Cellink BIO X 10-mL User manual

BIO X 10-mL Pneumac Printhead
User Manual

Contents
1.Package contents 1
2.Technical specicaons 3
3.Safety informaon 9
3.1 BIO X system warnings 10
3.2 Pneumac Printhead warnings 10
4.Geng started 11
4.1 Unpacking and installaon 12
4.2 Raising and lowering the printhead 13
4.3 Loading the bioink cartridge 14
4.4 Your rst bioprint 16
4.5 Removing the printhead 23
4.6 Prinng opmizaon 25
5.Relevant G-code commands 27
6.Frequently asked quesons 31
7.Maintenance 35
Support informaon 37

1
Package contents
01

2
01 Package contents
Item Part number Quanty
BIO X 10 mL Pneumac Printhead 000000020356 1
Air adapter connector 10 mL 000000610041 1

3
Technical specicaons
02

4
02 Technical specicaons
• Compable with 10-mL plasc cartridge.
• Dimensions (height x width x depth):
• With screw: 63.5 x 30.5 x 51 mm.
• Without screw: 63.5 x 30.5 x 46.2 mm.
• Weight: 102 g.
• Maximum recommended pressure: 700 kPa.
• Material composion:
• External surfaces: Powder-coated aluminum.
• Internal surfaces: Aluminum.
• Recommended operang temperature: 20-23 degrees Celsius.
NOTE: We recommend operang the BIO X in temperatures between 20 and 23 degrees. In normal operang condions,
the chamber temperature of the BIO X will rise 2-3 degrees above room temperature, and the 10-mL Pneumac Printhead
temperature will rise above the chamber temperature.

5
02 Technical specicaons
• Running temperature (in recommended condions): 26-32 degrees.
• Heang capacity and rate:
• Total heang range: 30-65 degrees.
• Heang rate: Room temperature-40 degrees (10 minutes).
• Heang rate: 40-65 degrees (12 minutes).
• Cooling capacity: N/A.
• Build volume (X, Y, Z): 128 x 85 x (83.4–A) mm; A represents p length.

6
02 Technical specicaons
Figure 1:





7
02 Technical specicaons
Table 1:


Tip Type Tip Length Nozzle/Needle Length X Y Z Build Volume
Conical      
Blunt      
Blunt      
Blunt      
Micron-S      

8

9
Safety informaon
03

10
03 Safety informaon
3.1 BIO X system warnings
• Please consult the BIO X manual for BIO X-specic and general warnings and safety procedures.
3.2 10-mL Pneumac Printhead warnings
• Do not clean the printhead by submerging it in liquid or using excessive spraying. Liquid inside the printhead can
damage the circuitry and motor.
• The LED on the printhead will turn yellow to indicate a warm printhead and red to indicate a hot printhead. Do not
touch the printhead when the LED is red. Touching a hot printhead risks serious injury.
• Do not manually move the printhead mount. Moving the printhead mount manually will damage the motor. If the
mount needs to be moved, place the printhead in the loading posion described in the Geng Started secon.
• Use the proper method to load and unload the 10-mL Pneumac Printhead onto the BIO X printhead mounts.
Instrucons are outlined in the Geng Started secon.
• Do not use a jerking moon to remove the printhead. Using a jerking moon risks hing and damaging the HEPA
lter.
• Tighten the locking screw before prinng to ensure that the cartridge remains in place during the prinng process.
Improper ghtening can cause the cartridge to move and material to dispense improperly.
• Do not touch a moving printhead. Fingers can be crushed between the printhead base and motor arm and can result
in serious injury.

11
Geng started
04

12
04 Geng started
4.1 Unpacking and installaon
NOTE: The prinng parameters displayed on this manual might not be accurate for your specic protocol. For
recommended parameters please consult the documentaon for the bioink being used.
1. Open the package. Remove the 10-mL Pneumac Printhead.
2. Aach the 10-mL Pneumac Printhead to the BIO X printbox. Align the printhead above the desired printhead mount (Figure 2A) and push
downward using one hand. Secure the boom of the prinhead mount with your opposite hand to avoid straining the motor (Figure 2B,C,D).
ABC D
Figure 2:



13
04 Geng started
4.2 Raising and lowering the printhead
1. On the user interface, navigate to the Ulies menu and then go to the Tools submenu.
2. Lower the desired printhead mount into the acve or loading posion by pressing the down arrow (Figure 3).
3. Press the up arrow to return the printhead to the inacve posion.
Figure 3:


14
04 Geng started
4.3 Loading the bioink cartridge
1. Make sure that the printhead is installed and in the acve or loading posion (Secon 4.1 and 4.2)
(Figure 2).
2. Fill a cartridge with bioink or obtain a prelled cartridge. Remove the boom cap if using a prelled
cartridge (Figure 4A). Aach a nozzle (Figure 4B) and remove the end cap (Figure 4C).
3. Align the cartridge adapter to the loaded cartridge (Figure 4D). Twist unl secure (Figure 4E).
ABC
Figure 4:
D E

15
04 Geng started
4. Load the cartridge into the printhead from the top (Figure 5B). Tighten the locking screw. To make this step easier, we
recommend placing the printhead in the acve posion (lowered) as explained in the Raising and lowering the printhead secon
(page 13) (Figure 5A).
5. Connect the air tubing to the air inlet on the printbox above the respecve printhead (Figure 5C).
6. Raise the printhead to the inacve posion (Secon 4.2).
Figure 5:
ABC

16
04 Geng started
4.4 Your rst bioprint
1. Prepare the printhead and load the bioink as detailed in Secon 4.3.
2. Select Bioprint from the Start menu (Figure 6).
Figure 6:.