Biohit mLINE m10 User manual

mLINE®
Instruction Manual
Bedienungsanleitung
Mode d’ emploi
Manual Usuario
Instruzioni d’impiego

Instruction Manual . . . . . . . . . . . . . . . . . . .1
Bedienungsanleitung . . . . . . . . . . . . . . . .17
Mode d’emploi . . . . . . . . . . . . . . . . . . . .33
Manual Usuario . . . . . . . . . . . . . . . . . . . .49
Instruzioni d’impiego . . . . . . . . . . . . . . . .65
Specifications . . . . . . . . . . . . . . . . . . . . .81
mLINE®

m10 m20 m100 m200 m1000 m5000
1726051 726052 726052 726052 726053 726054
2726041 726041 726041 726041 726042 N/A
3731084 726083 726042 726039 726040 N/A
4N/A N/A N/A N/A N/A 726038
5731076 726084 726034 726035 726036 726037
6N/A N/A N/A N/A N/A N/A
7726006 726006 726006 726006 726006 726006
8726022 726082 726026 726027 726028 726029
9726086 726087 726088 726089 726090 726091
10 726005 726005 726005 726005 726005 726005
11 726004 726004 726004 726004 726004 726004
12 726003 726003 726003 726003 726003 726003
13 726002 726002 726002 726002 726002 726002
14 726001 726001 726001 726001 726001 726001
Parti di ricambio
Collare dell’espulsore
puntale
Contenitore cono
Cono ancoraggio
puntali completo
Cilindro cono
Pistone
Sigilla pistone
Copri dado di
calibrazione
Display
Impugnatura
Espulsore puntali
Finestra ID
Etichetta ID
Collare
dell’impugnatura
Cappuccio
(set di 5 colori)
Spare Parts
1Tip ejector collar
2Tip cone holder
3Tip cone
complete
4Tip cone
cylinder
5Piston
6Piston seal
7Calibration
nut cover
8Display
9Handle
10 Tip ejector
11 ID window
12 ID tag
13 Handle collar
14 Cap
(set of 5 colours)
Ersatzteile
Spitzenabwurf
Konusfilterhalter
Spitzenkonus
Konuszylinder
Kolben
Kolbenstempel
Abdeckung
Kalibrierschraube
Display
Handgriff
Spitzenabwerfer
ID Fenster
ID Aufkleber
Griffhalter
Abdeckungen
(5 Farben Set)
Pièces détachées
Tube d’éjection
Bague de retenue
Porte-cône
complet
Cylindre
Piston
Joint de piston
Dessus d’écrou
Affichage
Ejecteur
Corps de pipette
Corps d’identification
Clip d’identification
Poignet
Bouchons
(5 couleurs)
Repuestos
Expulsor de puntas
Anillo soporte
de puntas
Soporte de puntas
completo
Cilindro del soporte
de puntas
Pistón
Junta de pistón
Tapa del mecanismo
de calibración
Display
Empuñadura
Sistema expulsor
de puntas
Visor ID
Etiqueta de
identificación
Cubierta de la
empuñadura
Tapas
(set de 5 colores)
14 13
11
12
10
9
7
8
3
5
1
2
m10, m20,
m100 m200, m1000 m5000
14 13
11
12
10
9
7
8
3
5
1
2
14 13
11
12
10
9
7
8
5
1
6
4
6

m10/8 m100/8 m300/8 m10/12 m100/12 m300/12
1726131 726132 726133 726134 726135 726136
2726137 726138 726138 726139 726140 726140
3726149 726150 726151 726149 726150 726151
4726146 726147 726148 726146 726147 726148
5726152 726153 726154 726152 726153 726154
6726006 726006 726006 726006 726006 726006
7726022 726141 726142 726022 726141 726142
8726143 726144 726145 726143 726144 726145
9726005 726005 726005 726005 726005 726005
10 726004 726004 726004 726004 726004 726004
11 726003 726003 726003 726003 726003 726003
12 726002 726002 726002 726002 726002 726002
13 726001 726001 726001 726001 726001 726001
Parti di ricambio
Alloggiamento del cono
di ancoraggio del puntale
Barra di espulsione
del puntale
Cono ancoraggio
puntali completo
Pistone
Assemblaggio
del pistone
Copri dado di
calibrazione
Display
Impugnatura
Espulsore puntali
Finestra ID
Etichetta ID
Collare
dell’impugnatura
Cappuccio
(set di 5 colori)
Spare Parts
1Tip cone housing
2Tip ejector bar
3Tip cone
complete
4Piston
5Piston assembly
6Calibration
nut cover
7Display
8Handle
9Tip ejector
10 ID window
11 ID tag
12 Handle collar
13 Cap
(set of 5 colours)
Ersatzteile
Spitzenkonus-
gehäuse
Spitzenabwurfs-
balken
Spitzenkonus
Kolben
Kolbenzu-
sammensetzung
Abdeckung
Kalibrierschraube
Display
Handgriff
Spitzenabwerfer
ID Fenster
ID Aufkleber
Griffhalter
Abdeckungen
(5 Farben Set)
Pièces détachées
Module
porte-cônes
Barre
d’éjection
Porte-cône
complet
Piston
Piston
assemblé
Dessus d’écrou
Affichage
Ejecteur
Corps de pipette
Corps d’identification
Clip d’identification
Poignet
Bouchons
(5 couleurs)
Repuestos
Carcasa del
Soporte de puntas
Expulsor de
puntas
Soporte de puntas
completo
Pistón
Conjunto de
pistón
Tapa del mecanismo
de calibración
Display
Empuñadura
Sistema expulsor
de puntas
Visor ID
Etiqueta de
identificación
Cubierta de la
empuñadura
Tapas
(set de 5 colores)
8-channel: m10, m100, m300
12-channel: m10, m100, m300
13
11
12
10
9
7
8
3
5
1
2
6
4

CONTENTS
1. Product description 2
2. Biohit tips 4
3. Unpacking 4
4. Personal identification 4
5. Pipettor holder and carousel 5
5.1. Installing the pipettor holder 5
5.2. Carousel stand 5
6. Calibration tool (also tube and ID window opener) 5
7. Operating the pipettor 6
7.1. Setting the volume 6
7.2. Sealing and ejecting the tips 6
7.3. Protective tip cone filters and filter ejection 7
8. Pipetting techniques 8
8.1. Forward pipetting 8
8.2. Reverse pipetting 8
8.3. Repetitive reverse technique 9
9. Recommendations for good pipetting 9
10. Maintenance 10
10.1. Daily cleaning of the outer surface of the pipettor 10
10.2. Cleaning and decontaminating the lower part of the pipettor 10
10.3. Sterilization of the pipettor 11
11. Testing the performance and recalibration 12
11.1. Testing the performance 12
11.2. Recalibration 14
12. Troubleshooting 15
13. Warranty information 16
14. Performance specifications 16
1

1. Product description
Your new Biohit mLINE is an autoclavable air displacement pipettor.
The attractive and ergonomical design of the mLINE pipettor together
with its very low pipetting forces reduce the risk of repetitive strain
injuries (RSI)1. The pipettor is designed for both right- and left-handed
use.
The special tip cone design of the pipettor offers the possibility to use
replaceable Safe-Cone filters to help prevent the contamination and
damage of the pipettor. The operating button of the mLINE allows safe
and easy filter ejection. The pipettor uses disposable tips.
Biohit mLINE single- and multichannel pipettors
1. Note: It is knowledge that prolonged pipetting can cause RSI. The
manufacturer is not responsible for RSI or any related diseases caused
by prolonged pipetting.
2
Safe Cone filters
Colour Volume 50 pcs/bag
Cat. No. Code Channels Range Increment Tip Standard Plus
725020 Grey 1-ch 0.5 - 10 µl 0.01 µl 10 N/A N/A
725030 Yellow 1-ch 2 - 20 µl 0.02 µl 300 721014 N/A
725050 Yellow 1-ch 10 - 100 µl 0.1 µl 300, 350 721008 721018
725060 Yellow 1-ch 20 - 200 µl 0.2 µl 300, 350 721007 721017
725070 Blue 1-ch 100 - 1000 µl 1 µl 1000 721006 721016
725080 Green 1-ch 500 - 5000 µl 10 µl 5000 721005 721015
725120 Grey 8-ch 0.5 - 10 µl 0.01 µl 10 N/A N/A
725130 Yellow 8-ch 5 - 100 µl 0.1 µl 300, 350 721008 721018
725140 Orange 8-ch 30 - 300 µl 0.2 µl 350 721007 721017
725220 Grey 12-ch 0.5 - 10 µl 0.01 µl 10 N/A N/A
725230 Yellow 12-ch 5 - 100 µl 0.1µl 300, 350 721008 721018
725240 Orange 12-ch 30 - 300 µl 0.2 µl 350 721007 721017

Pipettor Description
3
Cap .......................
Operating
button ...................
Tip
ejector .......
Display .............
Volume
lock ..................
Colour
coding...............
Tip ejector
collar ......................
Tip cone....................
ID window
and tag
Finger
support
......Handle
.. Calibration
nut
Safe Cone
........ filter
........
......
Tip cone .........
......Tip cone
housing
Tip
......ejector bar
.......Safe Cone
filter

2. Biohit tips
The full range of Biohit pipettor tips are recommended for use with
Biohit mLINE pipettors. Biohit standard tips are made of virgin
polypropylene. Biohit also offers a full range of filter tips. Biohit
standard tips are available as bulk packages, space saving refill
systems and autoclavable (121°C, 20 min, 1 atm) trays. Pre-sterilized
tips in trays are also available. (Fig. 1.)
Fig. 1.
3. Unpacking
The Biohit mLINE pipettor package contains the following items:
1. Pipettor
2. Tip
3. Caps for colour coding
4. Calibration tool (also tube and ID window opener)
5. Pipettor holder
6. Identification tags
7. Safe-Cone filters (pipettors >10 µl)
8. Grease
9. Instruction manual
10. Performance certification in accordance with ISO 8655-6
Please check that all items are included and that no damage has
occurred during shipment.
4. Personal identification
Each Biohit mLINE pipettor can be identified with a tag located under
the ID window which is placed on the finger support.
1. Remove the ID window by using the calibration tool (Chapter 6.).
2. Remove the tag and mark the identifying information on it.
3. Position the tag on place and clip the window back into place.
4

A
B
C
5
5. Pipettor holder and carousel stand
For convenience and safety always keep the pipettor vertically on its
own holder or carousel stand when not in use.
Cat.No. Product
725600 Biohit
mLINE Carousel Stand
725610 Biohit
mLINE Pipettor Holder
5.1. Installing the pipettor holder
1. Clean the shelf surface with ethanol.
2. Remove the protective paper from
the adhesive tape.
3. Install the holder by pressing it
against the edge of the shelf (Fig. 2).
4. Place the pipettor onto the holder.
Fig. 2.
5.2. Carousel stand
A convenient and ergonomical
Carousel Stand (Fig. 3) for six
Biohit mLINE pipettors is also available.
Fig. 3.
6. Calibration tool (also tube and ID window opener)
The calibration tool is designed for the following purposes (Fig. 4):
1. Tool for recalibration (A).
2. Opening tool for the ID window and lid of the calibration nut (B).
3. Opening tool for the tubes (C).
Fig. 4.

7. Operating the pipettor
7.1. Setting the volume
The volume of the pipettor is clearly shown through the display. The
volume setting is carried out with the following steps (Fig. 5):
1. Press the volume lock and hold it down.
2. Set the volume by rotating the operating button (clockwise to
decrease the volume and counterclockwise to increase).
3. Release the volume lock.
Fig. 5 Volume Lock
+
–
Note: Do not rotate the operating button without pressing down the
volume lock. Do not rotate the operating button outside the volume
range of the pipettor (Chapter. 1.)
7.2. Sealing and ejecting tips
To ensure the maximum accuracy and precision Biohit tips are
recommended to be used with mLINE pipettors. Before fitting a tip
ensure that the pipettor tip cone is clean. Press the tip on the tip cone
of the pipettor. The pipettor is provided with a spring loaded tip cone
for optimal sealing.
Eject the tip by pressing the tip ejector with your thumb (Fig. 6). Make
sure that the tip is disposed into a suitable waste container.
Fig. 6. Tip ejector
Note: If the tip is pressed too hard the tip cone yields and, as a result,
the counter and operating button moves.
6

7.3. Protective tip cone filters and filter ejection
The tip cone design of the Biohit mLINE pipettors (> 10 µl) allows the
use of Biohit Safe-Cone filters in the tip cones. These removable filters
prevent liquids and aerosols from entering the pipettor and, thus,
protects the pipettor from contamination and damage.
The Safe-Cone filters are available as Standard or Plus versions. It is
recommended to use the Standard filter for general applications and
the Plus filter for more demanding applications such as cell culture,
bacterial and virological work and molecular biology. Filters need to be
changed regularly. The interval for filter changing is application
dependant but the recommendation is to change the filter daily (after 50
– 250 pipetting cycles) and always in case of over-aspiration. (See
Chapter 1 for ordering information.)
The Safe-Cone filters can be removed with the unique filter ejector
without touching the filters by hand. Remove the operating button cap
and eject the filter by pressing the operating button down (Fig. 7). Make
sure that the filter is disposed into a suitable waste container. Clean the
tip cone if needed and put the new filter in place.
Fig. 7.
7

8
8. Pipetting techniques
Two basic pipetting techniques, forward and reverse pipetting, are
associated with mLINE pipettors. Forward pipetting is the most
common used pipetting technique. The technique employs the blow-
out function ensuring complete delivery of the liquid. Reverse
pipetting is recommended for highly viscous, biological or foaming
liquids, or very small volumes of liquid. A selected volume plus an
excess is aspirated into the tip. The delivery is done without blow-out,
and, thus, the excess volume remains in the tip. The reverse technique
also facilitates the repeated delivery of the same volume.
8.1. Forward pipetting (Fig. 8)
1. Fit the tip onto the pipettor tip cone.
2. Press the operating button to the first stop.
3. Place the tip just under the surface of the liquid (2-3 mm) and
smoothly release the operating button up to the starting position.
Wait one second. Carefully withdraw the tip from the liquid,
touching against the edge of the container to remove excess from
the outside of the tip.
4. Dispense the liquid by pressing the operating button to the first
stop. After a short delay press the operating button to the second
stop. This action will empty the tip.
Fig. 8. 0
1
2
8.2. Reverse pipetting (Fig. 9)
1. Fit the tip onto the pipettor tip cone.
2. Press the operating button all the way to the second stop.
3. Place the tip just under the surface of the liquid (2–3 mm) and
smoothly release the operating button up to the starting position.
4. Withdraw the tip from the liquid touching against the edge of the
container to remove excess.
5. Press the operating button smoothly to the first stop to deliver the
present volume. Hold the operating button at the first stop. The
liquid that remains in the tip should not be included in the delivery.
6. Discard the remaining liquid by pressing the operating button to
the second stop.
Fig. 9. 0
1
2

8.3. Repetitive reverse technique
1. Follow the reverse technique steps 1 to 5.
2. Continue pipetting by repeating steps 3 to 5 as long as needed.
3. Finally discard the remaining liquid by pressing the operating
button to the second stop.
9. Recommendations for good pipetting
• Make sure that the tip is firmly attached to the tip cone.
• Hold the pipettor vertically when aspirating the liquid and place the
tip only a few millimeters into the liquid.
• Always control the operating button slowly and smoothly.
• Pre-rinse the tip before aspirating the liquid by filling and emptying
the tip for three to five times. This is important especially when
pipetting liquids with a viscosity and density greater than water or
liquids with high vapor pressure (e.g. ethanol).
• Check that the pipettor, tip and liquid are at the same temperature.
• When pipetting liquids with temperatures different to the ambient
temperature change the tip after each pipetting. Do not pre-rinse
the tip.
• To avoid contamination, do not rest the pipettor on its side
especially with the tip attached.
• Change the tip cone filter regularly (recommendation after 50 – 250
pipetting cycles).
• Never strike the tip cone against the tip tray when mounting the tip
as this can damage the pipettor.
• Avoid rough handling and do not drop the pipettor.
• Avoid exposing the unit to extreme temperature changes, humidity
and dust (operating temperature from 15ºC to 40ºC).
9

10. Maintenance
Biohit mLINE pipettors have been designed for easy in-house service.
If the pipettor is used daily it is recommended to clean/decontaminate
and check the performance of the pipettor every three months. Biohit
also provides complete repair and recalibration service for your pipettor
including a service report and performance certificate.
Note: Please make sure that the pipettor has been decontaminated
before you send it to us or your local representative. Please advise if
any hazardous material has been used with your pipettor.
Note: The use of the tip cone filters may prolong the interval of the
service. Change the filters regularly.
Note: It is recommended to always use gloves when cleaning the
pipettor.
10.1. Daily cleaning of the outer surface of the pipettor
Your Biohit mLINE pipettor should be checked every day for
cleanliness. To clean and decontaminate the outer surface of your
pipettor use Biohit Proline‚ Biocontrol (Cat.no. 724004, 5 litres) and a
soft lint-free cloth. It is also possible to use ethanol (70%), isopropanol
(60%) or a mild detergent as a cleaning agent.
Gently clean the surface of the pipettor with moistened cloth and wipe
dry. Pay special attention to the tip cone. Change the tip cone filter if
needed (Chapter 7.3.)
10.2. Cleaning and decontaminating the lower part of the pipettor
If your pipettor is in daily use it is recommended to clean/decontaminate
and grease the pipettor every three months. It is recommended to send
the multichannel pipettors to your local Biohit service representative for
cleaning and greasing. To clean and decontaminate the lower parts of
the single channel pipettor, follow these steps:
Disassembling and cleaning (see the pictures under the cover page):
1. Eject the tip cone filter (if fitted, Chapter 7.3.)
2. Unscrew the tip ejector collar (1) counterclockwise and remove it.
3. Pipettors m10,m20, m100, m200, m1000:
Unscrew the tip cone holder (2) counterclockwise and carefully
remove it with the tip cone (3).
Pipettor m5000:
Unscrew the tip cone cylinder (4) counterclockwise and remove it.
4. Clean the tip ejector collar, the tip cone holder, the tip cone
(cylinder) and the piston (5) with Biohit Proline‚ Biocontrol,
ethanol (70%), isopropanol (60%) or mild detergent and soft
lint-free cloth.
5. Clean the interior of the tip ejector collar and the tip cone
(cylinder) with a cotton swab. Be careful with the pipettors m10,
m20 and m100 so that the seal inside the tip cone will not
damage.
6. Rinse the parts with distilled water if needed and let the parts
dry.
10

7. Pipettors m10, m20 and m100:
Put a thin layer of grease on the piston (5).
Pipettors m200 and m1000:
Put a thin layer of grease around the seal (6).
Pipettor m5000:
Put a thin layer of grease on the seal (6) and the interior of the tip
cone cylinder (4).
Note: Avoid excess grease. Use only the grease provided with the
pipettor.
Note: Before reassembling check that no lint or particles are on the
surface of the piston.
Decontamination:
For complete decontamination of the lower parts place the tip ejector
collar (1), tip cone holder (2), tip cone (3) and tip cone cylinder (4) (only
the model m5000) into a beaker containing Biohit Proline‚ Biocontrol
and leave for at least 30 minutes. Wipe the piston with Biohit Proline‚
Biocontrol and the lint-free cloth. Rinse the parts with distilled or sterile
water. Let the parts dry. Grease the piston and seal according to the
instructions given earlier in this chapter.
Reassembling:
1. Pipettors m10, m20, m100, m200, m1000:
Carefully place the tip cone (3) on the piston and attach it by
screwing the tip cone holder (2) clockwise.
Pipettor m5000:
Carefully place the tip cone cylinder (4) on the piston and screw
clockwise.
Make sure that the tip cone (cylinder) is properly tightened.
Avoid over tightening.
2. Attach the tip ejector collar (1) by screwing it clockwise.
3. Place the new tip cone filter on its place.
4. Press the operating button several times to ensure that the grease
has spread evenly.
5. Check the performance of the pipettor.
Note: It is always necessary to check the performance of the pipettor
after in-house service or maintenance.
10.3. Sterilization of the pipettor
The entire mLINE pipettor can be sterilized by steam autoclaving at
121OC, (252OF), 1 atm for 20 minutes. Remove the tip cone filter (if fitted,
Chapter 7.3). The single channel pipettors can be autoclaved without
special preparations. Unscrew the
lower part of the multichannel pi-
pettors by holding the connecting
collar and turning the lower part
360° counterclockwise (Fig. 10).
Put the pipettor into the sterilization
bag and place it into the autoclave.
After autoclaving the pipettor must
11
360°
Fig. 10
Connecting
collar

be cooled down and left to dry overnight before use. Screw the lower
part of the multichannel pipettors by holding the connecting collar and
turning the lower part 360° clockwise. Make sure that the lower part is
properly tightened. It is recommended to check the performance of the
pipettor after every autoclaving. It is also recommended to grease the
piston/seal of the pipettor after every 10 autoclaving.
11. Testing the performance and recalibration
It is recommended to check the performance of your Biohit mLINE
pipettors regularly (e.g. every 3 months) and always after in-house
maintenance. However, the user should establish a regular testing
routine for their pipettors with regard to accuracy requirements of the
application, frequency of use, number of operators using the pipettor,
nature of the liquid dispensed and the acceptable maximum
permissible errors established by the user. (ISO 8655-1.)
11.1. Testing the performance
Performance testing should take place in a draught-free room at 15 –
30OC, constant to +/- 0.5OC and humidity above 50%. The pipettor, tips
and the test water should have stood in the test room a sufficient time
(at least 2 hours) to reach equilibrium with the room conditions. Use
distilled or deionised water (grade 3, ISO 3696). Use an analytical
balance with a readability of 0.01 mgs. (ISO 8655-6.)
Weighing
1. Adjust the desired test volume Vs.
2. Carefully fit the tip onto the tip cone.
3. Fill the tip with test water and expel to waste five times to reach a
humidity equilibrium in the dead air volume.
4. Replace the tip. Pre-wet the tip by filling it once with test water and
expel to waste.
5. Aspirate the test water, immersing the tip only 2-3 mm below the
surface of the water. Keep the pipettor vertical.
6. Withdraw the pipettor vertically and touch the tip against the inside
wall of the test water container.
7. Pipette the water into the weighing vessel, touching the tip against
the inside wall of the vessel just above the liquid surface at an
angle of 30° to 45°. Withdraw the pipettor by drawing the tip 8-10
mm along the inner wall of the weighing vessel.
8. Read the weight in mgs (mi).
9. Repeat the test cycle until 10 measurements have been recorded.
10. Convert the recorded masses (mi) to volumes (Vi)
Vi= miZ Z = correction factor (Table 1)
11. Calculate the mean volume (V) delivered:
V = (∑Vi)/10
12. For conformity evaluation calculate the systematic error esof the
measurement:
in µl: es= V – VsVs= selected test volume
or in %: es= 100 (V – Vs)/Vs
12

13. For conformity evaluation calculate the random error of the
measurement:
as standard deviation s = ∑(Vi– V)2n = number of
measurement (10)
n – 1
or as coefficient of variation CV = 100s/V
14. Compare the systematic error (inaccuracy) and random error
(imprecision) with the values in the performance specifications
(page 81) or the specifications of your own laboratory.
If the results fall within the specifications, the pipettor is ready for
use. Otherwise check both systematic and random errors and,
when necessary, proceed to the recalibration procedure
(Chapter 11.2).
Note: Systematic error (inaccuracy) is the difference between the
dispensed volume and the selected test volume. Random error
(imprecision) is the scatter of the dispensed volumes around the mean
of the dispensed volume. (ISO 8655-1.)
Note: Biohit specifications are achieved in strictly controlled conditions
(ISO 8655-6). The user should establish own specifications based on
the field of use and the accuracy requirements placed on the pipettor
(ISO8655-1).
Table 1
Note: This method is based on ISO 8655.
Z-values (µl/mg):
Temp. (°C) Air Pressure (kPa)
95 100 101.3 105
20.0 1.0028 1.0028 1.0029 1.0029
20.5 1.0029 1.0029 1.0030 1.0030
21.0 1.0030 1.0031 1.0031 1.0031
21.5 1.0031 1.0032 1.0032 1.0032
22.0 1.0032 1.0033 1.0033 1.0033
22.5 1.0033 1.0034 1.0034 1.0034
23.0 1.0034 1.0035 1.0035 1.0036
23.5 1.0036 1.0036 1.0036 1.0037
13

14
11.2. Recalibration
The calibration of your mLINE pipettor has been factory checked and
certified at 22OC using grade 3 distilled water according to ISO 3696.
The calibration is based on ISO 8655-6: Gravimetric test method for
volumetric instruments. The pipettor’s specifications are guaranteed
only with genuine Biohit tips. If you find the pipettor to be inaccurate
after performance testing, please follow the instructions below:
1. Remove the lid of the calibration nut, located at the backside of the
handle, with the aid of the calibration tool (Fig. 11).
2. Place the hexagonal head of the calibration tool into the hole of the
calibration nut.
3. Turn the adjustment lock counterclockwise to decrease and
clockwise to increase the volume.
4. Repeat testing the performance procedure (Chapter 11.1.).
Continue until the results are correct.
Note: Biohit offers accredited calibration service. Please contact your
local Biohit representative for further information.
Fig. 11.
–
+

12. Trouble shooting
Symptom Possible cause Solution
Droplets left inside Unsuitable tip Use original tips
the tip
Leakage or pipetted Non-uniform wetting Attach new tip
volume too small of the plastic
Tip incorrectly Attach firmly
attached
Unsuitable tip Use original tips
Foreign particles Clean the tip cone,
between tip and attach new tip
tip cone
Tip cone holder Tighten the tip
incorrectly tightened cone holder
Pipettor damaged Return to your
Biohit service
representative
for servicing
Pipettor out of Incorrect operation Follow instructions
established
specifications Unsuitable tip Use original tips
Calibration altered Recalibrate
Operating button Liquid has penetrated Clean and grease
jammed or moves tip cone and dried the piston/seal
erratically and the tip cone
Safe Cone filter has Change filter
been contaminated
Insufficient amount of Grease accordingly
grease on a piston
and seal
Tip ejector jammed Tip ejector collar has Remove and clean
or moves erratically been contaminated the ejector collar
and tip cone
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13. Warranty information
The Biohit mLINE pipettors are covered by warranty for 3 years against
defects in materials and workmanship. Should your mLINE pipettor fail
to function at any time, please contact your local Biohit representative.
ANY WARRANTY WILL, HOWEVER, BE DEEMED AS VOID IF FAULT
IS FOUND TO HAVE BEEN CAUSED BY MALTREATMENT, MISUSE,
UNAUTHORIZED MAINTENANCE OF SERVICE OR NEGLIGENCE
OF REGULAR MAINTENANCE AND SERVICE, ACCIDENTAL
DAMAGE, INCORRECT STORAGE OR USE OF THE PRODUCTS
FOR OPERATIONS OUTSIDE THEIR SPECIFIED LIMITATIONS,
OUTSIDE THEIR SPECIFICATIONS, CONTRARY TO THE
INSTRUCTIONS GIVEN IN THIS MANUAL OR WITH OTHER THAN
THE MANUFACTURER’S ORIGINAL TIPS.
Each Biohit mLINE pipettor is tested before shipping by the
manufacturer. The Biohit Quality Assurance Procedure guarantees that
the Biohit mLINE pipettor you have purchased is ready for use.
Each Biohit mLINE pipettor is CE marked.
14. Performance specifications
The manufacturer’s specifications (page 81) are guaranteed only when
the manufacturer’s original tips are used. The manufacturer’s
specifications below should be used as guidelines when establishing
your own performance specifications in accordance with ISO 8655.
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