BANDELIN Sonopuls HD 4050 User manual

Ultrasonic Homogeniser HD 4100
7
Ultrasonic Homogenisers
51376-012 en/2022-09
Operating Instructions
valid for:
HD 4050 Volume: 0.5 ml – 100 ml
HD 4100 Volume: 2 ml - 200 ml
HD 4200 Volume: 5 ml - 1000 ml
HD 4400 Volume: 100 ml - 3000 ml

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1electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • [email protected]
Copyright & limitation of liability
This document may not be reproduced, whether in full or in part, without the prior approval of BANDELIN electronic GmbH & Co. KG,
hereinafter "BANDELIN".
The German language original is the binding version of this document. Any differences in the translation are not binding and have no legal
effect. In case of any discrepancies between the translation and the original of this document, the original version will take precedence.
BANDELIN accepts no responsibility or liability for damage caused by improper handling or usage contrary to the intended purpose.
The documentation has been prepared with great care. Liability for indirect or direct damages arising because of incomplete or erroneous
information in this documentation, as well as in its delivery and usage, is excluded.
All illustrations are provided as examples and are not true to size. Decorative elements are not included in the scope of delivery.
Subject to technical alterations. Measurements are subject to manufacturing tolerances.
© 2022
1 2 GmbH & Co. KG, Heinrichstraße 3 – 4, Germany, 12207 Berlin,
Tel.: [phone] - 0, Fax: [phone], [email protected]

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General
The equipment, the accessories and the preparations are to be used in accordance with the user
instructions and/or the product information.
The instructions are part of the scope of delivery and are to be stored in the vicinity of the device
for later reference. This also applies if possession of the device is transferred elsewhere.
Before the device is put into operation, these instructions are to be read carefully and completely
in order for the user to become familiarised with all functions.
The warnings and safety precautions (chapter 1.5) are always to be followed during use.
The manufacturer will not assume any responsibility for the device's safety or functional ability
in the event of improper handling or usage contrary to the intended purpose. In the event
of unauthorised alterations/modifications, both the warranty claim and the CE conformity
will become void.
If service is required, please contact the authorised dealer or the manufacturer.
Symbols used:
Symbol Meaning Explanation
Danger Denotes information that, if not observed, could pose
a risk to life and limb, especially from electric shock.
Caution
Identifies information that must be observed and complied
with in order to prevent damage to the device or injury
to the user.
When device parts are labelled with this symbol,
reference must be made to the documentation.
Warning Warning of hot surface.
Important Identifies information that is important for execution.
Note Identifies explanatory information.
Wear ear protectors For health reasons, spending long periods of time in the vicinity
of the device without ear protectors is prohibited.
Instructions Identifies instructions that must be carried out
in the described sequence.

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Table of Contents
1 Product description .........................................................................................................6
1.1 Mode of operation ..........................................................................................................7
1.2 Intended purpose ...........................................................................................................7
1.3 CE conformity .................................................................................................................7
1.4 Technical data ................................................................................................................ 8
1.4.1 Ultrasound generator (GM) ............................................................................................8
1.4.2 Ultrasonic converter (UW) ..............................................................................................9
1.4.3 Other data .......................................................................................................................9
1.4.4 Electromagnetic compatibility (EMC) ...........................................................................10
1.4.5 Remote operation .........................................................................................................10
1.5 Warnings and safety instructions ..................................................................................12
2 Preparation ...................................................................................................................13
2.1 Scope of delivery ..........................................................................................................14
2.2 Setup / assembly ..........................................................................................................14
2.3 Putting into service .......................................................................................................15
3 Operation ......................................................................................................................15
3.1 Operating elements ......................................................................................................15
3.1.1 Switching the ultrasonic homogeniser on/off................................................................16
3.1.2 Explanation of the display fields ...................................................................................16
3.2 Setting the operating parameters .................................................................................16
3.2.1 Sonication time [Time set] ............................................................................................17
3.2.2 Pulsation [Pulsation] .....................................................................................................18
3.2.3 Temperature monitoring [Temperature] ........................................................................ 19
3.2.4 Miscellaneous functions and settings ...........................................................................20
3.3 Loading / saving sonication programs ..........................................................................21
3.4 Batch operation (batch processing) ..............................................................................22
3.5 Activating / deactivating the ultrasound ........................................................................23
4 Use ...............................................................................................................................24
4.1 Instructions for use .......................................................................................................24
4.2 General use ..................................................................................................................24
4.3 Selecting a suitable probe ............................................................................................26
4.3.1 Mounting and dismounting of the probes .....................................................................27

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4.3.1.1 Mounting and dismounting the titanium plates .............................................................28
4.3.1.2 Mounting and dismounting of the titanium probes ........................................................29
4.4 Dismounting and mounting the standard/booster horns ...............................................30
5 Cleaning and maintenance of the ultrasonic homogeniser ..........................................32
5.1 Cleaning and care ........................................................................................................32
5.2 Warehousing / storage .................................................................................................33
6 Maintenance and repair ................................................................................................34
6.1 Maintenance .................................................................................................................34
6.2 Functional checks .........................................................................................................34
6.2.1 Testing the ultrasonic converter (probe check) – Error 011 .......................................... 34
6.2.2 Conducting a frequency scan (scan frequency) – Error 002/011/012 .......................... 34
6.3 Troubleshooting ............................................................................................................35
6.4 Repairs and service ......................................................................................................37
6.4.1 Certificate of Decontamination ..................................................................................... 38
6.4.2 Software version display ..............................................................................................38
7 Accessories ..................................................................................................................39
7.1 Required accessories ...................................................................................................39
7.2 Optional accessories ....................................................................................................39
8 Consumable materials ..................................................................................................40
9 Taking the unit out of service ........................................................................................ 40
10 Keyword index – not applicable – .................................................................................40
Informative annexes
A Spanner for mounting/dismounting

11
12
10
78 9
13
4
6
4a
5
2
Example of use
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1electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • [email protected]
1 Product description
The SONOPULS ultrasonic homogeniser essentially consists of three device components:
the ultrasound generator, the ultrasonic converter, and the working tip (probe). The individual
components can be varied using a multitude of options and accessories. The type specification
and serial number are found on the type plate.
Product features:
• Ultrasound generator (1) in low-maintenance, robust plastic housing, with connections
for the ultrasonic converter and temperature sensor, and the recessed handle.
• Operating and display panel (2) with LCD display (3)
• Ultrasonic converter (4) with Start/Stop button (4a)
• Standard/booster horn (5), omitted for UW 50
• Probe (6)
• Mains switch (7)
• Connection (8) for ultrasonic converter – MINISNAP
• Connection for temperature sensor (9)
Rear side of the ultrasound generator:
• Cold device socket (10) with fuse holder
• Remote control connector (11)
• RS 232 interface (12)

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1.1 Mode of operation
The ultrasound generator transforms the mains energy input (mains frequency 50 or 60 Hz)
into high-frequency energy with a frequency of 20 kHz. The ultrasonic converter connected
to the ultrasound generator converts the high-frequency energy into ultrasound. The emitted
ultrasound has a high mechanical energy that is used for a wide range of applications.
Thus, mechanical deflections with the same frequency of 20 kHz are generated on the tip
of the probe and transferred into the sonication medium as ultrasonic waves with a high power
intensity. The amplitude is held constant via a signal feedback by the ultrasonic converter
(AMPLICHRON®circuitry), independently from the applied load, as long as the maximum
power allowed is not exceeded. Thanks to these measures, the reproducibility of the process
parameters is guaranteed and validation of the process is supported.
Standard/booster horns with probes are mounted on the ultrasonic converter. These work as
mechanical transformers and enable repeated mechanical augmentation of the ultrasound
amplitude at the tip.
1.2 Intended purpose
SONOPULS ultrasonic homogenisers generate high-performance ultrasound with high intensities
and ultrasound amplitudes that are transferred into liquid media using working tools called probes.
They are used in laboratories, clinics, and in industrial research, and they perform diverse tasks
during sample preparation in Quality Assurance, scientific experiments, analyses, and in pilot or
short-series manufacture.
Examples of use:
• Cell disruption for paternity tests
• Cell disruption (extraction of microorganisms, tissue cells)
• Homogenising of liquids
• Emulsifying hard-to-mix liquids
• Dispersion of agglomerates
• Acceleration of chemical reactions
• Degassing of liquids
• Sample preparation in environmental analytics (wastewater tests, soil samples)
• Sonochemistry
1.3 CE conformity
SONOPULS ultrasonic homogenisers satisfy the CE-marking criteria in the European Union:
- 2014 / 35 / EU - Low Voltage Directive
- 2014 / 30 / EU - EMC Directive
- 2011/ 65 / EU - RoHS Directive
A declaration of conformity can be requested from the manufacturer by providing the serial number.

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1electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • [email protected]
1.4 Technical data
SONOPULS ultrasonic homogenisers are interference-free and CE-marked.
Safety: EN 61010-1, EMC: EN 61326-1
1.4.1 Ultrasound generator (GM)
Mains supply: 230 V~ (± 10 %) 50/60 Hz or 115 V~ (± 10 %) 50/60 Hz
Protection class: I
Ultrasonic frequency: 20 kHz ± 500 Hz
Frequency control/1: automatic, resonance frequency search
Time setting range: 0:00:01 – 9:59:59 [h:mm:ss] or continuous operation
Ultrasound operating mode: pulsating or continuous
Pulsation time ON (tE): 0.2 - 600 s - (see chapter 3.2.2)
Pulsation time OFF (tA): 0.3 - 600 s - (see chapter 3.2.2)
Ultrasound control: amplitude or power - (see chapter 3.2.4)
Amplitude setting range/2: 10 to 100 % in 1 % steps
Amplitude/power display: default and progress bars
Energy display: kJ
Temperature sensor: optional (TM 50 or TM 100)
Temperature display: -10 to +120°C
Data memory locations: 9
Operating elements: foil button
Operating data display: LC graphic display, illuminated
Remote operation (turning on/off): button on the ultrasonic converter, potential-free contact,
foot switch TS 8 (optional)
Interface: RS 232
Degree of protection: IP 30 pursuant to DIN EN 60529
Generator:
Generator GM 4200 GM 4400
Power/3, maximum 50/100/200 W 200/400 W
Ultrasonic frequency 20 kHz
Power setting range 30 - 150 W 60- 300 W
Weight (approx.) 3.2 kg 3.6 kg
External dimensions (L × W × H) 335 × 150 × 230 mm
/1 Frequency control:
The ultrasound generator has an automatic search feature for resonance frequency and rate adaptation while in operation. Causes for
a change in the resonance frequency are, for example, a warming of the ultrasonic converter and the probes, a change in the acoustic
load due to changes in viscosity, and even the mounting of another probe. A frequency drift during operation is corrected by the automatic
frequency control. The optimum working frequency is sought using the resonance frequency search, e.g. after a change in probe.
/2 The minimum and maximum amplitude setting range depends on the respective probe type. The valid amplitude setting range is stored
in the program.
/3 Power:
In the case of amplitude control, the ultrasonic power that is needed for the desired probe amplitude is dependent on the viscosity of
the medium. In order to prevent damage to the ultrasound generator and converter, the power is limited to the maximum permitted peak
value. In the case of highly-viscous media, this can result in the desired amplitude not always being reached.
The power depends on the connected ultrasonic converter.

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1.4.2 Ultrasonic converter (UW)
UW 50 UW 100 UW 200 UW 400
Start/Stop key: 
Stable at continuous
operation: 
Frequency: 20 kHz 20 kHz 20 kHz 20 kHz
Weight: 0.6 kg 1.5 kg 1.6 kg 1.7 kg
Dimensions: Ø 50 × 190 mm Ø 70 × 170 mm Ø 70 × 170 mm Ø 90 × 180 mm
Degree of protection: IP 20 IP 20 IP 20 IP 20
1.4.3 Other data
Ambient conditions according to DIN EN 61 010-1
Overvoltage category: II
Degree of contamination: 2
Permissible relative humidity up to 31 °C: 80 %
Permissible relative humidity up to 40 °C: 50 %
Permissible ambient temperature: 5 to 40 °C
Altitude: up to 2000 m above sea level
No dewing.
Only for indoor operation.

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1.4.4 Electromagnetic compatibility (EMC)
The device has been checked for electromagnetic compatibility (EMC) in accordance with
DIN EN 61326-1 and meets the requirements for Class B devices in accordance with EN 55011.
Suitable for use in institutions and other areas that are directly connected to a public low-voltage
power supply, e.g., laboratory facilities.
1.4.5 Remote operation
Several options are available for remote control and remote operation. Depending
on requirements, the most convenient solution may be selected.
Operating
element Methods Functions Connection
1Key manual Ultrasonic operation ON/OFF Fixed on theultrasonic
converter
2Foot switch foot-operated Ultrasonic operation ON/OFF Remote control connector
3 External control signal • Ultrasonic operation ON/OFF
• Status confirmation
Remote control connector
4 External data protocol • Status inquiry
• Full device control
RS 232 interface
a) Remote control connector
Contact assignment and functional description of the remote control connector
Diagram Contact Assignment Signal Function
1
2
3
45
1 Input 0V L
5V H
L Ultrasound operation ON
H Ultrasound operation
OFF
2 Output 0V L
5V H
L Ultrasound operation OFF
H Ultrasound operation ON
3 Earth
4 Output like contact 2
5 n.a.
Notes:
• The connection of power or voltage sources of any type is not permitted.
• The source resistance for the outputs is of 3.3 kΩ. The input resistance for subsequent signal
inputs, e.g. optocouplers, must be greater than or equal to 47 kΩ.
• The function of the signal input (contact 1) depends on the operating mode that is set for
the pulsation (see chapter 3.2.2). With the operating mode "by hand key", control is performed
statically, i.e. the ultrasonic operation is always activated under L status, and deactivated under
H status.
With all other operating modes, the input works as an edge-controlled changeover switch.
The input resistance totals approx. 50 kΩ.
• When connecting a changeover contact (toggle function) to connector 1, the make time
must be > 100 ms.