Thermo Scientific Cryofuge 16 User manual

Thermo Scientific
Cryofuge 16
Heavy Duty Water Cooled Centrifuge
Instruction Manual
50152622-h • 03 / 2021
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WEEE Compliance
This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment
(WEEE) Directive 2012/19/EU. It is marked with the following symbol:
2

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Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges
Table of Contents
Preface .................................................vii
Items Supplied ...................................................vii
Intended Use for Cryofuge 16 Heavy Duty Water Cooled..................viii
Intended User....................................................................................................................... viii
Associated Rotors and Components ...................................................................................... viii
Precautions.......................................................ix
Set Up Conditions...................................................................................................................ix
Preparation .............................................................................................................................x
Hazardous Substances.............................................................................................................x
Operating...............................................................................................................................xi
Maintenance ..........................................................................................................................xi
Symbols used on the Centrifuge .....................................xii
Symbols used in the Manual ........................................xiii
Transport and Set Up .....................................I–1
1. Location ......................................................I–1
2. Transporting...................................................I–3
3. Storage.......................................................I–3
4. Shipping......................................................I–3
Thermo Scientific
Centri-Touch User Interface............................... II–1
Status .........................................................II–2
Run Parameters .................................................II–3
Control and Configuration .........................................II–3
Lighthouse Mode ................................................II–4
Operation .............................................III–1
1. Switch on the Centrifuge .......................................III–1
2. Open the Centrifuge Door .......................................III–1
3. Install a Rotor ................................................III–2
3. 1. Before Installation.................................................................................................III–2
3. 2. Rotor Installation ..................................................................................................III–2

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Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges
4. Maximum Loading.............................................III–8
Use of Tubes and Consumables...........................................................................................III–9
5. Close the Centrifuge Door.......................................III–9
6. Entering Parameters ...........................................III–9
6. 1. Acceleration / Deceleration Profiles .......................................................................III–9
6. 2. Preselect Speed / RCF-Value ................................................................................III–9
6. 3. Runtime Preselection..........................................................................................III–10
6. 4. Preselect Temperature........................................................................................III–10
6. 5. Pre-warm or Pre-cool the Centrifuge...................................................................III–10
7. Programs .................................................. III–11
8. Centrifugation .............................................. III–12
8. 1. Start ..................................................................................................................III–12
8. 2. Stop ..................................................................................................................III–12
9. Remove a Rotor ............................................. III–13
10. Switch off the Centrifuge .................................... III–13
Maintenance and Care ...................................IV–1
1. Cleaning Intervals ............................................ IV–1
2. Basics...................................................... IV–1
2. 1. Rotor and Components Inspection........................................................................ IV–2
3. Cleaning .................................................... IV–3
3. 1. Touchscreen ....................................................................................................... IV–4
3. 2. Filter Mat............................................................................................................ IV–5
4. Disinfection ................................................. IV–6
5. Decontamination ............................................. IV–6
6. Autoclaving ................................................. IV–7
7. Maintenance ................................................ IV–8
7. 1. Preventive Maintenance....................................................................................... IV–8
7. 2. Service ............................................................................................................... IV–8
8. Shipping and Disposal ........................................ IV–9
Troubleshooting .........................................V-1
1. Mechanical Emergency Door Release ............................. V-1
2. Ice Formation................................................. V-2
3. Troubleshooting by Guide ....................................... V-3
4. Information for the Customer Service ............................. V-4

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Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges
Technical Specifications ................................. A–1
1. Directives, Standards and Guidelines .............................A–2
2. Refrigerants ..................................................A–2
3. Mains Supply .................................................A–3
4. Water Supply .................................................A–3
5. Components..................................................A–4
Rotors ................................................ B–1
Items Supplied for Rotors .........................................B–1
HAEMAFlex 12 Rotor .............................................B–2
HAEMAFlex 16 Rotor .............................................B–3
Chemical Compatibility Chart ............................. C–1

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Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges
Table of Figures
Figure I–1: Centrifuge clearance, example with barcode scanner .......................................... I–2
Figure III–1: Overview centrifuge and rotor, e.g. HAEMAFlex 12 ...........................................III–2
Figure III–2: Align windshield knob and drive shaft .............................................................III–3
Figure III–3: Identify the circular marking, e.g. HAEMAFlex 8 and 12 ...................................III–3
Figure III–4: Rotor locking tool............................................................................................III–4
Figure III–5: Put the rotor locking tool. ................................................................................III–4
Figure III–6: Tighten the rotor to the drive shaft...................................................................III–5
Figure III–7: Lubricate trunnion pins, e.g. HAEMAFlex 12.....................................................III–5
Figure III–8: Install buckets into rotor, e.g. HAEMAFlex 12....................................................III–6
Figure III–9: Correctly assembled round bucket...................................................................III–6
Figure III–10: Correct Loading ............................................................................................III–7
Figure III–11: Incorrect Loading..........................................................................................III–7
Figure III–12: Insert windshield lid into holder ....................................................................III–8
Figure III–13: Select program...........................................................................................III–11
Figure III–14: Remove the rotor from the drive shaft..........................................................III–13
Figure IV–1: Cleaning rotor bolts and bucket bearing surfaces............................................ IV–4
Figure V–1: Marking on windshield lid .................................................................................V-1
Figure V–2: Position of the mechanical emergency door release...........................................V-2
Figure V–3: Example for an error message ..........................................................................V-3

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Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges
Table of Tables
Table Preface–1: Items Supplied ...........................................................................................vii
Table Preface–2: Signal Words and Colors..............................................................................ix
Table Preface–3: Symbols used on the centrifuge ................................................................. xiii
Table Preface–4: Symbols used in the manual ...................................................................... xiii
Table V–1: Error messages .................................................................................................V-3
Table A–1: Technical Data Cryofuge 16 Heavy Duty Water Cooled........................................A–1
Table A–2: Directives, Standards and Guidelines Cryofuge 16 Heavy Duty Water Cooled .......A–2
Table A–3: Refrigerants used in the Cryofuge 16 Heavy Duty Water Cooled..........................A–2
Table A–4: Mains Supply....................................................................................................A–3
Table A–5: Water Supply....................................................................................................A–3
Table A–6: Components.....................................................................................................A–4
Table B–1: Items supplied for rotors ...................................................................................B–1
Table B–2: HAEMAFlex 12 Rotor with Double Blood Bag Buckets.........................................B–2
Table B–3: HAEMAFlex 12 Rotor with Double Blood Bag Buckets.........................................B–3
Table C–1: Chemical Compatibility ...................................................................................C–11

Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges
Preface
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Preface
Before starting to use the centrifuge, read through this instruction manual carefully and follow the instructions.
Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the sellers
warranty.
Items Supplied
The centrifuges are supplied without a rotor. The items supplied with a rotor are listed in “Rotors” on page B–1.
Article No. Item Quantity
Centrifuge 1
75007677 Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled,
200, 208, 220, 230, 240 V ±10 %, 50 / 60 Hz, Single phase
50147062 Bottom Seal 1
50152646 Bottom Seal Connectors 2
50153114 Water Supply Kit 1
50152824 Cable Holder 1
50150042 Design Plugs 2
20510368 Screws 2
50153065 Cover Plate for Back Panel 1
50152178 Cover for Back Panel Connections 1
50153427 Cover Frame for Back Panel Connections 1
20310411 Plugs 7
20480317 Washers 7
20280119 Bubble Level 1
Instruction Manual 1
CD 1
Table Preface–1: Items Supplied
If any parts are missing, please contact the nearest Thermo Fisher Scientific representative.

Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges
Preface
viii
Intended Use for Cryofuge 16 Heavy Duty Water
Cooled
This centrifuge is intended for separation of blood into blood components using blood bags. The blood components are intended
to be used for human blood transfusion.
Clinical facilities and institutions are responsible for the process of human blood transfusion.
This intended use is limited to the associated rotors and Components. Based on the above this centrifuge is a medical device
per European Directive 93/42/EEC.
Intended User
The centrifuge must be operated by trained personal only.
Trained personnel can be a clinical laboratory technologist, a medical laboratory technician or personal with similar education.
Associated Rotors and Components
The Thermo Scientific™Cryofuge 16 Heavy Duty Water Cooled centrifuge can be operated with the Thermo Scientific™
HAEMAFlex™12 and HAEMAFlex 16 rotor. For more details on buckets and further Components refer to “Rotors” on page B–1
and “Components” on page A–4.

Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges
Preface
ix
Precautions
Signal Word and Color Degree of Hazard
WARNING Indicates a hazardous situation that, if not avoided, could result in death or serious injury.
CAUTION Indicates a hazardous situation that, if not avoided, could result in minor or moderate injury.
NOTICE Indicates information considered important, but not hazard-related.
Table Preface–2: Signal Words and Colors
WARNING
Observe the safety instructions. Not following these instructions can cause damage.
The centrifuge is to be used for its intended use only. Improper use can cause damages, contamination, and injuries with
fatal consequences.
The centrifuge must be operated by trained personnel only.
Thermo Fisher Scientific is not responsible for the process of human blood transfusion.
To ensure safe operation of this centrifuge regarding blood and blood components you have to follow the regulations in your
country.
CAUTION Due to air friction the temperature of rotor may raise significantly while the centrifuge is spinning. Refrigerated
units have limitations in cooling capabilities. Displayed and set temperature can deviate from sample temperature.
Sample temperature might exceed critical temperature of your application.
WARNING The magnets built into the rotors can have a negative effect on active implants, such as cardiac pacemakers.
The magnets are mounted on the bottom of the rotor.
Always keep a distance of 20 cm between the rotor and the active implant, as the product generates permanent
magnetic fields. The magnetic field strength at a distance of 20 cm is less than 0.1 mT, so there should be no
interference.
Set Up Conditions
WARNING
Plug the centrifuge only into sockets which have been properly grounded.
Turn off the centrifuge at the main switch. The mains plug must be freely accessible at all times.
Press the STOP key to shut down the centrifuge.
Pull out the power supply plug or disconnect the power supply in an emergency.
As safety zone maintain a clear radius of at least 30 cm around the centrifuge.
Do not place any dangerous substances within this safety zone.
Set up in a well-ventilated environment, on a horizontally levelled and rigid surface with adequate load-bearing capacity.
Use only the supplied water hoses and bottom seal connectors. Do not fixate the water hoses. Do not fixate the power
supply cable.
The safety zone can be violated and electrical hazard can occur if using other water hoses and bottom seal connectors
than the ones supplied.