CODEX DNA BioXp 3250 User manual

1
BioXp™ 3250 system — User guide
Part number 43029 |Revision 1.1 |Eective 11DEC2020

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Contents
LEGAL NOTICES 3
SAFETY INFORMATION 4
MANUAL SYMBOLS 4
PRECAUTIONS 4
MARKING SYMBOLS ON THE EQUIPMENT 4
SAFETY WARNINGS 5
EQUIPMENT SPECIFICATIONS 7
SYSTEM COMPONENTS 7
TECHNICAL SPECIFICATIONS 7
SITE PREPARATION AND INSTALLATION 8
SYSTEM SITE PREPARATION 8
GETTING TO KNOW THE BIOXP™ SYSTEM 8
HARDWARE OVERVIEW 8
INSTRUMENT PORTS AND POWER SWITCH 9
BIOXP™ APPLICATIONS 9
PLACING AN ORDER 9
SETTING UP THE BIOXP™ SYSTEM 10
LEAVE THE BIOXP™ SYSTEM POWERED ON 10
ETHERNET SETUP 10
START THE BIOXP™ SYSTEM 10
CONFIGURE THE BIOXP™ SYSTEM 11
TEST THE NETWORK CONNECTION 11
CHANGE THE CLOCK OPTIONAL 12
RUNNING THE BIOXP™ SYSTEM 12
RECEIVING A BIOXP™ APPLICATION PROJECT 12
PREPARING THE BIOXP™ SYSTEM FOR A RUN 13
LOADING THE BIOXP™ DECK 13
INITIATING A BIOXP™ RUN 14
CANCELLING A JOB DURING A RUN 14
BIOXP™ RUN COMPLETION 15
TROUBLESHOOTING 16
BIOXP™ DECK INSPECTION ERROR 16
GENERAL TROUBLESHOOTING 17
CLEANING AND MAINTENANCE 17
CLEANING INSTRUCTIONS 17
CALIBRATION 18
PREVENTATIVE MAINTENANCE 18
REPAIR 18
EQUIPMENT DISPOSAL 18
WEEE COMPLIANCE 18
REGULATORY STATEMENTS 18
FCC COMPLIANCE STATEMENT 18
KOREAN EMC COMPLIANCE STATEMENT 19
Legal notices
Limited use label license Except as otherwise agreed in writing by our authorized representative, this product is for INTERNAL
RESEARCH USE ONLY AND NOT FOR HUMAN, ANIMAL, THERAPEUTIC OR DIAGNOSTIC USE. For
additional information about your rights under this research license, visit codexdna.com.
Limited warranty a) Codex DNA, Inc.’s entire liability and your exclusive remedy if the Product fails to conform to the
following warranty (a “Nonconforming Product”) shall be, at Codex DNA, Inc.’s sole option, either repair or
replacement of such Nonconforming Product, or, if neither is practicable, a refund of the fees paid for the
Product. The warranty for the repaired or replaced Product is limited to the scope and remaining duration
of the original warranty for the Nonconforming product or, if longer, for 30 days after the date of shipment
to you of the repaired or replaced Product. The warranty is contingent upon proper use of the Product in
accordance with the user guide and does not apply to any Product that is subjected to unusual physical
or electrical stress, misuse, neglect, improper testing or storage, modification or unauthorized repair or
upgrade. b) Codex DNA, Inc. warrants that the product, as delivered, will be free from defects in material
and workmanship for a period of one year from the date it is delivered to you (“the Warranty Period”).
c) This warranty gives you specific legal rights and you may have other legal rights which vary from
state to state. This warranty is non-transferable. d) OTHER THAN AS EXPRESSLY SET FORTH ABOVE,
Codex DNA, Inc. MAKES NO WARRANTIES, EXPRESS, STATUTORY, IMPLIED, OR OTHERWISE. NO
DISTRIBUTOR, AGENT OR EMPLOYEE IS AUTHORIZED TO MAKE ANY MODIFICATIONS, EXTENSIONS
OR ADDITIONS TO THIS WARRANTY. e) You shall indemnify, defend and hold harmless Codex DNA, Inc.
from any costs, expenses, damages, or other losses arising out of i) any warranty of greater scope or
duration than that set forth in this Codex DNA, Inc. Limited Warranty; and ii) failure to disclaim implied
warranties and limit remedies and liabilities, by and on behalf of Codex DNA, Inc.
Trademark information Gibson Assembly® is a registered trademark and BioXp™ and Oligo Vault™ are trademarks of
Codex DNA, Inc.
Patents Gibson Assembly® US patent numbers 7,776,532, 8,435,736, and 8,968,999.
Regulatory statement For research use only.
Statement of
proprietary information
Copyright in this work is vested in Codex DNA, Inc. and the document is issued in confidence for the
purpose only for which it is supplied. It must not be reproduced in whole or in part except under an
agreement or with the consent in writing of Codex DNA, Inc. and then only on the condition that this
notice is included in any such reproduction. No information as to the contents or subject matter of this
document or any part thereof arising directly or indirectly there from shall be given orally or in writing or
communicated in any manner whatsoever to any third party being an individual firm or company or any
employee thereof without the prior consent in writing of Codex DNA, Inc.
Terms and conditions IMPORTANT — This agreement (the “Agreement”) is a legal agreement between you and
Codex DNA, Inc. By installing or otherwise using the BioXp™ 3250 system (referred in this agreement as
the “Product”), you agree to be bound by the terms of this Agreement. If you do not wish to be bound by
the terms of this Agreement, do not use the Product and promptly return the unused, unopened Product
to the place of purchase for a full refund. The user is prohibited from reverse engineering the software or
output files generated from the BioXp™ 3250 system.
Limitation of liability CODEX DNA, INC.’S LIABILITY ARISING OUT OF OR RELATING TO A PRODUCT SHALL NOT EXCEED
THE AGGREGATE AMOUNTS YOU PAID TO CODEX DNA, INC. FOR THE PRODUCT. IN NO EVENT
WILL CODEX DNA, INC. BE LIABLE FOR LOST USE, PROFITS, REVENUE, COST OF PROCUREMENT
OF SUBSTITUTE GOODS, OR ANY OTHER SPECIAL, INDIRECT, RELIANCE, INCIDENTAL, OR
CONSEQUENTIAL DAMAGES, HOWEVER CAUSED AND UNDER ANY THEORY OF LIABILITY. THE
FOREGOING LIMITATIONS SHALL APPLY REGARDLESS OF WHETHER CODEX DNA, INC. HAS BEEN
ADVISED OF THE POSSIBILITY OF SUCH DAMAGES AND NOT WITHSTANDING THE FAILURE OF
ESSENTIAL PURPOSE OF ANY LIMITED REMEDY.
Disclaimer The material in this manual is for informational purposes only and is subject to change without prior
notice at any time. Codex DNA, Inc. and/or its aliates assume no responsibility for any errors that may
appear in this document.
Indications for use The BioXp™ 3250 system is a device which is intended for the processing of DNA materials.
The system is not approved for use other than that stated above.
Technical services For technical assistance, contact [e-mail] or call 858.228.4115.

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Safety information
Manual symbols
This manual describes the use and maintenance of the Codex DNA BioXp™ 3250 system. Read this manual completely before
putting the instrument into service. The following icons are used in this manual:
Electrical safety warning: Failure to follow these instructions can cause harm to operators or subjects.
Important note: Failure to follow these instructions can lead to damage of the system or unexpected results.
Precautions
The BioXp™ system conforms to the following standards:
• IEC 61010-1:2010 third edition, EN 61010-1:2010, UL, CSA
• EMC: IEC 61326-1:2012; EN 61326-1:2013
• KN 61000-6-4: 2015 and KN 6100-6-2: 2015, AUS/NZ CISPR 11
The radiation exposure hazard is non-critical.
Covers and exterior components which the operator may come in contact with during routine maintenance or
calibration, shall operate at a voltage no greater than 25 V AC or 60 V DC.
Marking symbols on the equipment
Warning: Indicates a warning concerning operations that may lead to personal injury or
potential damage to the instrument if not performed correctly.
DO NOT MOVE
BY HAND
CAUTION
Caution, hot surface, do NOT touch.
Conformité European: This mark is a declaration by the manufacturer that the respective
component complies with the relevant directives and standards as issued by the European
Union.
Korean EMC compliance: This symbol indicates that the product conforms to relevant South Korean
EMC standards.
사용자안내문
사􏘓용􏘓자􏘓안􏘓내􏘓문
이􏘓기기는􏘓업무용􏘓환경에서􏘓사용할􏘓목적으로􏘓적합성평가를􏘓받은􏘓기기로서가정용􏘓환경에서􏘓사용하는
경우􏘓전파간섭의􏘓우려가􏘓있습니다.
사용자􏘓안내문은 “업무용􏘓방송통신기자재”에만􏘓적용한다.
Alternating current.
Waste Electrical and Electronic Equipment (WEEE) directive: Do not dispose symbol. For more
information, see Equipment disposal and WEEE compliance.
Consult instructions for use.
Warning: Flammable material.
Safety warnings
Installation
• The BioXp™ system is heavy. Do not attempt to lift or move the instrument without the assistance and
supervision of colleagues. Be certain to use appropriate moving equipment and proper lifting techniques
(including but not limited to making certain that you have a secure grip on the instrument before lifting,
keeping your spine in a neutral position while lifting with your legs, and coordinating lifting and moving
movements with all appropriate personnel). Improper lifting can cause permanent back injury.
• Use this product only as specified. If the instrument is used in a manner not specified by Codex DNA,
the protection provided by the instrument may be impaired, resulting in personal injury or damage to the
instrument.
Ground circuit protection
• Check the mains electricity supply plug, voltage, and frequency to confirm that the supply corresponds to
the values shown on the instrument label.
• Ensure that the supply of power to the instrument is via a socket fitted with a protection device, such as a
circuit breaker of the correct rating, to provide automatic power cut-o in the event of an insulation fault.
• Confirm that the mains wiring is grounded. Codex DNA declines all responsibility for any damages due to
instrument non-grounding.
Main power switch
Do not position the instrument such that it is dicult to operate the main power switch.

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Hazardous chemical warning
• Chemicals used with the instrument may be hazardous.
• Always wear protective gear including safety goggles, gloves, and a lab coat when handling chemicals.
• Exercise caution when handling flammable liquids.
• Take precautions, including but not limited to installing proper ventilation systems, to ensure that the BioXp™
system laboratory is safe and that operators of the instrument will not be exposed to hazardous levels of
harmful chemicals.
• Follow all national, state, and local health and safety regulations and laws.
• Use proper waste disposal in accordance with all relevant regulations.
• Refer to applicable material safety data sheets (MSDSs).
Flammable liquids: Instrument warning
• Exercise caution when using flammable liquids in the BioXp™ system to minimize the risk of fire.
• Do not use any flammable liquids in the instrument except for the liquids required in the included procedure.
• Provide adequate ventilation for the instrument to prevent the accumulation of flammable vapors.
• All sources of ignition, such as open flame or electrical spark, are not permitted near the instrument or in
areas where flammable vapors may travel.
• Ensure that the instrument, all neighboring equipment, and users of the instrument are appropriately
grounded to minimize the generation of static electricity.
• Do not use the ethanol reservoir if it is cracked.
Flammable liquids: Solution handling warning
• Keep flammable liquids in covered containers when not in use.
• Provide means to promptly and safely dispose of any flammable liquid leaks or spills.
• Do not transfer liquids using air pressure.
• Read applicable MSDSs.
• Store flammable liquids in cool, well-ventilated areas away from corrosives, oxidizers, and ignition sources.
• Label containers and cabinets as “flammable materials” where applicable.
• Use only approved safety vessels for flammable liquid storage.
• Ground and bond flammable liquid containers to prevent static charge buildup.
• Never pour flammable liquids down a drain or sink.
• Dispose of empty flammable containers in an approved manner.
Electrical safety warning
• Do not attach the power cord to an extension cord or to a multiple portable socket. Doing so may
compromise shielding and/or grounding.
• This equipment should not be used adjacent to or stacked with other equipment. If it becomes necessary
to use the BioXp™ system under these conditions, the unit should be observed to verify normal operation in
this condition.
• Use only grounded mains outlets to supply instrument.
• Do not remove any panels. Panels should be removed only by trained service personnel.
• The AC power cord is the primary disconnection device for the instrument. Ensure that the point of
connection is easily accessible and free of any obstructions.
Important warning: Moving parts
• Do not override the door lock. When the door is locked, the moving parts of the instrument are operational.
Never attempt to physically restrict movement of instrument components.
• Interfering with moving parts may cause personal injury. Keep hands clear of moving parts while operating
the instrument.
• Do not move or shift the instrument during operation.
• If the instrument shows evidence of corrosion or wear, do not attempt to manipulate or tamper with
components of the instrument.
Important warning: Disconnecting the instrument
• Disconnect power to the system before cleaning or performing maintenance.
• DO NOT remove any panels; they should be removed only by qualified service personnel.
• Do not disassemble unit.
Equipment specifications
System components
• Use of parts or materials other than those supplied with the instrument can degrade system performance
and may cause safety hazards. Use only Codex DNA approved or supplied components.
• Use only Codex DNA supplied power cord. Use of other power cords may compromise electrical protection
and could create a hazard.
Components of the BioXp™ system
• BioXp™ 3250 system
• Power cord
• RJ45 ethernet cable
• Reagent and recovery plate thermal covers
• Ethanol reservoir
Technical specifications
Product name BioXp™ 3250 system
Power input voltage 100–240 V
Power input current 8.3 A max
Operating temperature range 16 °C to 40 °C
Storage temperature range −18 °C to 60 °C
Operating and storage humidity range 10% to 90% (non-condensing relative humidity)
Operating altitude Up to 2,000 m
Water ingress Non-immersion, protection for damp wipe only
Weight 63.4 kg [139.8 US lbs]
Dimensions (W × H × D) 69 × 77 × 53 cm [27 × 30 × 21 in]

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Site preparation and installation
System site preparation
For details on site preparation, safety precautions, uncrating, setup, and powering up, see the BioXp™ 3250 system remote
installation and sign-o technical guide.
Getting to know the BioXp™ system
Hardware overview
Figure 1. Front view of the BioXp™ 3250 system Figure 2. The BioXp™ 3250 system deck
. . . .
BioXp™ DNA assembly plate
P/N:BX1230-XX Lot:123 MFGdate: 09/01/2020
4
1
2 3
5 6
9
11
10
12
8
7
13
1. Thermocycler lid 8. Strip holder
2. Thermocycler 9. Tip tray 1
3. Purification station 10. Tip tray 2
4. Thermal cover 11. Tip tray 3
5. Chiller 1 12. Tip tray 4
6. Chiller 2 13. Tip waste
7. Ethanol reservoir
Figure 3. Deck map
Instrument ports and power switch
Location Label no. Port/inlet Used to
Figure 4. Power on/o switch
and communications ports
1USB
Do not use.
Note: The USB port is for instrument
diagnostics and is for authorized
service personnel use only
2Ethernet Connect to the Codex DNA server for
system communication
3Fuse Do not use
4Power switch
Switch power to the instrument on
or o. Note: Leave the instrument on
continuously.
5Power inlet Power the instrument
BioXp™ applications
The BioXp™ 3250 system builds gene fragments, clones, and variant DNA libraries.
BioXp™ gene fragments
The BioXp™ system builds 32 high-quality individual dsDNA fragments, 300 bp to 1,800 kb in length, in less than 16 hours.
BioXp™ cloning projects
Using integrated Gibson Assembly® technology, the BioXp™ system builds and clones dsDNA fragments, up to 7,000 bp,
into custom vectors, providing transformation-ready products at the end of an automated run.
BioXp™ variant libraries
The BioXp™ system automates the construction of scanning and combinatorial libraries. By combining state-of-the-art
robotics, liquid handling, thermocycling, and on-deck purification, the BioXp™ system significantly reduces the hands-on
time required for synthetic dsDNA library construction and cloning.
Placing an order
To order custom BioXp™ application projects and reagents, visit the Codex DNA ordering portal at codexdna.com. After creating
an account, you will be able to upload sequences and place an order.

10 11
Setting up the BioXp™ system
Leave the BioXp™ system powered on
We recommend leaving the instrument on continuously. Only turn the instrument o when necessary, such as when moving the
instrument to another location. If the instrument must be turned o, toggle the power switch to the O position.
Ethernet setup
The BioXp™ system requires an internet connection. Prior to connecting the instrument to the internet, be sure to test the
connection. Connect the provided RJ45 ethernet cable to the ethernet port — located on the side of the unit — to an available
ethernet wall jack.
In order for the BioXp™ system to communicate with the Codex DNA server, you must open the 80/443 port through
your network. In addition, the BioXp™ system will be making an outbound SSL connection (HTTPS/SSL) to logmein.com,
codexdna.com and drive.google.com. Unblock or whitelist these sites.
Start the BioXp™ system
1. Ensure that all packaging materials have been removed, including the
shipping screw located under the pipette head.
2. Turn the instrument on using the power switch located on the side of the
instrument.
3. Close the instrument door. The BioXp™ system will initiate a self-check
process to verify the correct functionality of motion control and other
internal systems of the instrument. If the instrument is unable to perform a
successful self-check it will alert the user to the problem and will prevent
the instrument from operating until the problem has been addressed. The
self-check process has been carefully developed to ensure that the BioXp™
system is operating within optimal calibration ranges and that all systems are
functioning correctly before initiating a run.
Configure the BioXp™ system
1. Press Configuration
Insert new job materials and
close door to start the next job.
Or change the configuration.
BioXp™ system ready
Configuration
2. Press Network
Configuration
Date and time Service
Network Language
Done
Test the network connection
Press Test connection
If the system displays Network connection working normally,
the BioXp™ system is ready for use. If you are ready to load
the instrument and begin a run, refer to the appropriate deck
loading instructions for your system run.
Network
Cancel
IP address
10.32.36.110
Subnet mask
10.32.36.110
Default gateway
10.32.36.110
Preferred DNS server
MAC address
10.32.36.110
MAC address
 Use DHCP
Network connection
working normally
Test connection OK
11:03:1D:12:8AF7
If the system displays No network detected, contact
Codex DNA customer service at [e-mail] for
assistance in resolving the connection.
Network
Cancel
IP address
10.32.36.110
Subnet mask
10.32.36.110
Default gateway
10.32.36.110
Preferred DNS server
MAC address
10.32.36.110
MAC address
 Use DHCP
No network detected
Test connection OK
11:03:1D:12:8AF7
×

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Change the clock (optional)
Press Set date and time
Use the up and down arrows to set the time and date
manually. Then press OK.
Notes:
• Time is displayed in hours : minutes : seconds and the date
is displayed in month/day/year.
• Check DST to automatically update clock to daylight saving
time. Cancel OK
Time zone: (UTC -08:00) Pacific Time (US & Canada)
Time
2 : :14 36 PM
Date
04/ /09 2020
DST
Running the BioXp™ system
Once you’ve ordered and received your BioXp™ application project, running the BioXp™ system is as easy as loading the deck
and pressing Start.
Receiving a BioXp™ application project
From building gene fragments and clones to library construction, BioXp™ applications come with everything you need to
complete projects. The project components are made up of modules.
Gene
fragments Clones
Clones +
amplification
Variant
libraries
Module A (+4 °C) Oligo Vault™ plate 
Module A (+4 °C) Recovery plate 
Module A (+4 °C) DNA purification strip 
Module A (+4 °C) Custom vector strip —  —
Module B (−20 °C) DNA assembly reagent plate 
Module C (−20 °C) Gibson Assembly® cloning strip —  —
Module D (−20 °C) RapidAMP™ strip — — —
Preparing the BioXp™ system for a run
1. Load tips by aligning the tip tray notch with the upper left corner of each tip tray retainer.
a. Load 3 × 50 μL tips
b. Load 1 × 200 μL tips
2. Add a minimum of 12 mL freshly prepared 70% ethanol to the reusable ethanol reservoir.
a. Load ethanol reservoir in the right-most reservoir retainer position of the instrument deck.
Note: Do not discard the ethanol reservoir after the run; keep for future use.
Loading the BioXp™ deck
Refer to BioXp™ gene fragments and variant libraries — Loading map and checklist or the BioXp™ cloning projects — Loading
map and checklist document for project-specific loading instructions.
Reagent strips
BioXp™ gene fragments and variant libraries BioXp™ cloning + amplification projects
. . . .
BioXp™ DNA assembly plate
P/N:BX1230-XX Lot:123 MFGdate: 09/01/2020
BioXp™system
OligoVault™
OLIGO VAULT™ PLATE
RECOVERY PLATE
50 µL TIPS50 µL TIPS
50 µL TIPS 200 µL TIPS
DNA ASSEMBLY REAGENT PLATE
THERMAL COVER
ETHANOL RESERVOIR
REAGENT STRIPS
. . . .
BioXp™ DNA assembly plate
P/N:BX1230-XX Lot:123 MFGdate: 09/01/2020
BioXp™3200
Oligovault
OLIGO VAULT™ PLATE
RECOVERY PLATE
50 µL TIPS50 µL TIPS
50 µL TIPS 200 µL TIPS
DNA ASSEMBLY REAGENT PLATE
THERMAL COVER
ETHANOL RESERVOIR
REAGENT STRIPS
Refer to BioXp™ cloning projects — Loading map and checklist document for preparing custom vector strip.
Gene fragments
and variant libraries Cloning
Cloning and
RapidAMP™ amplification
. . . .
1
Pinholes
2 3 4
. . . .
1
Pinholes
2 3 4
. . . .
1
Pinholes
2 3 4
Position 1 — black DNA
purification strip
Position 2 — blue
RapidAMP™ strip
Position 3 — purple
Gibson Assembly®
cloning strip
Position 4 — red custom
BioXp™ vector strip

14 15
Initiating a BioXp™ run
1. Once the deck is loaded, confirm that all components are
securely seated.
2. Close the door.
3. Press Start. The run will initiate.
Cancel
START
2-FRAGMENT CLONE 16S + AMPLIFY V1.1 EM
2-FRAGMENT CLONE 16S + AMPLIFY V1.1 EM
Tracking progress during a run
Time to completion
During a run, the instrument will display the projected time
remaining for the run (in hours : minutes : seconds) and the
estimated time of completion.
2-fragment clone + amplify V1.1 M
Time remaining
00:00:29
Time of completion
3:07:59
Slide to cancel 
 Step 1 Step 2 Step 3 Step 4
Cancelling a job during a run
IMPORTANT! You will not be able to reuse reagents if you cancel a job after the run has initiated and the reagent plate seal has
been broken. Do not cancel a run unless absolutely necessary.
1. If you need to cancel a job during a run, slide your finger
to the right across the bar on the bottom of the display
screen during a run.
Slide to cancel 
2. After sliding to cancel, the system will display a
confirmation to cancel screen. Press YES — Cancel job to
end the run or No — Keep running to continue the run.
Are you certain you want to cancel this
job? You will not be able to restart it.
Attention
Yes –Cancel job No –Keep running
If you press YES — Cancel job, the instrument will display a
Canceling job screen until the job cancel is complete.
Attention
Cancelling job
Storing covers
Opening thermal cycler
3. When prompted, remove all materials and used or
partially used consumables from the instrument deck.
Attention
Open door and remove materials
from a previous job (if any remain).
Then close door to continue.
BioXp™ run completion
1. When the BioXp™ run is complete, open the door.
2. All DNA products are now located in the recovery plate. The plate map provided with the project identifies specific locations
of each fragment and final product. Seal and store the recovery plate at 2 °C to 8 °C for up to one week or at −20 °C for up to
one year.
3. Empty and dry the ethanol reservoir.
4. Empty the tip waste and discard remaining consumables.
BioXp™ gene fragments and variant libraries BioXp™ cloning projects
. . . .
BioXp™3200
Oligovault
BioXp™ DNA assembly plate
P/N:BX1230-XX Lot:123 MFGdate: 09/01/2020
OLIGO VAULT™ PLATERECOVERY PLATE
BioXp™3200
Oligovault
. . . .
BioXp™ DNA assembly plate
P/N:BX1230-XX Lot:123 MFGdate: 09/01/2020
OLIGO VAULT™ PLATERECOVERY PLATE

16 17
Troubleshooting
BioXp™ deck inspection error
After you load the deck and close the instrument door for
a system run, the instrument performs a deck inspection,
checking the location and orientation of the recovery plate,
ethanol reservoir, reagent strip handles, reagent strips, tips,
and system robotics.
Inspecting system
Inspecting recovery plate
Inspecting reservoir
Inspecting strip handles
If any anomalies are detected, the instrument displays a deck
inspection failure message, similar to the screen shown on the
right. If the system displays a deck inspection failure message,
see the following table for recommendations.
Deck inspection found problems:
Plate found in purification station.
Recovery plate not found or is rotated.
Reservoir missing or misplaced.
Strip handle not closed.
Attention
Ignore and continue Stop and fix
Issue Possible solution and recommendations
Deck inspection detected anomalies with one (or more) of
the following:
• Plate found in purification station
• Recovery plate not found or is rotated
• Ethanol reservoir is missing or misplaced
• Strip handle not closed
Press Stop and fix
• Open the door and correct the deck inspection issue.
• After fixing the error, close the door to initiate a second
deck inspection.
• If the deck inspection error message persists after you
have corrected any issues, call Codex DNA technical
services.
Error messages
If you observe an error screen during operation, follow the
screen instructions. If directed or if the problem persists, make
a note of the screen and call Codex DNA technical services.
Additional information is included in the following table.
 Read job issue. Open the door to retry.
If this persists, call 855.474.4362.
Attention
Unlock door
General troubleshooting
Codex DNA technical services: 858.228.4115
Issue Possible solution and recommendations
Touchscreen does not light up • Confirm that the power switch is in the ON position.
• Confirm that the instrument is plugged into a working
electrical outlet.
• Verify that the plug is securely connected to the side of
the instrument.
• Contact Codex DNA technical services.
Power failure occurs during the run
Contact Codex DNA technical services.
Diculty operating the touchscreen
Cleaning and maintenance
Cleaning instructions
• Clean the exterior of the BioXp™ system with standard cleaners and a damp cloth.
• Clean the internal surfaces periodically using a mild detergent or 70% ethanol. Spray the cleaner onto a cloth and then use the
damp cloth to wipe instrument surfaces. Do not spray any cleaners directly into the instrument.
Do not use abrasive or caustic cleaners on internal or external instrument surfaces.
Spills
Take every precaution to ensure that no liquids are spilled in the instrument. Wipe up accidental spills in and around the
instrument immediately. If ethanol spills, dispose of ethanol-saturated towels according to your institutional guidelines.
In the event of hazardous material spillage
• In the event of spillage of a hazardous material, the BioXp™ system user is responsible for decontamination of the hazardous
material on or inside the instrument.
• Before using a cleaning or decontamination method other than a recommended cleaner, contact Codex DNA to verify that the
proposed decontamination method will not damage the equipment.

18 19
Calibration
Calibration of the motion control system is performed by the manufacturer. Yearly verification of the system calibration is
recommended for optimal performance. The instrument will perform a self check each day. A log of the self check is stored on
the system and may be used by Codex DNA service technicians should any issue arise. Keeping the BioXp™ system connected
to the internet ensures optimal use and analysis of the calibration log.
Preventative maintenance
The BioXp™ system requires periodic cleaning and lubrication of the motion system. This procedure is ideally performed annually
and is included in the optional service contract. Contact customer service for warranty and service contract inquiries.
Repair
Each BioXp™ system has been manufactured and calibrated to specifications. Do not attempt to disassemble any part of the
system. Any such action will void the product warranty. In the event you should experience a product failure, call Codex DNA
technical services at 858.228.4115.
Equipment disposal
This product has parts that contain a small amount of mercury and lead in some components. Disposal of these materials may
be regulated in your community due to environmental considerations. Follow local regulations for disposal of equipment or
equipment accessories at the end of their life.
WEEE compliance
The BioXp™ system complies with the European Union’s Waste and Electrical and Electronic directive. For disposal
or recycling information, contact your local authorities or the electronic industry alliance: www.eiae.org.
Regulatory statements
FCC compliance statement
This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of the FCC
rules. These limits are designed to provide reasonable protection against harmful interference in a commercial or medical
installation. This equipment generates, uses and can radiate radio frequency energy and, if not installed and used in accordance
with the instructions, may cause harmful interference to radio or television reception. However, there is no guarantee that
interference will not occur in a particular installation. If this equipment does cause interference to radio and television reception,
which can be determined by turning the equipment o and on, the user is encouraged to try to correct the interference by one
or more of the following measures:
• Reorient or relocate the receiving antenna
• Increase the separation between the equipment and the receiver
• Connect the equipment into an outlet on a circuit dierent from that to which the receiver is connected
• Consult the dealer or an experienced radio/TV technician for help
The connection of a non-shielded equipment interface cable to this equipment will invalidate the FCC certification of this device
and may cause interference levels which exceed the limits established by the FCC for this equipment. It is the responsibility of
the user to obtain and use a shielded equipment interface cable with this device. If this equipment has more than one interface
connector, do not leave cables connected to unused interfaces. Changes or modifications not expressly approved by the
manufacturer could void the user’s authority to operate the equipment.
Portable and mobile RF communication devices such as cellular phones can adversely aect medical equipment.
Korean EMC compliance statement
Type of equipment Additional information
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Class A equipment:
Business, broadcasting and communication equipment
This device has been evaluated for conformity for use in a
business environment.
If used in a home environment, there is a danger of
interference.
Guidance and manufacturer’s declaration: Emissions
All equipment and systems
Emission tests Compliance Electromagnetic environment guidance
RF emissions CISPR 11 Group 1
The BioXp™ system uses RF energy only for its internal function. Therefore,
its RF emissions are very low and are not likely to cause any interference in
nearby electronic equipment.
RF emission CISPR 11 Class A
The BioXp™ system is suitable for use in all commercial establishments.
Harmonic emission IEC
61000-3-2 Class A
Voltage fluctuations IEC
61000-3-3 Complies

Guidance and manufacturer’s declaration: Immunity
All equipment and systems
The BioXp™ system is a class A device and is intended for use in the electromagnetic environment specified below.
The customer or user of the BioXp™ system should ensure that it is used in such an environment.
Immunity test IEC 60601 test level Compliance level Electromagnetic environment guidance
Electrostatic discharge
(ESD) IEC 61000-4-2
± 6 kV contact
± 8 kV air
± 6 kV contact
± 8 kV air
Floors should be wood, concrete, or ceramic tile.
If floors are covered with synthetic material, the
relative humidity should be at least 30%.
Electrical fast transient/
burst
IEC 61000-4-4
± 2 kV for power supply
lines
± 1 kV for input/output
lines
± 2 kV for power supply
lines
± 1 kV for input/output
lines
Mains power quality should be that of a typical
commercial or laboratory environment.
Surge
IEC 61000-4-5
± 1 kV dierential mode
± 2 kV common mode
± 1 kV dierential mode
± 2 kV common mode
Mains power quality should be that of a typical
commercial or laboratory environment.
Voltage dips, short
interruptions, and
voltage variations on
power supply input lines
IEC 61000-4-11
< 5% UT (> 95% dip in
UT) for 0.5 cycle
40% UT (60% dip in UT)
for 5 cycles
70% UT (30% dip in UT)
for 25 cycles
< 5% UT (> 95% dip in
UT) for 5 sec.
< 5% UT (> 95% dip in
UT) for 0.5 cycle
40% UT (60% dip in UT)
for 5 cycles
70% UT (30% dip in UT)
for 25 cycles
< 5% UT (> 95% dip in
UT) for 5 sec.
Mains power quality should be that of a typical
commercial or laboratory environment. If the
user of the BioXp™ system requires continued
operation during power mains interruptions, it is
recommended that the instrument be powered
from an uninterrupted power supply or a battery.
Power frequency
(50/60 Hz) magnetic
field IEC 61000-4-8
3 A/m 3 A/m Power frequency magnetic fields should be at
levels characteristic of a typical location in a
typical commercial or laboratory environment.
NOTE: UTis the AC mains voltage prior to application of the test level.
Codex DNA, Inc.
9535 Waples Street, Suite 100
San Diego, CA 92121-2993 USA
858.228.4115
Technical assistance:
[e-mail]
© 2020 Codex DNA, Inc. All rights reserved.