Cellink BIO X6 User manual
BIO X6 User Manual v. 1.0 1
BIO X6
UserManual
BIO X6 User Manual v. 1.0 2
Welcome to the world of bioprinting!
Not too long ago, the idea of creating living human tissue was no more than a dream, but not
anymore. The technology in front of you is the next step on the path to regenerative medicine
and tissue engineering.
printhead platform and a more intuitive bioprinting process, you can focus on your research
and dream bigger than ever before.
Featuring unparalleled usability and versatility, the BIO X6 will take your research to the next
chamber sterilization, a temperature-controlled printbed, an improved, precise, and fast
touchless autocalibration system and a user-friendly interface, the BIO X6 is ready to
overcome the most complex bioprinting challenges.
This manual is your guide to getting started with the BIO X6. Read the manual in its entirety
before using the BIO X6.
Because we regularly update our software and release updated bioprinting protocols for
these added features, please refer to the digital manual included with your tablet for the
latest protocols and printing instructions.
Create the future of medicine!
BIO X6 User Manual v. 1.0 3
Contents
1Safety information 9
1.1 General safety information 10
1.2 Unpacking, lifting, and carrying 11
1.3 Electrical information 11
1.4 Protective equipment 11
1.5 Hazardous materials 12
1.6 Electrostatic discharge (ESD) protection 12
1.6.1 Discharging 12
1.6.2 Clothing 12
1.7 Compliance 13
2Specifications 15
2.1 Product overview 15
2.2 Technical specifications 17
3Getting started 20
3.1 Unpacking the BIO X6 20
3.2 Contents of the box 22
3.3 Setting up the BIO X6 24
3.4 Starting the BIO X6 for the first time 25
3.5 Calibration trimming 27
4Bioprinting with the BIO X6 30
4.1 Mounting of bioprinting equipment 30
4.1.1 Printheads 30
4.1.2 Printbed 32
4.2 Sterilizing the printing chamber 33
4.3 Printing utilities menu 34
4.3.1 Connect tab 35
4.3.2 Printer tab 36
4.3.3 Printhead Configuration tab 38
4.3.4 Move tab 40
4.3.5 Machine calibration tab 40
4.3.6 Camera tab 42
BIO X6 User Manual v. 1.0 4
4.3.7 Photocrosslinking tab 43
4.3.8 Update Printer tab 44
4.3.9 About tab 45
4.4 Bioprinting from a 3D model 46
4.4.1 Print surface 47
4.4.2 Model 49
4.4.3 Layer settings 52
4.4.4 Printhead settings 56
4.4.5 Printer settings 58
4.4.6 Summary 60
4.4.7 Calibration and Automatic Bed Levelling (ABL) 62
4.4.8 Automatic calibration 63
4.4.9 Manual calibration 66
4.4.10 Printing model 71
4.4.11 Manual photocrosslinking Model 74
4.5 Bioprinting droplets 75
4.5.1 Print surface 76
4.5.2 Droplet 77
4.5.3 Printhead settings 78
4.5.4 Printer settings 80
4.5.5 Summary 82
4.5.6 Calibration and Auto Bed Levelling (ABL) 84
4.5.7 Printing droplets with multiple heads 87
4.5.8 Manual photocrosslinking Droplet 90
4.6 BIO X6 Protocol Parameter Guidelines 91
4.6.1 BIO X6 Protocol Parameter Guidelines Protocol aim 91
4.6.2 Background 91
4.6.3 Parameters in DNA Studio 91
4.6.4 Helpful hints and troubleshooting 92
5Gcode Editor 95
5.1 Launching the Gcode Editor 96
5.2 Gcode Editor menu options 97
5.3 View options 99
5.4 Find and replace 100
BIO X6 User Manual v. 1.0 5
5.5 Gcode preview mode 101
5.6 Gcode Syntax description 103
6Draw & Print 106
6.1 Surface 107
6.2 Draw 108
6.2.1 Layers panel 108
6.2.2 Tool bar 110
6.2.3 Shape Parameters bar 112
6.2.4 Viewport options 114
6.2.5 Actions bar 115
6.2.6 Gcode generation 116
7Shape generator 118
7.1 Create new model 118
7.2 Generate stl file and transform model 120
8Print report 123
8.1 Prerequisites 123
8.2 Opening and adding comments to print report 124
8.3 Information included in the print report 125
8.4 Opening previous print reports 129
9Coaxial printing 131
9.1 Coaxial set-up 131
9.2 Equipment for coaxial printing 132
10 Maintenance 135
10.1 Maintenance plan 135
10.2 Troubleshooting 136
10.2.1 Software update 136
10.2.2 Troubleshooting quick guide 138
10.3 Manually cleaning or sterilizing the BIO X6 143
11 Terms and conditions 147
11.1 General terms and conditions 147
11.1.1 Orders and supplies etc. 147
11.1.2 Payment 148
11.1.3 Service 148
11.1.4 General conditions of delivery 148
BIO X6 User Manual v. 1.0 6
11.1.5 Product liability 149
11.1.6 Acceptance 150
11.1.7 Liability for defects 150
11.1.8 Software 151
11.1.9 Granted rights 151
11.1.10 Trademarks and other intellectual property rights 152
11.1.11 Permitted use of the products 152
11.1.12 Limitation of liability 152
11.1.13 Force majeure 153
11.1.14 Confidentiality 153
11.1.15 Miscellaneous 154
11.2 End-user license agreement BIO X6 155
11.2.1 License 155
11.2.2 Authorized users 156
11.2.3 Reservation of rights 156
11.2.4 Updates or revisions 156
11.2.5 Trademarks 156
11.2.6 Third-party materials 157
11.2.7 Digital content and data 157
11.2.8 Limitation of liability 158
11.2.9 Restrictions and disclaimers specific to 3D printers 159
11.2.10 Indemnity 160
11.2.11 Term, termination, and survival 160
11.2.12 Export control 160
11.2.13 Miscellaneous 161
11.3 Warranty 163
12 Printhead manuals 167
12.1 CELLINK Standard Pneumatic Printhead 167
12.1.1 Getting started 167
12.1.2 Printhead overview 168
12.1.3 Mounting a cartridge in the printhead 169
12.2 CELLINK Temperature-controlled Printhead 170
12.2.1 Getting started 170
12.2.2 Printhead overview 171
BIO X6 User Manual v. 1.0 8
01
Safety
BIO X6 User Manual v. 1.0 9
1 Safety information
Review the entire manual, as well as the manuals for any accessories, before
using the BIO X6. Mishandling can lead to equipment damage and severe
injury. The following symbols are used to indicate risk of equipment damage or
personal injury:
This symbol indicates risk of injury or equipment damage.
This symbol indicates a heated surface. The BIO X6 has heated surfaces that
may cause personal injury or equipment damage if not treated with care. A
physical symbol labels these surfaces on the BIO X6, and the symbol appears
on the BIO X6 touchscreen when the print chamber is heated.
This symbol indicates the presence of UV light. Keep the door closed and wear
proper safety attire during UV sterilization and UV curing. This symbol appears
on the screen when a UV light source is active.
Manufacturer
Cellink Bioprinting AB
Långfilsgatan 7
412 77 Gothenburg Sweden
BIO X6 User Manual v. 1.0 10
1.1 General safety information
If the BIO X6 acts in a way that is not described in this manual, turn it off and contact
CELLINK. Use the BIO X6 for its intended purposes only. Do not modify its instruments,
subcomponents, or accessories. Do not open or disassemble the BIO X6 or attempt any
service other than those described in the manual.
Never place your fingers or any part of your body near BIO X6 until all parts have
stopped moving. Moving parts can cause injury, (BIO X6 may start moving
without warning).
Never clean or service the BIO X6 while it is on. Always turn off the power and
disconnect any external sources of power and air pressure before cleaning or
servicing the BIO X6 in all cases (including changing the filter).
The BIO X6 uses UV light for sterilization and curing. Never look directly at UV
light. Never expose skin to UV light. Eye or skin exposure can result in serious
personal injury. Always keep the BIO X6 door closed during the Clean
Chamber mode and photocuring operation. (The UV modules might be hot
right after use.)
The BIO X6 has heated surfaces that can reach temperatures of up to 250ºC.
Never touch heated surfaces when using the heating function. Always let
printheads and printbed cool down after using the heating function.
Always ensure that the equipment is correctly mounted before use. Improperly
mounted printheads, cartridges, cables, liquid spouts, and air spouts can be
dangerous. If any equipment appears damaged, turn off the BIO X6, unplug all
external connections, and contact CELLINK before further use.
Never tamper with any safety interlocks. Tampering risks equipment damage
and personal injury.
BIO X6 User Manual v. 1.0 11
1.2 Unpacking, lifting, and carrying
Read how to safely unpack and setup the BIO X6 in Section 3 of this manual.
The BIO X6 is heavy (approximately 50 kg/110 lbs.). Never attempt to lift alone.
Always use a minimum of two people to unpack and move the machine.
1.3 Electrical information
Always connect the BIO X6 to a grounded socket and use the voltage described
in the specifications. Improper electrical handling can cause equipment damage
and personal injury.
1.4 Protective equipment
Always wear gloves and eye protection during bioprinting, UV curing and
sterilization.
Always wear gloves and eye protection while handling hazardous materials,
including materials that are toxic, corrosive, and carcinogenic.
Read the material safety data sheets, packing labels, and the manufacturers or
product.
BIO X6 User Manual v. 1.0 12
1.5 Hazardous materials
Consult material safety data sheets, packaging labels, and the manufacturers or
flammable and corrosive materials. Ensure all operators are informed of the
characteristics of the material, as well as its compatibility with the dispensing
unit. Always use appropriate safety equipment and attire. If you are unsure of the
compatibility of a material, contact CELLINK.
After using ethanol to clean the BIO X6, wait at least 60 seconds before
bioprinting to ensure that all flammable vapors have been ventilated.
1.6 Electrostatic discharge (ESD) protection
The BIO X6 print chamber is ESD sensitive. Follow the instructions below to ensure that you
do not damage the BIO X6 or its accessories.
1.6.1 Discharging
Always discharge yourself before using the BIO X6 print chamber and handling printheads.
Always discharge yourself before touching any ESD sensitive parts.
To discharge yourself, touch a grounded metal surface. Examples of grounded metal
surfaces include:
Any metal surface in a laminar flow hood
Any metal sinks
The frame of the BIO X6 when connected to a grounded power outlet, as well as any
exposed screws, including the screws on the left-side door hinges
1.6.2 Clothing
Avoid charging yourself to ensure ESD protection. Wear clothes made from cotton when
appropriate.
BIO X6 User Manual v. 1.0 13
1.7 Compliance
By using the BIO X6, you agree to the terms stated here and, in the warranty (see Warranty).
Except for the express warranty set forth in the warranty information and in our software,
Except for any warranty, condition, representation, or term the extent to which cannot be
excluded or limited by applicable law, CELLINK and its suppliers make no warranty,
condition, representation, or term (expressed or implied, whether by statute, common law,
custom, usage or otherwise) as to any matter including, without limitation, noninfringement of
third-party rights, merchantability, integration, or fitness for a particular purpose.
You assume responsibility for selecting the solution to achieve your intended results, and for
the installation of, use of, and results obtained from the equipment and software. Without
limiting the foregoing provisions, CELLINK makes no warranty that the equipment or
software will be error-free or free from interruptions or other failures or that the software or
equipment will meet your requirements.
Under no circumstances and under no legal theory, whether in tort, contract, or otherwise,
shall CELLINK or its suppliers be liable to purchaser or to any other person for loss of profits,
loss of goodwill, or any indirect, special, incidental, or consequential damages, or damages
for gross negligence of any character.
Under no circumstances can CELLINK be responsible for consequences of misuse of the
instrument or for use of the instrument outside the use specified in this user manual, be they
material or personal.
BIO X6 User Manual v. 1.0 14
02
Specifications
BIO X6 User Manual v. 1.0 15
2 Specifications
2.1 Product overview
Figure 1 Front view of BIO X6
1. Clean Chamber unit
2. Printbox (printhead mount 1-6)
3. Photocuring LED 1-4
4. Printbed
5. UV protective door
6. Docking station
BIO X6 User Manual v. 1.0 16
Figure 2 Back view of BIO X6
1. Power switch
2. Fuse
3. Power socket
4. Pneumatic port
5. USB port
6. Ethernet port
BIO X6 User Manual v. 1.0 17
2.2 Technical specifications
Electrical
Power input: 100240 VAC, 5060 Hz
Power (max): 600 W
Current (max): 6 A
Fuse: 250 VAC, F6.3A
Outer dimensions
Length: 850 mm (33.46 in)
Width: 400 mm (15.75 in)
Height: 500 mm (19.69 in)
Net weight: 47.4 kg (104.5lb)
Build volume (L x W x H): 128 x 90 x 85 mm
Filter class: HEPA 14
UV-sterilization: UV-C (275 nm) 2W output
UV-curing modules: 365 nm, 405 nm, 475 nm, 520 nm
No. of printhead slots: 6
Calibration options: X-Y-Z axis, Manual (mechanic based)
X-Y-Z axis, Automatic (touchless based)
Printbed, automatic bed levelling ABL (mechanic
based)
Resolution
Theoretical resolution XY: 1
Theoretical layer resolution Z:
Software Resolution XYZ:
BIO X6 User Manual v. 1.0 18
Pressure
Pressure range (internal pump): 0200 kPa
Pressure range (external air supply): 0700 kPa
Output Pressure Offset: +/- 5 kPa
Temperature
Printbed temperature range: 465°C (lowest temperature 17°C below chamber
temperature)
Printhead temperature range: 4250°C (dependent on printhead type)
Pneumatic printhead: 3065°C
Temperature-controlled Printhead: 465°C (lowest temperature 17°C below chamber
temperature)
Thermoplastic Printhead: 50250°C
Supported file formats: G-code, stl, amf, 3mf, obj.
User interface: Desktop application DNA Studio
Connectivity: USB Storage, Ethernet connection
BIO X6 User Manual v. 1.0 19
03
Getting started
BIO X6 User Manual v. 1.0 20
3 Getting started
3.1 Unpacking the BIO X6
Do not attempt to unpack the BIO X6 without reviewing the procedures below.
Doing so can lead to personal injury and equipment damage.
Always lift the BIO X6 from the underside.
The BIO X6 is heavy (approximately 50 kg/110 lbs). Never attempt to lift it alone.
Always use a minimum of two people to unpack and move the BIO X6.
Follow these steps to unpack the BIO X6.
1. Clear a sturdy table and a floor space of at least 2 by 3 meters.
2. Place the box on the floor according to the printed arrows.
3. Remove the pallet straps.
4. Carefully remove the top box.
Figure 3 Direction for unpacking and
transportation
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