Dynamica VELOCITY 14R Pro User manual

VELOCITY 14R
Pro
Benchtop Refrigerated Centrifuge
Instruction manual
Model VELOCITY 14R Pro Benchtop Refrigerated Centrifuge
V2009

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MODEL VELOCITY 14R Pro
BENCHTOP REFRIGERATED CENTRIFUGE
Thank you very much for choosing Dynamica Velocity 14R Pro centrifuge. Please read
this instruction manual carefully before using the centrifuge.
Appearance or specification is subject to change without notice.
Dynamica

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iii
Safety Reminder
Symbol is the general internationally safety sign, please read carefully and fully
understand the following safety precautions.
⚫Read all safety Warnings and Cautions in this manual carefully.
⚫Safety messages are labeled as followings. The safety symbol is in combination
with words of “WARNING” and “CAUTION” to notify users the potential danger; The
definitions of this two combination are as follows:
WARNING: Personal dangerousness
Warning notes indicate any condition or practice, which if not strictly observed, could
result in personal injury or possible death.
CAUTION: Instrument damage
Caution notes indicate any condition or practice, which if not strictly observed or
remedied, could result in damage or destruction of the equipment.
NOTE: Notes indicate an area or subject of special merit, emphasizing either the
product’s capability or common errors in operation or maintenance.
⚫Do not use the centrifuge in the way which does not mention on this manual, Please
contact Dynamica technician if you have any question.
WARNING:
⚫This centrifuge is not explosion-proof. Never use explosive or flammable samples.
⚫Do not install the centrifuge in or near places where inflammable gases are
generated or chemicals are stored.
⚫Make sure to prepare necessary safely measures before using samples that are
toxic, radioactive or contaminated with pathogenic micro-organisms.
⚫If the instrument, the rotor and or accessories that has been contaminated by
solutions with toxic, radioactive or pathogenic materials, clean it according to the
decontamination procedure that you specified.
⚫If the contaminated equipment requires service of Dynamica or authorized agency
of Dynamica, either at the customer’s site, Dynamica or at the agent facilities, sterilize
and decontaminate it in advance. Make sure to notify the service representatives of the
use of such materials.
⚫Do not touch the power cord or switch with wet hands to avoid electrical shocks.
⚫Users or any hazardous materials are recommended to keep 30cm away from the

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centrifuge when it is operating.
⚫Never forcedly release the door lock while the rotor is rotating.
⚫Unauthorized repairs, disassembly, and other services applied to the centrifuge are
strictly prohibited.
CAUTION:
⚫The centrifuge must be located on the firm and level table.
⚫Be careful not to get your fingers or hands caught between the door hook and the
table when closing the door.
⚫When opening the door, make sure the angle between door and the shell is more
than 70 degree.
⚫Do not move or relocate this centrifuge while the rotor is rotating.
⚫If there is liquid in the chamber, towel off it immediately to avoid contaminating the
sample.
⚫Keep the chamber clean and remove any objects before running the instrument.
⚫Cautions on rotors:
1）Always check for corrosion and damages on the rotor surface before using it. Do
not use the rotor or bucket if such abnormality is found.
2）Do not run this centrifuge over the allowable maximum speed of the rotor, buckets,
and adapters. If their maximum speeds vary, run it at the lowest maximum speed
among them.
3）Do not exceed the allowable imbalance.
4）Make sure the tubes and bottles within their actual capacities.
5）Make sure all the buckets are the same type at all times.
6）If the rotor is provided with a cover, make sure it is tightly rotated on the rotor
before the operation.
7）Use recommended rotors only.
⚫If any abnormal condition occurs during operation, stop it immediately and contact
our service representative. Notify the service representative the error code.
⚫Earthquakes may cause damage to the centrifuge. Contact our service
representative if abnormality observed.

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Contents
Safety Reminder..............................................................................................iii
1 Specification ...........................................................................................................1
2 Operational Condition..........................................................................................2
2.1 Basic operational conditions........................................................................2
2.2 Transport and storage condition..................................................................2
3 Installation...............................................................................................................3
3.1 Location...........................................................................................................3
3.2 Connection of the power cord and grounding...........................................3
4 Structure ..................................................................................................................4
5 Operation panel......................................................................................................5
6 Preparation (Rotor)................................................................................................7
7 Operation .................................................................................................................9
7.1 Normal Operation..........................................................................................9
7.2 RCF Operation.............................................................................................14
7.3 Programmed Operation..............................................................................16
7.4 Pulse Operation...........................................................................................17
7.5 Pre-cooling operation..................................................................................17
7.6 Browse the rotor information......................................................................18
8 Acceleration and Deceleration Rates.............................................................20
9 Temperature Control...........................................................................................21
9.1 Sample temperature during the run..........................................................21
9.2 High-temperature operation.......................................................................21
10 Maintenance........................................................................................................22
10.1 The daily maintenance .............................................................................22
10.2 Periodic inspect and replace consumable parts...................................23
11 Troubleshooting.................................................................................................24
11.1 Common malfunction list..........................................................................24
11.2 Identify the malfunction.............................................................................25
12 Frequent problems and solutions.................................................................26
12.1 How to open the door ...............................................................................26
12.2 How to remove the rotor stuck on the shaft..........................................26
13 Applicable rotors and tubes ...........................................................................28
13.1 Table of applicable rotors.........................................................................28
13.2 Cleaning and sterilizing tubes and bottles.............................................29
14 Rotating radii of applicable rotors................................................................31
15 Calculating relative centrifuge force (RCF)................................................31
16 Circuit connecting graph.................................................................................32
17 Guarantee............................................................................................................33
After-sales Service..................................................................................................33

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1
Maximum speed
14,000rpm
Maximum RCF
21,780×g
Maximum capacity
6×50ml
Timer
1Minute~99hours59Minutes~HOLD (continuous
running)
Settable temperature
range
-20℃~40℃
Acceleration/deceleration
profiles
（1~9）/（1~9）stages (9 is the fastest curve)
Driving system
Brushless DC Motor
Refrigerant
Environment protection refrigerant 134a
Program memory
9
Safety features
Cover door with dual-locks, over-speed protection,
overheat protection and imbalance protection, situation
diagnosis system
Power requirements
220V: Single phase, ~220-240V±10%, 50/60Hz±1Hz,
1500VA
110V: Single phase, ~110V±10%, 60Hz±1Hz, 1500VA
Dimensions（mm）
560mm×500mm×380 mm(L*W*H)
Weight
About 60kg
Additional features
Rotor auto identification, Speed/Acceleration switch
function, Pre-cooling function, Short time running
function, Processing display
1 Specification

2
2 Operational Condition
2.1Basic operational conditions
1) Power: (220V) single phase, ~220-240V±10%, 50/60Hz±1Hz, 10A, standard sine
wave;
(110V) single phase, ~110V±10%, 60Hz±1Hz, 25A, standard sine wave;
Install an emergency switch that turns off the main power supply in the event of
malfunction. It is ideal to install the emergency switch outside of the room or near the exit;
2) Environment temperature: 2℃～40℃;
3) Relative humidity: ≤80%;
4) No vigorous vibration and airflow around;
5) No electric dust, explosive and corrosive gases around;
2.2 Transport and storage condition
1) Storage temperature: -40℃～+55℃;
2) Relative humidity: ≤93%.

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3 Installation
This section describes the instructions that you should abide by when installing the
centrifuge to ensure your safety and the optimum performance. Before moving the
centrifuge, the rotor must be removed.
WARNING:
•This centrifuge may be damaged if it is connected to an improper power source.
•Check if the power source meets the requirements.
3.1 Location
1）Locate the centrifuge on a firm and level table, ensure the feet of the centrifuge stand
on the table firmly. Avoid installing on the slippery table-board that conveys vibration.
2）Ideal environment temperature is 20℃±5℃. Temperature should not be over 30℃.
Avoid direct sunlight to the instrument.
3）Keep clearances of 10 cm on both sides and 30cm behind it to ensure its cooling
efficiency. After installing, this distance should be marked around the centrifuge to ensure
its cooling efficiency.
4）Do not install nearby a heat-generating device or waterworks from where water may
drip or splash. Such location may cause sample temperature fluctuation or malfunction of
the centrifuge.
WARNING: No any hazardous materials are within 30cm around the centrifuge to
avoid leaking due to heavy vibration of the centrifuge.
3.2 Connection of the power cord and grounding
WARNING:
•Do not touch the power cord with wet hands to avoid electrical shocks.
•This centrifuge must be properly grounded.
1) This centrifuge is equipped with a 3P flat plug. Grounding can be done by plugging the
3P plug into the outlet.
2) The outlet must have bigger current capacity than 10Aand its earth terminal is installed
properly.

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4 Structure
Front Rear
Inner Chamber
Door
Front board
Link
Power wire
Fan cover
Lock hook
Door seal
Operation panel
Centrifugal chamber
Knighthead
Drive shaft
Lock hole rubber
Observation window

5
5 Operation panel
Figure 5-1 Operation Panel
NO.
Symbol
Name
Function
⑴
LCD Screen
Displays running parameters and state(Figure 5-2)
⑵
Time button
Set a running time（1min~99hours59min~HOLD）
⑶
Temperature
button
Set a control temperature of the sample
⑷
Speed/RCF
button
Set speed or RCF
⑸
Deceleration
button
Set a deceleration rate. (1-9, 9 is the fastest)
⑹
Acceleration
button
Set an acceleration rate. (1-9, 9 is the fastest)
⑺
Increasing
button
Increase parameter values
⑻
Decreasing
button
Decrease parameter values
⑼
Programmed
button
Store and recall running conditions（0-9groups）
⑽
Stop button
Make the Rotor stop rotating. The red lamp blinks

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while decelerating and quenches when the rotor
stops rotating.
⑾
Start button
Make the rotor start spinning. The green lamp blinks
while accelerating and keeps lighting when the
speed reaches the set value.
⑿
Pulse button
Accelerate the rotor while this button is pressed.
The rotor slow down and stop while this button is
released.
⒀
Pre-cool
button
Press this button and turn into precooling mode.
Press the button for 6 seconds, the error message
list will be displayed.
⒁
Rotor button
Input the rotor number or inquire the rotor
parameters
⒂
Door button
Door lock is released when this button is pressed.
The LCD screen display the main interface as figure 5-2. The left part shows parameters
of the rotary speed, the temperature and the time. The right part shows the operation
status of acceleration, deceleration, rotor ID number and program group etc. Each left
parameter consists of a symbol, a value and a unit, while each of the right status consists
of only a symbol and a value.
The interface displays the operation parameters when the rotor is running, these
parameters can be modified only when the rotor stops or it reaches the setting point.
Please refer Section 7 for detail.
Figure 5-2 The LCD main interface
9
14000 rpm
4℃
2:30 h:m
19
9
7

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6 Preparation (Rotor)
WARNING:
•This centrifuge and the rotor are not explosion-proof. Never use explosive or
flammable samples.
•There are restriction on the usage of biological samples and radioactive
substances that require biological isolation such as pathogens and recombinant DNA
for safety purposes. User must prepare necessary safety measures before treating with
samples containing such substances.
1) Prepare the sample
2) Put the sample into tubes or bottles
CAUTION: Sample may leak from the gap between the bottle and its cap if the
bottle is fully filled up.
◼Do not exceed the capacity specified in the instruction manual.
3) Balance the centrifuge tubes or bottles
CAUTION: There are some cases where samples of different composition due in
different precipitation levels by centrifugation even if they are equal in volume, and such
operation may increase the level of imbalance. Properly arrange the tubes or bottles so
that the same sample is placed symmetrically.
◼See Table 6.1 for allowable imbalance tolerance of each rotor.
◼Although this centrifuge can accept sample balancing by eye, we recommend that the
sample is well-balanced to prolong the life expectancy of the centrifuge.
◼Never intentionally run the centrifuge under unbalanced condition even though the
imbalance tolerance is not exceeded.
4) Inspect the rotor
CAUTION:
⚫If any abnormality such as corrosion or scratches is found, stop using the rotor and
contact our service representative.
⚫Only use the rotors we recommended.
◼Check whether the rotor and the buckets have corrosion or scratch before use.
◼Check whether the swinging bucket rotor swing smoothly by slightly spinning the rotor
manually. Perform periodic maintenance on the rotor.
5) Set balanced tubes or bottles symmetrically on the rotor or rotor frame

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CAUTION: Make sure that the cover is put on the rotor and locked securely.
Otherwise, the rotor or its cover may be dislocated while rotating and damage the
centrifuge or the rotor.
6) Confirm the ID code of the rotor
◼This instrument can identify rotors automatically.
◼Each rotor is assigned with an ID code. After the rotor is identified, the optimum
temperature can be automatically controlled, and the function of over-speed protection
and the speed/RCF display can be realized.
◼The list of available rotor’s ID code, see Table 6.1.
Table 6.1 List of rotors
Rotor type
ID
code
Max.
speed
(rpm)
Max.
RCF
(×g)
Tube/bottle
Imbalance tolerance(*)
Mass
Imbalance
Capacity
Imbalance(**)
FA15A
19
14,000
18,728
1.5/2 ml tube
1.5g/ tube
5mm/ tube
FA12A
03
12,000
14,100
0.2ml microtube
0.2 g/tube
FA15G
09
14,000
21,780
50ml TC tube
3.0 g/ tube
5mm/ tube
FA15B
16
14,000
18,990
50ml tube
3.0g/ tube
5mm/ tube
FA15C
18
14,000
20,290
FA14C
13
14,000
20,920
50ml tube
3.0g/ tube
5mm/ tube
SW4C
05
4,000
1,780
5ml vacutainer for
blood specimen
collection
1.0g/ tube
2mm/ tube
FA18C
01
14,000
17,530
10ml tube
1.5 g/ tube
10mm/ tube
FA15E
08
14,000
19,700
5ml tube with V
bottom
1.0g/tube
2mm/tube
FA15F
07
14,000
19,700
5ml tube with round
bottom
1.0g/tube
2mm/tube
FA12B
02
12,000
13,840
1.5/2 ml tube
1.5g/ tube
5mm/ tube
* : The imbalance tolerance given in the table indicate the mass imbalance or capacity
imbalance when the centrifuge tubes are place symmetrically.
** : Capacity imbalance provide a rough measure of balancing and it is not necessarily to
agree with mass imbalance.

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7 Operation
WARNING:
•Never open the door while the rotor is rotating or touch the rotating rotor.
•For your safety, do not step into the area with 30cm around the centrifuge while it is
running. Users or any hazardous are recommend keeping 30 cm away from the
centrifuge when it is running.
7.1 Normal Operation
1) Turn on the power switch.
◼The LCD screen displays a welcome interface and shows the accumulative total
running time, in the meantime, this centrifuge starts a self-checking process for
preparation. (see Figure 7-1).
Initializing…
V X.XX
Figure 7-1 Initialization interface
◼If the self-checking fails, the screen displays an error code on its right bottom corner.
Please refer the error code in the table 11-1.
NOTE: When the power is turned on, this centrifuge will take 7 seconds to self-check.
During this time, the centrifuge will have no response to the buttons.
◼This centrifuge will move to a preparing interface after self-checking, and display the
running parameters of the last time. For example, the Figure 7-2 shows that the speed
was set to be 14000rpm, the temperature was 4℃, the running time was 2 hours and 30
minutes, the acceleration rate was 9, the deceleration rate was 7, the rotor ID was 19, and
the program group number was 9.
Figure 7-2 is only an example. User’s setting parameters and running parameters may
be different from it.

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Figure 7-2 Preparation mode interface
◼The door is lock automatically.
After self-checking has passed, user can open the door by pressing button. After 6
seconds, door lock will close automatically.
2) Gently lift up the door and set the rotor on the drive shaft.
◼Place the rotor in position until it contacts the drive shaft.
◼You should feel a click when the rotor is properly placed on the drive shaft. If you do
not feel anything, there may be something (e.g. dust) stuck between the rotor and the
drive shaft and the rotor may be tilted. Check and clean the rotor or the shaft if needed.
◼Rotate the rotor slightly with your fingers. If the rotor vibrates obviously, place the
rotor again.
3) Close the door and start running.
CAUTION:
•Make sure that the angle between the door and the table is more than 70 degrees,
otherwise the door may fall down.
•Be carefully not to get your fingers or hands caught between the door hook and
table when closing the door.
4) Set the operating parameter
CAUTION: Some buckets and adapters, and tubes, bottles and microplates that
are sold on the market have lower allowable speeds(allowable RCF) than the rotor. Use
them at the lowest allowable speed or less.
(1) Select a rotor ID.
①Press the button.
◼The rotor symbol blinks, the rotor ID can be selected.
②Press or button to select the rotor ID.
◼The rotor ID code is given in Table 6.1or it can be obtained by rotor inquiring function
14000 rpm
4
2:30 h:m
97
19
9

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(refer to 7.6 section).
NOTE: This step can be omitted because all rotors are automatically recognized.
(2) Set the speed, running time, sample temperature, acceleration and deceleration rate.
①Press button.
◼Speed symbol blinks.
When the speed unit is RCF, it indicates you can input required RCF value. Then, press
the button again, the speed unit becomes rpm, now you can input speed value. In
the same way, you can also change rpm into RCF.
◼If no button is pressed after the symbol has been blinking for 7 seconds, the
symbol will stop blinking, close the inputting mode and return to the preparation screen.
②Press and buttons to adjust the parameter.
◼The minimum speed you can set is 300 rpm with the interval of 100rpm.
◼The minimum RCF you can set is 100×g with the interval of 50×g.
◼When keep pressing and buttons, you can set the values in a fast mode.
◼There is a cycling function on and buttons. Press the button, the
value will go from small value to bigger one, and reach the maximum, then turn to the
minimum, while press button, the sequence is big value, the smaller one, then the
minimum and the maximum.
NOTE: The centrifuge may shake slightly when it is operated under 2,600rpm, this
does not indicate any problem.
③Press the button to set sample temperature.
◼When the temperature symbol blinks, the temperature is ready to be set.
◼Press or button to set the temperature value, the range is from -20℃to
40℃with the interval of 1℃.
④Press button to set running time.
◼While the time symbol blinks, the running time can be set.
◼Press or button to set the running time, the range is from 1minute to 99

12
hours and 59minutes~HOLD with the interval of 1 minute.
⑤Press button to set acceleration rate.
◼While the acceleration symbol blinks, the acceleration rate can be set.
◼Press or button to set the acceleration rate, the range is from 1 to 9 with
the interval of 1. Curve 9 is the highest rate.
⑥Press button to set deceleration rate.
◼While the deceleration symbol blinks, the deceleration rate can be set.
◼Press and buttons to set the deceleration rate, the range is from 1 to 9
with the interval of 1. Curve 9 is the fastest rate.
5) Start running.
(1) Press button, to check the setting values again.
◼Press button for the first time, the screen displays the parameter verification
screen. (see as the following interface)
Figure 7-3 Verification of the operation parameters
◼If something is wrong with the operation parameter, press button, the centrifuge
will return to the preparation mode for correction or modification.
◼If no button is pressed for 7 seconds, the centrifuge will return to the preparation
mode.
(2) Press button again, start running.
◼The door should be locked before the rotor starts rotating.
◼During the acceleration, the green lamp on button keeps blinking. After the
speed reaches set value, the lamp stops blinking and keeps lighting.
14000 rpm
4
02:30 h:m
Continue?
(Y/START, N/STOP)
97

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◼After accelerating for 8 seconds, the interface will display the processing screen, see
the Figure 7-4.
Figure 7-4 Processing Screen
The running status displays the whole process while the centrifuge operates. In this
screen, the left part shows the actual running parameters, the time value is the actual
operation time, not include the acceleration time. The right part is a sign of trapezium area
which is being filled, indicating the whole process of the acceleration stage, the stable
stage, and the deceleration stage. In the mean while, the trapezium also shows the
running percentage. It also shows the running percentage. This screen makes it easier for
the operator to monitor the whole running process.
This running status can not be shown in the pre-cooling mode or the short run mode.
Under the short run mode, the right part of this screen displays the waving bar, and the
HOLD symbol.
(3) Browse and modify the running parameters
◼You can inquire and modify the operation parameters after the centrifuge reaches its
setting speed. Press the corresponding button, the Screen will return to the preparation
screen and displays all setting values, refer to the section 7.1-4 for detail operations of
modifying the parameters. If no button is pressed again, the centrifuge will return to its
normal operation and operate in new parameters 6 seconds later.
◼The rotor ID cannot be modified when the rotor is running. After the time has been
modified, the running time will not be reset to zero whereas it will continue to accumulate,
and the running status screen will adjust accordingly.
(4) Error display
◼If there is anything wrong with the centrifuge, it will brake automatically and display
the error number on the right bottom of the Screen. User can look up the error in the Table
11-1 and make corrective actions accordingly.
WARNING: Do not open the door before the rotor stops.
6) Ending the running
14000 rpm
4
00:30 h:m 20%