Drummond Nanoject III User manual

Nanoject III
Programmable Nanoliter Injector
MADE IN USA
INSTRUCTION MANUAL • 3-000-207
LABORATORY EQUIPMENT
E21997

2
• For indoor use only.
• Never operate unit in an explosive atmosphere.
• Do not operate unit with a damaged ord.
• Use power sour e only in a standard ele tri al outlet.
• Do not handle power sour e with wet hands.
• Do not put unit or power sour e in water or other liquid.
• When servi ing, use only identi al Drummond repla ement parts.
• Save these instru tions.
If the equipment is used in a manner not specified by the manufacturer,
the protection provided by the equipment may be impaired. Always use
the power source that is supplied with the unit.
Speci ications or Usage
This unit is intended to be used to inject nanoliter quantities of sample.
This equipment is or indoor use only.
Temperature Range 10°C–35°C, Maximum Humidity 60%
FAILURE TO USE THE EQUIPMENT IN ACCORDANCE WITH INSTRUCTIONS
OR MODIFYING THE EQUIPMENT WILL VOID WARRANTIES.
CAUTION: CAREFULLY READ THROUGH THIS ENTIRE MANUAL BEFORE USING
YOUR NEW NANOJECT III. PAY CLOSE ATTENTION TO THE RULES
FOR SAFE OPERATION WARNINGS AND CAUTIONS.
Rules or Sa e Operation
Connections to Power Source:
Che k the power sour e to see that the line voltage orresponds to the voltage
indi ated on the mains adapter.
If the mains adapter sour e and the line voltage are not ompatible, the ele tri al
omponents of the Nanoje t III may be damaged or destroyed.
Before ea h use, onfirm that the mains adapter able is not damaged, worn or
severely bu kled and there are no breaks in the insulating surfa e. If any damage is
noted, do not use the Nanoje t III until the damaged mains is repla ed.
Connecting to power outlet: The ontrol box has a power sour e transformer
atta hed to the box. Plug this into your outlet using the orre t adapter supplied.
There is an “on/off” swit h on the side of the ontrol box whi h turns on the ontrol
box on e the inje tor head is atta hed. To turn off, use the swit h to turn off. If you
want to dis onne t the power from the unit, merely unplug the transformer from the
wall outlet.
Power/Current Rating: AC 100~V, 50-60 Hz, 38VA DC9V 2A
Input: 100-240~V, 50-60 Hz, 0.6A
Output: 9V 2A

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Quick Start Guide
1) Carefully insert either end of the able into the plug on the top of the inje tor head.
Plug the opposite end of the able into the “HEAD” port on the right side of the
ontrol box.
2) If using the footswit h, insert the footswit h able into the “FOOTSWITCH” port on
the right hand side of the ontrol box.
3) Plug in the ontrol box into a standard ele tri al outlet using the appropriate
adapter head on the power supply.
4) Turn on unit with the on/off swit h lo ated on the left hand side of the ontrol box.
5) Initially the Drummond logo will be displayed on the s reen and then the operational
mode s reen will appear with the following i ons:
Sele t the manual mode by pressing the
[ANUAL] i on.
Note: During this brief startup phase,
the inje tor head will automati ally drive
the plunger to its fully retra ted position
(“home”) and the ontrol box will emit an
audible beep upon ompletion.
6) Install a mi ropipet onto the inje tor
plunger by first loosening the bla k ollet slightly. Ba kfill your mi ropipet with a
suitable lightweight mineral oil (or any other non- ompressible liquid), and slide it
onto the wire plunger until it seats firmly, then tighten the ollet. It works best if the
wire plunger is extended slightly so you an see what you are doing.
NOTE: THE ICROPIPET UST BE FILLED CO PLETELY WITH SO E TYPE OF
BACKFILLING SOLUTION. THE UNIT WILL NOT INJECT ACCURATELY WITH AIR
INSIDE THE ICROPIPET–THIS INCLUDES AIR BUBBLES ALSO.
On e the mi ropipet is ba kfilled with oil and se ured, press the [E PTY] i on
down until the plunger is fully extended (approximately 23 mm beyond the end of
the bla k ollet). A single beep will be heard when the plunger is fully extended.
(This will for e the ba kfilling solution to the tip of the mi ropipet and any ex ess
will be expelled).
7) Front-fill the mi ropipet with your sample by pla ing the mi ropipet tip into your
sample and pressing the [FILL] i on. You may want to fill at a slow speed or fill by
alternately pressing the [FILL] i on for a few se onds and then the [STOP] i on to
allow the sample to equilibrate before pressing the [FILL] again. This is dependent
on the tip size and the vis osity of your sample.
Note: The plunger will ontinue to extend or retra t until you press the [STOP]
i on, or a fully extended or fully retra ted position is rea hed.
8) To inje t the sample, return to the operational mode s reen by pressing the [EXIT]
i on, then sele t inje t mode by pressing the [INJECT] i on. Set the desired
inje tion volume (NL) and rate using the respe tive [+] and [-] i ons. Press the
[INJECT] i on to inje t your sample.
Note: Multiple inje tions an be performed by simply pressing the [INJECT] i on
again and again.

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[ANUAL]ode
This mode will enable the user to
manually fill and empty the mi ropipet.
Pressing the [FILL] i on will retra t the
wire plunger, while pressing the [E PTY]
i on will ause the wire plunger to extend.
When the [HO E] i on is pressed, the
wire plunger will fully retra t to the
“home” position and should be flush with
the end of the bla k ollet.
Operational Modes
[INJECT]ode
This mode is used to do single manual
inje tions. It enables the user to program
an inje tion volume (0.6 – 999.9nL) and
an inje tion rate (nL/se ) using the
respe tive [+] and [-] i ons. Press the
[INJECT] i on to inje t the desired
volume at the rate sele ted.
To the right of the inje t i on, you will
see a ounter box. This will ount the
number of inje tions. You an hange volume and/or rate and the ounter will ontinue
to ount. If you hange s reens, the ounter will zero out. You will also see an [RST]
i on that when pressed, will reset the ounter to zero.
Note: Multiple inje tions an be performed by simply pressing the [INJECT] i on again
and again. The inje tion rate is independent of the empty rate as programmed in the
[SETUP] mode.
Press the [DONE] i on to exit ba k to the operational mode s reen.
Note: The [HO E] and [EXIT] i ons
are disabled while the wire plunger is
in motion.
Note: The speed of the fill and empty
movement an be regulated in the
[SETUP] mode and is independent of
the inje tion rate.
Press the [EXIT] i on to exit ba k to
the operational mode s reen.
If the [FILL] or [E PTY] i on has been pressed and you desire to stop the a tion,
simply press the same i on position (now labeled [STOP]) a se ond time.

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[PROGRA ]ode
This mode enables the user to
program multiple inje tion y le
re ipes. To program a new re ipe,
press the [NEW] i on. A s reen
displaying the sample inje tion volume
(nL) and an inje tion rate (nL/se ) will
appear. As before, adjust these
individual values by using the
respe tive [+] and [-] i ons.
Below the [RUN] i on is a box displaying the number of inje tion y les remaining in
the entire program re ipe. It will ount down toward (0) as the multiple inje tion y le
progresses. Below this “ ounter” box is a display of the per entage of the program re ipe
remaining. It will ount down toward (0%) as the multiple inje tion y le progresses.
Press the [EXIT] i on to exit ba k to the operational mode s reen.
Pressing the [NEXT] i on brings up a s reen displaying the number of inje tion
y les. Again, use the [+] and [-] i ons to sele t the value desired.
Pressing the [BACK] i on always returns you to the previously displayed s reen.
Pressing the [NEXT] i on on e again brings up the s reen displaying the interval
(se s) between inje tion y les. Use the [+] and [-] i ons to set the desired time.
Pressing the [NEXT] i on a final time allows you to view the entire program re ipe
values in the following format:
VOL (NL) . . . . .volume of inje tion
RATE . . . . . . . .rate of inje tion
CYCLES . . . . .number of y les this volume will be inje ted
TI E (SEC) . . .time interval between ea h inje tion
Note: The program re ipes are automati ally saved and labelled in sequential order.
You an step through the stored re ipes by pressing the [<] and [>] i ons. Should you
desire to delete a re ipe, simply press the [DEL] i on, and the displayed re ipe will be
deleted.
Pressing the [RUN] i on will initiate the urrently displayed program re ipe.
After pressing the [RUN] i on, a [PAUSE]
i on appears and enables the user to
interrupt the program at any time, and
then resume the program where it was
interrupted by pressing the [RUN] i on
again.
To initiate a single y le of the displayed
re ipe, simply press the [TEST] i on on e.

6
Notes
The Nanoje t III does not use any O-rings to se ure the mi ropipet. We have in orporated
a small hu k and a sili on re eiver for the glass mi ropipet. The mi ropipet should slide
easily onto the wire, provided the ollet has been loosened enough.
When storing the inje tor, do not tighten the ollet. Leave it slightly loosened.
Cleaning Instructions: To lean the Nanoje t III, simply wipe the ontrol box down with
a moist loth. The inje tor head an also be leaned with a moist loth; uns rew the
ollet and wipe off the ex ess oil with a lean Kimwipe or soft loth. The wire plunger
may be wiped down with al ohol.
Wire Plunger
(3-000-000-207)
Collet
(3-000-030-C)
Chuck
Glass Micropipet
Seal
(Required
Orientation)
[SETUP]ode
This mode will enable the user to
program and store the manual fill and
empty rates. As before, use the [+]
and [-] i ons to sele t the desired
independent values for both the FILL
SPEED (NL/SEC) and the EMPTY SPEED
(NL/SEC).
Note: As mentioned earlier, the fill and
empty speeds on this s reen have no
effe t on the inje tion rate.
Press the [HO E] i on to manually fully retra t the plunger to the “home” position.
Press the [EXIT] i on to exit ba k to the operational mode s reen.
Orientation of the ollet omponents is riti al
to the proper operation of this unit. You will
see the ollet ontains a bla k “ hu k” and a
green “seal”. This seal has a small opening
and a large opening. The small opening or hole
slides over the wire plunger while the large
opening or hole re eives the ba k end of the
pulled mi ropipet and must fa e the tip of the
mi ropipet. Failure to orient this pie e properly
will ause an improper seal and will not enable
the Nanoje t III to inje t properly.
When pla ing the ba kfilled mi ropipet on to
the wire plunger and through the hu k, do not
for e the mi ropipet, merely push it onto the
plunger until it bottoms out in the green seal.
Then tighten the ollet to se ure the pipet.

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Speci ications
Part Number . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-000-207
Power Sour e . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100/240 volt, 50/60 Hz
Total Sample Volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.2 µL
Fill / Empty Volume Speed . . . . . . . . . . . . . . . . . . . . . . . . . . 10 nL - 200 nL/se
Inje tion Volume Range . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.6 nL - 999.9 nL
Inje tion Rate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 nL - 200 nL/se
Plunger Travel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 mm
Glass Mi ropipet Dimensions . . . . . . . . . . . . . . . . . . . . . . . . OD 0.045˝ (1.14 mm)
ID 0.021˝ (0.53 mm)
Control Box Dimensions
Weight: .43 kg
Length: 14 m
Width: 13.5 m
Thi kness: 4 m
Questions, Comments,
Suggestions or Service:
CALL OUR HOTLINE
AT 1-800-523-7480
Power Sour e supplied with four inter-
hangeable adapter plugs for use in
the U.S., UK, Europe, and Australia

©Copyright, Drummond S ientifi Company, 2016, Rev. 0416 Printed in USA
500 Parkway, Box 700
Broomall, PA 19008
800/523-7480 • FAX: [phone]
Website: www.drummonds i. om
For Te hni al Assistan e, all
1-800-523-7480 or e-mail
in [e-mail]
3-000-207 kit contains the ollowing items:
Control box & power sour e . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-000-037
Inje tor head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-000-030-B
Inje tor head able . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-000-031
100 p s., 3.5˝ glass apillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-000-203-G/X
100 p s., 7˝ glass apillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-000-203-G/XL
Ba kfilling needle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-000-027
Universal adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-000-024-A
Accessories not included in kit:
Repla ement ollet kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-000-030-K
(1 ollet, 1 hu k, 1 seal, 1 wire plunger, 1 Allen wren h)
Repla ement ollet only . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-000-030-C
Repla ement sili on seal (3 pk) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-000-030-S
Repla ement wire plunger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-000-000-207
(Note: Not the same as the Nanoje t II plunger)
Support base . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-000-025-SB
Mi romanipulator, right hand . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-000-024-R
Mi romanipulator, left hand . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-000-024-L
Footswit h . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-000-032
(remote apability for fill, empty and inje t fun tions)
Questions: Please onta t Chu k Lo ke at 800-523-7480 or
[email protected] if you need any assistan e.