Step 1 : Culture cells
Step 2 : Distribute cell suspension to multi well plates
Step 3 : Cell aggregate formation
PrimeSurface MS-9096UZ (S-bio) plates are critical as the
machine stage and suction pressure are optimized for them
Distribute 100 µL/ well cell suspension to cell culture well
plates:
•5-50 thousands of cells / well*
•Spheroids of single or multiple cell types can be used
Culture for about 48 h in a CO2incubator (37℃, 5% CO2)
Less incubation time may cause loosely formed spheroids
difficult to use for 3D printing.
Check diameters of aggregates with a microscope, they should
be 450-600 μm for optimal 3D printing.
Culture enough cells for your 3D printing project.
It is recommended to use cells in/near a log growth phase.
6
Cyfuse Biomedical K.K
ICN Building-5A 2-27-17, Hongo, Bunkyo-ku, Tokyo, 113-0033, JAPAN
TEL：+81-3-4455-7872 E-mail：sales@cyfusebm.com URL：http://www.cyfusebio.com
Prior to 3D Printing 2:
Making Spheroids (Cell Aggregates )
For optimal 3D printing, spheroids must be in good
condition. Please determine the best protocol for your
cells in advance.
Concentration
(cells/mL)
400,000 200,000 100,000 50,000
Cells / well
(cells/uL)
40,000 20,000 10,000 5,000
Diameter
after 48h
600um 500um 360um 280um
*Seeding condition depends on cell type, please perform a preliminary test
with your cells to find out the best conditions as following this example:
●This Manual is subject to change without notice. ●Information is as of Feb, 2016.
