Cellink BIO X User manual

BIO X Temperature-controlled Printhead
User Manual

Contents
1. Package contents
2. Technical specicaons
3. Safety informaon
3.1 BIO X system warnings
3.2 Temperature-controlled Printhead warnings
4. Geng started
4.1 Unpacking and installaon
4.2 Oponal: Aaching nozzle/needle insulator
4.3 Raising and lowering the printhead
4.4 Loading the bioink cartridge
4.5 Removing the printhead
4.6 Your rst bioprint
5. Temperature- controlled Printhead opmizaon
6. Relevant G-code commands
7. Frequently asked quesons
8. Maintenance
Appendix A: Consumables
Support informaon
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Package contents
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01 Package contents
Item
Temperature-controlled Printhead
Locking screw
Part number
000000020346
000000020106
Quanty
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Technical specicaons
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• Compable with 3-mL plasc cartridge.
• Dimensions (height x width x depth): 90 x 46 x 58 mm.
• Weight: 183 g.
• Maximum pressure: 700 kPa.
• Material composion:
• External surfaces: Aluminum.
• Internal surfaces: Aluminum.
• Recommended operang temperature: 20-23 degrees Celsius.
NOTE: We recommend operang BIO X in temperatures between 20 and 23 degrees. In normal operang
condions, the chamber temperature of the BIO X will rise 2-3 degrees above the ambient temperature.
• Running temperature (in recommended condions): 26-32 degrees.
• Heang capacity and rate:
• Total heang range: 65 degrees.
• Heang rate: Room temperature-65 degrees (10 minutes).
• Cooling capacity and rate:
• Minimum temperature: 17 degrees below chamber temperature.
• Cooling rate: Room temperature-Δ17 degrees (15 minutes).
• Build volume (X, Y, Z): 128 x 85 x (80–A) mm; A represents p length.
02 Technical specicaons

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Figure 1: Theorecal maximum build volume shown from the top and side view when using the Temperature-controlled Printhead.
Exact build volume depends on the cartridge’s posion in the printhead and the nozzle/needle used. Build volume may be lower when
combining the Temperature-controlled Printhead with a printhead that restricts printbox movement. Please consult the printhead
manual for the printheads being used for specic limitaons.
Top View Front View Isometric View
02 Technical specicaons

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02 Technical specicaons
Table 1: Theorecal build volume based on common p lengths. Actual volume may vary based on build plate thickness, size,
shape, level, cartridge posion and nozzle ghtness. X, Y and Z are measured from the front le corner of the printbed.
Tip Type Tip Length Nozzle/
Needle Length X Y Z Build Volume
Conical 3.2 cm 3.2 cm 12.7 cm 9.36 cm 4.8 cm 570 cm3
Blunt 2.4 cm 0.635 cm 12.7 cm 9.36 cm 5.6 cm 665 cm3
Blunt 3.0 cm 1.27 cm 12.7 cm 9.36 cm 5.00 cm 594 cm3
Blunt 4.3 cm 2.54 cm 12.7 cm 9.36 cm 3.70 cm 440 cm3
Micron-S 1.9 cm 1.9 cm 12.7 cm 9.36 cm 6.12 cm 727 cm3

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Safety informaon
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03 Safety informaon
3.1 BIO X system warnings
• Please consult the BIO X manual for BIO X-specic warnings and safety procedures.
3.2 Temperature-controlled Printhead warnings
• DO NOT clean the printhead by submerging it in liquid or using excessive spraying. Liquid inside the printhead can damage the circuitry and
motor.
• The LED on the printhead will turn yellow to indicate a warm printhead and red to indicate a hot printhead. Do not touch the printhead when
the LED is red. Touching a hot printhead risks serious injury.
• DO NOT manually move the printhead mount. Moving the printhead mount manually will damage the motor. If the mount needs to be moved,
place the printhead in the loading posion described in the Geng Started secon.
• The Temperature-controlled Printhead can be hot to the touch aer use. DO NOT touch the foils when the printhead is heang or cooling.
• DO NOT manually move the printhead mount. Moving the printhead mount manually will damage the motor. If the mount needs to be moved,
place the printhead in the loading posion described in the Geng Started secon.
• Use the proper method to load and unload the EMD Printhead onto the BIO X printhead mounts. Instrucons are outlined in the Geng
Started secon. While loaded on the BIO X, do not pull or push the printhead with excessive force, especially when the BIO X system is turned
on. Doing so will damage the motor and its guides.
• DO NOT use a jerking moon to remove the printhead. Using a jerking moon risks hing and damaging the HEPA lter.
• DO NOT place ngers on the printhead while it is in moon. Users risk serious, permanent injury if ngers are caught between the printhead
base and the motor arm.
• The cooling fan of the Temperature-controlled Printhead is exposed to allow for heat dissipaon. DO NOT put ngers or other objects into the
fan while the printhead is in operaon. Doing so risks injury and damage to the printhead.
cooling.

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Geng started
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04 Geng started
NOTE: The prinng parameters displayed on this manual might not be accurate for your specic protocol. For recommended
parameters please consult the documentaon for the bioink being used.
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Figure 2: Temperature-controlled Printhead components.
1. Cardge Adapter
2. Cardge
3. Printhead Body
4. Fan
5. Heat Sink
6. Locking Screw

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04 Geng started
Unpacking and installaon
• Open the package. Remove the Temperature-controlled printhead.
• Check for locking screw (Figure 2).
• Check the fan and heatsink for any debris.
• Oponal: Aach needle/nozzle insulator:
• Ensure that the nozzle/needle insulator has the right gauge for the diameter of the nozzle/needle being used.
• Align the insulator, screws and nozzle (Figure 3A).
• Insert the collar of the insulator into the Temperature-controlled Printhead.
• Tighten the screws to secure the insulator to the printhead (Figure 3B).
Figure 3: Aaching the nozzle/needle insulator to the Temperature-controlled Printhead.
AB

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04 Geng started
• To install the printhead, stabilize the boom of the printhead mount with one hand to avoid straining the motor. Align the printhead above the
desired printhead mount and push downward using your opposite hand (Figure 4).
Figure 4: Inserng the Temperature-controlled Printhead into the BIO X system.
AB C D

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04 Geng started
Raising and lowering the printhead
• On the user interface, navigate to the Ulies menu and then go to the Tools submenu.
• Lower the desired printhead mount into the acve or loading posion by pressing the down arrow (Figure 5).
• Press the up arrow to return the printhead to the nonacve posion.
Figure 5: Lowering the printhead into the acve posion.

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04 Geng started
Preheang or precooling the Temperature-controlled Printhead
• Set a temperature in the BIO X soware using the Ulies menu and Tools submenu. When cooling, the
operang temperature range of the Temperature-controlled Printhead has a maximum of 17 degrees below the
chamber temperature; when heang, the maximum temperature is 65 degrees (Figure 6).
• Wait for the the Temperature-controlled Printhead to reach the set temperature.
Figure 6: Preset temperature for the Temperature-controlled Printhead.
NOTE: We recommend precooling or preheang
your bioink in the cartridge outside the printhead
in an incubator, water bath or refrigerator before
use. If using a water bath, ensure the cartridge is
dry before pung it in the printhead.

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04 Geng started
Loading the bioink cartridge
• Fill a cartridge with bioink or use a prelled cartridge.
• Remove the p cap (Figure 7A). Aach a nozzle (Figure 7B). Remove the end cap (Figure 7C).
Figure 7: Preparing a prelled bioink cartridge.
AB C

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04 Geng started
Figure 8: Aaching the air adapter to the cartridge.
• Align the cartridge adapter to the loaded cartridge (Figure 8A). Twist unl secure (Figure 8B).
AB