Curiox Laminar Wash HT1000 User manual

HT1000 User Manual (IN-CL-UD-3 Rev.6) © 2020 Curiox Biosystems Pte. Ltd. For Research Use Only
Laminar Wash™ HT1000 Station
96 User Manual
Copyright © 2020 Curiox Biosystems Pte. Ltd. All rights reserved.
Curiox Biosystems Pte. Ltd.
2 Woodlands Spectrum, #03-10
Woodlands Sector 1, S(738068)
T: [phone]
F: [phone]
E: [e-mail]
W: www.curiox.com
Laminar Wash HT1000 User Manual
Revision 6, Sep 2020

HT1000 User Manual (IN-CL-UD-3 Rev.6) © 2020 Curiox Biosystems Pte. Ltd. For Research Use Only
The information contained in this manual is believed to be accurate, but Curiox Biosystems Pte.
Ltd. accepts no liability for any errors it contains, and reserves the right to alter specifications
without notice.
Trademarks
Curiox, the Curiox logo, and Laminar Wash are trademarks or registered trademarks of Curiox
Biosystems. Other marks appearing in these materials are marks of their respective owners.

HT1000 User Manual (IN-CL-UD-3 Rev.6) © 2020 Curiox Biosystems Pte. Ltd. For Research Use Only
| i
Table of Contents
Chapter 1:
General Information
. . . . . . . . . . . . . . . . . . 1
General Information . . . . . . . . . . . . . . . . . . . . . . 2
Introduction to the Laminar Wash HT1000. . . .2
Technical Specifications . . . . . . . . . . . . . . . . 3
Chemical Compatibility . . . . . . . . . . . . . . . . . . . . 4
Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
User Attention Notifications . . . . . . . . . . . . 5
Chemical Hazards . . . . . . . . . . . . . . . . . . . . . 5
Chemical Waste Hazards . . . . . . . . . . . . . . . 6
Material Safety Data Sheets . . . . . . . . . . . . 6
Instrument Safety Labels. . . . . . . . . . . . . . . . 6
Instrument Safety Features . . . . . . . . . . . . . 7
Safety Precautions . . . . . . . . . . . . . . . . . . . . 7
General Precautions . . . . . . . . . . . . . . . . . . . 8
Prior to System Operation . . . . . . . . . . . . . . 8
CE Mark . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Customer Service and Technical Support . . . . . 10
Chapter 2:
Functional Description
. . . . . . . . . . . . . . . 11
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Functional Description . . . . . . . . . . . . . . . . . . . . 12
Wash Cycle Process . . . . . . . . . . . . . . . . . . . 14
Chapter 3:
Setup
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15
Unlocking and Installation . . . . . . . . . . . . . . . . 15
Unlocking . . . . . . . . . . . . . .. . . . . . . . . . . . 16
Installation . . . . . . . . . . . . . . . . . . . . . . . . . 19
Buffer Inlet Bottle Cap Installation . . . . . . 20
Preparation for Operation . . . . . . . . . . . . . . . . .21
Chapter 4:
Operation
. . . . . . . . . . . . . . . . . . . . . . . . . .23
Before Operating the HT1000 . . . . . . . . . . . . . 24
Operational Safety . . . . . . . . . . . . . . . . . . . . . .24
Plate Detection . . . . . . . . . . . . . . . . . . . . . . .24
Power Supply . . . . . . . . . . . . . . . . . . . . . . . . .24
Start / Pause . . . . . . . . . . . . . . . . . . . . . . . . ..24
Machine Homing. . . . . . . . . . . . . . . . . . . . . . 24
Spill Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Safety Precautions. . . . . . . . . . . . . . . . . . . . . 25
Operational Mode . . . . . . . . . . . . . . . . . . . . . . . . 25
LEDs and Buttons. . . . . . . . . . . . . . . . . . . . . .25
Wash Cycle . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Service Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
LEDs and Buttons. . . . . . . . . . . . . . . . . . . . . .27
Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . .28
Shutdown Procedure . . . . . . . . . . . . . . . . . . .29
Chapter 5:
Troubleshooting and Maintenance.
. . 30
Troubleshooting Checklist . . . . . . . . . . . . . . . . . .31
Technical Support . . . . . . . . . . . . . . . . . . . . . . . . .31
Maintenance Schedule . . . . . . . . . . . . . . . . . . . . .32
Appendix
A:
Acknowledgement of Decontamination. . . . ..33
Acknowledgement of Decontamination Form . .34
Appendix
B:
Purchase Information and Feedback . . . . . . .35
Purchase Information and Feedback Form. . . . .36

HT1000 User Manual (IN-CL-UD-3 Rev.6) © 2020 Curiox Biosystems Pte. Ltd. For Research Use Only
| 1
Chapter 1:
General Information
Chapter Overview
• General Information
• Introduction to the Laminar Wash HT1000
• Chemical Compatibility
• Safety
• CE Mark
• Customer Service and Technical Support

HT1000 User Manual (IN-CL-UD-3 Rev.6) © 2020 Curiox Biosystems Pte. Ltd. For Research Use Only
2 | Chapter 1: General Information
General Information
In general, this user manual has been written for the purpose of providing technical, installation,
operating and troubleshooting information to the operators of the Laminar Wash™ HT1000 Station. The
content of this manual includes:
• How to set up and operate the HT1000
• The principle of operation and modes of function for the HT1000
• Safety features of the HT1000 and precautions to ensure safe operation
• Troubleshooting procedures and maintenance
Introduction to the Laminar Wash HT1000
The Laminar Wash solution enables sample preparation for multi-color flow cytometry and bypasses
the need of centrifugation for washing cells in suspension. The Laminar Wash technology is designed
to maintain samples in a 96-droplet based format instead of using a conventional microwell with
walls. It is a centrifuge-less processing method using the unique wall-less features of the Laminar
Wash technology combined with a fully automated washer. The Laminar Wash HT1000 generates a
laminar flow for each drop on the Laminar Wash 96-well Plate via dual-nozzle action, with one nozzle
dispensing liquid into the drop and the other nozzle aspirating liquid. The Laminar Wash workflow is
superior over the traditional centrifugation method.

HT1000 User Manual (IN-CL-UD-3 Rev.6) © 2020 Curiox Biosystems Pte. Ltd. For Research Use Only
Technical Specifications | 3
Technical Specifications
Description Specification
Physical
Dimensions 310 mm H x 262 mm W x 302 mm D
W
eig
h
t
1
4
kg
Elect
r
i
ca
l
V
o
l
ta
g
e
R
equi
r
eme
n
t
1
00
-
2
4
0
V
Environmental
Operating Temperature
Ran
g
e
15 - 28 °C
O
pe
r
a
ting
Humidity
<
80%,
non
-
c
o
n
densing
Operatio
n
Plate Type
Laminar Wash
Plat
e
in 9
6
-
well
f
orm
a
t
Capacity
1 plate per operation
Perf
ormance
Flow rate at nozzle
5
-
20µl/s
Volume capacity
80µl per nozzle
Wash sequence
96 wells simultaneous washing
Dilution factor per cycle
Approx. 3.5 times
Bulk flow rate for
priming
Approx. 300ml/ min
User Interface
Display
A single plus a double
-
digit display,
4 status indic
ating LED
Input control
4 input buttons
Number of washes per
cycle
1x to 9x selectable
External Interface
1x inlet
A connector for incoming
fresh wash buffer
1x outlet
A connector for outgoing waste wash buffer
1x RS232
A plug
-
in for
up
dating operati
ng parameters

HT1000 User Manual (IN-CL-UD-3 Rev.6) © 2020 Curiox Biosystems Pte. Ltd. For Research Use Only
4 | Chapter 1: General Information
Chemical Compatibility
The components exposed to fluids and reagents are composed of materials which were selected
to be resistant against common chemical substances. However, some common disinfectants
should not be used when decontaminating the HT1000. Table 1-1 on page 4 lists the material
composition of the main components of the HT1000, and shows some reagents which do not pose
issues when in contact with these components. However, some reagents are not compatible with
the component materials, and prolonged contact should be avoided to prevent corrosion and
damage.
Component Material Approved
Chemicals
Incompatible
Chemicals
Internal base
structure Stainless Steel (304)
Ethanol, Benzene,
Chloroform, Acetaldehyde,
Propylene Glycol,
Isopropanol, Formaldehyde,
Phenol, Grease, Potassium
Permanganate, Hydrogen
Peroxide
Hypochlorite bleach,
Sulfuric Acid
Some minor
components of
main body
Delrin
(Polyoxymethylene)
Ethanol, Benzene, Soap
Solutions, THF,
Formaldehyde, Propylene
Glycol, Isopropanol,
Potassium Permanganate
Acetic acid, Grease,
Ketones, Ozone,
Phenol, Ammonia,
Hypochlorite bleach,
Iodine, Hydrogen
Peroxide, Phosphoric
Acid, Sodium Hydroxide
(>50%)
Internal
structural parts
Aluminum
Ethanol, Benzene,
Propylene Glycol,
Isopropanol,
Formaldehyde, Ozone,
Grease, Phenol, Hydrogen
Peroxide
Hypochlorite Bleach,
Soap Solutions, Sulfuric
Acid
,
Potassium
Permanganate,
Phosphoric Acid
External
structure
Table 1-1: Chemical compatibility between the component materials in the HT1000, including common
reagents and disinfectants. (Adapted from Ingersoll Rand Industrial Technologies, 2008 & K-mac Plastics).

HT1000 User Manual (IN-CL-UD-3 Rev.6) © 2020 Curiox Biosystems Pte. Ltd. For Research Use Only
Safety | 5
Safety
User Attention Notifications
Several user attention phrases are used throughout this manual. Each phrase should draw the
following level of attention from the user:
NOTE Points out useful information.
IMPORTANT Indicates information necessary for proper instrument operation.
CAUTION Cautions users regarding potentially hazardous situations in regard to user
injury or damage to the instrument if the information is not heeded.
!WARNING! Warn users that serious physical injury can result if warning precautions are
not heeded.
Chemical Hazards
!WARNING! CHEMICAL HAZARD
Some chemicals used can be potentially hazardous, and can cause injury or illness.
• Read and understand the Material Safety Data Sheets (MSDSs) provided by the chemical
manufacturer before you store, handle, or work with any chemicals or hazardous
materials.
• Minimize contact with and inhalation of chemicals. Wear appropriate personal
protective equipment when handling chemicals (e.g., safety glasses, gloves, or
clothing). For additional safety guidelines consult the MSDS.
• Do not leave chemical containers open.
• Check regularly for chemical leaks or spills. If a leak or spill occurs, follow the
manufacturer's cleanup procedures as recommended on the MSDS.
• Comply with all local, state/provincial, or national laws and regulations related to
chemical storage, handling, and disposal.

HT1000 User Manual (IN-CL-UD-3 Rev.6) © 2020 Curiox Biosystems Pte. Ltd. For Research Use Only
6 | Chapter 1: General Information
Chemical Waste Hazards
• Read and understand the Material Safety Data Sheets (MSDSs) provided by the
manufacturers of the chemicals in the waste container before you store, handle, or
dispose of chemical waste.
• Minimize contact with chemical waste. Wear appropriate personal protective equipment
when handling chemicals (e.g., safety glasses, gloves, or clothing).
• Use precaution when emptying the waste bottle.
• Dispose of waste bottle contents in accordance with good laboratory practices and local, state/
provincial, or national environmental and health regulations.
Material Safety Data Sheets
Some chemicals used with the HT1000 may be listed as hazardous. Warnings are displayed on the
labels of all chemicals when hazards exist.
MSDSs provide users with safety information needed to store, handle, transport and dispose of
the chemicals safely. Curiox recommends updating laboratory MSDS records periodically.
Material Safety Data Sheets for Curiox reagents are available upon request by calling 650 226-
8420 in the US or [phone] outside the US. Otherwise call the chemical manufacturer
directly or visit their web site.
Instrument Safety Labels
The following safety label is located on the HT1000. The label displays a safety alert symbol
indicating a potential safety hazard.
Symbol Description
Pinch point hazard. Keep hands clear.
Separate collection for electrical and electronic equipment.

HT1000 User Manual (IN-CL-UD-3 Rev.6) © 2020 Curiox Biosystems Pte. Ltd. For Research Use Only
Instrument Safety Features | 7
Instrument Safety Features
Plate Detection
The HT1000 detects the presence of a Laminar Wash 96-well Plate. The machine will not begin
operation if a plate is not detected on the feeder platform.
Power Supply
The universal power adapter is able to accommodate electrical power in the range of 100 - 240 V.
The resultant output to the HT1000 power jack is 24 V DC.
Start / Pause
Activating the “Start / Pause” button in the middle of a washing cycle will cause the machine to
immediately freeze. The machine goes to home position when the button is subsequently
activated again.
Machine Homing
In the event power to the machine is cut off in the middle of a washing cycle and subsequently
switched on again, the machine will go to home position.
Spill Tray
The spill tray sensor detects excess liquid in the tray to prevent it from spilling onto the
machine’s interior. When this happens, the machine LED light turns red and error code “E31” is
prompted on the machine display panel. User may only access the draining button during this
time.
Safety Precautions
• At the end of a wash process, remove the plate from the feeder platform immediately
to prevent the wells from drying, stressing cells and/or deteriorating biological
integrity.
• Keep the area around the power supply free from liquid.
• During active operation, keep your hands off the instrument other than using the display
panel.
• If there is an unexpected error, reset the instrument by turning the power switch off
and back on.

HT1000 User Manual (IN-CL-UD-3 Rev.6) © 2020 Curiox Biosystems Pte. Ltd. For Research Use Only
8 | Chapter 1: General Information
General Precautions
• Do not use any other plate except the specified Laminar Wash 96-well Plate with the HT1000.
• Do not load more than one Laminar Wash 96-well Plate in the HT1000 at a time.
• Use only the supplied power adapter cord for electrical supply to the unit.
• Do not allow particles larger than 200 µm to enter any of the liquid tubes.
• Be careful not to spill liquid onto the interior of the HT1000.
• Always perform a cleaning cycle with an appropriate cleaning solution at the end of
an experiment.
• Keep the original packaging material in case the unit should ever need to be shipped.
• Do not attempt to open or remove the instrument casing or motor parts. Doing so will void
the calibration and warranty and may cause permanent damage to the instrument.
• Contact only qualified Curiox personnel for servicing of the HT1000.
Prior to System Operation
Ensure that all users of the HT1000 have:
• Received instruction in general safety practices for laboratories.
• Received instruction in specific safety practices for the instrument.
• Received instruction on handling of biohazards if biohazardous materials are to be used on
the system.
• Read and understood all related MSDSs.
CAUTION
Avoid using the HT1000 in a manner not specified by Curiox. While the system has been
designed to protect the user, this protection may be impaired if the instrument is used
improperly.

HT1000 User Manual (IN-CL-UD-3 Rev.6) © 2020 Curiox Biosystems Pte. Ltd. For Research Use Only
CE Mark | 9
CE Mark
Based on the testing described below and information contained herein, this instrument bears
the CE mark.
Directive 2014/30/EU Electromagnetic Compatibility
This device has been type-tested by an independent, accredited testing laboratory and found to
meet the requirements of EN 61326-1 for Emissions and Immunity.
Verification of compliance was conducted to the limits and methods of the following:
1. EN 61326-1: 2013 Emissions
a. Harmonics Current Emission (Class A)
b. Voltage Fluctuation/ Flicker
c. Conducted Emission (Group 1 Class B)
d. Radiated Emission (Electric Field) (Group 1 Class B)
2. EN 61326-1: 2013 Immunity
a. Electrostatic Discharge Immunity
b. RF Radiated Immunity
c. Electrical Fast Transient/ Burst Immunity
d. Voltage Surge Immunity
e. Conducted Disturbance Immunity
f. Voltage Dips & Interruptions Immunity
Directive 2014/35/EU Low –Voltage Device
This device has been verified and found to meet the requirements of Directive 2014/35/EU
“electrical electronic equipment designed for use within certain voltage limits”.
Directive 2011/65/EU Restriction On the use of Hazardous Substances (ROHS 2)
This device has been verified and found to meet the requirements of Directive 2011/65/EU
“restriction on the use of certain hazardous substances in electrical and electronic equipment”.
Directive 2012/19/EU Waste Electrical and Electronic Equipment (WEEE)
Dispose of the device according to Directive 2012/19/EU, on “waste electrical and electronic
equipment (WEEE)” or local ordinances.

HT1000 User Manual (IN-CL-UD-3 Rev.6) © 2020 Curiox Biosystems Pte. Ltd. For Research Use Only
10 | Chapter 1: General Information
Customer Service and Technical Support
Telephone
[phone] (US)
[phone] (Singapore)
Fax
+ 1 650 590 5406 (US)
[phone] (Singapore)
E-mail
[e-mail]
Web
www.curiox.com
Addresses
Curiox Biosystems, Inc.
735 Industrial Road, #109
San Carlos, CA 94070
USA
Curiox Biosystems Pte. Ltd.
2 Woodlands Spectrum, #03-10
Woodlands Sector 1
Singapore 738068

HT1000 User Manual (IN-CL-UD-3 Rev.6) © 2020 Curiox Biosystems Pte. Ltd. For Research Use Only
| 11
Chapter 2:
Functional Description
Chapter Overview
• Introduction
• Functional Description

HT1000 User Manual (IN-CL-UD-3 Rev.6) © 2020 Curiox Biosystems Pte. Ltd. For Research Use Only
LED (
Spill Tray) Tray In
-
Out/Select
Up/ Down
Wash No
Initial Volume
Start/ Pause
Display Panel
12 | Chapter 2: Functional Description
Introduction
This chapter gives a detailed overview of the parts of the HT1000 and the phases which outline a typical
washing process.
Functional Description
The simple digital interface located on the top front panel of the HT1000 allows operators to adjust the
number of desired wash cycles and the initial sample volume which will be shown numerically on the
display. The main components of the HT1000 are shown in Figure 2-1, Figure 2-2 and Figure 2-3.
Figure 2-1: Front view of the Laminar Wash HT1000 with main components labeled.

HT1000 User Manual (IN-CL-UD-3 Rev.6) © 2020 Curiox Biosystems Pte. Ltd. For Research Use Only
Power Jack from
Universal Power
Adapter
On/ Off Switch
Outlet
Inlet
(White port) (Red port)
Filter
Functional Description | 13
Figure 2-2: Lateral (right side) view of the Laminar Wash HT1000 with main components labeled.
Figure 2-3: Back view of the Laminar Wash HT1000 with main components labeled.
Laminar W
ash
Plate
in
Fluidics Chamber
Feeder Loader
Spill Tray

HT1000 User Manual (IN-CL-UD-3 Rev.6) © 2020 Curiox Biosystems Pte. Ltd. For Research Use Only
14 | Chapter 2: Functional Description
Wash Cycle Process
The plate wash cycle process is as follows, a schematic representation of the process is shown in Figure
2-4.
1. Feeder platform detects for presence of plate and retracts into the machine.
2. Fluidics head lowers down into plate.
3. Based on indicated initial volume, dispensing head tops up or removes each well content until
80µl (e.g. if initial volume is 50µl, dispensing head adds 30µl to the droplet).
4. Aspirating head aspirates full volume based on internal firmware. It is intended that contact
between aspirating nozzles and well droplet breaks before aspiration is completed. Once the
aspiration nozzle completes the aspiration of existing drops at a given height of a nozzle, it
continues to aspirate air. Resultant residual volume should be approximately 25µl based on the
height of an aspiration nozzle.
5. Dispensing head tops up well contents based on internal firmware.
6. Steps 4 and 5 are repeated until stipulated number of washes is reached. Note: if only 1 wash is
specified, the machine will only run until step 4.
7. Feeder platform extends out of the machine, returning plates with approximately 25µl residual
volume in each well.
2 nozzles per droplet on Laminar Wash Plate. Cell washing via laminar flow in 2 to 4 mins.
Figure 2-4: Laminar Wash 96-well Plate washing process on the HT1000

HT1000 User Manual (IN-CL-UD-3 Rev.6) © 2020 Curiox Biosystems Pte. Ltd. For Research Use Only
| 15
Chapter 3:
Setup
Chapter Overview
• Unlocking and Installation
• Preparation for Operation

HT1000 User Manual (IN-CL-UD-3 Rev.6) © 2020 Curiox Biosystems Pte. Ltd. For Research Use Only
16 | Chapter 3: Setup
Unlocking and Installation
Upon arrival of the HT1000 package, check that all the accessories listed below are present. Some of
the components of the HT1000 have been secured with brackets to prevent from damage during
transportation. Remove all brackets shown in the steps below before installing and powering up the
HT1000.
List of items:
 Laminar Wash HT1000 Station
 Fluidic Head
 Power Adapter (Output 24V, 3A)
 Electrical Power Cord
 RS232 to USB Cable
 Allen Key Set
 User Manual in USB Thumb Drive
 Declaration of Conformity (for CE
compliant)
 Filter
 1m Tubing, Red Coded Ring on Connector
connected to Filter
 1m Tubing, White Coded Ring on
Connector
 Dummy Plate
 Calibration Plate
 10ml Manual Priming Syringe with 0.1m
Tubing
 Buffer Inlet Bottle Cap
 Nozzle & Plunger Replacement Tools
 Spare Nozzles & Plungers Set (Aspirating
& Dispensing)
 Spare 3.5 ID O-rings (10X Red, 3X Black)
Unlocking
1. Lift machine top cover and push hinge to lock the cover in the open position.
Figure 3-1: Lift the machine top cover and lock in the open position.
Top
Cover

HT1000 User Manual (IN-CL-UD-3 Rev.6) © 2020 Curiox Biosystems Pte. Ltd. For Research Use Only
Unlocking | 17
2. Hold and pull down the two ends of the supporting bracket to remove. Please note that some
force may be required for this step. The supporting bracket must be kept in a secured location for
any transportation which may arise in the future. For convenience, we would suggest taping it to
the back of the machine.
Figure 3-2: Remove the machine supporting bracket.
3. Using a size 3 Allen key, loosen bolts (x3) by turning to the anti-clockwise direction. Do not over
loosen bolts. Move the locking bracket to the right.
Figure 3-7: Loosen bolts to move locking bracket to the right.
Supporting
Bracket
Bolt 1
Bolt 2
Bolt 3
Locking
Bracket