Diamond SureFlow User manual

diamond SureFlow,.

1. Safety Recommendations
Please read the instruction manual carefully before using the dispenser. Although we have
tried to cover all safety requirements, it is impossible for this manual to prevent all possible
safety hazards. It is the responsibility of the user to establish their own safety requirements and
to determine the necessary precautions prior to using the dispenser.
Please read and follow the following safety recommendations:
•Fully read and adhere to all recommendations and instructions in this manual
•Use protective clothing, eye protection and gloves when working with hazardous liquids.
•Do not dispense inflammable reagents into plastic vessels that can potentially generate a
static charge.
•This device is not suitable for use with the following:
Concentrated {hydrochloric acid, fluorinated hydrocarbons, saline solutions, nitric
acid), highly concentrated alkaline solutions and crystallizing solutions.
Incompatible liquids that attack FEP, PFA, PTFE, borosilicate glass, AbO3, organic
solvents, trifluoroacetic acid, explosive liquids, fuming acids, tetrahydrofuran, suspension {e.g.
charcoal) as solid particles.
•Handle the dispenser carefully to avoid any accidents or spills during usage.
•The nozzle should always point away from the user while dispensing. Avoid splashes.
•Always use suitable vessels for dispensing liquids.
•Never push down on the piston while the nozzle cap is on.
•Clean the discharge tube regularly.
•Do not use excess pressure on the piston while aspirating or dispensing or the glass tube
may break inside. Follow the troubleshooting guide if you experience any difficulty moving
the piston up and down.
•Only use Globe Scientific accessories.
2. Operating Conditions
•+ 15
°
C to +40
°
C {59
°
F to 104
°
F) for bottle top dispenser and reagent
•Vapor pressure upto 500 mbar.
•Densityupto2.2g/cm
3
•Kinematic viscosity upto 500 mm
2
/second
{dynamic viscosity [mPas]=Kinematic Viscosity [mm
2
/s] x density [g/cm
3] )
3. Package Contents
Description
-Dispenser
-Telescopic filling tube
-Recirculation tube*
- Bottle adapters** {Adapter thread sizes : 28, 38, 40 & 45mm)
-Calibration Tool
-Service tool
-Connector {only for 100 ml) {Thread size: 40 / 32 mm)
-User manual
-Certificate of Conformity including calibration certificate
-Warranty card
*This accessory is included in recirculating models only
** 32mm thread size is the standard thread built into each dispenser. No adapter is required for this
thread type
1

4. Overview
,L
®------1] 11
----=--,
@r---CD
1. Cap
2. Head
3. Main Body
4. Pointer
5. Volume Knob
6. Cylinder Sleeve
7. Cylinder
8. Piston
9. Main Valve
10. Valve lever (piston)
11. Recirculation Valve
12. Spout
13. Front Cover
14. Nozzle
15. Nozzle Cap
16. Air Plug
17. Adapter
18. Telescopic Filling Tube
19. Recirculation tube
20. lnnervalve
21. Fillingvalve
�• Note : Recirculation items are only included in recirculating dispenser models.
2

5. Assembly
1. Mounting the telescopic fill
i
ng tube/
recirculation tube
Adjust length of the telescop
i
ng filling tube to the bottle
height and attach it careful
l
y to the cente
r
of the dispenser.
If a dispense
r
w
i
th recirculation is used, the additional
recirculation tube should also be
i
nsta ll ed. Install this tube
w
i
th the cu
r
ved portion po
i
nti ng outward / towards the
nozzle {Fig.1)
2. Mounting the dispenser on a bottle
Carefully screw the dispenser assembly {GL 32 threads)
onto the reagent bottle and ensure that the nozzle is
aligned with the graduation marks on the main body of the
bottle.
@-Note : For bottles with different thread sizes, please select
the appropriate adapter.
The adapters supplied with the dispenser are made of
polypropylene (PP), and can only be used with reagents
which do not attack PP.
WARNING
Always wear protective gloves when touching the
dispenser or the bottle, especially when using dangerous
liquids.
When mounted to a reagent bottle, always carry the
dispenser vertically as shown in Fig. 3
3
Fig. 2
";}
',
}
I{.;
-r::
er:,
5CC
,.
'"
-- >
Fig. 3

6. Priming
6.1 Bottle Top Dispenser with recirculation valve mechanism
A_ WARNING
The nozzle should always point away from the user. Please ensure that the piston is never pressed
down when the nozzle cap is mounted to the dispenser tube. Avoid splashing.
Please follow the steps below to prime the dispenser:
1. Turn the lever to recirculation mode. (Fig. S)
2. Slowly pull up the piston and push it down smoothly to the end stop. Repeat this until
air bubbles disappear from the cylinder. (Fig. 6)
3. Turn the lever back to it's original position for dispensing.
Fig.5
Adjusting recirculation lever
6.2 Bottle Top Dispenser without recirculation valve mechanism
A_ WARNING
The nozzle should always point away from the user. Please ensure that the piston is never pressed
down when the nozzle cap is mounted to the dispenser tube. Avoid splashing.
Please follow the steps below to prime the dispenser:
1. Place a vessel below the nozzle.
2. Hold the spout and carefully remove the
nozzle cap. (Fig. 7)
3. Slowly pull up the piston and push it down
smoothly to the end stop. Repeat this until air
bubbles disappear from the cylinder. (Fig. 6)
4

7. Operating Instructions
7.1 Setting the volume
1) Turn the volume setting knob counterclockwise to unlock.
2) Slide the knob up or down to the desired volume.
3) Align the pointer on the volume setting knob to the desired volume and carefully turn
the knob clockwise to lock at that setting.
Your dispenser is now ready to work at your desired volume. (Fig. 8)
7 .2 Dispensing
&WARNING
mL
Please use caution when dispensing dangerous reagents.
Fig. 8
1. Please follow the priming process (Section 6) before dispensing.
2. Ensure recirculation lever is aligned with nozzle.
3. Make certain a vessel is below the nozzle at all times while dispensing.
4. Set your desired volume (as shown in section 7.1- Setting the Volume).
5. Gently pull the piston up until reaching the upper end stop, then push the
piston down smoothly until reaching the lower end stop.
6. When completed, gently touch the end of nozzle against the inner wall of the
receiving vessel (for touch-off). Repeat this process for each of the dispensing
cycles required.
7. Reattach the nozzle cap to the nozzle when finished.
• Note : Do not use excessive force while pushing down the piston. If you feel the piston is jammed,
•please follow the troubleshooting guide (Section 12)
Please ensure that the piston is at lower stop before reattaching the nozzle cap.
The user may detect a slight sound during aspiration when moving the dispenser; please note this is
normal (due to the ball design used in the piston/ valve).
5

8. Cleaning
Please make sure to clean the dispenser regularly to ensure good operation.
Cleaning should be performed if any of the following occur:
•If the piston becomes stuck inside the tube
•Before changing reagents
•Long periods of downtime
•Before autoclaving
•Before any maintenance
• Please make sure to wear suitable eye protection glasses and protective clothing.
Please follow the steps below prior to cleaning:
1. Completely dispense the liquid present inside the dispenser.
2. Mount the dispenser on a bottle filled with deionized water or any other suitable
cleaning agent and rinse the dispenser several times.
3. Pull off the telescopic tube (and recirculation tube if applicable) and clean with
deionized water.
4. For dispensers with recirculation, set the unit to recirculation mode and again rinse the
dispenser several times.
Please follow the steps below to clean the piston and glass barrel:
• This procedure must be followed if the piston is difficult to move or if dispenser is to be autoclaved.
1. Unscrew the head as shown in Fig. 9
2. Take out the piston completely as shown in Fig.10
3. Clean the piston & glass barrel with deionized water and then reassemble in reverse.
Fig. 9
6
•r -,c, Fig. 10
_I

Disassembly of the valve
1. Put out the Re-circulation tube & telescopic tube (Fig. 11)
2. Use the service tool to unscrew the filling valve and also take out the ball with care. (Fig.12)
3. Use the other side of the service tool to unscrew the inner valve (Fig. 13) and remove it
(Fig.14)
4. After cleaning/replacement, insert inner valve in the same position as before removal.
5. Screw the filling valve first by hand and then tighten it with the service tool.
• If some elastic resistance is present while
aspirating the reagent, then it is possible that
the ball valve may be sticking/stuck.
To correct this, exercise the ball valve using
very light pressure, for example, 200 µI
plastic pipette tip (Fig. 14)
7

9. Accuracy
Error limits related to the nominal capacity {maximum volume) indicated on the instrument are
obtained when instrument and distilled water are equilibrated at ambient temperature
{20 °C/68°F). The calibration process is performed as per the EN ISO 8655-5 standard with a
completely assembled dispenser and uniform dispensing.
Volume Increment Accuracy(±) % Precision (±) %
(ml) (ml) ±% ±ml ±% ±ml
0.25 -2.5 0.05 0.6 0.015 0.2 0.005
0.5 - 5 0.1 0.5 0.025 0.2 0.01
1-10 0.2 0.5 0.05 0.2 0.02
2.5 -25 0.5 0.5 0.125 0.2 0.05
2.5 -30 0.5 0.5 0.15 0.2 0.06
5 - 50 1.0 0.5 0.25 0.2 0.1
5 - 60 1.0 0.5 0.3 0.2 0.12
10 -100 2.0 0.5 0.5 0.2 0.2
10. Calibration
WARNING
During calibration you must begin at the lowest volume first, ensuring the body of the dispenser is not
moved before you close the cap and then proceed to the medium and high volumes.
Calibration check
1. Please follow the calibration procedures as described in EN ISO 8655-5 (i.e. environmental
conditions, water, balance, etc.).
8

Volume adjustment
Please follow these steps to calibrate the dispenser only if there is a variation between the
set volume and actual volume:
1. Open the cap with calibration tool as shown in Fig. 16
2. Insert the hex tool on the hex nut as shown in Fig. 17
3. Rotate the hex tool clockwise to decrease the volume or rotate counterclockwise to
increase the volume.
4. Remove the hex tool and replace the cap.
mL
1
11. Autoclaving
This dispenser can be autoclaved under the following conditions:
Temperature:
Pressure:
Time:
.b).WARNING
121 ·c / 250 ° Fahrenheit
1.05 bar/ 15 psi (pounds)
20 minutes or less
Autoclaving outside the above conditions will damage the dispenser and void the warranty.
Please follow these steps to autoclave the dispenser:
1. Clean the dispenser thoroughly as described in cleaning section before autoclaving.
2. Remove the following parts :
a) the piston, by unscrewing the head
b) the nozzle cap and telescopic filling tube
3. Autoclave all the parts as per the above conditions.
4. Allow the parts to cool at least 8 hours at room temperature and then reassemble.
5. Now the dispenser is ready to be used.
• It is recommended to perform a quick calibration check after autoclaving.
9

12. Troubleshooting
Troubleshooting
Issue Possible root cause Corrective action
Piston moves Dirt disposits or Immediately stop dispensing. Loosen piston
with difficulty with circular motion, but do not disassemble.
or is stuck formation of crystals Follow all cleaning instructions (page 6)
Volume adjusted to Set to required volume {see page 5)
Liquid filling/ minimum setting
dispensing Unscrew the filling valve from the main valve,
problems Filling valve stuck clean it, replace the filling valve if necessary. If
the valve ball is stuck use a 200 µI pipette tip to
loosen it {see page 5)
Pull out the inner valve from the Main valve,
Dispensing Discharge valve stuck clean it, replace the discharge valve if
not possible necessary {see page 5), use a 200 µI plastic
tip to loosen any ball valve that is stuck
Reagent with high vapor
pressure has been drawn Slowly draw in reagent
in too quickly
Valve screw connections Tighten the valves firmly with the service tool
loose
Air bubbles The dispenser has not
in the been primed Prime the dispenser {see page 4)
dispenser Push the filling tube on firmly. If necessary cut
Filling tube is loose or off approx. 1 cm of tube at the upper end and
damaged re-connect it or replace filling tube
Valves not firmly After cleaning the dispenser (page 6),
connected or damaged tighten the valves using the service tool
After cleaning the dispenser {page 6), push the
Filling tube is loose filling tube on firmly. If necessary, cut off approx.
Dispensed or damaged 1 cm of the tube at the upper end and re-connect
volume it or replace filling tube
is too low Filling valve is loose After cleaning the dispenser (page 6),
or damaged tighten the valves using the service tool.
If necessary, replace filling valves
Leaking liquid
between Recirculation tube not Connect recirculation tube
dispenser connected
and bottle
10

Recommended Reagents
Below is a list of compatible reagents for use with this dispenser:
Aceta I dehyde Butyl methyl ether
Acetic acid {glacial), 100% Butyl amine
Acetic acid, S96% Butyric acid
Acetic an hydride Calcium carbonate
Acetone Calcium chloride
Acetonitri I
e Calcium hydroxide
Acetophenone Ca I ci um hypochl orite
Acetyl chloride Carbon tetrachloride
Acetyl acetone Chloro naphthalene
Acrylic acid Chloroacetaldehyde, :545%
Acrylonitrile Chloroacetic acid
Adipic acid Chi oroa cetone
Allyl alcohol Chi orobenzene
Aluminium chloride Chi orobuta ne
Amino acids Chloroform
Ammonia, :s; 20% Chlorosulfonic acid
Ammonia, 20-30% Chromic acid, :550%
Ammonium chloride Chromosulfuric acid
Ammonium fluoride Copper sulfate
Ammonium sulfate Cresol
n-Amyl acetate Cumene {lsopropyl benzene)
Amyl a I cohol {Penta nol) Cyclohexane
Amyl chloride {Chloropentane) Cycl ohexa none
Aniline Cyclopentane
Barium chloride Decane
Benzaldehyde 1-Decanol
Benzene {Benzol) Di benzyl ether
Benzine {Petroleum benzine) Dichloroacetic acid
bp 70-180 °C Di chi orobenzene
Benzoyl chloride Di chi oroetha ne
Benzyl a I cohol Di chi oroethyl ene
Benzylamine Di chi orometha ne
Benzylchloride Diesel oi I {Heating oi I),
Boric acid, S10% bp 250-350 °C
Bromobenzene Diethanolamine
Bromona phtha I ene Di ethyl ether
Butanediol Diethylamine
1-Butanol 1.2 Di ethyl benzene
n-Butyl acetate Di ethylene glycol
11

Recommended Reagents
Dimethyl sulfoxide (DMSO) Methyl formate
Di methyl a ni Ii ne Methyl propyl ketone
Dimethylformamide (DMF) Methylene chi ori de
1.4 Dioxane Mi nera I oi I (Engine oi I)
Di phenyl ether Monochloroacetic acid
Essenti a I oi I Nitric acid, :5 30%
Ethanol Nitrobenzene
Ethanol amine Oleic acid
Ethyl acetate Oxalic acid
Ethyl benzene n-Pentane
Ethylene chloride Peracetic acid
Fluoroacetic acid Perchloric acid
Forma I
dehyde, :5 40% Perch I oroethyl ene
Formamide Petroleum, bp 180-220 °C
Formic acid, :5100% Petroleum ether, bp 40-70 °C
Glycerol Phenol
Glycol (Ethylene glycol) Phenyl ethanol
Glycolic acid, :550% Phenyl hydrazine
Heating oil (Diesel oil), Phosphoric acid, :585%
bp 250-350 °C Phosphoric acid, 85%
Heptane Sulfuric acid, 98% 1:1
Hexane Piperidine
Hexanoic acid Potassium chloride
Hexanol Potassium di chromate
Hydriodic acid, :557% Potassium hydroxide
Hydrobromic acid Potassium permanganate
Hydrochloric acid, :520% Propionic acid
Hydrochloric acid, 20-37% Propylene glycol (Propanediol)
Hydrogen peroxide,:535% Pyridine
lsoamyl alcohol Pyruvic acid
lsobutanol Sa Ii cyl a I dehyde
lsooctane Scintilation fluid
lsopropanol (2-Propanol) Silver acetate
lsopropyl ether Silver nitrate
Lactic acid Sodium acetate
Methanol Sodium chloride
Methoxybenzene Sodium di chromate
Methyl benzoate Sodium fluoride
Methyl butyl ether Sodium hydroxide, :530%
Methyl ethyl ketone Sodium hypochl orite
12

Recommended Reagents
Sulfuric acid, S98%
Tartaric acid
Tetra chi oroethyl ene
Tetramethylammonium hydroxide
Toluene
Trichloroacetic acid
Tri chi orobenzene
Tri chi oroetha ne
Tri chi oroethylene
Tri chi orotrifl uoro ethane
Tri ethanol amine
Tri ethylene glycol
Trifluoro ethane
Trifluoroacetic acid (TFA)
Turpentine
Urea
Xylene
Zinc chloride, S10%
Zinc sulfate, S10%
_A CAUTION:-
Always follow all instructions listed in this manual as well as any recommendations from the reagent
manufacturer. In addition to the reagents listed in this manual, a variety of organic and inorganic
saline solutions (e.g. biological buffers), biological detergents and media for cell culture can be
dispensed. If used with strong acids, it is advised to rinse & remove dispenser at the end of each day
and store safely. If additional information is required please contact Globe Scientific.
13

diamond SureFlow
TM
Laboratory Bottle Top Dispenser
• Fully Autoclavable
• 2-YearWarranty
• Locking Feature Prevents Spills
• Unique Piston and Valve Designs
• 360° Rotating Dispenser Arm
• Telescopic Fill Tube