Biosan S-bt smart biotherm User manual

Bioswisstec AG Ebnatstrasse 65 8200 Schaffhausen Tel. +41 52 620 33 44 Fax. +41 52 620 33 45

Contents

1. Safety Precautions ..........................................................................................................................................4

2. General information .........................................................................................................................................5

3. Getting started .................................................................................................................................................6

4. Operation .........................................................................................................................................................7

5. Fault diagnostics............................................................................................................................................10

6. Specifications ................................................................................................................................................11

7. Maintenance and Repair ...............................................................................................................................12

8. Warranty and Claims .....................................................................................................................................13

9. EU Declaration of Conformity ........................................................................................................................14

1. Safety Precautions

The following symbols mean:

Caution! Make sure you have fully read and understood the present Manual before using the

equipment. Please pay special attention to sections marked by these symbols.

Caution! Biohazard. Incubator operations should comply criteria of biosafety in microbiological

and biomedical laboratories, according to working material biohazard level.

Caution! Incubator is equipped with an audible signal to draw attention to sensor data and

warnings. Please follow the information on display. Error and alarm descriptions can

be found in Fault Diagnostics section on page 8.

Caution! The incubator contains a source of UV radiation. Do not start the unit without the cover

of the UV recirculator. Exposure to UV light is harmful and can cause damage to

unprotected eyes and skin.

GENERAL SAFETY

Use only as specified in the Operating Manual provided.

Do not use the unit if it has visible mechanical damage.

Store and transport the unit at ambient temperatures between -20°C and +60°C and maximum relative

humidity of 80%.

After transportation or storage and before connecting the unit to the mains, keep it under room temperature

for 2-3 hrs.

Before using any cleaning or decontamination methods except those recommended by the manufacturer,

check with the manufacturer that the proposed method will not damage the equipment.

Do not make modifications to the design of the unit.

When handling hazardous substances, follow the instructions contained in the safety data sheets for the

individual substances that are used and observe the relevant accident prevention regulations.

Follow respective safety datasheet when installing and handling the CO2reservoir.

ELECTRICAL SAFETY

Connect only to electric circuit with voltage corresponding to that on the serial number label.

Do not plug the unit into an ungrounded mains outlet, and do not use an ungrounded extension lead.

Ensure that the switches and plug are easily accessible during use.

Disconnect the unit from the electric circuit before moving.

If liquid penetrates into the unit, disconnect it from the mains and have it checked by a repair and

maintenance technician.

Do not operate the unit in premises where condensation can form. Operating conditions of the unit are

defined in the Specifications section on page 11.

DURING OPERATIONS

Do not operate the unit in environments with aggressive or explosive chemical mixtures. Please contact

manufacturer for possible operation of the unit in specific atmospheres.

Do not operate the unit if it is faulty or has been installed incorrectly.

Do not use outside laboratory rooms.

BIOLOGICAL SAFETY

It is the user’s responsibility to carry out appropriate decontamination if hazardous material is spilt on or

penetrates into the equipment.

The user is responsible for the incubator decontamination before its decommissioning and utilization.

Bioswisstec AG Ebnatstrasse 65 8200 Schaffhausen Tel. +41 52 620 33 44 Fax. +41 52 620 33 45

2. General information

S-Bt Smart BioTherm is designed for work in the areas of cell biology (operations with animal cell cultures

and tissues), molecular biology (DNA/RNA reaction analysis, hybridization reactions), biotechnology (synthesis of

target proteins and other molecules) and immunology (synthesis of antibodies and other proteins of immune system).

Unit provides a six-sided heating: the heating elements are located on the walls and on the door, thus providing

excellent uniform temperature distribution, regardless of external factors, such as ambient temperature and

positioning of the device.

Built-in infrared CO2sensor allows accurate control of the CO2level. The sensor makes measurement non-

sensitive to changes in temperature and humidity inside the incubator.

The chamber is made of stainless steel with smoothed seams to minimize contamination and to facilitate

cleaning.

S-Bt is equipped with a UV air recirculation system —1 UV lamp and a fan are mounted behind the rear wall,

providing decontamination of the working volume.

A convenient access port is built in the wall of the incubator for easy output of wire sensors or devices' installed

inside. The access port is heated independently to prevent formation of condensate.

Unit is equipped with error tracing and alarm systems, which significantly lower potential risks during operation.

Unit is equipped with a "black box" system that records temperature, humidity and CO2levels as well as

statuses for door opening, UV lamp, fan and errors to the inner memory.

Bluetooth connection to PC is available.

Application areas:

Cell biology: operations with animal cell cultures and tissues

Molecular biology: DNA/RNA reaction analysis, hybridization reactions

Biotechnology: synthesis of target proteins and other molecules

Immunology: synthesis of antibodies and other proteins of immune system

Features:

Six-sided heating provides uniform distribution of the temperature inside the chamber

Infrared CO2sensor, non-sensitive to temperature and humidity changes

UV recirculation system for decontamination cycles

Bluetooth data transfer to PC

"Black box" parameter logging system

Error tracing and alarm system

Separately heated sealable port for chamber access for cables.

3. Getting started

3.1. Unpacking

Remove packing materials carefully and retain them for future shipment or storage of the unit.

Examine the unit carefully for any damage incurred during transit.

The warranty does not cover in-transit damage.

Caution! Due to the unit's weight, unpacking and installing is to be carried out by no less than

two persons.

3.2. Complete set. Package contents:

Standard set

- S-Bt Smart BioTherm Compact CO2Incubator.................................................................................1 pce

- Power cable ........................................................................................................................................ 1 pce

- Hydrophobic filter................................................................................................................................. 2 pcs

- Shelves................................................................................................................................................ 3 pcs

- Water pan with lid................................................................................................................................. 1 set

- Access port seal (foamed PE)........................................................................................................... 10 pcs

- Operating manual, Certificate ........................................................................................................... 1 copy

Optional accessories

- Bluetooth dongle and control/logging software for PC ................................................................on request

- Additional shelves .......................................................................................................................on request

- Incubator stacking device............................................................................................................on request

3.3. Setup:

- Position the incubator on a stable platform that can support the weight of the incubator and materials

inside the incubator. Ensure access to an appropriate mains outlet.

- Connect the power cable to the socket on the rear panel of the unit. Check that power switches and

power plug are easily accessible.

- Locate the unit away from doors and windows and heating and air conditioning ducts. Drafts or direct

sunshine can affect thermal processes and cause condensation.

3.4. Before starting operations for the first time.

- Attach the hydrophobic filter to inlets on the top side of the rear panel. Pay attention that “IN” marking

must be facing right side when viewing the incubator from the front.

- Use 75% alcohol solution or any other decontaminating agent to clean the inner surfaces of the incubator

and the water pan with lid.

- Switch on the recirculator for half an hour in an empty incubator to decontaminate air in the incubator. To

switch on the UV recirculator, see p. 4.9.2.1.

3.5. CO2 tank requirements and setup.

This incubator is designed for CO2gas only. Connecting any other gas can result in a hazardous condition.

The incubator works with food grade CO2or better rated. Do not use a tank with siphon tube, the incubator

can be damaged by liquid CO2.

A two-stage reductor is highly recommended. Follow gas supplier instructions on installing reductors and

valves. Gas inlet on the incubator is a 3/8” barb fitting. Secure the tube with appropriate clamps and check

all connections for leaks. Gas flow should be set at 6 l/min.

Bioswisstec AG Ebnatstrasse 65 8200 Schaffhausen Tel. +41 52 620 33 44 Fax. +41 52 620 33 45

4. Operation

4.1. Connect the incubator to a grounded mains outlet. Turn the power switch on the rear of the incubator to

position I(on).

4.2. After initialization, display (fig. 1) shows:

Top line: current date and time, relative humidity inside, UV lamp icon (when the lamp is on);

Middle left: temperature panel, current temperature in larger font, set temperature in smaller;

Middle right: CO2control panel, current percentage in larger font, set percentage in smaller;

Bottom line: Status bar, UV recirculator timer (when the lamp is on);

Bottom: six buttons, Menu, t°C , t°C , CO2, CO2, Lock.

4.3. Installing shelves. Open the front door and the inner glass door. Position the shelves according to your

specification.

4.4. Humidity delivery. Pull out the water pan located on the bottom of the inner camera and fill with distilled

water (~80% of volume). With the set temperature of +37°C, incubator reaches 90% of relative humidity in

8-12 hours.

Note. When operating the incubator at 90% of relative humidity for longer than 48 hours,

humidity sensor values can have a measurement error.

4.5. Using access port.In order to use the access port, unscrew the cap of the access port located on the right

side of the incubator. Insert necessary cable through the port and into the supplied PE seal. Install the seal

into the access port. The access port is individually heated to compensate for temperature loss and

condensate formation.

4.6. Setting temperature. Using t°C and t°C buttons, set the necessary temperature.

4.7. Setting CO2levels. Using CO2and CO2buttons, set the necessary level of CO2.

Attention! Please make sure that the CO2 reservoir is attached correctly, see p. 3.4.

Note. After reaching temperatures higher than 50°C, CO2level measurement accuracy does

not conform to specifications.

4.8. Locking the buttons. Lock button locks the use of all the buttons under the display until Lock button is

pressed again.

4.9. Incubator menu (fig. 2).

Menu button opens incubator’s menu. Use Down and Up buttons to navigate and Enter button to select.

While browsing in the menu, select the topmost Quit option in order to return to the previous level. Option

GetID shows the serial ID and name of the incubator.

4.9.1. Timer (fig. 3). In this submenu user can set UV decontamination timer and Auto UV timer.

4.9.1.1 UV Decontamination Timer. Timer parameters are set in HH:MM format. After setting the timer, UV lamp

automatically switches on, main working screen displays an UV indicator (fig. 4, top right). After set time

has passed, the UV lamp automatically switches off. A timer is seen on the main working screen (fig. 4,

bottom right). To manually switch the lamp off, select the Turn Off UV option from the submenu.

4.9.1.2 Auto UV Timer. Each time after closing the door, UV lamp automatically switches on. After time set under

this menu option has passed or the door was opened, the UV lamp automatically switches off.

Note. A turned on UV lamp can rise the temperature above the set point: the longer UV lamp

stays on, the faster temperature jumps above the set point. Use this feature at your

own discretion.

Attention! UV recirculation should be used for decontamination cycle while setting up and after

operation, see p. 7.3.

Note. In case of manual UV lamp switch off, timer continues working despite the UV lamp is

off.

4.9.2. Date & Time (fig. 5). To set date and time in this submenu, press Enter button to select the parameter,

then Down and Up buttons to change the value and Enter button to confirm changes.

4.9.3. Service (fig. 6). This submenu has available options Current Errors and Settings.

4.9.3.1 Current Errors (fig. 7).This option allows user to view and reset the list of active errors. See Fault

diagnostics section, p. 5.3, for a list of possible errors.

4.9.3.2 Settings (fig. 8). User can access the Alarm Settings and Auto Lock Delay options.

4.9.3.2.1 Alarm Settings (fig. 9). User can set the alarm settings based on values of temperature, CO2level, and

relative humidity. All alarms are working on the following principle, temperature taken as an example:

If Tact < Tthr.low or Tact > Tthr.high for tdelay minutes, then an alarm will be triggered.

Where Tact is the actual temperature inside the incubator, Tthr.low is the lower threshold limit value, Tthr.high is

the higher threshold limit value and tdelay is the time delay in minutes. Time delay is used in order to avoid

false triggering of the alarms.

CO2level and relative humidity alarms are operated using the same principle.

4.9.3.2.2 Auto Lock Delay (fig. 10). After being idle for several minutes, the incubator locks the buttons (except the

Lock button). This timer allows to change the idle time.

4.9.4. Active Alarms (fig. 11). This option allows user to view and reset the list of active alarms. See Fault

diagnostics section, p. 5.5., for a list of possible alarms.

4.9.5. Data Logger (fig. 12). Data logger features following options: View Plot T(t), View Plot CO2%(t), View Plot

RH%(t), Clear log, Logger on/off.

4.9.5.1 All View Plot options use following interface. Figure 13 shows the temperature plot in relation to time.

4.9.5.1.1 Use Left and Right buttons to move the plot along the x axis

4.9.5.1.2 Use Up and Down buttons to move the plot along the y axis.

4.9.5.1.3 Use <-xx-> button to choose the x axis navigational step. Available scales are 15 minutes, 1 hour, 1 day,

1 week and 1 month.

4.9.5.2 Clear Log button clears the logger’s data.

4.9.5.3 Logger On/Off button triggers the automatic logging of the data.

4.9.5.4 Memory write location indicator. When the data logger uses up all allocated memory, it begins overwriting

data from beginning. 100% of memory is used up in approximately 1 month.

4.10. After stabilization of temperature and CO2level, open the doors and load the materials in the incubator

according to methodological recommendations.

Note. If the door is left open for more than a minute, a short signal is sounded every 20

seconds until the door is closed.

4.11. Incubator air sampling. Sampling can be performed through the capped opening marked as “air sample”,

on the rear access panel.

4.12. To turn off the incubator, set the power switch into position О(off) and detach the mains plug from the

mains outlet.

4.13. Operation recommendations.

The incubator saves settings even when switched off.

During decontamination treat every visible surface and door. When working with potentially pathogenic

material, use more potent decontamination method. See p. 7.3.

Bioswisstec AG Ebnatstrasse 65 8200 Schaffhausen Tel. +41 52 620 33 44 Fax. +41 52 620 33 45

Fig. 1. Working screen Fig. 2. Menu Fig. 3. Timer menu

Fig. 4. UV recirculator is on Fig. 5. Date & Time menu Fig. 6. Service menu

Fig. 7. Current Errors menu Fig. 8. Settings menu Fig. 9. Alarm Settings menu

Fig. 10. Auto Lock Delay menu Fig. 11. Active Alarms menu Fig. 12. Data Logger

Fig. 13. Temperature plot

5. Fault diagnostics

5.1. The incubator is equipped with an alarm and error system. Active alarms and errors are appropriately

indicated on the display.

Alarms and errors are followed by sound signal that can be turned off by pressing any button.

5.2. Error text can be found in Menu > Service > Settings > Current Errors, see p. 4.9.4. Choose Errors

Reset and confirm to reset the error list.

5.3. Error list can be found in the table below.

CO2 Sens. Err.

СО2sensor error

Back

Back temperature sensor error

Bott.

Bottom temperature sensor error

Left

Left wall temperature sensor error

Right

Right wall temperature sensor error

Ceiling temperature sensor error

Door

Door temperature sensor error

Chmb.

Temperature sensor in the incubator error

Perimeter T.Sens. Err.

Perimeter temperature sensor error

Recirc. Lamp Err.

UV lamp error

Communication Err.

Error connecting to power board

CO2.Sens.Comm.Err.

Error connecting to CO2sensor board

5.4. Alarm text can be found in Menu > Active Alarms. Choose Alarms Reset and confirm to reset the alarm

list.

5.5. Alarms list can be found in the table below. Alarms are adjustable, see p. 4.9.3.2.1.

Temp. High Alarm

Adjustable limit is exceeded.

Temp. Low Alarm

CO2High Alarm

CO2Low Alarm

Low Humidity

UV Lamp Hours > 9000h

Lamp reaches end of life time. Consider a replacement.

Bioswisstec AG Ebnatstrasse 65 8200 Schaffhausen Tel. +41 52 620 33 44 Fax. +41 52 620 33 45

6. Specifications

The unit is designed for operation in cold rooms, incubators and closed laboratory rooms at ambient

temperature from +4°C to +40°C in a non-condensing atmosphere and maximum relative humidity 80% for

temperatures up to 31°C decreasing linearly to 50% relative humidity at 40°C.

6.1. Chamber Material ................................................................................................................. Stainless Steel

6.2. Temperature setting range ..................................................................................................+25°C ... +60°C

6.3. Temperature resolution ....................................................................................................................... 0.1°C

6.4. Temperature stability......................................................................................................................... ±0.1°C

6.5. Temperature uniformity @37°C ........................................................................................................ ±0.3°C

6.6. Working volume................................................................................................................................46 litres

6.7. Number of shelves ........................................................................................................ 6 max. (3 provided)

6.8. Outer door .................................................................................................................Heated, made of steel

6.9. Inner door ......................................................................Lockable glass with UV protection film, 4 mil clear

6.10. Relative humidity ................................................................................................................... >90% @ 37°C

6.11. Humidity delivery .................................................................................................... water pan by ventilation

6.12. CO2control range* ...............................................................................................................0 - 20% ±0.2%

6.13. CO2sensor ................................................................................................................... Infrared CO2sensor

6.14. Display............................................................................................................................................... TFT 5’’

6.15. Recirculation system .................................................................................... 1 × 6 W UV lamp (TUV G6T5)

6.16. Recirculation system radiation type .........................................................................................UV (254 nm)

6.17. Average UV lamp life time................................................................................................................. 9000 h

6.18. Data transfer.................................................................................................................Wireless (Bluetooth)

6.19. Internal data memory ........................................................................................1 month of nonstop logging

6.20. Access port...........................................................................................................1 (ø 26 mm with PE seal)

6.21. Dimensions (L×W×H) ...................................................................................................... 400×410×580 mm

6.22. Work area (each shelf) ............................................................................................................ 350х350 mm

6.23. Alarms .................................................. Over-heating/cooling, CO2leakage, Humidity loss, UV lamp error

6.24. Working voltage.................................................................................................................. 230 V, 50/60 Hz

6.25. Weight**............................................................................................................................................ 37.7 kg

Replacement parts Catalogue number

Hydrophobic filter with tubing BS-010425-S01

Water pan and lid BS-010425-S02

Access port seal, foamed PE BS-010425-S03

Optional accessories Catalogue number

Shelf BS-010425-AK

Bluetooth dongle and software for PC BS-010425-BK

Incubator stacking device BS-010425-CK

Biosan is committed to a continuous programme of improvement and reserves the right to alter design and

specifications of the equipment without additional notice.

* At temperatures below 50°C

** Accurate within ±10%.

7. Maintenance and Repair

7.1. If the unit requires maintenance, disconnect the unit from the mains and contact Biosan or your local Biosan

representative.

7.2. All maintenance and repair operations must be performed only by qualified and specially trained personnel.

7.3. Regular service schedule

7.3.1. After operation

Switch on the recirculator running for 1 to 3 hours in an empty incubator to decontaminate air in the

incubator. To switch on the UV recirculator, see p. 4.9.1.1. After decontamination, slightly open the

incubator doors –UV lamp has been known to produce reactive oxygen species in minor concentrations.

7.3.2. Monthly (more frequently, if necessary)

Rub the inner surfaces, water pan with lid and shelves of the incubator with 75% ethanol solution or other

acceptably disinfectant such as sodium hypochlorite solution, and allow them to dry.

After cleaning the glass door, rinse with water and dry the excess to avoid streaks and possible corrosion.

Remove dust with moist cloth piece from outer incubator surface, especially front screen and upper part of

the incubator.

Visually check the hydrophobic filter (p. 7.7.) and access port seal (p. 7.8.) and replace if necessary.

Check the accuracy of CO2sensor system by measuring the actual CO2concentration with a reference

CO2analyser.

7.4. Expendable materials

Name

Description

UV lamp

TUV 6W, T5, G6

Fuse

M 3.15 A

Hydrophobic filter

filter

Hydrophobic filter set

Filter and tubing

Access port seal

Made from foamed PE

7.5. Fuse replacement.

Disconnect incubator from the mains. Disconnect power plug from power socket. Open fuse holder. Check

and replace if necessary, 230 V –М 3.15 А (type М- time lag: Medium).

7.6. UV lamp replacement. Average lifetime of UV lamps supplied is 9000 h. Replacement is necessary after

lamp stops functioning or at the end of manufacturer specified lifetime. Only the persons who have

completed special training are allowed to perform lamps replacement. UV lamp operation lifetime is che-

cked and tracked by the incubator.

7.7. Hydrophobic filter replacement. The filter should be replaced when it becomes glazed with dirt or by

sucked liquid. Maximum time of use is 30 days. We recommend replacing the filter immediately after full

cycle of maintenance.

Close the CO2valve on the gas reservoir and turn off the unit. Remove the filter from the tubing. Insert a

new filter into the socket and tighten it. Pay attention that “IN” marking must be facing right side when

viewing the incubator from the front.

7.8. Access port seal replacement. To prevent possible contamination of access port seal (made from foamed

polyethylene) and to ensure complete sealing of the port, the seal should be replaced after 30 days from

the first operation.

7.9. Calibration. Recalibrate the unit after the date on calibration certificate. To complete the process, contact

Biosan or your local Biosan representative.