Cellink BIO X User manual

BIO X Thermoplasc Printhead
User Manual

Contents
1.Package contents 1
2.Technical specicaons 3
3.Safety informaon 7
3.1 BIO X system warnings 8
3.2 Thermoplasc Printhead warnings 8
4.Geng started 11
4.1 Unpacking and installaon 12
4.2 Heang the Thermoplasc Printhead 15
4.3 Recommendaon: Using external air supply 17
4.4 Loading the granulate 20
4.5 Your rst bioprint 24
4.6 Relling 31
5.Cleaning 33
6.Prinng opmizaon 37
7.Relevant G-code commands 41
8.Frequently asked quesons 45
9.Maintenance 51
9.1 Post-prinng instrucons 52
9.2 Long-term maintenance 52
Appendix A: Consumables 53
Support informaon 55

1
Package contents
01

2
01 Package contents
Item Part number Quanty
Thermoplasc Printhead 000000020347 1
Metal cartridge 000000020358 1
4-mm tube connector M3 (straight stainless) 000000010160 1
Thermoplasc Printhead top cap 000000020363 1
Locking ring 000000020361 1
Plunger 000000020362 1
Plunger removal tool (160 mm) 000000020373 1
Blue tubing (150 mm) 000000010109 1
Wrench 000000020537 1

3
Technical specicaons
02

4
02 Technical specicaons
• Compable with CELLINK thermoplasc cartridge.
• Dimensions (height x width x depth):
• With cartridge (no tubing or nozzle): 165 x 32.5 x 44 mm.
• Without cartridge or nozzle: 129.5 x 32.5 x 44 mm.
• Weight: 260 g.
• Maximum recommended pressure: 700 kPa. We recommend using an external pump with
the Thermoplasc Printhead.
• Material composion:
• External surfaces: Stainless steel.
• Internal surfaces (metal cartridge): Stainless steel.
• Insulators: Polyether ether ketone (PEEK).
• Recommended operang temperature: 20-23 degrees Celsius.
• Running temperature (in recommended condions): 26-32 degrees.
• Heang capacity and rate:
• Total heang range: 30-250 degrees.
• Heang rate: Room temperature-250 degrees (15 minutes).
• Cooling capacity: N/A.
• Build volume (X, Y, Z): 128 x 85 x (37–A) mm (A = p length).

5
02 Technical specicaons
Figure 1: Theorecal maximum build volume shown from the top and side view using the Thermoplasc Printhead (assuming a nozzle
length of 7 mm). Exact build volume depends on the cartridge’s posion in the printhead and the nozzle/needle used. Build volume may
be lower when combining the Thermoplasc Printhead with a printhead that restricts printbox movement. Please consult the printhead
manual for the printheads being used for specic limitaons.

6
02 Technical specicaons
Table 1: Theorecal build volume based on common p lengths. Actual volume may vary based on build plate’s thick-
ness, size, shape and level, as well as on cartridge posion and ghtness of the nozzle. X, Y and Z are measured from
the front le corner of the printbed.
Tip Type Tip Length Nozzle/Needle Length X Y ZBuild Volume
Metal M6 7mm 7mm 12.8 cm 8.5 cm 3.7cm 402 cm³