CAS BioMAT 1 User manual

BioMAT®1
Safety Cabinet

Section Description
Section 1
Your Manual
1.1
1.2
1.3
1.4
1.5
1.6
1.7
General Description
Quality Assurance/CE compatibility
Cabinet Siting
Installation
Avoiding Disturbances
Technical Data
Your Manual
Section 2
Using your Cabinet
2.1
2.2
2.3
2.4
2.5
2.6
2.7
2.8
Switches & Indicators
Start Up Procedure
Shut Down Procedure
Cleaning Procedure
Fumigation & Formalin Quantities
Fumigation Procedure
Airflow Measurements
Ultraviolet Radiation
Section 3
Cabinet Control Panel
3.1
3.2
3.3
3.4
3.5
Display
Alarm Circuits
Electrical Protection
Fan Speed Control
Engineers Menu
Section 4
Filter Replacement
4.1
4.2
4.3
Re-circ HEPA Filters
Exhaust HEPA Filter
General Notes
Section 6
Service & Spares
6.1
6.2
6.3
6.4
Service Schedule
Spares List
Drawings (attached)
Wiring Diagrams (attached)

General Description –1.1
Your BioMAT 1 Microbiological Safety Cabinet has been designed to provide optimum
performance for operator protection. Using the latest in microprocessor and fan technology, it
is designed to exceed the performance requirements of the European Microbiological Safety
Cabinet Standard EN12469:2000. The safety cabinet carcass and all seals are maintained under
negative pressure ensuring air cannot leak out during normal operation.
Class 1 Safety Cabinets offer a high level of operator protection; they ensure any aerosols
generated within the cabinet are filtered via high efficiency filtration (HEPA) prior to being
dispersed back into the laboratory or to atmosphere. Operator protection is provided by a
constant inflow of air through the working aperture of the front screen.
The Class 1 Microbiological Safety Cabinet can be supplied as standard in either of the
following two modes:
ØRe-circulating Type –Exhaust air from the safety cabinet is passed through two
high efficiency filters (HEPA) before being released back into the laboratory. The
exhaust air is made up of 100% of the total air volume handled by the safety
cabinet.
ØExhaust Type –Exhaust air from the safety cabinet is passed through a high
efficiency filter (HEPA) before being extracted to atmosphere through a fan
assisted extract system. The exhaust air is made up of 100% of the total air
volume handled by the safety cabinet.

Quality Assurance –1.2
Although fully tested before leaving our factory as part of the ISO 9001:2008 Quality Assurance
Programme, the specified performance will only be maintained if your cabinet is sited correctly
and regularly serviced. CAS can only accept responsibility for correct functioning of your
cabinet if: -
ØThe Safety Cabinet is correctly sited in the laboratory to avoid any adverse
conditions within the room that may affect the level of operator protection.
ØIt has been installed and commissioned by CAS trained personnel or approved CAS
agents.
ØExtension, modification, relocation, repairs or other maintenance is carried out by
CAS personnel or persons authorised by CAS or, in the case of electrical work, by
qualified electricians.
ØIn the case of repair or maintenance, replacement parts supplied by CAS must be
used.
ØThe electrical installation surrounding the unit and to which it is connected
comply with the latest IEC regulations.
ØThe unit is used and maintained in compliance with the instructions contained in this
manual.
CE Declaration of Conformity
CAS declares that the equipment supplied conforms to the following CE directives—
Machinery 2006/42/EC
Electro Magnetic Compatibility 2004/108/EC
Low Voltage 2006/95/EC

Cabinet Siting –1.3
The siting of your Safety Cabinet is extremely important. Air currents and the movement of
people in the laboratory can adversely affect the performance.
Safety Cabinets should be sited away from;
ØDoors and windows which open
ØDraughts caused by ventilation and air conditioning units
ØPedestrian traffic routes
ØOther safety cabinets or fume cupboards
ØAdjacent fridge & Incubator doors
These points are of particular relevance to class 2 safety cabinets. The diagram shown below
indicates some suggested locations for the correct siting of safety cabinets and highlights some
situations which should be avoided.
3 4
2
1
HORIZONTAL
LAMINAR FLOW
SUPPLY GRILLE
Position 1 - An acceptable site not affected by disruptive air currents
Position 2 - Well sited
Position 3 - Poorly sited if windows open –If not it should be a safe distance
from the cabinet opposite.
Position 4 - Poorly sited –can be affected by air currents from the opening door,
through traffic and the horizontal laminar flow workstation sited
directly opposite.

Installation –1.4
Safety Cabinets are sophisticated items of equipment containing delicate filters which require
expertise in their safe handling and installation into laboratories.
For exhaust type safety cabinets the exhaust ductwork route should ideally be surveyed and
ductwork installation be carried out by qualified engineers as it forms an integral part of the
system relating to the overall performance of the cabinet and is required to conform to various
safety standards.
A poorly installed cabinet may compromise the protection provided to both personnel and
work being handled and may present a hazard to other occupants of the building and the
public.
·Make-up Air
It is important that any make-up air compensating for the air exhausted from the safety
cabinet does not cause draughts to the discomfort of the laboratory staff or detriment of the
cabinet performance.
Air supply diffusers should be positioned more than 1500mm away from the front of the safety
cabinet and have a maximum velocity of no more than 0.30m/sec.
·Commissioning
When any safety cabinet is installed, it is necessary to carry out a number of commissioning
checks in order to ensure it is fully operational and that the performance on site satisfies the
current standard BS EN 12469:2000. This includes measuring the airflows, testing the HEPA
filters with a suitable challenge aerosol and a KI Discus Test (operator protection test) to assess
the containment of the cabinet.
CAS employs a team of fully trained installation and commission engineers to carry out all work
necessary. This ensures that all new safety cabinets operate to the desired performance.
·Site Surveys
If you have any queries regarding the siting of your safety cabinets we will be only too pleased
to arrange a site survey by one of our regionally based technical support staff.
·Periodic Maintenance & Servicing
To maintain safety cabinets at their optimum level of performance and to ensure lifetime
operation, regular servicing is necessary. CAS provides a full servicing and maintenance scheme
tailored to suit your individual needs. For more information on this please contact our service
department on 0161-655-8860.

Avoiding Disturbances –1.5
The sketches below show recommendations for avoiding disturbances for both cabinet
operator and safety cabinet performance.
1000
Keep pedestrians away from the front
of your safety cabinet Keep clear of adjacent wall
300
Position well away from the door openings
1500
1500
Position clear of bench opposite Keep clear of structural columns Keep well away from opposite wall
2000
Bench
C
300

Technical Data –1.6
Cabinet Size 900mm 1200mm 1500mm 1800mm
Dimensions
External Dimensions (w/d/h) 900/630/1250 1200/630/1250 1500/630/1350 1800/630/1350
Internal Dimensions (w/d/h) 895/625/700 1195/625/700 1495/625/700 1795/625/700
Opening to Work Area (w/h) 710/200 1010/200 1310/200 1610/200
Weight Typical Kg 150 200 250 300
Loading Capacity
Work Surface Kg 50 50 50 50
Air Volumes Typical
Exhaust Air Volume m³/sec 0.120 0.150 0.200 0.240
Re-circ Air Volume m³/sec 0.120 0.150 0.200 0.240
Pressure Drop Typical
Exhaust Clean Filters Pa 350 350 350 350
Exhaust Dirty Filters Pa 500 500 500 500
HEPA Filter Data
HEPA Type H14 (EN1822) H14 (EN1822) H14 (EN1822) H14 (EN1822)
Efficiency @ 0.3µ 99.999% 99.999% 99.999% 99.999%
Heat Gains Typical
Exhaust Watts 150 150 220 250
Re-circ Watts 150 150 220 250
Noise Typical
Noise Level dB (A) <58 <58 <58 <58
Light Typical
Lighting Level White Lux 900 900 900 900
Lighting Level St/St Lux 800 800 800 800
Duct Connection (Exh) Mm 160 160 200 200
Electrical Data Typical
Voltage Volts
AC
230 230 230 230
Frequency Hz 50 50 50 50
Phase N/A Single Single Single Single
Power Consumption kW 0.18 0.18 0.25 0.32
Current A 3 3 5 7
Internal Socket(s) 230v /13 Amp 230v /13 Amp 230v /13 Amp 230v /13 Amp

Your Manual –1.7
This user manual has been prepared to provide a basic operating and maintenance instruction.
It is intended to supplement existing in-house procedures and codes of practice, and not to
replace them. If further advice is required on the use or maintenance of this equipment, the
staff of Contained Air Solutions Ltd. will be pleased to assist wherever possible.
Switches & Indicators –2.1

The diagram below shows a typical control panel membrane layout for the BioMAT 1
Safety Cabinet.
COMPLIESWITHBS EN12469:2000
CLASS1MICROBIOLOGICALSAFETYCABINET
0
1 BioMAT1
BioMAT 1
AIRFLOW SAFE
DESCRIPTION LEGEND COLOUR FUNCTION
A –Cabinet Operation Green Starts / Stops power supply to
fans and control circuits.
B –Lights Yellow Controlling power to lights.
C –Gas Valve (Optional) Black
To activate solenoid Gas Valve.
Will only function when cabinet is
in safe working condition.
D –Alarm Mute Red
To mute audible alarm. Fault
indication on display will remain
until fault is rectified.
E –Fumigate Orange
To activate fumigation cycle. Will
only operate when cabinet is
switched OFF.
F –UV Lights (Optional) Blue
To activate power to UV Lights.
Will only operate when cabinet
and lights are switched OFF.
G –Key Switch
0
1
Grey Border
Key switch for supervisor control,
used to prevent cabinet operation
during fumigation.

Start Up Procedure –2.2
The following notes are for guidance where local laboratory instructions do not exist or are
inappropriate. They should complement, not replace, existing codes of practice issued by your
Laboratory Safety Officers.
ØEnsure power supply to the cabinet is switched on, as evidenced by the main display
being illuminated. The key switch on the front of the cabinet must be turned to position ‘1’=
(ON).
ØPress the green switch ‘A’on the control panel –this will energise the exhaust fan. The
main display will show `AIRFLOW STABILISING` for 60 seconds, once airflows are settled the
display will show `AIRFLOW SAFE`. On start up the audible alarm will sound until airflows are
safe; this may be muted using the Alarm Mute switch `D’.
NOTE: Should the airflows change significantly and fall out of the specifications laid
down in EN12469:2000 the cabinet alarms will be automatically activated.
ØSwitch on the interior lighting using switch ‘B’. Lighting can be activated when the
cabinet is OFF; this can be used if loading or un-loading the cabinet with equipment prior to
use.

Shut Down Procedure –2.3
ØThe work area should be cleared of any apparatus / equipment and cleaned in
accordance with laboratory codes of practice. The cabinet should be left running for a few
minutes to clear any residual aerosols.
ØSwitch off the interior light using switch `B`
ØSwitch off the cabinet using switch `A`.
ØPlace the closure panel / Night door over the working aperture.

Cleaning Procedure –2.4
Regular cleaning is very important to prevent the build-up of dirt and hence potentially
infectious material. Routine swabbing of work surfaces with 70% v/v IMS (ethanol) or IPA
(Isopropyl Alcohol) is recommended.
For cleaning the work surfaces, swabbing with a mild detergent in warm water is very
effective. Phenolic or Cresolic disinfectants should be avoided as they may stain the white
surfaces with a brownish colour. If they are used, any spillage should be quickly rinsed with
clean water and mopped up with an absorbent tissue. Most of the quaternary ammonium
compounds and the Glutaraldehyde based surface disinfectants are suitable.
To facilitate cleaning of the work zone and the interior, the whole front screen / visor may be
opened and lowered from the top, supported by the hinges at the lower edge. It is good
practice to clean the inside of the viewing screen to ensure adequate visibility of the working
zone. Always consult the Laboratory Safety Officer before carrying out this procedure.
Warning
If Hypochlorites are used to clean the stainless
steel interior of the safety cabinet they will
initially cause rust spots and over time may lead
to further damage.

Fumigation & Formalin Quantities –2.5
When handling hazardous materials the air space inside the cabinet should be decontaminated
regularly and always before servicing and following any spillages. Fumigation by formaldehyde
gas is the recommended decontamination procedure for biological hazards although there are
alternative methods available including VHP decontamination and Ozone decontamination.
To facilitate the fumigation a sequence has been incorporated in the cabinet controls, this is
detailed under fumigation procedure.
A convenient way of generating sufficient formaldehyde is to boil off Formalin (40%
formaldehyde BP or equivalent) in a suitable vessel such as a formalin vaporiser. These are
available from CAS.
Formalin Quantities
The recommended quantity stated in BS EN12469:2000 on Page 40 Annex J, Part 2 is 60ml
formaldehyde solution mixed with 60ml distilled water per cubic meter of cabinet volume.
However this quantity is now considered in excess of that required to achieve a satisfactory kill.
We have therefore produced the following table based on quantities employed by users of
large numbers of Safety Cabinets.
If you still consider that the quantities recommended in the British Standard are to be used,
you may find on completion of the sterilisation cycle that high quantities of fluids containing
formaldehyde are present in the cabinet.
Cabinet Size 900mm 1200mm 1500mm 1800mm
Formalin 15ml @40% 20ml @ 40% 25ml @ 40% 30ml @ 40%
Distilled Water 15ml 20ml 25ml 25ml
Always consult your Laboratory Safety Officer
prior to fumigation of a safety cabinet.
** If in doubt ask **

Fumigation Procedure –2.6
ØSwitch off the cabinet fans by pressing the green button ‘A’on the control panel.
ØFor re-circulating type safety cabinets the fumigation extract kit (optional) should be
fitted to the cabinet discharge and ensure that the manual damper is fully closed. For cabinets
connected to a duct system proceed as follows:
§Fill the formalin vaporiser with the correct amount of Formalin (see section 2.5 for
quantities) and screw on the aluminium cap –finger tight, having checked the gasket in the cap
is undamaged. If the vaporiser is free standing, place on the cabinet work tray, plug into
cabinet internal socket and switch on. Now fit the closure panel / night door, if using a
lightweight closure panel additional sealing tape will be required around the outside face to
ensure no leakage during fumigation.
§Now press the fumigation button `E’and the display will show `FUMIGATION IN
PROGRESS’. A countdown timer will be shown directly below in minutes e.g. `285 MINS`.
Warning
ØThe cabinet should be left for a minimum of 6 hours, preferably overnight.
Note: Once the ‘fumigate’button has been activated DO NOT SWITCH CABINET ON
until the cycle has been completed.
ØOn completion the cabinet will display `FUMIGATION COMPLETE` and `READY TO
VENT`. When ready, press the fumigation button to exit the cycle and then press the
green button `A` to purge the cabinet of formaldehyde gas.
Ensure all laboratory personnel are aware that
the fumigation is taking place; appropriate
warning notices should be put on all doors
entering the laboratory and on the cabinet being
fumigated.

For re-circulating type safety cabinets press the ‘fumigate’button to complete cycle and
open the manual shut-off damper on the fumigation adaptor kit (Optional) having first
fitted the extract tubing and placed the discharge point in a location approved by the
Laboratory Safety Officer.
** When fumigating a re-circulating type safety cabinet where venting is unobtainable,
a mobile carbon filter unit may be required. **
ØSwitch on the cabinet and then remove the sealing tape. To avoid formaldehyde being
drawn out of the cabinet remove the rubber bung when using a 100% sealing closure
panel.
ØWithin the first few minutes of purging, the majority of the formaldehyde gas within
the safety cabinet will be removed. However due to the fact that formaldehyde
adheres to the surfaces of the cabinet and within the media of HEPA filters, we
recommend that the cabinet be run for between 4 and 6 hours before the cabinet is
serviced or work re-commences.
ØRemove the (optional) fumigation kit from the cabinet discharge before
commencing to use the cabinet.
** Any poly-formaldehyde residue in the vaporiser may be removed by heating with water
containing a little mild detergent at neutral pH.
General Note
Other methods of generating formaldehyde and other methods of cabinet decontamination
can be employed; prior to using alternative methods your Laboratory Safety Officer should be
consulted. Contained Air Solutions will be pleased to advise if they are able, but a detailed
knowledge of every technique cannot be guaranteed.
If your cabinet is fitted with ports to allow fumigation using Vaporised Hydrogen
Peroxide (VHP) please refer to the VHP equipment manufacturer’s manual for the
correct application of this technique.

Airflow Measurements –2.7
It is recommended that the inflow airflow be checked regularly, i.e. weekly or monthly
according to cabinet usage. A rotating vane type anemometer with a head diameter of about
100mm is considered satisfactory. On 1200mm wide cabinets, take 5 readings, namely one in
each corner and one in the centre on the work area aperture. Readings should be not less than
0.70m/sec or greater than 1.0m/sec. Readings above 1.0m/sec may cause problems with
containment, this may be checked by conducting a KI test. Anemometer positions for the
1200, 1500 & 1800mm size of cabinets are illustrated below. The results should be recorded to
monitor cabinet performance over time.
1200mm BioMAT 1
1500 & 1800mm BioMAT 1

Ultraviolet Radiation (Optional) –2.8
Ultraviolet Radiation (UV) lamps may be fitted as an optional extra; these can be fitted as new in
our factory or retrofitted at a later date on site. If installed the cabinet will have1 or 2 (Depending
on cabinet size) short wavelength Ultraviolet (UV) tubes emitting 254 nano metres fitted to the
inside work area of the safety cabinet.
As a safety feature the UV tubes are interlinked with the cabinet lights to prevent them being used
when the cabinet is in use.
Over time, the effective life of UV tubes is known to reduce; therefore we would recommend UV
tubes are replaced on an annual basis to ensure maximum efficiency.
Applications
Many bacteria are quite resistant to UV Radiation, and may require prolonged exposure for
sterilisation. Dry and/or protein covered organisms may be protected against UV and may be only
slightly affected if at all. However, moist, vegetative cells without too much protein covering are
killed with reasonable effectiveness after 3-4 hours exposure.
Warning
Extreme care must be exercised when using UV
radiation. Consult your Laboratory Safety Officer
prior to use.
UV Radiation can cause burns to unprotected skin
and it is very important not to look directly at the
illuminated tubes with the naked eye.

Display - 3.1
The cabinet display is located in the centre of the control membrane; it incorporates
backlighting to ensure all signs can be clearly seen from the operating position.
Typical display during normal operation shown below:
AIRFLOW SAFE
BioMAT 1
In the event of an alarm condition the display will clearly indicate the fault; this will remain
displayed until fault is rectified.
Typical display during alarm condition shown below:
LOW AIRFLOW
Should the cabinet produce an alarm condition it may be necessary to arrange for a Service
Engineer to attend site, in such cases please contact our Service Department on 0161-655-
6183.

Alarm Circuits –3.2
There are three standard alarm circuits on the BioMAT 1 Re-circulating and Exhaust type Safety
Cabinets.
1. INFLOW LOW ALARM
Sensed by an accurate pressure sensor mounted on the main printed circuit board and
connected via tubing to the sensing points within the cabinet body, this will sense low airflow
caused by fan failure or filter soiling, and transmit the signal to the display meter on the front
panel and alarm circuit. In the event the alarm is triggered the display will show `LOW
AIRFLOW`, and the audible alarm will sound, this can be muted using the alarm mute button.
2. INFLOW HIGH ALARM
Sensed by an accurate pressure sensor mounted on the main printed circuit board and
connected via tubing to the sensing points within the cabinet body, this will sense high airflow
and transmit the signal to the display meter on the front panel and alarm circuit. In the event
the alarm is triggered the display will show `HIGH AIRFLOW`, and the audible alarm will sound,
this can be muted using the alarm mute button.
3. FRONT SCREEN
Front screen position is monitored by a micro-switch fitted to the front screen
assembly. When opened the display will show `VISOR OPEN’and an audible alarm will sound.
This can be muted using the alarm mute button.
** The alarm circuit is activated each time the cabinet is switched on.
It is important to ensure that the pressure tubing is not damaged or split where it is connected
to the pressure sensor. The pressure sensor, in the event of inadequate airflow in either part
of the system, will cause the alarm circuit to be activated with a flashing red indicator on the
alarm mute button, an intermittent audible alarm tone, and the alarm system will clearly show
on the display the fault. The audible alarm tone can be silenced by depressing the ‘alarm
mute’button on the control panel after which the red light will remain continuously lit until
the correct airflow is reinstated or the cabinet is switched off.