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Leica CM3600 XP User manual

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Leica CM3600 XP
Cryomacrotome
Instructions for Use
English
Order No.: 14 0417 80101 – Revision D
Always keep this manual near the instrument.
Read carefully before working with the instrument.
Version 6.7, Revision D – 12.2018
3
Leica CM3600 XP
The information, numerical data, notes and value judgments contained in this Instructions for Use
represent the current state of scientific knowledge and state-of-the-art technology as we understand
it following thorough investigation in this field.
We are under no obligation to update the present Instructions for Use periodically and on an ongoing
basis according to the latest technical developments, nor to provide our customers with additional
copies, updates etc. of this Instructions for Use.
To the extent permitted in accordance with the national legal system as applicable in each individual
case, we shall not be held liable for erroneous statements, drawings, technical illustrations etc.
contained in this Instructions for Use. In particular, no liability whatsoever is accepted for any financial
loss or consequential damage caused by or related to compliance with statements or other information
in this Instructions for Use.
Statements, drawings, illustrations and other information regarding the contents or technical details of
the present Instructions for Use are not to be considered warranted characteristics of our products.
These are determined only by the contract provisions agreed between ourselves and our customers.
Leica reserves the right to change technical specifications as well as manufacturing processes without
prior notice. Only in this way is it possible to continuously improve the technology and manufacturing
techniques used in our products.
This document is protected under copyright laws. All copyrights to this documentation are held by
Leica Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or of any parts thereof) by means of print, photocopy,
microfiche, web cam or other methods – including any electronic systems and media – requires express
prior permission in writing by Leica Biosystems Nussloch GmbH.
For the instrument serial number and year of manufacture, please refer to the nameplate on the back of
the instrument.
Note
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19
69226 Nussloch
Germany
Tel.: +49 - (0) 6224 - 143 0
Fax: +49 - (0) 6224 - 143 268
Web: www.LeicaBiosystems.com
4Version 6.7, Revision D
Table of contents
1. Important Information ................................................................................................................................ 7
1.1 Symbols and their meaning............................................................................................................................ 7
1.2 Instrument type................................................................................................................................................ 9
1.3 User group ........................................................................................................................................................ 9
1.4 Intended use................................................................................................................................................... 10
2. Characteristics of the Leica CM3600 XP .............................................................................................. 11
2.1 Layout of the Leica CM3600 XP................................................................................................................... 11
2.2 Technical data................................................................................................................................................ 12
2.3 Instrument parts............................................................................................................................................. 14
2.4 Function........................................................................................................................................................... 15
3. Safety .......................................................................................................................................................... 16
3.1 Safety notes.................................................................................................................................................... 16
3.2 Warnings......................................................................................................................................................... 16
3.3 Safety features............................................................................................................................................... 18
3.3.1 Emergency stop switch ................................................................................................................................ 18
3.3.2 Knee lever....................................................................................................................................................... 19
3.3.3 Window ........................................................................................................................................................... 19
3.3.4 Cabinet............................................................................................................................................................. 19
4. Site requirements ..................................................................................................................................... 20
4.1 Site requirements at place of installation.................................................................................................. 20
4.2 Electrical connections.................................................................................................................................. 21
4.3 Other connections......................................................................................................................................... 21
5. Installation ................................................................................................................................................. 22
5.1 Unpacking and installation........................................................................................................................... 22
5.2 Standard delivery........................................................................................................................................... 22
5.3 Port and switch panel................................................................................................................................... 23
5.3.1 Port and switch functions ............................................................................................................................ 24
5.3.2 Fuses................................................................................................................................................................ 25
5.4 PC ..................................................................................................................................................................... 26
6. Software ..................................................................................................................................................... 27
6.1 Start and log-in procedure........................................................................................................................... 27
6.2 Description of the window elements.......................................................................................................... 28
6.3 Initialization..................................................................................................................................................... 30
6.4 Main window.................................................................................................................................................. 31
6.4.1 Chamber temperature................................................................................................................................... 31
6.4.2 Time.................................................................................................................................................................. 32
6.4.3 Automatic defrost.......................................................................................................................................... 32
6.4.4 Automatic dehydration ................................................................................................................................. 32
6.4.5 Knife movement ............................................................................................................................................. 32
6.4.6 Extraction system .......................................................................................................................................... 34
6.4.7 Section program ............................................................................................................................................ 34
6.4.8 Sledge speed.................................................................................................................................................. 35
6.4.9 Operation mode.............................................................................................................................................. 35
6.4.10 Cutting window .............................................................................................................................................. 36
5
Leica CM3600 XP
Table of contents
6.5 Parameter settings........................................................................................................................................ 37
6.5.1 Parameter settings........................................................................................................................................ 38
6.5.2 Password management................................................................................................................................ 41
6.5.3 Configuration.................................................................................................................................................. 43
6.5.4 Reference voltages ....................................................................................................................................... 47
6.5.5 Language selection....................................................................................................................................... 47
6.5.6 Initialization..................................................................................................................................................... 48
6.6 Temperature curves...................................................................................................................................... 48
6.6.1 Work space..................................................................................................................................................... 49
6.6.2 Diagram........................................................................................................................................................... 50
6.7 Chronological event list................................................................................................................................ 51
6.7.1 Work space..................................................................................................................................................... 51
6.7.2 Event list.......................................................................................................................................................... 53
6.8 Alarm list ......................................................................................................................................................... 54
6.8.1 Work space..................................................................................................................................................... 54
6.8.2 Alarm list ......................................................................................................................................................... 55
6.8.3 Error messages: Meaning and troubleshooting....................................................................................... 56
6.9 Chamber illumination .................................................................................................................................... 56
6.10 Section documentation................................................................................................................................. 56
6.10.1 Tree diagram .................................................................................................................................................. 57
6.10.2 Studies protocol............................................................................................................................................. 58
6.11 Photo mode..................................................................................................................................................... 60
6.12 Screen printout .............................................................................................................................................. 61
6.13 Help.................................................................................................................................................................. 61
6.14 Save ................................................................................................................................................................. 61
6.15 Printing labels................................................................................................................................................. 61
6.16 Foot switch...................................................................................................................................................... 61
6.17 User log-in/log-out......................................................................................................................................... 62
6.18 Quitting the application................................................................................................................................. 62
7. Operating the Leica CM3600 XP............................................................................................................. 63
7.1 Check list......................................................................................................................................................... 63
7.2 Switching the instrument on........................................................................................................................ 63
7.3 Starting the software .................................................................................................................................... 63
7.4 Setting the chamber temperature............................................................................................................... 64
7.5 Specimen stages ........................................................................................................................................... 64
7.5.1 Inserting the specimen stage, orientable, with ball-joint ....................................................................... 65
7.5.2 Inserting the specimen stage, non-orientable.......................................................................................... 66
7.6 Knife................................................................................................................................................................. 67
7.6.1 Inserting standard knives............................................................................................................................. 68
7.6.2 Inserting the disposable blade holder with disposable blade ............................................................... 69
7.6.3 Adjusting the knife angle.............................................................................................................................. 70
7.7 Setting the trimming parameters ................................................................................................................ 71
7.8 Setting the sectioning parameters ............................................................................................................. 71
7.8.1 Sectioning the specimen.............................................................................................................................. 71
7.8.2 Collecting the sections ................................................................................................................................. 72
7.9 Dehydrating the sections ............................................................................................................................. 73
6Version 6.7, Revision D
Table of contents
7.10 Manually shifting the knife holder for high specimens........................................................................... 73
7.10.1 User interface................................................................................................................................................. 73
7.10.2 Knife holder description ............................................................................................................................... 74
7.10.3 Knife holder height adjustment ................................................................................................................... 75
7.10.4 Uninstalling the knife holder........................................................................................................................ 75
7.10.5 Sectioning thick blocks................................................................................................................................. 76
8. Cleaning and Maintenance..................................................................................................................... 77
8.1 Cleaning........................................................................................................................................................... 77
8.1.1 Removing the microtome.............................................................................................................................. 77
8.2 Maintenance .................................................................................................................................................. 79
8.2.1 Oiling................................................................................................................................................................ 79
8.2.2 Defrosting........................................................................................................................................................ 80
9. Optional Accessories............................................................................................................................... 81
9.1 Ordering information..................................................................................................................................... 81
10. Warranty and Service .............................................................................................................................. 83
11. Decommissioning and Disposal ............................................................................................................ 84
12. Decontamination Confirmation.............................................................................................................. 85
7
Leica CM3600 XP
Important Information 1
1. Important Information
1.1 Symbols and their meaning
Symbol: Title of the symbol: Warning
Description: Warnings appear in a white box, orange header and
are marked by a warning triangle.
Symbol: Title of the symbol: Note
Description: Notes, i. e. important user information, appear in
a white box, blue header and are marked by an
information symbol.
Symbol: Title of the symbol: Item number
→"Fig. 7-1"Description: Item numbers for numbering illustrations. Numbers in
red refer to item numbers in illustrations.
Symbol: Title of the symbol: Function key
Save Description: Software symbols that have to be pressed on the
input screen are displayed as bold, gray text.
Symbol: Title of the symbol: Keys and switches on the instrument
Start Description: Keys and switches on the instrument that are
supposed to be pressed by the user in various
situations are displayed as bold, gray and underlined
text.
Symbol: Title of the symbol: China ROHS
Description: Environmental protection symbol of the China RoHS
directive. The number in the symbol indicates the
"Environment-friendly Use Period" of the product.
The symbol is used if a substance restricted in China
is used in excess of the maximum permitted limit.
Symbol: Title of the symbol: WEEE Symbol
Description: Symbol for labeling electrical and electronic
equipment in accordance with Section 7 of the
German Electrical and Electronic Equipment Act
(ElektroG). ElektroG is the law regarding the sale,
return and environmentally sound disposal of
electrical and electronic equipment.
Symbol: Title of the symbol: CE Compliance
Description: CE labeling shows that the product corresponds to
one or more applicable European directives.
Symbol: Title of the symbol: Manufacturer
Description: Indicates the manufacturer of the medical product.
8Version 6.7, Revision D
Important Information
1
Symbol: Title of the symbol: Manufacturing date
Description: Indicates the date when the medical device was
manufactured.
Symbol: Title of the symbol: Article number
Description: Indicates the manufacturer's catalog number so that
the medical device can be identified.
Symbol: Title of the symbol: Serial number
Description: Indicates the manufacturer's serial number so that
a specific medical device can be identified.
Symbol: Title of the symbol: Consult Instructions for Use
Description: Indicates the need for the user to consult the
Instructions for Use.
Symbol: Title of the symbol: Fragile, handle with care
Description: The package contents are fragile and must be
handled with care.
Symbol: Title of the symbol: This way up
Description: Indicates correct upright position of the transport
package.
Symbol: Title of the symbol: Store dry
Description: The package must be kept in a dry environment.
Symbol: Title of the symbol: IPPC symbol
Description: The IPPC symbol includes
• IPPC symbol
• Country code in accordance with ISO 3166,
e. g. DE for Germany
• Regional identifier, e. g. HE for Hesse
• Registration number, unique number beginning
with 49.
• Treatment code, e. g. HT (heat treatment), MB
(methyl bromide), and possibly DB (debarked)
Example of labeling
in accordance with
IPPC
9
Leica CM3600 XP
Important Information 1
Appearance: Indication: Tilt indicator
Description: Tip-n-Tell indicator to monitor whether the shipment
has been transported and stored in upright position
according to your requirements. With a tilt of 60°
or more, the blue quartz sand flows into the arrow-
shaped display field and remains there permanently.
Improper handling of the shipment is immediately
detectable and can be proven definitively.
Note
When delivering the instrument, the recipient must check that the tilt indicator is intact. The
responsible Leica representative must be notified in the case that one or all indicators have been
triggered.
1.2 Instrument type
All information provided in these Instructions for Use applies only to the instrument type indicated on
the title page. An nameplate with the serial number is attached on the center on the rear side of the
cabinet.
Note
The exact data of the different instrument versions are specified in Chapter (→p. 12 – 2.2
Technical data).
1.3 User group
Only trained laboratory personnel may operate the Leica CM3600 XP.
Prior to operating the instrument, the operator must carefully read and understand these Instructions
for Use and must familiarize him/herself with all technical details of the instrument.
10 Version 6.7, Revision D
Important Information
1
1.4 Intended use
The Leica CM3600 XP is a PLC-controlled and motorized precision cryomacrotome for largesurface
sections, equipped with a convection-cooled chamber for deep-freezing, sectioning and freeze-drying
large biological and industrial specimens.
The instrument is designed for the aforementioned applications only and must be operated according to
the instructions in these Instructions for Use.
Warning
Any use of the instrument not described in these Instructions for Use is considered improper.
If you wish to use the instrument for special applications or modes of operation not listed in this manual,
it is absolutely necessary that you contact your local Leica representation prior to doing so.
Otherwise, you will loose the Leica warranty on your instrument.
Only trained personnel, skilled in the use of the above-mentioned applications, may operate the
Leica CM3600 XP.
Prior to working with the instrument, each operator must thoroughly read and understand these
Instructions for Use.