Biogents BG-GAT User manual

Instruction manual
Status: 2016/08 BG-GAT
Mosquito Control

2
EN
Table of Contents
Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Product Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Assembling the Trap . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Removal of the Mosquitoes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Positioning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Technical Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Contact . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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The BG-GAT trap was developed by Dr. A.E. Eiras from the Universidade Federal de Minas Gerais, Belo Horizonte,
Brazil and Prof. Scott Ritchie from the James Cook University, Cairns, Queensland 4870, Australia.
Publications:
Eiras, A.E., Buhagiar, T.S., and Ritchie, S.A. (2014): Development of the Gravid Aedes Trap for the capture of adult female
container-exploiting mosquitoes (Diptera: Culicidae). J. Med. Entomol. 51(1): 200-209.
Ritchie, S.A., Buhagiar, T.S., Townsend, M., Homann, A., Van den Hurk, A.F., McMahon, J., and Eiras, A.E. (2014): Field validati-
on of the Gravid Aedes Trap (GAT) for collection of Aedes aegypti (Diptera: Culicidae). J. Med. Entomol. 51(1): 210-219.

3EN
Female mosquitoes are attracted by water and oviposition
cues and enter the transparent chamber through the funnel.
Once in the chamber mosquitoes try to escape through the
translucent windows where they are exposed to oil/insecti-
cide. The catch bag provides a barrier between mosquitoes
and the infused water as well as retains dead mosquitoes.
To kill the mosquitoes, a killing agent (e.g., canola oil or
a residual surface spray insecticide) can be applied to the
inner wall of the translucent chamber:
1) A thin lm of cooking oil can be wiped on the inside of
the translucent chamber. The oil “wets“ the wings of the
mosquitoes, making ight impossible.
We recommend a neutral oil like canola and vegetable oil.
You can also use the aerosolised versions of these oils. Avo-
id using light or avored oils such as olive oil, sesame oil
and walnut oil as they may inhibit entry 1.
2) Alternatively you can use residual surface spray insecti-
cides that will kill mosquitoes through contact (for example
Mortein Outdoor Barrier Surface Spray, imiprothrin 0.3 g/
kg and 0.6 g/kg deltamethrin Reckitt Bensckiser Pty. Ltd.).
3) Another option is to place a sticky card into the transpa-
rent chamber 1.
Do not spray the black bucket or entry funnel. This will re-
pel mosquitoes!
Retreat the translucent chamber monthly.
Function
Cross-sectional view
1 Heringer et al. (2016): Evaluation of alternative killing agents for Aedes aegypti in the Gravid Aedes Trap (GAT), Journal of
Medical Entomology

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EN
1. Catch bag
2. Ring
3. Funnel
4. Transparent chamber
5. Black bucket with drainage holes
Product Components
1
4
2
5
3

5EN
Assembling the Trap
1. Unpack all parts and check if everything is there.
2. Fill the black bucket with water up to the
drainage holes (about 3 liters). Insert hay infusion (for
example 10 g of dry hay2[F1]), or other organic materials
(for example 1 g of rabbit chow per liter or 10 mg of
alfalfa pellets per liter) as oviposition attractants. If you
want to add additional lures like the BG-Lure, pop out
the white Biogents disc from the hole in the transparent
F1 F2
Please note:
The smell of the new plastic of the traps might have repellent eects on the mosquitoes. Therefore, we recommend to
store the traps with some water outside for two weeks before using them in the eld to get rid of the smell.
2 Mackay A. J., Amador M., Barrera R. (2013): An improved autocidal gravid ovitrap for the control and surveillance of Aedes
aegypti, Parasites & Vectors. 6:225.
F3 F4
Option 1: Cooking oil1
A thin lm of cooking oil can be wiped on the inside of the
translucent chamber [F3]. The oil “wets“ the wings of the
mosquitoes, making ight impossible.
We recommend a neutral oil like canola and vegetable oil.
Alternatively you can use the aerosolised versions of these
oils.
Avoid using light or avored oils such as olive oil, sesame
oil and walnut oil as they may inhibit entry.
chamber [F2] and place the BG-Lure cartridge into the
hole. If you don‘t use the BG-Lure anymore, make sure
that the hole is plugged so that the mosquitoes cannot
escape.
Mosquitoes will lay eggs through the mesh. Therefore it
is a good idea to prevent larval development e.g. with
Bti or an insect growth regulator such as methoprene
pellets in the water.
3. To kill the mosquitoes, we recommend two options that can be applied to the inner wall of the translucent chamber:
1 Heringer et al. (2016): Evaluation of alternative killing agents for Aedes aegypti in the Gravid Aedes Trap (GAT), Journal of
Medical Entomology
Option 2: Residual surface insecticides
A killing agent (e.g., residual surface spray insecticide) can
be applied to the inner wall of the translucent chamber [F4]
to kill mosquitoes through contact (for example Mortein
Outdoor Barrier Surface Spray, imiprothrin 0.3 g/kg and 0.6
g/kg deltamethrin Reckitt Bensckiser Pty. Ltd.)
Do not spray the black bucket or entry funnel. This will re-
pel mosquitoes!

6
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4. Put the catch bag over the open end of the transpa-
rent chamber. Tighten the top part of the catch bag by
pulling the cord of the bag tightly [F5 - F7]. You should
attach the catch bag with the seam facing outwards in
order to avoid mosquitoes getting stuck in the seam.
F5
F6
F7
F7
After strong or frequent rain the catch bag might hang into the water due to its own weight making the identication of the
captured mosquitoes even more dicult. If you cannot avoid placing the trap where it is subject to rain, we recommend
xing the catch bag in place with the mounting ring that is delivered with the trap:
F8 F9 F10
Start by laying the ring over the net on one side [F8]. Then press the ring equally on all sides down over the chamber [F9]
until the ring ts snuggly over the edge of the transparent chamber [F10].

7EN
F11
F12
F13
5. Insert the funnel in the chamber [F11].
6. Rotate the funnel clockwise to secure it [F12].
7. Mount the trap by placing the funnel and transparent
chamber on the black, water lled trap bucket [F13].

8
EN
Maintenance
Change the water (hay infusion or water with other attrac-
tants) once a month.
Remove any debris that falls into the trap or deters the mos-
quitoes from entering the trap.
If using oil or surface spray, retreat the translucent chamber
monthly.
Biogents AG
Weißenburgstr. 22
93055 Regensburg
Germany
www.biogents.com
Email: [email protected]om
Contact
Weight: 0.6 kg
Dimension: 27 x 38.5 cm
Removal of the Mosquitoes
Identication is complicated if the insects stay in the trap for
more than a week and dry out or if they get wet.
Removal of the mosquitoes:
Remove the funnel.
For direct sorting / identication in the eld: Loosen the
catch bag and remove it from the transparent chamber. Mos-
quitoes can then be removed with forceps or poured into a
white tray for sorting.
For returning the catch to the lab (e.g. for identication/
classication): Loosen the catch bag and remove it from the
transparent chamber. Then close the net by pulling the cord
of the catch bag tightly shut [F14]. It is best to put the catch
bag in a freezer for at least an hour to kill the mosquitoes.
Put a new catch bag over the open end of the transparent
chamber as described in F5 - F10.
Positioning
If you don‘t nd a naturally sheltered position for the trap, it would be best to position the trap under a roofed area, or a self
made shelter to keep it out of direct rainfall.
Even though rainfall does not damage the trap, there are some negative eects of rain:
• The mosquitoes can be damaged and dicult to identify.
• Mosquitoes avoid rain and prefer sheltered positions. In sheltered positions the catch rate can therefore be higher.
The black colour of the BG-GAT attracts mosquitoes from some distance. So, the trap needs to be placed where it is readily
visible, but protected from rain. Please do not hide the BG-GAT in the bushes!
Technical Data
Further information about the BG-GAT:
http://www.biogents.com/cms/website.php?id=/en/traps/biogents-trap-systems/bg_gat.htm
F14