Biohit eLine 730020 User manual

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SM
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Instra

e LINE®
Instruction Manual
Bedienungsanleitung
Mode d’ emploi
Manual Usario
Instruzioni d’impiego
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Instruction Manual ........................................1
Bedienungsanleitung ..................................35
Mode d’ emploi ..........................................71
Manual Usario ...........................................105
Instruzioni d’impiego ................................139
e LINE®
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CONTENTS
1. INTENDED USE ......................................................................................2
2. BIOHIT eLINE ELECTRONIC PIPETTES..............................................2
2.1. Biohit eLINE single-, 8- and 12-channel pipettes.......................2
2.2. Biohit tips......................................................................................3
3. UNPACKING............................................................................................3
4. PIPETTE DESCRIPTION........................................................................4
4.1. Display and controls ....................................................................4
4.2. Reset Button.................................................................................6
4.3. Start Button ..................................................................................6
4.4. Tip sealing and Electronic tip ejection ........................................6
4.5. Biohit Safe-Cone Filters...............................................................7
5. CHARGING OF THE PIPETTE...............................................................7
5.1. Charging with the Biohit eLINE Charging Stand and Carousel 8
5.2. Charging through direct charging system...................................9
5.3. Electrical specifications ...............................................................9
6. PROGRAMMING PRINCIPLE..............................................................10
6.1. Programming map...................................................................... 11
6.2. Speed adjustment......................................................................12
7. PIPET MODES....................................................................................... 12
7.1. Pipetting mode (P) and pipetting with mixing (P+MIXING).......12
7.2. Reverse pipetting (rP).................................................................13
7.3. Pipetting with cycle counter (P COUNT) ...................................14
7.4. Manual pipetting (P MANUAL)...................................................15
8. MULTI MODES......................................................................................16
8.1. Multi-dispensing (d).................................................................... 17
8.2. Diluting (dd) and diluting with mixing (dd+MIXING) ..................18
8.3. Sequenced dispensing (Sd).......................................................19
8.4. Multi-aspirating (SA)...................................................................20
8.5. Automatic dispensing (Ad).........................................................21
9. PROG MODE .........................................................................................22
9.1. Store operating mode to storage locations: .............................22
9.2. Recall stored mode from the storage location..........................23
10. PIPETTING RECOMMENDATIONS ....................................................23
10.1. Dispensing with blow-out ..........................................................23
10.2. Dispensing without blow-out.....................................................24
10.3. Other recommendations............................................................24
11. MAINTENANCE ....................................................................................24
11.1. Cleaning the outer surface of the pipette..................................24
11.2. Cleaning, decontaminating and autoclaving
the lower part of pipettes...........................................................25
11.2.1. Multichannel pipettes.................................................................26
11.3. Battery replacement...................................................................27
11.4. Storage .......................................................................................27
12. PERFORMANCE TESTING..................................................................27
12.1 Recalibration .............................................................................29
13. TROUBLE SHOOTING .........................................................................30
14. WARRANTY INFORMATION ...............................................................32
15. PERFORMANCE SPECIFICATIONS ...................................................32
16. RECYCLING INSTRUCTION (WEEE) .................................................34
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1. INTENDED USE
This liquid handling device is designed and manufactured to be used with
the IVD-product as an IVD-accessory and as a stand alone laboratory
equipment.
2. BIOHIT eLINE ELECTRONIC PIPETTES
The Biohit eLINE electronic pipettes offer you the latest technology and
innovations in the field of the hand held liquid handling devices. The attractive
and ergonomic design of the eLINE pipettes together with electronic tip
ejection operation take the effort out of pipetting and reduce the risk of
repetitive strain injuries (RSI) that are common in mechanical pipetting.
The microprocessor controlled system and the superior construction of
the Biohit eLINE pipettes enables maximum liquid handling performance
with high levels of accuracy and precision. The microprocessor controlled
piston movements reduces the possibility of human error and thus also
contamination of the pipette interior. The tip cone design of the Biohit eLINE
pipettes offer the possibility to use replaceable Safe-Cone Filters to help
prevent contamination and damage of the pipette.
The eLINE electronic pipettes offers a comprehensive range of liquid handling
protocols, typically required by laboratories currently. The simple keyboard and
easy-to-read display together with user-friendly operation and programming
allows these tasks to be performed quickly and effortlessly. The Biohit eLINE
pipettes operate on the air displacement principle and use disposable tips.
2.1. Biohit eLINE single-, 8- and 12-channel pipettes
Cat. No Colour Code Chan- nels Volume Range Incre- ment Tip
Safe Cone Filters
50 pcs/bag
Standard Plus
73002X
73004X
73006X
73008X
73010X
Grey
Yellow
Orange
Blue
Green
1-ch
1-ch
1-ch
1-ch
1-ch
0.2–10 μl
5–120 μl
10–300 μl
50–1000 μl
100–5000μl
0.05 μl
0.50 μl
1.00 μl
5.00 μl
10.00 μl
10 μl
300 μl, 350 μl
350 μl
1000 μl
5000 μl
N/A
721008
721007
721006
721006
N/A
721018
721017
721016
721016
73032X
73034X
73036X
73039X
Grey
Yellow
Orange
Violet
8-ch
8-ch
8-ch
8-ch
0.2–10 μl
5–120 μl
10–300 μl
50–1200μl
0.05 μl
0.50 μl
1.00 μl
5.00 μl
10 μl
300 μl, 350 μl
350 μl
1200 μl
N/A
721008
721007
721006
N/A
721018
721017
721016
73042X
73044X
73046X
73049X
Grey
Yellow
Orange
Violet
12-ch
12-ch
12-ch
12-ch
0.2–10 μl
5–120 μl
10–300 μl
50–1200μl
0.05 μl
0.50 μl
1.00 μl
5.00 μl
10 μl
300 μl, 350 μl
350 μl
1200 μl
N/A
721008
721007
721006
N/A
721018
721017
721016
X: 0 = Without AC-adaptor; 1 = With universal AC-adaptor (Euro, USA/Jpn, UK and
China plugs)
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Fig. 1.
2.2.Biohit tips
The full range of Biohit pipette tips
are recommended for use with Biohit
eLINE pipettes. Biohit standard tips
are made of virgin polypropylene.
Biohit also offers a full range of filter
tips. Biohit standard tips are available
as bulk packages, space-saving refill
systems and autoclavable (121°C, 1 bar
overpressure) trays. Pre-sterilized tips
in trays are also available (Fig. 1.)
3. UNPACKING
The Biohit eLINE electronic pipettes may be delivered with or without an AC-
adaptor, depending from the type of order. However, all Biohit eLINE pipette
packages contains:
• Pipette
• Tip
• Safe-Cone Filters and Filter Forceps (>10 μl)
• Grease (in single-channel units only)
• Instructions for use
• Performance certification in accordance with ISO 8655-6
Please check that all items are included and that no damage has occurred
during shipment.
Note: The battery is protected against discharging during storage with
a plastic protector. BEFORE USE PLEASE REMOVE THE PLASTIC
PROTECTOR UNDER THE BATTERY COVER.
Note: The pipette is supplied with discharged battery. It is recommended to
recharge the pipette for at least 12 hours before initial use.
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Operating Mode Symbol
Mode Range
Aliquots/Counter Volume
Speed in/out Battery Symbol
Aspirating/Dispensing direction
Display:
Display
Keyboard
Tip Ejection Buttons
Start Button
Handle
Handle Locking Collar
MCP Connecting
Collar
Tip Ejector Collar
(e10–e1000)
Tip Cone Housing
Tip Ejector Bar
Tip Cone(s)
(excl. 5000)
Safe-Cone Filter
Battery Cover
Charging
Contacts
Reset Button
Finger Hook
Tip Cone
(e5000)
Tip Ejector
Assembly
(e5000)
4. PIPETTE DESCRIPTION
4.1. Display and controls
The control and programming of the Biohit eLINE pipette is performed using
the display, keyboard and operating buttons.
Note: When the pipette is not connected to the charging unit the pipette/
display will automatically switch off after 10 minutes of non-use. Pipette can
be switched on by pressing any key.
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Keyboard:
Select Arrow up/down Enter
- Press to select - Press to select - Press to confirm
the mode range or the operating the selections or
to activate/confirm mode or to make setting changes
setting changes setting changes
(volume, aliquots,
speed, etc.)
Operating buttons:
Start button Tip ejection buttons Reset button
- Triggers all piston - Press to remove tip - Press to reset the
movements pipette
Mode Ranges and Operating Mode Symbols:
PROG
PROG 1-6
= 6 storage
locations
MULTI
d= multi-dispensing
dd = diluting
dd+MIXING = diluting
with mixing
Sd = sequencial
dispensing
SA = multi-aspirating
Ad = automatic
multi-dispensing
PIPET
P= pipetting
rP = reverse
pipetting
P+MIXING = pipetting
with mixing
P COUNT = pipetting with
cycle counter
P MANUAL* = manual
pipetting
*Not available in multichannels
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Fig. 2.
4.2. Reset Button
To reset the pipette, press the reset button on the battery cover. The display
shows RESET and PRESS TIP in a blinking form and pressing the tip ejector
button will reset the pipette to the first programming level in PIPET mode.
During the reset function the pipette automatically determines the home
position for the piston and tip ejector by running the piston up and down.
During the reset function the display shows AUTO TEST and the completed
reset function is confirmed by showing the text DONE on the display.
Note: The pipette must never be reset with the tip attached! The attached
tip can induce an error in home position determination. However, if resetting
is accidentally done with the tip attached reset the pipette again without the
tip attached.
4.3. Start Button
The start button triggers the aspiration and dispensing operations according
to selected operating mode. Only a soft touch is required to operate the
button. If the start button is held down during dispensing, the piston will stop
in the lowest position until the button is released. In the operating modes with
automatic blow-out (P, dd) the piston will automatically return to the home
position after the start button is released.
If the start button is held down in the reverse pipetting mode or during the
dispensing of the last aliquot in the multi-dispensing modes the piston
direction symbol will change within one second and the pipette will be ready
to aspirate the next sample when the start button is released.
4.4. Tip sealing and
Electronic tip ejection
The Biohit pipette tips are recommended for use
with Biohit eLINE pipettes. The eLINE multichannel
pipettes are provided with a spring loaded
OPTILOAD tip cones for optimal sealing.
For easy and effortless tip ejection all Biohit eLINE
electronic pipettes are equipped with the new
electronic tip ejection feature (Fig. 2). There are
two tip ejection buttons conveniently placed on the
either side of the start button to ensure easy reach
for both right and left handed users.
Tip ejection: When the pipetting cycle is
completed, either of the buttons can be depressed
to eject the tip.
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Note: The tip ejection function is frozen during the pipetting cycle and can
only be activated after the tip is emptied. If needed the pipetting cycle can be
interrupted by pressing the enter button. The display will show E (E=empty)
and the tip is then emptied by pressing the start button.
4.5. Biohit Safe-Cone Filters
The tip cone design of the Biohit eLINE
pipettes (>10 μl) allows the use of Biohit
Safe-Cone Filters in the tip cones. These
removable filters prevent liquids and
liquid vapours from entering the pipette
and thus protects the pipette from cross
contamination and damage.
The Safe-Cone Filters are available
as Standard or Plus versions. It is
recommended to use the Standard Filter for
general applications and the Plus Filter for
more demanding applications. The filters
need to be changed regularly. The interval
for filter changing is application dependant
but the recommendation is to change the
filter daily (after 50 – 250 pipetting cycles)
and always in case of over-aspiration. The
Biohit Filter Forceps should be used to avoid
touching the soiled filters by hand (Fig. 3).
(See Chapter 2.1. for ordering information.)
5. CHARGING OF THE PIPETTOR
The battery is protected against discharging
during storage with a plastic protector.
BEFORE USE PLEASE REMOVE THE PLASTIC
PROTECTOR UNDER THE BATTERY COVER.
However, should the battery become discharged
during storage and recharging is required, a battery
symbol will be observed in the bottom right corner
of the display (Fig. 4).
The Biohit eLINE pipettes should be charged using the original Biohit eLINE
Charging Stand or Charging Carousel. However, the Biohit eLINE also
features direct charging by using the Biohit eLINE AC-adaptor.
Note: If the battery is fully discharged, the display will be empty and there will
be no response from the operating buttons. After a few minutes on charge,
the battery symbol will be displayed and the + and – symbols will continue
to blink until the battery is fully charged.
Fig. 3.
Fig. 4.
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5.1. Charging with the Biohit eLINE Charging
Stand and Carousel
The Biohit eLINE Charging Stands and
Carousels are bench top charging units.
The Charging Stand accommodates one
Biohit eLINE pipette and the Charging
Carousel four Biohit eLINE pipettes. The
eLINE Charging Stands and Carousels
operate through the metal contacts placed
in the sides of the pipette and in the head
of the Charging units (Fig. 5.).
Before operating the AC-adaptor must be
connected to the socket at the rear of the
Charging Stand (Fig. 5) or at the side of
the Charging Carousel as well as to the
electrical outlet. Before connecting the
AC-adaptor to the electrical outlet, check
that its voltage settings corresponds to the
voltage settings of your electrical outlet.
Cat. No. Product
730981 Biohit eLINE Charging Stand
730991 Biohit eLINE Charging Carousel
With universal AC-adaptor (Euro, USA/Jpn, UK and China plugs)
1. Check that the battery protector is removed and the battery cover is
properly closed.
2. Place the pipette into the Charging unit. Make sure that the metal
contacts at the rear of the pipette are properly fitted to the tracks of the
metal contacts in the top of the Charging unit.
3. When charging, the battery symbol will be displayed with the + and –
symbols flashing. The charging time for completely discharged batteries
is 4 hours.
4. When the battery is full the battery symbol disappears from the display.
If display shows RESET and PRESS TIP, press the tip ejection button to
reset the pipette, the pipette will then be ready for use.
Note: Never reset the pipette with the tip attached as the attached tip can
induce an error in the home position determination of the piston and tip
ejector collar!
Note: When the pipette is not in use it is recommended to keep the pipette
in the Charging unit. This ensures that the battery is kept charged and the
pipette is ready for use.
Fig. 5.
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5.2. Charging through direct charging system
The AC-adaptor supplied with the Biohit eLINE pipette
or single Charging Stand can be used for direct charging
of the Biohit eLINE electronic pipette. The socket for the
AC-adaptor is placed at the back of the pipette (Fig. 6).
1. Before connecting the AC-adaptor to the electrical
outlet, check that its voltage settings corresponds
to the voltage settings of your electrical outlet.
2. Check that the battery protector is removed and the
battery cover is properly closed.
3. Connect the AC-adaptor to the electrical outlet as
well as to the socket at the back of the pipette.
4. The charging time is 12 hours for a completely
discharged battery.
Note: It is possible to continue pipetting while the eLINE
pipette is charged through the AC-adaptor (Fig. 7). With
fully discharged battery a 30 minute initial charging is
required before continuing the pipetting.
5.3. Electrical specifications
Battery - Rechargeable NiMH battery pack with protection circuit
- 4.8 V, 500 mAH
- Charging time min 12 hours for empty batteries
AC-adaptor for Charging Stand or direct charging
- Input voltages and main plug according to local requirements
- Output voltage 7.5 VDC, 300 mA
AC-adaptor for Charging Carousel
- Input voltages and main plug according to local requirements
- Output voltage 9 VDC, 1200 mA
Fig. 7.
Fig. 6.
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6. PROGRAMMING PRINCIPLE
The Biohit eLINE pipettes features 10-11 different operating modes and six
different storage locations for frequently used modes with user-selected
settings. The operating modes are divided in to three Mode Ranges (PIPET,
MULTI, PROG) and each Mode Range includes the following operating
modes:
Mode Range: Operating Modes:
PIPET P, rP, P+MIXING, P COUNT, P MANUAL*
MULTI d, dd, dd+MIXING, Sd, SA, Ad
PROG 1, 2, 3, 4, 5, 6 (=storage locations)
Programming is performed by using the Select, Enter and arrow keys. The
mode range is selected with the Select key, the desired operating mode is
then selected with the arrow key and selection confirmed with Enter key.
The setting changes (volumes, aliquots, etc.) are made with arrow keys and
confirmed with either Select or Enter key. In the shortcut programming the
changes are confirmed with the Enter key. In the shortcut programming the
speed adjustment is not possible. The use of the Select key in confirming the
setting changes enables also the speed adjustment.
Note: When the programming or pipetting cycle is completed it is possible
to make the setting changes inside the operating mode simply by pressing
the arrow key and confirming with Enter or Select.
The Biohit eLINE pipette includes also six storage locations (Prog 1–6). The
operating mode with user-selected settings can be stored in to the storage
location under PROG mode for future recalls. After completing the setting
changes of the selected operating mode simply press Enter and Select keys
simultaneously, choose the storage location (PROG 1-6) with arrow key and
press Enter. The stored operation mode can now be recalled from the PROG
mode.
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PIPET MULTI PROG
P d PROG 1
rP dd PROG 2
P
+ MIXING dd + MIXING PROG 3
P MANUAL* Sd PROG 4
P COUNT SA PROG 5
Ad PROG 6
To change all settings To change settings When pre-stored
including speed: excluding speed: Prog 1–6 was chosen
Start pipetting
Store the
operating
mode with
Change selected
settings with settings to
Prog 1–6
+
Start pipetting +
* P MANUAL is not available in
multichannel models. Start pipetting Start pipetting
or
or
Repeat until
programming
is complete
or
6.1. Programming map
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6.2. Speed adjustment
There are 9 speeds available for both dispensing and aspiration. The speed
is indicated in the bottom left hand corner of the display. The default speed
is 5 for all operating modes.
Speed can be adjusted during the programming by completing the setting
changes with the select key. In every operating mode the speed adjustment is
the last programming step. After adjusting the “speed out” the programming
is completed with enter or select key and the pipette is then ready for
operation.
Note: The P MANUAL mode includes 5 different dispensing and aspirating
speeds.
7. PIPET MODES
The mode range PIPET includes 5 different operating modes.
Symbol Description
P Pipetting with automatic blow-out
rP Reverse pipetting without blow-out
P + MIXING Pipetting with blow-out followed with mixing function
P COUNT Pipetting with blow-out and displayed cycle counter
P MANUAL Manual pipetting with on-line volume display
7.1. Pipetting mode (P) and pipetting with mixing (P+MIXING)
In pipetting mode (P) the eLINE performs pipetting with blow-out. In pipetting
with mixing mode (P+MIXING) pipetting is followed by a mixing function.
1. Press to display PIPET
2. Scroll with until the symbol P or P+MIXING is displayed.
3. Confirm by pressing
4. Select the desired pipetting volume by using the
Note: When holding down the the volume display starts to scroll faster.
5. Press to confirm selection and to display the speed in or press
and continue from Step 10.
6. Select the speed in with (1= Min and 9 = Max)
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▲
▼
7. Pre s s or to confirm selection and to display the speed out
8. Select the speed out with (1= Min and 9 = Max)
9. Press or to confirm selections
10. Position the tip to aspirate and press the START button
11. Position the tip to dispense. Press the START button to dispense
the sample. The tip is emptied with blow-out and the piston returns
automatically back to the home position.
Step 10. Step 11.
Note: When the START button is held down during dispensing the piston will
stop in the lowest position. When the start button is released the piston will
automatically return to the home position.
To mix:
1. Position the tip in to the solution, then press and hold the START button
.
Mixing is performed automatically as long as the START button is
held down.
2. Position the tip to dispense. Press START button twice
Note: The mixing is performed with about 70% of the total volume.
7.2. Reverse pipetting (rP)
A selected volume plus and excess is aspirated into the tip. After dispensing
the selected volume the excess volume remains in the tip and is discarded.
1. Press to display PIPET
2. Scroll with until the symbol rP is displayed.
3. Confirm by pressing
4. Select the desired pipetting volume by using
Note: When holding down the volume display starts to scroll faster.
5. Press to confirm selection and to display the speed in
or press and continue from Step 10.
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6. Select the speed in with (1= Min and 9 = Max)
7. Pre s s or to confirm selection and to display the speed out
8. Select the speed out with (1= Min and 9 = Max)
9. Press or to confirm selections
10. Position the tip to aspirate and press the START button
11. Position the tip to dispense. Press the START button
12. Position the tip to discard excess liquid and press the START button
twice
Step 10 Step 11 Step 12
Note: It is also possible to continue to aspirate and dispense the same volume
without the empty function. To continue, keep the START button depressed
during dispensing and within one second the direction arrow will change.
Continuing to hold the START button down, place the tip into the liquid again
and then release the START button to aspirate the next sample.
7.3. Pipetting with cycle counter (P COUNT)
The eLINE performs pipetting with blow-out and the cycle counter function.
The cycle counter counts up to 99 pipettings and then returns back to 0. It
is possible to choose the cycle number to begin with. It is also possible to
change the cycle number or reset the counter during the pipetting by using
the arrow keys.
1. Press to display PIPET
2. Scroll with until the symbol P and COUNT is displayed.
3. Confirm by pressing
4. Select the desired pipetting volume by using the
Note: When holding down the volume display starts to scroll faster.
5. Press or to confirm selection.
6. Cycle counter will show 1. Use to select the sample number you want
to begin with (0-99).
7. Pre s s to confirm the selection and to display the speed in
or press and continue from Step 12.
▲
▼▼
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▲
▼
8. Select the speed in with (1= Min and 9 = Max)
9. Press or to confirm selection and to display the speed out
10. Select the speed out with (1= Min and 9 = Max)
11. Press or to confirm selections
12. Position the tip to aspirate and press the START button
13. Position the tip to dispense and press the START button . The tip is
emptied with blow-out and the piston returns automatically back to the
home position. The pipette is ready for next pipetting.
Step 12 Step 13
Note: To change the cycle number or to reset the counter during the pipetting,
press the to display the desired cycle number and confirm with .
7.4. Manual pipetting (P MANUAL)
(Not available in multichannel models)
In the manual pipetting mode the aspiration and dispensing are controlled
manually. The aspiration or dispensing continues as long as the START
button is held down (within the selected volume range). The aspiration/
dispensing direction can be changed during pipetting by using the arrow key.
In the manual pipetting mode the display always shows the actual amount
of sample in the tip.
1. Press to display PIPET
2. Scroll with until the symbol P MANUAL is displayed.
3. Confirm by pressing
4. Select the desired pipetting volume by using
Note: When holding down the volume display starts to scroll faster.
5. Press to confirm selection and to display the speed in
or press or continue from Step 10.
6. Select the speed in with (1= Min and 5 = Max)
7. Press or to confirm selection and to display the speed out
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▼▼
▲
8. Select the speed out with (1= Min and 5 = Max)
9. Press or to confirm selections
10. Position the tip to aspirate and press the START button
Step 10
Note: The aspiration can be interrupted at any time simply by releasing the
START button and continued by pressing the START button again. The
aspiration/dispensing direction can be changed at any time by pressing the
arrow key.
11. Press the to change the aspiraton/dispensing direction.
12. Position the tip to dispense. Press the START button
13. When the tip is empty (volume reaches 0 μl), within one second the
display will show E. Position the tip to empty the tip with blow-out and
press the START button twice .
Step 12 Step 13
8. MULTI MODES
The mode range MULTI includes 6 different operating modes.
Symbol Description
d Multi-Dispensing
dd Diluting
dd + MIXING Diluting with mixing function
Sd Sequenced dispensing
SA Multi-aspirating
Ad Automatic multi-dispensing with selected interval
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8.1. Multi-dispensing (d)
The pipette performs repetitive dispensing of a selected volume. The sum
of the dispensing aliquots and an automatically selected excess volume is
aspirated into the tip. The excess volume is needed to ensure equal operating
conditions for each dispensing step.
1. Press to display MULTI
2. Scroll with until the symbol d is displayed.
3. Confirm by pressing
4. Select the desired dispensing volume by using
Note: When holding down the the volume display starts to scroll faster.
5. Press or to confirm the selection and to display the aliquots
6. Select the desired number of aliquots with
7. Pre s s to confirm selection and to display the speed in or press
and continue from Step 12.
8. Select the speed in with (1= Min and 9 = Max)
9. Press or to confirm selection and to display the speed out
10. Select the speed out with (1= Min and 9 = Max)
11. Press or to confirm selections
12. Position the tip to aspirate and press the START button . The
display shows PRE OUT and arrow down to indicate the reset function.
13 . Position the tip to discard priming excess and press the start button .
14. To dispense, position the tip, press START button and repeat until
the cycle is complete.
15. Finally position the tip to discard any remaining excess and press the
START button twice .
Step 12 Step 13 Step 14 Step 15
Note: It is also possible to continue to aspirate and dispense the same volume
without the empty function. To continue, keep the START button pressed
during the last dispensing and within one second the direction arrow will
change. Keeping the START button depressed, place the tip into the liquid
again and then release the START button to aspirate the next sample.
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