Curiox Laminar Wash MINI 1000 System User manual

Laminar Wash MINI 1000 System (DC-1000-08-01)
MINI1000 User Manual (IN-MI-UD-1 Rev.9) © 2021 Curiox Biosystems For Research Use Only
Laminar Wash™ MINI 1000 System
User Manual
Copyright © 2021 Curiox Biosystems. All rights reserved.
Curiox Biosystems Co. Ltd
Ace Gasan Tower #215
121 Digital-ro
Geumcheon-gu, Seoul, 08505
South Korea
T: [phone]
E: [e-mail]
W: www.curiox.com
Laminar Wash MINI 1000 System User Manual
Rev. 9, March 2021

Laminar Wash MINI 1000 System (DC-1000-08-01)
MINI1000 User Manual (IN-MI-UD-1 Rev.9) © 2021 Curiox Biosystems For Research Use Only
The information contained in this manual is believed to be accurate, but Curiox Biosystems Pte.
Ltd. accepts no liability for any errors it contains, and reserves the right to alter specifications
without notice.
Trademarks
Curiox, the Curiox logo, and Laminar Wash are trademarks or registered trademarks of Curiox
Biosystems. Other marks appearing in these materials are marks of their respective owners.

Laminar Wash MINI 1000 System (DC-1000-08-01)
MINI1000 User Manual (IN-MI-UD-1 Rev.9) © 2021 Curiox Biosystems For Research Use Only
| i
Table of Contents
Chapter 1:
General Information
. . . . . . . . . . . . . . . . . . 1
General Information . . . . . . . . . . . . . . . . . . . . . . 2
Introduction to Laminar Wash MINI 1000. . . . . 2
Technical Specifications . . . . . . . . . . . . . . . . 3
Chemical Compatibility . . . . . . . . . . . . . . . . . . . . 4
Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
User Attention Notifications . . . . . . . . . . . . 5
Chemical Hazards . . . . . . . . . . . . . . . . . . . . . 5
Chemical Waste Hazards . . . . . . . . . . . . . . . 6
Safety Data Sheets . . . . . . . . . . . . . . . . . . . . 6
Instrument Safety Labels. . . . . . . . . . . . . . . . 6
Instrument Safety Features . . . . . . . . . . . . . 7
Safety Precautions . . . . . . . . . . . . . . . . . . . . 8
General Precautions . . . . . . . . . . . . . . . . . . . 8
Prior to System Operation . . . . . . . . . . . . . . 8
CE Mark . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Customer Service and Technical Support . . . . . . 10
Chapter 2:
Functional Description
. . . . . . . . . . . . . . . 11
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Functional Description . . . . . . . . . . . . . . . . . . . . 12
Wash Cycle Process . . . . . . . . . . . . . . . . . . . 13
Chapter 3:
Setup
. . . . . . . . . . . . . . . . . . . . . . . . . . . .. . .14
Unlocking and Installation . . . . . . . . . . . .. . . . .15
Unlocking . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Buffer Inlet Cap Installation . . . . . . . . . . . . 18
Preparation for Operation. . . . . . . . . . . . . . . . . .19
Chapter 4:
Operation
. . . . . . . . . . . . . . . . . . . . . . . . . .20
Before Operating the MINI . . . . . . . . . . . . . . . 21
Operational Safety . . . . . . . . . . . . . . . . . . . . . .21
Power Supply . . . . . . . . . . . . . . . . . . . . . . . . .21
Stop . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21
Machine Homing. . . . . . . . . . . . . . . . . . . . . . 21
Safety Precautions. . . . . . . . . . . . . . . . . . . . . 22
Operational Mode . . . . . . . . . . . . . . . . . . . . . . . . 22
LCD and Buttons. . . . . . . . . . . . . .. . . . . . . . . 22
Wash Cycle . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Service Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Shutdown Procedure . . . . . . . . . . . . . . . . . . .26
Chapter 5:
Troubleshooting and Maintenance
. . . 27
Troubleshooting Checklist . . . . . . . . . . . . . . . . . .28
Technical Support . . . . . . . . . . . . . . . . . . . . . . . . .28
Maintenance Schedule . . . . . . . . . . . . . . . . . . . . .29
Appendix
A:
Machine Operation & Priming Instructions. . . 30
Appendix
B:
Acknowledgement of Decontamination . . . . . . .34
Appendix C
:
Purchase Information and Feedback Form . . . . .36

Laminar Wash MINI 1000 System (DC-1000-08-01)
MINI1000 User Manual (IN-MI-UD-1 Rev.9) © 2021 Curiox Biosystems For Research Use Only
| 1
Chapter 1:
General Information
Chapter Overview
• General Information
• Introduction to the Laminar Wash MINI 1000
• Chemical Compatibility
• Safety
• Customer Service and Technical Support

Laminar Wash MINI 1000 System (DC-1000-08-01)
MINI1000 User Manual (IN-MI-UD-1 Rev.9) © 2021 Curiox Biosystems For Research Use Only
2 | Chapter 1: General Information
General Information
In general, this user manual has been written for the purpose of providing technical, installation,
operating and troubleshooting information to the operators of the Laminar Wash MINI 1000 (MINI). The
content of this manual includes:
• How to set up and operate the MINI
• The principle of operation and modes of function for the MINI
• Safety features of the MINI and precautions to ensure safe operation
• Troubleshooting procedures and maintenance
Introduction to the MINI
The Laminar Wash system enables sample preparation for multi-color flow cytometry, single-cell
‘omics, and other cellular analytical methods. It bypasses the need for centrifugation for washing cells
in suspension. The Laminar Wash technology is designed to maintain samples in a 16-droplet based
format instead of using a conventional microwell with walls. It is a centrifuge-less processing method
using the unique wall-less features of the Laminar Wash technology combined with an automated
washer. The MINI generates a laminar flow for each drop on the Laminar Wash Strip via dual-nozzle
action, with one nozzle dispensing liquid into the drop and the other nozzle aspirating liquid. The
Laminar Wash workflow is superior to the traditional centrifugation method by enabling the
equivalent of multi-centrifuge washes in a one-step automated wash that may be accomplished within
a shorter length of time.