Cellink INKREDIBLE User manual

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INKREdIBLE
User manual
CELLINK AB
Arvid Wallgrens Backe 20
Gothenburg, SE 41346
SWEDEN

Hello and welcome to the excing world of
3D Bioprinng!
We are excited to have you here. The technology
you have in front of you has the potenal of creang
real, live human ssues and it is only your imaginaon
that limits what you can do. This manual will serve
as your guide for seng up your INKREDIBLE 3D
Bioprinter, maintaining it, and also nofying you
about safety precauons to ensure a long-lasng
product lifeme. Make sure you read the enre
manual before using the product for the rst me.
Let`s begin this excing journey!

1. Safety informaon 4
1.1 General safety informaon 6
1.2 Protecve equipment 7
1.3 Liquids and dispensing media 7
1.4 Opening the product and service 7
2. Introducon to your INKREDIBLE 3D Bioprinter 8
2.1 Specicaons 10
3. Unpacking your INKREDIBLE 3D Bioprinter 12
3.1 Contents of the box 15
4. Product map 18
5. Operang your INKREDIBLE 3D Bioprinter 20
5.1 Seng up for rst me use 22
5.2 Starng a print 23
5.3 Advanced features 26
6. How to install and setup HeartWare 28
6.1 Installing Heartware (PC) 30
6.2 Installing Repeer-Host (Mac) 34
6.3 Seng up HeartWare 39
7. Slicing with HeartWare 42
7.1 What is Slicing? 44
7.2 HeartWare interface 45
7.3 Basic slicing workow 49
8. Maintenance and support 54
8.1 Maintaining your INKREDIBLE 3D Bioprinter 56
8.2 Support for your INKREDIBLE 3D Bioprinter 58
9. Warranty and guarantee 62
Contents

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1. Safety informaon

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WARNING! This symbol will indicate if there is risk of personal injury.
CAUTION! This symbol will indicate if there is risk of causing damage to your
INKREDIBLE 3D Bioprinter or other equipment.
Before you start using your INKREDIBLE 3D bioprinter, please take me to review the
enre manual. Maloperaon of the INKREDIBLE 3D bioprinter instrument can lead to
severe and irreversible injuries if the safety instrucons are not followed. Throughout the
manual we will use the following two symbols to indicate if there is risk for equipment
damage or personal injury:
In order to respond to the INKREDIBLE 3D Bioprinter and its relevant security
requirements, the instrument has to be operated by trained personnel, who are aware
of the risks and dangers that can be induced to themselves, as well as for the sta
being in close proximity with the INKREDIBLE 3D Bioprinter. The sta being involved
in the installaon, or the maintenance of the system or a part of the system, has to be
qualied through appropriate training. Use the instrument only for its intended purpose
as described in the documentaon. Do not modify the instrument, sub-components or
accessories.
1.1 General safety informaon
WARNING! Never reach into the instrument unl all components of the instrument
have come to a complete stop. Moving parts can cause serious injuries.
WARNING! Before cleaning, inspecng, adjusng, repairing or disassembling the
instrument or the dispensing units, stop the instrument, turn o the main switch
and disconnect if from its electrical power source.
WARNING! The INKREDIBLE 3D Bioprinter is ed with a 365nm (405nm
oponal) UV LED. Do not look directly at the light source. Always wear protecve
gear when operang the system, including UV protecve goggles.
WARNING! Improper mounted pressurized air spouts, liquid spouts or cartridges
can be dangerous. Verify that all connecons are properly connected and all
components are specied for use with the intended pressure. Verify that the
supplied air pressure does not exceed 400 kPa.
In addion to the manual, we are connously uploading instrucon videos on our
YouTube-channel, CELLINK3D. To access it go to:
hp://ny.cc/rbz7xy

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1.2 Protecve equipment
WARNING! Always wear protecve goggles and gloves while handling dangerous
materials that can be absorbed by skin or which are toxic, strike-aracve,
corrosive, causing allergies, carcinogenic, reproducon-endangering or mutagenic
to humans.
WARNING! Always read the material safety data sheets, packing labels,
manufacturer or dealer’s catalogue rst and use adequate safety equipment.
1.3 Liquids and dispensing media
WARNING! Before starng to work with hazardous dispensing materials,
the operator has to be informed about the characteriscs of the material and
its compability with the dispensing unit. The informaon can be found in the
material safety data sheets, packaging labels, manufacturer or dealer catalogues.
Always use adequate safety equipment.
1.4 Opening the product and service
WARNING! The INKREDIBLE 3D Bioprinter SHOULD NOT be taken apart. Doing
so endangers both the users and the equipment! The INKREDIBLE 3D Bioprinter
IS NOT user servicable! Opening of the instrument’s electrical panel will void the
warranty.
WARNING! Always wear protecve goggles and gloves, and secure the environment
around instrument from other personnel while operang the INKREDIBLE 3D
bioprinter. All safety equipment, including UV protecve safety goggles, must be
used when operang the UV curing system.

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2. Introducon to your INKREDIBLE
3D Bioprinter

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The INKREDIBLE 3D bioprinter is a pneumac-based extrusion bioprinter with dual
printheads and UV LED curing system for bioprinng of complex human ssue models
and organs for ssue engineering research. It is a cost-eecve unit for innovators
to enter the 3D bioprinng eld and start bioprinng living ssues at ease. It can be
used as a standalone unit thanks to its LCD controller or it can be monitored through
a computer with the use of the accompanied soware. Your three-dimensional CAD
models are translated into coordinates and instrucons for the INKREDIBLE to allow the
bioprinter to move according to a dened path. The bioprinng process works through
the extrusion of a bioink or hydrogel biomaterial combined with human cells in a boom-
up, layer-by-layer fashion unl a 3D construct is built. Once the construct has been
bioprinted, it is crosslinked using the UV LED curing system or ionic soluons, depending
on your bioink’s crosslinking requirements.
2.1 Specicaons
2.1.1 Bioprinng
Bioprinng Technology: Pneumac-based microextrusion system
Printheads: 2 pneumac-based extrusion printheads.
1 UV LED curing system 365 nm (405 nm oponal)
Build Volume: 130 x 80 x 50 mm. Printbed has Insets for P100 petri dish and mul-
well plates
Posioning Precision: XY: 10 µm [0.0004 in]; Z: 2.5 µm [0.0001 in]
Layer Resoluon: 100 µm [0.0039 in]
Hydrogel’s viscosity range: 0.001 to 250 Pa.S [1 to 250,000 cP]
Max. Operang Pressure: 700 kPa
Set Pressure Range: 5 to 400 kPa
Sensivity: Within 0.2% F.S. (0.8 kPa)
Repeatability: Within ±1% F.S. (4 kPa)
Minimum Unit Seng: 1 kPa
Pressure Display Units: kPa, MPa, kgf/cm2, bar, psi, inHg and mmHg
Printhead’s Response Time: 5 ms or less (ON), 4 ms or less (OFF)
Nozzle Length: Auto-calibraon, 6.35 mm [0.25 in] to 38.1 mm [1.5 in]
Nozzle Diameter: User dependent, 50 to 1540 µm