Curiox Laminar wash mini 1000 system User manual

Laminar Wash MINI 1000 System (DC-1000-08-01)

Laminar Wash™ MINI 1000 System

User Manual

Curiox Biosystems Co. Ltd

Ace Gasan Tower #215

121 Digital-ro

Geumcheon-gu, Seoul, 08505

South Korea

T: +82 2 3016 7929

E: sales@curiox.com

W: www.curiox.com

Laminar Wash MINI 1000 System User Manual

Rev. 9, March 2021

Laminar Wash MINI 1000 System (DC-1000-08-01)

The information contained in this manual is believed to be accurate, but Curiox Biosystems Pte.

Ltd. accepts no liability for any errors it contains, and reserves the right to alter specifications

without notice.

Trademarks

Curiox, the Curiox logo, and Laminar Wash are trademarks or registered trademarks of Curiox

Biosystems. Other marks appearing in these materials are marks of their respective owners.

Laminar Wash MINI 1000 System (DC-1000-08-01)

| i

Table of Contents

Chapter 1:

General Information

. . . . . . . . . . . . . . . . . . 1

General Information . . . . . . . . . . . . . . . . . . . . . . 2

Introduction to Laminar Wash MINI 1000. . . . . 2

Technical Specifications . . . . . . . . . . . . . . . . 3

Chemical Compatibility . . . . . . . . . . . . . . . . . . . . 4

Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5

User Attention Notifications . . . . . . . . . . . . 5

Chemical Hazards . . . . . . . . . . . . . . . . . . . . . 5

Chemical Waste Hazards . . . . . . . . . . . . . . . 6

Safety Data Sheets . . . . . . . . . . . . . . . . . . . . 6

Instrument Safety Labels. . . . . . . . . . . . . . . . 6

Instrument Safety Features . . . . . . . . . . . . . 7

Safety Precautions . . . . . . . . . . . . . . . . . . . . 8

General Precautions . . . . . . . . . . . . . . . . . . . 8

Prior to System Operation . . . . . . . . . . . . . . 8

CE Mark . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Customer Service and Technical Support . . . . . . 10

Chapter 2:

Functional Description

. . . . . . . . . . . . . . . 11

Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Functional Description . . . . . . . . . . . . . . . . . . . . 12

Wash Cycle Process . . . . . . . . . . . . . . . . . . . 13

Chapter 3:

Setup

. . . . . . . . . . . . . . . . . . . . . . . . . . . .. . .14

Unlocking and Installation . . . . . . . . . . . .. . . . .15

Unlocking . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Installation . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Buffer Inlet Cap Installation . . . . . . . . . . . . 18

Preparation for Operation. . . . . . . . . . . . . . . . . .19

Chapter 4:

Operation

. . . . . . . . . . . . . . . . . . . . . . . . . .20

Before Operating the MINI . . . . . . . . . . . . . . . 21

Operational Safety . . . . . . . . . . . . . . . . . . . . . .21

Power Supply . . . . . . . . . . . . . . . . . . . . . . . . .21

Stop . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21

Machine Homing. . . . . . . . . . . . . . . . . . . . . . 21

Safety Precautions. . . . . . . . . . . . . . . . . . . . . 22

Operational Mode . . . . . . . . . . . . . . . . . . . . . . . . 22

LCD and Buttons. . . . . . . . . . . . . .. . . . . . . . . 22

Wash Cycle . . . . . . . . . . . . . . . . . . . . . . . . . . 23

Service Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Shutdown Procedure . . . . . . . . . . . . . . . . . . .26

Chapter 5:

Troubleshooting and Maintenance

. . . 27

Troubleshooting Checklist . . . . . . . . . . . . . . . . . .28

Technical Support . . . . . . . . . . . . . . . . . . . . . . . . .28

Maintenance Schedule . . . . . . . . . . . . . . . . . . . . .29

Appendix

A:

Machine Operation & Priming Instructions. . . 30

Appendix

B:

Acknowledgement of Decontamination . . . . . . .34

Appendix C

:

Purchase Information and Feedback Form . . . . .36

Laminar Wash MINI 1000 System (DC-1000-08-01)

| 1

Chapter 1:

General Information

Chapter Overview

• General Information

• Introduction to the Laminar Wash MINI 1000

• Chemical Compatibility

• Safety

• Customer Service and Technical Support

Laminar Wash MINI 1000 System (DC-1000-08-01)

2 | Chapter 1: General Information

General Information

In general, this user manual has been written for the purpose of providing technical, installation,

operating and troubleshooting information to the operators of the Laminar Wash MINI 1000 (MINI). The

content of this manual includes:

• How to set up and operate the MINI

• The principle of operation and modes of function for the MINI

• Safety features of the MINI and precautions to ensure safe operation

• Troubleshooting procedures and maintenance

Introduction to the MINI

The Laminar Wash system enables sample preparation for multi-color flow cytometry, single-cell

‘omics, and other cellular analytical methods. It bypasses the need for centrifugation for washing cells

in suspension. The Laminar Wash technology is designed to maintain samples in a 16-droplet based

format instead of using a conventional microwell with walls. It is a centrifuge-less processing method

using the unique wall-less features of the Laminar Wash technology combined with an automated

washer. The MINI generates a laminar flow for each drop on the Laminar Wash Strip via dual-nozzle

action, with one nozzle dispensing liquid into the drop and the other nozzle aspirating liquid. The

Laminar Wash workflow is superior to the traditional centrifugation method by enabling the

equivalent of multi-centrifuge washes in a one-step automated wash that may be accomplished within

a shorter length of time.

Laminar Wash MINI 1000 System (DC-1000-08-01)

Technical Specifications | 3

Technical Specifications

Description Specification

P

h

y

si

ca

l

Dimensions 232 mm H x 275 mm W x 187.5 mm D

W

eig

h

t

3.5

kg

Elect

r

i

ca

l

V

o

l

ta

g

e

R

equi

r

eme

n

t

1

00

-

2

4

0

V

(+/

-

10%

flu

c

tuation

in nominal voltage

)

Voltage

Input

24V

⎓

3.0A

Environmental

Environmen

t

Indoor Use

Operating Temperature

Ran

g

e

4 - 28 °C

O

pe

r

a

ting

Humidity

<

80%,

non

-

c

o

n

densing

Altitude

Up to 2000m

Pollution Degree

Degree 2

Operation

Plate Type

Laminar Wash

Strip

in

16

-

well

f

orm

a

t

Capacity

1

strip

per operation

Performance

Flow rate at nozzle

5

-

20

µ

L

/s

Volume capacity

80µ

L

per nozzle

at factory settings

Wash sequence

8

wells simultaneous washing

Dilution factor per cycle

Approx.

3.5

times

at factory settings

Bulk flow rate for priming

Approx.

300

m

L

/ min

User Interface

Display

LCD display

Input control

6

input buttons

Number of washes per cycle

50

selectable

washes

External Interface

Electrical inlet

Power inlet jack

1x liquid inlet

A connector for incoming fresh wash buffer

1x liquid outlet

A connector for outgoing waste wash buffer

Laminar Wash MINI 1000 System (DC-1000-08-01)

4 | Chapter 1: General Information

Chemical Compatibility

The components exposed to fluids and reagents are composed of materials which were selected to

be resistant against common chemical substances. However, some common disinfectants should

not be used when decontaminating the MINI. Table 1-1 on page 4 lists the material composition of

the main components of the MINI and shows some reagents which do not pose issues when in

contact with these components. However, some reagents are not compatible with the component

materials, and prolonged contact should be avoided to prevent corrosion and damage.

Component Material Approved

Chemicals

Incompatible

Chemicals

Internal base

structure Stainless Steel (304)

Ethanol, Benzene,

Chloroform, Acetaldehyde,

Propylene Glycol,

Isopropanol, Formaldehyde,

Phenol, Grease, Potassium

Permanganate

Hypochlorite bleach,

Sulfuric Acid

Some minor

components of

main body

Delrin

(Polyoxymethylene)

Ethanol, Benzene, Soap

Solutions, Tetrahydrofuran,

Formaldehyde, Propylene

Glycol, Isopropanol,

Potassium Permanganate

Acetic acid, Grease,

Ketones, Ozone,

Phenol, Ammonia,

Hypochlorite bleach,

Iodine, Hydrogen

Peroxide, Phosphoric

Acid, Sodium Hydroxide

(>50%)

Internal

structural parts

Stainless Steel (304)

Ethanol, Benzene,

Chloroform, Acetaldehyde,

Propylene Glycol,

Isopropanol,

Formaldehyde, Phenol,

Grease, Potassium

Permanganate

Hypochlorite bleach,

Sulfuric Acid

Tubing Plastic Acids, Bases, Alcohols, Salts Ketones, Hydrocarbons

Table 1-1: Chemical compatibility between the component materials in the MINI, including common reagents

and disinfectants. (Adapted from Ingersoll Rand Industrial Technologies, 2008 & K-mac Plastics).

Laminar Wash MINI 1000 System (DC-1000-08-01)

Safety | 5

Safety

User Attention Notifications

Several user attention phrases are used throughout this manual. Each phrase should draw the

following level of attention from the user:

NOTE Points out useful information.

IMPORTANT Indicates information necessary for proper instrument operation.

CAUTION Cautions users regarding potentially hazardous situations with regard to user injury

or damage to the instrument if the information is not heeded.

!WARNING! Warns users that serious physical injury can result if warning precautions are not

heeded.

Chemical Hazards

!WARNING! CHEMICAL HAZARD

Some chemicals used can be potentially hazardous, and can cause injury or illness.

• Read and understand the Safety Data Sheets (SDS) provided by the chemical manufacturer

before you store, handle, or work with any chemicals or hazardous materials.

• Minimize contact with and inhalation of chemicals. Wear appropriate personal

protective equipment when handling chemicals (e.g., safety glasses, gloves, or clothing).

For additional safety guidelines, consult the SDS.

• Do not leave chemical containers open.

• Check regularly for chemical leaks or spills. If a leak or spill occurs, follow the

manufacturer's clean up procedures as recommended on the SDS.

• Comply with all local, state/provincial, or national laws and regulations related to chemical

storage, handling, and disposal.

Laminar Wash MINI 1000 System (DC-1000-08-01)

6 | Chapter 1: General Information

Chemical Waste Hazards

• Read and understand the Safety Data Sheets (SDS) provided by the manufacturers of the

chemicals in the waste container before you store, handle, or dispose of chemical waste.

• Minimize contact with chemical waste. Wear appropriate personal protective equipment

when handling chemicals (e.g., safety glasses, gloves, or clothing).

• Use precaution when emptying the waste bottle.

• Dispose of waste bottle contents in accordance with good laboratory practices and local, state/

provincial, or national environmental and health regulations.

Safety Data Sheets

Some chemicals used with the MINI may be listed as hazardous. Warnings are displayed on the labels

of all chemicals when hazards exist.

SDS provides users with safety information needed to store, handle, transport and dispose of

the chemicals safely. Curiox recommends updating laboratory MSDS records periodically.

Instrument Safety Labels

The following safety labels are located on the MINI. The safety alert symbol indicates a potential

safety hazard.

Symbol Description

Pinch point hazard and sharp needles caution. Access is restricted to

trained maintenance personnel.

Separate collection for electrical and electronic equipment.

Potential Biohazards. Some assays or specimens may pose a biohazard.

Adequate safety precautions should be taken as outlined in the

specimen’s package insert.

Table 1-2: Instrument safety labels

Laminar Wash MINI 1000 System (DC-1000-08-01)

Instrument Safety Features | 7

Instrument Safety Features

Power Supply

The universal power adapter can accommodate electrical power in the range of 100 - 240 V. The

resultant output to the MINI power jack is 24 V DC.

Disconnecting Device

When operating the machine, always position the power adapter at a location that is easily

accessible to the user. Disconnect machine by detaching the power adapter plug.

Stop

Activating the START/ STOP button in the middle of a washing cycle will cause the machine to halt

activity immediately. The machine returns to home position when the START/ STOP button is

pressed again.

Figure 1-3: Stop Button and Rail Guard

Machine Homing

In the event that power to the machine is cut off in the middle of a washing cycle and subsequently

switched on again, the machine will return to home position.

Rail Guard

A rail guard (see Figure 1-3) is installed as a safety feature to prevent user’s access to moving

plunger block during machine operation. Access to plunger block is restricted to trained

maintenance personnel.

START/ STOP

RAIL GUARD

Laminar Wash MINI 1000 System (DC-1000-08-01)

8 | Chapter 1: General Information

Safety Precautions

• At the end of a wash process, remove the strip from the feeder platform immediately

to prevent the wells from drying, stressing cells and/or deteriorating biological

integrity.

• Keep the area around the power supply free from liquid.

• During active operation, keep your hands off the instrument other than using the display panel.

• If there is an unexpected error, reset the instrument by turning the power switch off and

back on.

General Precautions

• Do not use any other strip except the specified 16 wells format Laminar Wash Strip with the

MINI.

• Do not load more than one Laminar Wash Strip in the MINI at a time.

• Use only the supplied power adapter cord for electrical supply to the unit.

• Do not allow particles larger than 200 µm to enter any of the liquid tubes.

• Be careful not to spill liquid onto the interior of the MINI.

• Always perform a cleaning cycle with an appropriate cleaning solution at the end of an

experiment.

• Keep the original packaging material in case the unit should ever need to be shipped.

• Do not attempt to open or remove the instrument casing or motor parts. Doing so will void the

calibration and warranty and may cause permanent damage to the instrument.

• Contact only qualified Curiox personnel for servicing of the MINI.

Prior to System Operation

Ensure that all users of the MINI have:

• Received instruction in general safety practices for laboratories.

• Received instruction in specific safety practices for the instrument.

• Received instruction on handling of biohazards if biohazardous materials are to be used on

the system.

• Read and understood all related SDS.

CAUTION:

Avoid using the MINI in a manner not specified by Curiox. While the system has been

designed to protect the user, this protection may be impaired if the instrument is used

improperly.

Laminar Wash MINI 1000 System (DC-1000-08-01)

CE Mark | 9

CE Mark

Based on the testing described below and information contained herein, this instrument bears

the CE mark.

Directive 2014/30/EU Electromagnetic Compatibility

This device has been type-tested by an independent, accredited testing laboratory and found to

meet the requirements of EN 61326-1:2013/ IEC 61326-1:2012 Electrical equipment for

measurement, control and laboratory use.

Directive 2014/35/EU Low –Voltage Device

This device has been verified and found to meet the requirements of Directive 2014/35/EU

“electrical electronic equipment designed for use within certain voltage limits”.

The device has been type-tested by an independent testing laboratory and was found

to meet the requirements of this Directive. Verification of compliance was conducted to

the limits and methods of the following:

EN 61010-1, “Safety requirement for electrical equipment for measurement, control and

laboratory use. Part 1, General requirements.”

EN 61010-2-081, “Particular requirements for automatic and semi-automatic laboratory

equipment for analysis and other purposes.”

Directive 2015/863 Restriction On the use of Hazardous Substances (ROHS 3)

This device has been verified and found to meet the requirements of Directive 2015/863

“restriction on the use of certain hazardous substances in electrical and electronic equipment”.

Directive 2012/19/EU Waste Electrical and Electronic Equipment (WEEE)

Dispose of the device according to Directive 2012/19/EU, on “waste electrical and electronic

equipment (WEEE)” or local ordinances.

FCC

Based on the testing described below and information contained herein, this instrument bears

the FCC mark.

FCC CFR 47 Part 15B Unintentional Radiators

This device has been type-tested by an independent, accredited testing laboratory and found to

meet the requirements of CFR, Title 47, Part 15B for unintentional radiators.

Laminar Wash MINI 1000 System (DC-1000-08-01)

10 | Chapter 1: General Information

Customer Service and Technical Support

Telephone

+1 650 226 8420 (US)

+65 6908 4201 (Singapore)

Fax

+ 1 650 590 5406 (US)

+65 6908 4203 (Singapore)

E-mail

sales@curiox.com

Web

www.curiox.com

Addresses

Curiox Biosystems, Inc.

400 West Cummings Park

Suite 4350

Woburn, MA 01801

USA

Curiox Biosystems Pte. Ltd.

2 Gambas Crescent

#07-14 Nordcom II (Tower 1)

Singapore 757044

Curiox Biosystems China China Co., Ltd

KR Space #217

No. 328 Hua Shan Road

Jingan, Shanghai 200040

PRC

Curiox Biosystems Co. Ltd

Ace Gasan Tower #215

121 Digital-ro

Geumcheon-gu, Seoul, 08505

South Korea

Laminar Wash MINI 1000 System (DC-1000-08-01)

| 11

Chapter 2:

Functional Description

Chapter Overview

• Introduction

• Functional Description

Laminar Wash MINI 1000 System (DC-1000-08-01)

12 | Chapter 2: Functional Description

Introduction

This chapter gives a detailed overview of the parts of the MINI and the phases which outline a typical

washing process.

Functional Description

The simple interface located on the top front panel of the MINI allows operators to adjust the number

of desired wash cycles and the initial sample volume which will be shown numerically on the display. The

main components of the MINI are shown below.

Figure 2-1: Front and back view of the Laminar Wash MINI with main components labelled.

PRIME-PURGE

PURGE/

ENDLESS

PRIME

-

PURGE

SELECT UP

DOWN

START/

STOP

LCD

display

On/ Off Switch

Outlet Waste Inlet Buffer Power Jack

Machine Front View

Machine Back View

Lever

Feeder

Platform with

Spill Tray

Laminar Wash MINI 1000 System (DC-1000-08-01)

Wash Cycle Process | 13

Wash Cycle Process

The strip wash cycle process is as follows; a schematic representation of the process is shown in Figure

2.2.

1. Load strip onto the feeder platform and select parameters accordingly by using SELECT and UP or

DOWN buttons.

2. Bring the lever down to engage strip and press the START/ STOP button.

3. Dispensing head tops up well content based on the indicated initial volume.

4. Aspirating head aspirates full volume based on internal firmware. It is intended that contact

between aspirating nozzles and well droplet breaks before aspiration is completed. Once the

aspiration nozzles complete the aspiration of existing drops to a residual volume, it continues to

aspirate air.

5. Dispensing head tops up well contents based on internal firmware.

6. Steps 4 and 5 are repeated until the stipulated number of washes are reached, ending with step 4.

NOTE: If only 1 wash is specified, the machine will only run until step 4.

Figure 2-2: Laminar wash on the MINI

Laminar Wash MINI 1000 System (DC-1000-08-01)

14 |

Chapter 3:

Setup

Chapter Overview

• Unlocking and Installation

• Unlocking

• Preparation for Operation

Laminar Wash MINI 1000 System (DC-1000-08-01)

Chapter 3: Setup | 15

Unlocking and Installation

Upon arrival of the MINI package, check that all the accessories listed below are present. Some of the

components of the MINI have been secured with locking screw and pillars to protect against damage

during transportation. Remove locking screw and pillars shown in the steps below before installing

and powering up the MINI.

List of items:

 Laminar Wash MINI 1000

 Power Adapter (Output 24V, 3A)

 Electrical Power Cord

 Allen Key Size 3 for Unlocking

 User Manual in USB Thumb Drive

 Declaration of Conformity

 Filter

 1m Tubing (diameter 3.2mm), Red Coded

Ring on Connector connected to Filter

 1m Tubing (diameter 6.4mm), White

Coded Ring on Connector

 Dummy Strip

 Calibration Slide

 2 Adaptors

 Spill Tray

 Bottle Cap with Inlet and Outlet

Connections (3 pieces)

Unlocking

MINI has been secured by screw and plastic holders to prevent damage to machine moving parts during

transportation. Perform the following steps to unlock the machine.

1. With the use of an Allen Key (size 3), turn anti-clockwise to loosen M4 screw attached to the

machine lever.

Figure 3-1: Unlocking machine lever.

Laminar Wash MINI 1000 System (DC-1000-08-01)

16 | Unlocking

2. Remove plastic holders (2 pieces) that are attached to the machine pillars, one on left front,

and the second on right back.

Figure 3-2: Unlocking machine pillar

3. Perform the same steps in reverse order to prepare machine for transportation.

IMPORTANT: Machine must be properly secured in the manner specified above to prevent damage

to moving parts when transported from one location to another.

Plastic Holders

Laminar Wash MINI 1000 System (DC-1000-08-01)

Chapter 3: Setup | 17

Installation

1. Place the MINI on a flat surface and in an environment with temperature and humidity conditions

as stipulated in “Technical Specifications” on page 3.

2. Connect power cable and delivery tubes to machine in the manner specified below (Figure 3-3).

a. Connect the power cable to the universal power adaptor.

b. Insert the “Power Jack” onto the back of the MINI.

c. Insert the two delivery tubes into the respective ports on the MINI.

 Install a clean filter into one end of the inlet tubing and insert into the “Inlet Buffer” (red

port) on the MINI.

 Insert the other end into wash buffer bottle via the buffer inlet bottle cap. Refer to the

next section “Buffer Inlet Bottle Cap Installation” for installation instructions.

 Insert one end of the outlet tubing into the “Outlet Waste” (white port) on the MINI and the

other end into an empty waste bottle.

IMPORTANT: The buffer inlet bottle cap must not be used on the waste bottle.

Figure 3-3: Connecting power supply as well as inlet and outlet tubing.

Outlet Waste

Inlet Buffer Power Jack

Curiox Laminar Wash MINI 1000 System User manual

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