Curiox Laminar Wash HT2000 User manual

Laminar Wash™ HT2000 Station 96 [DC-2000-96-01]
HT2000 User Manual (IN-CL-UD-4 Rev.9) © 2022 Curiox Biosystems For Research Use Only
Laminar Wash™HT2000 Station
96 User Manual
Copyright © 2022 Curiox Biosystems. All rights reserved.
Curiox Biosystems Pte. Ltd.
2 Gambas Crescent
#07-14 Nordcom II (Tower 1)
Singapore 757044
T: [phone]
F: [phone]
E: [e-mail]
W: www.curiox.com
LaminarWashHT2000 User Manual
Revision 9, June 2022

Laminar Wash™ HT2000 Station 96 [DC-2000-96-01]
HT2000 User Manual (IN-CL-UD-4 Rev.9) © 2022 Curiox Biosystems For Research Use Only
The information contained in this manual is believed to be accurate, but Curiox Biosystems Pte.
Ltd. accepts no liability for any errors it contains and reserves the right to alter specifications
without notice.
Trademarks
Curiox, the Curiox logo, and Laminar Wash are trademarks or registered trademarks of Curiox
Biosystems. Other marks appearing in these materials are marks of their respective owners

Laminar Wash™ HT2000 Station 96 [DC-2000-96-01]
HT2000 User Manual (IN-CL-UD-4 Rev.9) © 2022 Curiox Biosystems For Research Use Only
Table of Contents
Chapter 1:
General Information . . . . . . . . . . . . . . . . . . 1
General Information . . . . . . . . . . . . . . . . . . . . . . 2
Introduction to the Laminar Wash HT2000 ..... 2
Technical Specifications . . . . . . . . . . . . . . . . 3
Chemical Compatibility . . . . . . . . . . . . . . . . . . . .4
Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
User Attention Notifications . . . . . . . . . . . . 5
Chemical Hazards . . . . . . . . . . . . . . . . . . . . . 5
Chemical Waste Hazards . . . . . . . . . . . . . . . 6
Material Safety Data Sheets . . . . . . . . . . . . 6
Instrument Safety Labels. . . . . . . . . . . . . . . . 6
Instrument Safety Features . . . . . . . . . . . . . 7
Safety Precautions . . . . . . . . . . . . . . . . . . . .7
General Precautions . . . . . . . . . . . . . . . . . . . 8
Prior to System Operation . . . . . . . . . . . . . . 8
CE Mark . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Customer Service and Technical Support . . . . . 10
Chapter 2:
Functional Description . . . . . . . . . . . . . . . 11
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Functional Description . . . . . . . . . . . . . . . . . . . .12
Wash Cycle Process. . . . . . . . . . . . . . . . . . .14
Chapter 3:
Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15
Installation . . . . . . . . . . . . . . . . .. . . . . . . . . . . . 15
Installation . . . . . . . . . . . . . . . . . . . . . . . . . .16
Buffer Inlet Bottle Cap Installation. . . . . . .23
Interconnection to Accessories . . . . . . . . . . . . .24
PRI & SEC Port Connection . . . . . . . . . . . . .24
RS232 Serial Port Connection. . . . . . . . . . . 24
Preparation for Operation . . . . . . . . . . . . . . . . 25
Chapter 4:
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Before Operating the HT2000. . . . . . . . . . . . . 29
Operational Safety . . . . . . . . . . . . . . . . . . . . . . 29
Plate Detection . . . . . . . . . . . . . . . . . . . . . . 29
Power Supply . . . . . . . . . . . . . . . . . . . . . . . . 29
Emergency Stop. . . . . . . . . . . . . . . . . . . . . . 29
Machine Homing. . . . . . . . . . . . . . . . . . . . . 29
Spill Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Safety Precautions. . . . . . . . . . . . . . . . . . . . 30
Operational Mode . . . . . . . . . . . . . . . . . . . . . . . 30
User Interface . . . . . . . . . . . . . . . . . . . . . . . . 30
Function Buttons. . . . . . . . . . . . . . . . . . . . . . . 31
Wash Cycle . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Service Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
User Interface . . .. . . . . . . . . . . . . . . . . . . . . . 32
Function Tab . . . . . . . . . . . . . . . . . . . . . . . . . .32
Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Shutdown Procedure. . . . . . . . . . . . . . . . . . . 35
Error Codes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Chapter 5:
Maintenance and Troubleshooting . . . 37
Maintenance Schedule. . . . . . . . . . . . . . . . . . .38
Common Problems for Troubleshooting. . . . . .39
Technical Support . . . . . . . . . . . . . . . . . . . . . . . .45
Decontamination Procedure . . . . . . . . . . . . . . 45
Appendix A:
Acknowledgement of Decontamination. . . . . 46
Acknowledgement of Decontamination Form . .47
Appendix B:
Purchase Information and Feedback . . . . . . .48
Purchase Information and Feedback Form. . . . .49
Addendum :
Preventive Maintenance Once a Week . . . . .50
Start-Up/Shutdown Procedure. . . . . . . . . . . . . . 50

Laminar Wash™ HT2000 Station 96 [DC-2000-96-01]
HT2000 User Manual (IN-CL-UD-4 Rev.9) © 2022 Curiox Biosystems For Research Use Only
| 1
Chapter 1:
General Information
Chapter Overview
• General Information
• Introduction to the LaminarWashHT2000
• Chemical Compatibility
• Safety
• CE Mark
• Customer Service and Technical Support

Laminar Wash™ HT2000 Station 96 [DC-2000-96-01]
HT2000 User Manual (IN-CL-UD-4 Rev.9) © 2022 Curiox Biosystems For Research Use Only
2 | Chapter 1: General Information
General Information
In general, this user manual has been written for the purpose of providing technical, installation,
operating and troubleshooting information to the operators of the LaminarWash™HT2000 Station. The
content of this manual includes:
•How to set up and operate the HT2000
•The principle of operation and modes of function for the HT2000
•Safety features of the HT2000 and precautions to ensure safe operation
•Troubleshooting procedures and maintenance
Introduction to the Laminar Wash HT2000
The Laminar Wash solution enables sample preparation for multi-color flow cytometry, single-cell
sequencing, and other cellular analytical methods. It bypasses the need of centrifugation for washing
cells in suspension. The Laminar Wash technology is designed to maintain samples in a 96-droplet
based format instead of using a conventional microwell with walls. It is a centrifuge-less processing
method using the unique wall-less features of the Laminar Wash technology combined with a fully
automated washer. The Laminar Wash HT2000 generates a laminar flow for each drop on the Laminar
Wash 96-well Plate via dual-nozzle action, with one nozzle dispensing liquid into the drop and the
other nozzle aspirating liquid. The Laminar Wash workflow is superior over the traditional
centrifugation method.

Laminar Wash™ HT2000 Station 96 [DC-2000-96-01]
HT2000 User Manual (IN-CL-UD-4 Rev.9) © 2022 Curiox Biosystems For Research Use Only
Chapter 1: General Information | 3
Technical Specifications
Description
Specification
Physical
Dimensions
310 mm H x 262 mm W x 302 mm D
Weight
14 kg
Electrical
Voltage Requirement
100 - 240 V
EquipmentRatings
Input Voltage
24.0 V
Input Current
3.0 A
Environmental
Indoor or Outdoor Use
Indoor
Operating Temperature
Range
4 - 28 °C
Operating Humidity
< 80%, non-condensing
Operation
Plate Type
LaminarWashPlate in 96-well format, Single-use.
Capacity
1 plate per operation
Performance
Flow rate at nozzle
5-20µl/s
Volume capacity
80µl per nozzle
Wash sequence
96 wells simultaneous washing
Dilution factor per cycle
Approx. 3.5 times
Bulk flow rate for
priming
Approx. 300ml/ min
Well Residual Volume
Average
26 ± 3µl
Maximum
< 30µl
Minimum
> 20µl
User Interface
Display
7-inch Raspberry Pi Display
Input control
Touch screen interface
Number of washes per
cycle
1x to 99x selectable
External Interface
1x inlet
A connector for incoming fresh wash buffer
1x outlet
A connector for outgoing waste wash buffer
1x RS232
A plug-in for updating operating parameters

Laminar Wash™ HT2000 Station 96 [DC-2000-96-01]
HT2000 User Manual (IN-CL-UD-4 Rev.9) © 2022 Curiox Biosystems For Research Use Only
4 | Chapter 1: General Information
Chemical Compatibility
The components exposed to fluids and reagents are composed of materials which were selected to
be resistant against common chemical substances. However, some common disinfectants should
not be used when decontaminating the HT2000. Table 1-1 on page 4 lists the material composition
of the main components of the HT2000 and shows some reagents which do not pose issues when
in contact with these components. However, some reagents are not compatible with the
component materials, and prolonged contact should be avoided to prevent corrosion and damage.
Component
Material
Approved
Chemicals
Incompatible
Chemicals
Internal base
structure
Stainless Steel (304)
Ethanol, Benzene,
Chloroform, Acetaldehyde,
Propylene Glycol,
Isopropanol, Formaldehyde,
Phenol, Grease, Potassium
Permanganate, Hydrogen
Peroxide
Hypochlorite bleach,
Sulfuric Acid
Some minor
components of
main body
Delrin
(Polyoxymethylene)
Ethanol, Benzene, Soap
Solutions, THF,
Formaldehyde, Propylene
Glycol, Isopropanol,
Potassium Permanganate
Acetic acid, Grease,
Ketones, Ozone,
Phenol, Ammonia,
Hypochlorite bleach,
Iodine, Hydrogen
Peroxide, Phosphoric
Acid, Sodium Hydroxide
(>50%)
Internal
structural parts
Aluminium
Ethanol, Benzene,
Propylene Glycol,
Isopropanol,
Formaldehyde, Ozone,
Grease, Phenol, Hydrogen
Peroxide
Hypochlorite Bleach,
Soap Solutions, Sulfuric
Acid
,
Potassium
Permanganate,
Phosphoric Acid
External
structure
Table 1-1: Chemical compatibility between the component materials in the HT2000, including common
reagents and disinfectants. (Adapted from Ingersoll Rand Industrial Technologies, 2008 & K-mac Plastics).

Laminar Wash™ HT2000 Station 96 [DC-2000-96-01]
HT2000 User Manual (IN-CL-UD-4 Rev.9) © 2022 Curiox Biosystems For Research Use Only
Chapter 1: General Information | 5
Safety
User Attention Notifications
Several user attention phrases are used throughout this manual. Each phrase should draw the
following level of attention from the user:
NOTE Points out useful information.
IMPORTANT Indicates information necessary for proper instrument operation.
CAUTION Cautions users regarding potentially hazardous situations in regard to user
injury or damage to the instrument if the information is not heeded.
!WARNING! Warn users that serious physical injury can result if warning precautions are
not heeded.
Chemical Hazards
!WARNING! CHEMICAL HAZARD
Some chemicals used can be potentially hazardous and can cause injury or illness.
•Read and understand the Material Safety Data Sheets (MSDSs) provided by the chemical
manufacturer before you store, handle, or work with any chemicals or hazardous
materials.
•Minimize contact with and inhalation of chemicals. Wear appropriate personal
protective equipment when handling chemicals (e.g., safety glasses, gloves, or clothing).
For additional safety guidelines consult the MSDS.
•Do not leave chemical containers open.
•Check regularly for chemical leaks or spills. If a leak or spill occurs, follow the
manufacturer's cleanup procedures as recommended on the MSDS.
•Comply with all local, state/provincial, or national laws and regulations related to chemical
storage, handling, and disposal.

Laminar Wash™ HT2000 Station 96 [DC-2000-96-01]
HT2000 User Manual (IN-CL-UD-4 Rev.9) © 2022 Curiox Biosystems For Research Use Only
6 | Chapter 1: General Information
Chemical Waste Hazards
•Read and understand the Material Safety Data Sheets (MSDSs) provided by the
manufacturers of the chemicals in the waste container before you store, handle, or dispose
of chemical waste.
•Minimize contact with chemical waste. Wear appropriate personal protective equipment
when handling chemicals (e.g., safety glasses, gloves, or clothing).
•Use precaution when emptying the waste bottle.
•Dispose of waste bottle contents in accordance with good laboratory practices and local, state/
provincial, or national environmental and health regulations.
Material Safety Data Sheets
Some chemicals used with the HT2000 may be listed as hazardous. Warnings are displayed on the
labels of all chemicals when hazards exist.
MSDSs provide users with safety information needed to store, handle, transport and dispose of
the chemicals safely. Curiox recommends updating laboratory MSDS records periodically.
Material Safety Data Sheets for Curiox reagents are available upon request by calling 650 226-8420
in the US or [phone] outside the US. Otherwise call the chemical manufacturer directly or
visit their web site.
Instrument Safety Labels
The following safety label is located on the HT2000. The label displays a safety alert symbol indicating
a potential safety hazard.
Symbol
Description
Pinch point hazard. Keep hands clear.
Separate collection for electrical and electronic equipment.
Potential Biohazards. Some assays or specimens may pose a biohazard.
Adequate safety precautions should be taken as outlined in the specimen’s
package insert.

Laminar Wash™ HT2000 Station 96 [DC-2000-96-01]
HT2000 User Manual (IN-CL-UD-4 Rev.9) © 2022 Curiox Biosystems For Research Use Only
Chapter 1: General Information | 7
Instrument Safety Features
Plate Detection
The HT2000 detects the presence of a Laminar Wash 96-well Plate. The machine will not begin
operation if a plate is not detected on the feeder platform.
Disconnecting Device
When operating the machine, always position the power adapter at a location that is easily
accessible to the user. Disconnect machine by detaching the power adapter plug.
Power Supply
The universal power adapter is able to accommodate electrical power in the range of 100 - 240 V.
The resultant output to the HT2000 power jack is 24 V DC.
Emergency Stop
Activating the emergency stop button in the middle of a washing cycle will cause the machine to
immediately freeze. The machine goes to home position when the emergency stop button is
subsequently deactivated.
Machine Homing
In the event power to the machine is cut off in the middle of a washing cycle and subsequently
switched on again, the machine will go to home position.
Spill Tray
The spill tray sensor detects excess liquid in the tray to prevent it from spilling onto the machine’s
interior. When this happens, the error code “E31” is prompted on the machine display panel. User
may only access the draining button during this time.
Safety Precautions
•At the end of a wash process, remove the plate from the feeder platform immediately
to prevent the wells from drying, stressing cells and/or deteriorating biological
integrity.
•Keep the area around the power supply free from liquid.
•During active operation, keep your hands off the instrument other than using the display panel.
•If there is an unexpected error, reset the instrument by turning the power switch off and
back on.