CODEX DNA BioXp 9600 system User manual

1
BioXp™ 9600 system — User guide
Part number 43056 |Revision 1 |Eective 19Sep2022

Contents
LEGAL NOTICES 3
SAFETY INFORMATION 4
MANUAL SYMBOLS FOUND IN THIS GUIDE 4
MARKING SYMBOLS ON THE EQUIPMENT 4
SAFETY WARNINGS 5
EQUIPMENT SPECIFICATIONS 7
SYSTEM COMPONENTS 7
TECHNICAL SPECIFICATIONS 7
SITE PREPARATION AND INSTALLATION 8
SYSTEM SITE PREPARATION 8
GETTING TO KNOW THE BIOXP 9600 SYSTEM 8
HARDWARE OVERVIEW 8
INSTRUMENT PORTS AND POWER SWITCH 8
WASTE CHUTE 9
SETTING UP THE BIOXP 9600 SYSTEM 9
LEAVE THE BIOXP 9600 SYSTEM POWERED ON 9
ETHERNET SETUP 10
START THE BIOXP 9600 SYSTEM 10
CONFIGURE THE BIOXP 9600 SYSTEM 11
TEST THE NETWORK CONNECTION 11
CHANGE THE CLOCK 12
RUNNING THE BIOXP 9600 SYSTEM 12
OBTAINING BIOXP KITS 12
LOADING THE BIOXP DECK 12
INITIATING A BIOXP RUN 13
TRACKING PROGRESS DURING A RUN 13
CANCELLING A JOB DURING A RUN 14
TROUBLESHOOTING 15
BIOXP™ DECK INSPECTION ERROR 15
GENERAL TROUBLESHOOTING 16
CLEANING AND MAINTENANCE 16
CLEANING INSTRUCTIONS 16
CALIBRATION 17
PREVENTATIVE MAINTENANCE 17
REPAIR 17
LOADING AND SETUP 18
LOADING 18
USING THE BIOXP PLATE EXTRACTOR 23
WEEE COMPLIANCE 24

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Legal notices
Limited use label license Except as otherwise agreed in writing by our authorized representative, this product is for INTERNAL
RESEARCH USE ONLY AND NOT FOR HUMAN, ANIMAL, THERAPEUTIC OR DIAGNOSTIC USE. For
additional information about your rights under this research license, visit codexdna.com.
Limited warranty a) Codex DNA, Inc.’s entire liability and your exclusive remedy if the Product fails to conform to the
following warranty (a “Nonconforming Product”) shall be, at Codex DNA, Inc.’s sole option, either repair or
replacement of such Nonconforming Product, or, if neither is practicable, a refund of the fees paid for the
Product. The warranty for the repaired or replaced Product is limited to the scope and remaining duration
of the original warranty for the Nonconforming product or, if longer, for 30 days after the date of shipment
to you of the repaired or replaced Product. The warranty is contingent upon proper use of the Product in
accordance with the user guide and does not apply to any Product that is subjected to unusual physical
or electrical stress, misuse, neglect, improper testing or storage, modification or unauthorized repair or
upgrade. b) Codex DNA, Inc. warrants that the product, as delivered, will be free from defects in material
and workmanship for a period of one year from the date it is delivered to you (“the Warranty Period”).
c) This warranty gives you specific legal rights and you may have other legal rights which vary from
state to state. This warranty is non-transferable. d) OTHER THAN AS EXPRESSLY SET FORTH ABOVE,
Codex DNA, Inc. MAKES NO WARRANTIES, EXPRESS, STATUTORY, IMPLIED, OR OTHERWISE. NO
DISTRIBUTOR, AGENT OR EMPLOYEE IS AUTHORIZED TO MAKE ANY MODIFICATIONS, EXTENSIONS
OR ADDITIONS TO THIS WARRANTY. e) You shall indemnify, defend and hold harmless Codex DNA, Inc.
from any costs, expenses, damages, or other losses arising out of i) any warranty of greater scope or
duration than that set forth in this Codex DNA, Inc. Limited Warranty; and ii) failure to disclaim implied
warranties and limit remedies and liabilities, by and on behalf of Codex DNA, Inc.
Trademark information Gibson Assembly® is a registered trademark and BioXp™ and Oligo Vault™ are trademarks of
Codex DNA, Inc.
Patents Gibson Assembly® US patent numbers 7,776,532, 8,435,736, and 8,968,999.
Regulatory statement For research use only.
Statement of
proprietary information
Copyright in this work is vested in Codex DNA, Inc. and the document is issued in confidence for the
purpose only for which it is supplied. It must not be reproduced in whole or in part except under an
agreement or with the consent in writing of Codex DNA, Inc. and then only on the condition that this
notice is included in any such reproduction. No information as to the contents or subject matter of this
document or any part thereof arising directly or indirectly there from shall be given orally or in writing or
communicated in any manner whatsoever to any third party being an individual firm or company or any
employee thereof without the prior consent in writing of Codex DNA, Inc.
Terms and conditions IMPORTANT — This agreement (the “Agreement”) is a legal agreement between you and
Codex DNA, Inc. By installing or otherwise using the BioXp™ 9600 system (referred in this agreement as
the “Product”), you agree to be bound by the terms of this Agreement. If you do not wish to be bound by
the terms of this Agreement, do not use the Product and promptly return the unused, unopened Product
to the place of purchase for a full refund. The user is prohibited from reverse engineering the software or
output files generated from the BioXp™ 9600 system.
Limitation of liability CODEX DNA, INC.’S LIABILITY ARISING OUT OF OR RELATING TO A PRODUCT SHALL NOT EXCEED
THE AGGREGATE AMOUNTS YOU PAID TO CODEX DNA, INC. FOR THE PRODUCT. IN NO EVENT
WILL CODEX DNA, INC. BE LIABLE FOR LOST USE, PROFITS, REVENUE, COST OF PROCUREMENT
OF SUBSTITUTE GOODS, OR ANY OTHER SPECIAL, INDIRECT, RELIANCE, INCIDENTAL, OR
CONSEQUENTIAL DAMAGES, HOWEVER CAUSED AND UNDER ANY THEORY OF LIABILITY. THE
FOREGOING LIMITATIONS SHALL APPLY REGARDLESS OF WHETHER CODEX DNA, INC. HAS BEEN
ADVISED OF THE POSSIBILITY OF SUCH DAMAGES AND NOT WITHSTANDING THE FAILURE OF
ESSENTIAL PURPOSE OF ANY LIMITED REMEDY.
Disclaimer The material in this manual is for informational purposes only and is subject to change without prior
notice at any time. Codex DNA, Inc. and/or its aliates assume no responsibility for any errors that may
appear in this document.
Indications for use The BioXp™ 9600 system is a device which is intended for the processing of DNA materials.
The system is not approved for use other than that stated above.

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Safety information
Manual symbols found in this guide
This manual describes the use and maintenance of the Codex DNA BioXp™ 9600 system. Read this manual completely before
putting the instrument into service. The following icons are used in this manual:
General Warning
Indicates a warning concerning operations that may lead to personal injury or potential
damage to the instrument if not performed correctly.
Electrical Safety warning
Failure to follow these instructions can cause harm to operators or subjects.
Important note
Failure to follow these instructions can lead to damage of the system or unexpected results.
Marking symbols on the equipment
Electrical Safety warning
Indicates a warning concerning operations that may lead to personal injury or potential
damage to the instrument if not performed correctly.
DO NOT MOVE
BY HAND
CAUTION
Caution, hot surface, do NOT touch
Warning: Flammable Material
Alternating current
Waste Electrical and Electronic Equipment (WEEE) directive
Do not dispose symbol. For more information, see Equipment disposal and WEEE
compliance.
Consult instructions for use.

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Safety warnings
Installation
• The BioXp™ 9600 system is very heavy, 210lbs (95kg). Do not attempt to lift or move the instrument
without the assistance and supervision by colleagues. Be certain to use appropriate moving equipment
and proper lifting techniques (including but not limited to making certain that you have a secure grip
on the instrument before lifting, keeping your spine in a neutral position while lifting with your legs, and
coordinating lifting and moving movements with all appropriate personnel). Improper lifting can cause
permanent back injury. The BioXp 9600 is fitted with four lifting straps at each corner. It’s recommended
to use four people to lift the system and for each to loop their wrist through the strap to ensure a firm
grip on the strap before lifting. If possible, utilize a forklift or other lifting device to bring the crate to a
comfortable level before lifting the instrument o the lower crate support.
• Use this product only as specified. If the instrument is used in a manner not specified by Codex DNA,
the protection provided by the instrument may be impaired, resulting in personal injury or damage to the
instrument.
Ground circuit protection
• Check the mains electricity supply plug, voltage, and frequency to confirm that the supply corresponds to
the values shown on the instrument label.
• Ensure that the supply of power to the instrument is via a socket fitted with a protection device, such as a
circuit breaker of the correct rating, to provide automatic power cut-o in the event of an insulation fault.
• Confirm that the mains wiring is grounded. Codex DNA declines all responsibility for any damages due to
instrument non-grounding.
Main power switch
Do not position the instrument such that it is dicult to operate the main power switch.
Hazardous chemical warning
• Chemicals used with the instrument may be hazardous.
• Always wear protective gear including safety goggles, gloves, and a lab coat when handling chemicals.
• Exercise caution when handling flammable liquids.
• Take precautions, including but not limited to installing proper ventilation systems, to ensure that the
BioXp™ system laboratory is safe and that operators of the instrument will not be exposed to hazardous
levels of harmful chemicals.
• Follow all national, state, and local health and safety regulations and laws.
• Use proper waste disposal in accordance with all relevant regulations.
• Refer to applicable material safety data sheets (MSDSs).

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Flammable liquids: Instrument warning
• Exercise caution when using flammable liquids in the BioXp™ system to minimize the risk of fire.
• Do not use any flammable liquids in the instrument except for the liquids required in the included
procedure.
• Provide adequate ventilation for the instrument to prevent the accumulation of flammable vapors.
• All sources of ignition, such as open flame or electrical spark, are not permitted near the instrument or in
areas where flammable vapors may travel.
• Ensure that the instrument, all neighboring equipment, and users of the instrument are appropriately
grounded to minimize the generation of static electricity.
• Do not use the ethanol reservoir if it is cracked or the lid is damaged and not sealing correctly.
Flammable liquids: Solution handling warning
• Keep flammable liquids in covered containers when not in use.
• Provide means to promptly and safely dispose of any flammable liquid leaks or spills.
• Do not transfer liquids using air pressure.
• Read applicable MSDSs.
• Store flammable liquids in cool, well-ventilated areas away from corrosives, oxidizers, and ignition sources.
• Label containers and cabinets as “flammable materials” where applicable.
• Use only approved safety vessels for flammable liquid storage.
• Ground and bond flammable liquid containers to prevent static charge buildup.
• Never pour flammable liquids down a drain or sink.
• Dispose of empty flammable containers in an approved manner.
• Remove any unused flammable liquids from the instrument after completion of a run.
Electrical safety warning
• Do not attach the power cord to an extension cord or to a multiple portable socket. Doing so may
compromise shielding and/or grounding.
• This equipment should not be used adjacent to or stacked with other equipment. If it becomes necessary
to use the BioXp™ system under these conditions, the unit should be observed to verify normal operation
in this condition.
• Use only grounded mains outlets to supply instrument.
• Do not remove any panels. Panels should be removed only by trained service personnel.
• The AC power cord is the primary disconnection device for the instrument. Ensure that the point of
connection is easily accessible and free of any obstructions.
Important warning: Moving parts
• Do not override the door lock. When the door is locked, the moving parts of the instrument are
operational. Never attempt to physically restrict movement of instrument components.
• Interfering with moving parts may cause personal injury. Keep hands clear of moving parts while
operating the instrument.
• Do not move or shift the instrument during operation.
• If the instrument shows evidence of corrosion or wear, do not attempt to manipulate or tamper with
components of the instrument.

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Important warning: Disconnecting the instrument
• Disconnect power to the system before cleaning or performing maintenance.
• DO NOT remove any panels; they should be removed only by qualified service personnel.
• Do not disassemble unit.
Equipment specifications
Covers and exterior components which the operator may come in contact with during routine maintenance
or calibration, shall operate at a voltage no greater than 25 V AC or 60 V DC.
System components
• Use of parts or materials other than those supplied with the instrument can degrade system performance
and may cause safety hazards. Use only Codex DNA approved or supplied components.
• Use only Codex DNA supplied power cord. Use of other power cords may compromise electrical protection
and could create a hazard.
Components of the BioXp™ system
• BioXp™ 9600 system
• Power cord
• RJ45 ethernet cable
• Reagent and recovery chiller thermal covers
• Ethanol reservoir
• MegaStrip holder
• Oligo Vault Removal Tool
Technical specifications
Product Name BioXp™ 9600 System
Catalog Number BX9600-01
Description Automated synthetic biology workstation
Power input voltage 100 – 240VAC (50Hz /60Hz)
Power input current 10 A max
Operating temperature range 16 to 30 °C
Storage temperature range 0 to 45 °C
Operating and storage humidity range 10 to 90% (non-condensing relative humidity)
Operating altitude Sea level to 1828 m [6,000 ft.]
Water ingress Non-immersion; protection for damp wipe only IP10.
Weight 95 kg [210 US lbs]
Dimensions (W × D × H) 122 × 76 × 92 cm [48 × 30 × 36 in] with hood open

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Site preparation and installation
System site preparation
For details on site preparation, safety precautions, uncrating, setup, and powering up, see the BioXp™ 9600 system remote
installation and sign-o technical guide.
Getting to know the BioXp 9600 system
Hardware overview
Figure 1. Front view of the BioXp 9600 System Figure 2. Inside Deck of the BioXp 9600 system
Figure 3. Example of the deck layout at the beginning of a run.
1. Temporary Plate and Lid Storage #1
2. Temporary Plate and Lid Storage #2
3. Output Chiller Cover Storage Location
4. Thermal Cycler
5. Magnetic Separation Station
6. Output Chiller
7. Reagent Chiller Cover Storage
8. Mega Strip Holder
9. Reagent Chiller
10. Input Plate Stack #1
11. Input Plate Stack #2
12. Tip Tray Storage #1
13. Tip Tray Storage #2
14. Tip Tray Storage #3
15. Tip Tray Storage #4
16. Strip Well Holder
17. Waste Chute
18. Ethanol Trough
19. Tip Drawer
Instrument ports and power switch
Location

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Label no. Port/inlet Description
1USB
The USB port is for instrument
diagnostics and is for authorized
service personnel use only
2Ethernet Connection to the Codex DNA
server for system communication
3Fuse AC inlet fusing
4Power
switch
Switch power to the instrument on
or o. Note: Leave the instrument
on continuously.
5Power
inlet Power the instrument
Figure 4. Power on/o switch
and communications ports
Waste chute
Proper installation of the waste chute on the deck is shown in the image below. The waste chute is removable with three thumb
screws and can be lifted ‘up and out’ of the BioXp system for cleaning. Before beginning a run on the BioXp system verify that
the waste chute is installed and that the thumbscrews have been properly tightened.
Setting up the BioXp 9600 system
Leave the BioXp 9600 system powered On
3
4
2
5
1

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We recommend leaving the instrument on continuously. Only turn the instrument o when necessary, such as when moving the
instrument to another location. If the instrument must be turned o, toggle the power switch to the O position.
Ethernet setup
The BioXp system requires an internet connection to load jobs and to aid during service. Prior to connecting the instrument to
the internet, be sure to test the connection. Connect the provided RJ45 ethernet cable to the ethernet port — located on the
side of the unit — to an available ethernet wall jack.
In order for the BioXp system to communicate with the Codex DNA server, you must open the 80/443 ports through your
network. In addition, the BioXp system will be making an outbound SSL connection (HTTPS/SSL) to logmein.com, codexdna.com
and drive.google.com. Unblock or whitelist these sites.
During remote diagnostic testing or software updates, a Codex service representative may connect to the BioXp system using
logmein to perform service operations. These updates will be done while the system is not in use.
Start the BioXp 9600 system
1. Turn the instrument on using the power switch located on the side of the
instrument.
2. Close the instrument door and tip drawer. The BioXp system will initiate
a self-check process to verify the correct functionality of motion control
and other internal systems of the instrument. If the instrument is unable
to perform a successful self-check it will alert the user to the problem and
will prevent the instrument from operating until the problem has been
addressed. The self-check process has been carefully developed to ensure
that the BioXp system is operating within optimal calibration ranges and that
all systems are functioning correctly before initiating a run.

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Configure the BioXp 9600 system
1. Press the Configuration gear icon in the lower left corner
2. Press Network
Test the network connection
3. Press Test connection
If the system displays Network connection working normally,
the BioXp system is ready for use. If you are ready to load the
instrument and begin a run, refer to the loading map included
with your kit components.
If the system displays Unable to connect to the cloud contact
Codex DNA customer service at [e-mail] for
assistance in resolving the connection.

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Change the clock
Press Date and time
Use the up and down arrows to set the time and date
manually. Then press OK.
Notes:
• Time is displayed in hours : minutes : seconds and the date
is displayed in month/day/year.
• Check DST to automatically update clock to daylight saving
time.
Running the BioXp 9600 system
Obtaining BioXp Kits
To order custom BioXp application kits and reagents, visit the Codex DNA myBioXperience portal at codexdna.com. After
creating an account, you will be able to upload sequences and place an order. For assistance with placing orders through
myBioXperience, please reach out to [e-mail] or 858-526-3080.
Once you’ve ordered and received your BioXp application project, running the BioXp system is as easy as loading the deck and
pressing Start.
Loading the BioXp deck
Please refer to the site readiness guide for details on necessary equipment to prepare your BioXp kit reagents for loading.
Setting up the deck of your BioXp is unique to your
application. We provide a detailed Loading map and checklist
with each of your custom kits - simply refer to this document
when you are ready to run your BioXp.
Press the Next-> button and the system will initialize.

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Initiating a BioXp run
1. Once the deck and tip drawer is loaded, confirm that all
components are securely seated.
2. Close the door and tip drawer.
3. Ensure there is a suitable waste container positioned in
front of the waste chute.
4. Press the Play button. The run will initiate.
Tracking progress during a run
Time to completion
During a run, the instrument will display the projected time
remaining for the run (in hours : minutes : seconds) The start
time and the estimated time of completion.
BioXp run completion
1. When the BioXp run is complete, the time of completion
and the time since the completion will display along with a
Unlock button.
2. Pressing the Unlock button will display a progress screen
as the system prepares for opening.
Once the door unlatches and the screen displays the
message to open the door you should refer to your Loading
map and checklist for general information on run breakdown
and final product locations.
Refer to the Plate map for the location of each of your custom
constructs. Both of these documents are provided with your
BioXp kit™.

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Cancelling a job during a run
IMPORTANT! You will not be able to reuse reagents if you cancel a job after the run has initiated and the reagent plate seal has
been broken. Do not cancel a run unless absolutely necessary.
1. If you need to cancel a job during a run, press the ‘X’ icon
at the bottom of the running screen. This will display the
cancel job screen.
2. Sliding the Trash icon to the right before the progress
indicator completes its travel will cancel the job. The latch
will open and you will be able to remove the materials
from the deck.
If you do not slide the trash icon to the right before the progress indicator completes or if you press the Check icon then the
running screen will redisplay and the job will continue.

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Troubleshooting
BioXp™ deck inspection error
After you load the deck and close the instrument door and
start the run, the instrument performs a deck inspection,
checking the location and orientation of the recovery plate,
ethanol reservoir, reagent strip handles, reagent strips, tips,
and system robotics.
If any anomalies are detected, the instrument displays a deck
inspection failure message, similar to the screen shown below.
If the system displays a deck inspection failure message, see
the following table for recommendations.
Potential Issues Possible solution and recommendations
Deck inspection detected anomalies with one (or more) of
the following:
• Plate found in purification station
• Recovery plate not found or is rotated
• Ethanol reservoir is missing or misplaced
• Strip handle not closed
• Not enough tips or other consumables loaded
• Incorrect sequence of input plate colors in the plate stack.
Press Stop and fix
• Open the door and correct the deck inspection issue.
• After fixing the error, close the door to initiate a second
deck inspection.
• If the deck inspection error message persists after you
have corrected any issues, email: [e-mail]
Error messages
If you observe an error screen during operations, follow the screen instructions. If directed or if the problem persists, make a
note of the screen and email [e-mail]

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General troubleshooting
Codex DNA technical services: [e-mail]
Potential Issues Possible solution and recommendations
Touchscreen does not light up • Confirm that the power switch is in the ON position.
• Confirm that the instrument is plugged into a working
electrical outlet.
• Verify that the plug is securely connected to the side of
the instrument.
• Contact Codex DNA technical services.
Power failure occurs during the run
Contact Codex DNA technical services.
Diculty operating the touchscreen
Cleaning and maintenance
Cleaning instructions
• Clean the exterior of the BioXp system with standard cleaners and a damp cloth.
• Clean the internal surfaces periodically using a mild detergent or 70% ethanol. Spray the cleaner onto a cloth and then use the
damp cloth to wipe instrument surfaces. Do not spray any cleaners directly into the instrument.
• You can remove the waste chute for cleaning by removing the three retaining thumbscrews.
Do not use abrasive or caustic cleaners on internal or external instrument surfaces.
Spills
Take every precaution to ensure that no liquids are spilled in the instrument. Wipe up accidental spills in and around the
instrument immediately. If ethanol spills, dispose of ethanol-saturated towels according to your institutional guidelines.
In the event of hazardous material spillage
In the event of spillage of a hazardous material, the BioXp system user is responsible for decontamination of the hazardous
material on or inside the instrument.
Before using a cleaning or decontamination method other than a recommended cleaner, contact Codex DNA to verify that the
proposed decontamination method will not damage the equipment.

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Calibration
Calibration of the motion control system is performed by the manufacturer. Yearly verification of the system calibration is
recommended for optimal performance. The instrument will perform a self check each day. A log of the self check is stored on
the system and may be used by Codex DNA service technicians should any issue arise. Keeping the BioXp system connected to
the internet ensures optimal use and analysis of the calibration log.
Preventative maintenance
The BioXp system requires periodic cleaning and lubrication of the motion system. This procedure is ideally performed annually
and is included in the optional service contract. Contact customer service for warranty and service contract inquiries.
Repair
Each BioXp system has been manufactured and calibrated to specifications. Do not attempt to disassemble any part of the
system. Any such action will void the product warranty. In the event you should experience a product failure, call Codex DNA
technical services at 858.228.4115.

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Loading and setup
Loading
Proper loading of the BioXp system begins with gathering the provided the materials received in your reagent kit.
To start, open the hood and examine the interior of the BioXp system – make sure any materials or waste plates from a prior run
have been removed before you begin loading the new consumables and reagents into the BioXp system. Refer the image on
page 8 of this document - you will need to verify that all locations have been emptied of any materials or empty tip trays from
the prior run. Verify that the waste chute is not blocked and any waste receptacle under the waste chute is empty and ready to
receive the waste from the next run.
NOTE: Not all loading steps described below are applicable for every protocol type. These are general guidelines to provide
guidance on the loading process. Some protocols have unique preparation steps such as thawing on ice or centrifugation before
loading. Be sure to follow the guidelines in the specific loading guide provided with the kit being loaded.
Installing the Oligo Vault™ into the Thermal Cycler (location 4)
The Oligo Vault™ is keyed and will only fit into the thermal cycler
in one orientation. The keyed component is in the top left
corner of the Oligo Vault once placed into the thermal cycler,
gently press the Oligo Vault into the thermal cycler to verify
proper placement, ensure the aluminum cover is centered in the
vault – see right.
Installing the DNA Synthesis Plate into the Reagent Chiller
(location 9)
The DNA Synthesis Plate (NOTE: some loading instructions
refer to this as the RNA Synthesis Plate) is keyed on the left front
corner and ‘snaps’ into the reagent chiller. Once placed into the
chiller be certain to gentry press down on the top of the plate to
seat it into the retention clamps – see right.
Installing frame strips into the Strip Well Holder (location 16)
If your protocol includes frame strips, install them into the
strip well holder on the deck as seen below. There are two
retention clamps that must be opened before a frame strip
can be installed. Pay special attention to align the small hole
in the frame strip with its corresponding positioning pin in the
strip well holder. The strip positions will be specified in the
loading instructions provided for the protocol of your order. The
positions are numbered from 1 to 4 (left to right) of the holder.
For example, we are loading this framestrip into location 2 of the
strip well holder:

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Press the framestrip into the holder and verify the location of
the positioning pin and corresponding hole.
The clamp assembly of the framestrip holder snaps into place to retain the framestrip. After installing all the framestrips
necessary for your protocol – gently close the clamp assembles to retain the framestrips. Due to the low volume of reagent in
the frame strips, this must be done carefully, without disrupting reagents pooled in the bottom of the tubes.
Installing Ethanol Trough (location 18)
Skip if your protocol does not require the ethanol trough for
washing step.
The trough is easily removed by lifting it free from the deck (it
is retained magnetically) and then unsnapping the removable
‘pierceable ‘cover to allow for ethanol to be poured into the
trough.
The trough can hold up to 60ml. Fill the trough as directed
by the loading instructions with the proper amount and
concentration of ethanol for the protocol you are running and
restore the pierceable lid – this should snap onto the trough
and be securely retained. Once secured, place the whole
assembly back onto the deck in the holder.
NOTE: there is no specific orientation for the tough assembly.
and it is not recommended to fill the ethanol trough while
installed into the BioXp system. The cover for the trough must
be fully snapped onto the trough or it may become dislodged
during a run causing a failure
Do not attempt to fill the ethanol trough while still installed on the deck of the BioXp! Fully remove the trough and
fill on an appropriate lab bench or fume hood.

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Installing Mega Strip Holder and Mega Strips (location 8)
If your protocol requires Mega Strips for reagent delivery these are installed into the Mega Strip Holder which is a removable
component and can be placed (along with the mega strips) into a centrifuge for preparation – then placed into the mounting
system permanently installed on the deck of the BioXp system.
The bottom left corner of the mega strip holder is keyed and when properly mounted into the BioXp system the spring clips on
the right and left of the holder will snap the holder into the retaining holder. Each of the locations is labeled from 1 – 6. The load-
ing instructions will designate which location the Mega Strip(s) will need to be inserted to.
NOTE: To more easily remove the mega strip holder, depress one (or both) of the spring clips and lift the mega strip holder from
the mounting system.
The mega strips are keyed on the ends and can only be inserted in one orientation (wide tab) to the front of the BioXp system.