Thermo Scientific Cryofuge 16 User manual

Thermo Scientific
Cryofuge 16
Heavy Duty Water Cooled Centrifuge
Instruction Manual
50152622-h • 03 / 2021
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WEEE Compliance
This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment
(WEEE) Directive 2012/19/EU. It is marked with the following symbol:
2

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Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges
Table of Contents
Preface .................................................vii
Items Supplied ...................................................vii
Intended Use for Cryofuge 16 Heavy Duty Water Cooled..................viii
Intended User....................................................................................................................... viii
Associated Rotors and Components ...................................................................................... viii
Precautions.......................................................ix
Set Up Conditions...................................................................................................................ix
Preparation .............................................................................................................................x
Hazardous Substances.............................................................................................................x
Operating...............................................................................................................................xi
Maintenance ..........................................................................................................................xi
Symbols used on the Centrifuge .....................................xii
Symbols used in the Manual ........................................xiii
Transport and Set Up .....................................I–1
1. Location ......................................................I–1
2. Transporting...................................................I–3
3. Storage.......................................................I–3
4. Shipping......................................................I–3
Thermo Scientific
Centri-Touch User Interface............................... II–1
Status .........................................................II–2
Run Parameters .................................................II–3
Control and Configuration .........................................II–3
Lighthouse Mode ................................................II–4
Operation .............................................III–1
1. Switch on the Centrifuge .......................................III–1
2. Open the Centrifuge Door .......................................III–1
3. Install a Rotor ................................................III–2
3. 1. Before Installation.................................................................................................III–2
3. 2. Rotor Installation ..................................................................................................III–2

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Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges
4. Maximum Loading.............................................III–8
Use of Tubes and Consumables...........................................................................................III–9
5. Close the Centrifuge Door.......................................III–9
6. Entering Parameters ...........................................III–9
6. 1. Acceleration / Deceleration Profiles .......................................................................III–9
6. 2. Preselect Speed / RCF-Value ................................................................................III–9
6. 3. Runtime Preselection..........................................................................................III–10
6. 4. Preselect Temperature........................................................................................III–10
6. 5. Pre-warm or Pre-cool the Centrifuge...................................................................III–10
7. Programs .................................................. III–11
8. Centrifugation .............................................. III–12
8. 1. Start ..................................................................................................................III–12
8. 2. Stop ..................................................................................................................III–12
9. Remove a Rotor ............................................. III–13
10. Switch off the Centrifuge .................................... III–13
Maintenance and Care ...................................IV–1
1. Cleaning Intervals ............................................ IV–1
2. Basics...................................................... IV–1
2. 1. Rotor and Components Inspection........................................................................ IV–2
3. Cleaning .................................................... IV–3
3. 1. Touchscreen ....................................................................................................... IV–4
3. 2. Filter Mat............................................................................................................ IV–5
4. Disinfection ................................................. IV–6
5. Decontamination ............................................. IV–6
6. Autoclaving ................................................. IV–7
7. Maintenance ................................................ IV–8
7. 1. Preventive Maintenance....................................................................................... IV–8
7. 2. Service ............................................................................................................... IV–8
8. Shipping and Disposal ........................................ IV–9
Troubleshooting .........................................V-1
1. Mechanical Emergency Door Release ............................. V-1
2. Ice Formation................................................. V-2
3. Troubleshooting by Guide ....................................... V-3
4. Information for the Customer Service ............................. V-4

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Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges
Technical Specifications ................................. A–1
1. Directives, Standards and Guidelines .............................A–2
2. Refrigerants ..................................................A–2
3. Mains Supply .................................................A–3
4. Water Supply .................................................A–3
5. Components..................................................A–4
Rotors ................................................ B–1
Items Supplied for Rotors .........................................B–1
HAEMAFlex 12 Rotor .............................................B–2
HAEMAFlex 16 Rotor .............................................B–3
Chemical Compatibility Chart ............................. C–1

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Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges
Table of Figures
Figure I–1: Centrifuge clearance, example with barcode scanner .......................................... I–2
Figure III–1: Overview centrifuge and rotor, e.g. HAEMAFlex 12 ...........................................III–2
Figure III–2: Align windshield knob and drive shaft .............................................................III–3
Figure III–3: Identify the circular marking, e.g. HAEMAFlex 8 and 12 ...................................III–3
Figure III–4: Rotor locking tool............................................................................................III–4
Figure III–5: Put the rotor locking tool. ................................................................................III–4
Figure III–6: Tighten the rotor to the drive shaft...................................................................III–5
Figure III–7: Lubricate trunnion pins, e.g. HAEMAFlex 12.....................................................III–5
Figure III–8: Install buckets into rotor, e.g. HAEMAFlex 12....................................................III–6
Figure III–9: Correctly assembled round bucket...................................................................III–6
Figure III–10: Correct Loading ............................................................................................III–7
Figure III–11: Incorrect Loading..........................................................................................III–7
Figure III–12: Insert windshield lid into holder ....................................................................III–8
Figure III–13: Select program...........................................................................................III–11
Figure III–14: Remove the rotor from the drive shaft..........................................................III–13
Figure IV–1: Cleaning rotor bolts and bucket bearing surfaces............................................ IV–4
Figure V–1: Marking on windshield lid .................................................................................V-1
Figure V–2: Position of the mechanical emergency door release...........................................V-2
Figure V–3: Example for an error message ..........................................................................V-3

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Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges
Table of Tables
Table Preface–1: Items Supplied ...........................................................................................vii
Table Preface–2: Signal Words and Colors..............................................................................ix
Table Preface–3: Symbols used on the centrifuge ................................................................. xiii
Table Preface–4: Symbols used in the manual ...................................................................... xiii
Table V–1: Error messages .................................................................................................V-3
Table A–1: Technical Data Cryofuge 16 Heavy Duty Water Cooled........................................A–1
Table A–2: Directives, Standards and Guidelines Cryofuge 16 Heavy Duty Water Cooled .......A–2
Table A–3: Refrigerants used in the Cryofuge 16 Heavy Duty Water Cooled..........................A–2
Table A–4: Mains Supply....................................................................................................A–3
Table A–5: Water Supply....................................................................................................A–3
Table A–6: Components.....................................................................................................A–4
Table B–1: Items supplied for rotors ...................................................................................B–1
Table B–2: HAEMAFlex 12 Rotor with Double Blood Bag Buckets.........................................B–2
Table B–3: HAEMAFlex 12 Rotor with Double Blood Bag Buckets.........................................B–3
Table C–1: Chemical Compatibility ...................................................................................C–11

Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges
Preface
vii
Preface
Before starting to use the centrifuge, read through this instruction manual carefully and follow the instructions.
Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the sellers
warranty.
Items Supplied
The centrifuges are supplied without a rotor. The items supplied with a rotor are listed in “Rotors” on page B–1.
Article No. Item Quantity
Centrifuge 1
75007677 Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled,
200, 208, 220, 230, 240 V ±10 %, 50 / 60 Hz, Single phase
50147062 Bottom Seal 1
50152646 Bottom Seal Connectors 2
50153114 Water Supply Kit 1
50152824 Cable Holder 1
50150042 Design Plugs 2
20510368 Screws 2
50153065 Cover Plate for Back Panel 1
50152178 Cover for Back Panel Connections 1
50153427 Cover Frame for Back Panel Connections 1
20310411 Plugs 7
20480317 Washers 7
20280119 Bubble Level 1
Instruction Manual 1
CD 1
Table Preface–1: Items Supplied
If any parts are missing, please contact the nearest Thermo Fisher Scientific representative.

Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges
Preface
viii
Intended Use for Cryofuge 16 Heavy Duty Water
Cooled
This centrifuge is intended for separation of blood into blood components using blood bags. The blood components are intended
to be used for human blood transfusion.
Clinical facilities and institutions are responsible for the process of human blood transfusion.
This intended use is limited to the associated rotors and Components. Based on the above this centrifuge is a medical device
per European Directive 93/42/EEC.
Intended User
The centrifuge must be operated by trained personal only.
Trained personnel can be a clinical laboratory technologist, a medical laboratory technician or personal with similar education.
Associated Rotors and Components
The Thermo Scientific™Cryofuge 16 Heavy Duty Water Cooled centrifuge can be operated with the Thermo Scientific™
HAEMAFlex™12 and HAEMAFlex 16 rotor. For more details on buckets and further Components refer to “Rotors” on page B–1
and “Components” on page A–4.

Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges
Preface
ix
Precautions
Signal Word and Color Degree of Hazard
WARNING Indicates a hazardous situation that, if not avoided, could result in death or serious injury.
CAUTION Indicates a hazardous situation that, if not avoided, could result in minor or moderate injury.
NOTICE Indicates information considered important, but not hazard-related.
Table Preface–2: Signal Words and Colors
WARNING
Observe the safety instructions. Not following these instructions can cause damage.
The centrifuge is to be used for its intended use only. Improper use can cause damages, contamination, and injuries with
fatal consequences.
The centrifuge must be operated by trained personnel only.
Thermo Fisher Scientific is not responsible for the process of human blood transfusion.
To ensure safe operation of this centrifuge regarding blood and blood components you have to follow the regulations in your
country.
CAUTION Due to air friction the temperature of rotor may raise significantly while the centrifuge is spinning. Refrigerated
units have limitations in cooling capabilities. Displayed and set temperature can deviate from sample temperature.
Sample temperature might exceed critical temperature of your application.
WARNING The magnets built into the rotors can have a negative effect on active implants, such as cardiac pacemakers.
The magnets are mounted on the bottom of the rotor.
Always keep a distance of 20 cm between the rotor and the active implant, as the product generates permanent
magnetic fields. The magnetic field strength at a distance of 20 cm is less than 0.1 mT, so there should be no
interference.
Set Up Conditions
WARNING
Plug the centrifuge only into sockets which have been properly grounded.
Turn off the centrifuge at the main switch. The mains plug must be freely accessible at all times.
Press the STOP key to shut down the centrifuge.
Pull out the power supply plug or disconnect the power supply in an emergency.
As safety zone maintain a clear radius of at least 30 cm around the centrifuge.
Do not place any dangerous substances within this safety zone.
Set up in a well-ventilated environment, on a horizontally levelled and rigid surface with adequate load-bearing capacity.
Use only the supplied water hoses and bottom seal connectors. Do not fixate the water hoses. Do not fixate the power
supply cable.
The safety zone can be violated and electrical hazard can occur if using other water hoses and bottom seal connectors
than the ones supplied.

Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges
Preface
x
Preparation
WARNING
It is the obligation of the operator to make sure, that the proper protective clothing is used. Mind the “Laboratory Biosafety
Manual” of the World Health Organization (WHO) and the regulations in your country.
Do not make any changes to the mechanical components of the centrifuge or rotor. Do not replace mechanical
components.
Do not touch the electronic components of the centrifuge. Do not make any changes to the electronic components.
Do not replace electronic components.
Use only a properly installed rotor. Follow the instructions in section “Install a Rotor” on page III–2.
Do not use rotors, buckets or Components which show any signs of removed protective coating, corrosion or cracks.
Contact customer service for further advice or inspections.
Use only with rotors which have been loaded properly.
Never overload the rotor.
Always balance the samples.
Use only rotors and Components for this centrifuge which have been approved by Thermo Fisher Scientific. Exceptions to
this rule are commercially available glass or plastic centrifuge labware, provided they have been designed to fit the rotor
or the adapter cavities and are approved for the speed or the RCF value of the rotor.
Make sure the rotor is locked properly into place before operating the centrifuge.
Implement measures which ensure that no one can approach the centrifuge for longer than absolutely necessary while it
is running.
Hazardous Substances
WARNING
Especially when working with corrosive samples (salt solutions, acids, bases), the Components and the centrifuge
chamber have to be cleaned thoroughly.
Do not centrifuge explosive or flammable materials or substances.
The centrifuge is neither inert nor protected against explosion. Never use the centrifuge in an explosion-prone
environment.
Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms without suitable safety precautions.
If centrifuging any hazardous materials mind the “Laboratory Biosafety Manual” of the World Health Organization (WHO) and
any local regulations. When centrifuging microbiological samples from the Risk Group II (according to the “Laboratory Biosafety
Manual” of the World Health Organization (WHO)), aerosol-tight biological seals have to be used. Look on the internet page of the
World Health Organization (www.who.int) for the “Laboratory Biosafety Manual”. For materials in a higher risk group, extra safety
measures have to be taken.
If toxins or pathogenic substances have contaminated the centrifuge or its parts, appropriate disinfection measures have
to be taken (“Disinfection” on page IV–6).
Extreme care should be taken with highly corrosive substances which can cause damage and impair the mechanical
stability of the rotor. These should only be centrifuged in fully sealed tubes.
If a hazardous situation occurs, turn off the power supply to the centrifuge and leave the area immediately.

Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges
Preface
xi
Operating
WARNING
Make sure that the power supply cable is set up correctly. Electrical Hazard can occur if the power supply cable is not
set up correctly.
Hang the power supply cable in loops on the cable holder at the backside of the centrifuge. The flag attached to the
power supply cable is marking the end of the necessary part of the power supply cable that needs to hang in loops on
the cable holder.
Never use the centrifuge if parts of its casing is damaged or missing.
Never start the centrifuge when the centrifuge door is open.
Do not move the centrifuge while it is running.
Do not lean on the centrifuge.
Do not place anything on top of the centrifuge during a run.
Never open the centrifuge door until the rotor has come to a complete stop and this has been confirmed on the
touchscreen.
The emergency door release may be used in emergencies only to recover the samples from the centrifuge, e.g. during
a power failure (“Mechanical Emergency Door Release” on page V-1).
Do not open the centrifuge, while it is running.
In any case of severe mechanical failure, such as rotor or bucket crash, the centrifuge is not aerosol-tight.
In case of rotor failure the centrifuge can be damaged. Leave the room. Inform customer service.
Maintenance
WARNING
The centrifuge housing is not to be opened by the operator.

Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges
Preface
xii
Symbols used on the Centrifuge
This symbol refers to general hazards. Observe the information contained in the instruction
manual to keep yourself and your environment safe.
CAUTION means that material damage could occur.
WARNING means that injuries or material damage or contamination could occur.
This symbol refers to biological hazards.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.
This symbol refers to hazards from sharp items.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.
This symbol refers to hazards from closing mechanical parts.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.
This symbol refers to information on hazards, described within the manual.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.
This symbol on rotors and Components refers to information on hazards, described within
the manual.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.
This symbol demands to disconnect mains before transporting or servicing the centrifuge.
This symbol shows the direction of rotation.
Wasser
water
eau
Eingang
input
entree
Ausgang
output
sortie
30V/1A max.
This symbol marks the inlet and outlet for the cooling liquid and
the connection to the floating contact (NO/NC).

Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges
Preface
xiii
Make sure that the power supply cable is set up correctly.
Electrical Hazard can occur if the power supply cable is not set up
correctly.
Hang the power supply cable in loops on the cable holder at the
backside of the centrifuge. The flag attached to the power supply
cable is marking the end of the necessary part of the power
supply cable that needs to hang in loops on the cable holder.
Table Preface–3: Symbols used on the centrifuge
Symbols used in the Manual
This symbol refers to general hazards. Observe the information contained in the instruction
manual to keep yourself and your environment safe.
CAUTION means that material damage could occur.
WARNING means that injuries or material damage or contamination could occur.
This symbol refers to biological hazards.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.
Table Preface–4: Symbols used in the manual

I–1
Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges
Transport and Set Up
THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS.
THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS.
Transport and Set Up
I
CAUTION Make sure that the centrifuge and the packaging is not damaged. Speak to the shipping
company and Thermo Fisher Scientific immediately if damage is found.
NOTICE Installation including relocation of the unit must be done by a Thermo Fisher Scientific
authorized service technician.
1. Location
WARNING Risk of impact.
The centrifuge can crush into objects and persons in a radius of 30 cm when spinning.
Keep a safety zone of 30 cm around the centrifuge for safe operation.
Make sure that no one is in the safety zone while the centrifuge spins.
WARNING Use only the supplied water hoses and bottom seal connectors. Do not fixate the water hoses.
Do not fixate the power supply cable.
The safety zone can be violated and electrical hazard can occur if using other water hoses and
bottom seal connectors than the ones supplied.
WARNING Make sure that the position of the opening in the floor and the centrifuge bottom for the water
and power supply installation are in an acceptable position to each other.
The safety zone can be violated and electrical hazard can occur if the position of the opening
in the floor and the centrifuge bottom for the water and power supply installation are not in an
acceptable position to each other. The installation requirements for the position of the openings
can be found within the architectural drawing (50152582). Contact a Thermo Fisher Scientific
authorized service technician if needed.
CAUTION UV rays decrease the stability of plastics.
Do not expose the centrifuge, rotors and plastic Components to direct sunlight.
Operate the centrifuge only inside a room.
The set-up location must fulfill these requirements:

I–2
Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges
Transport and Set Up
THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS.
THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS.
Keep a safety zone of minimum 30 cm (Figure I–1 on page I–2, green area) around the centrifuge.
Persons and hazardous substances must be kept out of this safety zone while centrifuging.
The supporting structure must:
»be stable and free of resonance,
»be applicable for horizontal setup of the centrifuge,
»hold the weight of the centrifuge.
Do not expose the centrifuge to heat and strong sunlight.
The set-up location must be well-ventilated at all times.
Figure I–1: Centrifuge clearance, example with barcode scanner

I–3
Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges
Transport and Set Up
THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS.
THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS.
2. Transporting
WARNING Never stand before a moving centrifuge to stop it.
If the centrifuge is moved on an uneven surface, it can get faster due to its own weight.
An impact of the centrifuge can lead to serious injuries.
CAUTION Always remove the rotor before you transport the centrifuge.
If you do not remove the rotor you can damage the centrifuge drive or drive shaft.
NOTICE Dispose the centrifuge packaging.
NOTICE Assign a shipping company for the transport.
Inform the customer service about the transport.
Impact can damage the centrifuge.
Send the centrifuge upright and if possible in packaging.
3. Storage
WARNING When you remove the centrifuge and Components from use, clean and if necessary disinfect
or decontaminate the full system. If you are not sure speak to the Thermo Fisher Scientific
customer service.
Before storing the centrifuge and the Components it must be clean and if necessary disinfected and decontaminated.
Centrifuge, rotors, buckets and Components have to be fully dry before storage.
Keep the centrifuge in a clean, dust-free location.
Keep the centrifuge on its rubber feet and not on its casters.
Do not store the centrifuge in direct sunlight.
4. Shipping
WARNING Before shipping the centrifuge and Components you must clean and if necessary disinfect
or decontaminate the full system. If you are not sure speak to the Thermo Fisher Scientific
customer service.
Before shipping the centrifuge:
The centrifuge must be clean and decontaminated.
You must confirm the decontamination with a decontamination certificate.

II–1
Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges
Thermo Scientific Centri-Touch User Interface
II
Thermo Scientific
Centri-Touch User Interface
➀Actual status information
➁Set values
➂Settings and navigation

II–2
Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges
Thermo ScientificCentri-Touch User Interface
Status
The upper part of the touchscreen displays the centrifuge status.
The remaining time is indicated while centrifugation is in progress. The progress bar shows the actual phase of the
centrifugation.
➀Status: In the Time mode, the remaining time for the centrifugation process is shown here. In the Hold
mode, the elapsed time is displayed.
➁Progress: The curve diagram is divided into the sections acceleration, centrifugation and deceleration.
➂Speed: The current speed of the rotor is shown here.
➃Temperature: The temperature in the centrifugation chamber is shown here.
Status that can be displayed
Ready Centrifugation can be started.
Door open Centrifuge door is open.
Door moving Door is automatically closing or opening.
Error An error has occurred.
Canceled Centrifugation has been stopped manually.
Complete Centrifugation has been successfully completed.
Pretempering
completed
Pretempering has been successfully completed.
No rotor No rotor is identified in the centrifuge.

II–3
Thermo Scientific Cryofuge 16 Heavy Duty Water Cooled Centrifuges
Thermo ScientificCentri-Touch User Interface
Run Parameters
The set points for centrifugation can be set in the parameterization window. Clicking one of these buttons will open a new window
in which you can input the appropriate set point value.
➀Acceleration: Select an acceleration profile (Level 1-10).
➁Time: Select the duration for centrifugation and the time mode.
➂Deceleration: Select the deceleration profile (Level 1-10).
➃Speed: Set the speed as rpm or RCF.
➄Temperature: Set the temperature (in °C) for the centrifugation chamber.
➅Program: Select a program for centrifugation. The previously selected program will always be displayed initially.
You can change the parameters once you have selected a program. Changing a parameter will cancel the
program.
➆Rotor: The rotor currently in use is displayed.
Control and Configuration
In this section you can start and halt centrifugation. You can also define settings, such as for the centrifugation programs. If you
are not sure of the use of a button you can use the tooltip mode, which provides information about all of the operator control
elements.
➀Start centrifugation: Centrifugation can be started using the current set values.
➁Open Door / Stop: When centrifugation starts, this button switches to Stop .
➂Configuration: The configuration menu is displayed when this button is clicked. This button is not active while
centrifugation is in progress.
➃Operator: In certain modes, touching the operator name opens a window for selecting other operators.
➄Tooltip Mode: This button is used to activate the tooltip mode. All functional elements are de-activated with
this mode. When an element is selected in the tooltip mode a field is displayed with information about
that particular element. If you press the tooltip button again, this mode is canceled.