Biotix xPipette User manual

2 xPIPETTE™
Table of Contents Introduction
Introduction
Parts Checklist ........................................ 3
GLP Compliance ..................................... 3
Description .............................................. 3
Specications .......................................... 4
Setting Volume
Instructions .............................................. 6
Pipetting
Tip Selection ............................................ 8
Tip Mounting ............................................ 8
Operation ................................................. 8
Dispensing ............................................... 8
Good Pipetting Guidelines ....................... 9
Pre-rinsing ............................................... 9
Pipetting Liquids of Varying Densities ...... 9
Temperature Considerations ...................... 9
Reverse Pipetting .................................. 10
Troubleshooting
Troubleshooting and Repairs ................. 11
Sample Splash ...................................... 12
Leaks, Inaccuracy, Abnormal Stroke ..... 12
Service, Calibration and Repair ............. 12
Maintenance
Autoclaving ............................................ 13
Tip Ejector Arm Removal ....................... 13
Storage .................................................. 13
Acids and Corrosives ............................. 13
Parts ....................................................... 13
Accessories
Pipette Carousel .................................... 14
Replacement Parts ................................ 14
Warranty
3
6
8
11
13
14
14

3 www.biotix.com
Table of Contents Introduction
3
6
8
11
13
14
14
xPIPETTE is an air-displacement pipette that incorporates
major ergonomic improvements to minimize strain and
help reduce the risk of repetitive stress injuries. Low spring
forces and low force seals contribute to a lighter feel. A
magnet is used to help balance and hold the piston in the
zero position, which reduces static force on the operator’s
hand during the pipetting cycle.
Parts Checklist
After unpackaging, please verify that the following items
were included and are undamaged:
• xPIPETTE
• xPIPETTE User Guide
• Calibration Certicate
GLP Compliance
The unique pipette serial number is
engraved on the body of the pipette
underneath the handle. It provides
a unique identication to to help
fulll GLP requirements.
Calibration certicates are also
included for further traceability.
Description
Connecting Nut
Shaft
Stainless Steel
Tip Ejector Arm
Digital Volume Indicator
Plunger
Body:
high-impact, low thermal
conductivity polymer
Handle
Volume Lock
Tip Ejector
2 μL
1
2
5
1.25 μL
10 μL
0
7
5
7.5 μL
20 μL
1
2
5
12.5 μL
100 μL
0
7
5
75 μL
200 μL
1
2
5
125 μL
300 μL
2
2
5
225 μL
1000 μL
0
7
5
0.75 mL
0
6
7
1
0
8
1
5
67 μL 108 μL 157.6 μL
xPIPETTE Manual

4 xPIPETTE™
Specications
Single Channel Pipettes
BIOTIX ISO 8655
Volume* Systematic
Error
Random
Error
Systematic
Error
Random
Error
2 μL with xTIP4 20 μL tips (P/N 63300160)
0.2 ± 0.024 ≤ 0.012 ± 0.08 ≤ 0.04
1± 0.027 ≤ 0.013 ± 0.08 ≤ 0.04
2± 0.03 ≤ 0.014 ± 0.08 ≤ 0.04
10 μL with xTIP4 20 μL tips (P/N 63300161)
1± 0.025 ≤ 0.012 ± 0.12 ≤ 0.08
5 ± 0.075 ≤ 0.03 ± 0.12 ≤ 0.08
10 ± 0.1 ≤ 0.04 ± 0.12 ≤ 0.05
20 μL with xTIP4 20 μL tips (P/N 63300162)
2± 0.15 ≤ 0.04 ± 0.20 ≤ 0.10
10 ± 0.15 ≤ 0.05 ± 0.20 ≤ 0.10
20 ± 0.2 ≤ 0.06 ± 0.20 ≤ 0.10
100 μL with xTIP4 200 μL tips (P/N 63300163)
10 ± 0.35 ≤ 0.10 ± 0.80 ≤ 0.30
50 ± 0.4 ≤ 0.12 ± 0.80 ≤ 0.30
100 ± 0.8 ≤ 0.15 ± 0.80 ≤ 0.30
200 μL with xTIP4 200 μL tips (P/N 63300164)
20 ± 0.5 ≤ 0.20 ± 1.60 ≤ 0.60
100 ± 0.8 ≤ 0.25 ± 1.60 ≤ 0.60
200 ± 1.6 ≤ 0.30 ± 1.60 ≤ 0.60
300μL with xTIP4 300 μL tips (P/N 63300327)
30 ± 0.75 ≤ 0.30 ± 2.40 ≤ 0.90
150 ± 1.2 ≤ 0.375 ± 2.40 ≤ 0.90
300 ± 2.4 ≤ 0.45 ± 2.40 ≤ 0.90
1000μL with xTIP4 1000 μL tips (P/N 63300165)
100 ± 3 ≤ 0.6 ± 8.0 ≤ 3.0
500 ± 4 ≤ 1 ± 8.0 ≤ 3.0
1000 ± 8 ≤ 1.5 ± 8.0 ≤ 3.0
These manufacturer’s specications should be used as
guidelines when establishing your own performance
specication.

5 www.biotix.com
Specications
Multi-Channel Pipettes
BIOTIX ISO 8655
Volume* Systematic
Error
Random
Error
Systematic
Error
Random
Error
10 μL with xTIP4 20 μL tips
(P/N 63305170 & 63305174)
1± 0.040 ≤ 0.05 ± 0.08 ≤ 0.10
5 ± 0.075 ≤ 0.075 ± 0.15 ≤ 0.15
10 ± 0.100 ≤ 0.05 ± 0.20 ≤ 0.10
20 μL with xTIP4 20 μL tips
(P/N 63305171 & 63305175)
1 ± 0.15 ≤ 0.08 ± 0.30 ≤ 0.16
5± 0.15 ≤ 0.15 ± 0.30 ≤ 0.30
10 ± 0.20 ≤ 0.10 ± 0.40 ≤ 0.20
200 μL with xTIP4 200 μL tips
(P/N 63305172 & 63305176)
2 ± 0.50 ≤ 0.24 ± 1.00 ≤ 0.48
10 ± 0.80 ≤ 0.25 ± 1.60 ≤ 0.50
20 ± 1.60 ≤ 0.60 ± 3.20 ≤ 1.20
300 μL with xTIP4 300 μL tips
(P/N 63305173 & 63305177)
10 ± 0.75 ≤ 0.10 ± 1.50 ≤ 0.20
50 ± 1.20 ≤ 0.12 ± 2.40 ≤ 0.30
100 ± 2.40 ≤ 0.15 ± 4.80 ≤ 0.30
*Specications are subject to change without notice
* All volumes are in microliters
xPIPETTE Manual

6 xPIPETTE™
Setting the Volume
Unlocked Locked
Volume is locked when
wings are straight across
Wings
Instructions
1. Turn the volume lock counter-clockwise to the position
shown at left below so the volume setting mechanism is
unlocked and free to turn.
2. With the mechanism unlocked, orient the pipette so you
are looking at the digital volume indicator, then rotate the
plunger button to change volume – counter-clockwise to
increase, clockwise to decrease volume.
The volume indicator is read from the top down and is
color-coded in relation to where the decimal point should
go.
2-20 μL: Black - μL.
Red- tenths, hundredths of μL.
100 -300 μL: All digits black - whole μL.
1000 μL: Red - mL. Black - tenths, hundreds of mL.
3. To eliminate errors due to mechanical backlash: when
setting the desired volume, rst turn the knob 1⁄3 turn
above the desired volume. Then turn the knob slowly
clockwise until the desired volume is displayed.
4. Turn the volume lock clockwise (see diagram above) to
prevent any accidental changes to the volume setting.
Example volumes are shown on the next page (note the
intermediate setting at the right).
2 μL
1
2
5
1.25 μL
10 μL
0
7
5
7.5 μL
20 μL
1
2
5
12.5 μL
100 μL
0
7
5
75 μL
200 μL
1
2
5
125 μL
300 μL
2
2
5
225 μL
1000 μL
0
7
5
0.75 mL
0
6
7
1
0
8
1
5
67 μL 108 μL 157.6 μL
2 μL
1
2
5
1.25 μL
10 μL
0
7
5
7.5 μL
20 μL
1
2
5
12.5 μL
100 μL
0
7
5
75 μL
200 μL
1
2
5
125 μL
300 μL
2
2
5
225 μL
1000 μL
0
7
5
0.75 mL
0
6
7
1
0
8
1
5
67 μL 108 μL 157.6 μL

7 www.biotix.com
Setting the Volume
Volume ranges and increments for single-channel pipettes
are shown below:
Volume ranges and increments for multi-channel pipettes are
shown below:
Pipette Size
(μL)
Adjustable
Range
(μL)
Recommended
Volume Range
(μL)
Increment
(μL)
2 0 - 2 0.1 - 2 0.002
10 0 - 10 0.5 - 10 0.02
20 0 - 20 2 - 20 0.02
100 0 - 100 10 - 100 0.2
200 0- 200 20 - 200 0.2
300 0 - 300 30 - 300 0.5
1000 0 - 1000 100 - 1000 2.0
Pipette Size
(μL)
Adjustable
Range
(μL)
Recommended
Volume Range
(μL)
Increment
(μL)
10 0 - 10 0.5 - 10 0.02
20 0 - 20 2 - 20 0.02
200 0- 200 20 - 200 0.02
300 0 - 300 30 - 300 0.5
xPIPETTE Manual

8 xPIPETTE™
Pipetting
Tip Selection
To ensure top performance of your xPIPETTE, we recom-
mend that you use Biotix xTIP4 pipette tips. Together, the
pipette-tip combination creates an ergonomic system to
reduce insertion and ejection forces. The xPIPETTE is also
fully compatible with Rainin®LTS™tips.
Tip Mounting
Single-Channel Pipette: To mount a tip, press the pipette
shaft nozzle into the end of the tip with light force.
Multi-Channel Pipette: Align the shaft nozzles into the row
of tips, holding the pipette at an angle. Position the pipette
upright and press the nozzles into the tips until securely
mounted.
Operation
Before pipetting valuable samples, we recommend that you
practice aspirating and dispensing water before pipetting
with actual samples.
1. Set the desired volume as described on page 7.
2. Attach a new tip. Press the shaft into the tip to make a
good seal.
3. Press the plunger button to the FIRST STOP, and hold it in
this position. The magnetic assist will help you sense and
hold this position.
4. Holding the pipette vertically, place the tip into the sam-
ple to the proper depth and relax your thumb pressure
on the plunger. The light piston spring will move the
piston upward, aspirating your sample. Do not let go of
the plunger button suddenly, or the piston may snap up
quickly, resulting in an inaccurate measurement.
5. Pause briey to ensure that the full volume of the sample
is drawn into the tip.
6. Withdraw the tip from the sample. If any liquid remains
on the outside of the tip, wipe it carefully with a lint-free
tissue, taking care not to touch the tip orice.
Dispensing:
1. Touch the tip end against the sidewall of the receiving
vessel and press the plunger slowly, past the rst stop, to
blowout (bottom of stroke).
First Stop
Blowout
Rest Position
Step 1 Step 2

9 www.biotix.com
Pipetting
Wait: 1-2 seconds for 2–300 μL volumes, 2-3 seconds for
1000 μL (longer for viscous solutions).
2. Still holding the plunger, withdraw the tip, sliding it along
the wall of the vessel. Release the plunger.
3. Press the tip ejector button to discard the tip. Use a new
tip for each sample to prevent carry-over. Repeat for the
next pipetting cycle.
Good Pipetting Guidelines
xPIPETTE incorporates features to deliver pipetting
consistency. You should also maintain:
1. Consistent pickup and dispense rhythm.
2. Consistent speed and smoothness when pipetting.
3. Consistent pressure on the plunger button at the rst
stop.
4. Consistent immersion depth.
5. Pipette vertically, or within 20° of vertical.
6. Don’t invert or lay the pipette at with liquid in the tip.
Pre-Rinsing Recommended
Some solutions may leave a lm on the inside tip wall. This
lm remains relatively constant in successive pipetting with
the same tip, so excellent precision can be obtained by
relling the tip and using the relled volume as the sample.
Successive samples from this same tip will exhibit good
reproducibility.
Temperature Considerations
Warm or cold liquids can be measured with good preci-
sion by using a consistent pipetting rhythm. This will help
minimize any dierences in heating or cooling eects within
the pipette. Use a new pipette tip each time for optimal
accuracy and precision when measuring samples with
temperatures greatly dierent from ambient, and do not
pre-rinse. As with any air-displacement pipette, you will get
the best results if there is no delay between aspirating the
sample and dispensing it.
Volume Range (μL) Immersion Depth
(mm)
Wait Time
(sec)
0.1-10 1-2 1
10-200 2-3 1
200-1000 3-6 2-3
xPIPETTE Manual

1 0 xPIPETTE™
Pipetting Liquids of Varying Density
xPIPETTE adjustable pipettes allow you to compensate for
solutions with densities that are dierent than water, by
setting the volume slightly higher or lower. The compensa-
tion amount must be determined empirically. For example,
if pipetting 10 μL of CsCl solution, you determine that the
volume delivered is actually 8.5 μL. Change the volume set-
ting to 11.8 μL and repeat the measurements. If the volumes
delivered are still not close enough to 10 μL, make another
slight volume adjustment until the measurements are as
desired.
Reverse Pipetting
Another technique for reducing error due to lm retention,
especially
useful for more viscous liquids, is reverse pipetting. The
operating sequence is reversed:
1. Mount a disposable tip on the pipette shaft nozzle.
2. Press the plunger button fully to the SECOND STOP.
3. Immerse the tip in liquid and allow the button to return
slowly to the resting position. Wait a moment for the liquid
column in the tip to reach equilibrium.
4. Wipe any excess liquid from the outside of the tip without
touching the orice.
5. To dispense, rest the end of the tip against the vessel wall
and press the plunger to the rst stop. Hold this position
for a few seconds, or long enough for the liquid column to
reach equilibrium again.
6. Remove the tip from the receiving vessel without blowing
out the remaining liquid.
7. Return excess sample in the tip to the original sample
container, if desired. Discard the used tip.
Troubleshooting
First Stop
Blowout
Rest Position
Step 2 Step 3 Step 5 Step 7

1 1 www.biotix.com
Troubleshooting and Repairs
Symptom Possible Cause Action
Pipette is
leaking
Loose shaft Tighten the connecting nut
by hand.
Split or cracked shaft Replace shaft. Check to see
if the piston is bent. If bent,
send the instrument for
service (pg. 12).
Worn seal and/or o-ring Examine seal and o-ring.
Replace them as necessary.
Pipette does
not aspirate
properly
Contamination inside
instrument
Clean with distilled water
or isopropyl alcohol. Dry
with lint-free tissue and
reassemble.
Corrosion and/or
staining
Do not use instrument. Re-
turn instrument to supplier.
Pipette is
inaccurate
Improper repair or
assembly
See page 12.
Loose connecting nut Tighten the connecting nut.
Pipette is
not precise
Loose connecting nut Tighten the connecting nut.
Incorrect operator
technique
Operator training.
Damaged piston Return pipette to supplier.
Damaged shaft Replace the shaft.
Worn O-ring or seal Replace both parts.
Tips Fall O
Low-quality tips Use xTIP4 from Biotix.
Damaged shaft Replace shaft.
Damaged tip ejector
causing misalignment
Replace tip ejector.
WARNING
When removing the shaft from the pipette
body, make sure the spring, seal, and
o-ring do not fall o the piston, especially
on the smaller models.
Troubleshooting
xPIPETTE Manual

1 2 xPIPETTE™
Sample Splash (liquid inside the mechanism)
1. Remove the tip ejector arm. Instructions on how to re-
move the ejector are on page 13.
2. On pipettes up to 1000 μL, unscrew the connecting nut
(D) and remove the shaft (E).
3. Inspect the seal assembly and piston for contamination.
The piston should be shiny and free of corrosion. Clean
with distilled water or isopropyl alcohol. Dry with a lint-
free tissue and reassemble after inspecting the interior of
the shaft for any contamination.
4. If piston corrosion or staining is evident, do not use the
instrument.
Leaks, Inaccuracy, Abnormal Stroke
1. Loose shaft. Tighten connecting nut by hand.
2. Split or cracked shaft. Remove the tip ejector and in-
spect the shaft. Replace the shaft if necessary. If the shaft
was dropped, remove it to see if the piston is bent. If so,
send the instrument for service.
3. Worn seal and/or o-ring. All models up to 1000 μL
incorporate a polyethelyne seal and o-ring. Examine the
seal and o-ring, replacing them as necessary. Pull o the
old seal and o-ring, position the new seal and o-ring on
the piston assembly as shown in the drawings on page
13, and reassemble the pipette.
4. Forming the seal. xPIPETTE uses a small amount of
specialized high-quality grease, applied to the o-ring to
make the seal.
Service, Calibration and Repair
Mettler-Toledo is the preferred provider for the calibration
and service of these pipettes. To contact your local service
center please visit:
www.mt.com/service
Specications
These manufacturer’s specications should be used as
guidelines when establishing your own performance application.
Autoclaving
The shaft and tip ejector of the pipette can be autoclaved at
the following settings: 121, 1 bar, 15-20 minutes
Do not autoclave the complete pipette or any parts other
than the shaft and the tip ejector.
NOTE It is not necessary to recalibrate pipette
after changing seals.
Maintenance

1 3 www.biotix.com
Maintenance
The multi-channel pipettes are not autoclavable.
Tip Ejector Arm Removal
The tip ejector arm can be removed
with minimum eort - do not use force.
For all single-channel models push the
ejector button and it will expose quick-
release tabs below the handle. Press the
tabs towards the shaft to remove
the ejector.
Storage
After use, store the pipette in a clean safe place. xPIPETTE is
a precision instrument and should be treated with the level
of care appropriate for laboratory instrumentation. Pipettes
should be stored vertically using a hanger or stand when
not in use. The Biotix carousal part number is located in the
accessories section on page 14.
Acids and Corrosives
After pipetting concentrated acids or highly corrosive
solutions, disassemble xPIPETTE. Inspect and clean the
piston assembly, shaft, and seal with distilled water. Dry all
components thoroughly and reassemble. Extensive contact
with corrosive fumes may result in premature seal wear and
damage to the piston. Exposure of internal components to
corrosive fumes can be reduced by using tips with
aerosol barrier lters.
Parts
20 μL 200 μL 1000 μL
A A A
B
B
B
C
C
C
Liquid End
Ejector Arm
A: Piston Assembly
B: Seal Retainer
C:Seal
20 μL 200 μL 1000 μL
A A A
B
B
B
C
C
C
Liquid End
Ejector Arm
A: Piston Assembly
B: Seal Retainer
C:Seal
20 μL 200 μL 1000 μL
A A A
B
B
B
C
C
C
Liquid End
Ejector Arm
A: Piston Assembly
B: Seal Retainer
C:Seal
20 μL 200 μL 1000 μL
A A A
B
B
B
C
C
C
Liquid End
Ejector Arm
A: Piston Assembly
B: Seal Retainer
C:Seal
20 μL 200 μL 1000 μL
A A A
B
B
B
C
C
C
Liquid End
Ejector Arm
A: Piston Assembly
B: Seal Retainer
C:Seal
20 μL 200 μL 1000 μL
A A A
B
B
B
C
C
C
Liquid End
Ejector Arm
A: Piston Assembly
B: Seal Retainer
C:Seal
20 μL 200 μL 1000 μL
A A A
B
B
B
C
C
C
Liquid End
Ejector Arm
A: Piston Assembly
B: Seal Retainer
C:Seal
Push
A: Piston Assembly
B: Seal Retainer
C: Seal (o-ring)
D: Connecting Nut
E: Shaft
F: Ejector Arm
EF
13 www.biotix.com
D
xPIPETTE Manual

1 4 xPIPETTE™
Limited Warranty
See the Limited Warranty and Limitations of Liability Statement.
Specied performance is guaranteed only when xTIP4 tips are used.
Subject to technical changes and to the availability of the accessories supplied
with the instruments.
Part Number
63300880
Rotating Carousel Stand,
holds up to 7 pipettes
Part Number Description
30539600 2μL Shaft
30539517 10μL Shaft
30539599 20μL Shaft
30539516 100μL Shaft
30539598 200μL Shaft
30539601 300μL Shaft
30539515 1000μL Shaft
17001797 Ejector Arm 20μL
17001799 Ejector Arm 200μL
17007556 Ejector Arm 300μL
17001793 Ejector Arm 1000μL
17014318 O-Ring .010 ID 2μL (C, pg 13)
17014689 O-Ring .010 ID 10μL (C, pg 13)
17014320 O-Ring .010 ID 20μL (C, pg 13)
17014289 Seal Lip 200μL (C, pg 13)
17014290 Seal Lip 300μL (C, pg 13)
17014195 Seal Lip 1000μL (C, pg 13)
Accessories
Pipette Carousel:

©2022, Biotix, Inc. All rights reserved.
Biotix, uTIP, xPIPETTE, xTIP4, FlexFit, StarStop, X-Resin,
Blade, Tip Eject, and CleanPak are trademarks of Biotix inc.
All other brands and names contained are the property of
their respective owners as found at biotix.com/trademarks
BIOTIX.BIOTIX.comcom