cetoni Nemesys M User manual

Nemesys M
Hardware Manual
ORIGINAL INSTRUCTIONS 1.00 –APRIL 2021

2CETONI Nemesys M Hardware Manual
CETONI GmbH
Wiesenring 6
07554 Korbussen
Germany
T+49 (0) 36602 338-0
F+49 (0) 36602 338-11
E[email protected]
www.cetoni.de

CETONI Nemesys M Hardware Manual 3
The information and data contained in this document are subject to change without notice. CETONI
GmbH is constantly striving to develop all its products. This means that there may be changes in form,
equipment and technology. Claims can therefore not be made on the basis of information, illustration or
descriptions in these instructions. The description for the product specification in this manual does not
constitute an integral part of the contract.
If you control the products with a software from CETONI GmbH, you agree to the applicable license
agreement, which can be read in the corresponding software manual. This and all other current product
manuals can be found at https://www.cetoni.de/en/downloads/manuals.
CETONI GmbH grants its customers the right to reproduce this manual for the purpose of providing
technical information to potential users of CETONI products. Extracts of these documents may only be
reproduced or transmitted with a precise reference to the authorship of CETONI GmbH, irrespective of
the manner in which they are reproduced or transmitted, either electronically or mechanically. Any
duplication or use of excerpts for other purposes requires the written permission of CETONI GmbH.
We are always open to comments, corrections and requests. Please send them to [email protected].
The general terms and conditions of CETONI GmbH shall apply. Alternative agreements must be in
written form.
Copyright © CETONI GmbH –Automation and Microsystems. All rights reserved.

4CETONI Nemesys M Hardware Manual

CETONI Nemesys M Hardware Manual 5
1Overviews & Directories
1.1 Table of Contents
1Overviews & Directories 5
1.1 Table of Contents 5
1.2 Revision History 7
2Introduction 8
2.1 Preface 8
2.2 Symbols and Keywords Used 8
2.3 Norms and directives 9
2.4 Application Purpose 9
2.4.1 General Description of the Device 9
2.4.2 Intended Use 9
2.4.3 Reasonably Foreseeable Faulty Application 9
2.4.4 Safety measures 10
2.4.5 Measures for Safe Operation 11
2.5 Warranty and Liability 12
3Scope of Delivery 13
4Technical Data 14
4.1 Environment 14
4.2 Mechanical Data 14
4.3 Electrical Data 14
4.4 Interfaces 14
4.5 Dimensional Drawing 15
4.6 Dosing Performance 16
4.7 Materials (Syringes) 17
5Operating the Hardware 19

6CETONI Nemesys M Hardware Manual
5.1 Initial Startup 19
5.2 Mounting a Syringe 20
5.3 Fluidic Connection 24
5.3.1 First-Time-Tube-Installation 25
5.3.2 Tube Disassembly 26
5.3.3 Tube Reassembly 27
5.4 Upright Operation 27
5.5 Accessory Port 28
5.6 RS-232 Connection 29
5.6.1 Pin Assignment of Module Interfaces 29
5.6.2 OEM RS232 Cable Set 29
5.7 Overload shut-off 31
5.8 Syringe Maintenance 32
5.8.1 Piston Removal/Installation 32
5.8.2 Piston (Dis)Assembly 34
5.8.3 Front Cap Removal/Installation 36
5.8.4 Mounting the seal wash set 37
5.8.5 Maintenance and Care 38
6Transport and Storage 39
7Maintenance and Care 40
8Disposal 41

CETONI Nemesys M Hardware Manual 7
1.2 Revision History
REV
DATE
CHANGE
VALID FOR
1.00
08.04.2021
Creation
Type NEM-B124-01 A
IMPORTANT. In its current revision, this manual applies only to the product types
listed in the last line. Should you require a manual from a previous revision, please do not
hesitate to contact us. Please let us know your device type and email address and we will
send you the appropriate manual as a pdf file.
The type of your product can be found on the label behind "Type:", according to the marked number in
the following example:

8CETONI Nemesys M Hardware Manual
2Introduction
2.1 Preface
Thank you for purchasing a product from CETONI. With this user manual we would like to support you
as well as possible when handling the device. If you have any questions or suggestions, please do not
hesitate to contact us.
2.2 Symbols and Keywords Used
The following symbols are used throughout this manual to help you navigate through this document:
HINT. Indicates application tips and useful hints to facilitate operation.
IMPORTANT. Indicates important information and other particularly useful
information that does not describe dangerous or harmful situations.
ATTENTION. Indicates a potentially harmful situation. If it is not avoided, the product
or something in its environment may be damaged.
CAUTION. Indicates a potentially dangerous situation. If it is not avoided, slight or
minor injuries and property damage may result.

CETONI Nemesys M Hardware Manual 9
2.3 Norms and directives
CETONI GmbH declares under its sole responsibility, that the CETONI Nemesys M
complies with the health and safety requirements of the relevant European directives.
2.4 Application Purpose
2.4.1 General Description of the Device
The CETONI Nemesys M is a syringe pump. It allows emptying and filling syringes by the relative linear
movement of a syringe- and a piston holder.
2.4.2 Intended Use
The CETONI Nemesys M is used for highly precise and low-pulsation dosing of fluids in the range of
nanoliters to milliliters per second. Pressures of over 100 bar can be achieved.
Application usually takes place in laboratory-like rooms.
2.4.3 Reasonably Foreseeable Faulty Application
A use for applications distinct from the intended purpose can lead to dangerous situations and is to be
omitted.
CAUTION. The CETONI Nemesys M must not be used as a medical device or for
medical purposes.
CAUTION. It is not allowed to use the CETONI Nemesys M in an explosive atmosphere
or with potentially explosive substances.

10 CETONI Nemesys M Hardware Manual
2.4.4 Safety measures
The safety of the user and a failure-free operation of the CETONI Nemesys M are assured only if original
parts are used. Only original accessories may be used. Warranty claims will not be accepted for damage
due to the use of alien accessories or expendables.
The CETONI Nemesys M has been developed and constructed in such a way as to largely rule out
hazards due to its intended use. Nevertheless, you must observe the following security measures in
order to exclude any remaining hazards:
CETONI GmbH points out the responsibilities of the operator for the operation of the devices.
The laws and regulations of the place of installation must be observed while operating the
devices! To ensure a safe work routine, operators and users must assume responsibility for
adhering to regulations.
Before operating the device, the user must ensure the functional safety and proper condition of
the device, its accessories and the cables. The device must not be operated if there is any visible
damage.
The user must be familiar with the operation of the devices and the software.
Cables must be laid in a way that avoids any risk of stumbling.
Any moving parts must not be touched whilst the devices are in operation. There is a risk of
crushing!
Relieve the pressure in the system before loosening connections.
Check the leak tightness of all fluidic connections after connection and at regular intervals.
Only use connection material that is specified for the expected pressures.
The device is designed and approved to work in fluidic systems, which fall within the scope of
Article 4 Paragraph 3 of the Pressure Equipment Directive 2014/68/EU. This means that the
system may not exceed a maximum volume of 1 liter. With the use of fluids from Group 1
according to Article 13 of the Pressure Equipment Directive 2014/68/EU, the maximum
allowable system pressure is 200 bar. For fluids from Group 2 it is 1000 bar. If different,
product-specific values for the maximum pressure are given in the section "Technical Data",
these values must be complied with. Regarding the maximum operating temperature, the
specification from the section "Technical Data" must be observed.
CETONI GmbH is not liable for consequences that may arise if the user expands the system by
peripheral devices, such that one of the values or both values are exceeded.

CETONI Nemesys M Hardware Manual 11
It is the user's responsibility to become familiar with the mentioned Pressure Equipment
Directive and to comply with the prevailing requirements.
Wear protective glasses and, if necessary, additional personal protective equipment when
working with corrosive, hot or otherwise dangerous substances during assembly work on the
device.
Transportation, storage or operation of the devices below 0°C with water in the fluid passages
may cause damage to the modules.
2.4.5 Measures for Safe Operation
2.4.5.1 ELECTROMAGNETIC EMISSIONS
The CETONI Nemesys M is intended for use in any type of facility, connected directly to the public
power supply network that supplies buildings used for domestic purposes.
2.4.5.2 ELECTROSTATIC DISCHARGE
Floors should be made of wood, concrete, or ceramic tiles. If the flooring is made of a synthetic material;
the relative humidity must be at least 30%.
2.4.5.3 ELECTRIC DISTURBANCES
The quality of the supply voltage should be to the standard of a typical business or hospital
environment.
2.4.5.4 MAGNETIC DISTURBANCES
Do not place power connector cables, even of other appliances, in close proximity of the devices and
their cables. Mobile communication devices may not be used in closer proximity of the devices or their
cables than the recommended safety distance!
2.4.5.5 SAFETY DEVICES ON THE SYSTEM
The system can be switched off at any time in an emergency using the mains switch on the Base
Module (toggle switch on the side of the housing); this will cause no damage to the unit.
2.4.5.6 CONDITION OF THE DEVICES
Irrespective of the faultless manufacture of the devices, damage can occur whilst the unit is in
operation. With this in mind, always carry out a visual check of the components mentioned before use.
Pay particular attention to crushed cables, damaged tubing, and deformed plugs. If you should notice
any damage, please do not use the devices and inform CETONI GmbH without delay. CETONI will put

12 CETONI Nemesys M Hardware Manual
your devices back to an operational condition at the earliest. Do not attempt to repair the devices
yourself.
2.5 Warranty and Liability
The devices left our company in perfect condition. Only the manufacturer is permitted to open the
devices. All warranty and liability entitlements, particularly damage entitlements due to personal
injuries, are void if the devices are opened by an unauthorised person.
The duration of the warranty is 1 year of technical equipment (expect wear parts) from the day of
delivery. It is not extended or renewed due to work carried out under warranty.
CETONI GmbH considers itself responsible for the devices with regard to safety, reliability and function
only if assembly, new settings, changes, extensions and repairs are carried out by CETONI GmbH or an
authorised centre, and if the devices have been used in accordance with the instruction manual.
The product conforms to the basic safety regulation standards. Industrial property rights are reserved on
the circuits, methods, names, software programs, and units.

CETONI Nemesys M Hardware Manual 13
3Scope of Delivery
The following items should be included:
CETONI NEMESYS M
Star nut for syringe fixation
Other accessories such as syringes, tubing material, pressure sensors etc. have to be purchased
separately.

14 CETONI Nemesys M Hardware Manual
4Technical Data
4.1 Environment
TEMPERATURE (OPERATION)
-20 –50 °C
TEMPERATURE (STORAGE )
-40 –75 °C
AIR HUMIDITY
10% to 90%, non-condensing
4.2 Mechanical Data
DIMENSIONS (L x W x H)
310 x 56 x 108 mm
WEIGHT
2,3 kg
4.3 Electrical Data
SUPPLY VOLTAGE (POWE R SUPPLY UNIT)
24 V DC
POWER CONSUMPTION
24 W
4.4 Interfaces
CAN
1 Mbit/s
RS-232
Section 5.6
ACCESSORY PORT
Section 5.5

CETONI Nemesys M Hardware Manual 15
4.5 Dimensional Drawing

16 CETONI Nemesys M Hardware Manual
4.6 Dosing Performance
The following table gives an overview of the minimum and maximum dosing speeds and the resulting
flow rates for the CETONI stainless steel syringes. Below the speeds and flow rates referred to as
pulsation-free, the dosing precision slowly decreases.
Furthermore, the table shows the maximum pressure achievable with the respective syringe.
Supplementary explanations can be found in section 5.7.
You need the nominal and maximum stroke to configure the syringes in the software. Please read the
corresponding sections in the software manual.
Speed
Minimum travel
[nm]
Minimal
[pm/s]
Minimal pulsation free
[nm/s]
Maximum
[mm/s]
0,765
168,141
6
5,605
Syringe
Nominal stroke/
Maximum stroke
[mm]
Flow Rate
Pressure
[bar / psi]
Minimal
[pl/s]
Minimal pulsation free
[nl/s]
Maximum
[ml/s]
3 ml
59,616 / 61
38,508
8,461
0,302
199 / 2882
5 ml
58,797 / 61
65,073
14,298
0,510
118 / 1706
10 ml
58,878 / 61
129,968
28,558
1,019
59 / 854
25 ml
59,106 / 61
323,668
71,119
2,538
24 / 343
50 ml
59,145 / 61
646,908
142,142
5,072
12 / 172

CETONI Nemesys M Hardware Manual 17
4.7 Materials (Syringes)
The wetted parts of the syringes are made of stainless steel with the EN material number 1.4404 (316L).
As customer-specific variation the material can also be other stainless steels or even special alloys. For
evaluation of compatibility with the used reagents please refer to the particular material you are using.
O-rings are used as seals in the syringes. Either an O-ring in combination with a backup ring or a sliding
ring pretensioned with an O-ring can be used as a piston seal.
Backup rings prevent the O-ring from being damaged under high pressure. They are not in direct
contact with the medium, but come into contact with leakage. O-rings have significantly less leakage,
but wear out faster and generate more abrasion. Sliding rings wear more slowly, but do not seal as well.
The O-rings mounted as standard are made of FKM (fluoroelastomer), the sliding rings and backup rings
are made of PTFE with 25 % coal.
For the 3 ml and the 5 ml syringe, the O-ring hardness should not be less than 80 shore A, for the three
larger syringes 70 shore A is sufficient. The dimensions of the O-rings can be found in the following
table.
Ød1 = inner diameter
Ød2 = cord diameter
Example: O-ring 12x2 →Ød1=12; Ød2=2
SYRINGE
CAP
PISTON
(BACKUP RING)
PISTON
(SLIDING RING)
SEAL WASH SET
(PISTON)
SEAL WASH SET
(CYLINDER)
3 ml
10x1,5
5,5x1,5
5,5x1
7x1,5
17x1,5
5 ml
12x1,5
7x2
7x1,5
7x1,5
17x1,5
10 ml
16x1,5
11x2
11x1,5
7x1,5
17x1,5
25 ml
24x1,5
18x2,5
18x2
10x1,5
34x1,5
50 ml
34x1,5
28x2,5
28 x 2
10x1,5
34x1,5

18 CETONI Nemesys M Hardware Manual
Although the syringes are made of corrosion-resistant material, surface corrosion may be caused by
media residues during storage. For that reason, disassemble and clean the syringes prior to periods of
non-use.
ATTENTION. Before using the syringes, please check the compatibility of the materials
used with your medium.
ATTENTION. Transportation, storage or operation of the syringes below 0°C with
water in the fluid passages may cause damage.
IMPORTANT. When used for the first time, sliding rings require a few syringe strokes
until they achieve their full sealing effect. This can also be done manually and with an
empty syringe. Please also take this into account after a seal change.

CETONI Nemesys M Hardware Manual 19
5Operating the Hardware
5.1 Initial Startup
The initial start-up, i.e. connecting, configuring and separating a system, is described in the CETONI
System Manual. However, there are the following deviations when configuring the CETONI Nemesys M
and when separating:
(1) The CETONI Nemesys M is equipped with a rotary switch. This allows the module to be switched off
so that it can remain connected to the system while other modules are being configured. The
module that you want to configure 1must be switched on - the rotary switch is then in vertical
alignment and the status light lights up blue. All other modules 2must be switched off during
configuration - the rotary switch is turned 45° counterclockwise to OFF and the status light is off.
IMPORTANT. If the status light is red, the internal fuse of the device is defective. In
this case, please contact CETONI GmbH to clarify the further procedure.
Rotary Switch Turned 45°
Rotary Switch Vertical
2
Switched Off
1
Switched On
Is Being Configured
Status Light Off
Status Light On

20 CETONI Nemesys M Hardware Manual
(2) Separating the module from the system is done in a slightly different way than described in the
system manual, as the CETONI Nemesys M has a separating mechanism. To separate, press the
button 1on the front of the module firmly. This releases the plug connection to the previous
module. Then pull the other end of the module off the system by pulling the centering pin 2. You
can now remove the individual module and reconnect the bus terminating plug (terminator) to the
last module of the remaining system.
5.2 Mounting a Syringe
Before mounting a syringe on the CETONI Nemesys M, it must be configured and selected in the
software. The procedure is described in the software manual. For this you need the Volume (Scale
Volume), the nominal stroke (Scale Length) and the maximum stroke (Piston Stroke). The values for Scale
Volume and Scale Length are engraved on the CETONI stainless steel syringes. The Piston Stroke is 60
mm unless otherwise indicated. The values can also be found in the table in section 4.6. Further
information on the syringes can be found in the syringe manual.
1
2