Cellink INKREDIBLE User manual

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INKREdIBLE
User manual
CELLINK AB
Arvid Wallgrens Backe 20
Gothenburg, SE 41346
SWEDEN

Hello and welcome to the excing world of
3D Bioprinng!
We are excited to have you here. The technology
you have in front of you has the potenal of creang
real, live human ssues and it is only your imaginaon
that limits what you can do. This manual will serve
as your guide for seng up your INKREDIBLE 3D
Bioprinter, maintaining it, and also nofying you
about safety precauons to ensure a long-lasng
product lifeme. Make sure you read the enre
manual before using the product for the rst me.
Let`s begin this excing journey!

1. Safety informaon 4
1.1 General safety informaon 6
1.2 Protecve equipment 7
1.3 Liquids and dispensing media 7
1.4 Opening the product and service 7
2. Introducon to your INKREDIBLE 3D Bioprinter 8
2.1 Specicaons 10
3. Unpacking your INKREDIBLE 3D Bioprinter 12
3.1 Contents of the box 15
4. Product map 18
5. Operang your INKREDIBLE 3D Bioprinter 20
5.1 Seng up for rst me use 22
5.2 Starng a print 23
5.3 Advanced features 26
6. How to install and setup HeartWare 28
6.1 Installing Heartware (PC) 30
6.2 Installing Repeer-Host (Mac) 34
6.3 Seng up HeartWare 39
7. Slicing with HeartWare 42
7.1 What is Slicing? 44
7.2 HeartWare interface 45
7.3 Basic slicing workow 49
8. Maintenance and support 54
8.1 Maintaining your INKREDIBLE 3D Bioprinter 56
8.2 Support for your INKREDIBLE 3D Bioprinter 58
9. Warranty and guarantee 62
Contents

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1. Safety informaon

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WARNING! This symbol will indicate if there is risk of personal injury.
CAUTION! This symbol will indicate if there is risk of causing damage to your
INKREDIBLE 3D Bioprinter or other equipment.
Before you start using your INKREDIBLE 3D bioprinter, please take me to review the
enre manual. Maloperaon of the INKREDIBLE 3D bioprinter instrument can lead to
severe and irreversible injuries if the safety instrucons are not followed. Throughout the
manual we will use the following two symbols to indicate if there is risk for equipment
damage or personal injury:
In order to respond to the INKREDIBLE 3D Bioprinter and its relevant security
requirements, the instrument has to be operated by trained personnel, who are aware
of the risks and dangers that can be induced to themselves, as well as for the sta
being in close proximity with the INKREDIBLE 3D Bioprinter. The sta being involved
in the installaon, or the maintenance of the system or a part of the system, has to be
qualied through appropriate training. Use the instrument only for its intended purpose
as described in the documentaon. Do not modify the instrument, sub-components or
accessories.
1.1 General safety informaon
WARNING! Never reach into the instrument unl all components of the instrument
have come to a complete stop. Moving parts can cause serious injuries.
WARNING! Before cleaning, inspecng, adjusng, repairing or disassembling the
instrument or the dispensing units, stop the instrument, turn o the main switch
and disconnect if from its electrical power source.
WARNING! The INKREDIBLE 3D Bioprinter is ed with a 365nm (405nm
oponal) UV LED. Do not look directly at the light source. Always wear protecve
gear when operang the system, including UV protecve goggles.
WARNING! Improper mounted pressurized air spouts, liquid spouts or cartridges
can be dangerous. Verify that all connecons are properly connected and all
components are specied for use with the intended pressure. Verify that the
supplied air pressure does not exceed 400 kPa.
In addion to the manual, we are connously uploading instrucon videos on our
YouTube-channel, CELLINK3D. To access it go to:
hp://ny.cc/rbz7xy

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1.2 Protecve equipment
WARNING! Always wear protecve goggles and gloves while handling dangerous
materials that can be absorbed by skin or which are toxic, strike-aracve,
corrosive, causing allergies, carcinogenic, reproducon-endangering or mutagenic
to humans.
WARNING! Always read the material safety data sheets, packing labels,
manufacturer or dealer’s catalogue rst and use adequate safety equipment.
1.3 Liquids and dispensing media
WARNING! Before starng to work with hazardous dispensing materials,
the operator has to be informed about the characteriscs of the material and
its compability with the dispensing unit. The informaon can be found in the
material safety data sheets, packaging labels, manufacturer or dealer catalogues.
Always use adequate safety equipment.
1.4 Opening the product and service
WARNING! The INKREDIBLE 3D Bioprinter SHOULD NOT be taken apart. Doing
so endangers both the users and the equipment! The INKREDIBLE 3D Bioprinter
IS NOT user servicable! Opening of the instrument’s electrical panel will void the
warranty.
WARNING! Always wear protecve goggles and gloves, and secure the environment
around instrument from other personnel while operang the INKREDIBLE 3D
bioprinter. All safety equipment, including UV protecve safety goggles, must be
used when operang the UV curing system.

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2. Introducon to your INKREDIBLE
3D Bioprinter

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The INKREDIBLE 3D bioprinter is a pneumac-based extrusion bioprinter with dual
printheads and UV LED curing system for bioprinng of complex human ssue models
and organs for ssue engineering research. It is a cost-eecve unit for innovators
to enter the 3D bioprinng eld and start bioprinng living ssues at ease. It can be
used as a standalone unit thanks to its LCD controller or it can be monitored through
a computer with the use of the accompanied soware. Your three-dimensional CAD
models are translated into coordinates and instrucons for the INKREDIBLE to allow the
bioprinter to move according to a dened path. The bioprinng process works through
the extrusion of a bioink or hydrogel biomaterial combined with human cells in a boom-
up, layer-by-layer fashion unl a 3D construct is built. Once the construct has been
bioprinted, it is crosslinked using the UV LED curing system or ionic soluons, depending
on your bioink’s crosslinking requirements.
2.1 Specicaons
2.1.1 Bioprinng
Bioprinng Technology: Pneumac-based microextrusion system
Printheads: 2 pneumac-based extrusion printheads.
1 UV LED curing system 365 nm (405 nm oponal)
Build Volume: 130 x 80 x 50 mm. Printbed has Insets for P100 petri dish and mul-
well plates
Posioning Precision: XY: 10 µm [0.0004 in]; Z: 2.5 µm [0.0001 in]
Layer Resoluon: 100 µm [0.0039 in]
Hydrogel’s viscosity range: 0.001 to 250 Pa.S [1 to 250,000 cP]
Max. Operang Pressure: 700 kPa
Set Pressure Range: 5 to 400 kPa
Sensivity: Within 0.2% F.S. (0.8 kPa)
Repeatability: Within ±1% F.S. (4 kPa)
Minimum Unit Seng: 1 kPa
Pressure Display Units: kPa, MPa, kgf/cm2, bar, psi, inHg and mmHg
Printhead’s Response Time: 5 ms or less (ON), 4 ms or less (OFF)
Nozzle Length: Auto-calibraon, 6.35 mm [0.25 in] to 38.1 mm [1.5 in]
Nozzle Diameter: User dependent, 50 to 1540 µm

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2.1.2 Soware
Soware Bundle: Slic3r, Repeer-Host, HeartWare
File Types: STL/OBJ/AMF
Supports: Windows (XP 32 bit/7+), Mac OS X (10.6 64 bit/10.7+) and Ubuntu Linux
(12.04+)
Connecvity: USB, SD-card
2.1.3 Physical specicaons
Frame: Chemically resistant, powder-coated high grade steel
D x W x H: 330 x 370 x 380 mm [19.1 x 16.5 x 14.7 in]
Shipping Box: 59 x 55 x 43 cm [23 x 21.5 x 17 in]
Shipping Weight: 20 kg [44.5 lbs]
2.1.4 Electrial
Power Supply Adapter:
Input: 100-240VAC, 50/60Hz, 2.0A
Output: 24VDC, 6.67A, 160W Max
Portable, Oil-free Air Compressor:
EU/UK/AU/CH: 230VAC, 50Hz
US/MEX/CA/JAP/Taiwan: 110VAC, 60Hz
INKREDIBLE 3D Bioprinter:
Input: 24 VDC, 6 A
2.1.5 IP-classicaon
IP10
CAUTION! If you want to use another air supply than the one provided by
CELLINK, please contact us to avoid damage to your INKREDIBLE 3D Bioprinter.

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3. Unpacking your INKREDIBLE 3D
Bioprinter

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Grab
here!
Grab
here!
www.CELLINK.com
When unpacking your INKREDIBLE 3D Bioprinter, be careful not to grab onto any
movable parts or lose components. We recommend you grab it by the side window
frames and pull it out from the box.
Once the INKREDIBLE 3D Bioprinter has been carefully taken out of the box, all protecve
lms, zip es and plasc parts prevenng the axes to move during transportaon can
be removed. Once all protecve materials have been removed, the INKREDIBLE can
be placed at its intended place of operaon (for example inside a biological hood or
laboratory bench).
WARNING! Always be two people present when unpacking your INKREDIBLE
3D bioprinter to avoid personal injury.
CAUTION! The INKREDIBLE should never be placed on wet, damp, moist or
humid surfaces.
CAUTION! Do not force or tear anything during the unpacking or setup process
as this may irreversibly damage your INKREDIBLE 3D Bioprinter.

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3.1 Contents of the box
[1x] INKREDIBLE 3D Bioprinter
[1x] User manual
[1x] Electrical supply box containing:
[1x] 24 V Power supply
[1x] Power cord
[1x] USB cable
[1x] Consumables box containing:
[2x] 3mL cartridge with CELLINK START
[4x] 3mL cartridge with piston and end caps
[2x] Blue conical nozzles (GA 22)
[2x] Red conical nozzles (GA 25)
[2x] White conical nozzles (GA 27)
[1x] Straight stainless steel nozzle (GA 22)
[1x] 24 well plate
[1x] Glass petri dish
[1x] UV-protecve goggles
[1x] Tube with adapter to air compressor
[1x] Air supply box containing:
[1x] 150 W, 300 kPa portable, oil-free air compressor
[1x] STARTINK-kit containing:
[1x] Cell syringe
[1x] Prelled syringe with 3 mL of CELLINK Bioink
[1x] Dispensing unit
[1x] Mixing unit
[1x] Filling cartridge
[2x] 5 ml of crosslinking soluon
[1x] Material safety data sheet
[1x] Applicaon note

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4. Product map

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INKREdibLE
2a
2b
5a 5b 6
9
81
7a 7c
7b
3
4
10a
10b
13 1412
11 a 7c 7b 11 b

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[1] On/O Buon
[2a] Printhead 1 (Ph 1)
[2b] Printhead 2 (Ph 2)
[3] UV LED
[4] Printbed
[5a] Digital pressure monitor for printhead 1
[5b] Digital pressure monitor for printhead 2
[6] SD-card reader
[7a] Printbed adjustment buery screw
[7b] Printbed adjustment buery screw
[7c] Printbed adjustment buery screw
[8] Control knob
[9] Main LCD display
[10a] Cartridge air connector for printhead 1
[10b] Cartridge air connector for printhead 2
[11a] Pressure regulator for printhead 1
[11b] Pressure regulator for printhead 2
[12] Power input
[13] Pressure connector
[14] USB input

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5. Operang your INKREDIBLE 3D
Bioprinter