Cellink BIO X EMD User manual

BIO X EMD Printhead
User Manual

Contents
1.Package contents 1
2.Technical specicaons 3
3.Safety Informaon 7
3.1 BIO X system warnings 8
3.2 EMD Printhead warnings 8
4.Geng started 11
4.1 Unpacking 12
4.2 Installing, raising, lowering and uninstalling the
printhead 14
4.3 Removal and inseron of microvalve and nozzle 16
4.4 Reassembling the printhead 19
4.5 Loading a cartridge 20
4.6 Tesng Extrusion 25
4.7 Your rst bioprint 28
4.8 Removing printhead 35
4.9 Prinng opmizaon 37
5.Relevant G-code commands 39
6.Frequently asked quesons 43
7.Maintenance 45
7.1 Cleaning 46
7.2 Long-term maintenance 49
Appendix A: Consumables- needles and nozzles 51
Available nozzles and part numbers 52
Compable consumables 53
Support informaon 55

1
Package contents
01

2
01 Package contents
Item Part number Quanty
EMD Printhead 000000020566 1
EMD accessory tool 000000020557 1
Luer lock adaptor D16110020558 1
Cartridge airline adapter with transparent tube 3cc 12 cm 000000010040 1
EMD microvalve 000000020551 1
Threaded nozzle (needleless) D16110020555 1
Threaded nozzle (with needle) 000000020560 1
Needle cap 000000020556 1
Flushing tube with Luer adaptor, 20 cm D16110020912 + 000000010110 1
3cc cartridge (inside EMD) CSC010300102 1

3
Technical specicaons
02

4
02 Technical specicaons
• Compable with 3-mL cartridge.
• Maximum bioink volume in cartridge: 3 mL.
• Dimensions (height x width x depth): 118 x 30.5 x 37.5 mm.
• Weight: 132 g.
• Maximum pressure: 700 kPa.
• Material composion:
• External surfaces: Powder-coated aluminum.
• Internal surfaces: Aluminum.
• Internal uid chamber (valve): Stainless steel.
• Recommended operang temperature: 20-23 degrees Celsius.
NOTE: We recommend operang BIO X in temperatures between 20 and 23 degrees. In normal operang condions, the chamber
temperature of the BIO X will heat up 2-3 degrees above room temperature.
• Running temperature (in recommended condions): 26-32 degrees.
• Heang capacity and rate:
• Total heang range: 30-60 degrees.
• Heang rate: Room temperature -40 degrees (10 minutes).
• Heang rate: 40-65 degrees (12 minutes).
• Cooling capacity: N/A.
• Build volume (X, Y, Z): 128 x 85 x (65 – A) mm (A = p length).

5
02 Technical specicaons
Figure 1: Theorecal maximum build volume shown from the top and side view using the EMD Printhead (assuming a
nozzle length of 0 mm). Exact build volume depends on the cartridge’s posion in the printhead and the nozzle/needle
used. Build volume may be lower when combining the EMD Printhead with a printhead that restricts printbox move-
ment. Please consult manuals for the specic printheads used to determine potenal restricons.

6
02 Technical specicaons
Nozzle Type Tip
Length
Nozzle/ Needle
Length
X Y ZBuild Volume
Standard 4 mm 4 mm 12.8 cm 8.5 cm 6.5 cm 702.2 cm3
Table 1: Theorecal build volume based on nozzle length. Actual volume may vary based on build plate’s thickness, size,
shape and level, as well as on cartridge posion and ghtness of the nozzle. X, Y and Z are measured from the front le
corner of the printbed.