Biosense BREATH KETONE METER User manual
Patient Guide
Biosense Device and App
The Biosense® devi e measures breath a etone or BrA e, whi h is produ ed as a byprodu t of
fatty a id oxidation and the resulting formation of energy-yielding ketone bodies. Ketones are
produ ed during times of glu ose insuf ien y and are released into the blood to be taken up
by tissues to be metabolized for energy. The on entration of a etone in the breath indi ates
your level of ketone produ tion resulting from fatty a id oxidation. The devi e represents
breath a etone in s aled units alled ACEs, from 0-40. The higher your ACEs, the more
ketones are being produ ed. Ketosis is the term used to des ribe a state in whi h there is an
a umulation of ketones in the body tissues and uids.
The Biosense Device
There are three different ketone bodies: Acetoacetate (AcAc), Beta-hydroxybutyrate (BhB) and
Acetone (BrAce) Ketones represent a third class of energy substrates for your metabolism
Under conditions of insufcient glucose, your liver converts fats into the ketones AcAc and BhB
for energy BrAce is a direct by-product of AcAc and a volatile organic compound measurable in
the breath AcAc and BhB both circulate in the blood and can interconvert within cells Urinary
dipsticks measure AcAc as unused AcAc is excreted in the urine Urine tests provide a range of
ketone levels rather than a discrete value Further, AcAc levels in the urine decline after several
weeks of keto-adaptation limiting their utility to track nutritional ketosis over time BhB measured
in venous blood provide the highest level of accuracy but must be done in a lab At-home BhB
nger prick tests measure BhB in capillary blood but are inconvenient and costly over time
Three types of Ketone Bodies and Why it Matters
As ketone levels often vary greatly throughout the day, a single ketone measurement provides a
limited view of an individual's metabolic state To capture cumulative progress over time, our
clinical study calculated a daily area under the curve (AUC ) The AUC is a summary of the
accumulated depth and duration of nutritional ketosis each day It answers the question, "how
deeply and for how long have I been in ketosis?" with a single number Our clinical data
showed a very high correlation between breath and blood AUCs (R2 = 0 83) when both
measurements were taken 3 times daily Stated differently, using a breath or a blood device to
take multiple measurements per day captures the high variability of daily ketone levels and
provides very similar indications of overall ketone activity
Measuring breath acetone allows you to track if you are producing ketones and indicates your
level of fatty acid oxidation Ketosis may result in many health benets for you such as, fat loss,
glucose control, blood pressure regulation, improved cognitive function, and has been studied
for its role in increasing longevity Ketosis is inuenced primarily by diet and fasting Exercise,
stress (increasing cortisol and insulin), and sleep have also been shown to impact levels of
ketosis As diet plays a primary role in fatty acid oxidation and ketone formation, it is important
for you to follow a dietary plan resulting in lowering glucose and increasing ketones Research
has shown a variety of dietary plans to be effective, including carbohydrate restriction, calorie-
restriction, and more Though following a plan may be easy for some patients, people often
react very differently to the same lifestyle change, making it challenging to identify which food
choices and in which quantities affect each patient's ketone production rate Therefore,
measuring matters When you track ketones, it allows your practitioner to be better informed
about how you react to interventions in not only diet, but also exercise, stress and sleep
Why Measure Multiple Times Daily
What Breath Acetone Measurements Tell You
About Your Health
Downloading the app
Pairing device to phone
Click one of the links below to download the iosense app to
your mobile device, or search iosense in the app store.
Turn on the Biosense device and enable Bluetooth on your
phone. Open the Biosense app and turn on your Biosense
by pressing the check button for three seconds.
If the device is not on this screen, the device can still be
paired as long as it is not asleep. Select “Pair a Device”,
then select your device from the device list. Your device
name will appear as “B” or “BiosenseXXXX”(example:
Biosense1234). If you have successfully paired, you will see
a message that says Device Connected. After the Biosense
device and app are manually paired for the rst time, they
will automatically connect each time the app is open and
the Biosense device is on and nearby
iosense has a simple two-button interface and an onboard
LED screen that guides the user through the measurement
process. To begin taking a measurement, the rst thing you
need to do is power on your device. To do so, press and
hold the check button until you see the blue iosense logo.
The optimal blow technique consists of a normal inhale and
then a full exhale with medium force (think blowing up a
balloon or blowing a bubble).The device waits until the end
of the exhalation to begin measuring. Keep breathing until
you hear two short vibrations from the device. Finally, after a
brief analysis period (around 15 seconds), the measurement
result is displayed on the device screen. The result is also
placed on a color scale that indicates where the your fat
burn rate lies on the overall spectrum of ketosis.
Once the device is on, it will immediately enter into a preparation stage as
indicated by the Preparing Device screen, vibrating as it warms up and
calibrates. This process can take up to 120 seconds to warm up. After
device preparation is complete, you will see a new screen that says Press
Start. At this point, you can press the check button to take a
measurement. You will then be prompted to blow into the device.
Using the device
Hi tory: View the previous 10 measurements or delete
measurements stored on the device. Biosense can store a
maximum of 100 measurements
Set Time: Manually set the device clock. If you pair to the
app, the app will automatically set the device clock and
manual time setting is not necessary
Info: View device information like serial number, rmware
version, sensor status and microphone status. If the sensor
status shows CLEAN, run a clean cycle. If the mic status
shows FAIL, contact
Clean: Run an extended clean cycle. A red dot next to the
settings gear indicates a sensor clean is recommended
Home: Navigate back to the device Home Screen.
support@mybiosense.com
Device etting
Charging your device
Cleaning your device
Charge your Biosense device using the provided Micro USB charging cable and an approved LPS
power supply (maximum rating 5V/1A). Your device needs two hours for a full charge. When your
device is charging, the LED underneath the mouthpiece will glow red. The red LED light will
automatically shut off once the device has reached a full charge.
Clean your Biosense device once a week. Remove the mouthpiece from the device before
cleaning by gently squeezing its base and pulling it away from the device. Use a cloth and water
to clean your mouthpiece. Do not use cleaning wipes that contain alcohol and do not clean the
white lter on the device.
Removeable
Mouthpiece
Display
Screen
START
Button
SETTING
Button
When your Biosense device and phone are proper y
paired, there are two ways to sync measurements to
the app. Keep your Biosense app open to automatica y
sync your resu ts during a measurement. To maintain
accurate breath ketone readings, ACEs shou d be
measured severa times a day
Syncing readings to the app
Share results
You have the option to share your resu ts with your practitioner by c icking the share button in
the upper right corner of the app. Sharing resu ts and being honest about your experience is an
integra component of the Biosense program. It a ows both you and your provider to measure
success against dai y habits.
The pairing icon on the top
right corner of the home
screen indicates whether
you are connected (blue) or
not connected to Biosense
(gray). You can tap on your
pairing icon to connect
Biosense if it does not
connect automatically
NOT connected to Biosense
CONNECTED to Biosense
Pairing Icon
Using the Biosense App
The answers,
“how long and deeply you have
een in ketosis,” estimated
from your ACEs measurements.
The Average
(week, month, and year time
frames) only takes into account
the days when you measured
with Biosense.
Ketone Dose
Ketone Dose
The popup tells you your
most recent measurement,
your level of ketosis, and
associated potential
health enets.
ACEs
ACEs
The Biosense app is designed to provide you with
iomarker feed ack and insights on a daily asis
Real-time biofeedback so you understand how
diet, exercise, and fasting impacts fat urn levels.
You can monitor your body’s increasing fat burn levels to
opt m ze the r fast
Pos t ve re nforcement to keep you motivated to get into
and stay in a fat burn state.
Easy to access reports that help you track their progress and
help you guide treatment decisions
Biosense is simple and easy to use. Follow this quick start guide to get familiar with the
Biosense device.
Biosense has a simple two-button interface and an onboard LED screen that guides you
through the measurement process. To begin taking a measurement, the rst thing you need to
do is power on your device. To do so, press and hold the check button until you see the blue
Biosense logo.
Once the device is on, it will immediately enter into a preparation stage as indicated by the
Preparing Device screen, vibrating as it warms up and calibrates. This process can take up to
120 seconds.
After device preparation is complete, you will see a new screen that says Press Start. At this
point, you can press the check button to take a measurement. You will then be prompted to
blow into the device.
How do I use the Biosense device?
Q/A 1 USING YOUR BIOSENSE BREATH KETONE METER
Question
& Answers
Bi sense readings are rep rted in units called ACEs, a unique unit f breath acet ne ranging fr m
0 (n ket nes) t 40 (high ket nes). When referring t target ACEs, there is n ne-size-ts-all
answer because the target ACEs vary depending n y ur g als.
H wever, with readings that g up t 40, this scale needs t be further expanded up n. While a
ket ne reading f >5 is the standard g al f r weight l ss patients, even higher ket ne levels play
an essential r le in tapping int the benets f ket sis bey nd weight l ss.
What should my target ACEs be?
Q/A 2 TARGET ACES: DIFFERENT DEPENDING ON YOUR GOALS
Mon Tue Wed Thu Fri Sat Sun
7
19 20
27
38
20
6
4
3
2
1
0
Q/A 2
1-4 ACEs 5+ ACEs
10+ACEs 15+ ACEs
Lo Ketones
Still burning glucose as primary
fuel.
Burning fat for fuel
Lowering blood sugar
Decreasing belly fat
Reducing blood pressure
Improving lipid profiles.
Increasing mental clarity
Easing inflammation
Enhancing brain health.
Regenerating cells
Enhancing health and energy.
Weight Loss
Still burning glucose as primary
fuel.
Burning fat for fuel
Lowering blood sugar
Decreasing belly fat
Reducing blood pressure
Improving lipid profiles.
Increasing mental clarity
Easing inflammation
Enhancing brain health.
Regenerating cells
Enhancing health and energy.
Cognitive & Anti-Inammatory
Still burning glucose as primary
fuel.
Burning fat for fuel
Lowering blood sugar
Decreasing belly fat
Reducing blood pressure
Improving lipid profiles.
Increasing mental clarity
Easing inflammation
Enhancing brain health.
Regenerating cells
Enhancing health and energy.
Healthspan & Autophagy
Still burning glucose as primary
fuel.
Burning fat for fuel
Lowering blood sugar
Decreasing belly fat
Reducing blood pressure
Improving lipid profiles.
Increasing mental clarity
Easing inflammation
Enhancing brain health.
Regenerating cells
Enhancing health and energy.
Understanding the ACEs unit
Biosense ketone measure results are reported in units called ACEs. Tap on the below arrows
to better understand potential health benets associated with specic ACE ranges.
To ma nta n accurate breath ketone read ngs, you should measure your ACEs at least three times
a day.
Breath and blood ketones both vary dramat cally over the course of a day. The patterns of ketone
level changes are dependent upon the spec c day and the nd v dual, what they eat, how they
exerc se, etc. Although breath and blood ketones tend to r se and fall together, there s often a
t me offset between changes n blood ketones and changes n breath ketones. Speak w th your
Pract t oner for more nformat on regard ng offset t mes
ACE Measurement Frequency
Q/A 3 TESTING FREQUENCY: UNDERSTANDING KETONE MEASUREMENTS
Benets of frequent testing for users
Mon tor ng your ketones prov des you real-t me feedback about how your d etary and l festyle
cho ces affect your ketones. The B osense breath ketone meter prov des a much t ghter feedback
loop than the we ght scale, wh ch s the trad t onal feedback tool for obes ty management and
l festyle change programs. W th B osense, you can get feedback about the effect of a part cular
cho ce on the r fat burn rate n as l ttle as one hour. Th s creates a strong psycholog cal connect on
between the act ons you are tak ng and the results you observe, a connect on that strengthens as
feedback frequency ncreases. As you learn more about your body and the effects of your
cho ces, you w ll become ncreas ngly nvested n your own success.
When you are beginning a new health plan targeting etosis, it is essential to consider the
following general guidelines for interpreting your results. It is not uncommon to remain in the 0-3
range for the rst few days while glycogen stores are depleted.
0-2 ACEs – not in etosis. ACEs readings of 0-2 represent the bac ground fat burning rate
present in all individuals regardless of diet or lifestyle. Even individuals who are eating a high-
calorie or high carbohydrate diet can have an ACEs reading of 2.
3 ACEs – slightly elevated etones. An ACEs reading of 3 is the lowest reading that can be
associated with elevated etones
4-5 ACEs – transitioning into etosis. Ketones are elevated, and your patients/clients are
transitioning into a primarily fat-burning state. This is the point at which glycogen stores are being
depleted in the liver
>5 ACEs – denitively in etosis. ACEs readings above 5 represent an even deeper state of fat
burn. Some individuals, particularly those with diabetes and metabolic disease, may not be able to
achieve ACEs levels above 4-5. For this reason, a patient with a maximum etone level of 4-5
ACEs should be considered successful.
How do ACE readings relate to weight loss?
Q/A 4 THE CONNECTION BETWEEN ACE READINGS AND WEIGHT LOSS
While it is quite common for those already racticing low-carb eating and/or racticing fasting
to achieve ketone levels in the 10-40 ACEs range, atients new to these dietary changes will
often begin with ketone levels less than 5 ACEs. Low ketone readings are not only normal when
rst beginning your Biosense journey, they are to be ex ected.
Glucose is the rst metabolite the body will use to convert to energy via the TCA cycle to
roduce ATP. Once glucose is utilized, the liver will metabolize its stored glycogen to convert to
energy. Once the liver's glycogen stores are de leted, the body will begin to convert stored
triglycerides into free fatty acids (FFA) and oxidize the FFA's to enter into the TCA cycle to
roduce ATP. Ketone bodies are formed as a by roduct of FFA oxidation
Basically, the body needs to burn through all glycogen stores before it begins to burn fat as
energy, causing ketone levels to rise. This rocess can take u to a few days.
What do consistently low ketone
readings indicate?
Q/A 5 GLYCOGEN: ESSENTIAL TO UNDERSTANDING A LOW KETONE READING
It is im ortant to note that consistency is key to remaining
in ketosis. For exam le, when an individual eats a high
calorie or high-carbohydrate meal, their glycogen lls back
u . If they were to then resume their diet after the high
calorie meal, they will have to rst burn through their
newly stored glycogen before they will start to see their
ketones rise. This inter lay between glycogen and fat
metabolism is the single most im ortant conce t to
consider when inter reting ketone data. It is not
uncommon to remain in the 0-3 range for the rst few days
while your glycogen stores are de leted
Factors that affect ketone levels include, but are not limited to:
Facto s that affect an ACEs measu ement
Q/A 6 FACTORS THAT AFFECT KETONE LEVELS
1. Calorie intake
• ACE levels increase with fasting and caloric
restriction
• Conversely, ACEs drop, often suddenly, after
an excessively high calorie meal
2. Carbohydrate intak
• ACEs increase as dietary carbohydrates are
reduced
• Conversely, ACEs drop, often suddenly, after
an excessively high carbohydrate meal
5. Alcoho
• Consuming alcohol will affect ACE levels. It i
recommended that you wait at least 5-6 hours
or wait until the next day post-consumption to
measure ACEs
3. Exercise duration and intensit
• During exercise, the body rst will deplete
stored glucose in the blood for energy, and then
utilize stored glycogen in the liver. Once glucose
and glycogen stores are depleted, the body uses
ketones for energy.
• The short-term effect of exercise on ACE
levels depends on whether ketones are elevated
or not before exercise. If ketones are elevated
before exercise, they often drop temporarily
after exercise before rising again (this is due to
the use of ketones during exercise and a
4
.
S
tress le
v
el
• High stress levels increase cortisol production
raising glucose production in the liver and
lowering ketone levels
• Stress can be indirectly affected through
factors like sleep. Speak to your patients about
good sleep hygiene and stress management
techni
q
ues as they directly and indirectly affect
fat oxidation and ketone production
temporary spike in blood glucose caused by
cortisol, which is released during exercise
)
. If
ketones are low before exercise, they tend to
simply rise after exercise, without experiencing
a drop.
Take ACE measurements about an hour post-
exercise to allow time for the ketones to rise again
after a temporary drop.
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