Curiox Laminar Wash HT2100 Station 96 User manual

Laminar Wash™ HT2100 Station 96 [DC-2100-96-01]
HT2100 User Manual (IN-CL-UD-06 Rev 02) © 2023 Curiox Biosystems For Research Use Only
Laminar Wash™ HT2100 Station
96 User Manual
Copyright © 2023 Curiox Biosystems. All rights reserved.
Curiox Biosystems Pte. Ltd.
2 Gambas Crescent
#07-14 Nordcom II (Tower 1)
Singapore 757044
T: [phone]
F: [phone]
E: [email protected]
W: www.curiox.com
Laminar Wash HT2100 User Manual
Document Nr: IN-CL-UD-06
Revision 02, September 2023

Laminar Wash™ HT2100 Station 96 [DC-2100-96-01]
HT2100 User Manual (IN-CL-UD-06 Rev 02) © 2023 Curiox Biosystems For Research Use Only
The information contained in this manual is believed to be accurate, but Curiox Biosystems Pte. Ltd.
accepts no liability for any errors it contains and reserves the right to alter specifications without notice.
IMPORTANT NOTICE
Please carefully adhere to all instructions provided in this User Manual, as failure to do so may result in
the invalidation of your service contract.
Trademarks
Curiox, the Curiox logo, and Laminar Wash are trademarks or registered trademarks of Curiox
Biosystems. Other marks appearing in these materials are marks of their respective owners.

Laminar Wash™ HT2100 Station 96 [DC-2100-96-01]
HT2100 User Manual (IN-CL-UD-06 Rev 02) © 2023 Curiox Biosystems For Research Use Only
Table of Contents
Chapter 1:
General Information ............................................................................. 1
General Information ............................................................................ 2
Introduction to the Laminar Wash HT2100 ................................. 2
Technical Specifications ............................................................. 3
Chemical Compatibility ...................................................................... 4
Safety ......................................................................................................... 6
User Attention Notifications ..................................................... 6
Chemical Hazards ....................................................................... 6
Chemical Waste Hazards .......................................................... 7
Material Safety Data Sheets .................................................... 7
Instrument Safety Labels ........................................................... 7
Instrument Safety Features ....................................................... 8
Safety Precautions ....................................................................... 8
General Precautions .................................................................... 9
Prior to System Operation ........................................................ 9
CE Mark .................................................................................................. 10
Customer Service and Technical Support ..................................... 11
Instrument Storage and Shipping ................................................. 12
Chapter 2:
Functional Description ..................................................................... 13
Introduction ........................................................................................... 14
Functional Description ...................................................................... 14
Wash Cycle Process .................................................................. 16
Chapter 3:
Setup ....................................................................................................... 17
Installation ............................................................................................. 18
Installation ................................................................................... 19
Buffer Inlet Bottle Cap Installation ...................................... 24
Interconnection to Accessories ....................................................... 25
PRI & SEC Port Connection .................................................... 25
RS232 Serial Port Connection ............................................... 26
Preparation for Operation ............................................................... 27
Chapter 4:
Operation .............................................................................................. 32
Before Operating the HT2100 .............................................................. 33
Operational Safety ............................................................................... 33
Plate Detection .......................................................................... 33
Power Supply .............................................................................. 33
Emergency Stop ......................................................................... 33
Machine Homing ....................................................................... 33
Washer Locking ......................................................................... 33
Front Protective Panel ............................................................. 33
Spill Tray ...................................................................................... 34
Safety Precautions .................................................................... 34
Operation Mode ................................................................................... 34
User Interface ............................................................................. 34
Function Buttons ....................................................................... 37
Wash Cycle .................................................................................. 38
Service Mode ......................................................................................... 39
User Interface ............................................................................. 39
Function buttons ....................................................................... 39
Settings Mode ....................................................................................... 41
User Interface ............................................................................. 41
Function buttons ....................................................................... 41
Startup Procedure ............................................................................... 42
Shutdown Procedure .......................................................................... 42
Timer ....................................................................................................... 43
Service & Engineering ........................................................................ 43
Washer Operations ............................................................................. 46
Volume Calibration .................................................................. 46
Daily Shutdown Procedure .................................................... 47
Washer Maintenance ......................................................................... 49
Cleaning and Maintenance of Auto-prime Tray ............ 49
Fluidics Decontamination ...................................................... 52
Long Term Dry Storage Procedure ...................................... 56
Preventive Maintenance – Weekly Automated Prime .. 57
Error Codes ............................................................................................ 59
Chapter 5:
Maintenance and Troubleshooting ............................................. 60
Maintenance Schedule ....................................................................... 61

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HT2100 User Manual (IN-CL-UD-06 Rev 01) © 2023 Curiox Biosystems For Research Use Only
Common Problems for Troubleshooting ..................................... 62
Technical Support ................................................................................ 68
Decontamination Procedure ........................................................... 68
Appendix A:
Acknowledgment of Decontamination ..................................... 69
Acknowledgment of Decontamination Form ............................. 70
Appendix B:
Purchase Information and Feedback .......................................... 71
Purchase Information and Feedback Form .................................. 72

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HT2100 User Manual (IN-CL-UD-06 Rev 01) © 2023 Curiox Biosystems For Research Use Only
Chapter 1:
General Information
Chapter Overview
• General Information
• Introduction to the Laminar Wash HT2100
• Chemical Compatibility
• Safety
• CE Mark
• Customer Service and Technical Support
• Instrument Storage and Shipping

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HT2100 User Manual (IN-CL-UD-06 Rev 01) © 2023 Curiox Biosystems For Research Use Only
General Information
In general, this user manual has been written for the purpose of providing technical, installation,
operating and troubleshooting information to the operators of the Laminar Wash™ HT2100 Station. The
content of this manual includes:
• How to set up and operate the HT2100
• The principle of operation and modes of function for the HT2100
• Safety features of the HT2100 and precautions to ensure safe operation.
• Troubleshooting procedures and maintenance
Introduction to the Laminar Wash HT2100
The Laminar Wash solution enables sample preparation for multi-color flow cytometry, single-cell
sequencing, and other cellular analytical methods. It bypasses the need of centrifugation for washing
cells in suspension. The Laminar Wash technology is designed to maintain samples in a 96-droplet based
format instead of using a conventional microwell with walls. It is a centrifuge-less processing method
using the unique wall-less features of the Laminar Wash technology combined with a fully automated
washer. The Laminar Wash HT2100 generates a laminar flow for each drop on the Laminar Wash 96-
well Plate via dual-nozzle action, with one nozzle dispensing liquid into the drop and the other nozzle
aspirating liquid. The Laminar Wash workflow is superior to the traditional centrifugation method.

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Chapter 1: General Information | 3
HT2100 User Manual (IN-CL-UD-06 Rev 02) © 2023 Curiox Biosystems For Research Use Only
Technical Specifications
Description
Specification
Physical
Dimensions
310 mm H x 262 mm W x 302 mm D
Weight
14 kg
Electrical
Voltage Requirement
100 - 240 V
Equipment Ratings
Input Voltage
24.0 V
Input Current
3.0 A
Environmental
Indoor or Outdoor Use
Indoor
Operating Temperature Range
4 - 28 °C
Operating Humidity
< 80%, non-condensing
Operation
Plate Type
Laminar Wash Plate in 96-well format, Single-use.
Capacity
1 plate per operation
Performance
Flow rate at nozzle
5-20µl/s
Volume capacity
80µl per nozzle
Wash sequence
96 wells simultaneous washing
Dilution factor per cycle
Approx. 3.5 times
Bulk flow rate for priming
Approx. 300ml/min
Well Residual Volume
Average
26 ± 3µl
Maximum
< 30µl
Minimum
> 20µl
User Interface
Display
7-inch Raspberry Pi Display
Input control
Touch screen interface
Number of washes per cycle
1x to 50x selectable
External Interface
1x fluid inlet connector (red)
A connector for incoming fresh wash buffer
1x fluid outlet connector (white)
A connector for outgoing waste wash buffer
1x RS232
A plug-in for updating operating parameters

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HT2100 User Manual (IN-CL-UD-06 Rev 01) © 2023 Curiox Biosystems For Research Use Only
Chemical Compatibility
The components exposed to fluids and reagents are composed of materials which were selected to be
resistant against common chemical substances. However, some common disinfectants should not be
used when decontaminating the HT2100. Table 1-1 on page 4 lists the material composition of the main
components of the HT2100 and shows some reagents which do not pose issues when in contact with
these components. However, some reagents are not compatible with the component materials, and
prolonged contact should be avoided to prevent corrosion and damage.
Table 1-1: Chemical compatibility between the component materials and accessories in HT2100, including common
reagents and disinfectants. (Adapted from: CP LabSafety www.calpaclab.com, USPlastic
www.usplastic.com/catalog/files/charts/Tygon%20CC.pdf accessed Jan 2023)
Component
Material
Approved Chemicals
Incompatible Chemicals
Internal base
structure
Stainless Steel (304)
Ethanol, Benzene,
Chloroform, Acetaldehyde,
Propylene Glycol,
Isopropanol, Formaldehyde,
Phenol, Grease, Potassium
Permanganate, Hydrogen
Peroxide
Hypochlorite bleach,
Sulfuric Acid
Some minor
components of
main body
Delrin
(Polyoxymethylene)
Ethanol, Benzene, Soap
Solutions, THF,
Formaldehyde, Propylene
Glycol, Isopropanol,
Potassium Permanganate
Acetic acid, Grease,
Ketones, Ozone, Phenol,
Ammonia, Hypochlorite
bleach, Iodine,
Hydrogen Peroxide,
Phosphoric Acid,
Sodium Hydroxide
(>50%)
Bottle cap
Polypropylene
Ethanol, isopropyl alcohol,
detergents, ethylene glycol,
propylene glycol,
formaldehyde (<40%),
dimethyl formamide, sodium
hypochlorite, hydrogen
peroxide, ozone, sulfuric acid
(<75%)
Chloroform
Tubing (bottle
cap, parts of
washer internal
structure)
Silicone
Ethanol, isopropyl alcohol,
detergents, ethylene glycol,
propylene glycol,
formaldehyde, formamide,
sodium hypochlorite,
hydrogen peroxide, sulfuric
acid (<3M), ozone
Chloroform

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HT2100 User Manual (IN-CL-UD-06 Rev 02) © 2023 Curiox Biosystems For Research Use Only
Component
Material
Approved Chemicals
Incompatible Chemicals
Tubing (parts of
washer internal
structure)
Tygon E-3603
Alcoholsa, detergents,
ethylene glycol, propylene
glycol, sodium hypochlorite,
hydrogen peroxide (≤30%),
ozone, sulfuric acid (≤30%)
Ketones, hydrocarbons,
formaldehyde (37%),
dimethyl formamide,
chloroform
Internal and
external Luer
lock
connectors
Nylonb
Chloroform, ethanol,
detergents, ethylene glycol,
propylene glycol,
formaldehyde (<40%),
dimethyl formamide
Isopropyl alcohol,
sodium hypochlorite
(<20%), Hydrogen
peroxide (>10%), ozone,
sulfuric acid (>10%)
Internal
structural parts
Aluminum
Ethanol, Benzene,
Propylene Glycol,
Isopropanol,
Formaldehyde, Ozone,
Grease, Phenol, Hydrogen
Peroxide
Hypochlorite Bleach,
Soap Solutions, Sulfuric
Acid, Potassium
Permanganate,
Phosphoric Acid
External
structure
a Chemical compatibility of Tygon with alcohols is rated fair.
b Nylon is not autoclave-safe. Do not autoclave the assembled bottle caps.

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HT2100 User Manual (IN-CL-UD-06 Rev 01) © 2023 Curiox Biosystems For Research Use Only
Safety
User Attention Notifications
Several user attention phrases are used throughout this manual. Each phrase should draw the following
level of attention from the user:
NOTE Points out useful information.
IMPORTANT Indicates information necessary for proper instrument operation.
CAUTION Cautions the users regarding potentially hazardous situations that could potentially
result in user injury or damage to the instrument if the information is not heeded.
!WARNING! Warn users that serious physical injury can result if warning precautions are not
heeded.
Chemical Hazards
!WARNING! CHEMICAL HAZARD
Some chemicals used can be potentially hazardous and can cause injury or illness.
• Read and understand the Material Safety Data Sheets (MSDSs) provided by the chemical
manufacturer before you store, handle, or work with any chemicals or hazardous materials.
• Minimize contact with and inhalation of chemicals. Wear appropriate personal protective equipment
when handling chemicals (e.g., safety glasses, gloves, or clothing). For additional safety guidelines
consult the MSDS.
• Do not leave chemical containers open.
• Check regularly for chemical leaks or spills. If a leak or spill occurs, follow the manufacturer's cleanup
procedures as recommended on the MSDS.
• Comply with all local, state/provincial, or national laws and regulations related to chemical storage,
handling, and disposal.

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HT2100 User Manual (IN-CL-UD-06 Rev 02) © 2023 Curiox Biosystems For Research Use Only
Chemical Waste Hazards
• Read and understand the Material Safety Data Sheets (MSDS) provided by the manufacturers of the
chemicals in the waste container before you store, handle, or dispose of chemical waste.
• Minimize contact with chemical waste. Wear appropriate personal protective equipment when
handling chemicals (e.g., safety glasses, gloves, or clothing).
• Use precaution when emptying the waste bottle.
• Dispose of waste bottle contents in accordance with good laboratory practices and local,
state/provincial, or national environmental and health regulations.
Material Safety Data Sheets
Some chemicals used with the HT2100 may be listed as hazardous. Warnings are displayed on the labels
of all chemicals when hazards exist.
MSDS provide users with safety information needed to store, handle, transport and dispose of the
chemicals safely. Curiox recommends updating laboratory MSDS records periodically.
Material Safety Data Sheets for Curiox reagents are available upon request by calling 650 226-8420 in
the US or [phone] outside the US. Otherwise call the chemical manufacturer directly or visit their
web site.
Instrument Safety Labels
The following safety label is located on the HT2100. The label displays a safety alert symbol indicating a
potential safety hazard.
Symbol
Description
Pinch point hazard. Keep hands clear.
Separate collection for electrical and electronic equipment.
Potential Biohazards. Some assays or specimens may pose a biohazard.
Adequate safety precautions should be taken as outlined in the specimen’s
package insert.

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HT2100 User Manual (IN-CL-UD-06 Rev 01) © 2023 Curiox Biosystems For Research Use Only
Instrument Safety Features
Plate Detection
The HT2100 detects the presence of a Laminar Wash 96-well Plate. The machine will not begin operation
if a plate is not detected on the feeder platform.
Disconnecting Device
When operating the machine, always position the power adapter at a location that is easily accessible
to the user. Disconnect machine by detaching the power adapter plug.
Power Supply
The universal power adapter accepts electrical power in the voltage range of 100 - 240 V. The resultant
output on the HT2100 power jack is 24 V DC.
Emergency Stop
Activating the emergency stop button in the middle of a washing cycle will cause the machine to
immediately halt operations. The machine goes to home position when the emergency stop button is
subsequently deactivated.
Machine Homing
In the event power to the machine is cut off in the middle of a washing cycle and subsequently switched
on again, the machine will go to home position.
Spill Tray
The spill tray sensor detects excess liquid in the tray to prevent it from spilling onto the machine’s
interior. When this happens, the error code “E31” is prompted on the machine display panel. User may
only access the draining button during this time.
Safety Precautions
• At the end of a wash process, remove the plate from the feeder platform immediately to prevent the
wells from drying, stressing cells and/or deteriorating biological integrity.
• Keep the area around the power supply free from liquid.
• During active operation, keep your hands off the instrument other than using the display panel.
• If there is an unexpected error, reset the instrument by turning the power switch off and back on.

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HT2100 User Manual (IN-CL-UD-06 Rev 02) © 2023 Curiox Biosystems For Research Use Only
General Precautions
• Do not use any other plate except the specified Laminar Wash 96-well Plate with the HT2100.
• Do not load more than one Laminar Wash 96-well Plate into the HT2100 at a time.
• Use only the supplied power adapter cord for electrical supply to the unit.
• Do not allow particles larger than 70 µm to enter any of the liquid tubes. Ensure that the inlet filter
is assembled at the inlet port.
• Be careful not to spill liquid onto the interior of the HT2100.
• Always perform a cleaning cycle with an appropriate cleaning solution at the end of an experiment.
• Keep the original packaging material in case the unit should ever need to be shipped.
• Do not attempt to open or remove the instrument casing or motor parts. Doing so will void the
calibration and warranty and may cause permanent damage to the instrument.
• Contact only qualified Curiox personnel for servicing of the HT2100.
Prior to System Operation
Ensure that all users of the HT2100 have:
• Received instruction in general safety practices for laboratories.
• Received instruction in specific safety practices for the instrument.
• Received instruction on handling of biohazards if biohazardous materials are to be used on the
system.
• Read and understood all related MSDS.
CAUTION
Avoid using the HT2100 in a manner not specified by Curiox. While the system has been
designed to protect the user, this protection may be impaired if the instrument is used
improperly.

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HT2100 User Manual (IN-CL-UD-06 Rev 01) © 2023 Curiox Biosystems For Research Use Only
CE Mark
Based on the testing described below and information contained herein, this instrument bears the CE
mark.
Directive 2014/30/EU Electromagnetic Compatibility
This device has been type-tested by an independent, accredited testing laboratory and found to meet
the requirements of EN 61326-1 for Emissions and Immunity.
Verification of compliance was conducted to the limits and methods of the following:
1. EN 61326-1: 2013 Emissions
a. Harmonics Current Emission (Class A)
b. Voltage Fluctuation/ Flicker
c. Conducted Emission (Group 1 Class B)
d. Radiated Emission (Electric Field) (Group 1 Class B)
2. EN 61326-1: 2013 Immunity
a. Electrostatic Discharge Immunity
b. RF Radiated Immunity
c. Electrical Fast Transient/ Burst Immunity
d. Voltage Surge Immunity
e. Conducted Disturbance Immunity
f. Voltage Dips & Interruptions Immunity
Directive 2014/35/EU Low –Voltage Device
This device has been verified and found to meet the requirements of Directive 2014/35/EU “electrical
electronic equipment designed for use within certain voltage limits”.
Directive 2011/65/EU Restriction On the use of Hazardous Substances (ROHS 2)
This device has been verified and found to meet the requirements of Directive 2011/65/EU “restriction
on the use of certain hazardous substances in electrical and electronic equipment”.
Directive 2012/19/EU Waste Electrical and Electronic Equipment (WEEE)
Dispose of the device according to Directive 2012/19/EU, on “waste electrical and electronic equipment
(WEEE)” or local ordinances.

Laminar Wash™ HT2100 Station 96 [DC-2100-96-01]
Chapter 1: General Information | 11
HT2100 User Manual (IN-CL-UD-06 Rev 02) © 2023 Curiox Biosystems For Research Use Only
Customer Service and Technical Support
Telephone
[phone] (US)
[phone] (Singapore)
Fax
+ 1 650 590 5406 (US)
[phone] (Singapore)
E-mail
[e-mail]
Web
www.curiox.com
Addresses
Curiox Biosystems, Inc.
400 West Cummings Park
Suite 6650
Woburn, MA 01801
USA
Curiox Biosystems Pte. Ltd.
2 Gambas Crescent
#07-14 Nordcom II (Tower 1)
Singapore 757044
Curiox Biosystems China Co., Ltd
Room 201-09, 2nd Floor Building 6
No. 690 Bibo Road, Pudong District,
Shanghai, 201203
China
Curiox Biosystems Co. Ltd
Samsung Harrington IT Tower
#202, #204-213
9 Gil 41 Digital-ro
Geumcheon-gu, Seoul 08511
South Korea

Laminar Wash™ HT2100 Station 96 [DC-2100-96-01]
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HT2100 User Manual (IN-CL-UD-06 Rev 01) © 2023 Curiox Biosystems For Research Use Only
Instrument Storage and Shipping
1. Ensure that all internal and external surfaces of the HT2100 washer station and BEX are dry
following the decontamination procedure above. See Chapter 4, Section Long Term Dry Storage
Procedure for decontamination procedure prior to extended storage of the instrument.
2. Stow the instruments in a regulated storage environment: Temperature: 4-28ºC, Humidity <80%
RH, non-condensing.
3. If it is intended to ship the instruments, seal the machine in an air-tight bag, pack it in its original
packaging prior to transportation.

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HT2100 User Manual (IN-CL-UD-06 Rev 01) © 2023 Curiox Biosystems For Research Use Only
Chapter 2:
Functional Description
Chapter Overview
• Introduction
• Functional Description

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HT2100 User Manual (IN-CL-UD-06 Rev 01) © 2023 Curiox Biosystems For Research Use Only
Introduction
This chapter gives a detailed overview of the parts of the HT2100 and the phases which outline a typical
washing process.
Functional Description
The simple digital interface located on the top front panel of the HT2100 allows operators to adjust the
number of desired wash cycles and the initial sample volume which will be shown numerically on the
display. The main components of the HT2100 are shown in Figure 2-1, Figure 2-2 and Figure 2-3.
Figure 2-1: Front view of the Laminar Wash HT2100

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HT2100 User Manual (IN-CL-UD-06 Rev 02) © 2023 Curiox Biosystems For Research Use Only
Figure 2-2: Lateral (right side) view of the Laminar Wash HT2100
Figure 2-3: Back view of the Laminar Wash HT2100 with main components labelled.
Power Jack from
Universal Power
Adapter
Outlet
(White port)
Inlet
(Red port)
On/ Off Switch
Filter

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HT2100 User Manual (IN-CL-UD-06 Rev 01) © 2023 Curiox Biosystems For Research Use Only
Wash Cycle Process
The plate wash cycle process is as follows; a schematic representation of the process is shown in Figure
2-4.
1. Feeder platform detects for presence of plate and retracts into the machine.
2. Fluidics head lowers down into plate.
3. Based on indicated initial volume, dispensing head tops up or removes each well content until 80
± 2µl (e.g., if initial volume is 50µl, dispensing head adds 30µl to the droplet).
4. Aspirating head aspirates full volume based on internal firmware. It is intended that contact
between aspirating nozzles and well droplet breaks before aspiration is completed. Once the
aspiration nozzle completes the aspiration of existing drops at a given height of a nozzle, it
continues to aspirate air. Resultant average residual volume should be approximately 26 ± 3µl
based on the height of an aspiration nozzle.
5. Dispensing head tops up well contents based on internal firmware.
6. Steps 4 and 5 are repeated until stipulated number of washes is reached. Note: if only 1 wash is
specified, the machine will only run until step 4.
7. Feeder platform extends out of the machine, returning plates with approximately 26 ± 3µl average
residual volume in each well.
2 nozzles per droplet on Laminar Wash Plate. Cell washing via laminar flow in 2 to 4 mins.
Figure 2-4: Laminar Wash 96-well Plate washing process on the HT2100.