biochrom EZ Read 2000 User manual

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EZ Read 2000 Microplate Reader
User Manual
Biochrom Ltd
22 Cambridge Science Park
Cambridge
UK
CB4 0FJ
Tel.: [phone]

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Table of Contents
1. INTRODUCTION AND INTENDED USE......................................................................................... .4
1.1 WHAT TO EXPECT FROM THE USER’S MANUAL.......................................................................................... 4
1.2 INTRODUCTION................................................................................................................................... 4
2. INSTRUMENT USE ..................................................................................................................... 5
3. INSTRUMENT CONNECTION AND SOFTWARE INSTALLATION AND USE......................................... 6
3.1 INSTRUMENT CONNECTION ..................................................................................................................6
3.2 SOFTWARE INSTALLATION..................................................................................................................... 6
3.3 QUICK MEASUREMENT......................................................................................................................... 7
3.4 KINETIC MEASUREMENT ..................................................................................................................... 12
3.5 MULTI-WAVELENGTH MEASUREMENTS.................................................................................................16
3.6 WAVESCAN MEASUREMENTS .............................................................................................................. 20
3.7CALIBRATING THE INSTRUMENT ..........................................................................................................23
4. TECHNICAL INFORMATION ...................................................................................................... 23
5. GENERAL FEATURES ............................................................................................................... 23
5.1 MEASUREMENT MODES .....................................................................................................................23
5.2 INTEGRATED QUALITY CONTROL...........................................................................................................23
5.3 SCOPE OF SUPPLY.............................................................................................................................. 23
5.4 LIABILITY .........................................................................................................................................24
6. MAINTENANCE ...................................................................................................................... 24
6.1 APPROVED PARTS..............................................................................................................................24
6.2 CLEANING AND DISINFECTION ............................................................................................................. 24

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6.3 INSTRUMENT CLEANING .....................................................................................................................24
6.4ADDITIONAL MAINTENANCE TIPS .........................................................................................................25
7. WARRANTY AND RETURN TO BASE .......................................................................................... 25
7.1 WARRANTY TERMS AND CONDITIONS ...................................................................................................25
7.2 RETURNS TERMS AND CONDITIONS ......................................................................................................26
7.3 RETURNS PROCEDURE........................................................................................................................26
8. ORDERING INFORMATION AND ACCESSORIES .......................................................................... 28
9. CONTACT INFORMATION ........................................................................................................ 28
9.1TABLE 2CONTACT INFORMATION ........................................................................................................28
10. APPENDIX ............................................................................................................................ 29
10.1 HELPFUL HINTS FOR SUCCESSFUL MICROPLATE ASSAYS...........................................................................29
10.2 TROUBLESHOOTING AND FREQUENTLY ASKED QUESTIONS ...................................................................... 29
11. DECLARATION OF CONFORMITY ............................................................................................ 32

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1Introduction and Intended Use
1.1 What to expect from the user’s manual
Instrument use
Software installation
Quick start guides for operational use (to perform a quick measurement, kinetics and
multi-wavelength measurements).
A technical explanation of how the instrument operates
General features
Helpful hints for obtaining the best measurements using the instrument.
Maintenance
1.2 Introduction
What is a microplate reader?
A microplate reader is a used to measure the absorbance of liquid samples in a 96-welll plate.
What do microplate readers measure?
Microplate readers measure the amount of specific wavelengths of light that is absorbed by
molecules within a solution.
Why is this useful and how do microplate readers measure sample absorbance?
The amount of light absorbed by molecules within a sample is proportional to the sample
concentration as described by Beer-Lambert’s Law:
A = e c b
A= absorbance
e = molar absorptivity (L mol-1 cm-1)
c = molar concentration (mol dm-3)
b = pathlength (cm)

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It is important to note that when measuring absorbance in a microplate well, the pathlength is
determined by the volume. In a microplate assay, it is very important that all wells have the same
volume and that all solutions are dispensed with an accurate liquid handler like an 8-channel pipette.
Common applications of absorbance-based microplate readers are ELISA, total protein (like Bradford
or BCA) and cell proliferation assays.
2Instrument Use
The Biochrom EZ Read 2000 Microplate Reader is used for microplate-based applications requiring
endpoint or kinetic absorbance measurements in optically clear 96-well plates with a standard
SBS/ANSI footprint.
Only trained laboratory personnel should operate the Biochrom EZ Read 2000 Microplate Reader.
The Biochrom EZ Read 2000 Microplate Reader is intended for general laboratory and research use
only.