Teledyne Installation Guide User manual

i
CombiFlash®Rf
Installation Guide
For indoor use only
COPYRIGHT © 2002, 2004 by
Teledyne Isco, Inc.
4700 Superior St., Lincoln, Nebraska, U.S.A. 68504
Phone: [phone]
Toll Free: [phone]
FAX: [phone]
Revision A: August 15, 2007
Part #60-5233-385

iii
CombiFlash®Rf
Installation Guide
Table of Contents
1. Introduction
1.1 Documentation Overview . . . . . . . . . . . . . . . . . . . . . . 1-1
1.2 Product Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
1.3 Operating Overview . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
1.3.1 Multiple Control Possibilities . . . . . . . . . . . . . 1-3
1.3.2 File Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
1.4 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
1.5 Controls, Indicators, and Features . . . . . . . . . . . . . .1-5
1.6 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8
1.6.1 Hazard Severity Levels . . . . . . . . . . . . . . . . . .1-9
1.6.2 Hazard Symbols . . . . . . . . . . . . . . . . . . . . . . .1-10
1.7 For Additional Information . . . . . . . . . . . . . . . . . . .1-11
2. System Preparation
2.1 Unpacking the Unit . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
2.2 Instrument Location . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
2.3 Connect Power . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
2.4 Connect Solvent Lines . . . . . . . . . . . . . . . . . . . . . . . . 2-2
2.5 Connect Waste Lines . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
2.6 Optional External Gas . . . . . . . . . . . . . . . . . . . . . . . .2-9
2.7 Connect and Route Drain Lines . . . . . . . . . . . . . . . .2-9
2.8 Position the System . . . . . . . . . . . . . . . . . . . . . . . . . . 2-9
2.9 Install Collection Tube Racks . . . . . . . . . . . . . . . . .2-10
2.10 Turn on Power . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-12
2.11 Configure the System . . . . . . . . . . . . . . . . . . . . . . . . 2-12
2.11.1 Solvents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-12
2.11.2 General Settings . . . . . . . . . . . . . . . . . . . . . . . 2-13
2.11.3 Vapor Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-13
2.11.4 Automatically Print Report at End of Run . .2-14
2.11.5 Enable Run Length Extension . . . . . . . . . . . . 2-14
2.11.6 Set Default Tube Volumes . . . . . . . . . . . . . . .2-15
2.11.7 Set Date/Time . . . . . . . . . . . . . . . . . . . . . . . . .2-15
2.11.8 Default Run Units . . . . . . . . . . . . . . . . . . . . .2-16
2.11.9 Gradient Method . . . . . . . . . . . . . . . . . . . . . .2-16
2.12 Prime the Solvent Lines . . . . . . . . . . . . . . . . . . . . . . 2-17

CombiFlash® Rf Installation Guide
iv
3. Operation
3.1 Sample Preparation . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.1.1 Liquid Sample Injection . . . . . . . . . . . . . . . . . 3-1
3.1.2 Solid Samples . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.1.3 Preloading on Column . . . . . . . . . . . . . . . . . . 3-3
3.2 Loading a RediSep RfColumn . . . . . . . . . . . . . . . . . 3-3
3.3 Start a Default Method . . . . . . . . . . . . . . . . . . . . . . . 3-4
3.4 During the Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6
3.4.1 Run Control Buttons . . . . . . . . . . . . . . . . . . . . 3-7
4. Remote Interfaces
4.1 Network Configuration . . . . . . . . . . . . . . . . . . . . . . . 4-1
4.1.1 Network PC Access . . . . . . . . . . . . . . . . . . . . . 4-2
4.1.2 Network Printing . . . . . . . . . . . . . . . . . . . . . . 4-3
4.1.3 Automatically Print Report at End of Run . . 4-4
4.2 Direct Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4
4.2.1 Windows 2000 Settings . . . . . . . . . . . . . . . . . . 4-5
4.2.2 Windows XP Settings . . . . . . . . . . . . . . . . . . . 4-6
4.2.3 Windows Vista Settings . . . . . . . . . . . . . . . . . 4-8
4.2.4 Completing the Direct Connection . . . . . . . . 4-10
5. Maintenance
5.1 Preventive Maintenance . . . . . . . . . . . . . . . . . . . . . . 5-1
5.2 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
5.2.1 Collection Rack and Tray Cleaning . . . . . . . . 5-2
5.3 Tubing Inspection . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
5.4 Flow Cell Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
5.4.1 Post Separation . . . . . . . . . . . . . . . . . . . . . . . . 5-4
5.4.2 Quick Cleaning when Recommended . . . . . . . 5-5
5.4.3 Monthly Flow Cell Cleaning . . . . . . . . . . . . . . 5-9
5.5 Electrical Troubleshooting . . . . . . . . . . . . . . . . . . . 5-10

1-1
CombiFlash®Rf
Installation Guide
Section 1 Introduction
1.1 Documentation Overview
This Installation Guide provides:
• Safety Information
• Unpacking instructions
• Installation instructions, including placing the
instrument on a network and direct connections with a
Windows computer
• Certification and warranty information.
Once you are operating the CombiFlash Rf, you may refer to
the Help menu for operating instructions and further
assistance.
The CD that accompanies this installation guide contains this
guide in Adobe Acrobat PDF format and the PeakTrak®
On-line Help in PDF format.
1.2 Product Overview
The Teledyne Isco CombiFlash Rfflash chromatography
system gives you high-productivity automation,
programmable gradients, full-spectrum UV detection and
peak separation, and automatic detection of columns and
collection tube racks. Its small size makes it a great “personal
system” and well suited for operation within chemical hoods
and other limited spaces.
WARNING
Avoid hazardous practices! If you use this instrument in any
way not specified in this manual, the protection provided by
the instrument may be impaired; this may increase your risk
of injury.

CombiFlash® Rf Installation Guide
1-2
Figure 1-1 CombiFlash Rf
Control of the system is through a simple to use interface. This
interface can be accessed through the touch screen panel, or a
Windows computer via a direct or network Ethernet
connection. These options give you the flexibility to operate
and monitor the CombiFlash Rfsystem at the instrument,
from a computer in the lab, or from remote locations.
The CombiFlash Rfis optimized for use with Teledyne Isco’s
RediSep®Rfcolumns, which are pre-packed with a variety of
media. For example, the CombiFlash Rfcan purify samples
from 4 milligrams to greater than 33 grams using RediSep Rf
columns loaded with 4 to 330 grams of silica gel.
Applications include purification of organic compounds for
drug discovery, as well as research in agrochemicals,
petrochemicals, natural products, polymers, and catalysts.

Section 1 Introduction
1-3
1.3 Operating Overview
The CombiFlash Rfmay be controlled through its built-in
touch screen panel or by a Windows computer running
Microsoft Internet Explorer 6 or newer.
The CombiFlash Rfis equipped with the touch screen display
for local control. For Windows computer control, the
CombiFlash Rfmay be controlled by the computer in two
different ways using TCP/IP protocol—direct or network. A
direct connection uses a cross-over cable between Ethernet
ports on the CombiFlash Rfand computer. A network
connection allows control from a computer on an established
network.
Note
Teledyne Isco recommends that you obtain assistance from your
Information Technology department before attempting direct or
network connections to a Windows computer.
1.3.1 Multiple Control Possibilities
The system can be accessed from the built-in touch panel and
up to ten network computers. The touch panel shares control
with all connected computers. The system performs the most
recent command from any control input.
1.3.2 File Storage
To support operation from a variety of direct and network
connections, the software and all files are stored in the
CombiFlash Rfon an internal hard drive. This ensures that
your compound purification methods and run history files can
be viewed from any connection.

CombiFlash® Rf Installation Guide
1-4
1.4 Specifications
Table 1-1 Specifications 1
Overall Dimensions Height: 61 cm (24")
Width: 36 cm (14.1")
Depth: 43 cm (17")
Weight 24.5 kg (54 lbs)
Power Options Input voltage range from 100 to 240 VAC, 2.0
Amps maximum
Line Frequency 50/60 Hz
Ambient Temperature 20 to 40°C (maximum temperature must be
at least 15°C below the boiling point of the
solvent)
Humidity (when
connected to power)
95% relative humidity maximum at 20 to
40°C
Flow Rate Range 5 to 100 ml/min
Flow Rate Accuracy ±5%
Pressure Limit Up to 200 psi (column dependant)
For columns undetected by RFID, the
maximum pressure is limited to 50 psi.
Pressure Accuracy 5% of full scale
Peak Detection Modes Slope or threshold
UV Spectrum 200 to 360 nm
Wavelength Accuracy ±5 nm
Flow Cell Pathlength 0.1 mm, ±25%
Fraction Accuracy ±[2ml +(flow rate ÷60)]
Pollution Degree 2
Electrical Safety per EN 61010-1
Installation Category II
Maximum Altitude 2000 meters
Note 1. All specifications are preliminary and subject to change.

Section 1 Introduction
1-5
1.5 Controls, Indicators, and Features
Figures 1-2 through 1-4 illustrate the controls on the
CombiFlash Rf.
Figure 1-2 CombiFlash RfFeatures (Front)
1. Touch Panel LCD display – Large 10.5 inch display for
system monitoring and control.
2. USB Port – Convenient, front panel port that accepts USB
Flash memory drive. A Flash memory drive may be
inserted into this port for transferring files, importing and
exporting methods, and system software updates.
3. Adjustable Column Mount – The injection valve
assembly and upper column mount slide along this mount
so the system can accept a variety of column sizes.
4. On/Standby Switch – 1 = On, 0 = standby.
5. Fraction Collector Arm and Drop Former – The arm
and drop former move to deposit liquid in the collection
tubes.
34
1
5
6
7
8
9
10
2

CombiFlash® Rf Installation Guide
1-6
6. Racksand Collection Tubes – The racks hold the
fraction collection tubes. Racks include an RFID tag which
the system uses to read the rack type and collection tube
size.
7. Sample Injection Port – Luer-type fitting to accept the
sample though either a solid load sample cartridge (shown)
or a liquid injection using a syringe or similar device.
8. Injection Valve – This six-way valve is automatically
positioned by the CombiFlash Rfaccording to its current
mode of operation. Modes include column equilibration,
sample injection, elution, column flushing, valve cleaning,
and system purging.
9. Upper Column Mount – Secures the column inlet.
10.Lower Column Mount – Secures the column outlet.
11.Top Shelf – Allows storage of accessories and solvent
bottles.
12.Column Expansion Ports – These ports allow the system
to add column channels with an optional module. This
option is not available at the time of printing.
13.Upper Drain Tube – Liquids spilled on the top shelf are
carried away through this tube to a user-supplied
container.
14.Ethernet Port – An 8P8C jack for a network connection
using a standard CAT5 cable, or for a direct connection to a
computer using a cross-over cable.
15.RS-232 Port – Serial communication port to interface with
expansion modules.
16.USB Port – For control of peripheral equipment. This rear
panel port is primarily for service-related functions.
17.Cooling Fan – Cools the internal electronic assemblies.
18.Check Valves – Externally-mounted pump check valves to
simplify pump maintenance.
19.Solvent Level Sensing Outlet Ports – uses air or
user-supplied gas to measure the hydrostatic pressure of
the solvent above the weighted tubing outlet.
20.Solvent Inlet Ports – Accepts two A solvents and two B
solvents.

Section 1 Introduction
1-7
Figure 1-3 CombiFlash RfFeatures (Side)
Figure 1-4 CombiFlash RfFeatures (Back)
11
12
19 20
23
16
13
14
15
22
21
18
24
17

CombiFlash® Rf Installation Guide
1-8
21.Optional External Gas Port – An inlet for an internal
pump which supplies pressurized air or user-supplied gas
for solvent level sensing, waste container full detection,
and system/column purges. The external gas should be
regulated to 2–5 psig before connecting the source to this
port.
22.Mains Power – Connects the system to AC line voltage.
23.Waste Ports – From left to right:
•Waste Level Sense – The system uses air or
user-supplied gas to measure the hydrostatic
pressure of the waste above the level sense line end.
The system will suspend operation when this level is
too high.
•Diverter Valve Waste – The diverter valve on the
fraction collector arm sends fluid to this waste port.
•Inject Valve Waste – The injection valve is cleaned
after each run. The system directs fluids from this
automatic cleaning routine to this port.
24.Lower Drain Tube – This tube carries away liquids
spilled onto the rack area.
1.6 Safety
Before installing, operating, or maintaining this equipment, it
is imperative that all hazards and preventive measures are
fully understood. While specific hazards may vary according to
location and application, take heed in the following general
warnings:
WARNING
Liquids associated with this instrument may be classified as
carcinogenic, biohazard, flammable, or radioactive. Should
these liquids be used, it is highly recommended that this
application be accomplished in an isolated environment
designed for these types of materials in accordance with
federal, state, and local regulatory laws, and in compliance
with your company’s chemical/hygiene plan in the event of a
spill.

Section 1 Introduction
1-9
WARNING
Avoid hazardous practices! If you use this instrument in any
way not specified in this manual, the protection provided by
the instrument may be impaired.
WARNING
If you are using flammable solvents or chemicals with this
system, vapor concentration levels may exceed the maximum
exposure levels as recommended by OSHA Guide 1910.1000.
To reduce those levels to a safe exposure, Teledyne Isco
recommends that you place the system in a laboratory hood
designed for the purpose of ventilation. This hood should be
constructed and operated in accordance with federal state and
local regulations. In the event of a solvent or chemical spill,
your organization should have a plan to deal with these
mishaps. In all cases, use good laboratory practices and
standard safety procedures.
1.6.1 Hazard Severity Levels
This manual applies Hazard Severity Levels to the safety
alerts. These three levels are described in the sample alerts
below.
CAUTION
Cautions identify a potential hazard, which if not avoided, may
result in minor or moderate injury. This category can also warn you
of unsafe practices, or conditions that may cause property
damage.
WARNING
Warnings identify a potentially hazardous condition, which if
not avoided, could result in death or serious injury.
DANGER
DANGER – limited to the most extreme situations to identify
an imminent hazard, which if not avoided, will result in
death or serious injury.

CombiFlash® Rf Installation Guide
1-10
1.6.2 Hazard Symbols
The equipment and this manual use symbols used to warn of
hazards. The symbols are explained in Table 1-2.
Table 1-2 Hazard Symbols
Warnings and Cautions
The exclamation point within the triangle is a warning
sign alerting you of important instructions in the
instrument’s technical reference manual.
The lightning flash and arrowhead within the triangle is a
warning sign alerting you of “dangerous voltage” inside
the product.
The pinch point symbol warns you that your fingers or
hands will be seriously injured if you place them
between the moving parts of the mechanism near these
symbols.
Symboles de sécurité
Ce symbole signale l’existence d’instructions
importantes relatives au produit dans ce manuel.
Ce symbole signale la présence d’un danger
d’électrocution.
Risque de pincement. Ces symboles vous avertit que
les mains ou les doigts seront blessés sérieusement si
vous les mettez entre les éléments en mouvement du
mécanisme près de ces symboles
Warnungen und Vorsichtshinweise
Das Ausrufezeichen in Dreieck ist ein Warnzeichen, das
Sie darauf aufmerksam macht, daß wichtige
Anleitungen zu diesem Handbuch gehören.
Der gepfeilte Blitz im Dreieck ist ein Warnzeichen, das
Sei vor “gefährlichen Spannungen” im Inneren des
Produkts warnt.

Section 1 Introduction
1-11
1.7 For Additional Information
Technical assistance for the CombiFlash Rfcan be obtained
from:
Teledyne Isco, Inc.
4700 Superior St.
Lincoln NE 68504
Phone: [phone] or [phone]
Fax: [phone]
E-mail: [email protected]
Vorsicht Quetschgefahr! Dieses Symbol warnt vor einer
unmittelbar drohenden Verletzungsgefahr für Finger und
Hände, wenn diese zwischen die beweglichen Teile des
gekennzeichneten Gerätes geraten.
Advertencias y Precauciones
Esta señal le advierte sobre la importancia de las
instrucciones del manual que acompañan a este
producto.
Esta señal alerta sobre la presencia de alto voltaje en el
interior del producto.
Punto del machacamiento. Sus dedos o manos seriusly
serán dañados si usted los coloca entre las piezas
móviles cerca de estos símbolos.
Table 1-2 Hazard Symbols (Continued)

2-1
CombiFlash®Rf
Installation Guide
Section 2 System Preparation
This section provides instructions for unpacking and installing
the CombiFlash Rfsystem. To prepare the system for
operation, sequentially follow all instructions in sections 2.1
through 2.12.
2.1 Unpacking the Unit
The CombiFlash Rfis shipped in a single carton. Carefully
unpack the shipment and inspect the contents. If there is any
damage to the shipping carton or any components, contact the
shipping agent and Teledyne Isco (or its authorized
representative) immediately.
WARNING
If there is any evidence that the system has been damaged in
shipping, do not plug it into AC power. Contact Teledyne Isco
or its authorized representative for advice.
Compare the contents of the boxes with the enclosed packing
slip. If there are any shortages, contact Teledyne Isco
immediately.
2.2 Instrument Location
The CombiFlash Rfhas a relatively small footprint, requiring
about 1550 square centimeters (240 in2) of level bench space.
Ensure that the CombiFlash Rfhas at least 3 cm (1.25") of air
space behind it for ventilation. Additional space may be
required for solvent and waste containers.
Refer to Table 1-1 for environmental conditions and power
requirements.

CombiFlash® RfInstallation Guide
2-2
WARNING
The system is heavy. Use a two-person lift to prevent injury.
Before making any connections to the CombiFlash Rf, place
the system on the bench or in the fume hood where it will be
operated. Temporarily position the system so you can access
the back panel to complete the connections.
2.3 Connect Power
Ensure that the On/Standby switch below the touch screen
panel is in the Standby position. Then, use the supplied IEC
power cord to connect the CombiFlash Rfto mains power.
WARNING
Mains power must meet the voltage, frequency, and amperage
requirements listed on the serial number label.
2.4 Connect Solvent Lines
WARNING
Risk of fire ignited by electrostatic discharges. Never
substitute the black tubing on CombiFlash systems. The black
PTFE tubing (P/N 023-0503-06) is conductive. This tubing is
required to dissipate static electricity.
CAUTION
To prevent damage or premature wear to the pump and internal
valves, clean solvent should be used. The solvent should not
contain any dissolved solids.
The CombiFlash Rfhas four solvent inlet ports on the back
panel: two A solvents and two B solvents. The system requires
at least one A and one B solvent to form a binary gradient, and
assumes that the B solvent is stronger than A. The second set
of inputs allow you to form alternative binary gradients, or
change the A or B solvent mid-run.
Accessory package (P/N 60-5239-006) includes several pieces
to complete the solvent inlet connections.

Section 2 System Preparation
2-3
Figures 2-1 and 2-2 show the recommended connections when
the user-supplied solvent container has a 38 or 45 mm opening
(GL 38 or GL 45). Note that there are two different styles that
can ship with the system. One styles includes a metal plate
and larger opening on the cap (Figure 2-1), the other has no
plate and a smaller opening on the cap (Figure 2-2).
Figure 2-1 Recommended solvent connection, with plate
60-0923-017 Ferrule
60-0923-015
Compression Fitting Nut
60-5234-130
Label (1 of 4)
60-5235-042 38 mm Cap, or
291-0010-06 45 mm Cap
60-5233-399 38 mm Plate, or
60-5233-400 45 mm Plate
60-5234-129
Inlet Filter Assembly
(Place weighted filter at
bottom of container.)
To Solvent Level SensingTo Solvent Supply
Connection

CombiFlash® RfInstallation Guide
2-4
Figure 2-2 Recommended solvent connection, without plate
If the container does not have a GL 38 or GL 45 opening, omit
the use of the cap and plate. Ensure that the solvent and air
tubing are secured so that the weighted filter remains at the
bottom of the solvent source.
60-0923-017 Ferrule
60-0923-015
Compression Fitting Nut
60-5234-130
Label (1 of 4)
60-5235-050 38 mm Cap, or
60-5235-051 45 mm Cap
60-5234-129
Inlet Filter Assembly
(Place weighted filter at
bottom of container.)
To Solvent Level SensingTo Solvent Supply
Connection

Section 2 System Preparation
2-5
The Solvent Level Sensing air line connects to the back panel
with a compression fitting. This fitting must be airtight. To
connect tubing using the compression fittings:
1. Slide the nut and ferrule onto the tubing as shown in
Figure 2-1 or 2-2.
2. Fully insert the tubing into the threaded bulkhead fitting
on the back panel of the system.
3. Finger-tighten the nut onto the threaded bulkhead fitting.
This will seat the ferrule in the fitting.
Note
When using higher-density solvents such as dichloromethane
(DCM), place the solvent container level with or above the
CombiFlash Rf system. Placing solvent containers below the level
of the Rf can contribute to decreased flow due to the high vapor
pressure of DCM. This problem becomes more pronounced as the
ambient temperature increases.
2.5 Connect Waste Lines
WARNING
Risk of fire or equipment damage. Failure to connect Waste
Port tubing may allow organic solvents to pool in unsafe areas,
possibly creating dangerous levels of flammable vapors.
WARNING
Risk of fire ignited by electrostatic discharges. Never
substitute the black tubing on CombiFlash systems. The black
PTFE tubing (P/N 023-0503-06) is conductive. This tubing is
required to dissipate static electricity.
CAUTION
Elevated flammable vapor levels are possible. Ensure that the
waste container is adequately ventilated, preferably by placing it in
a fume hood.
The system has two waste outlets on the back panel: Diverter
Valve waste and Inject Valve waste. You can route waste to a
common collection container, or to individual containers.

CombiFlash® RfInstallation Guide
2-6
The Waste Level Sense uses air pressure to detect the liquid
level in the common waste container. If using individual
containers, use the waste level sense to detect the level of the
Diverter Valve waste container. Under normal operating
conditions it will fill faster than the Inject Valve waste
container. Empty both containers at the same time to prevent
overfilling the unmonitored container.
Accessory package (P/N 60-5239-006) includes parts to
complete the waste connections. Figure 2-3 shows the
recommended connections for user-supplied waste containers
with either a 38 or 45 mm opening (GL 38 or GL 45). Note that
there are two different styles that can ship with the system.
One styles includes a metal plate (Figure 2-3), the other has a
plastic plate with four small openings on the cap (Figure 2-4).
Refer to the steps in section 2.4 to connect the tubing to the
system using the compression fittings. Then, complete the
connections according to Figure 2-3 or 2-4.
If your connection hardware matches Figure 2-3, connect the
Waste Level Sense line to the compression fitting in the center
of the cap. Push the ends of the two black waste lines through
the holes in the cap.
If your connection hardware matches Figure 2-4, push the
three tubes through the cap. Push the stop on the Waste Level
Sense tubing against the cap. This ensures that the Waste
Level Sense tubing is far enough into the container to detect
when it is nearly full.
Should the container not have a GL 38 or GL 45 opening, omit
the use of the waste cap. Ensure the solvent tubes are secured
so they drain into the waste container. Secure the Waste Level
Sense air tubing so its outlet is at least two inches (5 cm)
below the container’s maximum level.
Note
When using higher-density solvents such as dichloromethane
(DCM), ensure that the waste container is no more than 3 feet
below the supply solvent levels. Placing solvent containers above
the system and waste containers on the floor may cause the
internal check valves to open and allow solvent to flow through the
system when in the standby state.