Cellink Bio x User manual

BIO X

User manual

CELLINK AB

Arvid Wallgrens Backe 20

Gothenburg, SE 41346

SWEDEN

Welcome to the world of bioprinng!

Not too long ago, the idea of creang living human ssue was no

more than a dream. Not anymore. The technology in front of you

is the next step on the path of regenerave medicine.

BIO X is designed with your research in mind. By simplifying

the 3D bioprinng process, you can focus on your research and

dream bigger than ever before.

Featuring unrivalled usability and versality, BIO X will help take

your research to the next level. With interchangeable intelligent

printheads, Clean Chamber technology, print chamber sterilizaon,

temperature-controlled print bed and a user-friendly interface,

BIO X is ready for the most complex 3D bioprinng challenges.

This manual will serve as your guide to geng started with BIO

X. Review the enre manual before using the product for the rst

me.

Welcome to the future of regenerave medicine!

Last update: 2019-03-12

Revision number 1.0

Contents

1. Safety informaon...................................................................................................................................4

1.1 General safety informaon...................................................................................................6

1.2 Unpacking, liing and carrying.............................................................................................7

1.3 Electrical informaon.............................................................................................................7

1.4 Protecve equipment ...........................................................................................................7

1.5 Hazardous materials...............................................................................................................8

1.6 Compliance..............................................................................................................................8

1.7 Electrostac discharge protecon......................................................................................9

2. Specicaons..........................................................................................................................................10

2.1 Product map...........................................................................................................................12

2.2 Technical specicaons........................................................................................................14

3. Geng started........................................................................................................................................16

3.1 Unpacking BIO X....................................................................................................................18

3.2 Contents ..................................................................................................................................19

3.3 Seng up BIO X.....................................................................................................................20

3.4 Starng BIO X for the rst me..........................................................................................21

4. Bioprinng with BIO X..........................................................................................................................22

4.1 Clean Chamber.......................................................................................................................24

4.2 Bioprinng from a 3D model...............................................................................................25

4.3 Bioprinng droplets...............................................................................................................38

4.4 Ulies......................................................................................................................................43

5. Maintenance............................................................................................................................................50

5.1 Changing the HEPA lter.....................................................................................................52

5.2 Manual cleaning or sterilizaon..........................................................................................52

6. Terms and condions.............................................................................................................................54

6.1 General terms and condions.............................................................................................56

6.2 End User Licence Agreement (EULA).................................................................................61

6.3 Warranty...................................................................................................................................68

7. Printhead manuals...................................................................................................................................70

7.1 CELLINK pneumac-based printhead................................................................................72

1. Safety informaon

Review the enre manual, as well as the manuals for any accessories, before you start using BIO X.

Mishandling can lead to equipment damage and serious injury. The following symbols are used to

indicate risk of equipment damage or personal injury:

CELLINK AB

Arvid Wallgrens Backe 20

Gothenburg, SE 41346

Sweden

1.1 General safety informaon

If BIO X acts in a way that is not described by this manual, turn it o and contact CELLINK.

Use BIO X for its intended purposes only. Do not modify its instruments, subcomponents or

accessories. Do not open BIO X, disassemble BIO X or aempt any service other than the instrucons

described in the manual.

This symbol indicates presence of UV light. Keep the BIO X door closed and wear

proper safety are during UV sterilizaon and UV curing. This symbol appears on the

touchscreen when a UV light source is acve.

Never place your ngers near the machine unl all parts have stopped moving. Moving

parts can cause injury.

This symbol indicates risk of injury or equipment damage.

Never clean or service BIO X while the machine is on. Always turn o the power and

disconnect any external sources of power and air pressure before cleaning or servicing

BIO X.

This symbol indicates a heated surface. BIO X has heated surfaces that may cause

personal injury or equipment damage if not treated with care. A physical symbol labels

these surfaces on the BIO X, and the symbol appears on the BIO X touchscreen when

the print chamber is heated.

Manufacturer

BIO X uses UV light for sterilizaon and curing. Never look directly at UV light. Never

expose skin to UV light. Eye or skin exposure can result in serious personal injury.

BIO X has heated surfaces that can reach temperatures up to 250 degrees Celsius.

Never touch heated surfaces when using the heang funcon. Always let printheads

and print bed cool aer using the heang funcon.

Always ensure that equipment is correctly mounted before use. Improperly mounted

print beds, printheads, cartridges, cables, liquid spouts and air spouts can be dangerous. If

any equipment appears damaged, turn o the machine, unplug all external connecons

and contact CELLINK before further use.

1.2 Unpacking, liing and carrying

BIO X is heavy. Always use a minimum of two people to unpack and move the machine.

Read how to safely unpack and setup BIO X in Chapter 3.

1.4 Protecve equipment

1.3 Electrical informaon

Always wear gloves and eye protecon during bioprinng, UV curing and sterilizaon.

Always wear gloves and eye protecon while handling hazardous materials, including

materials that are toxic, corrosive and carcinogenic.

Read the material safety data sheets, packing labels and the manufacturer or distributor’s

catalog before using the BIO X.

Always connect BIO X to a grounded socket and use the voltage described in the

specicaons. Improper electrical handling can cause equipment damage and personal

injury.

Never tamper with any safety interlocks. Tampering risks equipment damage and

personal injury.

1.5 Hazardous materials

1.6 Compliance

Consult material safety data sheets, packaging labels and the manufacturer or distributor

catalog before handling hazardous dispensing materials, such as ammable and

corrosive materials. Ensure any operators are informed of the material’s characteriscs

and compability with the dispensing unit. Always use appropriate safety equipment

and are. If you are unsure of a material’s compability, contact CELLINK.

Aer using ethanol to clean BIO X, wait at least 60 seconds before bioprinng to

ensure that all ammable vapors are removed.

By using BIO X, you agree to the terms stated here and in the warranty (Chapter 6.) Except for

the express warranty set forth in the warranty informaon and in our soware license terms as

applicable, all equipment and soware provided hereunder are provided “as is,” and CELLINK makes

no warranty as to its use or performance. Except for any warranty, condion, representaon or term

the extent to which cannot be excluded or limited by applicable law, CELLINK and its suppliers make

no warranty, condion, representaon, or term (express or implied, whether by statute, common

law, custom, usage or otherwise) as to any maer including, without limitaon, noninfringement

of third party rights, merchantability, integraon, or tness for a parcular purpose. You assume

responsibility for selecng the soluon to achieve your intended results, and for the installaon

of, use of, and results obtained from the equipment and soware. Without liming the foregoing

provisions, CELLINK makes no warranty that the equipment or soware will be error-free or free from

interrupons or other failures or that the soware or equipment will meet your requirements. Under

no circumstances and under no legal theory, whether in tort, contract, or otherwise, shall CELLINK

or its suppliers be liable to purchaser or to any other person for loss of prots, loss of goodwill, or

any indirect, special, incidental, or consequenal damages, or damages for gross negligence of any

character. Under no circumstances can CELLINK be responsible for consequences of misuse of

the instrument or for use of the instrument outside the use specied in this user manual, be they

material or personal.

1.7 Electrostac discharge (ESD) protecon protecon

1.7.1 Introducon

The BIO X print chamber is ESD-sensive. Use the following procedures to ensure that no damage

is caused to the BIO X or its accessories.

1.7.2 Procedures

Review the procedures to follow when using the BIO X print chamber and handling printheads.

Always discharge yourself before touching any ESD-sensive parts.

1.7.2.1 Discharging yourself

To discharge yourself, touch a grounded metal surface. Discharge yourself before touching printheads

or installing them in the BIO X. Examples of grounded metal surfaces include:

• Any metal surface in a laminar ow hood.

• Any metal sink.

• The frame of the BIO X when connected to a grounded power outlet, as well as any exposed

screws, including the screws on the le-side door hinges.

1.7.2.2 Using proper clothing

Avoid charging yourself to ensure ESD protecon. Wear clothes made out of coon when appropriate.

2. Specicaons

2.1 Product map

3a 3b

4a 5

2a 2b

1. Clean Chamber ™ unit

2a. UV sterilizaon LED 1

2b. UV sterilizaon LED 2

3a. Printhead mount 1

3b. Printhead mount 2

3c. Printhead mount 3

4a. Photo-curing module mount 1

4b. Photo-curing module mount 2

5. Print bed

6. 7-inch touchscreen

7. USB port

8. Protecve door

4 5 6

1. Power switch

2. Fuse

3. Power socket

4. USB port

5. Ethernet port

6. Pneumac port

2.2 Technical specicaons

Power input: 100-240 VAC, 50-60 Hz, 600 W

Fuse: 250VAC, T8A

Air pressure input: 0-700 kPa

Weight: 17 kg

Height: 477 mm

Width: 441 mm

Depth: 365 mm

Filter class: HEPA 14

Operate BIO in a room with a temperature between 20 and 23 degrees Celsius. In normal operang

condions, the chamber temperature will rise between 2 and 3 degrees, and the standard pneumac

printheads will rise between 4-5 degrees. For precise temperature control, use a temperature-

controlled printhead and ensure the room temperature is between 20 and 23 degrees.

3. Geng started

3.1 Unpacking BIO X

Follow these steps to unpack BIO X.

Do not aempt to unpack BIO X without reviewing the procedure below. Doing so

risks personal injury and equipment damage.

BIO X is heavy. Always use a minimum of two people to unpack and move the BIO X.

1. Clear a steady table and oor space of at least two by three meters.

2. Place the box on its side according to the arrows on the surface.

3. Carefully remove the tape on the top of the box.

4. Use the packaging handles to remove the contents of the box and place them on the oor.

5. Li the packaging carefully to turn the BIO X so it is standing on its correct side.

6. Remove the top piece of the packaging. Grab it at the boom edge to li BIO X out of the

boom piece of packaging. Place BIO X on the table.

7. Remove any remaining packaging material from BIO X.

8. Remove the remaining contents of the box and check that you have

received all equipment listed below.

This side up during transport

Bioink consumables box containing:

[2x] 3 mL CELLINK bioink (sterile)....................................................................................................................