Biogents BG-GAT User manual

Instruction Manual
Status: 2017/03 BG-GAT
Mosquito Control

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EN
Table of Contents
Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Product Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Assembling the Trap . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Removal of the Mosquitoes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Positioning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Technical Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Contact . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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The BG-GAT trap was developed by Dr. A.E. Eiras from the Universidade Federal de Minas Gerais, Belo Horizonte,
Brazil and Prof. Scott Ritchie from the James Cook University, Cairns, Queensland 4870, Australia.
Publications:
Eiras, A.E., Buhagiar, T.S., and Ritchie, S.A. (2014): Development of the Gravid Aedes Trap for the capture of adult female
container-exploiting mosquitoes (Diptera: Culicidae). J. Med. Entomol. 51(1): 200-209.
Ritchie, S.A., Buhagiar, T.S., Townsend, M., Homann, A., Van den Hurk, A.F., McMahon, J., and Eiras, A.E. (2014): Field validati-
on of the Gravid Aedes Trap (GAT) for collection of Aedes aegypti (Diptera: Culicidae). J. Med. Entomol. 51(1): 210-219.

3EN
Female mosquitoes are attracted by water and oviposition
cues and enter the transparent chamber through the fun-
nel. Once in the chamber, mosquitoes try to escape through
the translucent windows where they are exposed to a sti-
cky surface, oil, or insecticides. The catch bag provides a
barrier between mosquitoes and the infused water as well
as retains dead mosquitoes.
We recommend three methods to kill the mosquitoes:
1) Place a sticky card into the transparent chamber1. The
mosquitoes will stick to the card when ying around.
2) A thin lm of cooking oil can be spread on the inside of
the translucent chamber. The oil “wets“ the wings of the
mosquitoes, making ight impossible.
We recommend a neutral oil like canola and vegetable oil.
You can also use the aerosolised versions of these oils.
Avoid using light or avored oils such as olive oil, sesame
oil, and walnut oil as they may inhibit entry 1.
3) Alternatively you can use residual surface spray insecti-
cides that will kill mosquitoes through contact (for example
Mortein Outdoor Barrier Surface Spray, imiprothrin 0.3 g/
kg and 0.6 g/kg deltamethrin Reckitt Bensckiser Pty. Ltd.).
Do not spray the black bucket or entry funnel. This will re-
pel mosquitoes!
Retreat the translucent chamber monthly.
Function
Cross-sectional view
1 Heringer et al. (2016): Evaluation of alternative killing agents for Aedes aegypti in the Gravid Aedes Trap (GAT). Journal of
Medical Entomology. 0 (0): 1-7.

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1. Catch bag
2. Ring
3. Funnel
4. Transparent chamber
5. Black bucket with drainage holes
6. Sticky card holder
Product Components
1
4
2
5
3
7
6
Following component may be purchased separately:
7. Sticky cards (20 sticky cards per package)

5EN
Assembling the Trap
1. Unpack all parts and check if everything is there.
2. Fill the black bucket with water up to the
drainage holes (about 3 liters). Insert hay infusion (for
example 10 g of dry hay2 [F1]) or other organic materials
(for example 1 g of rabbit chow per liter or 1 g of alfalfa
pellets per liter) as oviposition attractants.
3. Mosquitoes will lay eggs through the mesh. Therefore, it
is a good idea to prevent larval development, e.g. with Bti
or an insect growth regulator such as methoprene pellets
in the water.
F1
Please note:
The smell of the new plastic of the traps might have repellent eects on the mosquitoes. Therefore, we recommend
to store the traps with some water outside for two weeks before using them in the eld to get rid of the new smell.
2 Mackay A. J., Amador M., Barrera R. (2013): An improved autocidal gravid ovitrap for the control and surveillance of Aedes
aegypti. Parasites & Vectors. 6:225.

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EN
4. Put the catch bag over the open end of the transparent
chamber [F2]. Tighten the top part of the catch bag by
pulling the cord of the bag tightly [F3 - F4]. You should at-
tach the catch bag with the seam facing outwards in order
to avoid mosquitoes getting stuck in the seam.
F2
F3
F4
F7
5. After strong or frequent rain the catch bag might hang
into the water due to its own weight making the identi-
cation of the captured mosquitoes even more dicult.
If you cannot avoid placing the trap where it is subject
to rain, we recommend xing the catch bag in place
with the mounting ring that is delivered with the trap:
Start by laying the ring over the net on one side [F5]. Then
press the ring equally on all sides down over the chamber
[F6] until the ring ts snuggly over the edge of the trans-
parent chamber [F7]. F5 F6
F7

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6. Place the transparent chamber on the black, water-lled
trap bucket [F8].
7. Adding preferred killing methods
Sticky cards:
To kill the mosquitoes, you can use sticky cards that
must be purchased separately. The mosquitoes will
stick to the dry adhesive on the card when ying
around in the transparent chamber.
F8
• You can place the card in the transparent chamber
with the help of the sticky card holder:
• Pop out the white Biogents disc from the hole
in the transparent chamber [F9].
F9

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F10
Narrow side
Wall
F13
• Place the sticky card holder into the hole [F10].
• If you need to lay the card on a ground for iden-
tifying the mosquitoes afterwards, pull o only
one side of the plastic lm from the sticky card
[F11]. Otherwise pull o both sides of the plas-
tic lm from the sticky card.
• Hang the sticky card with the pre-cut hole on
the hanger [F12]. Please hang the card with the
narrow side facing towards the wall [F13].
F12
F11

9EN
• Alternatively, just place the sticky card vertically
in the transparent chamber [F14] without hanging
it on the holder.
• Finally, insert the black funnel in the chamber [F15]
and rotate the funnel clockwise to secure it [F16].
F14
F16
F15

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F18
Cooking oil3
A thin lm of cooking oil can be wiped on the inside of the
translucent chamber [17]. The oil “wets“ the wings of the
mosquitoes, making ight impossible.
We recommend a neutral oil like canola and vegetable oil.
Alternatively you can use the aerosolised versions of these
oils.
Avoid using light or avored oils such as olive oil, sesame
oil and walnut oil as they may inhibit entry.
Residual surface insecticides
A killing agent (e.g., residual surface spray insecticide)
can be applied to the inner wall of the translucent cham-
ber [F18] to kill mosquitoes through contact (for example
Mortein Outdoor Barrier Surface Spray, imiprothrin 0.3 g/
kg and 0.6 g/kg deltamethrin Reckitt Bensckiser Pty. Ltd.).
Do not spray the black bucket or entry funnel. This will re-
pel mosquitoes!
Alternative killing methods
F17
3 Heringer et al. (2016): Evaluation of alternative killing agents for Aedes aegypti in the Gravid Aedes Trap (GAT). Journal of
Medical Entomology. 0 (0): 1-7.

11EN
Maintenance
Change the water (hay infusion or water with other attrac-
tants) once a month.
Remove any debris that falls into the trap or deters the mos-
quitoes from entering the trap.
If using sticky cards, replace the card at the latest when it is
full.
If using oil or surface spray, retreat the translucent chamber
monthly.
Removal of the Mosquitoes
Identication is complicated if the insects stay in the trap for
more than a week and dry out or if they get wet.
Removal of the mosquitoes:
Remove the funnel.
Loosen the catch bag and remove it from the transparent
chamber. Mosquitoes can then be either removed with
forceps, poured into a white tray for sorting, or the catch bag
can be closed by pulling the cord of the catch bag tightly
shut [F19]. In this way, the caught mosquitoes can be retur-
ned to the lab and identied.
Put a new catch bag over the open end of the transparent
chamber as described in F2 - F7.
When using sticky cards, remove the sticky card and replace
it with a new one as described in F10 - F14.
When using oil or a residual surface spray, check also the
inner wall of the transparent chamber for mosquitoes that
might stick to it.
Positioning
If you don‘t nd a naturally sheltered position for the trap, it would be best to position the trap under a roofed area, or a self
made shelter to keep it out of direct rainfall.
Even though rainfall does not damage the trap, there are some negative eects of rain:
• The mosquitoes can be damaged and dicult to identify.
• Mosquitoes avoid rain and prefer sheltered positions. In sheltered positions the catch rate can therefore be higher.
The black color of the BG-GAT attracts mosquitoes from some distance. So, the trap needs to be placed, where it is readily
visible but protected from rain. Please do not hide the BG-GAT in the bushes!
F19
Weight: 0.6 kg
Dimension: 27 x 38.5 cm
Technical Data

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Biogents AG
Weißenburgstr. 22
93055 Regensburg
Germany
www.biogents.com
E-mail: [email protected]om
Contact
Further information about the BG-GAT can be found at our webpage www.biogents.com
Mosquito Control