Dolomite MITOS P-PUMP REMOTE CHAMBER 400 User manual

MITOS P-PUMP
REMOTE CHAMBER 400
USER INSTRUCTIONS

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Contents
1Mitos 400 Safety Information 5
2System Set-up 7
2.1 Introduction 7
2.2 Using the Mitos P-Pump Remote Chamber 8
3Mitos P-Pump Control 11
3.1 Control 11

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Product name
Product picture
Product description
Mitos P-Pump
(Part No. 3200016)
The Mitos P-Pump provides pulseless liquid
flow with a precise pressure driven pumping
mechanism. It operates over a wide pressure
range (0-10bar) with excellent response time
and accuracy.
Mitos P-Pump Vessel
Holder Kit
( Part No. 3200017)
This kit contains a selection of vessels to
enable sample liquids to be introduced into the
Mitos P-Pump. It also contains a selection of
tubing and fittings to enable fluidic connection
to be made between the Mitos P-Pump and a
microfluidic system.
Flow Resistors, F1, F3,
F10, F30, F100
(Part No. 3200028–
3200032)
The Flow Resistor can be used to insert
additional flow resistance into a system. It
enables very low flow rates to be achieved
when connecting the P-Pump to systems of low
fluidic resistance.
Mitos P-Pump
Starter Kit
(Part No. 3200033)
This kit contains a selection of tubing and
fittings to enable fluidic connection to be made
between the Mitos P-Pump and a microfluidic
system.
Pneumatic Connector Kit
(Part No. 3200034)
This kit contains a selection of fittings and
tubing to enable connection of your
compressed air or gas supply to the Mitos P-
Pump.
Mitos P-Pump product range

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Mitos P-Pump
3-way Chamber Lid
(Part No. 3200044)
The Mitos P-Pump 3-way Chamber Lid can be
used to pump 3 liquids simultaneously from the
Mitos P-Pump. This provides a low cost method
of delivering 3 reagents to a microfluidic system
with pulseless pressure pump technology.
Mitos P-Pump 3-way
Vessel Holder Kit
(Part No. 3200045)
This Vessel Holder is designed to
accommodate 3 micro tubes in the Mitos P-
Pump. Included in the kit are compatible micro
tubes with 1.5ml volume. When used in
conjunction with the Mitos P-Pump 3-way
Chamber Lid, it provides a low cost method of
delivering 3 reagents to a microfluidic system
with pulseless pressure pump technology.
Mitos P-Pump Remote
Chamber 400
(Part No. 3200043)
The Remote Chamber enables greater input
volumes up to 400 ml to be used with the Mitos
P-Pump. Continuous pumping for long periods
of time is therefore possible.

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1 Mitos 400 Safety Information
Maximum Allowable Pressure = 10 bar gauge.
Do not connect a pressure supply over 10 bar gauge to the Mitos 400
remote chamber. Do not apply a negative pressure (vacuum) to the remote
chamber. Do not subject the remote chamber to sudden changes in
pressure. Failure to observe these requirements may result in failure of the
remote chamber and injury to the user. A supply pressure of no more than 11 bar gauge
may be connected to the P-Pump that feeds the remote chamber.
Glass Pressure Chamber Warning
If the glass pressure chamber is scratched or cracked, do not continue to
use. This may result in failure of the Remote Chamber and injury to the
user. Do not use without the safety screen in place. Please contact
Dolomite for replacement of the glass vessel.
Maximum Allowable Temperature Range 0-50°C
Do not subject the remote chamber to internal or external temperatures
over 50°C or below 0°C. The remote chamber may be used on a hotplate
providing the plate surface temperature does not exceed 50°C.
Tightening Torques
Tighten remote chamber lid thumbscrews and fluid fittings firmly but finger
tight only. Do not use tools to tighten
Fluid Vessel Warning
Do not insert the fluid vessel supplied with cap on (or any other sealed
container) into the chamber. This may result in failure of the P-Pump
Remote Chamber 400 and injury to the user.
General Advice on Use of Pressure Equipment
Care must be taken when the P-Pump and Remote Chamber is
pressurised to ensure that the unit is not dropped or damaged in any way.
Mounting holes have been provided to fix the unit to the bench. The
chamber is not designed to accommodate blast damage, flame
impingement or other external hazard. Fluids should not be allowed to
condense on the chamber walls. Do not attempt to open the remote
chamber when under pressure.

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Use of Oxygen and flammable gases
The remote chamber should only be used with class 2 fluids. The user is
responsible for ensuring the chamber is suitable for use with the intended
fluids. The chamber should not be used with Oxygen or flammable gases.
Compatible fluids/gases
User must ensure that all gasses and fluids used with the remote chamber
are compatible with the materials used in the construction of the
chamber. The chamber must not be used with substances and mixtures,
as defined in points (7) and (8) of Article 2 of Regulation (EC) No
1272/2008, which include, but are not limited to, flammable or oxidising
gasses; such as Oxygen or acetylene, toxic or corrosive fluids.
Mitos 400 Safety Information
The Mitos 400 remote chamber is manufactured and tested in accordance
with Article 4, paragraph 3 of the pressure equipment directive 2014/68/EU
and is classified under ‘Sound Engineering Practice’. The user is
responsible for ensuring the Mitos 400 remote chamber is installed,
operated and maintained safely by following the installation, maintenance
and operating instructions specified in this manual and is suitable for use
with the intended fluid.
If the Mitos 400 is fitted to a system with a higher temperature and
pressure capability than the limits specified in this manual, or where
malfunction could lead to over-pressure or over-temperature, then the user
must ensure a safety device (such as a burst disc) is fitted to prevent
occurrence of a dangerous condition.
Eye Protection
Safety glasses and additional appropriate PPE should be worn at all times
when using the remote chamber.

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2 System Set-up
2.1 Introduction
The Mitos P-Pump Remote Chamber is connected to the Mitos P-pump, which precisely
controls the pressure inside both chambers. In this mode of operation, the liquid vessel is
placed inside the Mitos P-Pump Remote Chamber.
The user sets the chamber pressure (gauge pressure) on the Mitos P-Pump, which
determines the flow rate of liquid from the Remote Chamber. When the user vents (or
when the Mitos P-Pump is switched off) the pressure inside both chambers returns to
atmospheric and flow stops.
Gauge pressure: The pressure relative to atmospheric pressure (ambient air).
Absolute pressure: The pressure relative to absolute zero (perfect vacuum).
gas flow
liquid
flow
Mitos P-Pump
chamber pressure
is equal to Remote
Chamber pressure
atmospheric
pressure
gas
supply
gas
flow

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2.2 Using the Mitos P-Pump Remote Chamber
Check that you have the following
components:
•Remote chamber
•Remote chamber lid with dip
tube fitting
•Pneumatic connector
•250ml Duran bottle
•Mitos P-Pump (not included)
•Compressed gas or air supply
(not included)
Step 1:
Unscrew the 4 thumb screws in the
remote chamber lid.
Step 2:
Remove lid from remote chamber.
Step 3:
Fill vessel with sample liquid.
remote chamber
remote chamber lid
pneumatic connector
Duran bottle

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Step 4:
Place liquid vessel inside remote
chamber.
DO NOT PLACE LID ON LIQUID
VESSEL
This is important to prevent pressure
chamber failure and injury to the user.
Step 5:
Place lid on remote chamber and
tighten the 4 thumb screws evenly.
Do not completely tighten one thumb
screw before tightening the others as
this will result in uneven clamping
force.
Ensure that all thumb screws are fully
tightened.
Step 6:
Check that dip tube fitting is
unscrewed then insert dip tube
through until it reaches the bottom of
the liquid vessel.
Tighten dip tube fitting to make a seal
around the tube.
Push tube into cleat on dip tube fitting
to retain.

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Step 7:
Use the pneumatic connector supplied
to connect the Remote Chamber to
the Mitos P-Pump.
Step 8:
Set pressure on Mitos P-Pump (see
Mitos P-Pump user instructions) to
start pumping.
To stop pumping, vent the chamber by
pressing the back button on the Mitos
P-Pump.
Do not vent the chamber by
unscrewing the lid or fittings.
Press
to vent