Biosan FTA-1 User manual


Edition 1.-4.03 3
Contents
1. About this edition of user instructions.......................................................................3
2. Safety precautions ...................................................................................................4
3. General information..................................................................................................6
4. Getting started..........................................................................................................7
5. Operation ...............................................................................................................10
6. Specifications.........................................................................................................11
7. Ordering information ..............................................................................................12
8. Chemical compatibility............................................................................................13
9. Care and maintenance...........................................................................................16
10. Storage and transportation.....................................................................................17
11. Warranty.................................................................................................................18
12. EU Declaration of conformity..................................................................................19
1. About this edition of user instructions
1.1 The current edition of the user instructions applies to the following models:
Model and name
Version
FTA-1, aspirator with trap flask
V.4AW
FTA-2i, aspirator with trap flask
V.1A01
1.2 Edition 1.-4.03 –July of 2022

4 Edition 1.-4.03
2. Safety precautions
Caution!
Make sure you have fully read and understood the present Manual before
using the equipment. Please pay special attention to sections marked by
this symbol.
2.1 Icons used on the unit and packaging
CE marking, manufacturer affirms conformity with European health, safety, and
environmental protection standards, see 12.1
WEEE directive marking, see 12.1
Polarity of the power connector
Equipment uses direct current
Pump exhaust on FTA-2i, connect to the means of air filtration, see 8.1
2.2 General safety
•The protection provided can be ineffective if the operation of the appliance does not
comply with the manufacturer's requirements.
•Save the unit from shocks and falling.
•Store and transport the unit as described in section 10. Storage and transportation
on page 17.
•Use only original parts and accessories, provided by manufacturer for this product.
•Before using any cleaning or decontamination methods except those recommended
by the manufacturer, check with the manufacturer that the proposed method will not
damage the equipment.
•Do not make modifications in design of the unit.
2.3 Electrical safety
•Connect only to the mains with voltage listed on the serial number label.
•Use only the external power supply provided with this product.
•Ensure that the power plug is easily accessible during use.
•Disconnect the unit from the mains before moving.
•If liquid penetrates into the unit, disconnect it from the mains and have it checked by
a repair and maintenance technician.
•Do not operate the unit in premises where condensation can form. Operating condi-
tions of the unit are defined in section 6. Specifications on page 11.

Edition 1.-4.03 5
2.4 During operation
•Do not operate the unit in environments with aggressive or explosive chemical mix-
tures. Please contact manufacturer for possible operation of the unit in specific at-
mospheres.
•Do not operate the unit if it is faulty or has been installed incorrectly.
•Do not use outside laboratory rooms.
•Do not use the unit for aggressive organic compounds, chlorinated solvents and
strong conjugated acids, see section 8. Chemical compatibility on page 14.
2.5 Biological safety
•The user is responsible to carry out appropriate decontamination if hazardous mate-
rial spills on or penetrates into the equipment.
•Empty the trapping flask when the liquid level reaches maximum. Take necessary
precautions utilizing waste liquid in accordance with general laboratory standards.

6 Edition 1.-4.03
3. General information
FTA-1 & FTA-2i aspirators with trap flask have been designed for liquid removal from
micro-test tubes (e.g., for supernatant removal during DNA/RNA extraction), ELISA plates
(e.g., performing several repeated well washing cycles), cell culture flasks (cell media ex-
change and washing during cell growth) and other vessels during various laboratory tech-
niques.
Model FTA-1 has a 1 L glass trap flask and is designed to be a low-cost solution.
Aspiration is possible with a single standard 200 μL tip and the 8-tipped MA-8 adapter.
Model FTA-2i comes with a 2 L polypropylene trap flask and a liquid overflow sensor
that stops collected material from damaging the filter. Aspiration is possible with single 200
and 1000 μL tip using the MA-U adapter or with the handheld vacuum controller HAS-1 with
different attachments such as 8-channel adapter with stainless steel tips, 8-channel adapter
for 200 μL tips, single channel adapter for 200 μL tips and others.
Microbiologic suction filter eliminates risks of contamination caused by bacteria, vi-
ruses, and infected particles from samples. Such contamination can be distributed by suction
pump or central vacuum units. Microbiological suction filter is hydrophobic and has very high
contamination removal bacterial efficiency –up to 99.99% of particles bigger than 0.027
micrometres (e.g., Hepatitis A, B and C, HIV, etc.).
In order to prolong performance and efficiency of device it is not allowed to use ag-
gressive organic compounds (e.g., phenol) and strong conjugated acids (e.g., guanidine) for
aspiration. Please, contact manufacturer in case of any questions regarding operation of
device.

Edition 1.-4.03 7
4. Getting started
4.1 Unpacking. Remove packing materials carefully and retain them for future shipment
or storage of the unit. Examine the unit carefully for any damage incurred during
transit. The warranty does notcover in-transit damage. Warrantycovers only the units
transported in the original package.
4.2 Complete set. Package contents:
4.2.1 FTA-1:
- FTA-1, aspirator with trap flask ........................................................................1 pce.
- Hydrophobic filter set .........................................................................................1 set
- Aspiration tips, 200 µL....................................................................................50 pcs.
- Holder tubes (1.5 ml) .....................................................................................10 pcs.
- Spare hydrophobic filter ...................................................................................1 pce.
- External power supply .....................................................................................1 pce.
- Operating instructions, certificate ...................................................................1 copy
- MA-8, 8-channel aspiration tip with holder................................................on request
4.2.2 FTA-2i:
- FTA-2i, aspirator with flask trap........................................................................1 pce.
- MA-U, universal adapter ..................................................................................1 pce.
- Hydrophobic filter set .........................................................................................1 set
- Aspiration tips, 200 µL....................................................................................50 pcs.
- Aspiration tips, 1000 µL..................................................................................10 pcs.
- Holder tubes (2 ml) ..........................................................................................5 pcs.
- Spare hydrophobic filter ...................................................................................1 pce.
- External power supply .....................................................................................1 pce.
- Operating instructions, certificate ...................................................................1 copy
- Extended tubing (2 m), with fittings and MA-U adapter.............................on request
- MA-8, 8-channel aspiration tip with holder................................................on request
- HAS-1, hand operator set with SH-6 stand...............................................on request
Figure 1. Assembled FTA-1 Figure 2. Installation of MA-8 aspiration tip

8 Edition 1.-4.03
Figure 3. FTA-2i front view Figure 4. FTA-2i lid with sensor and tubing
Figure 5. MA-U, universal adapter
Figure 6. FTA-2i filter setup Figure 7. FTA-2i rear panel
4.3 FTA-1 setup.
- Place the unit on horizontal even working surface.
- Install the trapping flask (fig. 1/5) on the unit.
- Assemble the lid, aspiration tubing and filter set (fig. 1/2). Attach aspiration tip (fig.
1/6) to the tubing.
Caution!
Position the filter with the IN marking pointing towards the unit (fig. 1/2).
- Screw on the lid with the filter set and aspiration tubing to trap flask. Ensure that the
filter set is tightly inserted.
- Connect the filter tubing to the pump (fig. 1/1) located at the top of the base behind
the trap flask, by turning the fitting clockwise.
- Place the microtubes for aspiration tip into the holder (fig. 1/4).
- Connect the external power supply unit into the socket at the rear side of the unit
and position the unit for an easy access to the external power supply and the power
switch.

Edition 1.-4.03 9
4.3.1 Optional MA-8 aspiration tip installation. Remove microtubes and aspiration tip
from the holder (fig. 1/4). Place MA-8 storing holder (fig. 2/1) into the holder. Remove
aspiration tip (fig. 1/6) from the aspiration tubing and connect the MA-8 aspiration tip
(fig. 2/2). Store the aspiration tip in the new holder.
4.4 FTA-2i setup.
- Place the unit on horizontal even working surface.
- Install the trapping flask (fig. 3/2) on the unit.
- Screw on the lid with the sensor needles and valves for tubing (fig. 3/1) to trapping
flask.
- Assemble the filter set (figure 6) and connect it to the port on the rear panel of the
unit (fig. 7/1).
Caution!
Position the filter with the IN marking pointing away from the unit (fig. 6/1).
- Attach the fitting (fig. 4/1) of the tubing to the filter (fig. 4/2) to the valve with white
ring on the lid.
- Ensure that the filter set is tightly fixed in the slot (fig. 7/1).
- Assemble the MA-U aspiration tip set (figure 5).
- Attach the fitting (fig. 4/3) of the tubing for liquids (fig. 4/4 and 5/3) to the valve with
the blue ring on the lid.
- Connect the sensor cable (fig. 4/5) to the lid of the unit and to the port on the rear
panel of the unit (fig. 7/4).
- Place the microtubes for aspiration tip into the holder (fig. 3/3).
- Connect the external power supply unit into the socket at the rear side of the unit
(fig. 7/5) and position the unit for an easy access to the external power supply and
the power switch.
4.4.1 Optional MA-8 aspiration tip installation. Remove microtubes and aspiration tip
from the holder (fig. 3/3). Remove the aspiration tip (fig. 5/1) from the MA-U adapter
and connect the MA-8 aspiration tip (fig. 8/1A).
4.4.2 Optional HAS-1 hand operator installation. Unscrew the MA-U adapter from the
aspiration tube (fig. 5/3) and connect the HAS-1 hand operator (fig. 8/5) by screwing
it to the fitting.

10 Edition 1.-4.03
5. Operation
5.1 Connect the external power supply to the mains.
5.2 Turn the Power switch (fig. 1/3 or 7/2) to the position I(on). The pump starts working.
5.3 (For model FTA-2i) Regulate the aspiration intensity with the regulator knob on the
front panel of the unit (fig. 3/4). Clockwise turning direction increases power, counter
clockwise –decreases. Current intensity is indicated by a light ring around the regu-
lator knob.
5.4 Touch the surface of the liquid with the aspiration tip (fig. 1/6, 5/1 or MA-8) to remove
the liquid.
Caution!
During operation, do not allow the collectedliquid to exceed the maximum
level. Empty the trapping flask when the liquid level reaches maximum
allowed level, according to 5.6.
Note.
In model FTA-2i, when collected liquid exceeds the maximum level, the
sensor turns off the pump and produces sound and light warning sig-
nals.
5.4.1 (For model FTA-2i) When using the optional HAS-1 hand regulator, touch the liquid
with the aspiration tip, then press and hold the blue button on the regulator to collect.
5.5 After finishing the operation, switch off the Power switch and disconnect the external
power supply from the mains.
5.6 Emptying the trapping flask. Turn off the unit. Remove the tubings and cables from
the lid. Remove the closed flask from its place. Unscrew the lid and empty the trap-
ping flask. Screw the lid back on and return the trapping flask on the unit. Replace
the tubings and the cables.
Caution!
Take necessary precautions in utilizing waste liquid in accordance with
general laboratory standards. For information regarding autoclaving pro-
cedures, see 9.2.4.

Edition 1.-4.03 11
6. Specifications
The unit is designed for operation in cold rooms, incubators (except CO2incubators)
and closed laboratory rooms at ambient temperature from +4°C to +40°C in a non-condens-
ing atmosphere and maximum relative humidity 80% for temperatures up to 31°C decreasing
linearly to 50% relative humidity at 40°C. Operating altitude above sea level is up to 2000 m.
Biosan is committed to a continuous programme of improvement and reserves the
right to alter design and specifications of the equipment without additional notice.
Model
FTA-1
FTA-2i
Aspiration speed
up to 1.7 mL/s
up to 27.8 mL/s
Vacuum regulation
–
Yes
Working vacuum pressure (at sea level)
-500 mbar
-200 … -800 mbar
Overflow protection sensor
–
Yes, invasive
Overflow protection actions
–
Pump stop, sound and light signals
Trapping flask
1 L, glass
2 L, polypropylene (PP)
Air filtration
Hydrophobic microbiologic air filter 2200/021
Filter pore diameter
0.027 μm
Operating voltage
12 V=
Operating current
300 mA
1 A
Power consumption
3.6 W
10.8 W
External power supply
input 100–240 V~, 50/60 Hz; output 12 V=
Dimensions
160x210x340 mm
185x290x390 mm
Weight, accurate within ±10%
1.7 kg
1.85 kg
1
Eliminates risk of contamination from the trapping flask with bacteria, viruses, and infected particles

12 Edition 1.-4.03
7. Ordering information
7.1 Models and versions available:
Model
Version
Catalogue number
FTA-1, aspirator with trap flask
V.4AW
BS-040108-AAG
FTA-2i, aspirator with trap flask
V.1A01
BS-040120-A02
7.2 To inquire about or order the optional accessories or the replacement parts, contact
Biosan or your local Biosan representative.
7.2.1 Optional accessories:
Description
Catalogue number
МА-8, 8-channel aspiration tip (fig. 8/1A) with holder (fig. 8/1B)
BS-040108-BK
HAS-1, hand operator set (only for FTA-2i). Set includes:
Handheld vacuum controller with adapter (fig. 8/5),
8-channel adapter (with ejector) for 200 µL tips (fig. 8/6),
1-channel adapter with 125 mm stainless steel pin (fig. 8/7),
1-channel adapter with 40 mm stainless steel pin (fig. 8/8),
8-channel adapter with 35 mm stainless steel pin (fig. 8/9),
1-channel adapter (with ejector) for 200 µL tips (fig. 8/10C),
Stand SH-6 for hand operator parts (fig. 8/10)
BS-040118-PK
Extended tubing (2 m), with fittings and MA-U adapter
BS-040120-DK
7.2.2 Replacement parts for FTA-1.
Description
Catalogue number
Hydrophobic filter
BS-040108-S25
Aspiration tip, 200 µL, 1000 pcs. (fig. 8/2)
BS-000001-S04
Aspiration tip, 8 channels, for MA-8 (fig. 8/1A)
BS-040108-S24
Trapping flask, 1 L, glass, with lid, inserts and sealing ring
BS-040108-S11
Tubing and fitting set
BS-040108-S22
7.2.3 Replacement parts for FTA-2i.
Description
Catalogue number
MA-U, universal adapter for 200/1000 µL single use tips (fig. 8/4)
BS-040118-AK
Hydrophobic filter
BS-040108-S25
Aspiration tip, 200 µL, 1000 pcs. (fig. 8/2)
BS-000001-S04
Aspiration tip, 1000 µL, 1000 pcs. (fig. 8/3)
BS-000001-S05
Aspiration tip, 8 channels, for MA-8 (fig. 8/1A)
BS-040108-S24
Trapping flask, 2 L, polypropylene, with lid, inserts and sealing ring
BS-040120-S11
Tubing and fitting set
BS-040120-S14
Sensor cable
BS-040120-S12

Edition 1.-4.03 13
1
2
3
4
5
6 7 8 9 10
Figure 8. Optional accessories and replacement parts

14 Edition 1.-4.03
8. Chemical compatibility
8.1 Different aerosols and vapours from volatile and hazardous agents can have a dam-
aging effect on the specific parts of the units. Please see below for FTA-1 and FTA-
2i material compatibility tables 1 and 2 with different common laboratory chemicals.
It is required to use the FTA-2i pump’s exhaust (fig. 7/3) by connecting it to the means
of required filtration through a tube or, alternatively, by placing the unit in a fume hood
when working with volatile and hazardous agents. FTA-1 does not have pump ex-
haust connector available; therefore, it is obligatory to place the unit in a fume hood.
It is suggested to test the compatibility of related parts to a specific chemical prior to
extensive use. Biosan does not warrant that the data in the tables is correct or com-
prehensive and that any material is fit for any purpose.
8.2 Resistance grades used in tables:
•1(Excellent) –at ambient temperature and pressure, the material should not be affected.
•2(Good) –the material is slightly affected but not to the point of being unsuitable.
•3(Fair) –a degree of reaction that is generally considered unsuitable and should not
be used.
•4(Severe) indicates that the material should not be used under any circumstances.
Table 1. FTA-1 chemical resistance chart
Parts
Material
Formalde-
hyde CH2O
Acetic acid
C2H4O2
Hydrochloric
acid HCl
Sodium
hydroxide
NaOH
Ethanol
C2H5OH
Acetone
(CH3)2CO
Tubing
Silicone
2
2
3
3
1
3
Bottle 1L
Glass
1
1
1
1
1
1
Lid
Polypropylene
1
1
1
1
1
1
Lid seal
NBR
2
3
2
2
2
4
Fittings
Polypropylene
1
1
1
1
1
1
Quick couplings
Ertacetal POM
1
1
3
1
1
1
Housing
Polystyrene
4
3
2
1
2
3
Pump head housing
Polycarbonate
1
2
4
4
2
4
Pump head membrane
EPDM
2
1
2
1
1
1
Pump valve
EPDM
2
1
2
1
1
1
Filter membrane
PTFE
1
1
1
1
1
1

Edition 1.-4.03 15
Table 2. FTA-2i chemical resistance chart
Parts
Material
Formalde-
hyde CH2O
Acetic acid
C2H4O2
Hydrochloric
acid HCl
Sodium
hydroxide
NaOH
Ethanol
C2H5OH
Acetone
(CH3)2CO
Tubing
Silicone
2
2
3
3
1
3
Bottle 2L
Polypropylene
1
1
1
1
1
1
Lid
Polypropylene
1
1
1
1
1
1
Lid seal
TPE
1
3
1
1
1
1
Level detection pins
Stainless steel
3
3
3
2
1
1
Fittings
Polypropylene
1
1
1
1
1
1
Quick couplings
POM
1
1
3
1
1
1
Housing
ABS
2
4
1
1
2
4
Pump head housing
Nylon
4
4
4
3
1
1
Pump head membrane
EPDM
2
1
2
1
1
1
Pump valve
EPDM
2
1
2
1
1
1
Filter membrane
PTFE
1
1
1
1
1
1

16 Edition 1.-4.03
9. Care and maintenance
9.1 Service.
9.1.1 If the unit is disabled (e.g., no aspiration, no reaction to key presses, etc.) or requires
maintenance, consult the troubleshooting table in 9.5. If the problem is not covered,
disconnect the unit from the mains and contact Biosan or your local Biosan repre-
sentative.
9.1.2 All maintenance and repair operations (except listed below) must be performed only
by qualified and specially trained personnel.
9.1.3 Operating integrity check. If the unit follows the procedure described in section Op-
eration, then no additional checks are required.
9.2 Cleaning and disinfection.
9.2.1 Use mild soap and water with a soft cloth or sponge for cleaning the exterior. Rinse
remaining washing solution with distilled water. Wipe dry the excess water with clean,
soft cloth or sponge.
9.2.2 To disinfect the plastic and glass parts, use 75% ethanol or DNA/RNA removing so-
lution (e.g., Biosan PDS-250). After disinfecting, wipe the surfaces dry.
9.2.3 Silicone and fittings. Detach and disassemble the tubing. Use only distilled water, no
less than 0.5 L, and air dry after rinsing.
9.2.4 Autoclaving procedures.
Lid, fittings, tubing
Flask
Hydrophobic filter, cables,
base pump
FTA-1
121°C and 1 atm.,
15 min.
121°C and 1 atm., 55 min.
Do not autoclave
FTA-2i
121°C and 1 atm., 15 min.
Caution!
Do not put a closed flask in the autoclave, as it might irreversibly deform.
Completely disengage the thread on the cap.
Note.
Hydrophobic filter and sensor cable are sensitive to moisture and are
not autoclavable. Tubing is made of silicone and deteriorate after fre-
quent autoclaving and should be replaced.
9.3 Tubing replacement. Change the silicone tubing every 6 months.
9.4 Filter replacement. Replace the filter as soon as it accumulates visible traces of dirt,
or when the liquid is sucked into it. Maximum working time for the filter is 30 days.
When working with infected materials, replace the filter immediately after finishing the
procedure to prevent the spread of infection.
9.4.1 Changing procedure. Remove the filter (fig. 1/2 or 6/1) by detaching it from adapters.
Insert a new filter and reconnect the adapters.
Caution!
Observe the orientation of the filter:
•For model FTA-1, the IN marking should be facing towards the unit (figure 1).
•For model FTA-2i, the IN marking should be facing away from the unit (figure 6).

Edition 1.-4.03 17
9.5 Troubleshooting.
Problem
Possible reason
Troubleshooting
Unit not working
No power
Check external power supply and con-
nector
(FTA-2i) Trapping flask full
Sensor shut down the motor. Empty the
trapping flask according to 5.6.
(FTA-2i) Sensor not connected
properly
Check sensor cable and attachments
Aspiration slow or
absent
(FTA-2i) Aspiration intensity set to
small
Turn the regulator knob clockwise to in-
crease aspiration.
Breach in the system
Check all the tubing and fittings. Tightly
close the lid of the unit
Dirt in the tubing
Clean the tubing, see 8.3.
Liquid in the filter
Replace the filter
(FTA-2i) Sensor
activated, but the
flask is not full
Foam in the trapping flask activated
the sensor
Do not use the unit with a foaming liquid
or use antifoaming agent.
9.6 Disposal. Disposal of the appliance requires special precautions and must be carried
out at an appropriate disposal site, separate from normal household waste. To prevent
pollution of the environment, all waste resulting from the disposal of the product must
be collected and disposed of in the country of use, in accordance with the applicable
requirements for the handling of electronic waste.
10. Storage and transportation
10.1 Store and transport the unit in a horizontal position (see package label) at ambient
temperatures between -20°C and +60°C and maximum relative humidity of 80%.
10.2 After transportation or storage and before connecting it to the electric circuit, keep the
unit under room temperature for 2-3 hrs.
10.3 For extended storage, disassemble and clean according to 9.3. Empty the trap flask.
The unit does not require special procedures.

18 Edition 1.-4.03
11. Warranty
11.1 The Manufacturer guarantees the compliance of the unit with the requirements of
Specifications, provided the Customer follows the operation, storage and transporta-
tion instructions.
11.2 The warranted service life of the unit from the date of its delivery to the Customer is
24 months. For extended warranty, see 11.6.
11.3 Warranty for the unit does not cover optional accessories or replacement parts listed
in 7.2.
11.4 Warranty covers only the units transported in the original package.
11.5 If any manufacturing defects are discovered by the Customer, an unsatisfactory
equipment report shall be compiled, certified and sent to the local distributor address.
To obtain the claim form, visit Technical support page on our website at link below.
11.6 Extended warranty.
•For FTA-2i, the Premium class model, one year of extended warranty is available
free of charge after registration, during 6 months from the date of sale. Online regis-
tration form can be found in section Warranty registration on our website at the link
below.
•For FTA-1, the Basic Plus class model, extended warranty is a paid service. Contact
your local Biosan representative or our service department through the Technical
support section on our website at the link below.
11.7 Description of the classes of our products is available in the Product class descrip-
tion section on our website at the link below.
Technical support
Registration
Product class description
biosan.lv/en/support
biosan.lv/register-en
biosan.lv/classes-en
11.8 The following information will be required in the event that warranty or post-warranty
service comes necessary. Complete the table below and retain for your records.
Model
Serial number
Date of sale
FTA-1, FTA-2i,
Aspirators with trap flask
11.9 Production date. Production date is placed in the serial number, on the label of the
unit. Serial number consists of14 digits styled XXXXXXYYMMZZZZ, where XXXXXX
is model code, YY and MM –year and month of production, ZZZZ –unit number.

Edition 1.-4.03 19
12. EU Declaration of conformity
12.1 Aspirators with trap flasks FTA-1 and FTA-2i are in conformity with the following rel-
evant Union legislations:
LVD 2014/35/EU
LVS EN 61010-1:2011 Safety requirements for electrical equipment for
measurement, control, and laboratory use. General requirements.
EMC 2014/30/EU
LVS EN 61326-1:2013 Electrical equipment for measurement, control and
laboratory use. EMC requirements.
General requirements.
RoHS3 2015/863/EU
Directive on the restriction of the use of certain hazardous substances in
electrical and electronic equipment.
WEEE 2012/19/EU
Directive on waste electrical and electronic equipment.
12.2 Declaration of Conformity is available for download on the page for the relevant model
on our website by links below, in the Downloads section:
FTA-1 FTA-2i
Other manuals for FTA-1
2
This manual suits for next models
1
Table of contents
Other Biosan Medical Equipment manuals