Cellink BIO X User manual

BIO X Thermoplasc Printhead
User Manual

Contents
1.Package contents 1
2.Technical specicaons 3
3.Safety informaon 7
3.1 BIO X system warnings 8
3.2 Thermoplasc Printhead warnings 8
4.Geng started 11
4.1 Unpacking and installaon 12
4.2 Heang the Thermoplasc Printhead 15
4.3 Recommendaon: Using external air supply 17
4.4 Loading the granulate 20
4.5 Your rst bioprint 24
4.6 Relling 31
5.Cleaning 33
6.Prinng opmizaon 37
7.Relevant G-code commands 41
8.Frequently asked quesons 45
9.Maintenance 51
9.1 Post-prinng instrucons 52
9.2 Long-term maintenance 52
Appendix A: Consumables 53
Support informaon 55

1
Package contents
01

2
01 Package contents
Item Part number Quanty
Thermoplasc Printhead 000000020347 1
Metal cartridge 000000020358 1
4-mm tube connector M3 (straight stainless) 000000010160 1
Thermoplasc Printhead top cap 000000020363 1
Locking ring 000000020361 1
Plunger 000000020362 1
Plunger removal tool (160 mm) 000000020373 1
Blue tubing (150 mm) 000000010109 1
Wrench 000000020537 1

3
Technical specicaons
02

4
02 Technical specicaons
• Compable with CELLINK thermoplasc cartridge.
• Dimensions (height x width x depth):
• With cartridge (no tubing or nozzle): 165 x 32.5 x 44 mm.
• Without cartridge or nozzle: 129.5 x 32.5 x 44 mm.
• Weight: 260 g.
• Maximum recommended pressure: 700 kPa. We recommend using an external pump with
the Thermoplasc Printhead.
• Material composion:
• External surfaces: Stainless steel.
• Internal surfaces (metal cartridge): Stainless steel.
• Insulators: Polyether ether ketone (PEEK).
• Recommended operang temperature: 20-23 degrees Celsius.
• Running temperature (in recommended condions): 26-32 degrees.
• Heang capacity and rate:
• Total heang range: 30-250 degrees.
• Heang rate: Room temperature-250 degrees (15 minutes).
• Cooling capacity: N/A.
• Build volume (X, Y, Z): 128 x 85 x (37–A) mm (A = p length).

5
02 Technical specicaons
Figure 1: Theorecal maximum build volume shown from the top and side view using the Thermoplasc Printhead (assuming a nozzle
length of 7 mm). Exact build volume depends on the cartridge’s posion in the printhead and the nozzle/needle used. Build volume may
be lower when combining the Thermoplasc Printhead with a printhead that restricts printbox movement. Please consult the printhead
manual for the printheads being used for specic limitaons.

6
02 Technical specicaons
Table 1: Theorecal build volume based on common p lengths. Actual volume may vary based on build plate’s thick-
ness, size, shape and level, as well as on cartridge posion and ghtness of the nozzle. X, Y and Z are measured from
the front le corner of the printbed.
Tip Type Tip Length Nozzle/Needle Length X Y ZBuild Volume
Metal M6 7mm 7mm 12.8 cm 8.5 cm 3.7cm 402 cm³

7
Safety informaon
03

8
03 Safety informaon
3.1 BIO X system warnings
• Please consult the BIO X manual for BIO X-specic warnings and safety procedures.
3.2 Thermoplasc Printhead warnings
• Clean the printhead using instrucons in the Maintenance secon. Do not clean the
printhead by submerging it in liquid or using excessive spraying. Liquid inside the printhead
can damage the circuitry and motor.
• The Thermoplasc Printhead operates at very high temperatures. The LED on the printhead
will turn yellow to indicate a warm printhead and red to indicate a hot printhead. Do not
touch the printhead when the LED is red. Touching a hot printhead risks serious injury.
• Only load the cartridge with dry thermoplasc granulate. Ensure the cartridge is clean
and dry before loading the granulate. Only use the cartridge with CELLINK-supplied
thermoplasc granulates or granulates from our recommended suppliers.
• Load and unload the Thermoplasc Printhead onto the BIO X printhead mounts using
guidelines in the Geng Started secon. Do not pull or push the printhead with excessive
force while it’s loaded on the BIO X. Using excessive force risks damaging the motor and its
guides.

9
03 Safety informaon
• DO NOT use a jerking moon to remove the printhead. Using a jerking moon risks hing
and damaging the HEPA lter
• DO NOT touch a moving printhead. Fingers can be crushed between the printhead base and
motor arm and can result in serious injury.

10

11
Geng started
04

12
04 Geng started
NOTE: The prinng parameters displayed on this manual might not be accurate for your specic protocol. For recommended
parameters please consult the documentaon for the bioink being used.
4.1 Unpacking and installaon
1. Open the package. Remove the pre-assembled Thermoplasc Printhead.
2. Check for all components of the Thermoplasc Printhead and accessories (Figure 2).
Figure 2: Thermoplasc Printhead com-
ponents. These include: (A) Thermoplasc
Printhead body, (B) the stainless-steel car-
tridge with nozzle, (C) the top cap, (D) the
insulated locking ring, (E) the stainless steel
plunger, (F) the plunger removal tool and (G)
the wrench for ghtening the cartridge and
the nozzle.
AB
C
D
E
FG

13
04 Geng started
3. Assemble the Thermoplasc Printhead (Figure 3).
Figure 3: Thermoplasc Printhead assembly.
A
BC
• Place the plunger inside the metal cartridge. Aach the
top cap. (Plunger use is oponal.)
• Insert the assembled cartridge into the Thermoplasc
Printhead (Figure 3A). Twist unl the cartridge is screwed
into place. Use the wrench to ghten (Figure 3B).
• Posion the insulated locking ring downward to secure
the assembled cartridge to the Thermoplasc Printhead
(Figure 3C).

14
04 Geng started
4. Aach the Thermoplasc Printhead to
the BIO X printbox. Align the printhead
above the desired printhead mount and
push downwards using one hand (Figure
4A). Secure the boom of the printhead
mount with your opposite hand to prevent
straining the motor (Figure 4B).
Figure 4: Aaching the Thermoplasc Printhead to the BIO X. (A) Line up the printhead with
a mount on the printbox. (B) Slide the printhead down with one hand and secure the boom
of the printhead mount with the other hand. Once aached, the lights on the printhead will
turn from white to blue.
AB

15
04 Geng started
4.2 Heang the Thermoplasc Printhead
NOTE: The steps under this secon will test the temperature funconality of your Thermoplasc Printhead. It is only necessary to follow
these steps once, aer receiving your new printhead.
1. Assemble and aach the Thermoplasc Printhead (Secon 4.1).
2. Go to the Ulies Menu and then the Tools submenu.
3. Set the printhead temperature (Figure 5).
Figure 5: Preseng temperature for the
Thermoplasc Printhead.

16
04 Geng started
4. Set the temperature of the printhead to 150 degrees Celsius.
5. Allow the printhead to heat up to 150 degrees.
WARNING: Do not touch the printhead while heang. Doing so risks serious injury.
6. Once the printhead reaches 150 degrees, set the temperature to 250 degrees.
7. Once it reaches 250 degrees (± 5 degrees), turn o the printhead temperature and allow it
to cool down to room temperature.
NOTE: A faint burning smell can be caused by dust and other residue and is not necessarily a
cause for concern.
8. Aer cooling, remove the printhead from the printhead mount.