ThermoFisher Scientific HERASAFE 2025 User manual

Valid: 30.08.2017 / 50155204

Copyright 2019
These operating instructions are protected by copyright. Rights resulting thereof, particularly reprint, photomechanical or
digital postprocessing or reproduction, even in part, are only allowed with the written consent of Thermo Electron LED
GmbH. This regulation does not apply to reproductions for in-plant use.
Trademarks
Thermo Scientific is a brand of Thermo Fisher Scientific. All other trademarks mentioned in the operating instructions are
the exclusive property of the respective manufacturers.
Thermo Electron LED GmbH
Robert-Bosch-Straße 1
D - 63505 Langenselbold
Germany
Thermo Electron LED GmbH is a subsidiary company of:
Thermo Fisher Scientific Inc.
168 Third Avenue
Waltham, MA 02451
USA

Herasafe 2030i / Maxisafe 2030i 50161258 | 3
Table of Contents
Chapter 1 Table of Contents ............................................................................................................................................... 3
Chapter 1 Figures ............................................................................................................................................................... 7
Chapter 1 General Notes..................................................................................................................................................... 9
General Safety Instructions........................................................................................................................... 10
Warranty..................................................................................................................................................... 10
Explanation of Symbols ................................................................................................................................ 11
Symbols used in the Operating Instructions .............................................................................................. 11
Symbols on the Device ............................................................................................................................ 11
Use of the Device......................................................................................................................................... 13
Correct Use ............................................................................................................................................ 13
Incorrect Use .......................................................................................................................................... 13
Standards and Safety Regulations................................................................................................................. 14
Chapter 2 Delivery ............................................................................................................................................................ 15
Items supplied............................................................................................................................................. 15
Acceptance Inspection ................................................................................................................................. 15
Transport Lock and Device Packaging........................................................................................................... 15
Chapter 3 Installation of the Device.................................................................................................................................. 17
Ambient Conditions...................................................................................................................................... 17
Room Ventilation.......................................................................................................................................... 18
Correct Location .......................................................................................................................................... 18
Installation in Series..................................................................................................................................... 18
Connections ........................................................................................................................................... 19
Power Receptacles ................................................................................................................................. 19
Transport .................................................................................................................................................... 20
Chapter 4 Unit Description................................................................................................................................................ 21
Overall View ................................................................................................................................................ 21
Safety System ............................................................................................................................................. 22
Filter System ............................................................................................................................................... 24
Controls and Display .................................................................................................................................... 25
Sample Chamber Access ............................................................................................................................. 26
Device Interfaces ......................................................................................................................................... 28
UV Lamp Unit .............................................................................................................................................. 30
Working Area .............................................................................................................................................. 31
Chapter 5 Start-up............................................................................................................................................................ 33
Initial Operation ........................................................................................................................................... 33
Installing Unit and Accessories ..................................................................................................................... 33
Leveling the Device...................................................................................................................................... 35
Power Supply Connection............................................................................................................................. 36
USB-Port..................................................................................................................................................... 36
Installation Test ........................................................................................................................................... 37

Table of Contents
4 | 50161258 Herasafe 2030i / Maxisafe 2030i
Chapter 6 Operation ......................................................................................................................................................... 39
Display........................................................................................................................................................ 39
Explanation of the Display Components .................................................................................................... 39
Display in OFF-Mode .............................................................................................................................. 40
Display in Work-Mode ............................................................................................................................. 40
Power Interruption .................................................................................................................................. 40
Display and Functions after a Power Failure ............................................................................................. 40
Failure Messages ................................................................................................................................... 40
Description of the Operating Modes.............................................................................................................. 41
Operation.................................................................................................................................................... 43
Moving the Front Window into Working Position ....................................................................................... 44
Audible Warn Signal ............................................................................................................................... 45
Switching the Illumination ON and OFF .................................................................................................... 45
Activating/Deactivating Internal Power Supply ........................................................................................... 45
Changing UV-Desinfection Time .............................................................................................................. 45
Switching the Cabinet to OFF-Mode ......................................................................................................... 46
Setting Time ........................................................................................................................................... 46
Display Operating Hours Air Inflow ........................................................................................................... 47
Display Air Inlet Velocity .......................................................................................................................... 47
Display Downflow Velocity ....................................................................................................................... 47
Setting UV-Disinfection Time ................................................................................................................... 48
Start UV-Disinfection ............................................................................................................................... 49
Cancel UV-Disinfection ............................................................................................................................ 49
Rocker Switch ............................................................................................................................................. 49
Moving the Front Window ........................................................................................................................ 50
Chapter 7 Preparation ...................................................................................................................................................... 51
Hygiene Preparations for the Sample Chamber.............................................................................................. 51
Preparing the Sample Chamber.................................................................................................................... 51
Response to Failure Messages ..................................................................................................................... 52
Work Rules ................................................................................................................................................. 52
Chapter 8 Shut-down ....................................................................................................................................................... 55
Interrupting an Operation ............................................................................................................................. 55
Shutting the Unit down................................................................................................................................. 55
Chapter 9 Cleaning and Decontamination ........................................................................................................................ 57
Decontamination Procedure ......................................................................................................................... 57
Wipe/Spray Disinfection ............................................................................................................................... 57
UV Disinfection after a Wipe/Spray Disinfection.............................................................................................. 59
UV Disinfection using the integral UV Lamps ............................................................................................. 59
Changing the UV Disinfection Time .......................................................................................................... 59
Disinfection with Formaldehyde .................................................................................................................... 59
Cleaning the exterior Surfaces...................................................................................................................... 60
Cleaning the Front Window .......................................................................................................................... 60
Cleaning the Drain Pan ................................................................................................................................ 60
Replacing the inlet air protection................................................................................................................... 61

Herasafe 2030i / Maxisafe 2030i 50161258 | 5
Table of Contents
Chapter 10 Maintenance .................................................................................................................................................. 63
Inspection ................................................................................................................................................... 63
Service ....................................................................................................................................................... 63
UV Lamps .............................................................................................................................................. 63
Sample Chamber Illumination .................................................................................................................. 64
Replacing the Front Cover Seal ................................................................................................................ 64
Retrofitting and Repairs................................................................................................................................ 64
Chapter 11 Disposal ......................................................................................................................................................... 65
Disposal Procedure...................................................................................................................................... 65
Chapter 12 Technical Data ............................................................................................................................................... 67
Chapter 13 Device Log ..................................................................................................................................................... 73
Chapter 14 Certificate of Decontamination ...................................................................................................................... 75

Table of Contents
6 | 50161258 Herasafe 2030i / Maxisafe 2030i

Herasafe 2030i / Maxisafe 2030i 50161258 | 7
Figures
Figure 1 Correct Location.....................................................................................................................................18
Figure 2 Lift Points .............................................................................................................................................20
Figure 3 Overall View, Herasafe 2030i version ......................................................................................................21
Figure 4 Filter System with Downflow Filter and Exhaust Air Filter...........................................................................24
Figure 5 Controls and Display...............................................................................................................................25
Figure 6 Access through Front Cover....................................................................................................................26
Figure 7 Access through Front Window.................................................................................................................27
Figure 8 Supply Interfaces ...................................................................................................................................28
Figure 9 Workspace Illumination...........................................................................................................................29
Figure 10 UV Lamp Unit.......................................................................................................................................30
Figure 11 Working Area on the Workplate, Armrests..............................................................................................31
Figure 12 Stand Installation..................................................................................................................................34
Figure 13 USB-Port .............................................................................................................................................36
Figure 14 Explanation of the Display components..................................................................................................39
Figure 15 Rocker switch ......................................................................................................................................49
Figure 16 Sitting Posture .....................................................................................................................................53
Figure 17 Inlet air protection ................................................................................................................................61

Figures
8 | 50161258 Herasafe 2030i / Maxisafe 2030i

Herasafe 2025 50161258 | 9
General Notes
The following are the addresses of the international Thermo Sales Organisations.
Postal address Germany
Thermo Electron LED GmbH
Robert-Bosch-Straße 1
D - 63505 Langenselbold
Enquiries from Germany
Phone
Sales 0800 1 536376
Service 0800 1 112110
Fax
Sales/Service 0800 1 112114
E-Mail
[e-mail]
Enquiries from Europe, Middle East and Africa
Tel. + 49 (0) 6184 / 90-6940
Fax + 49 (0) 6184 / 90-7474
Enquiries from North America:
Phone [phone] [phone]
Fax [phone]
Enquiries from Latin America:
Phone [phone]
Fax [phone]
Enquiries from Asia Pacific:
Phone [phone]
Fax [phone]
E-Mail
[e-mail]

| General Notes
10 | 50161258 Herasafe 2025
Chapter 1
General Safety Instructions
These safety instructions describe the safety features of the Herasafe 2025 series.
The safety cabinet has been manufactured in keeping with the latest technological
developments and has been tested before delivery for its correct function. It may, however,
present potential hazards if it is not used according to the intended purpose or outside of
operating parameters. Therefore, the following procedures must always be observed:
The safety cabinet must be operated only by trained and authorized personnel.
For any operation of this unit, the operator must prepare clear and concise written
instructions in the language of the operating and cleaning personnel based on these
operating instructions, applicable safety data sheets, plant hygiene guidelines, and
technical regulations, in particular:
which decontamination measures are to be applied for the cabinet and accessories,
which protective measures apply while specific agents are used,
which measures are to be taken in the case of an accident.
Repairs to the device must be carried out only by trained and authorized expert personnel.
The contents of the operating instructions are subject to change without further notice.
Concerning translations into foreign languages, the German version of these
operating instructions is binding lease.
Keep these operating instructions close to the unit so that safety instructions and
important information are always accessible.
Should you encounter problems that are not detailed adequately in these operating
instructions, please contact Thermo Electron LED immediately for your own safety.
Warranty
Thermo Electron LED warrant the operational safety and functions of the safety cabinet
only under the condition that:
the device is operated and serviced exclusively in accordance with its intended
purpose and as described in these operating instructions,
the device is not modified,
only original spare parts and accessories that have been approved by Thermo
Electron LED are used,
inspections and maintenance are performed at the specified intervals,
an installation test is performed prior to the initial operation of the device and that a
repeat test is performed on the occasion of all inspections and repairs.
The warranty is valid from the date of delivery of the device to the operator.
Impairment of the system may lead to hazards.

Herasafe 2025 50161258 | 11
General Notes | Chapter 1
Explanation of Symbols
Symbols used in the Operating Instructions
Symbols on the Device
Is used if non-observance may cause serious or even lethal injuries.
Is used if non-observance may cause medium to minor injuries or damage.
NOTE
is used for hints and useful information.
Valuable raw materials can be reused.
Observe operating instructions
Biohazard
USB interface
CE conformity mark: confirms conformity according to EU Directives
GS sign TÜV Nord

| General Notes
12 | 50161258 Herasafe 2025
Chapter 1
Filter replacement
2 x T5A outlet fusing (at mainboard)
2 x T 15 AH (at mainboard)
2 x T 16 AH Equipment Fusing (inside the electronic module)
X20 connection for an external fan
X21 connection for a solenoid valve
X22 connection for an external
component
Armrest installation
UV lamp
At the supply lines for measurement of the raw air
concentration by leakage testing of the filter.

Herasafe 2025 50161258 | 13
General Notes | Chapter 1
Use of the Device
Correct Use
Herasafe 2025:
The safety cabinet is a laboratory device for installation and operation in microbiological
and biotechnical laboratories of safety levels 1, 2, and 3. It has been designed as a Class
II microbiological safety cabinet, in accordance with EN 12469:2000.
Depending on the hazard level of the agents involved, the operator must prepare in writing
appropriate decontamination procedures for the device and the accessories used in the
sample chamber.
Following media should be used:
Vacuum (Rough vacuum 10 E-2 bar / 0,145 psi)
Combustible gas (e.g. Propane)
Non-combustible gas (e.g. Nitrogen)
Incorrect Use
The safety cabinet must not be used in laboratories that do not comply with the
requirements of safety levels 1, 2, and 3.
The unit must not be operated as a Class II safety cabinet if:
no repeat test is performed after changes to the installation conditions or after
modifications to the technical system.
the alarm system of the device has issued a failure message and the cause for the
failure has not been repaired.
The alarm system must not be tampered with or disabled. If alarm system components
have been removed or disabled for service or repairs, the unit must only be released for
operation if all alarm system components are functioning again properly.
The filters installed in the device are not capable of separating gaseous substances.
Therefore, do not work with or store substances in the device:
are toxic which in quantity or concentration,
if a reaction with other substances may result in hazardous toxic concentrations or
formation of toxic gases,
that may form combustible or explosive mixtures in combination with air.

| General Notes
14 | 50161258 Herasafe 2025
Chapter 1
Standards and Safety Regulations
The device complies with the safety requirements of the following standards and guidelines:
IEC 61010-1:2010 / EN 61010-1:2010
EN 12469:2000
EN 61326-1:2018
Low Voltage Directive 2014/35/EU
EMC Directive 2014/30/EU
RoHS directive 2011/65/EU
WEEE directive 2012/19/EU
China EEP Hazardous Substances Information
http://www.thermofisher.com/us/en/home/technical-resources/rohs-certificates.html

HERAsafe 2025 50155215 | 15
Delivery
Items supplied
Delivery for the safety cabinet includes the following:
Herasafe 2025 version:
safety cabinet
armrests
device documentation with:
operating instructions (CD)
factory test report
summarized safety instructions (SSI)
HEPA filter certificate
Optional components and accessories are listed as separate items in the delivery document.
Acceptance Inspection
After the device has been delivered, immediately check the device:
for completeness,
for possible damage.
If the delivery is incomplete or if you detect any transport damage to the device,
contact the forwarding agency and Thermo Electron LED immediately.
Transport Lock and Device Packaging
Do not transport the device over large distances without transport lock and original device
packaging.

| Delivery
16 | 50155215 HERAsafe 2025
Chapter 2

Herasafe 2025 50161258 | 17
Installation of the Device
Ambient Conditions
The operational safety and correct function of the unit depend on the location where it is
to be operated. The safety cabinet must be operated only at locations that meet the
ambient conditions listed below.
Location requirements:
The electrical system of the device has been designed for an operating height of up
to 2.000 m above sea level.
The mains power supply outlets should be out of casual reach to prevent accidental
shut-off. Ideally, the outlets should be installed above the safety cabinet and be
readily accessible.
The flooring of the location must be adequately strong and not flammable.
The support frame must be designed with an adequate load-carrying capacity
(supporting four times the weight of the device).
The room in which the device is installed must be of adequate height. For units not
connected to an exhaust system, the distance between the exhaust air opening and
the room ceiling must be at least 200 mm. There is potential to install with a lower
clearance of 200mm. This should be discussed with a Thermo Fisher Scientific BSC
specialist.
The location must be equipped with an appropriate ventilation system, see “Room
Ventilation” on page 18.
The temperature within the room must be between 10 °C and 40 °C.
The power supply voltage should not vary by ±10 % from its nominal value.
Transient overvoltage peaks shall not exceed the usual range prevailing within the AC
power supply grid. The nominal transient overvoltage level shall be the surge
withstand voltage according to overvoltage category II of IEC60364-4-443.
An appropriate ground fault circuit interrupter must be installed by the customer to
protect the device system.
Condensation must be avoided, for example, subsequent to relocation or transport.
Should condensation exist, wait for the humidity to evaporate completely before
connecting to a power source or powering up the device.
The relative humidity in the vicinity of the device must not exceed 90 %.
NOTE Ambient conditions
If ambient conditions vary from those described above, please contact Thermo
Electron LED for assistance in installing the device.
NOTE Temporary storage
If the device is stored only temporarily (up to four weeks), the ambient
temperature may be between -20 °C and +60 °C (-4 °F and +140 °F) at a
relative air humidity of up to 90 %. For longer storage periods, the location
requirements apply.

| Installation of the Device
18 | 50161258 Herasafe 2025
Chapter 3
Room Ventilation
The room ventilation should preferably be a ventilation system that complies with the
national requirements for the application.
The inlet air and exhaust air openings of the room ventilation must be located so that
drafts are prevented from impairing the function of the safety cabinet air system.
Correct Location
Choose a draft-free location where the safety cabinet does not interfere with the plant traffic.
Figure 1 Correct Location
This figure shows preferred locations for safety cabinets and unsuitable locations, not in
accordance with the safety requirements.
Unsuitable locations: The locations [1], [2] and [3] are not suitable because they are
exposed to drafts from windows and doors.
Location [5] is undesirable because it is in range of plant traffic and within the exhaust air
range of a ventilation system [4].
Preferred locations: Preferred locations [6], [7], and [8] are correct because they are in
a draft-free section of the room and not exposed to plant traffic.
Installation in Series
When several devices are to be installed in series, please observe the following:
Make sure that vibrations cannot be transferred between adjacent units,
exterior surfaces of the cabinets must always be accessible for cleaning and disinfection,
minimum distance of 2 m (6.6 ft.) between workbenches on opposite position.

Herasafe 2025 50161258 | 19
Installation of the Device | Chapter 3
Connections
Cable glands allow for connecting up to 3 external devices.
Connect directly to the printed circuit board!
Caution! Make sure the max. current output is not exceeded!
X22 230V Power Supply / X21 Monitor Alarm / X20 External Fan
The leads can be attached on the outer face of the anti-tilt anchor using cable ties.
Power Receptacles
The power receptacles are fused using a T 5A slow-blow fuse.
Power cable
X22 X21 X20
High voltage
Before starting any work on electrical and electronic components, the unit must
be disconnected from the power supply. The work may only be carried out by an
electrically qualified person.

| Installation of the Device
20 | 50161258 Herasafe 2025
Chapter 3
Transport
Herasafe 2025:
Figure 2 Lift Points
To prevent tilting, always transport the device using a suitable carrier, even for a transport
within a building, and separate it from the stand.
Danger of tipping over!
For transport, lift the device only using the lift points shown in the illustration.
Do not load the drain pan with the weight of the device frame!
Contusion hazard!
When lifting the safety cabinet, do not put hands or fingers between drain pan
and frame!