Hardy Diagnostics QuickSlide AFB-PRO Mounting instructions

Rev. 052316dc AFB-PRO User Manual Page 2 of 8
Table of Contents
Introduction..........................................................................................3
1.0 Intended Use....................................................................................................... 4
2.0 Operator Responsibility –Safety Instructions....................................... 4
2.1 Explanation of Safety Notes .......................................................................... 4
2.2 Safety Instructions ......................................................................................... 4
3.0 Instrument Setup.............................................................................................. 5
4.0 Operating Instructions.................................................................................... 5
4.1 General Guidelines......................................................................................... 5
4.2 Stain Procedure.............................................................................................. 6
4.3 Reagent Replacement..................................................................................... 6
4.4 Menu Options................................................................................................. 7
4.5 Setting Fill Levels.......................................................................................... 7
5.0 Maintenance....................................................................................................... 7
6.0 Service and Supplies........................................................................................ 8
7.0 Safety Data Sheets ............................................................................................ 8

Rev. 052316dc AFB-PRO User Manual Page 3 of 8
Congratulations!
You have made an excellent choice for your Lab. Hardy Diagnostics thanks you for the
trust you have placed in our products and services.
This operating manual has been designed to help you gain an understanding of the
operation and application of your AFB-PRO. For optimal utilization of all functions, we
recommend that you thoroughly study this manual prior to beginning operation.
This manual has been prepared as an aid for all operations and maintenance, which
can be carried out in your facility.
The QuickSlide™ Quality Management System
Our devices for the medical laboratory are developed, produced, and distributed
according to the requirements of ISO 9001:2008. All products are shipped to you from
our manufacturing facility located at 3411 McNiel Ave., Suite 302, Wichita Falls, Texas
76308
Unpacking and Inspecting
Carefully unpack the AFB-PRO and accessories. Check for damage incurred during
transit. Keep all packing material until you are sure the unit operates properly. Any
damage to the shipping box should be reported to the responsible carrier or postal
authority. These instructions must be followed for us to guarantee our full support of
your claim for protecting against loss from concealed damage. The form required for
filing such a claim will be provided by the carrier.
Printed in U.S.A. Changes without prior notification reserved.
Important: Keep this operating manual for future use.
© 2016 All Rights Reserved
QuickSlide™™, a Division of Hardy Diagnostics
3411 McNiel, #302
Wichita Falls, TX 76308
800-295-9588
www.HardyDiagnostics.com
[e-mail]

Rev. 052316dc AFB-PRO User Manual Page 4 of 8
1.0 Intended Use
The AFB-PRO microbiology stainer has been designed to automate most procedures that
use a Modified Auramine O Stain and Modified Auramine O Decolorizer Staining
process for Acid Fast Bacillus samples.
2.0 Operator Responsibility –Safety Instructions
The AFB-PRO ensures safe operation when installed, operated, and maintained according
to common safety regulations. This section explains all the potential dangers that may
arise when operating the AFB-PRO and also specifies the most important safety
precautions to prevent mishaps.
The operator is responsible for the qualification of the personnel operating the
instruments. The operator and personnel is also advised to refer to this Operating Manual
and/or call the QuickSlide™ Technical Support phone number for additional assistance.
2.1 Explanation of Safety Notes
2.2 Safety Instructions
CAUTION:
Describes a possibly dangerous situation. If this is not avoided, slight or
minor injuries could result. A warning of possible property damage may
also be contained in the text.
CAUTION:
Instrument must to be placed on a level operating surface.
Read and understand all instructions and safety precautions listed
in this manual before installing and operating the unit.
Only use the required QuickSlide™ AFB-PRO staining reagents
(Cat. no. AFB-0070).
Only use the required QuickSlide™tubing kits (Cat. no. AFB-
0020).
Always make sure tubing is placed correctly and is not leaking.
Always turn off the unit and disconnect the power supply before
performing any service or maintenance procedures or moving the
unit.

Rev. 052316dc AFB-PRO User Manual Page 5 of 8
3.0 Instrument Setup
3.1 Initial Start-Up
3.1.1 Before plugging the wall transformer into an AC outlet, connect the round
barrel receptacle to the instrument. The Connector is located on the left-
hand side of the instrument directly under the on/off switch.
3.1.2 Turn the power switch off and plug the wall transformer into the unit.
3.1.3 Place the Reagent Pack on the shelf behind the instrument and connect the
Reagent Pack phone type connector to the receptacle on the right-hand
side of the instrument. If the Reagent Pack is not plugged in when the unit
is turned on, the message "SELF-TEST FAILURE: Pack not connected"
will appear. Turn unit off, connect Reagent Pack and turn unit on to
continue.
3.1.4 Remove the caps from the Reagent Pack and insert the properly labeled
reagent cannulas in the appropriate bottles. Customer needs to supply DI
water container and place DI labeled line in the water for the rinse cycles.
3.1.5 Place the waste tube in the waste container.
3.1.6 Turn the instrument on. The instrument will go through a self-test and then
display “prime” or “continue”
3.1.7 Press <F1> to prime. Two primes are required on initial set up.
4.0 Operating Instructions
4.1 General Guidelines
4.1.1 Make sure the specimen smears are thoroughly dry before placing them
into the cuvette.
4.1.2 Use high quality clean slides. Slides that have variable thickness may
have variable staining results.
CAUTION:
The AFB-PRO will only accept stain packs with an included module sold by
Hardy Diagnostics’ QuickSlide™ to ensure optimal results. The instrument is
calibrated only for these reagents. The use of other stain packs or solutions will
void the warranty.
Always remove the tubing kit cannulas from bottles before
moving unit
Transport unit with care
Dropping the unit may cause internal and external damage to the
instrument
Never operate the unit with damaged power supply
Repairs are to be carried out only by qualified service personnel

Rev. 052316dc AFB-PRO User Manual Page 6 of 8
4.1.3 Replace the tubing every 6 to 12 months following the maintenance
protocol.
4.1.4 Do not leave the stained slide in the cuvette for an extended period of
time. It will not dry as rapidly and quality could diminish.
4.2 Stain Procedure
4.2.1 After priming the unit, press <F4> to continue.
4.2.2 The cuvette will be prepared.
4.2.3 Load the slide and press <STAIN>.
4.2.4 When done, remove slide and press <F4> to continue.
4.2.5 Wipe the back side of the slide and let the sample air dry or place within
two sheets of bibulous paper. After the sample has dried, it is ready to be
viewed under the microscope.
4.3 Reagent Replacement
4.3.1 Turn the instrument off.
4.3.2 Remove the cannulas from the empty Reagent Pack.
4.3.3 Unplug the phone connector from the instrument and remove the empty
Reagent Pack. Don’t forget to empty the waste container at this time.
4.3.4 Place the new Reagent Pack on the instrument, connect the NEW phone
connector from the NEW Reagent Pack, and place the reagent cannulas
in the new reagent bottles and insure DI water line is in a source for DI
water.
4.3.5 Turn the instrument on and the Self-Test menu will be displayed. The
cuvette is automatically drained and the Main Menu is displayed.
4.3.6 It is best to prime the unit twice before staining.
CAUTION:
When loading and unloading the slides, be sure you pull it all of the way
out STRAIGHT up. Otherwise, the slides can break inside the slide holder
resulting in unit malfunction. It will also produce sharp objects that could
injure the operator.
CAUTION:
When changing reagent kits, it is important to regularly check the waste
bottle. It is used repeatedly and requires being emptied. If not emptied, it
could result in an overflow of reagent that could potentially lead to damage
to the unit’s surroundings.

Rev. 052316dc AFB-PRO User Manual Page 7 of 8
4.4 Menu Options
The MENU key allows you to customize your stain and decolorizer times.
QuickSlide™recommends the factory settings of one minute in each staining
solution. The rinse time is automatic and is not adjustable.
4.5 Setting Fill Levels
The fill levels are factory set for a standard 1”x3” slide. However, the fill levels
can be custom set to the operator's preference.
4.1.1 Unit must be primed before setting fill levels.
4.1.2 Turn unit off and then turn back on and wait for the main menu. Once the
main menu is displayed, turn the unit off again.
4.1.3 Select <F4>.
4.1.4 Load a blank slide in the cuvette.
4.1.5 Press <F4> until desired fill level is achieved. All three reagents are set
simultaneously.
4.1.6 Press <F1> to drain the cuvette. Once the cuvette is drained, press the
menu key to save the new fill level settings.
5.0Maintenance
The orange pump tubes and color coded lines must be replaced every 6 to 12 months
depending on use.
To perform this procedure, do the following:
5.1 Remove the lines from the stain bottles.
5.2 Prime the unit twice to remove fluid from the old tubes.
5.3 Shut the unit off.
5.4 Unscrew the two thumbscrews holding the pump cover in place.
5.5 Remove the lines one at a time and replace starting from left to right. The longest
lines are to be positioned on the left side of the orange pump tubes and the shorter
lines should be on the right side of the pump tubes. The longer lines are to go into
the reagents bottles and the shorter lines will go to the slide holder. The proper
fittings are labeled with numbers under the slide holder for the shorter lines
coming off of the right side of each orange pump tube.
Pump #1 furthest to the left is the rinse (white labeled line).
Pump #2 is the decolorizer (blue labeled line).
Pump #3 is the waste (orange labeled line)
CAUTION:
Fill levels not set with a blank 1x3inch slide inserted in the slide chamber
will be incorrect and lead to overflow of reagents onto operating area. This
can lead to possible damage of the unit’s surroundings.

Rev. 052316dc AFB-PRO User Manual Page 8 of 8
Pump #4 is the stain (yellow labeled line)
5.6 After installation, two primes are required to fill all the reagent and DI water
lines.
6.0 Service and Supplies
If a problem is encountered that is beyond the scope of this manual or additional
assistance is required, contact our QuickSlide™ Technical Support team at
[phone].
If additional supplies are needed, reagents and tubing kits can be ordered through Hardy
Diagnostics’ Customer Service. Call [phone], or go to
www.HardyDiagnostics.com. Alternatively, you can contact your preferred distributor.
Catalog Number
Description
AFB-0070
AFB-PRO Stain Kit
AFB-0020
AFB-PRO Replacement Tubing Kit
AFB-0800
AFB QC Slides (Box of 50)
PF72P
ProSlide, Frosted End, Premium Microscope Slide, 1”x3”,
72/box
7.0 Safety Data Sheets
Safety Data Sheets (SDS) can be found at www.hardydiagnostics.com/sds_search.html