Curiox Laminar Wash MINI 1000 System User manual

Laminar Wash MINI 1000 System (DC-1000-08-01)
MINI1000 User Manual (IN-MI-UD-1 Rev.9) © 2021 Curiox Biosystems For Research Use Only
Laminar Wash™ MINI 1000 System
User Manual
Copyright © 2021 Curiox Biosystems. All rights reserved.
Curiox Biosystems Co. Ltd
Ace Gasan Tower #215
121 Digital-ro
Geumcheon-gu, Seoul, 08505
South Korea
T: [phone]
E: [e-mail]
W: www.curiox.com
Laminar Wash MINI 1000 System User Manual
Rev. 9, March 2021

Laminar Wash MINI 1000 System (DC-1000-08-01)
MINI1000 User Manual (IN-MI-UD-1 Rev.9) © 2021 Curiox Biosystems For Research Use Only
The information contained in this manual is believed to be accurate, but Curiox Biosystems Pte.
Ltd. accepts no liability for any errors it contains, and reserves the right to alter specifications
without notice.
Trademarks
Curiox, the Curiox logo, and Laminar Wash are trademarks or registered trademarks of Curiox
Biosystems. Other marks appearing in these materials are marks of their respective owners.

Laminar Wash MINI 1000 System (DC-1000-08-01)
MINI1000 User Manual (IN-MI-UD-1 Rev.9) © 2021 Curiox Biosystems For Research Use Only
| i
Table of Contents
Chapter 1:
General Information
. . . . . . . . . . . . . . . . . . 1
General Information . . . . . . . . . . . . . . . . . . . . . . 2
Introduction to Laminar Wash MINI 1000. . . . . 2
Technical Specifications . . . . . . . . . . . . . . . . 3
Chemical Compatibility . . . . . . . . . . . . . . . . . . . . 4
Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
User Attention Notifications . . . . . . . . . . . . 5
Chemical Hazards . . . . . . . . . . . . . . . . . . . . . 5
Chemical Waste Hazards . . . . . . . . . . . . . . . 6
Safety Data Sheets . . . . . . . . . . . . . . . . . . . . 6
Instrument Safety Labels. . . . . . . . . . . . . . . . 6
Instrument Safety Features . . . . . . . . . . . . . 7
Safety Precautions . . . . . . . . . . . . . . . . . . . . 8
General Precautions . . . . . . . . . . . . . . . . . . . 8
Prior to System Operation . . . . . . . . . . . . . . 8
CE Mark . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Customer Service and Technical Support . . . . . . 10
Chapter 2:
Functional Description
. . . . . . . . . . . . . . . 11
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Functional Description . . . . . . . . . . . . . . . . . . . . 12
Wash Cycle Process . . . . . . . . . . . . . . . . . . . 13
Chapter 3:
Setup
. . . . . . . . . . . . . . . . . . . . . . . . . . . .. . .14
Unlocking and Installation . . . . . . . . . . . .. . . . .15
Unlocking . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Buffer Inlet Cap Installation . . . . . . . . . . . . 18
Preparation for Operation. . . . . . . . . . . . . . . . . .19
Chapter 4:
Operation
. . . . . . . . . . . . . . . . . . . . . . . . . .20
Before Operating the MINI . . . . . . . . . . . . . . . 21
Operational Safety . . . . . . . . . . . . . . . . . . . . . .21
Power Supply . . . . . . . . . . . . . . . . . . . . . . . . .21
Stop . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21
Machine Homing. . . . . . . . . . . . . . . . . . . . . . 21
Safety Precautions. . . . . . . . . . . . . . . . . . . . . 22
Operational Mode . . . . . . . . . . . . . . . . . . . . . . . . 22
LCD and Buttons. . . . . . . . . . . . . .. . . . . . . . . 22
Wash Cycle . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Service Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Shutdown Procedure . . . . . . . . . . . . . . . . . . .26
Chapter 5:
Troubleshooting and Maintenance
. . . 27
Troubleshooting Checklist . . . . . . . . . . . . . . . . . .28
Technical Support . . . . . . . . . . . . . . . . . . . . . . . . .28
Maintenance Schedule . . . . . . . . . . . . . . . . . . . . .29
Appendix
A:
Machine Operation & Priming Instructions. . . 30
Appendix
B:
Acknowledgement of Decontamination . . . . . . .34
Appendix C
:
Purchase Information and Feedback Form . . . . .36

Laminar Wash MINI 1000 System (DC-1000-08-01)
MINI1000 User Manual (IN-MI-UD-1 Rev.9) © 2021 Curiox Biosystems For Research Use Only
| 1
Chapter 1:
General Information
Chapter Overview
• General Information
• Introduction to the Laminar Wash MINI 1000
• Chemical Compatibility
• Safety
• Customer Service and Technical Support

Laminar Wash MINI 1000 System (DC-1000-08-01)
MINI1000 User Manual (IN-MI-UD-1 Rev.9) © 2021 Curiox Biosystems For Research Use Only
2 | Chapter 1: General Information
General Information
In general, this user manual has been written for the purpose of providing technical, installation,
operating and troubleshooting information to the operators of the Laminar Wash MINI 1000 (MINI). The
content of this manual includes:
• How to set up and operate the MINI
• The principle of operation and modes of function for the MINI
• Safety features of the MINI and precautions to ensure safe operation
• Troubleshooting procedures and maintenance
Introduction to the MINI
The Laminar Wash system enables sample preparation for multi-color flow cytometry, single-cell
‘omics, and other cellular analytical methods. It bypasses the need for centrifugation for washing cells
in suspension. The Laminar Wash technology is designed to maintain samples in a 16-droplet based
format instead of using a conventional microwell with walls. It is a centrifuge-less processing method
using the unique wall-less features of the Laminar Wash technology combined with an automated
washer. The MINI generates a laminar flow for each drop on the Laminar Wash Strip via dual-nozzle
action, with one nozzle dispensing liquid into the drop and the other nozzle aspirating liquid. The
Laminar Wash workflow is superior to the traditional centrifugation method by enabling the
equivalent of multi-centrifuge washes in a one-step automated wash that may be accomplished within
a shorter length of time.

Laminar Wash MINI 1000 System (DC-1000-08-01)
MINI1000 User Manual (IN-MI-UD-1 Rev.9) © 2021 Curiox Biosystems For Research Use Only
Technical Specifications | 3
Technical Specifications
Description Specification
P
h
y
si
ca
l
Dimensions 232 mm H x 275 mm W x 187.5 mm D
W
eig
h
t
3.5
kg
Elect
r
i
ca
l
V
o
l
ta
g
e
R
equi
r
eme
n
t
1
00
-
2
4
0
V
(+/
-
10%
flu
c
tuation
in nominal voltage
)
Voltage
Input
24V
⎓
3.0A
Environmental
Environmen
t
Indoor Use
Operating Temperature
Ran
g
e
4 - 28 °C
O
pe
r
a
ting
Humidity
<
80%,
non
-
c
o
n
densing
Altitude
Up to 2000m
Pollution Degree
Degree 2
Operation
Plate Type
Laminar Wash
Strip
in
16
-
well
f
orm
a
t
Capacity
1
strip
per operation
Performance
Flow rate at nozzle
5
-
20
µ
L
/s
Volume capacity
80µ
L
per nozzle
at factory settings
Wash sequence
8
wells simultaneous washing
Dilution factor per cycle
Approx.
3.5
times
at factory settings
Bulk flow rate for priming
Approx.
300
m
L
/ min
User Interface
Display
LCD display
Input control
6
input buttons
Number of washes per cycle
50
selectable
washes
External Interface
Electrical inlet
Power inlet jack
1x liquid inlet
A connector for incoming fresh wash buffer
1x liquid outlet
A connector for outgoing waste wash buffer

Laminar Wash MINI 1000 System (DC-1000-08-01)
MINI1000 User Manual (IN-MI-UD-1 Rev.9) © 2021 Curiox Biosystems For Research Use Only
4 | Chapter 1: General Information
Chemical Compatibility
The components exposed to fluids and reagents are composed of materials which were selected to
be resistant against common chemical substances. However, some common disinfectants should
not be used when decontaminating the MINI. Table 1-1 on page 4 lists the material composition of
the main components of the MINI and shows some reagents which do not pose issues when in
contact with these components. However, some reagents are not compatible with the component
materials, and prolonged contact should be avoided to prevent corrosion and damage.
Component Material Approved
Chemicals
Incompatible
Chemicals
Internal base
structure Stainless Steel (304)
Ethanol, Benzene,
Chloroform, Acetaldehyde,
Propylene Glycol,
Isopropanol, Formaldehyde,
Phenol, Grease, Potassium
Permanganate
Hypochlorite bleach,
Sulfuric Acid
Some minor
components of
main body
Delrin
(Polyoxymethylene)
Ethanol, Benzene, Soap
Solutions, Tetrahydrofuran,
Formaldehyde, Propylene
Glycol, Isopropanol,
Potassium Permanganate
Acetic acid, Grease,
Ketones, Ozone,
Phenol, Ammonia,
Hypochlorite bleach,
Iodine, Hydrogen
Peroxide, Phosphoric
Acid, Sodium Hydroxide
(>50%)
Internal
structural parts
Stainless Steel (304)
Ethanol, Benzene,
Chloroform, Acetaldehyde,
Propylene Glycol,
Isopropanol,
Formaldehyde, Phenol,
Grease, Potassium
Permanganate
Hypochlorite bleach,
Sulfuric Acid
Tubing Plastic Acids, Bases, Alcohols, Salts Ketones, Hydrocarbons
Table 1-1: Chemical compatibility between the component materials in the MINI, including common reagents
and disinfectants. (Adapted from Ingersoll Rand Industrial Technologies, 2008 & K-mac Plastics).

Laminar Wash MINI 1000 System (DC-1000-08-01)
MINI1000 User Manual (IN-MI-UD-1 Rev.9) © 2021 Curiox Biosystems For Research Use Only
Safety | 5
Safety
User Attention Notifications
Several user attention phrases are used throughout this manual. Each phrase should draw the
following level of attention from the user:
NOTE Points out useful information.
IMPORTANT Indicates information necessary for proper instrument operation.
CAUTION Cautions users regarding potentially hazardous situations with regard to user injury
or damage to the instrument if the information is not heeded.
!WARNING! Warns users that serious physical injury can result if warning precautions are not
heeded.
Chemical Hazards
!WARNING! CHEMICAL HAZARD
Some chemicals used can be potentially hazardous, and can cause injury or illness.
• Read and understand the Safety Data Sheets (SDS) provided by the chemical manufacturer
before you store, handle, or work with any chemicals or hazardous materials.
• Minimize contact with and inhalation of chemicals. Wear appropriate personal
protective equipment when handling chemicals (e.g., safety glasses, gloves, or clothing).
For additional safety guidelines, consult the SDS.
• Do not leave chemical containers open.
• Check regularly for chemical leaks or spills. If a leak or spill occurs, follow the
manufacturer's clean up procedures as recommended on the SDS.
• Comply with all local, state/provincial, or national laws and regulations related to chemical
storage, handling, and disposal.

Laminar Wash MINI 1000 System (DC-1000-08-01)
MINI1000 User Manual (IN-MI-UD-1 Rev.9) © 2021 Curiox Biosystems For Research Use Only
6 | Chapter 1: General Information
Chemical Waste Hazards
• Read and understand the Safety Data Sheets (SDS) provided by the manufacturers of the
chemicals in the waste container before you store, handle, or dispose of chemical waste.
• Minimize contact with chemical waste. Wear appropriate personal protective equipment
when handling chemicals (e.g., safety glasses, gloves, or clothing).
• Use precaution when emptying the waste bottle.
• Dispose of waste bottle contents in accordance with good laboratory practices and local, state/
provincial, or national environmental and health regulations.
Safety Data Sheets
Some chemicals used with the MINI may be listed as hazardous. Warnings are displayed on the labels
of all chemicals when hazards exist.
SDS provides users with safety information needed to store, handle, transport and dispose of
the chemicals safely. Curiox recommends updating laboratory MSDS records periodically.
Instrument Safety Labels
The following safety labels are located on the MINI. The safety alert symbol indicates a potential
safety hazard.
Symbol Description
Pinch point hazard and sharp needles caution. Access is restricted to
trained maintenance personnel.
Separate collection for electrical and electronic equipment.
Potential Biohazards. Some assays or specimens may pose a biohazard.
Adequate safety precautions should be taken as outlined in the
specimen’s package insert.
Table 1-2: Instrument safety labels

Laminar Wash MINI 1000 System (DC-1000-08-01)
MINI1000 User Manual (IN-MI-UD-1 Rev.9) © 2021 Curiox Biosystems For Research Use Only
Instrument Safety Features | 7
Instrument Safety Features
Power Supply
The universal power adapter can accommodate electrical power in the range of 100 - 240 V. The
resultant output to the MINI power jack is 24 V DC.
Disconnecting Device
When operating the machine, always position the power adapter at a location that is easily
accessible to the user. Disconnect machine by detaching the power adapter plug.
Stop
Activating the START/ STOP button in the middle of a washing cycle will cause the machine to halt
activity immediately. The machine returns to home position when the START/ STOP button is
pressed again.
Figure 1-3: Stop Button and Rail Guard
Machine Homing
In the event that power to the machine is cut off in the middle of a washing cycle and subsequently
switched on again, the machine will return to home position.
Rail Guard
A rail guard (see Figure 1-3) is installed as a safety feature to prevent user’s access to moving
plunger block during machine operation. Access to plunger block is restricted to trained
maintenance personnel.
START/ STOP
RAIL GUARD