Biohit eLINE Lite User manual

Instruction Manual
eLINE®
Dispenser
Dispenser
Lite
Lite

1
CONTENTS
1. INTENDED USE ......................................................................... 2
2. PRODUCT DESCRIPTION........................................................ 2
3. QUICK START............................................................................ 3
3.1. Unpacking......................................................................... 3
3.2. Charging the Dispenser before first use.......................... 4
3.3. Operating Instructions...................................................... 5
3.3.1. Display .............................................................................. 6
3.3.2. Operating Modes:............................................................. 6
3.3.3. Keyboard and operation buttons ..................................... 6
3.3.4. Reset Button..................................................................... 7
3.3.5. Start Button ...................................................................... 7
3.3.6. Tip Attachment and Ejection ........................................... 7
4. CHARGING OF THE DISPENSER............................................ 8
4.1. Charging with the Biohit eLINE Charging Stand and
Charging Carousel............................................................ 9
4.2. Charging through direct charging system ..................... 10
4.3. Charging Recommentations .......................................... 10
4.4. Electrical specifications ................................................. 11
5. PROGRAMMING PRINCIPLES .............................................. 11
5.1. Programming Map.......................................................... 12
5.2. Programming of the multiple dispensing mode
using the TipGuide ......................................................... 13
6. MAINTENANCE ....................................................................... 15
6.1. Cleaning the dispenser................................................... 15
6.2. Battery replacement....................................................... 16
7. TROUBLESHOOTING ............................................................. 16
8. WARRANTY INFORMATION .................................................. 18
9. PERFORMANCE SPECIFICATIONS ...................................... 19
10. RECYCLING INSTRUCTION (WEEE) ....................................20

2
1. INTENDED USE
This liquid handling device is designed and manufactured to be used with
the IVD-product as an IVD-accessory and as a stand alone laboratory
equipment.
2. PRODUCT DESCRIPTION
The Biohit eLINE Lite is an electronic dispenser for the multiple dispensing
of the liquids. The dispenser offers you the latest technology and innovations
in the field of the hand held liquid handling devices. The attractive and
ergonomic design of the eLINE Lite together with an unique electronic tip
ejection operation take the effort out of multiple dispensing and reduce
the risk of repetitive strain injuries (RSI) that are common in mechanical
pipetting.
The microprocessor controlled system and the superior construction of the
Biohit eLINE Lite Dispenser enable maximum liquid handling performance
with high levels of accuracy and precision.
The eLINE Lite Dispenser uses positive displacement principle, and is
thereby an ideal tool for
• Long pipetting series
• Viscous, infectuous and high vapour pressure liquids
• Radioactive and toxic liquids
Biohit eLINE Lite Dispenser
Cat. No. 73080X
X: 0=Without adaptor; 1= With universal AC-adaptor (Euro, USA/Jpn, UK
and China plugs)
Biohit Dispenser Tips
Cat.
No.
Tip
volume
(ml)
Type Volume
min/max(μl)
Incre-
ment
(μl)
Number of
dispensings
min/max
Qty/
Unit
792017
792026
0.1 Non Sterile
Pre-sterilised
1/100 0.2 1/100 100
792018
792027
0.2 Non Sterile
Pre-sterilised
2/200 0.4 1/100 100
792019
792028
0.5 Non Sterile
Pre-sterilised
5/500 1 1/100 100
792020
792029
1.0 Non Sterile
Pre-sterilised
10/1000 2 1/100 100
792021
792030
2.5 Non Sterile
Pre-sterilised
25/2500 5 1/100 100

3
Cat.
No.
Tip
volume
(ml)
Type Volume
min/max(μl)
Incre-
ment
(μl)
Number of
dispensings
min/max
Qty/
Unit
792022
792031
5.0 Non Sterile
Pre-sterilised
50/5000 10 1/100 100
792023
792032
10.0 Non Sterile
Pre-sterilised
100/10 000 20 1/100 100
792024
792033
25.0 Non Sterile*
Pre-sterilised*
500/25 000 50 1/50 25
792025
792034
50.0 Non Sterile*
Pre-sterilised*
1000/50 000 100 1/50 25
792038 Dispenser Tips Starter Kit includes:
• 0.5 ml/20 pcs
• 1,0 ml/20 pcs
• 2.5 ml/20 pcs
• 5.0 ml/20 pcs
• 10.0 ml/20 pcs
* 1 adapter included
Note: The tips are disposable, do not reuse them!
3. QUICK START
3.1. Unpacking
The Biohit eLINE Lite Dispenser package contains:
- eLINE Lite Dispenser
- Tilting piece
- Instruction manual
- Performance certificate in accordance with ISO 8655-5
- Demo package of the Tips
• 0.1 ml, 1 pc
• 0.2 ml, 1 pc
• 0.5 ml, 5 pcs
• 1.0 ml, 5 pcs
• 2.5 ml, 5 pcs
• 10.0 ml, 5 pcs
• 25.0 ml, 1 pc
• Adapter for 25 ml tip, 1 pc
• AC-adaptor (if ordered)
Please check that all items are included and no damage has occurred during
shipment.

4
3.2. Charging the Dispenser before first use
The battery is protected against discharging during storage with a plastic
protector. Before use remove the plastic protector (Fig. 1.)
1. Push the charging
buttons simultaneuously
in order to open
the battery cover
2. Remove the plastic
protector
Note: It is recommended to charge the pipette for 12 hours before initial use.
The pipette may only be charged with original Biohit eLINE Charging Stand,
Charging Carousel or Biohit eLINE AC-adaptor (See Section 3.)
1. 1.
2.
Fig. 1.

5
3.3. Operating Instructions
The control and programming of the Biohit eLINE Lite Dispenser is performed
using the display, keyboard, operating buttons and tip. (Fig. 2 and 3.)
Keyboard
Display
Start
button
Tip
ejection
buttons
Tip
connector
Biohit
Dispenser
Tip
Charging
contacts
Battery
cover
Reset
button
Fig. 2.
Fig. 3.

6
COUNT
100 x
SPEED
IN
+ –
TIP SIZE
4.00 µl
55.0ml
3.3.1. Display
Note: When the dispenser is not connected to the charging unit the dispenser/
display will automatically hibernate after 10 minutes. The dispenser can be
switched on by pressing any key.
3.3.2. Operating Modes
The eLINE Lite Dispenser features multiple dispensing
mode with Tip Guide. The Tip Guide is a unique built-
in guide that selects the optimal tip size according to
selected volume and number of dispensings.
3.3.3. Keyboard and operation buttons
Aspirating/
Dispensing
direction
Battery
symbol
Speed
in/out
Aliquots/
counter Volume
Selected
tip
SPEED
IN
TIP SIZE
2.36 ml
3
3x
10ml
Changes volume,
aliquots and speed
Activates the
speed function
Confirms the
settings
Ejects the tip
START:
Triggers the
pipetting operations
and confirms the
selected tip

7
3.3.4. Reset Button
To reset the dispenser, press the reset button on the battery cover. The
display shows Eand TIP in blinking form and pressing the tip ejector button
will reset the pipette to the d-mode.
Note: The dispenser should never be reset
during pipetting! Resetting will cause the
plunger to move to the home position and it
will empty the contents of the tip. The tip is
also ejected in the end.
3.3.5. Start Button
The start button triggers the aspiration and
dispensing operations according to the
selected operating mode. Only a soft touch
is required to operate the button.
3.3.6. Tip Attachment and
Ejection
Tip attachment
1. Push the tip into the tip connector. Make
sure that the tip is properly attached.
(Fig. 4.)
2. Confirm the tip size by double clicking the
Start-button according the instruction on
the page 15.
Releasing the tip:
1. Press the Tip Ejection button. The tip is
released immediately (Fig. 5.)
Note: After the dispensing cycle has been
completed a small residual amount of
fluid may have been left in the tip. During
the tip ejection it is possible that a part
of the remaining fluid comes out of the
tip. Therefore it is recommended that the
tip ejection is performed above a waste
container.
1
1
2
Fig. 4.
Fig. 5.

8
4. CHARGING OF THE DISPENSER
The Biohit eLINE Lite Dispenser should be charged using the original Biohit
eLINE Charging Stand, Charging Carousel or AC-adaptor.
When the dispenser is properly connected to the charger, the led indicator
(Fig. 6.) lights up and stays on as long as the dispenser is connected to the
charger.
When the dispenser requires charging, the battery symbol and “lo bat”-
text will appear in the display (Fig. 6.). You need to confirm that by pressing
“enter”, only after that you can complete your work, and/or start charging the
dispenser. Please note that if “enter” is not pressed the dispenser will not
start charging, even placed in charging stand/connected to AC-adaptor.
Note: In case you continue to work with the dispenser after the low bat-
alarm, the battery will eventually be completely discharged. Then the
battery-symbol and led indicator start to blink, and there is no response
from operating buttons. Charge the dispenser.
Initial charging of 30 minutes is required before use, if the battery is totally
discharged. During that time the “Load”-text is displayed and you can’t use
the dispenser.
When the text disappears from the display, you can continue to work with the
dispenser keeping it still connected to the charger.
Fig. 6.
Led indicator
Battery symbol

9
4.1. Charging with the Biohit
eLINE Charging Stand and
Charging Carousel
The Biohit eLINE Charging Stand and
Carousel are desktop charging units.
The Charging Stand accommodates one
Biohit eLINE and Charging Carousel four
Biohit eLINE Dispensers.
The Charging Stands and Carousels
operate through the metal contacts
placed in the sides of the Dispenser and
the head of the Charging unit. (Fig. 7.)
Before operating the AC-adaptor must be
connected to the socket at the rear of the
Charging Stand (Fig. 7.) as well as to the
electrical outlet. Before connecting the
AC-adaptor to the electrical outlet, check
that its voltage setting corresponds to the
voltage setting of your electrical outlet.
Note: The Charging Carousel is used to
charge the dispenser without the tip. If
you need to charge the dispenser with the
tip attached, you can use the Charging
Stand with the Tilting Piece mounted (Fig.
8.).
Cat. No. Product
730981 Biohit eLINE Charging Stand
730991 Biohit eLINE Charging Carousel
With universal AC-adaptor (Euro, USA/Jpn, UK and China plugs)
1. Check that the plastic protector is removed and the battery cover is
properly closed
2. Place the dispenser into the Charging unit. Make sure that the metal
contacts at the sides of the dispenser are properly fitted to the tracks of
the metal contacts in the Charging head.
Fig. 8.
Fig. 7.

10
3. When charging, the battery-symbol and the led indicator will be displayed.
The charging time for completely discharged batteries is 6 hours (initial
charge 12 hours).
4. When the battery is fully charged the battery-symbol disappears from the
display. Otherwise the display will stay on and other information on the
display will be visible.
4.2. Charging through direct charging system
The AC-adaptor supplied with the Biohit eLINE Lite
Dispenser or Charging Stand can be used for direct
charging of the Biohit eLINE Lite Dispenser. The
socket for the AC-adaptor is placed at the back of the
dispenser (Fig. 9.)
Cat. No. Product
731108 Biohit eLINE universal AC-adaptor,
(Euro, USA/Jpn, UK and China plugs)
1.
Before connecting the AC-adaptor to the electrical outlet, check that its
voltage settings corresponds to the voltage settings of the electrical outlet.
2. Check that the battery protector is removed and the battery cover is
properly closed.
3. Connect the AC-adaptor to the electrical outlet as well as to the socket at
the back of the dispenser
4. The charging time is 6 hours for the completely discharged battery.
5. When the battery is fully charged the battery-symbol disappears from the
display. Otherwise the display will stay on and other information on the
display will be visible.
Note: It is possible to continue pipetting while the eLINE Lite Dispenser is
charged through the AC-adaptor. With fully discharged battery 30 minute
initial charging is recommended before continuing the pipetting.
4.3. Charging Recommentations
When the eLINE Lite Dispenser is not in use it is recommended to keep it
always in the charging unit. This ensures that the battery is kept fully charged
and the pipette is always ready for use.
However, during long storage periods (holidays etc.) it is recommended to
disconnect the Charging Stand and Carousel from the mains outlet. It is also
advisable to remove the battery pack from the dispenser. This protects the
batteries against discharging during the long storage period.
Fig. 9.

11
Note: The battery won’t get damaged when the dispenser is kept on
the charging unit: the charging process is intelligently controlled by a
microprocessor.
Note: If battery is kept as full as possible, it will lengthen its lifetime.
4.4. Electrical specifications
Battery
• Rechargeable NiMH battery back with protection circuit
• 4.8 V, 500 mAH
• Charging time 6 hours for empty batteries
• Initial Charging 12 hours
AC-adaptor for Charging Stand or direct charging
• Input voltages and main plug according to local requirements
• Output voltage 9 VDC, 360 mA
AC-adaptor for Charging Carousel
• Input voltages and main plug according to local requirements
• Output voltage 9 VDC, 1200 mA
5. PROGRAMMING PRINCIPLES
Multiple dispensing
The Biohit eLINE Lite Dispenser features multiple dispensing mode with
unique built in Tip Guide that selects the optimal tip size for you.
In this mode the Dispenser performs repetitive dispensing of a selected
volume. The sum of the dispensing aliquots and an automatically selected
excess volume is aspirated into the tip. The excess volume is needed to
ensure equal operating conditions for each dispensing step.

12
5.1. Programming Map
Multiple dispensing using Tip Guide
Adjust the dispenser to the initial position
by pressing the Tip Ejection-button
Select the aliquot volume
Confirm the selection
Confirm the selection
Confirm the selection
Select the number of aliquots
Select the tip size
Tip Guide: Shows the best tip option. If there are also other
tip options available the TIP SIZE-text is blinking. View the
other options by pressing the Arrow up/down key.
Do you want to change the speed?
Note! Dispenser is now fixed to the selected tip!
Start pipetting!
YES NO
Attach the tip. Note! The TIP text
is blinking on the display.
Confirm the programming by
double clicking the START
button
Select the
speed in
Select the
speed out

13
Tip confirmation after tip ejection
Tip ejection If you want to
change the tip
size
if you want to
continue with the
same tip size
2 x
Change
- the aliquot volume
- the number of aliquots
- the tip size
2 x
5.2. Programming of the multiple dispensing
mode using the TipGuide
1. Adjust the Dispenser to the initial position
by pressing .
Note: The tip will be ejected if it is attached!
2. Select the desired aliquot volume by pressing
Note: When holding down the the volume display start to scroll
faster
3. Press to confirm the selection
4. Select the number of aliquots by pressing
5. Press to confirm the selection
6. Now the TipGuide shows the best tip option.
If there are also other tip options available,
the Tip-size text is blinking. View the other
options by pressing
7. Co n firm your selection by pressing and continue from step 12.
Or press to change the aspiration/dispensing speeds.
TIP SIZE
tIP ml
10 ml
count
2 x
4.00 ml
TIP SIZE
10 ml

14
8. Select the speed in with (1=Min and 5=Max)
9. Press or to confirm selection and to display the speed out
10. Select the speed out with (1=Min and 5=Max)
11. Press to confirm selection
12. Now the text “TIP” is blinking on the display. Attach the tip
13. Confirm the programming by double clicking the START-button
Note: Dispenser is now fixed to the selected tip volume range
14. Place tip into the liquid and press
START-button to aspirate
15. Discard the prime excess by
pressing the START-button
16. To dispense, position the tip above the
receiving vessel and press START-button
and repeat until the cycle is completed
17. Finally position the tip to discard
excess volume and click twice
the START-button
18. Now the Dispenser is ready for next
pipetting cycle
Note: It is also possible to continue to aspirate and dispense the same
volume without the empty function. To continue, keep the START-button
pressed during the last dispensing and within one second the direction of
the arrow will change. Keeping the START button depressed, place the tip
into the liquid again and then release the START button to aspirate the next
sample.
OUT
E
SPEED
IN
TIP SIZE
4.00 ml
3
10 ml
2 x
SPEED
OUT
TIP SIZE
4.00 ml
3
10 ml
2 x
SPEED
IN
TIP SIZE
4.00 ml
3
10 ml
2 x

15
If you continue with the same tip
a. Change the volume by pressing
b. Confirm your selection by pressing
c. Change the number of aliquots by pressing
d. Confirm your selection by pressing
If you want to change the tip size or tip, go to step 1.
If you want to eject the tip, press
Note: It is possible to interrupt the dispensing by pressing the ENTER
button . The display shows E and the tip can be emptied by pressing the
START button .
Note: When aspirating the liquid using the 25 or 50 ml tip, let the tip stay in
the liquid a few seconds after the plunger has stopped moving. This way you
will ensure that all liquid has been aspirated into the tip.
Note: It is possible to work with aqueous liquids and liquids with higher
viscosity. However, it is recommended always to choose the tip with the
smallest applicable volume in conjunction with high viscosity liquids. Please
note, the specifications are given in accordance with ISO8655-5.
6. MAINTENANCE
6.1. Cleaning the dispenser
To ensure trouble-free operation it is recommended to apply regular
maintenance and cleaning to Biohit eLINE Lite Dispenser.
To clean and decontaminate the outer surface use Proline Biocontrol (Cat.
No. 724004) and a soft lint-free cloth. Gently clean the surface of the pipette
with the moistened cloth and wipe dry. It is possible to use ethanol (70%)
isopropanol (60%) or mild detergent as a cleaning agent.
Note: Remove the battery pack from the dispenser prior the cleaning!
It is recommended always to use gloves when cleaning the dispenser. Do not
let the liquid enter the pipette handle!

16
6.2. Battery replacement
The design of the Biohit eLINE dispenser enables fast and easy battery
replacement. The battery replacement pack includes 4 NiMH batteries fixed
to the battery cover. If the batteries do not hold a sufficient charge for proper
operation follow these steps for replacement of the battery pack.
1. Hold the dispenser in a horizontal position
with the display facing up.
2.
Press the two charging contacts simultaneously
to release the battery pack.
3. Place the new battery pack into the
compartment and close the battery cover
by pressing it lightly.
Note: The battery cover pack fits in to the
compartment one way only. If the pack is
inserted incorrectly the battery cover will not
close properly.
Note: Only use battery packs supplied by the manufacturer. The use of any
other type of battery will immediately void the warranty!
7. TROUBLESHOOTING
The Biohit eLINE Lite Dispenser have a onboard monitoring program to control
the performance of each pipetting action. If any error message appears to
the display it means that the Dispenser has been unable to perform the
attempted action properly. In case of an error the display will show ER and
PRSS ENT. message. To clear the message please follow these steps:
1.
Clear the error message by pressing . The display will show E and .
2. Press the and the blunger moves to the home-position. The display
will show E and TIP.
3. Remove the tip by pressing the tip ejection button
Note: Repeated occurrence of error messages indicates an internal error
causing failure of the Dispenser to complete the execution of the pipetting.
The Dispenser therefore needs to be returned to the nearest Biohit Service
Centre or your local Biohit service representative for repair.
Fig. 10.

17
Symptom Possible cause Solution
Display is blank and
there is no response
when pressing the
buttons
Battery is fully
discharged
Recharge the battery
Leakage or pipetted
volume too small
Tip incorrectly
attached
Unsuitable tip
Foreign particles
between the tip and
tip connector
Attached new tip
Attach firmly
Use original tips
Clean the tip
connector and attach
new tip
Dispenser out of
the established
specifications
Unsuitable tip
Instrument damaged
Use original tips
Return to your Biohit
service representative
for servicing
Tip ejector jammed or
moves erratically
Tip connector has
been contaminated
Tip connector
mechanism damaged
Clean the Tip
connector
Return to your Biohit
service representative
for servicing
Continuous error
message
Instrument damaged Return to your Biohit
service representative
for servicing

18
8. WARRANTY INFORMATION
Biohit eLINE Lite Dipenser is covered by a warranty for two years against
defects in material and workmanship (excluding the battery pack). Should
your dispenser fail to function according to specifications at any time, please
contact your local Biohit representative immediately.
ANY WARRANTY WILL, HOWEVER, BE DEEMED AS VOID IF FAULT
IS FOUND TO HAVE BEEN CAUSED BY MALTREATMENT, MISUSE,
UNAUTHORIZED MAINTENANCE A OF SERVICE OR NEGLIGENCE
OF REGULAR MAINTENANCE AND SERVICE, ACCIDENTAL DAMAGE,
INCORRECT STORAGE OS USE OF THE PRODUCTS FOR OPERATIONS
OUTSIDE THEIR SPECIFICATIONS, CONTRARY TO THE INSTRUCTIONS
GIVEN IN THIS MANUAL OR WITH OTHERTHAN THE MANUFACTURER’S
ORIGINAL TIPS.
Each Biohit eLINE Lite Dispenser is tested before shipping by the
manufacturer. The Biohit Quality Assurance Procedure guarantees that the
Biohit eLINE Lite Dispenser you have purchased is ready for use.
Each Biohit eLINE Lite Dispenser is CE-marked, fulfilling the requirements
of the EN 55014, 1993 / EN 55104, 1995 / ISO 13485:2003 and IVD Directive
(98/79/EC).

19
9. PERFORMANCE SPECIFICATIONS
Note: The manufacturer’s specifications should be used as guidelines when
establishing your own performance specifications accordance with ISO
8655-5.
eLINE Lite Dispenser and Biohit Dispenser Tip system
Tip
volume
(ml)
Volume
min/
max (μl)
Increment
(μl)
Test
volume
(μl)
Inacc.
(%)
Impr.
(%)
Number
of
dispensing
min/max
0.1 1/100 0.2 100
10
1.00
1.00
0.50
2.00
1/100
0.2 2/200 0.4 200
20
1.00
1.70
0.50
2.50
1/100
0.5 5/500 1 500
50
0.80
1.00
0.60
3.00
1/100
1.0 10/1000 2 1000
100
0.50
0.50
0.20
0.60
1/100
2.5 25/2500 5 2500
250
0.60
0.60
0.20
0.40
1/100
5.0 50/5000 10 5000
500
0.60
0.60
0.15
0.50
1/100
10.0 100/10 000 20 10 000
1000
0.40
0.40
0.20
1.00
1/100
25.0 500/25 000 50 25 000
2500
0.50
0.50
0.15
0.50
1/50
50.0 1000/50 000 100 50 000
5000
0.30
0.30
0.15
0.70
1/50
Note: The specifications are valid only to the eLINE Lite Dispenser and Biohit
eLINE Dispenser Tip combination.