biosystems X-CLARITY User manual

X-CLARITY™
Tissue Clearing System
User Manual
www.logosbio.com
LBSM-0005
V160726-01

1
The contents of this document may be changed in the future without advance notice.
All copyrights of this manual are owned by Logos Biosystems.
The X-CLARITY™ Tissue Clearing System is a set of laboratory electrical instruments for scientific research use only.
It is not a medical, therapeutic, or in vitro diagnostics device.
The WEEE (Waste Electrical and Electronic Equipment) symbol indicates that users of this system have the
responsibility of returning and disposing of WEEE in an environmentally friendly manner. Follow the waste
ordinances of your region for proper disposal provisions.
The CE mark indicates that this system conforms to all applicable European Community provisions for which
this marking is required. Users must be aware of and follow the conditions described in this manual for
operating the system. The protection provided by the system may be impaired if the system is used in a manner
not specified by this manual.
Protective earth (Ground)
FCC
COMPLIANCE
This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part
15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference
when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate
radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause
harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause
harmful interference in which case the user will be required to correct the interference at the user’s own expense.

2
Table of Contents
Table of Contents.......................................................................................................................................................................................2
Safety Information .....................................................................................................................................................................................3
Environmental Conditions for Operation .................................................................................................................................................3
1 Introduction .....................................................................................................................................................................................4
1.1 Product Overview ..................................................................................................................................................................4
1.2 Product Contents...................................................................................................................................................................4
1.3 Product Specifications...........................................................................................................................................................5
1.3.1 X-CLARITY™ETC Chamber............................................................................................................................................5
1.3.2 X-CLARITY™ETC Controller ..........................................................................................................................................5
1.3.3 X-CLARITY™Pump ........................................................................................................................................................5
1.3.4 X-CLARITY™Reservoir ..................................................................................................................................................5
2 Product Description.........................................................................................................................................................................6
2.1 X-CLARITY™ETC Chamber......................................................................................................................................................6
2.2 X-CLARITY™ETC Controller ....................................................................................................................................................7
2.3 X-CLARITY™Pump...................................................................................................................................................................8
2.4 X-CLARITY™Reservoir.............................................................................................................................................................8
3 Installation........................................................................................................................................................................................9
3.1 Multi-Cable: ETC Controller and the ETC Chamber .............................................................................................................9
3.2 Temperature Probe Connector: Temperature Probe and the ETC Controller...................................................................9
3.3 Peristaltic Pump Tube: Reservoir Outlet and the ETC Chamber Inlet.................................................................................9
3.4 Snap-Lock Connector Tube: ETC Chamber Outlet and the Reservoir Inlet ......................................................................10
3.5 Power Cords.........................................................................................................................................................................10
4 Running...........................................................................................................................................................................................11
4.1 Buffer Flow...........................................................................................................................................................................11
4.2 Sample Insertion..................................................................................................................................................................11
4.3 Tissue Clearing .....................................................................................................................................................................12
4.4 Chamber Opening................................................................................................................................................................13
4.5 Buffer Replacement.............................................................................................................................................................13
5 Maintenance and Troubleshooting...............................................................................................................................................14
5.1 ETC Chamber Cleaning ........................................................................................................................................................14
5.2 Snap-Lock Connector Cleaning ...........................................................................................................................................14
5.3 Troubleshooting...................................................................................................................................................................15
5.4 Error Codes ..........................................................................................................................................................................16
6 Ordering Information.....................................................................................................................................................................17
7 Purchaser Notification...................................................................................................................................................................18

3
Safety Information
Before using this system, read this manual carefully to ensure that you know how to operate it safely and correctly. Keep this
manual in an easily accessible location for future reference. Use the system only as specified.
1. Avoid electric shock while operating the instruments. Do not touch the components with wet hands.
2. Operate the instrument in the conditions described in the Environmental Conditions for Operation.
3. Wear proper personal protective equipment (PPE) when handling Electrophoretic Tissue Clearing Solution or X-CLARITY™
Mounting Solution to avoid exposure.
4. Use only components provided or authorized by Logos Biosystems.
5. Make sure that the input voltage is compatible with the X-CLARITY™ETC Controller power supply before use.
6. Use the power cord and AC adapter provided. Ensure that the power cords are firmly plugged into the power source and
the X-CLARITY™ETC Controller.
7. Turn the X-CLARITY™ETC Controller on only after connecting the power cord to it and the power source. Always turn the X-
CLARITY™ETC Controller off before disconnecting the power cord and/or moving the instrument.
8. Turn the power off before opening the X-CLARITY™ETC Chamber.
9. Carefully monitor tube connections. Replace tubes if leakage occurs.
10. Always use the pump to fill or drain the X-CLARITY™ETC Chamber. Do not pour liquids in or out directly.
11. Use the recommended volume of Electrophoretic Tissue Clearing Solution in the provided X-CLARITY™Reservoir.
12. Do not use organic solvents other than the Electrophoretic Tissue Clearing Solution described in this manual. Different
solvents may cause permanent and irreparable damage.
13. If components emit smoke, disconnect the power cord immediately from the power source2 and contact a local distributor
or Logos Biosystems.
14. Do not disassemble the components in any event. Disassembling the components invalidates the warranty. If a device
malfunctions, contact a local distributor or Logos Biosystems.
Environmental Conditions for Operation
Operating Power
100 ‒ 120 VAC, 2.2 A / 200 ‒ 240 VAC, 1.1 A
Frequency
50/60 Hz
Installation Site
Indoor use only
Operating Temperature
10 ‒ 30°C
Maximum Relative Humidity
20 ‒ 70%
Altitude
≤ 2,000 m
Pollution Degree
2

4
1Introduction
1.1 Product Overview
CLARITY is a method developed by scientists at Stanford University that produces structurally sound and transparent whole
tissues ready for multiple rounds of antibody labeling and imaging. The method has opened up a world of possibilities, from
tracing neural circuitry to exploring the relationship between structure and function with a global perspective.
With the CLARITY method, the biomolecules in intact tissues are covalently linked to a sturdy hydrogel network, preserving
molecular information and structural integrity. Lipid bilayers are broken up through electrophoresis in the presence of ionic
detergents, leaving behind a stable and transparent tissue-hydrogel hybrid that is chemically accessible for molecular
phenotyping.
Logos Biosystems licensed and independently developed the CLARITY technology to make significant improvements for tissue
clearing efficiency and reproducibility. The X-CLARITY™Tissue Clearing System is an all-in-one, easy-to-use solution for
electrophoretic tissue clearing. Its unique design accelerates the removal of lipids from tissues in a highly efficient manner.
Complicated wires are combined into a single cable for power supply and control. Improved electrodes generate a uniform
electric field that eliminates uneven voltages and currents. A timer allows precise control over the clearing time by automatically
turning off the current at the end of the allotted time. An active cooling system minimizes the probability of artifacts arising due
to poor temperature control. Buffer circulation ensures consistent buffering capacity, temperature control, and elimination of
electrophoretic byproducts. This advanced system ensures that tissue clearing occurs efficiently and rapidly.
1.2 Product Contents
The X-CLARITY™Tissue Clearing System contains the following components:
Component
Quantity
X-CLARITY™ETC Chamber
1 unit
X-CLARITY™ETC Controller
1 unit
X-CLARITY™Pump
1 unit
X-CLARITY™Reservoir
1 unit
Multi-Cable
1 unit
Power Cord
2 units
Snap-Lock Connector Tube*
1 unit
Peristaltic Pump Tube
1 unit
Container Holder for 1 Tissue Container
1 unit
Tissue Container
5 units
Electrophoretic Tissue Clearing Solution
12 x 1 L
*Assembly required
Upon receiving the product package, inspect its contents to ensure that all parts have been included and that no damage has
occurred during shipping. The warranty does not cover damage that may occur during shipping and handling. Any damage claims
must be filed with the carrier.

5
1.3 Product Specifications
1.3.1 X-CLARITY™ETC Chamber
Instrument Type
Electrophoretic chamber
External Dimensions (W x D x H)
176 mm x 128 mm x 154 mm
Internal Dimensions (W x D x H)
57 mm x 30 mm x 93 mm
Weight
2.8 kg
Note: Power to the X-CLARITY™ETC Chamber is supplied by the X-CLARITY™ETC Controller via the multi-cable.
1.3.2 X-CLARITY™ETC Controller
Instrument Type
Power supply and control
Dimensions (W x D x H)
180 mm x 340 mm x 200 mm
Weight
5.1 kg
Applicable Power
AC 100-240 V, 50/60 Hz
Power Consumption
210 W (including the X-CLARITY™ ETC Chamber)
1.3.3 X-CLARITY™Pump
Instrument Type
Peristaltic pump
Dimensions (W x D x H)
232 mm × 142 mm × 149 mm
Weight
2.4 kg
Speed
0.1-100.0 rpm
Speed Precision
0.1 rpm
Applicable Power
AC 100-240V, 50/60 Hz
Power Consumption
30 W
Note: Please see the separately supplied user manual for more information.
1.3.4 X-CLARITY™Reservoir
Material
Glass
Total Volume
2 L
Recommended Buffer Volume
1.2 L

6
2Product Description
The X-CLARITY™ Tissue Clearing System consists of the X-CLARITY™ ETC Chamber, X-CLARITY™ ETC Controller, X-CLARITY™ Pump,
and X-CLARITY™ Reservoir.
2.1 X-CLARITY™ETC Chamber
The X-CLARITY™ ETC Chamber is an electrophoretic device that applies an electric current to tissue samples placed in the
chamber. Plated electrodes produce a uniform electric field for consistent clearing. The chamber inlet and outlet allow for buffer
circulation, which eliminates the loss of buffering capacity, reduces the risk of overheating, and removes electrophoretic
byproducts. An integrated cooling system dissipates the heat generated during electrophoresis. A magnetic safety switch detects
the opening and closing of the safety cover. If the cover is open, the X-CLARITY™ ETC Controller cannot supply power to the ETC
Chamber.

7
2.2 X-CLARITY™ETC Controller
The X-CLARITY™ETC Controller controls the power, temperature, and safety for the X-CLARITY™ETC Chamber through one cable,
eliminating the need for dangerous and messy wiring.
To adjust values for the current, temperature, or timer, push the SET button located on the front panel of the ETC Controller.
The display will blink on in the following order when you continue to push the SET button: Current Temperature Timer.
Use the UP and DOWN buttons to adjust to the desired values. Press and hold down the UP/DOWN button to speed up
selection. Push the SET button again to apply the setting.
The ETC Controller ranges are:
Range
Increment
Voltage (V)
Not adjustable, max 60
N/A
Current (A)
0.2-1.5
0.1
Temperature (°C )
35-60
1
Timer (hours)
0.5-99.5, 100-999, CL
0.5, 1.0, CL
The timer increases in increments of 0.5 hours from 0-99.5 hours and then in 1 hour increments from 100-999 hours.
The “– ––“ setting is for continuous running of the system. The timer also includes a cleaning mode (CL), to use when cleaning
the ETC Chamber (see Section 5.1: ETC Chamber Cleaning). The timer goes from 999 CL –––0.5.
The X-CLARITY™Tissue Clearing System only works in constant current mode. We found that constant current mode leads to
more consistent tissue clearing. The recommended constant current value is 1.5 A. Changing the current value may affect the
time and/or efficiency of clearing and may require further optimization.

8
2.3 X-CLARITY™Pump
The peristaltic pump circulates the buffer to maintain consistent buffering capacity and temperature. Always use the pump to fill
or drain the ETC Chamber. Do not pour liquids in directly.
Depending on how the pump has been oriented in relation to the ETC Chamber, run the pump in a clockwise (CW) or
counterclockwise (CCW) direction to either supply or drain the chamber. The Prime (▶▶) button runs the pump at maximum
speed. At 30 rpm, the pump moves approximately 50 mL per minute.
See the separately supplied pump manual for more information.
2.4 X-CLARITY™Reservoir
It is important to circulate Electrophoretic Tissue Clearing Solution during tissue clearing to maintain buffering capacity, control
buffer temperature, and remove electrophoretic byproducts from the ETC Chamber. The temperature probe attached to the
reservoir cap is connected to ETC Controller and monitors the temperature of the clearing solution.

9
3Installation
Place the components of the X-CLARITY™Tissue Clearing System on a clean and flat surface devoid of vibration. Leave sufficient
space around the ETC Chamber for the cooling fan to work properly.
3.1 Multi-Cable: ETC Controller and the ETC Chamber
Connect the ends of the multi-cable to the ports on the rear panels of the ETC Controller and Chamber. Check the homing
grooves and the orientation of the pins on the ends of the multi-cable for proper orientation. Fasten the nuts tightly to ensure a
tight connection.
3.2 Temperature Probe Connector: Temperature Probe and the ETC
Controller
Screw the cap onto the reservoir. Make sure the temperature probe and reservoir outlet tube are both inside the reservoir.
Plug the temperature probe connector into the temperature probe port on the rear panel of the ETC Controller. Check the
homing groove on the end of the connector and the thickness of the pins in the port for proper orientation.
3.3 Peristaltic Pump Tube: Reservoir Outlet and the ETC Chamber Inlet
Attach one end of the peristaltic pump tube to the ETC Chamber inlet located on the front panel of the ETC Chamber. Attach the
other end to the reservoir outlet (the reservoir outlet is connected to tubing that extends into the reservoir). Make sure there is
a tight seal at each connection to prevent buffer leakage.
Pull the pump head lever to the left to open the compression block. Lift the clamps on both sides of the pump head. Load the
middle of the peristaltic pump tube into the pump head. Make sure the tube is between the rollers and the compression block.
Release the clamps. Pull the pump head lever to the right to close the compression block.
The provided peristaltic pump tube has a tubing life of 3,000 hours. Only use tubes provided or approved by Logos Biosystems.

10
3.4 Snap-Lock Connector Tube: ETC Chamber Outlet and the Reservoir Inlet
Screw the chamber cap onto the ETC Chamber. Close the safety cover.
Attach the plastic head of the snap-lock connector tube to the chamber outlet protruding from the chamber cap. You will feel
and hear a click when it has attached properly. Attach the other end of the snap-lock connector tube to the reservoir inlet. Make
sure there is a tight seal to prevent buffer leakage.
Do not open or close the ETC Chamber safety cover when the snap-lock connector tube is attached to the ETC Chamber outlet.
Make the connections as tight as possible to prevent buffer leakage and make sure the tubes do not vent.
3.5 Power Cords
Plug in the power cords to the pump and ETC Controller. Connect both to power sources.

11
4Running
4.1 Buffer Flow
Fill the reservoir with 1.2 L Electrophoretic Tissue Clearing Solution. Use fresh Electrophoretic Tissue Clearing Solution with each
run. Reused clearing solution may have lowered buffering capacity, which reduces clearing efficiency. Prolonged use of
Electrophoretic Tissue Clearing Solution may cause electrolysis of the extracted lipids, turning the tissue yellow. Screw on the
reservoir cap. Make sure all tubes are connected securely and properly.
Turn on the pump. Set the pump speed to 30-50 rpm and direction to pump the Electrophoretic Tissue Clearing Solution from
the reservoir to the ETC Chamber. Make sure the buffer is flowing in the right direction: reservoir reservoir outlet 
chamber inlet ETC Chamber. Stop the pump when the ETC Chamber is half full. Do not fill the ETC Chamber to the brim prior
to sample insertion. Always use the pump to fill or drain the ETC Chamber. Do not pour the solution in directly.
4.2 Sample Insertion
Press the stainless steel button on the side of the snap-lock connector to release the tube from the ETC Chamber. Open the
safety cover and unscrew the chamber cap.
Place the hydrogel-embedded tissue sample into the tissue container and put the top on. Insert the tissue container into the
container holder.
Insert the tissue container holder into the chamber (upright).
Screw on the chamber cap tightly. If the chamber cap has been fastened correctly, the silicone ring inside the cap will create a
tight seal, preventing leaks. In case of leakage, please check the silicone ring inside the chamber cap.
Close the safety cover. If the cover is open, the system will not run even if you push the RUN/STOP button.

12
Attach the plastic head of the snap-lock connector tube to the chamber outlet protruding from the cap. You will feel and hear a
click when it has attached properly. NEVER open or close the ETC Chamber safety cover when the tube is still attached to the
chamber outlet. Disconnect the tube from the chamber outlet prior to opening or closing the safety cover by pressing the
stainless steel button on the side of the snap-lock connector.
Ensure all tubes are connected securely and properly.
4.3 Tissue Clearing
Turn on the ETC Controller. Red numbers will appear on the LED displays and the cooling fan within the ETC Chamber will begin
to run. If not, check the power connection.
Use the following settings:
Recommended
Current
1.5 A
Temperature
37°C
Timer
Sample-dependent
Pump speed
30 rpm
Start the pump. Make sure the buffer is flowing in the right direction.
Reservoir  reservoir outlet  chamber inlet  ETC Chamber  chamber outlet  reservoir inlet  Reservoir
Push the RUN/STOP button on the ETC Controller to start clearing. A red halo will appear around the button when the
instrument is running.
In some cases, the current may not initially reach, despite being set at, 1.5 A. However, the current will stabilize to 1.5 A after a
few hours. The current and voltage are dependent on the resistance of the buffer. Depending on the condition of the
Electrophoretic Tissue Clearing Solution, the initial current value may be lower than 1.5 A due to the voltage limit (60 V). If the
system does not reach 1.5 A after a few hours, check the Electrophoretic Tissue Clearing Solution. The tissue container holder
design can also affect the resistance, so use the container holder provided by Logos Biosystems.
As the system runs, you will see bubbles formed by water electrolysis coming from the outlet. A significant amount of heat will
be generated in the ETC Chamber during the tissue clearing process. To eliminate this heat, a cooler has been integrated into the
ETC Chamber. However, temperature control may not be efficient at high external temperatures. Cooling the reservoir with an
additional cooling system is helpful in such cases. Efficient clearing generally occurs at temperatures of 37-60°C.

13
4.4 Chamber Opening
Push the RUN/STOP button on the ETC Controller to stop clearing. Turn the ETC Controller off.
Reverse the direction of the pump (CW to CCW or CCW to CW) to drain the ETC Chamber and the snap-lock connector tube. The
snap-lock connector tube will empty into the ETC Chamber. Make sure the tube is completely empty before disconnecting it
from the ETC Chamber. The buffer will flow out of the chamber inlet back into the reservoir. You can watch the buffer level drop
through the window of the ETC Chamber. When the ETC Chamber is sufficiently drained, turn off the pump.
Press the stainless steel button on the side of the snap-lock connector to release the tube from the ETC Chamber. Gently detach
the snap-lock connector from the chamber outlet. Do not pull the snap-lock connector tube. Open the safety cover and unscrew
the chamber cap.
Check your sample.
4.5 Buffer Replacement
Replace the Electrophoretic Tissue Clearing Solution after every 24 hours.
Push the RUN/STOP button on the ETC Controller to stop clearing. Turn the ETC Controller off.
Reverse the direction of the pump. Turn off the pump when the ETC Chamber and all tubes are empty. Empty the reservoir.
Fill the reservoir with fresh Electrophoretic Tissue Clearing Solution. Screw on the reservoir cap. Make sure all tubes are
connected securely and properly. Reverse the direction of the pump again. Start the pump. Make sure the buffer is flowing in
the right direction.
Continue tissue clearing.

14
5Maintenance and Troubleshooting
5.1 ETC Chamber Cleaning
Wash the ETC Chamber interior with fresh Electrophoretic Tissue Clearing Solution followed by deionized water after each run.
Using the pump, fill the ETC Chamber with 500 mL fresh Electrophoretic Tissue Clearing Solution.
Use the following settings:
Recommended
Current
1.5 A
Temperature
37°C
Timer
CL
Pump speed
30 rpm
To activate cleaning (CL) mode, set the timer to “CL” (when the timer display reads ” –––“, press the DOWN button once). Run
for 1-2 hours. Turn the ETC Controller off before proceeding to the next step.
Reverse the direction of the pump. Turn off the pump when the ETC Chamber and all tubes are empty. Empty the reservoir. Fill
the reservoir with 1 L deionized water. Screw on the reservoir cap. Make sure all tubes are connected securely and properly.
Reverse the direction of the pump again. Start the pump. Make sure the buffer is flowing in the right direction. Press the prime
button to accelerate washing.
The tissue clearing process produces byproducts such as tissue debris and carbonized particles. These dark particles are carbon
aggregates that are generated by the electrolysis of organic materials. Prolonged absorption of these particles may cause
irreversible damage to the electrodes. Use lint-free wipes to remove any residual particles after washing. Put some deionized
water or 70% ethanol on the wipes and gently wipe the interior and surfaces of the ETC Chamber. Do not use excessive amounts
of liquid. Do not allow liquid to enter the device as this may cause electric damage. Dry the ETC Chamber thoroughly.
5.2 Snap-Lock Connector Cleaning
Clean the snap-lock connector after each run. The Electrophoretic Tissue Clearing Solution will crystallize in the crevices, which
may lead to the chamber outlet and the snap-lock connector not connecting properly. Press and release the stainless steel
button on the side of the snap-lock connector repeatedly to shake off any crystals that may have formed. Use lint-free wipes
dampened with water to wipe the snap-lock connector head. Take special care to clean the parts indicated below. Dry the snap-
lock connector thoroughly.

15
5.3 Troubleshooting
Symptom
Possible Cause
Solution
Tissue is not clear
Inappropriate voltage and/or current applied
Check the voltage and current settings.
Low temperature
Set the temperature to 37-50°C .
Short clearing time
Run the system for longer.
Inappropriate clearing solution
Use fresh Electrophoretic Tissue Clearing
Solution.
Incorrect pH
Use fresh Electrophoretic Tissue Clearing
Solution (do not adjust the pH).
Insufficient polymerization of hydrogel
Check if the ingredients of the hydrogel
solution were fresh. Perform hydrogel
polymerization in an oxygen-free
environment.
Dark spots on the tissue
Dirty ETC Chamber
Clean the ETC Chamber thoroughly prior to
use. See Section 5.1: Cleaning the ETC
Chamber.
Presence of items such as staples, tape, etc.
Only place tissues and authorized containers
and container holders in the ETC Chamber.
Tissue is yellow
Reused clearing solution
Change the Electrophoretic Tissue Clearing
Solution with a cycle of 1.2 L/day for a whole
adult mouse brain.
More than one adult mouse brain per
chamber
Reused tissue container
Use a new tissue container.
Tissue is deformed
Over-clearing
Reduce the time or current for the ETC
process.
Displayed and set
temperature are
different
It is not unusual for the displayed
temperature to go above the set value.
Contact your local distributor or Logos
Biosystems when the difference is ≥10°C .
High temperatures in the
tube attached to the
chamber outlet
Convection
Convection is a normal phenomenon. The
temperature of the clearing solution in the
tube will be ~5°C higher than indicated on
the display.
Leaking from the
chamber cap
Improperly or loosely screwed on chamber
cap
Screw on the chamber cap tightly.
Aging of or damage to the silicone ring
Replace the chamber cap. Contact your local
distributor or Logos Biosystems.
Voltage and current
values are 0 despite the
settings
Opened ETC chamber safety cover
Close the safety cover.
RUN/STOP button not pushed
Push the RUN/STOP button. A red halo
around the button means the device is on.
The ETC Controller may be malfunctioning.
Contact your local distributor or Logos
Biosystems.

16
5.4 Error Codes
Error code
Possible Cause
Solution
E01
Malfunctioning part
Reboot your system. If the error code persists, contact your local
distributor or Logos Biosystems.
E02
Overheating due to high environmental
temperature, a malfunctioning controller,
or a malfunctioning cooling system
Set up your system in a cooler environmental temperature (less
than 35°C). If the error code persists, contact your local distributor
or Logos Biosystems.

17
6Ordering Information
The following products can be ordered through your local distributor or by contacting [email protected].
Category
Cat #
Product
Unit
Starter Kit
C10001
X-CLARITY™ Tissue Clearing System
1 unit
Main
Components
C10101
X-CLARITY™ ETC Chamber
1 unit
C10201
X-CLARITY™ ETC Controller
1 unit
C10301
X-CLARITY™ Pump
1 unit
C10401
X-CLARITY™ Reservoir
1 unit
Containers &
Holders
C12001
Tissue Container (20 units)
1 box
C12002
Container Holder for 1 Tissue Container
1 unit
C12004
Mouse Brain Slice Holder (Chamber Height: 4 mm)
1 unit
C12007
Whole Rat Brain Holder
1 unit
Replacements
C12101
Multi-Cable
1 unit
C12102
X-CLARITY™ Reservoir Cap with Temperature Probe
1 unit
C12103
Snap-Lock Connector Tube
1 unit
C12104
Peristaltic Pump Tube
1 unit
C12201
Power Cord
1 unit
Reagents
C13001
Electrophoretic Tissue Clearing Solution
12 x 1 L
C13101
X-CLARITY™ Mounting Solution
1 x 25 mL
C13102
X-CLARITY™ Mounting Solution Value Pack
10 x 25 mL

18
7Purchaser Notification
Limited Use Label License: Research Use Only
The purchaser of this product should use this product only for research for the sole benefit of the purchaser. By use of this
product, the purchaser agrees to be bound by the terms of this limited use statement whether the purchaser is a for-profit or a
not-for-profit entity. If the purchaser is not willing to accept the conditions of this limited use statement and this product is
unused, the Company will accept return of the product with a full refund. The purchaser cannot re-sell or otherwise transfer (a)
this product, (b) its components, or (c) materials made using this product or its components to a third party for Commercial
Purposes. Commercial Purposes mean any and all uses of this product and its components by a party for monetary or other
consideration, including but not limited to, (a) product manufacture, (b) providing a service, information, or data, (c)
therapeutic, diagnostic, or prophylactic purposes, or (d) resale of this product or its components whether or not such product
and its components are resold for use in research.
Logos Biosystems, Inc. (“Company”) will not claim any consideration against the purchaser of infringement of patents owned or
controlled by the Company that cover the product based on the manufacture, use, or sale of a therapeutic, clinical diagnostic,
vaccine, or prophylactic product developed in research by the purchaser in which this product or its components were
employed, provided that neither this product nor any of its components were used in the manufacture of such product.
For any use other than this limited use label license of research use only, please contact the Company.
CLARITY™ is the registered trademark of Stanford University. X-CLARITY™ is the registered trademark of Logos Biosystems, Inc.
Warranty
Logos Biosystems, Inc. (“Company”) warrants to the original purchaser (“Purchaser”) that the system (“System”), if properly used
and installed, will be free from defects in materials and workmanship and will conform to the product specifications for a period
of one (1) year (“Warranty Period”) from the date of purchase. Actual warranty periods may vary depending on customer
location. If the System under this limited warranty fails during the Warranty Period, the Company, at its sole responsibility, will:
1) within and up to 30 calendar days of purchase, refund the purchase price of the System to the Purchaser if the System
is in original conditions; or,
2) after 30 calendar days of purchase, only replace or repair the System for up to the Warranty Period without issuing a
credit.
In no event shall the Company accept any returned System (including its components) that might have been used or
contaminated in some labs, including but not limited to, HIV or other infectious disease or blood-handling labs. This limited
warranty does not cover refund, replacement, and repair incurred by accident, abuse, misuse, neglect, unauthorized repair, or
modification of the System. This limited warranty will be invalid if the System is disassembled or repaired by the Purchaser.
In case that the Company decides to repair the System, not to replace, this limited warranty includes replacement parts and
labor for the System. This limited warranty does not include shipment of the System to and from service location or travel cost
of service engineer, the costs of which shall be borne by the Purchaser. Every effort has been made to ensure that all the
information contained in this document is correct at its publication. However, the Company makes no warranty of any kind
regarding the contents of any publications or documentation as unintended or unexpected errors including occasional
typographies or other kinds are inevitable. In addition, the Company reserves the right to make any changes necessary without
notice as part of ongoing product development. If you discover an error in any of our publications, please report it to your local
supplier or the Company. The Company shall have no responsibility or liability for any special, incidental, indirect or
consequential loss or damage resulting from the use or malfunction of the System.
This limited warranty is sole and exclusive. The Company makes no other representations or warranties of any kind, either
express or implied, including for merchantability or fitness for a particular purpose with regards to this System. To obtain service
during the Warranty Period, contact your local distributor or the Company.
Out of Warranty Service
Please contact your local supplier or the Company’s technical support team in order to obtain out-of-warranty service. If
necessary, repair service will be charged for replacement parts and labor hours incurred to repair the System. In addition, the
Purchaser is responsible for the cost of shipping the System to and from the service facility and, if necessary, the travel cost of a
service engineer.

Logos Biosystems
Headquarters
Doosan Venturedigm Suite 514
415 Heungan-daero, Dongan-gu
Anyang-si, Gyeonggi-do 14059
SOUTH KOREA
Tel: [phone]
Fax: [phone]
USA
7700 Little River Turnpike Suite 207
Annandale, VA 22003
USA
Tel: [phone]
Tel: [phone]
Fax: [phone]
[email protected]om
www.logosbio.com