Biohit mLINE m3 User manual

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mLINE®
Instruction Manual
Bedienungsanleitung
Mode d’ emploi
Manual Usuario
Instruzioni d’impiego
Инструкция пользователя
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m3 m10 m20 m100 m200 m1000 m5000 m10 ml
1 726051 726051 726052 726052 726052 726056 726054 726055
2 726041 726041 726041 726041 726041 726042 N/A N/A
3 726044 731084 726083 726042 726039 726048 N/A N/A
4 N/A N/A N/A N/A N/A N/A 726038 726047
5 726032 731076 726084 726034 726035 726036 726037 726031
6 N/A N/A N/A N/A N/A N/A N/A N/A
7 726006 726006 726006 726006 726006 726006 726006 726006
8 726021 726022 726082 726026 726027 726028 726029 726012
9 726012 726012 726012 726012 726012 726012 726012 726012
10 726005 726005 726005 726005 726005 726005 726005 726005
11 726004 726004 726004 726004 726004 726004 726004 726004
12 726003 726003 726003 726003 726003 726003 726003 726003
13 726002 726002 726002 726002 726002 726002 726002 726002
14 726001 726001 726001 726001 726001 726001 726001 726001
14 13
11
12
10
9
7
8
3
5
1
2
m3, m10,
m20, m100 m200, m1000 m5000, m10 ml
14 13
11
12
10
9
7
8
3
5
1
2
14 13
11
12
10
9
7
8
5
1
6
4
6
Spare Parts
1Tip ejector
collar
2Tip cone
holder
3Tip cone
complete
4Tip cone
cylinder
5Piston
6Piston seal
7Calibration
nut cover
8Display
9Handle
10 Tip ejector
11 ID window
12 ID tag
13 Handle collar
14 Cap (set of
5 colours)
Ersatzteile
Spitzenabwurf
Konusfilterhalter
Spitzenkonus
Konuszylinder
Kolben
Kolbenstempel
Abdeckung
Kalibrierschraube
Display
Handgriff
Spitzenabwerfer
ID Fenster
ID Aufkleber
Griffhalter
Abdeckungen
(5 Farben Set)
Piéces détachées
Tube d’éjection
Bague de retenue
Porte-cône
complet
Cylindre
Piston
Joint de piston
Dessus d’écrou
Affichage
Ejecteur
Corps de pipette
Corps d’identification
Clip d’identification
Poignet
Bouchons
(5 couleurs)
Repuestos
Expulsor de puntas
Anillo soporte de
puntas
Soporte de puntas
completo
Cilindro del soporte
de puntas
Pistón
Junta de pistón
Tapa del mecanismo
de calibración
Display
Empuñadura
Sistema expulsor
de puntas
Visor ID
Etiqueta de
identificación
Cubierta de la
empuñadura
Tapas
(set de 5 colores)
Parti di ricambio
Collare dell’espulsore
puntale
Contenitore cono
Cono ancoraggio
puntali completo
Cilindro cono
Pistone
Sigilla pistone
Copri dado di
calibrazione
Display
Impugnatura
Espulsore puntali
Finestra ID
Etichetta ID
Collare
dell’impugnatura
Cappuccio
(set di 5 colori)
Запасная часть
Сбрасыватель
наконечников
Муфта крепления
посадочного конуса
Посадочный конус
всборе
Посадочный конус
Поршень
Уплотнительная
манжета
Узел калибровки
Крышка дисплея
Рукоять
Сбрасыватель
наконечников
Прозрачная
защитная пластинка
Идентификационный
вкладыш
Воротник рукояти
Декоративный
съемный колпачок
(5 цветов)
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m10/8 m100/8 m300/8 m10/12 m100/12 m300/12
1 726131 726132 726133 726134 726135 726136
2 726137 726138 726138 726139 726140 726140
3 726149 726150 726151 726149 726150 726151
4 726146 726147 726148 726146 726147 726148
5 726152 726153 726154 726152 726153 726154
6 726006 726006 726006 726006 726006 726006
7 726022 726141 726142 726022 726141 726142
8 726012 726012 726012 726012 726012 726012
9 726005 726005 726005 726005 726005 726005
10 726004 726004 726004 726004 726004 726004
11 726003 726003 726003 726003 726003 726003
12 726002 726002 726002 726002 726002 726002
13 726001 726001 726001 726001 726001 726001
8-channel: m10, m100, m300
12-channel: m10, m100, m300
13
11
12
10
9
7
8
3
5
1
2
6
4
Spare Parts
1 Tip cone
housing
2 Tip ejector bar
3 Tip cone
complete
4 Piston
5 Piston
assembly
6 Calibration
nut cover
7 Display
8 Handle
9 Tip ejector
10 ID window
11 ID tag
12 Handle collar
13 Cap
(set of 5
colours)
Ersatzteile
Spitzenkonus-
gehäuse
Spitzenabwurfs-
balken
Spitzenkonus
Kolben
Kolbenzu-
sammensetzung
Abdeckung
Kalibrierschraube
Display
Handgriff
Spitzenabwerfer
ID Fenster
ID Aufkleber
Griffhalter
Abdeckungen
(5 Farben Set)
Piéces détachées
Module
porte-cônes
Barre d’éjection
Porte-cône
complet
Piston
Piston
assemblé
Dessus d’écrou
Affichage
Ejecteur
Corps de pipette
Corps
d’identification
Clip d’identification
Poignet
Bouchons
(5 couleurs)
Repuestos
Carcasa del
Soporte de puntas
Expulsor de
puntas
Soporte de puntas
completo
Pistón
Conjunto de
pistón
Tapa del mecanismo
de calibración
Display
Empuñadura
Sistema expulsor
de puntas
Visor ID
Etiqueta de
identificación
Cubierta de la
empuñadura
Tapas
(set de 5 colores)
Parti di ricambio
Alloggiamento del
cono di ancorag-
gio del puntale
Barra di espul-
sione del puntale
Cono ancoraggio
puntali completo
Pistone
Assemblaggio
del pistone
Copri dado di
calibrazione
Display
Impugnatura
Espulsore puntali
Finestra ID
Etichetta ID
Collare
dell’impugnatura
Cappuccio
(set di 5 colori)
Запасная часть
Корпус поршневой
части
Сбрасыватель
наконечника
Посадочный конус
Поршень
Поршень системы
всборе
Узел калибровки
Крышка дисплея
Рукоять
Сбрасыватель
наконечников
Прозрачная
защитная пластинка
Идентификационный
вкладыш
Воротник рукояти
Декоративный
съемный колпачок
(5 цветов)
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Instruction Manual .......................................1
Bedienungsanleitung..................................17
Mode d’emploi ...........................................33
Manual Usuario ..........................................49
Instruzioni d’impiego ..................................65
Инструкция пользователя ..............................81
Specifications.............................................97
mLINE®
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1
CONTENTS
1. INTENDED USE ........................................................... 2
2. PRODUCT DESCRIPTION.......................................... 2
3. BIOHIT OPTIFIT TIPS.................................................. 4
4. UNPACKING ................................................................ 4
5. PERSONAL IDENTIFICATION.................................... 4
6. PIPETTE HOLDER AND CAROUSEL STAND........... 5
6.1. Installing the pipette holder................................ 5
6.2. Carousel stand ................................................... 5
7. CALIBRATION TOOL
(also tube and ID window opener)............................ 5
8. OPERATING THE PIPETTE ........................................ 6
8.1. Setting the volume.............................................. 6
8.2. Sealing and ejecting tips.................................... 6
8.3. Protective tip cone filters.................................... 7
9. PIPETTING TECHNIQUES.......................................... 7
9.1. Forward pipetting (Fig. 5) ................................... 8
9.2. Reverse pipetting (Fig. 9).................................... 8
9.3. Repetitive reverse technique.............................. 9
10. RECOMMENDATIONS FOR GOOD PIPETTING ...... 9
11. MAINTENANCE ........................................................... 9
11.1. Daily cleaning of the outer surface
of the pipette..................................................... 10
11.2. Cleaning and decontaminating
the lower part of the pipette............................. 10
11.3. Sterilization of the pipette .................................11
12. TESTING THE PERFORMANCE AND
RECALIBRATION....................................................... 12
12.1. Testing the performance .................................... 12
12.2. Recalibration..................................................... 14
13. TROUBLE SHOOTING .............................................. 15
14. WARRANTY INFORMATION .................................... 16
15. PERFORMANCE SPECIFICATIONS ........................ 16
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2
1. INTENDED USE
This liquid handling device is designed and manufactured to be
used with the IVD-product as an IVD-accessory and as a stand
alone laboratory equipment.
2. PRODUCT DESCRIPTION
Your new Biohit mLINE is an autoclavable air displacement
pipette. The attractive and ergonomical design of the mLINE
pipette together with its very low pipetting forces reduce the risk
of repetitive strain injuries (RSI)1. The pipette is designed for both
right- and left-handeduse
The special tip cone design of the pipette offers the possibility to
use replaceable Safe-Cone filters to help prevent the contamination
and damage of the pipette. The operating button of the mLINE
allows safe and easy filter ejection. The pipette uses disposable
tips.
Biohit mLINE single- and multichannel pipettes
1. Note: It is knowledge that prolonged pipetting can cause RSI.
The manufacturer is not responsible for RSI or any related diseases
caused by prolonged pipetting.
Safe Cone filters
Colour Volume 50 pcs/bag
Cat. No. Code Channels Range Increment Tip Standard Plus
725010 Grey 1-ch 0.1 - 3 μl 0.002 μl 10 N/A N/A
725020 Grey 1-ch 0.5 - 10 μl 0.01 μl 10 N/A N/A
725030 Yellow 1-ch 2 - 20 μl 0.02 μl 300 721014 N/A
725050 Yellow 1-ch 10 - 100 μl 0.1 μl 300, 350 721008 721018
725060 Yellow 1-ch 20 - 200 μl 0.2 μl 300, 350 721007 721017
725070 Blue 1-ch 100 - 1000 μl 1 μl 1000 721006 721016
725080 Green 1-ch 500 - 5000 μl 10 μl 5000 721005 721015
725090 Red 1-ch 1 - 10 ml 20 μl 10 ml 721005 721015
725120 Grey 8-ch 0.5 - 10 μl 0.01 μl 10 N/A N/A
725130 Yellow 8-ch 5 - 100 μl 0.1 μl 300, 350 721008 721018
725140 Orange 8-ch 30 - 300 μl 0.2 μl 350 721007 721017
725220 Grey 12-ch 0.5 - 10 μl 0.01 μl 10 N/A N/A
725230 Yellow 12-ch 5 - 100 μl 0.1μl 300, 350 721008 721018
725240 Orange 12-ch 30 - 300 μl 0.2 μl 350 721007 721017
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3
Pipette Description
Cap .......................
Operating
button ...................
Tip
ejector .......
Display .............
Volume
lock ..................
Colour
coding...............
Tip ejector
collar ......................
Tip cone....................
ID window
and tag
Finger
support
........ Handle
... Calibration
nut
Safe Cone
....... filter
Tip cone ...............
........ Tip cone
housing
Tip
........ ejector bar
........ Safe Cone
filter
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4
3. BIOHIT OPTIFIT TIPS
The full range of Biohit pipette tips are recommended for use with
Biohit mLINE pipettes. Biohit standard tips are made of virgin
polypropylene. Biohit also offers a full range of Safetyspace filter
tips. Biohit standard tips are available as bulk packages, space
saving refill systems and autoclavable (121°C, 20 min, 1 atm) trays.
Pre-sterilized tips in trays are also available. (Fig. 1.)
Fig. 1.
4. UNPACKING
The Biohit mLINE pipette package contains the following items:
1. Pipette
2. Tip
3. Caps for colour coding
4. Calibration tool (also tube and ID window opener)
5. Pipette holder
6. Identification tags
7. Safe-Cone filters (pipettors >10 μl)
8. Grease
9. Instruction manual
10. Performance certification in accordance with ISO 8655-6
Please check that all items are included and that no damage has
occurred during shipment.
5. PERSONAL IDENTIFICATION
Each Biohit mLINE pipette can be identified with a tag located
underthe ID window which is placed on the finger support.
1. Remove the ID window by using the calibration tool (Chapter 7.).
2. Remove the tag and mark the identifying information on it.
3. Position the tag on place and clip the window back into
place.
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5
A
B
C
6. PIPETTE HOLDER AND CAROUSEL STAND
For convenience and safety always keep the pipette vertically on
its own holder, carousel or linear stand when not in use.
Cat.No. Product
725600 Biohit mLINE Carousel
725610 Biohit mLINE Pipette Holder
6.1. Installing the pipette holder
1. Clean the shelf surface with ethanol.
2. Remove the protective paper from
the adhesive tape.
3. Install the holder by pressing it
against the edge of the shelf (Fig. 2).
4. Place the pipette onto the holder.
6.2. Carousel stand
A convenient and ergonomical
Carousel Stand (Fig. 3) for six
Biohit mLINE pipettes is also available
7. CALIBRATION TOOL
(also tube and ID window opener)
The calibration tool is designed for the following purposes (Fig. 4):
1. Tool for recalibration (A).
2. Opening tool for the ID window and lid of
the calibration nut (B).
3. Opening tool for the tubes (C).
Fig. 4.
Fig. 2.
Fig. 3.
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6
8. OPERATING THE PIPETTE
8.1. Setting the volume
The volume of the pipette is clearly shown through the display. The
volume setting is carried out with the following steps (Fig. 5):
1. Press the volume lock and hold it down.
2. Set the volume by rotating the operating button (clockwise to
decrease the volume and counterclockwise to increase).
3. Release the volume lock.
Fig. 5. Volume Lock
+
–
Note: Do not rotate the operating button without pressing down
the volume lock. Do not rotate the operating button outside the
volume range of the pipette (Chapter. 1.)
8.2. Sealing and ejecting tips
To ensure the maximum accuracy and precision Biohit tips are
recommended to be used with mLINE pipettes. Before fitting a tip
ensure that the pipette tip cone is clean. Press the tip on the tip
cone of the pipette. The pipette is provided with a spring loaded
tip conefor optimal sealing.
Eject the tip by pressing the tip ejector with your thumb (Fig. 6).
Make sure that the tip is disposed into a suitable waste container.
Fig. 4. Tip ejector
Note: If the tip is pressed too hard the tip cone yields and, as a
result, the counter and operating button moves.
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7
8.3. Protective tip cone filters
The tip cone design of the Biohit mLINE pipettes (>10 μl) allows the
use of Biohit Safe-Cone filters in the tip cones. These removable
filters prevent liquids and aerosols from entering the pipette and,
thus, protects the pipette from contamination and damage.
The Safe-Cone filters are available as Standard or Plus versions. It
is recommended to use the Standard filter for general applications
and the Plus filter for more demanding applications such as cell
culture, bacterial and virological work and molecular biology.
Filters need to be changed regularly. The interval for filter changing
is application dependant but the recommendation is to change the
filter daily (after 50 - 250 pipetting cycles) and always in case of
over-aspiration. (See Chapter 2 for ordering information.)
The Safe-Cone filters can be removed with the unique filter ejector
without touching the filters by hand. Remove the operating button
cap and eject the filter by pressing the operating button down
(Fig. 7). Make sure that the filter is disposed into a suitable waste
container. Clean the tip cone if needed and put the new filter in
place.
Fig. 7.
9. PIPETTING TECHNIQUES
Two basic pipetting techniques, forward and reverse pipetting,
are associated with mLINE pipettes. Forward pipetting is the
most common used pipetting technique. The technique employs
the blow-out function ensuring complete delivery of the liquid.
Reverse pipetting is recommended for highly viscous, biological
or foaming liquids, or very small volumes of liquid. A selected
volume plus an excess is aspirated into the tip. The delivery is done
without blow-out, and, thus, the excess volume remains in the tip.
The reverse technique also facilitates the repeated delivery of the
same volume. If reverse pipetting technique is used the pipette
might need recalibration.
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8
9.1. Forward pipetting (Fig. 5)
1. Fit the tip onto the pipette tip cone.
2. Press the operating button to the first stop.
3. Place the tip just under the surface of the liquid (2-3 mm)
and smoothly release the operating button up to the starting
position.Wait one second. Carefully withdraw the tip from the
liquid, touching against the edge of the container to remove
excess fromthe outside of the tip.
4. Dispense the liquid by pressing the operating button to the
first stop. After a short delay press the operating button to the
second stop. This action will empty the tip.
Fig. 8. 0
1
2
9.2. Reverse pipetting (Fig. 9)
1. Fit the tip onto the pipette tip cone.
2. Press the operating button all the way to the second stop.
3. Place the tip just under the surface of the liquid (2-3 mm)
and smoothly release the operating button up to the starting
position.
4. Withdraw the tip from the liquid touching against the edge of
the container to remove excess.
5. Press the operating button smoothly to the first stop to deliver
the present volume. Hold the operating button at the first stop.
The liquid that remains in the tip should not be included in the
delivery.
6. Discard the remaining liquid by pressing the operating button
to the second stop.
Fig. 9. 0
1
2
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9
9.3. Repetitive reverse technique
1. Follow the reverse technique steps 1 to 5.
2. Continue pipetting by repeating steps 3 to 5 as long as
needed.
3. Finally discard the remaining liquid by pressing the operating
button to the second stop.
10. RECOMMENDATIONS FOR GOOD PIPETTING
• Make sure that the tip is firmly attached to the tip cone.
• Hold the pipettor vertically when aspirating the liquid and place
the tip only a few millimeters into the liquid.
• Always control the operating button slowly and smoothly.
• Pre-rinse the tip before aspirating the liquid by filling and
emptying the tip for three to five times. This is important
especially when pipetting liquids with a viscosity and density
greater than water or liquids with high vapor pressure (e.g.
ethanol).
• Check that the pipette, tip and liquid are at the same
temperature.
• When pipetting liquids with temperatures different to the
ambient temperature change the tip after each pipetting. Do
not pre-rinse the tip.
• To avoid contamination, do not rest the pipette on its side
especially with the tip attached.
• Change the tip cone filter regularly (recommendation after 50
-250 pipetting cycles).
• Never strike the tip cone against the tip tray when mounting the
tip as this can damage the pipette.
• Avoid rough handling and do not drop the pipette.
• Avoid exposing the unit to extreme temperature changes,
humidity and dust (operating temperature from 15°C to 40°C).
11. MAINTENANCE
Biohit mLINE pipettes have been designed for easy in-house
service. If the pipette is used daily it is recommended to clean/
decontaminate and check the performance of the pipette every
three months. Biohit also provides complete repair and recalibration
service for your pipette including a service report and performance
certificate.
Note: Please make sure that the pipette has been decontaminated
before you send it to us or your local representative. Please advise
if any hazardous material has been used with your pipette.
Note: The use of the tip cone filters may prolong the interval of the
service. Change the filters regularly.
Note: It is recommended to always use gloves when cleaning the
pipette.
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10
11.1. Daily cleaning of the outer surface of the pipette
Your Biohit mLINE pipette should be checked every day for
cleanliness. To clean and decontaminate the outer surface of your
pipette use Biohit Proline Biocontrol (Cat.no. 724004, 5 litres)
and a soft lint-free cloth. It is also possible to use ethanol (70%),
isopropanol (60%) or a mild detergent as a cleaning agent.
Gently clean the surface of the pipette with moistened cloth and
wipe dry. Pay special attention to the tip cone. Change the tip cone
filter if needed (Chapter 8.3.)
11.2. Cleaning and decontaminating the lower part
of the pipette
If your pipette is in daily use it is recommended to clean/
decontaminate and grease the pipette every three months. It is
recommended to send the multichannel pipettes to your local
Biohit service representative for cleaning and greasing. To clean
and decontaminate the lower parts of the single channel pipette,
follow these steps:
Disassembling and cleaning
(see the pictures under the cover page):
1. Eject the tip cone filter (if fitted, Chapter 8.3.)
2. Unscrew the tip ejector collar (1) counterclockwise and remove it.
3. Pipettes m3, m10, m20, m100, m200, m1000:
Unscrew the tip cone holder (2) counterclockwise and carefully
remove it with the tip cone (3).
Pipettes m5000 and m10 000:
Unscrew the tip cone cylinder (4) counterclockwise and remove it.
4. Clean the tip ejector collar, the tip cone holder, the tip cone
(cylinder) and the piston (5) with Biohit Proline Biocontrol,
ethanol (70%), isopropanol (60%) or mild detergent and soft
lint-free cloth.
5. Clean the interior of the tip ejector collar and the tip cone
(cylinder) with a cotton swab. Be careful with the pipettes m3,
m10, m20 and m100 so that the seal inside the tip cone will not
damage.
6. Rinse the parts with distilled water if needed and let the parts
dry.
7. Pipet tes m3, m10, m20 and m100:
Put a thin layer of grease on the piston (5).
Pipettes m200 and m1000:
Put a thin layer of grease around the seal (6).
Pipettes m5000 and m10 000:
Put a thin layer of grease on the seal (6) and the interior of the
tip cone cylinder (4).
Note: Avoid excess grease. Use only the grease provided with
the pipette.
Note: Before reassembling check that no lint or particles are on
the surface of the piston.
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11
Decontamination:
For complete decontamination of the lower parts place the tip
ejector collar (1), tip cone holder (2), tip cone (3) and tip cone
cylinder (4) (only the models m5000 and m10 000) into a beaker
containing Biohit Proline‚ Biocontrol and leave for at least 30
minutes. Wipe the piston with Biohit Proline‚ Biocontrol and the
lint-free cloth. Rinse the parts with distilled or sterile water. Let the
parts dry. Grease the piston and seal according to the instructions
given earlier in this chapter.
Reassembling:
1. Pipettes
m3, m10, m20, m100, m200, m1000:
Carefully place the tip cone (3) on the piston and attach it by
screwing the tip cone holder (2) clockwise.
Pipettes m5000 and m10 000:
Carefully place the tip cone cylinder (4) on the piston and screw
clockwise. Make sure that the tip cone (cylinder) is properly
tightened. Avoid over tightening.
2. Attach the tip ejector collar (1) by screwing it clockwise.
3. Place the new tip cone filter on its place.
4. Press the operating button several times to ensure that the
grease has spread evenly.
5. Check the performance of the pipette.
Note: It is always necessary to check the performance of the
pipette after in-house service or maintenance.
11.3. Sterilization of the pipette
The entire mLINE pipette can be
sterilized by steam autoclaving
at 121°C, (252°F), 1 atm for
20 minutes. Remove the tip
cone filter (if fitted, Chapter
8.3). The single channel
pipettes can be autoclaved
without special preparations.
Unscrew the lower part of
the multichannel pipettes by
holding the connecting collar and turning the lower part 360°
counterclockwise (Fig. 10). Put the pipette into the sterilization bag
and place it into the autoclave. After autoclaving the pipette must be
cooled down and left to dry overnight before use. Screw the lower
part of the multichannel pipettes by holding the connecting collar and
turning the lower part 360° clockwise. Make sure that the lower part
is properly tightened. It is recommended to check the performance of
the pipette after every autoclaving. It is also recommended to grease
the piston/seal of the pipette after every 10 autoclaving.
360°
Fig. 10.
Connecting
collar
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12. TESTING THE PERFORMANCE AND RECALIBRATION
It is recommended to check the performance of your Biohit mLINE
pipettes regularly (e.g. every 3 months) and always after in-house
maintenance. However, the user should establish a regular testing
routine for their pipettes with regard to accuracy requirements of
the application, frequency of use, number of operators using the
pipette, nature of the liquid dispensed and the acceptable maxi-
mum permissible errors established by the user. (ISO 8655-1.)
12.1. Testing the performance
Performance testing should take place in a draught-free room at
15 - 30°C, constant to +/- 0.5°C and humidity above 50%. The
pipette, tips and the test water should have stood in the test room
a sufficient time (at least 2 hours) to reach equilibrium with the
room conditions. Use distilled or deionised water (grade 3, ISO
3696). Use an analytical balance with a readability of 0.01 mgs.
(ISO 8655-6.)
Weighing
1. Adjust the desired test volume Vs.
2. Carefully fit the tip onto the tip cone.
3. Fill the tip with test water and expel to waste five times to reach
a humidity equilibrium in the dead air volume.
4. Replace the tip. Pre-wet the tip by filling it once with test water
and expel to waste.
5. Aspirate the test water, immersing the tip only 2-3 mm below
the surface of the water. Keep the pipette vertical.
6. Withdraw the pipette vertically and touch the tip against the
inside wall of the test water container.
7. Pipette the water into the weighing vessel, touching the tip
against the inside wall of the vessel just above the liquid surface
at an angle of 30° to 45°. Withdraw the pipette by drawing the
tip 8-10 mm along the inner wall of the weighing vessel.
8. Read the weight in mgs (mi).
9. Repeat the test cycle until 10 measurements have been
recorded.
10. Convert the recorded masses (mi) to volumes (Vi)
V
i= miZ Z = correction factor (Table 1)
11. Calculate the mean volume (v) delivered:
v= (∑Vi)/10
12. For conformity evaluation calculate the systematic error esof
the measurement:
in μl: es= v- VsVs= selected test volume
or in %: es= 100 (v- Vs)/Vs
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13. For conformity evaluation calculate the random error of the
measurement:
as standard deviation s = ∑(Vi- v)2n = number of
measurement (10)
n - 1
or as coefficient of variation CV = 100s/v
14. Compare the systematic error (inaccuracy) and random error
(imprecision) with the values in the performance specifications
- (p. 49) or the specifications of your own laboratory.
If the results fall within the specifications, the pipette is ready
for use. Otherwise check both systematic and random errors
and, when necessary, proceed to the recalibration procedure
(Chapter 12.2).
Note: Systematic error (inaccuracy) is the difference between the
dispensed volume and the selected test volume. Random error
(imprecision) is the scatter of the dispensed volumes around the
mean of the dispensed volume. (ISO 8655-1.)
Note: Biohit specifications are achieved in strictly controlled condi-
tions (ISO 8655-6). The user should establish own specifications
based on the field of use and the accuracy requirements placed on
the pipettor (ISO8655-1).
Table 1
Note: This method is based on ISO 8655.
Z-values (μl/mg):
Temp. (°C) Air Pressure (kPa)
95 100 101.3 105
20.0 1.0028 1.0028 1.0029 1.0029
20.5 1.0029 1.0029 1.0030 1.0030
21.0 1.0030 1.0031 1.0031 1.0031
21.5 1.0031 1.0032 1.0032 1.0032
22.0 1.0032 1.0033 1.0033 1.0033
22.5 1.0033 1.0034 1.0034 1.0034
23.0 1.0034 1.0035 1.0035 1.0036
23.5 1.0036 1.0036 1.0036 1.0037
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12.2. Recalibration
The calibration of your mLINE pipette has been factory checked
by using forward pipetting technique and certified at 22°C using
grade 3 distilled water according to ISO 3696. The calibration is
based on ISO 8655-6: Gravimetric test method for volumetric
instruments. The pipette's specifications are guaranteed only with
genuine Biohit tips. If you find the pipette to be inaccurate after
performance testing, please follow the instructions below:
1. Remove the lid of the calibration nut, located at the backside
of the handle, with the aid of the calibration tool (Fig. 11).
2. Place the hexagonal head of the calibration tool into the hole
of the calibration nut.
3. Turn the adjustment lock counterclockwise to decrease and
clockwise to increase the volume.
4. Repeat testing the performance procedure (Chapter 12.1.).
Continue until the results are correct.
Note: Recalibration is recommended if reverse pipetting technique
is used.
Note: Biohit offers accredited calibration service. Please contact
your local Biohit representative for further information.
Fig. 11.
–
+
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Symptom Possible cause Solution
Droplets left inside Unsuitable tip Use original tips
the tip
Leakage or pipetted Non-uniform wetting Attach new tip
volume too small of the plastic
Tip incorrectly Attach firmly
attached
Unsuitable tip Use original tips
Foreign particles Clean the tip cone,
between tip and attach new tip
tip cone
Tip cone holder Tighten the tip
incorrectly tightened cone holder
Pipette damaged Return to your
Biohit service
representative
for servicing
Pipette out of Incorrect operation Follow instructions
established
specifications Unsuitable tip Use original tips
Calibration altered Recalibrate
Operating button Liquid has penetrated Clean and grease
or moves tip cone and dried jammed the piston
erratically /seal and the tip
cone
Safe Cone filter has Change filter
been contaminated
Insufficient amount of Grease
grease on a piston accordingly
and seal
Tip ejector jammed Tip ejector collar has Remove and clean
or moves erratically been contaminated the ejector collar
and tip cone
13. TROUBLE SHOOTING
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