Biohit Proline 710520 User manual

Proline®
Käyttöohje
Bruksanvisning
Instruction Manual

Käyttöohje.....................................................1
Bruksanvisning ...........................................23
Instruction Manual ......................................45
Specifications .............................................67
Proline®

45
CONTENTS
1. INTENDED USE ..................................................................................46
2. YOUR NEW BIOHIT PROLINE ELECTRONIC PIPETTE.................46
2.1. Biohit Proline single-channel electronic pipettes..................46
2.2. Biohit Proline multichannel electronic pipettes .....................47
2.3. Biohit tips ................................................................................ 47
3. UNPACKING & PREPARING THE PIPETTE FOR USE ...................48
3.1. Biohit Proline Charging Stands and Carousels .....................48
3.2. Charging of the Pipette...........................................................49
3.3. Electrical Specifications .........................................................49
4. PIPETTE MATERIALS ........................................................................50
5. PIPETTE DESCRIPTION....................................................................51
5.1. Controls ................................................................................... 51
5.2. START Button.......................................................................... 51
5.3. Direction Symbols................................................................... 51
5.4. Display ..................................................................................... 51
5.5. Sealing and Ejecting tips ........................................................52
5.6. Optional Filters ........................................................................52
6. PROGRAMMING THE PIPETTOR ....................................................53
6.1. Mode Selection and Mode Recall ..........................................53
6.2. Setting Speeds .......................................................................53
6.3. Pipetting Mode (P) ..................................................................54
6.4. Reverse Pipetting Mode (rP)...................................................54
6.5. Multi-Dispensing Mode (d) .....................................................55
6.6. Diluting Mode (dd)...................................................................56
6.7. Mixing Mode with Pipetting or Diluting (*)..............................56
6.8. Sequenced Dispensing Mode (sd) .........................................58
6.9. Multi-Aspirating Mode (SA) ....................................................58
7. PIPETTING RECOMMENDATIONS ..................................................59
7.1. D is pen sin g wi th Bl ow- o ut.......................................................59
7.2. Dispensing without Blow-out .................................................60
7.3. Other Recommendations .......................................................60
8. STORAGE ............................................................................................60
9. CALIBRATION.....................................................................................61
9.1. Testing the Performance.........................................................62
10. MAINTENANCE ..................................................................................62
10.1. Cleaning the tip cone ..............................................................63
10.2. Battery Replacement..............................................................63
11. TROUBLE-SHOOTING.......................................................................64
12. WARRANTY INFORMATION .............................................................66
13. RECYCLING INSTRUCTION (WEEE) ...............................................66

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1. INTENDED USE
This liquid handling device is designed and manufactured to be used with
the IVD-product as an IVD-accessory and as a stand alone laboratory
equipment.
2. YOUR NEW BIOHIT PROLINE ELECTRONIC PIPETTE
Your new Biohit Proline Electronic pipette has been designed for maximum
performance and accuracy with ergonomic and user-friendly operation.
The microprocessor controlled system reduces the possibility for human
error and instrument contamination by controlling all piston movements.
Also selected models offer replaceable tip cone filters to help prevent
contamination and damage.
Its light-weight and ergonomic controls take the effort out of pipetting to
help reduce the risk of repetitive strain injuries (RSI) that are frequent in
manual pipetting. All Biohit Proline Electronic pipettes operate on the air
displacement principle and use disposable tips.
2.1. Biohit Proline single-channel electronic pipettes
Cat. No. Volume Range Increment Tip
710520 0.2 -10 μl0.1μ10 μl
710010 5 - 100 μl1μl300μl, 350 μl
710030 10 - 250 μl5μl300μl, 350 μl
710100 10 - 500 μl5μl 1000 μl
710020 50 - 1000 μl10μl 1000 μl
710040 50 - 1200 μl5μl1200μl
710500 100 - 5000μl50μl 5000 μl

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2.2.Biohit Proline multichannel electronic pipettes
Cat. No. Volume Range Increment Tip
710410 4-kan 5 -100 μl1μl300μl, 350 μl
710420 4-kan 25 - 250 μl5μl350μl
710200 8-kan 0.2 - 10 μl0.1μl10μl
710210 8-kan 5 - 100 μl1μl300μl, 350 μl
710220 8-kan 25 - 250 μl5μl350μl
710800 8-kan 50 - 1200 μ10 μl1200μl
710300 12-kan 0.2 - 10 μl0.1μl10μl
710310 12-kan 5 - 100 μl1μl300μl, 350 μl
710320 12-kan 25 - 250 μl5μl350μl
710810 12-kan 50 - 1200 μl10μl1200μl
2.3. Biohit tips
The full range of Biohit tips are recommended
for use with Biohit pipettes. These detachable,
disposable tips are made of natural colour
polypropylene. Biohit also offers a full range
of filter tips. Biohit standard tips are available
as bulk packages, space saving refill systems
and autoclavable (121°C, 20 min, 1 atm) trays.
Presterilized tips in trays are also available.
(Fig. 1).
Note: Never pipette liquid without attaching
a tip to the pipette.
Fig. 1

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3. UNPACKING & PREPARING THE PIPETTE FOR USE
The Biohit Proline Electronic pipette package contains the following items:
• Pipette
• Tips
• Grease
• Filters and filter forceps (selected models, see page 8)
• Instructions for use
• Quick reference card
• Performance certificate in accordance with ISO 8655-6
Make sure that all items are included and that no damage has occured
during shipment.
Note: The pipette can only be charged with the Biohit Proline charging
stand or carousel (Fig. 2 and 3).
3.1. Biohit Proline Charging Stands and Carousels
Cat. No. Product
51000X Biohit Proline Charging Stand
51160X Biohit Proline Charging Carousel
X: 1=Euro, 2=U.S., 3=U.K., 4=Jpn
Fig. 2 Fig. 3

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Fig. 5
Fig. 4
3.2. Charging of the Pipette
The charging stand or carousel operates via a contactless system which is
free from maintenance and sensitive metal contacts. The pipette features
an ON/OFF switch located at the top of the grip of the pipette (Fig. 4). This
switch protects the battery from discharging during long storage periods.
1. Switch the pipette ON (the far left position) (Fig. 4).
2. Place the pipette into the charging stand. The top of the pipette will fit
snugly into the head of the charging stand or carousel. The magnetic
mount will lock the pipette into the proper charging position (Fig. 5).
3. If the pipette is new or the battery is low keep the pipette in the stand
for 12 hours to fully charge the pipette before continuing use.
4. Display shows . Press the START button twice and the pipette
is ready for the default pipetting at maximum volume or for program
changes.
Note: The pipette will charge in both the ON and OFF positions. Leave
the pipette ON in the charging stand to retain user-selected settings as
switching OFF will reset the unit to default settings.
Note: If the pipette is left in the ON position uncharged for several days
the display will be empty and there will be no response from the keyboard,
or START button, as the battery voltage will be below the operating level.
3.3. Electrical Specifications
Battery
• Rechargeable NiMH battery
• Charging time max 12 hours for empty battery
AC-Adaptor
• Input voltage and main plug according to local requirements
• Output voltage 9 VDC

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Tip cone
10 μl (PVC)
100-1000 μl (SS)
1200 μl (PPS)
5000 μl (PE)
Tip ejector
collar (PP)
Tip ejector
level (POM)
Display
(glass)
Finger
support
(back)
(ASA/PC)
Tip ejector
level (POM)
Display
(glass)
Finger
support
(back)
(ABS)
Keyboard
(silicon)
Start
button
(POM)
Keyboard
(silicon)
Start
button
(POM)
Handle
(ASA/PC)
Handle
(ASA/PC)
Tip cone
housing
(ASA/
PC)
Tip ejector
bar (PETP)
Tip cone, soft-sealing
10-250 μl (sealing: TPU)
10-250 μl (PVDF)
1200 μl (PPS)
Hard tip cone
(PC)
Accu cover
(ASA/PC)
Accu cover
(ASA/PC)
Level support
(PETP)
4. PIPETTE MATERIALS

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Battery Compartment
Power Switch
Changes speeds
Moves to Next Mode
Increases volume or speed
Decreases volume or speed
Confirms setting changes
Switches Mixing on - off
START BUTTON Triggers all mechanical operations
TIP EJECTOR LEVER Squeezed to remove pipette
tip(s)
5. PIPETTE DESCRIPTION
The control and programming of the Proline electronic pipette are done
using the keyboard and display shown in detail below.
5.1. Controls
5.2. START Button
The START button triggers the aspiration and dispensing operations
according to selected operating mode. Only a quick click is required to
operate the button. If the START button is kept down, the piston will stop in
the lowest position until the button is released. This feature is applicable to
all modes of operation, except for multiple dispensing (d).
5.3. Direction Symbols
These symbols indicate the direction in which the piston moves upon
pressing the START button. The small RIGHT ARROW in the display means
that the next function is to aspirate the liquid. The small LEFT ARROW
in turn indicates the dispensing function in accordance to the selected
operating mode.
5.4. Display
The left display is the status indicator. It informs the user about parameters
to be programmed, functions to be performed and the number of
dispensings available.
The right display is used for programming and displaying the various
volumes needed in different operating modes.

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N/A =
Not
available
5.5. Sealing and Ejecting tips
Biohit tips are recommended for use with Proline
pipettes. Before fitting a tip make sure that the
pipette tip cone is clean. Press the tip onto the
cone of the pipette firmly to ensure an airtight seal.
The seal is tight when a visible sealing ring forms
between the tip and the tip cone (Fig. 6).
Biohit Proline Electronic pipettes are designed for
simple and light attachment and ejection of the tips.
To eject the tip, simply place the pipette over the
discard container and squeeze the tip ejector lever
(Fig. 7).
5.6. Optional Filters
The new tip cones of selected Biohit Proline
Electronic pipettes allow the use of a removable
filter as an option. The filter prevents liquids and
liquid vapours from entering the pipette. The filter
does not affect the calibration of the pipette. Biohit
filter forceps should be used to avoid touching the
dirty filters by hand. (Fig. 8).
Cat. No Channels Volym Range Standard
Filter
Plus Filter
710520
710010
710030
710100
710020
710040
710500
1-kan
1-kan
1-kan
1-kan
1-kan
1-kan
1-kan
0.2 - 10 μl
5 - 100 μl
10 - 250 μl
10 - 500 μl
50 - 1000 μl
50 - 1200 μl
100-5000 μl
-
-
-
721006
721006
721006
721006
-
-
-
721016
721016
721016
721016
710410
710420
4-kan
4-kan
5 - 100 μl
25 - 250 μl
721014
721014
-
-
710200
710210
710220
710800
8-kan
8-kan
8-kan
8-kan
0.2 - 10 μl
5 -100 μl
25 - 250 μl
50 - 1200 μl
-
721014
721014
721006
-
N/A
N/A
721016
710300
710310
710320
710810
12-kan
12-kan
12-kan
12-kan
0.2 - 10 μl
5 - 100 μl
25 - 250 μl
50 - 1200 μl
-
721014
721014
721006
-
-
-
721016
Fig. 6
Fig. 7
Fig. 8

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6. PROGRAMMING THE PIPETTOR
Programming is done using the six-button keyboard and the LC-display.
There are four to six different operating modes, offering special functions
and varying speeds for your selection.
6.1. Mode Selection and Mode Recall
1. Press repeatedly to view the available modes of the pipette.
2. Press when the desired mode is displayed. The pipette is ready
for use in the mode selected.
Note: Mode can only be activated when the piston is in its home position
(arrow right sign is lit), not in the middle of aspiration or dispensing actions.
6.2. Setting Speeds (all models)
1. Press to display the current aspiration speed.
2. Press or until desired aspiration speed is displayed (”5”
Fast and ”1” Slow).
3. Press to confirm speed selection. Display shows the current
dispense speed.
4. Press or until desired dispense speed is displayed
(”5” being Fast and ”1” Slow).
5. Press to confirm speed selection.
Note: The speed may not be changed in the middle of the aspiration or
dispensing cycles.
Note: The default speed is 3 for all speed settings.

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6.3. Pipetting Mode (P) (all models)
The pipette performs ordinary pipetting with blow-out.
1. Press to display .
2. Press to confir the mode change.
3. Select the desired pipetting volume by using to increase, and
to decrease.
Note: when holding down or , the volume display starts to
scroll faster.
4. Press to confirm selection.
5. Position the tip to aspirate and press the
START button.
6. Position the tip to dispense. Press the START
button. The tip is emptied with the blow-out
and is ready for next pipetting.
6.4. Reverse Pipetting Mode (rP)
(all models, except 710040, 710500, 710800 och 710810)
A Selected volume plus an excess is aspirated into the tip. After delivery, the
excess volume remains in the tip and is discarded.
1. Press until is displayed.
2. Press to confirm mode change.
3. Use and to select the desired pipetting volume.
4. Press to confirm selection.
5. Position the tip to aspirate and press the START button.
6. Position the tip to dispense. Press the START button.
7. Position the tip to discard excess liquid and press START
twice.
Note: Upon delivering the programmed volume, it is also
possible to continue to aspirate and dispense the same
volume without the empty function. To continue, keep the
START volume without the empty function. To continue, keep
the START button pressed down and within one second the
direction of the arrow will change. Keeping the button down,
place the tip into the liquid again and the sample is aspirated
into the tip by releasing the START button.
Step 5. Step 6.
Step 5.
Step 6.
Step 7.

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6.5. Multi-Dispensing Mode (d)
(all models)
The pipette performs repetitive dispensings of a selected volume. During
this operation, the desires volume plus and automatically selected excess
volume is aspirated into the tip. The excess volume is needed to ensure
equal operating conditions for each dispensing.
1. Press to display .
2. Press to confirm the mode change.
3. Press or until the multi-dispense volume is displayed.
4. Press to confirm selection.
5. Press or until the desired number of aliquots is displayed.
6. Press to confirm selection.
7. Position the tip to aspirate and press the START button.
The * sign and ARROW LEFT are lit to indicate the reset function.
8. Position the tip to discard priming excess and press the START button.
9. To dispense, position the tip, press the START button and repeat unti
the cycle is complete.
10. Finally, position the tip to discard any remaining excess and press the
START button twice.
Note: Upon delivering the programmed volume, it is also possible to
continue to aspirate and dispense the same volume without the empty
function (excl. 710500). To continue, keep the START button pressed down
and within one second the direction of the arrow will change. Keeping the
button down, place the tip into the liquid again and the sample is aspirated
into the tip by releasing the START button.
Step 10.
Step 9.Step 7. Step 8.

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6.6. Diluting Mode (dd)
(all models, except 710800 and 710810)
Two different solutions separated by an air gap are aspirated and then
dispensed together with automatic blow-out. The purpose of the air gap is
to avoid contamination when aspirating the second volume but it will not
prevent the two liquids from mixing in the tip.
1. Press to display .
2. Confirm the mode change by pressing .
3. Select the desired diluent volume (volume 1) by using and .
4. Confirm by pressing .
5. Press and to select the sample volume (volume 2) on the
display.
6. Press to confirm selection.
7. Position the tip to aspirate volume 1 and then press the START button.
8. With the tip in the air press the START button again to aspirate an air
gap.
9. Position the tip to aspirate volume 2 and press the START button.
10. Finally, position the tip to dispense and press the START button.
6.7. Mixing Mode with Pipetting or Diluting (*)
(all models)
The piston is automatically moved up and down to mix the liquid in the
delivery vessel. The mixing time is controlled by the START button.
1. Use to select either or onto the display.
2. Press to switch on mixing. The display should read either or
. Please note that selecting will alternately switch mixing
on and off.
3. Confirm the mode change by pressing the button.
Step 7. Step 10.
Step 8. Step 9.

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For Pipetting:
1. Press to increase, and to decrease.
2. Press to confirm selection.
3. Position the tip to aspirate and press START button.
4. Position to dispense and press the START button.
For Diluting:
1. Select the desired diluent volume (volume 1) by using and .
2. Confirm selection by pressing .
3. Press and to select the sample volume (volume 2) .
4. Confirm selection by pressing .
5. Position the tip to aspirate volume 1 and then press the START button.
6. With the tip in the air, press the START button to aspirate an air gap.
7. Position the tip to aspirate volume 2 and press the START button.
8. Finally, position the tip to dispense and press the START button.
To Mix:
1. Position the tip in the solution, then press and hold the START button.
The mixing is done automatically as long as the START button is held
down.
2. Position the tip to dispense. Press the START button twice.
Note: The mixing is done with about 70 % of the total volume.
Step 1. Step 2.

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6.8. Sequenced Dispensing Mode (sd)
(models 710500, 710040 and 710800 only)
A series of different volumes can be delivered in any desired order.
1. Press to display .
2. Confirm the mode change by pressing .
3. Select the first desired dispense volume onto the display by using
and .
4. Press to confirm the dispense volume.
5. To select the following dispense volumes (up to 12) use and
always remembering to confirm each selection by pressing .
6. To confirm your final selection press .
7. Position the tip to aspirate and press the START button.
8. Position the tip to discard priming excess and press the START button
again.
9. Position the tip to dispense and press the START button. Repeat the
action until the cycle is complete.
10. Position the tip to discard any remaining excess and press the START
button twice.
6.9. Multi-Aspirating Mode (SA)
(models 710040, 710800 and 710810)
The pipette performs consecutive aspiration operations of the programmed
volume. This mode allows e.g. emptying a microwell plate and other
special applications.
1. Set the display to by pressing .
2. Confirm the mode change by pressing .
3. Select the desired multi-aspiration volume by using or .
Step 7. Step 10.
Step 8. Step 9.

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4. Confirm selection by pressing .
5. Press and to select the number of aspirations desired.
6. Confirm your selection by pressing .
7. Position the tip to aspirate and press the START button, repeating the
action until the cycle is complete. The total volume is displayed.
8. Position the tip to dispense and press the START button.
Note: The aspiration can be interrupted by pressing . An ARROW
and E appear in the display. Pressing the START button will empty the tip.
7. PIPETTING RECOMMENDATIONS
By using the different operating modes and various special functions,
several different liquid handling procedures are made possible. Two of
the modes (P and dd) feature an automatic blow-out and others leave an
excess liquid in the tip. The user should follow the recommendations below
to ensure optimal performance.
7.1. Dispensing with Blow-out
The pipetting and diluting mode have an automatic blow-out function,
followed by an immediate return of the piston to the ”home” position. To
avoid accidental aspiration of the liquid back into the tip, it is recommended
that the dispensing is always done above the liquid surface.
By holding the START button down during dispensing the piston will stop
in the lowest position. This allows the tip to be placed against the bottom
or the wall of the container. Once the liquid is dispensed, the tip can be
removed from the container and the START button released.
Step 7. Step 8.

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7.2. Dispensing without Blow-out
The pipette will not perform the blow-out function when using the reverse
pipetting and dispensing modes. Therefore, it is recommended that
dispensing is always performed with the tip set against the wall or the
bottom of the container. The use of the reverse pipetting mode is especially
useful when pipetting small volumes or solutions that have a tendency to
foam or have a high viscosity.
7.3. Other Recommendations
• Hold the pipette vertically when aspirating the liquid and place the tip
only a few millimetres into the liquid.
• Pre-rinse the tip before aspirating the liquid by filling and emptying the
tip for three to five times. This is important especially when dispensing
liquids with a viscosity and density greater than water or liquids with
high vapor pressure (e.g. ethanol).
• When pipetting liquids with temperatures different to the ambient
temperature do not pre-rinse the tip and change the tip after each
pipetting.
• Check the pipette, tip and liquid are at room temperature.
• Avoid contaminating the tip cone, do not rest the pipette on its side.
• Always place the pipette in the charging stand or carousel when it is
not in use.
• Never strike the tip cone against the tip tray when mounting tips as this
can damage several internal components.
• Do not drop the pipette, the charging stand, carousel or AC-adaptor as
this may cause excessive shock.
• Avoid exposing the unit to extreme temperature changes, humidity and
dust (operating temperature from 15°C to 40°C).
• Avoid rough handling, moderate pressure is all that is required when
using the keyboard.
• Avoid leaving the pipette on its side with liquid in the tip which might
seep back into the mechanism.
• Always pipette against the inside wall of the receiving vessel. Remove
the tip by drawing it up against the inside wall.
• Change the filter on the tip cone regularly (after 50-250 pipettings).
8. STORAGE
When not in use it is recommended that the pipettor is stored in the ON
position in the Biohit Proline charging stand or carousel.