimens
3.6.1 Focus knob
To adjust the focus, rotate the focus knobs
on the right and left sides of the microscope
to move the objective up and down.
The figure to the right illustrates the
relationship between the rotational direction
of the focus knobs and the vertical notion of
the objective.
Focus travel: from the focal point, up to 7mm
and down to 1.5mm, and the limit can be up
to 18.5mm. (As the right figure, spin out the
limit screw for about 4 mm trip to limit)
The traveling distance of the objective for
each knob is as follows:
Rotation of knob
Distance traveled
by objective
Fine focus knob: 1 rotation
0.2 mm
Coarse focus knob: 1 rotation
37.7 mm
CAUTION
Never attempt the following operations, as
they might result in product malfunction:
◆Rotating the left and right focus knobs in
opposite directions.
◆Rotating the coarse and fine focus knobs
past their limit.
3.6.2 Focus knob tight adjustment
The focus knob become tighter, when
rotating direction of the tightness
adjustment knob is shown as figure 8.
Otherwise become more relaxed.
Tightness adjustment knob
limit screw
Coarse focus knob
Fine focus knob

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BS-2094
3.7 Adjusting the Transmitted Illumination
Aperture Diaphragm
Insert a centering telescope in the eyepiece
tube, adjust the focal length, and view the
objective pupil plane (a bright circle) and
the aperture diaphragm image. Adjust the
aperture diaphragm so that the size of the
diaphragm image is 70-80% of the size of
the objective pupil plane.
Aperture diaphragm is designed for the
adjustment of numerical aperture, not the
dimmer. Typically, adjusting the aperture
diaphragm to 70% to 80% of the pupil of the
objective will result in an appropriate
contrast and a favorable image. The iris
aperture adjusting handle is marked with
the aperture of the diaphragm.
3.8 Nosepiece
Turn the nosepiece until it clicks in place,
stays in the specified position, can switch
the objective.
Before rotating the converter, check the
objective height to prevent collision with
specimens or stage.
Normally, the objective is mounted by
rotating the nosepiece clockwise (from
above the nosepiece) increasing
magnification in turn.
CAUTION
Be careful when changing the objective.
The objective may collide with the
specimen, because of the short working
distance of specimen observation.
Aperture diaphragm
open/ close lever
Aperture diaphragm image
Objective pupil plane
70-80%
100%

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BS-2094
3.9 Operation the Phase Contrast Microscope
Phase contrast microscopy requires phase contrast (PH) objectives and PH sliders.
If PH Slider is used, it is possible to center the annular diaphragm. Turn the centering screw of the
PH slider to merge the annular diaphragm image with the phase ring image in the objective.
Decentering the phase ring and the PH annular diaphragm
Basically, the phase ring and the PH annular diaphragm should be adjusted so that
they are concentric. However, slightly decentering them will produce a shadowing
effect, resulting in a three-dimensional image.
Annular diaphragm
Centering hold
(on the side)
Hollow position
Centering hold
(on the side)
Hexagonal
screwdriver
Centering screws
Hexagonal
screwdriver