diagenode Bioruptor Pico User manual

Bioruptor® Pico
Elegant, Precise, Advanced
Your best shearing device

Dear Customer,
Thank you for choosing our device and for your confidence in Diagenode.
We have designed this device using our expertise in shearing so that it exceeds your expectations. This device
will serve your research needs for many years and will provide the best experience with sample preparation.

INTENDED USE
The Bioruptor Pico is intended for laboratory use. It is not suitable for clinical use. Use of the Bioruptor Pico in any
manner other than as directed herein could cause harm to persons and may void the warranty. Diagenode will not be
responsible for injury or damage resulting from improper use of the Bioruptor Pico.
WARNING
Carefully read the following instructions before using your Bioruptor to avoid the risk of personal injury and damage.
• Do not use the Bioruptor Pico for anything other than its intended use.
• After removing the packaging, make sure that the Bioruptor Pico is intact.
• Do not use the Bioruptor Pico in a cold room.
• Do not turn the instrument to the ON position without water.
• Use deionized or distilled water.
• Do not tilt or jar the sonication unit.
• Do not exceed 30 minutes of total sonication (total ON cycle).
• Do not allow the Bioruptor Pico to be used by untrained individuals.
• Do not use any accessories not recommended by Diagenode.
• Do not use the Bioruptor Pico if it is showing any sign of breakdown or malfunction.
• Do not attempt to dismantle or repair.
• Check the ‘Good practices’ section in the ‘Settings’ of your Bioruptor.

DESCRIPTION
TECHNICAL SPECIFICATIONS
Input Voltage Range 100 – 240 V
Input Frequency Range 50-60 Hz
Maximum Electrical
Consumption 4.2 A
Ultrasonic Wave Frequency 20 – 60 kHz
Unit Dimensions
• The sonicator
• The cooler
380 (W) x 315 (D) x 275 (H) mm
200 (W) x 390 (D) x 495 (H) mm
Weight
• The sonicator
• The cooler
9 kg
26.9 kg
Placement Indoor areas
Ambient Temperature Range 15 – 25°C
Maximum Relative
Humidity
80%
Altitude Up to 2,000 meters
Degree of protection IP31
Shearing Accessories • Tube holder for 0.2 ml tubes
Cat. No. B01201144
• Tube holder for 0.65 ml tubes
Cat. No. B01201143
• Tube holder for 1.5 ml tubes
Cat. No. B01201140
• 15 ml sonication accessories
Cat. No. B01200016

Shearing Consumables • 0.2 ml Bioruptor Pico Microtubes
Cat. No. C30010020
• 0.65 ml Bioruptor Pico Microtubes
Cat. No. C30010011
• 1.5 ml Bioruptor Pico Microtubes
Cat. No. C30010016
• 15 ml Bioruptor Pico Tubes
Cat. No. C30010017
• 15 ml Bioruptor Pico Tubes &
sonication beads
Cat. No. C01020031
Throughput • 0.2 ml microtubes – 16 samples
• 0.65 ml microtubes – 12 samples
• 1.5 ml microtubes – 6 samples
• 15 ml tubes – 6 samples
Sample Volume Range • 0.2 ml microtubes – 20-100 µl
• 0.65 ml microtubes – 100 µl
• 1.5 ml microtubes – 100-300 µl
• 15 ml tubes – 500 µl – 2 ml
Fragment Length
Range Achievable 150 – 1,000 bp
Working Temperature
Range
2 - 20°C
Variable Parameters Time ON, Time OFF,
# cycles, level of frequency
User Interface Touchscreen with Bioruptor Pico
software

Power cord
Sonication water bath
Touch screen
Motorized lid
Tube holder
Valve connection cable for Bioruptor® Cooler
Tubing
BIORUPTOR® PICO
BIORUPTOR® COOLER
Dock
Led light bar
Speaker
Lid
DESCRIPTION

LED LIGHT BAR
The LED light bar allows the tracking of the processing of your samples.
The blue light means the system is waiting for the operator.
The progressive green light indicates the progression of the shearing process.
The green light indicates the end of the shearing process.
The orange light means the protocol is on pause.
The red light indicates an alert message.

INTERFACE
DESCRIPTION
User protocols: Record your protocol
Guidelines: Read Diagenode
recommendations for your applications
Go & Shear: Start a new shearing
protocol
Good Practices: Critical steps for Bioruptor
maintenance and efficient shearing
Settings: Visualize and change the
parameters of your Bioruptor Pico
Sound: Adjust the sound level
Language: Choose your voice
language
Brightness: Adjust the brightness of
the screen

Technical Support: Diagenode
contact information for technical
questions
Diagenode dots: Go back
to the top menu
Commercial Support: Diagenode
contact information for
commercial questions
Information: General information
about your Bioruptor Pico
Edit: Edit the parameter
Question mark: Some notes to guide you
Maintenance: Access for
administrators

1 2
INSTRUCTIONS
If not, fill the sonication water bath with
~ 700 ml of deionized or distilled water
and the cooling system with 2-3 liters of
deionized or distilled water.
Start the sonicator
Check if water is in the sonication
water bath and the cooler
Maximum volume
Minimum volume
Level indicator on the cooler

Start the cooling system, set the temperature to
4°C and let the water cool down
Start a new shearing experiment
3 4
A. Press ENTER button to reach set point menu
B. Select a set point temperature (4°C) using the
arrow buttons
C. Press ENTER button to apply the temperature
PROTOCOLS
Or find your pre-recorded shearing
protocol on User protocols

Select parameters (Time ON, Time OFF, #
cycles, type of tube or level of frequency)*
Find validated parameters on the Easy Mode
or optimize your protocol with different levels
of frequency on the Advanced Mode.
5 6
INSTRUCTIONS
Select your mode
*Visit our Guidelines folder to get
recommendations for your application.
GO AND SHEAR
Choose validated
protocols
Choose your level
of frequency
GO AND SHEAR (easy mode)
30
seconds
seconds
cycles
30
10
10
min seconds
00

7Fill the sonication tubes
with same volume of
sample*
8
*Use the recommended tubes and sample
volumes for optimal shearing efficiency. Visit our
Guidelines folder for more information.
Vortex and then spin your samples

Fill all positions of the tube holder
with your samples* and place it on the
motorized lid
9Close the lid 10
INSTRUCTIONS
*or with water of same volume

Start the protocol
11 Let your Bioruptor run 12
GO AND SHEAR (easy mode)
30
seconds
seconds
cycles
30
10
10
min seconds
00

Collect your samples
13

If you have any questions, please contact a specialist at
www.diagenode.com/en/pages/support

EQUIPMENT CARE
The water bath must be filled with deionized or distilled water. Change water at least once every month and
clean the sonication tank with a soft tissue. BUT be careful to not turn ON the instrument without water.
MANUAL
SAFETY APPROVAL
Bioruptor® has been assessed and certified under the standards.
ENVIRONMENTAL PROTECTION
Packaging materials contain recyclable materials.
Read the full manual
“
https://www.diagenode.com/en/documents/bioruptor2-manual ” - Tab Documents

WARRANTY
Diagenode guarantees the Bioruptor Pico from any manufacturing defects as we rigorously test it to meet strict
quality standards. Diagenode warrants that all standard components of the instrument will be free of defects
in materials and workmanship for a period of five (5) years or for maximum 210 hours of total running time*
from the date that the warranty period begins, unless the original quotation or accompanying documentation
states a different warranty period. The warranty period begins on the date of delivery and apply only to the first
purchaser of the product. The warranty period expires when one of the two above conditions ends (5 years
or 210 hours of total running time*). If a manufacturing defect arises and a valid claim is received within the
warranty period, Diagenode, at its discretion, will repair or replace the product in accordance with the warranty
terms and conditions stated herein. In case of repair or replacement of a product under warranty, Diagenode
will cover the expenses to return the repaired or replacement product.
This warranty covers only manufacturing defects and does not cover any damage caused by misuse, lack of
compliance to recommendations stated in the manual, neglect, accidents, abrasion, or exposure to extreme
temperatures, chemical solvents, or acids. Improper or incorrectly performed maintenance or repairs will void
the warranty.
*The total running time corresponds to total sonication time ON + total sonication time OFF that compose the
cycles of the shearing process.

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