Omega Bio-Tek Mag-Bind RXNPure Plus User manual

Mag-Bind® RXNPure Plus
M1386-01 50 mL
Manual Date: February 2023
Revision Number: v4.0

1
Introduction ....................................................................................2
Illustrated Protocol........................................................................3
Kit Contents/Preparations/Storage andStability................4
Mag-Bind® RXNPure Plus 96-well Plate Protocol................5
Mag-Bind® RXNPurePlus 384-wellPlateProtocol................8
Troubleshooting Guide.............................................................11
Manual Date: February 2023
Revision Number: v4.0
Mag-Bind® RXNPure Plus
Table of Contents

2
Introduction
Omega Bio-tek’s Mag-Bind® RXNPure Plus Kit allows rapid and reliable isolation of PCR
products with high recovery rates. The system combines Omega Bio-tek’s proprietary
chemistries with the reversible nucleic acid-binding properties of magnetic beads that
selectively binds PCR amplicons 100 bp and larger and eliminates excess nucleotides,
primers, and small, non-targeted amplication products, such as primer dimers. This kit
is designed for both manual and fully automated purication of PCR samples. Puried
PCR fragments can be used for microarrays, automated uorescent DNA sequencing,
restriction enzyme digestion, and other applications.
The Mag-Bind® RXNPure Plus magnetic particles technology provides a better solution
for nucleic acid purication compared to centrifugation and vacuum-based technologies.
The product can be easily scaled up while providing very user-friendly handling
procedures. If using Mag-Bind® RXNPure Plus for the rst time, please read this booklet
to become familiar with the procedures. PCR products are rst mixed with Mag-Bind®
RXNPure Plus. PCR products then selectively bind to the Mag-Bind® RXNPure Plus
particles. With two rapid wash steps, trace contaminants such as nucleotides, primers and
small, non-targeted amplication products are removed. Pure DNA is eluted in Elution
Buer or water. Puried DNA can be directly used in downstream applications without the
need for further purication.
Important: If automating this procedure on a liquid handler or a magnetic processor,
please contact your Omega Bio-tek representative for instrument-specic instructions.
New in this Edition:
February 2023
• M1386-00 (5 mL) and M1386-02 (500 mL) have been discontinued and are no longer
available to purchase.
March 2017
• General Revision

3
Illustrated Protocol
Measure the PCR Reaction
Add Mag-Bind®RXNPure Plus and Mix
Magnetize and Remove Supernatant
Wash Twice with 70% Ethanol
Dry
Elute DNA

4
Kit Contents
Storage and Stability
Mag-Bind® RXNPure Plus is guaranteed for at least 12 months from the date of purchase
when stored at 2-8°C.
Product Number M1386-01
Mag-Bind® RXNPure Plus 50 mL
User Manual P
Preparations
PCR Reaction Volume
96 well format
M1386-01
50 mL
10 μL 2,777 preps
25 μL 1,111 preps
50 μL 555 preps
100 μL 277 preps
PCR Reaction Volume
384 well format
M1386-01
50 mL
5 μL 5,555 preps
10 μL 2,777 preps
14 μL 1,984 preps

5
Mag-Bind® RXNPure Plus
96-well Plate Protocol
Important: If automating this procedure on a liquid handler or a magnetic processor,
please contact your Omega Bio-tek representative for instrument-specic instructions.
Materials and Equipment to be Supplied by User:
• Vortexer
• Magnetic Separation Device (Recommended Cat# AlpAqua A001322)
• Multichannel pipettor
• 96-well PCR plate containing PCR samples (up to 50 μL/well)
• 96-well microplate
• Multichannel Disposable Reservoirs
• Sealing lm
• 70% ethanol
• Elution Buer (Cat# PDR048 or 10 mM Tris, pH 8.5)
• Optional: Oven capable of 37°C
1. Read the manufacturer’s instruction manual for the magnetic separation device, if
provided.
2. Place the 96-well PCR plate containing PCR samples on the bench and measure the
volume of the PCR reaction. Determine the volume of Mag-Bind® RXNPure Plus that
will be added to the reaction. If the reaction volume will exceed 200 µL transfer to a
microtiter plate for processing.
Note: PCR reactions >20 µL will need to be transferred to a processing plate.
If processing in a PCR plate, a magnet separation device compatible with PCR plates
must be used (Recommend Cat# V&P Scientic VP 771H).
3. Shake or vortex the Mag-Bind® RXNPure Plus to resuspend any particles that may
have settled. Allow Mag-Bind® RXNPure Plus to come to room temperature before
use.

6
4. Add 1.8 volumes Mag-Bind® RXNPure Plus.
PCR Reaction Volume (μL) Mag-Bind®RXNPure Plus (μL)
10 18
25 45
50 90
100 180
5. Pipet up and down 5-10 times or vortex for 30 seconds.
6. Let sit for 5 minutes at room temperature.
7. Place the plate on a magnetic separation device to magnetize the Mag-Bind®
RXNPure Plus. Let sit at room temperature until the Mag-Bind® RXNPure Plus is
completely cleared from solution.
8. Aspirate and discard the cleared supernatant. Do not disturb the Mag-Bind® RXNPure
Plus. Leave the plate on the magnet.
9. Add 200 μL 70% ethanol.
10. Let sit at room temperature for 1 minute. It is not necessary to resuspend the Mag-
Bind® RXNPure Plus.
11. Aspirate and discard the cleared supernatant. Do not disturb the Mag-Bind® RXNPure
Plus.
12. Repeat Steps 9-11 for a second 70% ethanol wash step.
Mag-Bind® RXNPure Plus

7
13. Leave the plate on the magnetic separation device for 10-15 minutes to air dry the
Mag-Bind® RXNPure Plus. Remove any residual liquid with a pipettor.
Note: It is important to dry the Mag-Bind® RXNPure Plus before elution. Residual
ethanol may interfere with downstream applications.
Optional: Incubating the plate at 37°C can speed up the evaporation.
14. Remove the plate from magnetic separation device.
15. Add 30-40 µL Elution Buer (not provided).
16. Pipet up and down 20 times or vortex for 30 seconds.
17. Let sit for 2-3 minutes at room temperature.
18. Place the plate on a magnetic separation device to magnetize the Mag-Bind®
RXNPure Plus. Let sit at room temperature until the Mag-Bind® RXNPure Plus is
completely cleared from solution.
19. Transfer the cleared supernatant containing puried DNA to a new 96-well
microplate and seal with non-permeable sealing lm.
20. Store the plate at 2-8°C if storage is only for a few days. For long-term storage,
samples should be kept at -20°C.
Mag-Bind® RXNPure Plus

8
Mag-Bind® RXNPure Plus
384-well Plate Protocol
Important: If automating this procedure on a liquid handler or a magnetic processor,
please contact your Omega Bio-tek representative for instrument-specic instructions.
Materials and Equipment to be Supplied by User:
• Vortexer
• Magnetic separation device for 384-well PCR plates
• Multichannel pipettor
• 384-well PCR plate containing PCR samples (up to 100 μL/well)
• Skirted 384-well PCR plate
• 384-well microplate
• Multichannel Disposable Reservoirs
• Sealing lm
• 70% ethanol
• Elution Buer (Cat# PDR048 or 10 mM Tris, pH 8.5)
• Optional: Oven capable of 37°C
1. Read the manufacturer’s instruction manual for the magnetic separation device, if
provided.
2. Place the 384-well PCR plate on the bench and measure the volume of the PCR
reaction. Transfer the sample to a skirted 384-well PCR plate.
3. Shake or vortex the Mag-Bind® RXNPure Plus to resuspend any particles that may
have settled. Allow Mag-Bind® RXNPure Plus to come to room temperature before
use.
4. Add 1.8 volumes Mag-Bind® RXNPure Plus.
PCR Reaction Volume (μL) Mag-Bind®RXNPure Plus (μL)
5 9
10 18
14 25