BANDELIN Sonopuls HD 4050 User manual

Ultrasonic Homogeniser HD 4100
7
Ultrasonic Homogenisers
51376-012 en/2022-09
Operating Instructions
valid for:
HD 4050 Volume: 0.5 ml – 100 ml
HD 4100 Volume: 2 ml - 200 ml
HD 4200 Volume: 5 ml - 1000 ml
HD 4400 Volume: 100 ml - 3000 ml

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Copyright & limitation of liability
This document may not be reproduced, whether in full or in part, without the prior approval of BANDELIN electronic GmbH & Co. KG,
hereinafter "BANDELIN".
The German language original is the binding version of this document. Any differences in the translation are not binding and have no legal
effect. In case of any discrepancies between the translation and the original of this document, the original version will take precedence.
BANDELIN accepts no responsibility or liability for damage caused by improper handling or usage contrary to the intended purpose.
The documentation has been prepared with great care. Liability for indirect or direct damages arising because of incomplete or erroneous
information in this documentation, as well as in its delivery and usage, is excluded.
All illustrations are provided as examples and are not true to size. Decorative elements are not included in the scope of delivery.
Subject to technical alterations. Measurements are subject to manufacturing tolerances.
© 2022
1 2 GmbH & Co. KG, Heinrichstraße 3 – 4, Germany, 12207 Berlin,
Tel.: [phone] - 0, Fax: [phone], [email protected]

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General
The equipment, the accessories and the preparations are to be used in accordance with the user
instructions and/or the product information.
The instructions are part of the scope of delivery and are to be stored in the vicinity of the device
for later reference. This also applies if possession of the device is transferred elsewhere.
Before the device is put into operation, these instructions are to be read carefully and completely
in order for the user to become familiarised with all functions.
The warnings and safety precautions (chapter 1.5) are always to be followed during use.
The manufacturer will not assume any responsibility for the device's safety or functional ability
in the event of improper handling or usage contrary to the intended purpose. In the event
of unauthorised alterations/modifications, both the warranty claim and the CE conformity
will become void.
If service is required, please contact the authorised dealer or the manufacturer.
Symbols used:
Symbol Meaning Explanation
Danger Denotes information that, if not observed, could pose
a risk to life and limb, especially from electric shock.
Caution
Identifies information that must be observed and complied
with in order to prevent damage to the device or injury
to the user.
When device parts are labelled with this symbol,
reference must be made to the documentation.
Warning Warning of hot surface.
Important Identifies information that is important for execution.
Note Identifies explanatory information.
Wear ear protectors For health reasons, spending long periods of time in the vicinity
of the device without ear protectors is prohibited.
Instructions Identifies instructions that must be carried out
in the described sequence.

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Table of Contents
1 Product description .........................................................................................................6
1.1 Mode of operation ..........................................................................................................7
1.2 Intended purpose ...........................................................................................................7
1.3 CE conformity .................................................................................................................7
1.4 Technical data ................................................................................................................ 8
1.4.1 Ultrasound generator (GM) ............................................................................................8
1.4.2 Ultrasonic converter (UW) ..............................................................................................9
1.4.3 Other data .......................................................................................................................9
1.4.4 Electromagnetic compatibility (EMC) ...........................................................................10
1.4.5 Remote operation .........................................................................................................10
1.5 Warnings and safety instructions ..................................................................................12
2 Preparation ...................................................................................................................13
2.1 Scope of delivery ..........................................................................................................14
2.2 Setup / assembly ..........................................................................................................14
2.3 Putting into service .......................................................................................................15
3 Operation ......................................................................................................................15
3.1 Operating elements ......................................................................................................15
3.1.1 Switching the ultrasonic homogeniser on/off................................................................16
3.1.2 Explanation of the display fields ...................................................................................16
3.2 Setting the operating parameters .................................................................................16
3.2.1 Sonication time [Time set] ............................................................................................17
3.2.2 Pulsation [Pulsation] .....................................................................................................18
3.2.3 Temperature monitoring [Temperature] ........................................................................ 19
3.2.4 Miscellaneous functions and settings ...........................................................................20
3.3 Loading / saving sonication programs ..........................................................................21
3.4 Batch operation (batch processing) ..............................................................................22
3.5 Activating / deactivating the ultrasound ........................................................................23
4 Use ...............................................................................................................................24
4.1 Instructions for use .......................................................................................................24
4.2 General use ..................................................................................................................24
4.3 Selecting a suitable probe ............................................................................................26
4.3.1 Mounting and dismounting of the probes .....................................................................27

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4.3.1.1 Mounting and dismounting the titanium plates .............................................................28
4.3.1.2 Mounting and dismounting of the titanium probes ........................................................29
4.4 Dismounting and mounting the standard/booster horns ...............................................30
5 Cleaning and maintenance of the ultrasonic homogeniser ..........................................32
5.1 Cleaning and care ........................................................................................................32
5.2 Warehousing / storage .................................................................................................33
6 Maintenance and repair ................................................................................................34
6.1 Maintenance .................................................................................................................34
6.2 Functional checks .........................................................................................................34
6.2.1 Testing the ultrasonic converter (probe check) – Error 011 .......................................... 34
6.2.2 Conducting a frequency scan (scan frequency) – Error 002/011/012 .......................... 34
6.3 Troubleshooting ............................................................................................................35
6.4 Repairs and service ......................................................................................................37
6.4.1 Certificate of Decontamination ..................................................................................... 38
6.4.2 Software version display ..............................................................................................38
7 Accessories ..................................................................................................................39
7.1 Required accessories ...................................................................................................39
7.2 Optional accessories ....................................................................................................39
8 Consumable materials ..................................................................................................40
9 Taking the unit out of service ........................................................................................ 40
10 Keyword index – not applicable – .................................................................................40
Informative annexes
A Spanner for mounting/dismounting

11
12
10
78 9
13
4
6
4a
5
2
Example of use
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1 Product description
The SONOPULS ultrasonic homogeniser essentially consists of three device components:
the ultrasound generator, the ultrasonic converter, and the working tip (probe). The individual
components can be varied using a multitude of options and accessories. The type specification
and serial number are found on the type plate.
Product features:
• Ultrasound generator (1) in low-maintenance, robust plastic housing, with connections
for the ultrasonic converter and temperature sensor, and the recessed handle.
• Operating and display panel (2) with LCD display (3)
• Ultrasonic converter (4) with Start/Stop button (4a)
• Standard/booster horn (5), omitted for UW 50
• Probe (6)
• Mains switch (7)
• Connection (8) for ultrasonic converter – MINISNAP
• Connection for temperature sensor (9)
Rear side of the ultrasound generator:
• Cold device socket (10) with fuse holder
• Remote control connector (11)
• RS 232 interface (12)

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1.1 Mode of operation
The ultrasound generator transforms the mains energy input (mains frequency 50 or 60 Hz)
into high-frequency energy with a frequency of 20 kHz. The ultrasonic converter connected
to the ultrasound generator converts the high-frequency energy into ultrasound. The emitted
ultrasound has a high mechanical energy that is used for a wide range of applications.
Thus, mechanical deflections with the same frequency of 20 kHz are generated on the tip
of the probe and transferred into the sonication medium as ultrasonic waves with a high power
intensity. The amplitude is held constant via a signal feedback by the ultrasonic converter
(AMPLICHRON®circuitry), independently from the applied load, as long as the maximum
power allowed is not exceeded. Thanks to these measures, the reproducibility of the process
parameters is guaranteed and validation of the process is supported.
Standard/booster horns with probes are mounted on the ultrasonic converter. These work as
mechanical transformers and enable repeated mechanical augmentation of the ultrasound
amplitude at the tip.
1.2 Intended purpose
SONOPULS ultrasonic homogenisers generate high-performance ultrasound with high intensities
and ultrasound amplitudes that are transferred into liquid media using working tools called probes.
They are used in laboratories, clinics, and in industrial research, and they perform diverse tasks
during sample preparation in Quality Assurance, scientific experiments, analyses, and in pilot or
short-series manufacture.
Examples of use:
• Cell disruption for paternity tests
• Cell disruption (extraction of microorganisms, tissue cells)
• Homogenising of liquids
• Emulsifying hard-to-mix liquids
• Dispersion of agglomerates
• Acceleration of chemical reactions
• Degassing of liquids
• Sample preparation in environmental analytics (wastewater tests, soil samples)
• Sonochemistry
1.3 CE conformity
SONOPULS ultrasonic homogenisers satisfy the CE-marking criteria in the European Union:
- 2014 / 35 / EU - Low Voltage Directive
- 2014 / 30 / EU - EMC Directive
- 2011/ 65 / EU - RoHS Directive
A declaration of conformity can be requested from the manufacturer by providing the serial number.

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1.4 Technical data
SONOPULS ultrasonic homogenisers are interference-free and CE-marked.
Safety: EN 61010-1, EMC: EN 61326-1
1.4.1 Ultrasound generator (GM)
Mains supply: 230 V~ (± 10 %) 50/60 Hz or 115 V~ (± 10 %) 50/60 Hz
Protection class: I
Ultrasonic frequency: 20 kHz ± 500 Hz
Frequency control/1: automatic, resonance frequency search
Time setting range: 0:00:01 – 9:59:59 [h:mm:ss] or continuous operation
Ultrasound operating mode: pulsating or continuous
Pulsation time ON (tE): 0.2 - 600 s - (see chapter 3.2.2)
Pulsation time OFF (tA): 0.3 - 600 s - (see chapter 3.2.2)
Ultrasound control: amplitude or power - (see chapter 3.2.4)
Amplitude setting range/2: 10 to 100 % in 1 % steps
Amplitude/power display: default and progress bars
Energy display: kJ
Temperature sensor: optional (TM 50 or TM 100)
Temperature display: -10 to +120°C
Data memory locations: 9
Operating elements: foil button
Operating data display: LC graphic display, illuminated
Remote operation (turning on/off): button on the ultrasonic converter, potential-free contact,
foot switch TS 8 (optional)
Interface: RS 232
Degree of protection: IP 30 pursuant to DIN EN 60529
Generator:
Generator GM 4200 GM 4400
Power/3, maximum 50/100/200 W 200/400 W
Ultrasonic frequency 20 kHz
Power setting range 30 - 150 W 60- 300 W
Weight (approx.) 3.2 kg 3.6 kg
External dimensions (L × W × H) 335 × 150 × 230 mm
/1 Frequency control:
The ultrasound generator has an automatic search feature for resonance frequency and rate adaptation while in operation. Causes for
a change in the resonance frequency are, for example, a warming of the ultrasonic converter and the probes, a change in the acoustic
load due to changes in viscosity, and even the mounting of another probe. A frequency drift during operation is corrected by the automatic
frequency control. The optimum working frequency is sought using the resonance frequency search, e.g. after a change in probe.
/2 The minimum and maximum amplitude setting range depends on the respective probe type. The valid amplitude setting range is stored
in the program.
/3 Power:
In the case of amplitude control, the ultrasonic power that is needed for the desired probe amplitude is dependent on the viscosity of
the medium. In order to prevent damage to the ultrasound generator and converter, the power is limited to the maximum permitted peak
value. In the case of highly-viscous media, this can result in the desired amplitude not always being reached.
The power depends on the connected ultrasonic converter.

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1.4.2 Ultrasonic converter (UW)
UW 50 UW 100 UW 200 UW 400
Start/Stop key: 
Stable at continuous
operation: 
Frequency: 20 kHz 20 kHz 20 kHz 20 kHz
Weight: 0.6 kg 1.5 kg 1.6 kg 1.7 kg
Dimensions: Ø 50 × 190 mm Ø 70 × 170 mm Ø 70 × 170 mm Ø 90 × 180 mm
Degree of protection: IP 20 IP 20 IP 20 IP 20
1.4.3 Other data
Ambient conditions according to DIN EN 61 010-1
Overvoltage category: II
Degree of contamination: 2
Permissible relative humidity up to 31 °C: 80 %
Permissible relative humidity up to 40 °C: 50 %
Permissible ambient temperature: 5 to 40 °C
Altitude: up to 2000 m above sea level
No dewing.
Only for indoor operation.

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1.4.4 Electromagnetic compatibility (EMC)
The device has been checked for electromagnetic compatibility (EMC) in accordance with
DIN EN 61326-1 and meets the requirements for Class B devices in accordance with EN 55011.
Suitable for use in institutions and other areas that are directly connected to a public low-voltage
power supply, e.g., laboratory facilities.
1.4.5 Remote operation
Several options are available for remote control and remote operation. Depending
on requirements, the most convenient solution may be selected.
Operating
element Methods Functions Connection
1Key manual Ultrasonic operation ON/OFF Fixed on theultrasonic
converter
2Foot switch foot-operated Ultrasonic operation ON/OFF Remote control connector
3 External control signal • Ultrasonic operation ON/OFF
• Status confirmation
Remote control connector
4 External data protocol • Status inquiry
• Full device control
RS 232 interface
a) Remote control connector
Contact assignment and functional description of the remote control connector
Diagram Contact Assignment Signal Function
1
2
3
45
1 Input 0V L
5V H
L Ultrasound operation ON
H Ultrasound operation
OFF
2 Output 0V L
5V H
L Ultrasound operation OFF
H Ultrasound operation ON
3 Earth
4 Output like contact 2
5 n.a.
Notes:
• The connection of power or voltage sources of any type is not permitted.
• The source resistance for the outputs is of 3.3 kΩ. The input resistance for subsequent signal
inputs, e.g. optocouplers, must be greater than or equal to 47 kΩ.
• The function of the signal input (contact 1) depends on the operating mode that is set for
the pulsation (see chapter 3.2.2). With the operating mode "by hand key", control is performed
statically, i.e. the ultrasonic operation is always activated under L status, and deactivated under
H status.
With all other operating modes, the input works as an edge-controlled changeover switch.
The input resistance totals approx. 50 kΩ.
• When connecting a changeover contact (toggle function) to connector 1, the make time
must be > 100 ms.

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b) RS 232 interface
The RS 232 interface connection is located on the rear side of the ultrasound generator.
A standard serial conductor (RS 232) can be used for connection and communication
purposes. Implementation of the technical programs required for communication is
the responsibility of the operator and is not supported by the manufacturer. The manufacturer
only guarantees the proper functioning of the interface.
The instruction set for the RS-232 interface will be made available by the manufacturer free
of charge, upon request.
Note:
Proper operation will depend on the length of the connection cable. If an extension is used,
a length of 5 m may not be exceeded.
Caution!
The inputs and outputs of the remote control connector and RS-232 interface may only
be connected with devices of protection class I that are connected to the same mains
supply circuit.

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1.5 Warnings and safety instructions
General
• Keep the device and accessories out of the reach of children and also of persons
who have not been instructed in their operation by reference to these instructions.
• The use of the device or parts thereof on humans or animals is not authorised.
• Keep the ultrasound generator and operating elements clean and dry.
• Do not expose the device to corrosive materials.
• It is imperative that you observe the hygiene instructions when working with the device.
• The connection of any type of power or voltage sources to the signal inputs or outputs
is forbidden.
• Signal wires for foot switches, temperature sensors, etc., may not exceed a maximum
length of 3 m.
• All plug connections (such as for ultrasonic converters, foot switches) may only be plugged
or unplugged while the device is turned offor in idle mode (pause mode).
• The ultrasound generator and ultrasonic converter must be transported separately.
• Ultrasonic homogenisers adhere to prescribed EMC limit values, so that it can be assumed that
the electromagnetic radiation emanating from the devices is harmless to humans. A binding
statement for wearers of implants can only be made at the place of work together with
the implant manufacturer. In case of doubt, information regarding the allowable
electromagnetic exposure level should be obtained from the implant manufacturer.
Operation
• Observe environmental and set-up conditions, see chapter 1.4.
• Determine the mains voltage before connecting the ultrasound generator.
• Only connect the ultrasound generator to an earthed socket.
Fuse protection 16 A (main fuse).
• The ultrasonic converter may only be firmly mounted onto the black housing using a suitable
support.
• Before each start-up, check that the probe is firmly positioned on the standard or booster horn;
if necessary, tighten the probe (see chapter 4.3.1).
• Do not touch any oscillating parts (e.g. standard or booster horns, micro tips or tapered tips,
titanium plates, ultrasonic converter) during operation! Damage to health is possible.
• Do not touch the sonication vessels with the oscillating probe - probes and vessels could
be damaged.
• The sonication of liquids generates noise. Suitable accessories such as a sound proof box,
significantly reduce this noise. If operating without a sound proof box, wear hearing protection.
• Warning, risk of splashing!
This is especially the case with small sample quantities and when immersing oscillating probes.
• Do not use combustible solvents in open reaction vessels since the operating safety of the
ultrasonic homogeniser could be compromised. Safe extraction of combustible vapours must
be guaranteed. When using a sound proof box, the vapours cannot escape.
• Before any mounting or dismounting (chapters 4.3 and 4.4) of probes or standard booster horns
as well as accessories, turn offthe device and disconnect the ultrasonic converter from the
ultrasound generator.
• Only use the prescribed tool for mounting and dismounting (see appendix A).
• Do not use any bent probes (unstable operation, loss of power).

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• Liquids must not penetrate the inside of the ultrasonic converter or ultrasound generator.
• Never twist the ultrasonic converter's housing toward the aluminium cylinder
(ultrasonic oscillating system). The ultrasonic oscillating system and its electrical
connections would be damaged as a result.
• Do not operate the device without supervision.
• The ultrasonic homogeniser is only intended for use by qualified personnel.
• In isolated cases, unfavourable operating conditions may result in EMC disruptions
that may affect devices that are in the direct vicinity.
• During operation, portable or mobile HF communication systems in the vicinity
of the ultrasonic homogeniser should be turned off- operation may be disrupted.
Damage
• If damage to the ultrasonic homogeniser is detected, do not connect the ultrasonic
homogeniser to the mains.
• In the event of defects, disconnect the mains plug immediately.
• Repairs must only be conducted by authorised skilled personnel or by the manufacturer.
• Defective parts must only be replaced with original parts or parts of the same quality!
2 Preparation
Carefully unpack the ultrasound generator, ultrasonic converter and accessories, and inspect
them for completeness or possible transportation damage. If damage or defects are ascertained,
notify this to the forwarding agent and the supplier immediately and in writing.
Before start-up, the ultrasonic homogeniser should be allowed to stand for 2 hours at its operating
location so that it can adjust to the ambient conditions.

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2.1 Scope of delivery
The scope of delivery depends on the order size. In general, it comprises the following order parts:
1 Ultrasound generator (GM) ...
1 Mains cable, 2 m
1 Ultrasonic converter UW ...
1 Standard/booster horn ...
1 Probe
1 Tool set
1 Operating instructions
Additional accessories in accordance with order.
2.2 Setup / assembly
Place the ultrasound generator atop a firm, level and dry surface. In doing so,
• do not group it or stack it over other electrical or electronic devices,
• do not cover the ventilation holes on the ultrasonic converter's housing,
• guard against moisture and wetness - risk of electric shock.
The ultrasound generator must be positioned in such a way that disconnection of the power
supply is easy.
On delivery, the standard/booster horn is, if necessary, already firmly screwed
to the corresponding ultrasonic converter. Other standard/booster horns can
be mounted, see the notes in chapter 4.4.
Screw the delivered probe to the standard/booster horn, see chapter 4.3.1.
Position the ultrasonic converter safely and correctly in a suitable holder.
For this purpose, firmly clamp the ultrasonic converter only onto the black housing.
Position the ultrasonic converter and holder in a sound proof box.
The ultrasonic converter is connected to the ultrasound generator through a push-pull circular
connector with lock (MINISNAP). To connect, hold the mains connector from the handle and
position it in such a way that the arrow points toward the left. The mains connector can only
be plugged in when in this position. Plug the mains connector into the socket up to the limit,
and let it snap into place. In order to unplug the connection, hold the mains connector only
from the handle, pull it back and remove the connector. If necessary, see chapter1.
Verify that the mains switch is in the "0" position.
Before the mains connection, identify the mains voltage at hand and compare it with
the voltage specified on the type plate on the generator bottom. Connect to a grounded
socket only if type is suitable.

Solid green: Ultrasound emission
Blinking green: Remote operation
through the RS 232
interface
or
Solid red: Error messages
Blinking red: Warning messages
1 LCD Display Display of the operating parameters and status information
2 "START/STOP" key Starting or stopping the ultrasound emission, ending functions,
exiting the menu
3 "SET" key Call-up of the menu. Navigation within the menu
4 "Arrow" keys Setting the menu or operating parameters
5 Control LED
1
5
3
4
2
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2.3 Putting into service
Check the firm positioning of the probe and, if needed, clean thoroughly.
Check the positioning of the ultrasonic converter in the holder.
Connect the ultrasound generator to the mains (earthed socket) and switch on.
Set the probe type (see chapter 3.1.1).
Conduct a function test in accordance with chapter 6.2.1, if required
(e.g. after replacing the ultrasonic converter).
Connect the temperature sensor if necessary.
3 Operation
3.1 Operating elements
Operation is conducted from the operating and display panel on the front side
of the ultrasound generator:

UW 50 →HD 4050
UW 100 →HD 4100
UW 200 →HD 4200
UW 400 →HD 4400
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3.1.1 Switching the ultrasonic homogeniser on/off
The ultrasonic homogeniser is turned on with the mains switch (front side, bottom). After turning
on, the LCD display must light up. Initialisation occurs automatically. The ultrasonic homogeniser
will display the manufacturer's information, type designation, and the last-connected probe.
The type designation depends on the ultrasonic converter that is connected
BANDELIN electronic
HD 4050
Converter: UW 50
Probe: TS 106
o.k. −►[start]
If the probe type displayed does not conform with the probe that is mounted, the correct probe type
will have to be set using the arrow keys before the next steps are performed. By pressing
the "START/STOP" button, the menu switches into stand-by mode (pause mode) and the ultrasonic
homogeniser is ready for operation. The parameters used during the last operation will be set again
and displayed. If the ultrasonic converter was exchanged in the meantime, the earlier settings will be
replaced by standard values and must be re-edited. The temperature display is only visible when
a temperature sensor is connected.
Prog 3* Ampl 10%
Time set 00:01:00
elapsed 00:00:00
Pulsation off
Energy 0.000 kJ
The mains switch can also be used for powering off.
The powering-offprocess may take a few seconds while the current data is saved internally.
The monitor is turned offduring this process. When powered-on again, the data becomes
available once again.
3.1.2 Explanation of the display fields
Prog 3* Ampl 10%
Time set 00:01:00
00:00:04
Pulsation off
Energy 0.000 kJ
Default value
Amplitude (Am) in %
or power (Pw) in W
Default value
Sonication time
Hours:Minutes:Seconds
(Display omitted during continuous operation)
Energy applied
to the medium
Default value
Pulsation time OFF
(specification omitted with continuous
operation)
Time elapsed
Hours:Minutes:Seconds
Program No.
With modified programs, a "*"
appears behind the No.
3.2 Setting the operating parameters
With the exception of the amplitude and power, the operating parameters can only be set while
in stand-by mode (pause mode). In order to set or edit the parameters, the desired field can be
selected by pressing the "SET" key. The respective function can then be selected using the arrow
keys . If the parameter has number values that can be edited, the editing mode is then
activated with the arrow key . In the marked area (inverse view) of the parameter, the desired
value can then be set with the arrow keys . It is possible to move between individual setting
fields within the parameter using the arrow key . Editing mode is cancelled with the "SET"
or "START" keys.

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The following operating parameters can be set:
• Default values for the relative amplitude or power ([Ampl [%] or Power [W])
• Default values for the sonication time (Time set [hh:mm:ss])
• Default values for the pulsation of the ultrasound (turn-on and turn-offtime) (Pulsation [s])
• Limit value for the temperature monitoring [°C]
The operating parameters "Energy" and "Elapsed sonication time" cannot be edited.
3.2.1 Sonication time [Time set]
A value of 9 hours, 59 minutes and 59 seconds (9:59:59) is the maximum sonication time
that can be set. If the default value is exceeded during ultrasonic operation, the sonication
will stop. The parameter "non-stop" is equivalent to unlimited continuous operation until
ultrasonic operation is ended by pressing the "START/STOP" key.
a) Setting continuous operation [non-stop]
SET
Prog 1 Ampl 10%
Time set 00:01:00
elapsed 00:00:00
Pulse 4.0s 4.0s
Stop at 0°C ---°C
Energy 0.000 kJ
Time set 00:01:00
Prog 1 Ampl 10%
Time set 00:01:00
elapsed 00:00:00
Pulse 4.0s 4.0s
Stop at 0°C ---°C
Energy 0.000 kJ
Time set nonstop
b) Setting the sonication time
If a value of 59 minutes or seconds is exceeded when setting the time, or if a shortfall occurs when
setting back the time, the values for the set hours or minutes will change accordingly. Example:
SET
Prog 1 Ampl 10%
Time set 00:01:00
elapsed 00:00:00
Pulse 4.0s 4.0s
Stop at 0°C ---°C
Energy 0.000 kJ
Time set 00:01:00
Prog 1 Ampl 10%
Time set 00:01:00
elapsed 00:00:00
Pulse 4.0s 4.0s
Stop at 0°C ---°C
Energy 0.000 kJ
00:01
Prog 1* Ampl 10%
Time set 00:01:00
elapsed 00:00:00
Pulse 4.0s 4.0s
Stop at 0°C ---°C
Energy 0.000 kJ
00:02
Prog 1* Ampl 10%
Time set 00:01:00
elapsed 00:00:00
Pulse 4.0s 4.0s
Stop at 0°C ---°C
Energy 0.000 kJ
00:02:00
Prog 1* Ampl 10%
Time set 00:01:00
elapsed 00:00:00
Pulse 4.0s 4.0s
Stop at 0°C ---°C
Energy 0.000 kJ
00:02:15
or
START
STOP back to stand-by mode
SET
proceed to next set value or operating parameter

Prog 1* Ampl 10%
Time set 00:02:15
elapsed 00:00:00
Pulse 4.0s 4.0s
Stop at 0°C ---°C
Energy 0.000 kJ
4
Prog 1* Ampl 10%
Time set 00:02:15
elapsed 00:00:00
Pulse 5.1s 4.0s
Stop at 0°C ---°C
Energy 0.000 kJ
4
Prog 1* Ampl 10%
Time set 00:02:15
elapsed 00:00:00
Pulse 5.1s 4.0s
Stop at 0°C ---°C
Energy 0.000 kJ
4
Prog 1* Ampl 10%
Time set 00:02:15
elapsed 00:00:00
Pulsation off
Stop at 0°C ---°C
Energy 0.000 kJ
Pulsation 4.0s 4.0s
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1electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • [email protected]
3.2.2 Pulsation [Pulsation]/4
In addition to setting the pulse turn-on time tEand the pulse turn-offtime tA, two additional
operating modes can also be set:
offno pulsation or continuous sound
by hand key manual pulsation can be performed with the button on the ultrasonic converter.
Ultrasound operation is active as long as the button on the ultrasonic converter
is pressed.
a) Setting the operating modes
SET
Prog 1* Ampl 10%
Time set 00:02:15
elapsed 00:00:00
Pulsation off
Stop at 0°C ---°C
Energy 0.000 kJ
Pulsation off
Prog 1* Ampl 10%
Time set 00:02:15
elapsed 00:00:00
Pulsation off
Stop at 0°C ---°C
Energy 0.000 kJ
Pulsation by handkey
Prog 1* Ampl 10%
Time set 00:02:15
elapsed 00:00:00
Pulsation off
Stop at 0°C ---°C
Energy 0.000 kJ
Pulsation 4.0s 4.0s
b) Setting the time intervals
The maximum setting value is 600.0 s. The minimum setting value will depend on the type
of device and ranges between 0.1 und 0.3 s.
Turn-on time tE
Turn-offtime tA
START
STOP back to stand-by mode
SET
proceed to next operating parameter
/4 The pulse period duration is the sum of the turn-on and turn-offtime t = tE+ tA

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1electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • [email protected]
3.2.3 Temperature monitoring [Temperature]
The temperature in the sonicating medium can be monitored using the temperature sensor.
The following functions can be set:
offno monitoring
stop when reaching or exceeding the set temperature limit value, the ultrasound operation
will be turned off. If the temperature falls below the limit value once again, the ultrasound
operation will not automatically continue.
alarm when reaching or exceeding the set temperature limit value, an acoustic and visual
warning signal (red blinking LED) will be triggered. The ultrasound operation will not be
turned off.
a) Setting the type of monitoring
SET
Prog 3* Ampl 10%
Time set 00:00:15
elapsed 00:00:00
Pulsation off
Temperature ---°C
Energy 0.000 kJ
Temperature ---°C
Prog 3* Ampl 10%
Time set 00:00:15
elapsed 00:00:00
Pulsation off
Alarm at 0°C ---°C
Energy 0.000 kJ
Alarm at 0°C ---°C
Prog 3* Ampl 10%
Time set 00:00:15
elapsed 00:00:00
Pulsation off
Stop at 0°C ---°C
Energy 0.000 kJ
Stop at 0°C ---°C
Example:
SET
Prog 3* Ampl 10%
Time set 00:00:15
elapsed 00:00:00
Pulsation off
Temperature ---°C
Energy 0.000 kJ
Temperature ---°C
Prog 3* Ampl 10%
Time set 00:00:15
elapsed 00:00:00
Pulsation off
Alarm at 0°C ---°C
Energy 0.000 kJ
Alarm at 0°C ---°C
Prog 3* Ampl 10%
Time set 00:00:15
elapsed 00:00:00
Pulsation off
Alarm at 0°C ---°C
Energy 0.000 kJ
Alarm at 60°C ---°C
Prog 3* Ampl 10%
Time set 00:00:15
elapsed 00:00:00
Pulsation off
Alarm at 0°C ---°C
Energy 0.000 kJ
Stop at 60°C ---°C
Prog 3* Ampl 10%
Time set 00:00:15
elapsed 00:00:00
Pulsation off
Stop at 0°C ---°C
Energy 0.000 kJ
0°C
Prog 3* Ampl 10%
Time set 00:00:15
elapsed 00:00:00
Pulsation off
Stop at 0°C ---°C
Energy 0.000 kJ
60°C
The maximum setting value is 120 °C, the minimum is -10 °C.
As shown in the example, the values for the Alarm and Stop functions are carried over when
defining a limit temperature. An alarm is triggered simultaneously in Stop mode.
Temperature measured at
temperature sensor
no sensor: ---°C
Prog 1* Ampl 20%
Time set 00:01:00
00:00:09
Pulsation 4.0s 4.0s
Temperature 31°C
Energy 0.000 kJ
SET
or
START
STOP back to stand-by mode

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1electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • [email protected]
3.2.4 Miscellaneous functions and settings
Additional functions are available within a second menu level. These can be accessed by
simultaneously pressing the "SET" key and arrow key . Switching between these functions is
accomplished with the "SET" key in the manner already described. The menu level can only be
exited by pressing the "START/STOP" key. In doing so, settings and function statuses are carried
over and executed.
probe check off
scan frequency off
contrast 22
operating mode Ampl.
energy display
batch mode off
03.01 - 2016-08-085
off Check the ultrasonic converter
Search resonance frequency
Set monitor contrast
Set type of ultrasound control
Check the ultrasonic converter [probe check]
This function is used for quick verification of whether the ultrasonic converter is recognised and
can be correctly activated by the ultrasound generator. After a change in probe or in operating
conditions it is possible, for example, that the ultrasound generator cannot synchronise with
the ultrasonic converter and issues an error message, e.g. Error 011. The frequency is reset
to the base value and the function is restored if no device fault or other cause is present.
The default value is "off". In order to activate the function, set the parameter to "start" using
the arrow keys , and then press the "START/STOP" key. See chapter 6.2.1 for
additional information.
Search for resonance frequency [scan frequency]
This function is an expansion of the above-mentioned "probe check" function. In addition,
a frequency scan is conducted in order to determine the correct ultrasonic frequency for
the ultrasonic converter. Depending upon the probe used and the operating conditions,
the frequency can fluctuate significantly at times. When switched off, the ultrasound generator
saves the current frequency value and uses it as the start value when switched on again.
If there are significant deviations in the current frequency, this can lead to malfunctions.
Note:
After a successful scan while in continuous operation (pulsation = off, time > 30 s), start with
an amplitude setting of 50 % and check the operation. Next, set the desired default value.
Setting the monitor contrast [contrast]
With this function, the monitor contrast can be adapted to the local light conditions. The contrast
values range between 1 and 68, and can be set with the arrow keys . Smaller values result
in a darker image and larger values result in a lighter image. The default value is 22.
probe check off
scan frequency off
contrast 22
operating mode Ampl.
energy display
batch mode off
03.01 - 2016-08-085
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