Leica CM3600 XP User manual

Leica CM3600 XP
Cryomacrotome
Instructions for Use
English
Order No.: 14 0417 80101 – Revision D
Always keep this manual near the instrument.
Read carefully before working with the instrument.
Version 6.7, Revision D – 12.2018

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Leica CM3600 XP
The information, numerical data, notes and value judgments contained in this Instructions for Use
represent the current state of scientific knowledge and state-of-the-art technology as we understand
it following thorough investigation in this field.
We are under no obligation to update the present Instructions for Use periodically and on an ongoing
basis according to the latest technical developments, nor to provide our customers with additional
copies, updates etc. of this Instructions for Use.
To the extent permitted in accordance with the national legal system as applicable in each individual
case, we shall not be held liable for erroneous statements, drawings, technical illustrations etc.
contained in this Instructions for Use. In particular, no liability whatsoever is accepted for any financial
loss or consequential damage caused by or related to compliance with statements or other information
in this Instructions for Use.
Statements, drawings, illustrations and other information regarding the contents or technical details of
the present Instructions for Use are not to be considered warranted characteristics of our products.
These are determined only by the contract provisions agreed between ourselves and our customers.
Leica reserves the right to change technical specifications as well as manufacturing processes without
prior notice. Only in this way is it possible to continuously improve the technology and manufacturing
techniques used in our products.
This document is protected under copyright laws. All copyrights to this documentation are held by
Leica Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or of any parts thereof) by means of print, photocopy,
microfiche, web cam or other methods – including any electronic systems and media – requires express
prior permission in writing by Leica Biosystems Nussloch GmbH.
For the instrument serial number and year of manufacture, please refer to the nameplate on the back of
the instrument.
Note
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19
69226 Nussloch
Germany
Tel.: +49 - (0) 6224 - 143 0
Fax: +49 - (0) 6224 - 143 268
Web: www.LeicaBiosystems.com

4Version 6.7, Revision D
Table of contents
1. Important Information ................................................................................................................................ 7
1.1 Symbols and their meaning............................................................................................................................ 7
1.2 Instrument type................................................................................................................................................ 9
1.3 User group ........................................................................................................................................................ 9
1.4 Intended use................................................................................................................................................... 10
2. Characteristics of the Leica CM3600 XP .............................................................................................. 11
2.1 Layout of the Leica CM3600 XP................................................................................................................... 11
2.2 Technical data................................................................................................................................................ 12
2.3 Instrument parts............................................................................................................................................. 14
2.4 Function........................................................................................................................................................... 15
3. Safety .......................................................................................................................................................... 16
3.1 Safety notes.................................................................................................................................................... 16
3.2 Warnings......................................................................................................................................................... 16
3.3 Safety features............................................................................................................................................... 18
3.3.1 Emergency stop switch ................................................................................................................................ 18
3.3.2 Knee lever....................................................................................................................................................... 19
3.3.3 Window ........................................................................................................................................................... 19
3.3.4 Cabinet............................................................................................................................................................. 19
4. Site requirements ..................................................................................................................................... 20
4.1 Site requirements at place of installation.................................................................................................. 20
4.2 Electrical connections.................................................................................................................................. 21
4.3 Other connections......................................................................................................................................... 21
5. Installation ................................................................................................................................................. 22
5.1 Unpacking and installation........................................................................................................................... 22
5.2 Standard delivery........................................................................................................................................... 22
5.3 Port and switch panel................................................................................................................................... 23
5.3.1 Port and switch functions ............................................................................................................................ 24
5.3.2 Fuses................................................................................................................................................................ 25
5.4 PC ..................................................................................................................................................................... 26
6. Software ..................................................................................................................................................... 27
6.1 Start and log-in procedure........................................................................................................................... 27
6.2 Description of the window elements.......................................................................................................... 28
6.3 Initialization..................................................................................................................................................... 30
6.4 Main window.................................................................................................................................................. 31
6.4.1 Chamber temperature................................................................................................................................... 31
6.4.2 Time.................................................................................................................................................................. 32
6.4.3 Automatic defrost.......................................................................................................................................... 32
6.4.4 Automatic dehydration ................................................................................................................................. 32
6.4.5 Knife movement ............................................................................................................................................. 32
6.4.6 Extraction system .......................................................................................................................................... 34
6.4.7 Section program ............................................................................................................................................ 34
6.4.8 Sledge speed.................................................................................................................................................. 35
6.4.9 Operation mode.............................................................................................................................................. 35
6.4.10 Cutting window .............................................................................................................................................. 36

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Leica CM3600 XP
Table of contents
6.5 Parameter settings........................................................................................................................................ 37
6.5.1 Parameter settings........................................................................................................................................ 38
6.5.2 Password management................................................................................................................................ 41
6.5.3 Configuration.................................................................................................................................................. 43
6.5.4 Reference voltages ....................................................................................................................................... 47
6.5.5 Language selection....................................................................................................................................... 47
6.5.6 Initialization..................................................................................................................................................... 48
6.6 Temperature curves...................................................................................................................................... 48
6.6.1 Work space..................................................................................................................................................... 49
6.6.2 Diagram........................................................................................................................................................... 50
6.7 Chronological event list................................................................................................................................ 51
6.7.1 Work space..................................................................................................................................................... 51
6.7.2 Event list.......................................................................................................................................................... 53
6.8 Alarm list ......................................................................................................................................................... 54
6.8.1 Work space..................................................................................................................................................... 54
6.8.2 Alarm list ......................................................................................................................................................... 55
6.8.3 Error messages: Meaning and troubleshooting....................................................................................... 56
6.9 Chamber illumination .................................................................................................................................... 56
6.10 Section documentation................................................................................................................................. 56
6.10.1 Tree diagram .................................................................................................................................................. 57
6.10.2 Studies protocol............................................................................................................................................. 58
6.11 Photo mode..................................................................................................................................................... 60
6.12 Screen printout .............................................................................................................................................. 61
6.13 Help.................................................................................................................................................................. 61
6.14 Save ................................................................................................................................................................. 61
6.15 Printing labels................................................................................................................................................. 61
6.16 Foot switch...................................................................................................................................................... 61
6.17 User log-in/log-out......................................................................................................................................... 62
6.18 Quitting the application................................................................................................................................. 62
7. Operating the Leica CM3600 XP............................................................................................................. 63
7.1 Check list......................................................................................................................................................... 63
7.2 Switching the instrument on........................................................................................................................ 63
7.3 Starting the software .................................................................................................................................... 63
7.4 Setting the chamber temperature............................................................................................................... 64
7.5 Specimen stages ........................................................................................................................................... 64
7.5.1 Inserting the specimen stage, orientable, with ball-joint ....................................................................... 65
7.5.2 Inserting the specimen stage, non-orientable.......................................................................................... 66
7.6 Knife................................................................................................................................................................. 67
7.6.1 Inserting standard knives............................................................................................................................. 68
7.6.2 Inserting the disposable blade holder with disposable blade ............................................................... 69
7.6.3 Adjusting the knife angle.............................................................................................................................. 70
7.7 Setting the trimming parameters ................................................................................................................ 71
7.8 Setting the sectioning parameters ............................................................................................................. 71
7.8.1 Sectioning the specimen.............................................................................................................................. 71
7.8.2 Collecting the sections ................................................................................................................................. 72
7.9 Dehydrating the sections ............................................................................................................................. 73