Thermo Scientific CL10 User manual

IEC CL10 Centrifuge
User’s Manual
Analyze •Detect •Measure •ControlTM

IEC CL10 Centrifuge
IEC CL10 Centrifuge
User’s Manual

Carefully read this manual before operating your instrument.
Information contained in this document is the property of Thermo Electron Corporation; it may not be
duplicated or distribuited without the owner’s authorization.
The validity of the guarantee is subject to the observation of the instructions
and precautions described in this document.

Thermo Electron Corporation
MANUAL NUMBER
REV ECR/ECN DATE DESCRIPTION BY
a 11290085 03/06 Initial release
Preface
IEC CL10 i

ii IEC CL10 Thermo Electron Corporation
Packing List
11210900 Centrifuge IEC CL10 230V 50/60 Hz
11210901 Centrifuge IEC CL10 120V 60 Hz
11210902 Centrifuge IEC CL10 100V 50/60 Hz
Item number Quantity Check
Centrifuge 1 
User manual 1 
Manual lid opening instructions 1 
64227001 Mains cord 1 
Toolbox:
11202979 Rotor removal tool 1 
25535010 Emergency unlocking tool 1 
26387032 Spare fuses 2 
For missing parts call your nearest Thermo representative

IEC CL10 iiiThermo Electron Corporation
WEEE Compliance
WEEE Compliance. This products is required to comply with the
European Union’s Waste Electrical & Electronic Equipment (WEEE)
Directive 2002/96EC. It is marked with the following symbol. Thermo
Electron has contracted with one or more recycling/disposal companies
in each EU Member State, and this products should be disposed of or
recycling through them. Further information on Thermo Electron’s
compliance with these Directives, the recyclers in your country, and
information on Thermo Electron products which may assist the detec-
tion of substances subject to the RoHS Directive are available at
www.thermo.com/WEEERoHS
WEEE Konformittät. Dieses Produkt muss die EU Waste Electrical &
Electronic Equipment (WEEE) Richtlinie 2002/96ECerfüllen. Das
Produkt ist durch folgendes Symbol gekennzeichnet. Thermo Electron
hat Vereinbarungen getroffen mit Verwertungs-/Entsorgungsanlagen in
allen EU-Mitgliederstaaten und dieses Produkt muss durch diese
Firmen widerverwetet oder entsorgt werden. Mehr Informationen über
die Einhaltung dieser Anweisungen durch Thermo Electron, die
Verwerter und Hinweise die Ihnen nützlich sein können, die Thermo
Electron Produkte zu identizfizieren, die unter diese RoHS Anweisung
fallen, finden Sie unter www.thermo.com/WEEERoHS
Conformità WEEE. Questo prodotto deve rispondere alla direttiva
dell’Unione Europea 2002/96EC in merito ai Rifiuti degli Apparecchi
Elettrici ed Elettronici (WEEE). È marcato col seguente simbolo.
Thermo Electron ha stipulato contratti con una o diverse società di
riciclaggio/smaltimento in ognuno degli Stati Membri Europei. Questo
prodotto verrà smaltito o riciclato tramite queste medesime. Ulteriori
informazioni sulla conformità di Thermo Electron con queste
Direttive, l’elenco delle ditte di riciclaggio nel Vostro paese e informa-
zioni sui prodotti Thermo che possono essere utili alla rilevazione di
sostanze soggette alla Direttiva RoHS sono disponibili sul sito
www.thermo.com/WEEERoHS
WEEE. Este producto requiere cumplir con la Directiva 2002/96EC
WEEE (residuos de quipos electricos y electronicos). Este producto esta
marcado con el simbolo WEEE, como se describe. En cada Estado
Miembro de la Union Europea (EU), Thermo Electron ha contratado
con diversas companias para disponer y/o re-ciclar los residuos de equi-
pos electricos y electronicos. Existe information adictional acerca de la
conformidad de Thermo Electron con esta Directiva, incluyendo los
nombres de las diversas companias autorizadas para disponer/recyclar
nuestros productos. Adicionalmente suministramos informacion acerca
Great Britain
Deutschland
Italia
Espana

iv IEC CL10 Thermo Electron Corporation
WEEE Compliance
de los productos de Thermo Electron que puedes asistir en la deteccion
de substancian mensionades en la directiva RoHS, explicado en
www.thermo.com/WEEERoHS
Conformité WEEE. Ce produit doit être conforme à la directive euro-
péenne (2002/96EC) des Déchets d’Equipements Electriques et
Electroniques (DEEE). Il est merqué par le symbole suivant. Thermo
Electron s’est associé avec une ou plusieurs compagnies de recyclage
dans chaque état membre de l’union européenne et ce produit devrait
être collecté ou recyclé par celles-ci. Davantage d’informations sur la
conformité de Thermo Electron à ces directives, les recycleurs dans
votre pays et les informations sur les produits Thermo Electron qui
peuvent aider le détection des substances sujettes à la directive RoHS
sont disponibles sur www.thermo.com/WEEERoHS
France

IEC CL10 vThermo Electron Corporation
Declaration of compliance
We, Thermo Electron Industries S.A.S., Z.I. d’Aze Bellitourne 53200
CHATEAU-GONTIER, France, declare that the instrument defined
here below complies with the following European directives:
Nous, Thermo Electron Industries S.A.S., Z.I. d’Aze Bellitourne 53200
CHATEAU-GONTIER, France, déclarons que le matériel désigné ci-
après est conforme aux directives européennes suivantes:
Wir, Thermo Electron Industries S.A.S. , Z.I. d'Aze Bellitourne 53200,
CHATEAU-GONTIER, France, erklären dass das hier beschriebene
Gerät sich in Übereinstimmung mit folgenden europäischen
Richtlinien befindet:
Nostros, Thermo Electron Industries S.A.S., Z.I. d’Aze Bellitourne
53200 CHATEAU-GONTIER, France, declaramos que el instrumento
bajo descrito está fabricado según las especificaciones establecidas por
las siguientes normas europeas:
Nós, Thermo Electron Industries S.A.S., Z.I. d’Aze Bellitourne 53200
CHATEAU-GONTIER, France, declaramos que o instrumento aqui
apresentado está de acordo com as normas européias abaixo listadas:
Noi, Thermo Electron Industries S.A.S., Z.I. d’Aze Bellitourne 53200
CHATEAU-GONTIER, France, dichiariamo che lo strumento sotto
descritto è conforme alle seguenti Direttive europee:
• Machinery: 98 / 37 / CEE
• Low voltage: 72 / 23 / CEE - 93 / 68 CEE
• EMC: 89 / 336 CEE - 92 / 31 CEE - 93 / 68 CEE
• Safety standard: EN 61010-1 - EN 61010-2-020
Château-Gontier, 28th June 2005
Managing Director
Christian Galiana
Declaration de conformite
Declaration of compliance
Konformitätserklärung
Declaración de conformidad
Declaraçao de conformidade
Dichiarazione di conformità

vi IEC CL10 Thermo Electron Corporation
Guarantee Terms
Thermo guarantees that this unit is free from defects in materials and
workmanship when it leaves the factory, and will replace or repair the
unit if it proves defective in normal use or during service for a period of
ONE YEAR from the delivery.
This guarantee is invalid if the unit is incorrectly used, poorly serviced
or neglected, mis-used or accidentally damaged.
There is no explicit guarantee other than as stated above.

IEC CL10 viiThermo Electron Corporation
For further information,
assistance or service
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Other Europe, Middle East, or Africa
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In Other Countries:
Contact your local THERMO products distributor
or agent.
E-mail Technical Service Representative for
products at:
[email protected]
Visit our web site at: www.thermo.com

Contents
Manufacturer ..............................................................................................1-1
Advice to the user in safety terms......................................................1-2
Safety devices and notices..................................................................1-2
User Manual......................................................................................1-2
Warning markings ............................................................................1-2
Installation ..................................................................................................2-1
Transport, Packaging, Lifting. ..........................................................2-1
Installation ......................................................................................2-1
Mains Wiring....................................................................................2-1
Environmental conditions ................................................................2-1
Lid Unlock........................................................................................2-2
Emergency lid unlock ......................................................................2-2
Preliminary inspection ......................................................................2-3
Assembly of Rotating Equipment ....................................................2-4
Rotating head removing ..................................................................2-4
Characteristics............................................................................................3-1
Dimensions and Weight....................................................................3-1
Centrifugation characteristics ..........................................................3-1
Electrical characteristics ....................................................................3-1
Specifications ..................................................................................3-1
Description and Performance..................................................................4-1
Functional principles ........................................................................4-1
Drive system......................................................................................4-1
Safety interlock system ......................................................................4-1
Imbalance Sensor ..............................................................................4-1
Rotating equipment - Performance....................................................4-2
Rotating equipment - Table ..............................................................4-2
Performance ......................................................................................4-2
RCF Calculation ..............................................................................4-3
Front Panel........................................................................................4-4
Operation Procedure ................................................................................5-1
Instructions to the operator ..............................................................5-1
Power Switch - Cover unlock ............................................................5-1
Loading balance ..............................................................................5-1
SET parameters ................................................................................5-3
Acceleration and Deceleration. Soft key.............................................5-3
Chapter 1
Chapter 2
Chapter 3
Chapter 4
Chapter 5
viii IEC CL10 Thermo Electron Corporation

IEC CL10 ixThermo Electron Corporation
Contents
Setting and changing of rpm, min parameters ..................................5-4
Starting the centrifuge ......................................................................5-3
Stopping the centrifuge ....................................................................5-3
Messages - Alarms ............................................................................5-4
Hazards, Precautions and Limitation of Use ........................................6-1
Dangerous operations - working conditions to avoid ......................6-1
EN 61010-2-020 Standard (IEC 1010-2-020)..................................6-2
Rotor and accessory precautions ......................................................6-2
Corrosion information ....................................................................6-2
Contamination hazards ....................................................................6-5
Routine Maintenance ................................................................................7-1
Rotating Equipment and Centrifugation Chamber cleaning ............7-1
Exterior cleaning ..............................................................................7-2
Fuses ................................................................................................7-2
Chapter 6
Chapter 7

Chapter 1 Manufacturer
• This centrifuge was properly manufactured and is safe and reliable.
It could however become dangerous for the operator or for other
people if:
it is used by incompetent personnel or not according to the
attached instructions ▲
it is used for different purposes from the ones it was built for ▲
it is not correctly earthed ▲
• Normal use of the centrifuge could require the manipulation of
biological and hazardous materials. The users and personnel of
maintenance must have specific requirements of professionalism for
each substance they use; they must be trained to operate with
safety, as identified in the World Health Organization “Laboratory
Biosafety Manual”.
• The centrifuge cannot be electrically and/or mechanically
connected to other instruments.
• It is forbidden the use within the centrifuge of flammable or
explosive materials, as well as materials which chemically interact
vigorously.
• As this centrifuge does not conform to anti-deflagration standards,
it should be used exclusively in explosion proof areas.
• Maximum density of the centrifugation samples should not exceed
1200 kg/m3.
• The centrifuge should be used exclusively with rotating equipment
specified by instructions, in their integrity and only with original
accessories.
• Only original Thermo Electron spare parts can be used.
• It is strictly forbidden to take any changes to the centrifuge by
customers or other personnel not authorized.
• Do not use the centrifuge when it is damaged or not safely
operating or irregularly. Advice authorized personnel by
manufacturer as soon as for every anomaly.
Advice to the user in
safety terms
IEC CL10 1-1Thermo Electron Corporation

Chapter 1
Manufacturer
1-2 IEC CL10 Thermo Electron Corporation
In order to ensure both mechanical and electrical operational safety, the
IEC CL10 centrifuge is provided with the following safety control
systems, as detailed in the attached Declaration of Agreement:
• The centrifuge will not start unless the lid is locked.
• The lid cannot be opened during centrifugation, even in the event
of power failure.
• Safety electronic circuits for self-diagnosis (with micro - processor
control); any possible breakdown of circuits and sensors is shown
on display and neutralized at the same time, preventing unsafe
operating conditions of the machine.
• Load imbalance safety device.
• Double metal protection casing; reinforced centrifugation chamber
in AISI 304 stainless steel.
• Lid antifall device.
• Main bipolar switch.
• Individual certified test, with measurements and testing of leakage
currents, earth connection resistance and applied voltage.
• Identification plates; operating and maintenance instructions.
This manual is part of the centrifuge and it should be conserved for
future notes and consultations. Keep always the manual close to the
appliance and in a safe place, so that it is always available.
Thermo Electron invites all users to read this manual.
The following symbols are necessary for the operator to preserve the
protection afforded by the equipment and to preserve dangers.
On the main switch allows centrifuge operating. ▲
On the main switch disconnects the centrifuge from the main supply. ▲
This symbol means that you will find important information for safety
and if they are unobserved it could be taken some damages to appliance
and/or operator. ▲
Only authorized personnel can operate with the parts where there is the
symbol and after switching off the mains supply. To operate with high
voltage could cause death. ▲
Safety devices and
notices
User Manual
Warning markings

IEC CL10 2-1Thermo Electron Corporation
Transport, Packaging,
Lifting
Installation
Mains Wiring
Chapter 2 Insatllation
During transport centrifuge is seated in a special packaging.
All lifting and transporting must be carried out by using proper
handling equipment and by people having undergone the necessary
training.
1. General conditions accepted for centrifuge transport and storage are:
• Environment temperature -20°C + 50°C.
• Relative humidity up to 90% excluding moisture formation.
2. Unpack the centrifuge, carefully removing the rotating equipment,
any possible accessories and the material supplied for ordinary
maintenance. Check the contents of packaging against the packing
list.
(Keep the packaging until the centrifuge has been tested).
The machine should be installed in a dust and corrosion free room.
Place the centrifuge on a bench top, which must be rigid and
sufficiently strong.
Leave a 300 mm space free on each side of the machine and behind for
right ventilation. ▲
Check mains and frequency: they must correspond to values shown on
instrument identification label.
IEC CL10 230 V ±10% 50/60 Hz (1 phase + ground)
IEC CL10 120 V ±10% 60 Hz (1 phase + ground)
IEC CL10 100 V ±10% 50/60 Hz (1 phase + ground)
Check that mains wiring are effectively grounded. ▲
Manufacturer declines all responsibility for any damages due to non-
grounding of the machine.

2-2 IEC CL10 Thermo Electron Corporation
Chapter 2
Installation
• Indoor use.
• Temperature: 5°C to + 40°C.
• Maximum relative humidity of 80% for temperatures up to 31°C
decreasing linearly to 50% relative humidity at 40°C.
• Installation category: II
• Pollution degree: 2
1. Switch the centrifuge on trough the main on the right side of the
unit.
2. When the centrifuge is switched on but stationary: LID led on at
the opening.
3. The mechanic unlocks and consequent opening of the lid is
obtained by pulling the lever on the right side of the machine
towards the centrifuge front.
Warning Before proceeding to installation operations, it is advisable to
disconnect the centrifuge from the mains, by moving the POWER
switch on “O” position. ▲
4. After opening the lid, remove any possible packaging around the
driving spindle. Unscrew the equipment-blocking nut from the
engine shaft.
5. Carefully clean the inside of the centrifugation chamber removing
any packing residues. Due to the air - whirling, solid particles
accidentally left in the centrifugation chamber would create an
excessive wear of the chamber itself and of the outer Equipment
rotating bottoms.
In the event of mains non-availability or power failure, the lid can be
opened by using the special tool supplied with the centrifuge.
This operation should always be effected with the centrifuge mains
plug disconnected.
1. Unscrew the nylon screw in the hole next to opening lever, and
here below identified by the corresponding label.
2. Insert horizontally the unlocking tool supplied with the centrifuge
in the hole (fig. a).
Environmental
conditions
Lid Unlock
Emergency lid unlock

3. Lift the unlocking tool upwards and at the same time pull the lever
(fig. b).
4. At the end screw again the nylon screw.
Warning Should the emergency occur due to electricity failure, during
centrifugation (E4 message will be shown when the power is on again),
wait at least 5 minutes for the end of the Rotating Equipment rotation.
Upon opening, lift the lid by hand and observe the rotor (be careful in
this operation): if it is still rotating, close the lid and wait. ▲
Before any centrifugation run, the rotating equipment should be
thoroughly inspected for corrosion and cleanliness (see 6.3). The clip in
the center of the rotor and the drive spindle should also be clean and
undamaged.
Any failure to follow the above advice may have serious consequences
for the safety of the appliance.
If visible signals of corrosion or anomalies on the rotor or buckets are
present, contact the authorized personnel. Also the centrifuge should
be observed: screw, lid hings, latch and corrosion. In case of problems
contact the technicians. ▲
Figure 1.
Figure 2.
Preliminary
inspection
IEC CL10 2-3Thermo Electron Corporation
Chapter 2
Installation

The rotor’s drive is made by means of one pin inserted in the rotor’s
shaft, which must coincide with the milling of the rotor.
For a right mounting, follow instructions hereafter indicated:
• ensure that both the spindle of the motor and the hole of the head
are clean;
• introduce the head in the centrifuge protective bowl and slip it into
the spindle with the milling of the collar face up;
• align the milling in the collar with the pin on the spindle;
• screw and strongly tighten (with the supplied key) the hexagonal
nut on the threaded spindle of the motor unit (if previous
operations have not performed correctly, this phase will be
impossible as scheduled from the requirements of Safety Standard
EN 61010).
Warning Be sure the rotating head has been correctly locked and the
rotating equipment assembled, before starting the centrifuge. ▲
For rotating equipment with accessible centers, unscrew with the
supplied wrench and lift with the fingers while pulling with the other
hand supporting the rotor.
Assembly of Rotating
Equipment
Rotating head
removing
2-4 IEC CL10 Thermo Electron Corporation
Chapter 2
Installation

IEC CL10 3-1Thermo Electron Corporation
Chapter 3 Characteristics
Height x Width x Depth:................................28 x 37 x 46 cm
Net Weight: ....................................................28 Kg
Max. allowable capacity:..................................with Swing-out rotor
(4 x 135 ml) 540 x 10-6m3
........................................................................with Fixed-angle rotor
(6 x 50 ml) 300 x 10-6m3
Max. allowable density: ..................................1200 Kg/m3
Max. allowable weight:....................................0.648 Kg
Max. speed: ....................................................6500 rpm
Max. RCF at Tip:............................................3684 xg
Work temperature:..........................................Ta+ ∆T
Max. noise:......................................................57 dBA
Nominal operating voltage: ..........230V ± 10% 50/60 Hz
120V ± 10% 60 Hz
100V ± 10% 50/60 Hz
Max peak current: ........................1,5 A (for 230V)
2,5 A (for 120V)
3,8 A (for 100V)
Max power (steady state):..............150 W
• Microprocessor controlled.
• Set/reading speed 300 – 6500 rpm (by step of 10 rpm) for each
pressing of the key; from 300 rpm step of 100 rpm if you press the
key continually more than 2 sec.); accuracy: ± 20 rpm.
• High or low acceleration.
• High or low deceleration.
• Set timer 1 min. up to 99 min. + continuous function.
• Electronic-accelerometric imbalance encoder.
• Direct driving system.
• Brushless induction drive motor.
Dimensions and
Weight
Centrifugation
characteristics
Electrical
characteristics
Specifications