Cellink BIO X EMD User manual

BIO X EMD Printhead
User Manual

Contents
1.Package contents 1
2.Technical specicaons 3
3.Safety Informaon 7
3.1 BIO X system warnings 8
3.2 EMD Printhead warnings 8
4.Geng started 11
4.1 Unpacking 12
4.2 Installing, raising, lowering and uninstalling the
printhead 14
4.3 Removal and inseron of microvalve and nozzle 16
4.4 Reassembling the printhead 19
4.5 Loading a cartridge 20
4.6 Tesng Extrusion 25
4.7 Your rst bioprint 28
4.8 Removing printhead 35
4.9 Prinng opmizaon 37
5.Relevant G-code commands 39
6.Frequently asked quesons 43
7.Maintenance 45
7.1 Cleaning 46
7.2 Long-term maintenance 49
Appendix A: Consumables- needles and nozzles 51
Available nozzles and part numbers 52
Compable consumables 53
Support informaon 55

1
Package contents
01

2
01 Package contents
Item Part number Quanty
EMD Printhead 000000020566 1
EMD accessory tool 000000020557 1
Luer lock adaptor D16110020558 1
Cartridge airline adapter with transparent tube 3cc 12 cm 000000010040 1
EMD microvalve 000000020551 1
Threaded nozzle (needleless) D16110020555 1
Threaded nozzle (with needle) 000000020560 1
Needle cap 000000020556 1
Flushing tube with Luer adaptor, 20 cm D16110020912 + 000000010110 1
3cc cartridge (inside EMD) CSC010300102 1

3
Technical specicaons
02

4
02 Technical specicaons
• Compable with 3-mL cartridge.
• Maximum bioink volume in cartridge: 3 mL.
• Dimensions (height x width x depth): 118 x 30.5 x 37.5 mm.
• Weight: 132 g.
• Maximum pressure: 700 kPa.
• Material composion:
• External surfaces: Powder-coated aluminum.
• Internal surfaces: Aluminum.
• Internal uid chamber (valve): Stainless steel.
• Recommended operang temperature: 20-23 degrees Celsius.
NOTE: We recommend operang BIO X in temperatures between 20 and 23 degrees. In normal operang condions, the chamber
temperature of the BIO X will heat up 2-3 degrees above room temperature.
• Running temperature (in recommended condions): 26-32 degrees.
• Heang capacity and rate:
• Total heang range: 30-60 degrees.
• Heang rate: Room temperature -40 degrees (10 minutes).
• Heang rate: 40-65 degrees (12 minutes).
• Cooling capacity: N/A.
• Build volume (X, Y, Z): 128 x 85 x (65 – A) mm (A = p length).

5
02 Technical specicaons
Figure 1: Theorecal maximum build volume shown from the top and side view using the EMD Printhead (assuming a
nozzle length of 0 mm). Exact build volume depends on the cartridge’s posion in the printhead and the nozzle/needle
used. Build volume may be lower when combining the EMD Printhead with a printhead that restricts printbox move-
ment. Please consult manuals for the specic printheads used to determine potenal restricons.

6
02 Technical specicaons
Nozzle Type Tip
Length
Nozzle/ Needle
Length
X Y ZBuild Volume
Standard 4 mm 4 mm 12.8 cm 8.5 cm 6.5 cm 702.2 cm3
Table 1: Theorecal build volume based on nozzle length. Actual volume may vary based on build plate’s thickness, size,
shape and level, as well as on cartridge posion and ghtness of the nozzle. X, Y and Z are measured from the front le
corner of the printbed.

7
Safety informaon
03

8
03 Safety informaon
3.1 BIO X system warnings
• Please consult the BIO X manual for BIO X-specic and general warnings and safety procedures.
3.2 EMD Printhead warnings
• Do not clean the printhead by submerging it in liquid or using excessive spraying. Liquid inside the printhead can damage the circuitry and
motor. To clean the EMD valve, you must remove it from the printhead and follow instrucons in the Maintenance secon.
• The LED on the printhead will turn yellow to indicate a warm printhead and red to indicate a hot printhead. Do not touch the printhead when
the LED is red. Touching a hot printhead risks serious injury.
• Do not manually move the printhead mount. Moving the printhead mount manually will damage the motor. If the mount needs to be moved,
place the printhead in the loading posion described in the Geng Started secon.
• Use the proper method to load and unload the EMD Printhead onto the BIO X printhead mounts. Instrucons are outlined in the Geng
Started secon. While loaded on the BIO X, do not pull or push the printhead with excessive force, especially when the BIO X system is
turned on. Doing so will damage the motor and its guides.
• Do not use a jerking moon to remove the printhead. Using a jerking moon risks hing and damaging the HEPA lter.
• Handle the threaded nozzle and needle with care or the needle may bend and aect dispensing.
• Handle the microvalve end with care. When inserng and removing the valve, do not touch the outlined area (Figure 2).
• Avoid using tools or excessive force to ghten the nozzle into the printhead.

9
03 Safety informaon
Figure 2: Do not touch the outlined area of the EMD microvalve.
.

10
03 Safety informaon
• Do NOT remove the valve, nozzle or cartridge from the EMD printhead while it is aached to the printhead mount. The cartridge will be
pressurized upon aachment and untwisng these components will result in bioink leakage.
• Parcles or pigment contained in the bioinks can limit the lifeme of the microvalve.
• Contaminants or dirt parcles can clog the microvalve and other components of the EMD Printhead.

11
Geng started
04

12
04 Geng started
NOTE: The prinng parameters displayed on this manual might not be accurate for your specic protocol. For recommended
parameters please consult the documentaon for the bioink being used.
4.1 Unpacking
1. Open the package. Remove the pre-assembled EMD Printhead and other components (Figure 3).
2. The pre-assembled EMD printhead contains components A, B, C, D and F. Component E is provided in a separate protecve container, and
component G is a tool for the removal of component F.

13
04 Geng started
Figure 3: Package contents include:
A. EMD Printhead body.
B. Microvalve.
C. Nozzle cover for storage.
D. Needleless threaded nozzle.
E. Threaded nozzle.
F. Luer lock adapter.
G. EMD accessory tool.
A
B
C D E F
G

14
04 Geng started
4.2 Installing, raising, lowering and uninstalling the printhead
1. To install the printhead in the BIO X, stabilize the boom of the printhead mount with one hand to avoid straining the motor. Align the
printhead above the desired printhead mount and push downward using your opposite hand (Figure 4).
Note: for more informaon on how to assemble the printhead, see Secon 4.3.
Figure 4: Inserng the EMD Pneumac Printhead into the BIO X system.

15
04 Geng started
2. On the user interface, navigate to the Ulies menu and then go to the Tools submenu.
3. Lower the desired printhead mount into the acve or loading posion by pressing the down arrow (Figure 5).
Figure 5: Lowering the printhead into
the acve posion.

16
04 Geng started
4. Press the up arrow to return the printhead to the nonacve posion.
5. To remove the printhead, use one hand to stabilize the printhead mount and the other hand to push the printhead slightly upward.
4.3 Removal and inseron of microvalve and nozzle
1. Ensure the printhead has been removed from the BIO X (Secon 4.2).
2. Remove the nozzle cover from the EMD Printhead to reveal the nozzle (Figure 6A).
3. Unscrew and remove the threaded nozzle (Figure 6B, D, E).
4. Grip the edge of the microvalve (Figure 6F) being careful not to contact the glass p (Figure 2). Pull straight down to remove the microvalve
from the EMD Printhead (Figure 6C, G). If necessary, twist to remove it.