BioChain AnaPrep 12 User manual

BioChain Institute Inc.
Automated Nucleic Acid Preparation System
AnaPrep 12
Catalog No.: Z1320001
Rev. Date: 19 Aug. 2013
Manual No. Z1001
Version: 1.14
For General Laboratory Use Only
Instruction Manual

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TABLE OF CONTENTS
Page #
INTRODUCTION.................................................................................................. 3
SAFETY INFORMATION....................................................................................... 4
1.0 INSTALLATION ............................................................................................ 8
1.1 Composition of the AnaPrep 12 System .................................................. 8
1.2 Contents of Reagent Kits......................................................................... 9
1.3 Operating Environment / Condition ...................................................... 10
1.4 Unpack the AnaPrep 12 System ............................................................ 12
1.5 Overall View............................................................................................ 15
2.0 GETTING STARTED .....................................................................................16
2.1 Turning on the power............................................................................ 17
2.2 Preparation ........................................................................................... 18
2.3 Extraction.............................................................................................. 19
2.4 After Extraction Operation.................................................................... 25
3.0 Cleaning & Maintaining the AnaPrep 12 Instrument ..................................26
4.0 Troubleshooting.........................................................................................27
5.0 Specifications ..............................................................................................29
APPENDIX .........................................................................................................30

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INTRODUCTION
Thank you for purchase of the AnaPrep 12 system which is a fully automated,
standalone robot that can purify nucleic acids within 30-45 minutes. With
advanced magnetic bead separation technology, it enables you to have high
quality extraction results. Moreover, the most user-friendly interface makes
users free from troublesome parameter settings and maintenance.
This guide contains important information regarding the safe use of the
AnaPrep 12 system. Please read this manual carefully before you start to run
the system at the first time, especially for Safety Information.
If there is any question about how to install or operate it, please contact our
certified distributors / agents or email to our technical support center
( techsu[email protected]om ).
Contact Info:
Company: BioChain Institute Inc.
Address: 39600 Eureka Dr.
Newark, CA 94560, USA
Tel: [phone]
Fax: [phone]
Mail: inf[email protected]om
AnaPrep Magnetic Bead Preparation Process

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SAFFETY INFORMATION
The meanings of safety precaution marks are as follows:
WARNING:
“WARNING”indicates a dangerous condition that may lead to death or
serious injury.
BIOHAZARD:
This symbol is used to indicate that certain precautions must be tken when
working with potentially infectious material.
CAUTION:
This symbol is used to indicate that non-compliance with instructions or
procedures may lead to physical injury or even death or could cause
damage to the instrument.
Important:
“Important”shows the important notes for usage, as well as prohibited
actions.
Note:
“Note”indicates the notes, procedures that should be obeyed and
supplementary information for use.
HOT SURFACE:
This symbol is used to label potentially hot instrument surfaces.
For your safety and that of others, follow the guidelines provided in the
following pages concerning the use of the AnaPrep 12 system.

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About Instrument
WARNING:
Ignoring the following notations may lead to fire or electric shock.
- In countries other than US and Canada, use a power cable that meets
your country’s standard, or contact your local distributor.
- Do not use the AnaPrep 12 system with voltage other than the voltage
specified on the device.
- Do not use the AnaPrep 12 system with a damaged power plug or a
loose socket.
- If there is dust on the prongs of the power plug or on the plug socket,
remove it with a dry cloth.
- When you disconnect the plug from the outlet, be sure to hold the
power plug itself. Do not pull the power cable.
- For maintenance, disconnect the power plug from the outlet.
- Do not touch the power plug when you hear the crash of thunder.
Do not pour any liquid on the AnaPrep 12 system.
Do not place any objects containing liquid on the AnaPrep 12 system.
Doing so may cause a device failure, fire, or electric shock.
In the event the device overheats, starts to smoke or smells strange,
immediately unplug the power cable.
CAUTION:
Never attempt to remodel the AnaPrep 12 system without the
manufacturer’s permission. Doing so may cause fire or electric shock.
Do not place or drop objects on the AnaPrep 12 system. Also refrain
from bumping or knocking it, as doing so may cause a failure or
malfunction of the AnaPrep 12 system.
If any liquid materials are left inside the device, wipe it up a soft paper
tissue, etc. Otherwise, the AnaPrep 12 system may be damaged.

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Repairs to the AnaPrep 12 system should only be performed by such
agencies as are specifically authorized by the BioChain Institute Inc.
Only original the BioChain Institute Inc. replacement parts should be
used.
If the equipment is used in a manner not specified by the manufacturer,
the protection provided by the equipment may be impaired.
About Reagent Kits
CAUTION:
When handling any of the kits, refer to the respective handbook.
Reagents in each kit should be handled by observing the safety
information and precautions regarding the kit.
Extraction should be performed in an appropriate laboratory or
workplace.
Note:
The kits are not supplied with the AnaPrep 12 system. Select the desired kit(s)
and order it (them) separately.

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About Samples
BIOHAZARD:
Always wear appropriate gloves, a mask, and safety goggles etc. when
handling any infectious samples.
About Infectious Wastes
When handling or disposing of infectious materials, follow the
laboratory guideline or the law regarding infectious waste to perform
proper incineration, fusion, sterilization, and/or disinfection.
When you use a third party to dispose of it, outsource this work an
operator licensed to handle medical waste subject to special control,
and give them the manifest of the medical waste at the same time.
BioChain Service Center
For technical problem and instrument maintenance please contact our
service center:
BioChain Institute Inc.
39600 Eureka Dr.
Newark, CA 94560, USA
1-510-783-8588
1-510-783-5386
tech[email protected]

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1Installation
1.1 Composition of the AnaPrep 12 system
Check that the following are included in the package. Contact your local
representative if you notice any missing part(s).
A
B
C
D
E
Q’ty
A. AnaPrep Instrument 1
B. Power Cord 1
C. Sample Tube Rack 1
Automated Nucleic Acids Preparation
Catalog No: Z1320001
Rev. Date: April 9, 2013
Manual Number: Z1001
Version: 1.13
For General Laboratory Use Only

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D. Barcode Reader 1
E. User’s Manual 1
Please make all the components are free from damages as soon as you
get the system. If any damage found, please contact your local
representative for the instant support.
Note:
BioChain’s global warranty does not cover the damages from the
transportation or improper operation.
1.2 Contents of Reagent Kits
A
B
A. Reagent Cartridge(s)
B. Reaction Chamber(s)
C. Tip Holder
D. Piercing
D
C
E
F
G
H

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E. Filtered tip
F. Sample tube
G. Elute tube
H. Pestle (Option for some Kit types)
Note:
-Reagent kits are separated purchase. Please contact your local
agents or representatives to get further info.
-The contents of reagent kits will be various. Refer to the handbook
of kits enclosed in reagent box for details.
1.3 Operating Environment / Condition
Use the AnaPrep 12 system in a location that meets the following
conditions:
The space for the allocation of the AnaPrep 12 system system must
be remained 5-10 cm from instrument to side walls.
A location where power can be provided.
A location where the temperature is 10 to 40°C, and humidity is 30
to 80%RH (non condensing)
A location that is flat and stable, with no vibration

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A location away from direct sunlight (Block the sunlight by closing
curtains or blinds as necessary.)
A location which is well-ventilated and not dusty
A location where the temperature does not go up and down
suddenly (Warming a cold room suddenly or moving the AnaPrep
12 system from a room with low temperature to a warm room may
cause condensation inside the device, resulting in abnormal
extraction.)
A location where the temperature and humidity are kept within
the specified range (far from water taps, water heaters, humidifiers,
air-conditioners and heaters)
A location far from objects which generate strong magnetic fields
(motors, transformers, TV, audio speakers, magnets, etc.) (Bringing
the AnaPrep 12 system close to any type of magnetic field may
cause a malfunction.)
Warning:
Do not use the AnaPrep 12 system to a location where it is wet or can be
splashed with water. It may cause a device failure, fire, or electric shock.
When relocating the AnaPrep 12 system, disconnect the plug from the
outlet first. If the power cable is damaged, this may cause a device failure,
fire, injury, or electric shock.
Caution:
Do not use the AnaPrep 12 system in an unstable place such as a slanted
surface or a place subject to vibrations. It may cause injury or device
failure.
Do not use the AnaPrep 12 system in direct sunlight or close to a heating
device. It may shorten the life of the AnaPrep 12 system, or cause a
trouble.

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Operating Conditions
Items
Conditions
Temperature (°C)
During operation
10 –40
During down time
0 –55
Humidity (RH)
During operation
30 –80
During down time
10 –80
Max. wet bulb temperature
(°C)
During operation
29 (non condensing)
During down time
29 (non condensing)
Temperature gradient (°C/hr)
12 or less (non condensing)
Humidity gradient (RH/day)
30 or less (non condensing)
Altitude, operating (m)
1600 or less
1.4 Unpack the AnaPrep 12 system
(1) Open the packing box and take out the instrument and related
accessories.
Important:
The AnaPrep 12 system has a weight of more than 45 kgs. It
should be lift and moved by two persons.
Hold the moving handler of the instrument from two sides to
move it out from the box.
Do not hold the plastic outer covering.
Do not hold the front panel.

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Do not hold the door.
Caution:
Improper handling of the movement of the AnaPrep 12 system
will lead to instrument damages.
For correct and safe use of AnaPrep 12s, install it in a location
that is close to electrical outlet and has enough space for
installation and operation of the mains switch.
Important:
Keep the shipping box and stuffing materials. They will be
needed again when transporting the AnaPrep 12 system.
(2) Connect Barcode Reader with the Instrument.

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(3) How to remove the fixing stuff inside the instrument following the
steps below,
i. Plug in the power cord to the instrument and connect to electric
outlet
ii. Turn the power switch on.
iii. Press Start button
iv. The piston module will going up, and you can release the fixing
stuff.

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1.5 Overall View
Front view with Sliding Door closed
Front view with Sliding Door opened
LCM Panel and Control Pad
LCD Screen
Sliding Door
Moving Handler
Operational Area
UV Lamp
Control Pad

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Turn on and off
UV Light
-LCM displays information of operation instruction guide and
program process status.
-Control pad is used for selection of functions and input of
program and test codes.
LCM
Control Pad
Number
buttons
Pause or
terminate the
program
Start the program
/ wake up from
idle mode
Confirmation
/ Next Step
Cancel
/ Previous Step

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1 x Barcode Reader
Port & 1 x USB Port
Back View
2 Getting Started
Biohazard:
Always wear appropriate gloves, a mask, and safety goggles during any
biohazardous operations in extraction process. Even when touching the
device after any operation with a biohazard risk, wear appropriate gloves
and a mask since the device may be contaminated.
Important:
Before starting extraction, put on appropriate gloves, a mask, and safety
goggles if required by the operation. In the operation from preparing
samples to extraction completion, be careful not to contaminate the
samples with sweat, saliva, etc.
Power Switch
Power Inlet

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2.1 Turning on the power
(1) Make sure that the power cable are connected securely to the the
AnaPrep 12 system.
(2) Connect the plug of the power cable to the outlet.
2.2 Preparation
The following preparations are required for extraction operation.
Items to be prepared
- Gloves
- Mask
- Safety Goggles
- Reagent Cartridge*
- Reaction Chamber*
- Tip Holder*
- Pestle (Option)*
- Filtered tip*
- Sample tube*
- Elute tube*

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- Piercing Pin
For the preparation of samples, refer to the handbook of each
kit.
*: Provided with the Reagent Kits.
2.3 Extraction
(1) Turn the power switch on and waiting for the LCM screen turn on
and shows “AnaPrep 12 System Stand-By”.
(2) Press the “Start” button
(The system will process self-testing, and then go to steady mode)
Note:
The system will block main functions before the completion of
self-testing process.
(3) Open the sliding door and remove the sample rack from the
instrument.

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(4) Load Reagent Cartridges, and all plastics disposables (Reaction
Chamber, Tip Holder, Piercing Pin, Filtered Tip and Pestle
(optionally supplied with some kit types)
Insert the cartridges
How to pull apart reagent cartridges
Slash open the dotted line with nail and snap it with a little bit force.