DMT 820MS User manual

USER MANUAL, VOL.3.2
MYOGRAPH SYSTEM - 820MS

PowerLab® and LabChart® are registered trademarks of ADInstruments Pty Ltd.
The names of specic recording units, such as PowerLab 4/35, are trademarks of ADInstruments Pty Ltd.
Pentium is a registered trademark of the Intel Corporation. Windows 7 and Windows 10 are registered trademarks
of Microsoft Corporation.
All other trademarks are the properties of their respective owners.
DMT reserves the right to alter specications as required.
This document was, as far as possible, accurate at the time of printing.
Changes may have been made to the software and hardware it describes since then.
New information may be supplied separately.
This documentation is provided with the DMT Muscle Strip Myograph System – Model 820MS
Document Number: 820MS – UG2.2A
All rights reserved. No part of this manual may be reproduced or transmitted in any form or by any means without
the written permission of Danish Myo Technology A/S.
Every attempt is made to ensure accurate information, misprints, construction- and specication changes can occur.
Danish Myo Technology A/S reserves the right to alter/change content as required and without any notice.
Copyright © Danish Myo Technology A/S
TRADEMARKS

CONTENTS
TRADEMARKS 3
SAFETY 5
UNPACKING THE MYOGRAPH SYSTEM 6
EC DECLARATION OF CONFORMITY 7
CHAPTER 1 - SYSTEM OVERVIEW 8
1.1 Interface 8
1.2 Interface Rear Panel 9
1.3 MyoDynamics Muscle Strip Myograph - 840MD 10
CHAPTER 2 - SETTING UP 11
2.1 Setting up the Muscle Strip Myograph System - 820MS 11
2.2 Setting up step-by-step 12
CHAPTER 3 - THE INTERFACE MENUS 14
3.1 Interface Menus - Navigation 14
3.2 Interface Menus – Calibrated Mode 15
CHAPTER 4 - THE MUSCLE STRIP MYOGRAPH UNIT 27
4.1 Calibration of the Force Transducer 27
4.2 Checking the Force Transducer 29
4.3 Force Transducer Replacement 30
4.4 Myograph Maintenance 32
4.5 Cleaning the Myograph 33
CHAPTER 5 - THE MOUNTING SUPPORTS AND MOUNTING IN THE 820MS CHAMBER 36
APPENDIX 1 - SYSTEM SPECIFICATIONS 38

5
The Muscle Strip Myograph System - 820MS has been designed for use in teaching and research only. It is not
intended for clinical or critical life-care use and should never be used for these purposes. The 820MS also should not
be used for the prevention, diagnosis, curing, treatment, or alleviation of disease, injury, or handicap.
CAUTION:
• Do not open the unit; the internal electronics pose a risk of electric shock.
• Do not use this apparatus near water.
• To reduce the risk of re or electric shock, do not expose this apparatus to rain or moisture. Objects
lled with liquids should not be placed on the apparatus.
• Do not block any ventilation openings. Install in accordance with the manufacturer’s instructions.
• Do not install near any heat sources such as radiators, heat registers, stoves, or other equipment or
devices that produce heat.
• Only use attachments and accessories specied by the manufacturer.
• Unplug this apparatus during lightning storms or when unused for long periods of time.
• Be advised that dierent operating voltages require the use of dierent types of line cord and
attachment plugs. Check the voltage in your area and use the correct type. See the table below:
SAFETY
Protect the power cord from being walked on or pinched: particularly at power plugs and the point where they
connect to the apparatus.
Refer all servicing to qualied service personnel. Servicing is required when the apparatus has been damaged in
any way; such as, the power-supply cord or plug is damaged, liquid has spilled onto or objects have fallen into the
apparatus, the apparatus has been exposed to rain or moisture, does not operate normally, or has been dropped.
Voltage Line plug according to standard
110 - 125 V UL81 and CSA C22.2 No. 42
220 - 230 V CEE 7 page VII, SR section 107-2-D1/IEC 83, page C4
240 V BS 1363 of 1984. Specication for 13A fused plugs and
switched and unswitched socket outlets.

6
UNPACKING THE MYOGRAPH SYSTEM
Take a few minutes to carefully inspect your new Muscle Strip Myograph System - 820MS for damage which may have
occurred during handling and shipping. If you suspect any kind of damage, please contact DMT immediately and the
matter will be pursued soon as possible. If the packing material appears damaged, please retain it until a possible
claim has been settled.
We recommend that you store the packing material for any possible future transport of the Muscle Strip Myograph
System. In case of transport and the original packing material is unavailable, please contact DMT Sales Department
for advice and packing instructions.
After unpacking your new Myograph System, please use the following list to check that the system is complete:
1 x interface unit
4 x chamber units with mounted clamp supports
4 x chamber covers
1 x external temperature probe
1 x power cord*
1 x calibration kit (including bridge, balance and 2 g weight)
1 x tube of high vacuum grease
1 x tube of grease for screw thread
2 x Allen keys
1 x small screwdriver
1 x USB with an user manual for Muscle Strip Myograph System
* The shape of the AC plug varies by country; be sure that the plug ts the outlets for your location.

7
EC DECLARATION OF CONFORMITY
DMT A/S
Certify and declare that the following apparatus:
MUSCLE STRIP MYOGRAPH SYSTEM - MODEL 820MS
Restrictive use: Only for laboratory use.
Manufactured by:
DMT A/S
Skejbyparken 152
8200 Aarhus N.
Denmark
Conforms with the essential requirements of the EMC Directive 2004/108/EC.
Based on the following specications applied by:
EN 61326-1:2006
EN 61326-2-6:2006
EN 61326-2-6/Corr.:2007
And with the LVD Directive 2006/95/EC.
Based on the following specications applied by:
EN 61010-1:2010
EN 61010-2-030:2010
General warnings regarding EMC:
Do not use this device in close proximity to sources of strong electromagnetic radiation
(e.g. unshielded intentional RF sources), as these may interfere with the proper operation.

8
CHAPTER 1 - SYSTEM OVERVIEW
Figure 1.1 Interface Front Panel
Myo-Interface display Valve buttons
Heat indicator
Power indicator
1.1 INTERFACE

9
Figure 1.2 Interface Rear Panel
1.2 INTERFACE REAR PANEL
Gas regulator
(needle valve)
Gas input
4 Transducer connections
pH meter connection
Temperature probe
Connection
4 Recorder outputs
(Analogue)
USB output (Digital)
ON/OFF switch
Power connector
Vacuum input
Grounding,
connected to Data
Acquisition

10
Connection to Myo-Interface
Micrometer
Myograph clamp support
connected to micrometer
Myograph clamp support
connected to force transducer
Force transducer pin
Figure 1.4 Close up of myograph unit
1.3 MYODYNAMICS MUSCLE STRIP MYOGRAPH - 840MD
Figure 1.3 MyoDynamics Muscle Strip Myograph unit

11
CHAPTER 2 - SETTING UP
2.1 SETTING UP THE MUSCLE STRIP MYOGRAPH SYSTEM - 820MS
This chapter contains a complete step-by-step description of how to set up a complete Muscle Strip Myograph System
- 820MS as illustrated in gure 2.1 above.
Figure 2.1 The complete Muscle Strip Myograph System - 820MS
DMT CS4/CS8 Stimulator (optional)
PC data acquisition and
analysis software (optional)
Myo-Interface front panel
PowerLab data acquisition
system (optional)
BNC Cables
Connection to
oxygen supply
Vacuum pump
(optional)
Suction bottle
(optional)
Myo-Interface rear panel
USB. DMT Device enabler option

12
2.2 SETTING UP STEP-BY-STEP
1. Interface – PC Connection:
Data acquisition is possible either by connecting the 620M Interface directly to a PC with Labchart Pro and the
DMT Device enabler installed or through the BNC connectors and BNC cables from the 820M interface e.g. to
the Powerlab box and the Powerlab box is then connected using a USB cable to the computer with Labchart
installed(optional).
I. Direct PC Connection: Connect the 620M Interface using the applied USB cable directly to the PC with
Labchart Pro installed (version 8 or newer).
II. PowerLab (Optional): Connect the 820M Interface to the PowerLab unit using BNC cables. Connect Rec 1on
the Interface to Input 1on the Power Lab, Rec 2 to Input 2 etc. Connect the PowerLab unit to one of the USB-
ports on the PC using the USB cable delivered with the PowerLab system.Connect the Powerlab box using
the USB cable to the computer with Labchart installed.
2. Oxygen Supply:
Connect the gas supply (95% O2, 5% CO2 or 21% O2, 5% CO2, balance N2) with tubing running from the gas
supply to the gas inlet on the back of the Interface. Oxygen is supplied to the chambers by tubing attached to the
stainless steel vacuum pipe. The oxygen and vacuum tubing need to be inserted into the chamber in order to
aerate the heated buer. Needle valves on the back of the interface can be adjusted to regulate the amount of
bubbling that occurs. Turning the regulator clockwise increases the bubbling while turning it counter-clockwise
decreases the bubbling. Each regulator has a lock device attached that can be used when the desired bubbling
is achieved. See Figure 2.2.
This chapter contains a complete step-by-step description of how to set up a complete myograph 820M system as
illustrated in Figure 2.1.
3. Vacuum Connection:
The system has a built-in manifold with separate valves that allows each chamber to be drained individually.
After connecting the vacuum source at the back of the Interface, the vacuum pipes need to be inserted into the
chambers in order for this feature to work properly. The pipes are inserted into the chamber by gently pulling
NOTE: The needle valves need to be greased (using the grease for the linear slides – the brown
grease) and turned at regular intervals to prevent them from sticking or permanently freezing.

13
NOTE: When draining the chambers using the automatic vacuum function, press the appropriate
button for an additional 3-5 seconds after the initial emptying. This will help drain residual buer
and solutions retained in the tubing and valves.
Figure 2.2 Oxygen supply and suction connection
Gas
Suction
4. Chamber Covers: The chamber covers will help maintain the temperature and other buer conditions (gas
tension, pH) fairly constant. Holes in the chamber covers serve dierent purposes, and they are illustrated in
Figure 2.3A-B The slots allow the covers to be placed over the chamber around the support arms and gas/
vacuum tubes.
Figure 2.3A - Chamber cover Figure 2.4B - Chamber cover with built-in electrodes (optional item)
Prior to the shipment of the Multi Wire Myograph 820M System, has gone through two days of continuous testing,
including a nal force transducer calibration. However, DMT recommends that a new force transducer calibration
is performed before using the myograph system for the rst time. The force transducer calibration procedure is
described in detail in the FORCE CALIBRATION sub-menu under SETTINGS, as explained in Chapter 3.
up on the curved part of the pipe, turning it 90° counter- clockwise and gently lowering it into the chamber. A
chamber can then be emptied by pressing the corresponding numbered button. Pressing the “all” button will
empty all the chambers at the same time, see Figure 2.2.

14
CHAPTER 3 - THE INTERFACE MENUS
3.1 INTERFACE MENUS - NAVIGATION
Chapter 3 is a complete manual for the 820MS Interface. This chapter contains a detailed description of how to
navigate the touch-screen menus and how to use the special features of the 820MS myograph. The 820MS system
can be used in 2 dierent modes. The rst mode is the Calibrated Mode, where the signal from the transducers is
ltered and conditioned before the signal is routed to the BNC outputs in the back of the interface for collection by
a data acquisition system. The second mode is the Direct Mode, and the signals from the transducers in this mode
bypass the internal amplier and are routed directly to the NBC outputs for data acquisition. Dierent menu options
are available in each mode and will be described in more detail in this chapter.
Menus on the 820MS interface are all accessible by a touch screen. To
access a menu, simply touch the screen. A setting can be changed by
touching the “SELECT” icon on the screen corresponding the “SELECT”
icon on the touch screen corresponding to the desired channel to be
changed.
COPY PROTOCOL
SELECT
SELECT
SELECT
SELECT
From Chamber no. 1
COPY
NOW ENTER
Protocol no. 3
Protocol no. 3
To Chamber no. 2
The line to be modied will turn blue, indicating that the interface is
waiting for input. When “ALL” is chosen, all lines corresponding to all
4 channels will turn blue. Changing the numeric value for the chosen
parameter can be done by touching the up or down arrow keys.
Touch “ENTER” when the desired setting has been chosen. Touching
the “X” will exit the menu and automatically take the user to the
“ACTUAL FORCE” Display.
COPY PROTOCOL
SELECT
SELECT
SELECT
SELECT
From Chamber no. 1
COPY
NOW ENTER
Protocol no. 3
Protocol no. 3
To Chamber no. 2
CHAMBER 1 PROTOCOL NO. 1
1 Speed [mm/sec.] 6.0
2 Wait for Dig-IN1 ON
3 Wait short [msec.] 500
4 Motor revers [um] 1200
5 Wait long [sec.] 5

15
In calibrated mode, the internal amplier is engaged and signals from the transducer are ltered and conditioned
before the signal is sent to a data acquisition system via the BNC outputs on the rear of the interface. The total
voltage output from the BNC connectors is 2.5 volts.
POWER-UP SCREEN
After turning on the 820MS Interface, an “Introduction” screen
appears. The system is auto-calibrating the A/D converters while this
screen is displayed.
3.2 INTERFACE MENUS – CALIBRATED MODE
After a few seconds, the “ACTUAL FORCE” display will appear.
At any given time, if the force applied on any channel is out of range,
the force reading for the overloaded channel will turn yellow as a
warning.

16
Three menus are accessible from the default “Actual Force” screen or
display. These menus are: Zero, Heat, and Settings.
ZERO MENU
This menu is used to zero the output of the transducers. When using
a data acquisition program like LabChart by ADInstruments ®, using
this feature will reset the baseline of the chart traces without aecting
the calibrations or physically changing any pre-load tensions placed
on the mounted vessels. The channels can be changed individually by
pressing “SELECT” or all at once by pressing “ALL”. Pressing “ENTER”
will execute the zero function.
HEAT MENU
The heating unit and temperature are controlled from this menu. To
turn the heat on or change the preset temperature for the system,
access the temperature control menu. Pressing the “HEAT” key will
enter the menu and allow the user to change the default system
temperature, as well as turn the heat on or o. Pressing “DEFAULT”
will automatically reset the temperature setpoint to 37°C. Manually
change the temperature by pressing the up or down arrows.
Turn the heat on by touching “ON” and the icon will turn green.
Pressing the white “X” in the red box will send the user back to the
“ACTUAL FORCE” display.

17
SETTINGS MENU
The “Settings Menu” contains several sub-menus that can be accessed
to change functional aspects of the interface. These sub-menus
include:
1. FORCE CALIBRATION
2. pH CALIBRATION
Enter the FORCE CALIBRATION sub-menu to perform the calibration
procedure.
3. FORCE REC. OUTPUT
4. MEASUREMENT RANGE
5. INTERFACE SETTINGS
These are the options that are present when the system is in
Calibrated Mode. Dierent options will appear when the system is in
Direct Mode.
1. Force calibration
NOTE: everytime a force calibration is performed the
MEASUREMENT RANGE is set to default 200 mN. Set
measurement range after the force calibration.
Touch “SELECT” to initiate the calibration for the chosen chamber
and the text will turn blue. Touching “ENTER” will start the 6-step
procedure for calibrating the chosen force transducer.

18
The calibration procedure is listed in 6 individual steps and needs to
be performed for each channel or transducer when calibrating the
system.
Step 1 involves setting up the chamber for calibration. Make sure the
chamber contains the clamp support. Fill the chamber with double-
distilled water for the volume to be used experimentally. Press “NEXT
STEP”.
Step 2 involves setting up the calibration kit appropriately for the
actual weight calibration. Verify that the transducer arm pin does not
touch the clamp support, as instructed. The pin should be as close as
possible to the clamp support or mounting pin without touching in
order to get the most accurate calibration. Press “NEXT STEP” when
the calibration kit has been properly placed.
Step 3 Initiates the heating process for the chambers. In order to
perform an accurate calibration the transducers must be heated to
the desired experimental temperature to accommodate heat-induced
expansion of the electronic parts in the transducer. Otherwise
inaccurate readings and transducer drift may occur.
To start heating, press “HEAT ON”. Using the chamber covers will
expedite the heating. Place the temperature probe into the rst
chamber to be calibrated to monitor the temperature. Heating will
take approximately 20-30 minutes to reach 37°C. Press “NEXT STEP”
when the chamber(s) has reached the target temperature.

19
Step 4 is the rst step in the actual weight calibration process. A
4-digit number will be displayed in blue at the bottom of the screen.
If nothing has been perturbed during the heating process, the zero,
0 gram, or 0.00 mN calibration should be stable as indicated by the
4-digit number and “NEXT STEP” can be pressed after 30-40 seconds.
If the 4-digit number is not stable, then wait until the number has
stopped uctuating before pressing “NEXT STEP”.
Step 5 is the 2 gram weight calibration. Place the 2 gram weight in
the pan closest to the transducer as to simulate a mounted muscle
pulling on the clamp attached to the transducer. Remember, a 2 gram
weight in a 90° vector is cut in half, and the transducer will only detect
1 gram or 9.81 mN of force. The weight placement should cause a
positive increase in the 4-digit number. Wait at least 30 to 40 seconds
for the applied force to stabilize before pressing “NEXT STEP”. Once
the 4-digit number has stabilized, press “NEXT STEP”.
Step 6 is to verify that the calibration was performed correctly. The
“Force Chamber 1” reading should be 9.81 ± 0.1 mN. If the “Force
Chamber 1” reading is o by more than 0.1 mN, then remove the
weight, press “BACK” to return to Step 4, and repeat the calibration
process. If the “Force Chamber 1” reading is satisfactory, then press
“NEXT STEP”. Calibrate the other chambers in the same manner.
2. pH Calibration Menu (Optional)
The pH Module in the 820M is locked by a code. If the screen shown
turn up, a code is needed from DMT to unlock the pH module.
By ordering the pH sensor from DMT the pH sensor is delivered with
the unlocking code and a quick guide how to do it. Entering the code
will open for the pH Calibration menu.

20
The 820M system has a build-in pH meter and a pH-meter electrode
plug-in port marked PH on the back side of the 820M interface.
The pH electrode can be ordered at DMT by contacting your sales
representative or emailing [email protected].
The pH calibration procedure is listed in 4 individual steps and needs
to be performed one at a time.
LOGINCODETOpH
CallDMTA/Stogetaccescode.
SystemIDnumber.1056
CLR ENTER
0
12345
6 7 8 9
  

Stepno.:
FollowthepHcalibration
procedureintheUserManual.

NEXTSTEP
1 2 3 4
CleanthepHelectrodeandthe
pHCALIBRATION
temperatureprobe.
Whenreadygotonextstep.
Step 1 - Step 1 involves cleaning the pH electrode and the temperature
probe with double distilled water. When ready Press NEXT STEP.
NOTE: Before the pH calibration is performed be sure to
select the way the pH electrode is to be used. See the sub-
menu pH Set-up under Interface Settings.
Step 2 - Place the pH electrode and temperature probe in the high
buer solution (here pH 7) and turn on stirring of the high buer
solution. When the relative pH output in the blue line is stable, go to
NEXT STEP.
Step 3 - Place the pH electrode and temperature probe in the low
buer solution (here pH 4) and turn on stirring of the low buer
solution. When the relative pH output in the blue line is stable, go
to NEXT STEP.
Stepno.:
ApplybufferwiththeHighpHvalue
tothepHandtemp.probe.

NEXTSTEPBACK
1 2 3 4
WhenthepHreading
pHreadings:1863
pHCALIBRATION
isstable,gotonextstep.

Stepno.:
ApplybufferwiththeLowpHvalue
tothepHandtemp.probe.

NEXTSTEPBACK
1 2 3 4
WhenthepHreading
pHreadings:883
pHCALIBRATION
isstable,gotonextstep.
